MD06G1229700.v1.1
MYB Family

Myb-related protein 308-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001619
Physical Location & Seq
Forward (+)
35927362 .. 35929178
1817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1229700.v1.1.491

Sequence Viewer

Length: 600 bp
ATGAGGAAACCTTGCTGTGAGAAGAGAAAGACGAACAAAGGAGCATGGTCGAAGCAAGAAGATGAGAAGCTCACTCAATATGTTGAAAAAAATGGCGAAGGAAGCTGGCGTTCCCTTCCACTAGCTGCAGGCTTGCTTCGTTGTGGAAAGAGCTGTAGATTAAGATGGGTAAATTATCTAAGGCCAAACGTGAAACGTGGCAACTTTGGTGAAGATGAAGAGGACCTAATCATCAGGCTCCATGCACTTCTTGGAAATAGGTGGTCGTTAATTGCTGGAAGATTGCCGGGACGAACTGACAATGAAGTGAAGAATTACTGGAACACTCATCTAAGGAGAAAACTAATACAAATGGGAGTTGACCCCAATAACCATCGCATAGGACACACACACAATATTGGCCTTACAAAACCATCATTTGGCTCCAGAAAAGTAAATTATCCCTCTAAACCTGTTAATTCCCAAGGGGATAAAGACTCTGATCACATTAAACCATTGTCAGATTCTACCAGTGGCCCGGAAAGCAACACAAGCTGCAGTGGTTTGCCTGACCTCAATCTTGATCTCACGATTGGCCCTCTAAGTTCATTCGGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

200

Amino Acids

22.42

Weight (kDa)

9.6

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 13 - 60 1.3e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 16 - 74 6.2e-11 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 66 - 111 1.9e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 114 3.2e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 419
AfiI CCNNNNNNNGG 1 cut(s) 419
AgsI TTSAA 1 cut(s) 86
AluBI AGCT 5 cut(s) 70, 105, 125, 153, 534
AluI AGCT 5 cut(s) 70, 105, 125, 153, 534
AoxI GGCC 4 cut(s) 182, 400, 514, 574
ApeKI GCWGC 2 cut(s) 125, 534
AspS9I GGNCC 3 cut(s) 223, 515, 575
AsuC2I CCSGG 2 cut(s) 288, 518
AsuHPI GGTGA 1 cut(s) 221
AvaII GGWCC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 112, 521
BccI CCATC 3 cut(s) 159, 381, 421
BciVI GTATCC 1 cut(s) 587
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 2 cut(s) 288, 518
BfaI CTAG 1 cut(s) 122
BfmI CTRYAG 3 cut(s) 126, 154, 535
BfuI GTATCC 1 cut(s) 587
BisI GCNGC 2 cut(s) 126, 535
BlsI GCNGC 2 cut(s) 127, 536
Bme1390I CCNGG 2 cut(s) 288, 518
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 223, 515, 575
BmiI GGNNCC 2 cut(s) 239, 424
BmrFI CCNGG 2 cut(s) 288, 518
BpmI CTGGAG 1 cut(s) 409
BpuMI CCSGG 2 cut(s) 288, 518
BsaJI CCNNGG 1 cut(s) 463
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse1I ACTGG 2 cut(s) 323, 510
BseDI CCNNGG 1 cut(s) 463
BseLI CCNNNNNNNGG 1 cut(s) 419
BseNI ACTGG 2 cut(s) 323, 510
BseXI GCAGC 2 cut(s) 112, 521
BshFI GGCC 4 cut(s) 184, 402, 516, 576
BsiSI CCGG 2 cut(s) 287, 518
BslFI GGGAC 1 cut(s) 303
BslI CCNNNNNNNGG 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 303
BsnI GGCC 4 cut(s) 184, 402, 516, 576
Bsp143I GATC 2 cut(s) 481, 562
BspANI GGCC 4 cut(s) 184, 402, 516, 576
BspLI GGNNCC 2 cut(s) 239, 424
BspMAI CTGCAG 2 cut(s) 130, 539
BsrI ACTGG 2 cut(s) 323, 510
BssECI CCNNGG 1 cut(s) 463
BssMI GATC 2 cut(s) 481, 562
BssT1I CCWWGG 1 cut(s) 463
Bst6I CTCTTC 2 cut(s) 17, 213
BstC8I GCNNGC 3 cut(s) 107, 130, 134
BstDEI CTNAG 3 cut(s) 179, 332, 581
BstKTI GATC 2 cut(s) 484, 565
BstMBI GATC 2 cut(s) 481, 562
BstMWI GCNNNNNNNGC 3 cut(s) 102, 522, 531
BstSCI CCNGG 2 cut(s) 286, 516
BstSFI CTRYAG 3 cut(s) 126, 154, 535
BstV1I GCAGC 2 cut(s) 112, 521
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 4 cut(s) 184, 402, 516, 576
BtgZI GCGATG 1 cut(s) 359
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 2 cut(s) 517, 544
Cac8I GCNNGC 3 cut(s) 107, 130, 134
Cfr13I GGNCC 3 cut(s) 223, 515, 575
CviAII CATG 2 cut(s) 45, 242
DdeI CTNAG 3 cut(s) 179, 332, 581
DpnI GATC 2 cut(s) 483, 564
DpnII GATC 2 cut(s) 481, 562
Eam1104I CTCTTC 2 cut(s) 17, 213
EarI CTCTTC 2 cut(s) 17, 213
Eco130I CCWWGG 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 463
ErhI CCWWGG 1 cut(s) 463
FaeI CATG 2 cut(s) 48, 245
FaiI YATR 4 cut(s) 46, 81, 243, 380
FaqI GGGAC 1 cut(s) 303
FatI CATG 2 cut(s) 44, 241
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 2 cut(s) 126, 535
Fsp4HI GCNGC 2 cut(s) 126, 535
FspBI CTAG 1 cut(s) 122
GluI GCNGC 2 cut(s) 126, 535
GsuI CTGGAG 1 cut(s) 409
HaeIII GGCC 4 cut(s) 184, 402, 516, 576
HapII CCGG 2 cut(s) 287, 518
Hin1II CATG 2 cut(s) 48, 245
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 2 cut(s) 476, 503
HpaII CCGG 2 cut(s) 287, 518
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 3 cut(s) 481, 502, 593
Hpy188III TCNNGA 3 cut(s) 426, 560, 568
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 2 cut(s) 92, 125
HpyCH4IV ACGT 2 cut(s) 189, 196
HpyCH4V TGCA 3 cut(s) 128, 245, 537
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 522, 531
HpyF3I CTNAG 3 cut(s) 179, 332, 581
HpySE526I ACGT 2 cut(s) 189, 196
Hsp92II CATG 2 cut(s) 48, 245
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 2 cut(s) 481, 562
LmnI GCTCC 3 cut(s) 41, 243, 428
Lsp1109I GCAGC 2 cut(s) 112, 521
MaeI CTAG 1 cut(s) 122
MaeII ACGT 2 cut(s) 189, 196
MalI GATC 2 cut(s) 483, 564
MboI GATC 2 cut(s) 481, 562
MboII GAAGA 6 cut(s) 34, 71, 224, 230, 291, 322
MluCI AATT 5 cut(s) 172, 270, 313, 436, 457
MlyI GAGTC 1 cut(s) 470
MnlI CCTC 4 cut(s) 214, 454, 563, 588
MseI TTAA 4 cut(s) 161, 269, 456, 489
MspI CCGG 2 cut(s) 287, 518
MspR9I CCNGG 2 cut(s) 288, 518
MwoI GCNNNNNNNGC 3 cut(s) 102, 522, 531
NciI CCSGG 2 cut(s) 288, 518
NdeII GATC 2 cut(s) 481, 562
NlaIII CATG 2 cut(s) 48, 245
NlaIV GGNNCC 2 cut(s) 239, 424
PfeI GAWTC 1 cut(s) 503
PflMI CCANNNNNTGG 1 cut(s) 419
PkrI GCNGC 2 cut(s) 127, 536
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
PspN4I GGNNCC 2 cut(s) 239, 424
PspPI GGNCC 3 cut(s) 223, 515, 575
PspPPI RGGWCCY 1 cut(s) 223
PstI CTGCAG 2 cut(s) 130, 539
SaqAI TTAA 4 cut(s) 161, 269, 456, 489
SatI GCNGC 2 cut(s) 126, 535
Sau3AI GATC 2 cut(s) 481, 562
Sau96I GGNCC 3 cut(s) 223, 515, 575
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 288, 518
SfcI CTRYAG 3 cut(s) 126, 154, 535
SinI GGWCC 1 cut(s) 223
Sse9I AATT 5 cut(s) 172, 270, 313, 436, 457
SspI AATATT 1 cut(s) 397
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 2 cut(s) 286, 516
StyI CCWWGG 1 cut(s) 463
TaiI ACGT 2 cut(s) 192, 199
TaqI TCGA 1 cut(s) 50
TasI AATT 5 cut(s) 172, 270, 313, 436, 457
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 4 cut(s) 161, 269, 456, 489
Tru9I TTAA 4 cut(s) 161, 269, 456, 489
TscAI CASTG 2 cut(s) 517, 544
TseI GCWGC 2 cut(s) 125, 534
TspDTI ATGAA 3 cut(s) 231, 318, 576
TspRI CASTG 2 cut(s) 517, 544
Van91I CCANNNNNTGG 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 223
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XspI CTAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.