MD14G1234600.v1.1
MYB Family

Myb-related protein 308-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001619
Physical Location & Seq
Forward (+)
31430302 .. 31432320
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1234600.v1.1.491

Sequence Viewer

Length: 591 bp
ATGAGGAAACCTTGCTGTGAGAAGAAAAAGACGAACAAAGGAGCATGGTCGAAGCAAGAAGATGAGAAGCTCACTGAATTTGTTGAAAAAAATGGCGAAGGAAGCTGGCGCTCCCTTCCACTTGCTGCAGGCTTGCTCCGTTGTGGAAAGAGCTGTAGATTAAGATGGGTAAATTATCTAAGGCCAAACTTGAAACGTGGCAACTTTGGTGAAGATGAAGAGGACCTAATCATCAAGCTCCATGCACTTCTTGGAAACAGGTGGTCGCTAATTGCTGGAAGATTGCCGGGACGAACTGACAATGAAGTGAAGAATTACTGGAACACTCATCTAAGGAGAAAACTAATACAAATGGGAGTTGACCCCAATAACCATCGCATAGGACACACACAGAATATTGGCCTTTCAAAATCATCATTTGGCTCCAGAAAAGCAAATCATCCATGCAAAGCCGCGAATTCTCAAGGGGATAACGACTCTGATGATCATCAGAAACCATTTACAGATTCTGCAAGCGGCCCGGAAAGCAACACAAGCTGCAGTGGTTTGCCTGACCTTAATCTTGATCTCACAATAGGCCTTCCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

197

Amino Acids

21.92

Weight (kDa)

9.28

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 13 - 60 3.1e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 16 - 74 5.3e-10 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 66 - 111 1.3e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 114 2e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 455
AciI CCGC 2 cut(s) 453, 516
AcsI RAATTY 2 cut(s) 77, 457
AgsI TTSAA 3 cut(s) 86, 193, 408
AluBI AGCT 5 cut(s) 70, 105, 153, 238, 537
AluI AGCT 5 cut(s) 70, 105, 153, 238, 537
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 4 cut(s) 182, 400, 517, 577
ApeKI GCWGC 2 cut(s) 125, 537
ApoI RAATTY 2 cut(s) 77, 457
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 223, 518
AsuC2I CCSGG 2 cut(s) 288, 521
AsuHPI GGTGA 1 cut(s) 221
AvaII GGWCC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 112, 524
BccI CCATC 2 cut(s) 159, 381
BclI TGATCA 1 cut(s) 484
BcnI CCSGG 2 cut(s) 288, 521
BfmI CTRYAG 3 cut(s) 126, 154, 538
BfoI RGCGCY 1 cut(s) 112
BisI GCNGC 4 cut(s) 126, 453, 517, 538
BlsI GCNGC 4 cut(s) 127, 454, 518, 539
Bme1390I CCNGG 2 cut(s) 288, 521
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 223, 518
BmiI GGNNCC 1 cut(s) 424
BmrFI CCNGG 2 cut(s) 288, 521
BpmI CTGGAG 1 cut(s) 409
BpuEI CTTGAG 1 cut(s) 447
BpuMI CCSGG 2 cut(s) 288, 521
BsaBI GATNNNNATC 1 cut(s) 486
Bse1I ACTGG 1 cut(s) 323
Bse8I GATNNNNATC 1 cut(s) 486
BseGI GGATG 1 cut(s) 439
BseJI GATNNNNATC 1 cut(s) 486
BseNI ACTGG 1 cut(s) 323
BseXI GCAGC 2 cut(s) 112, 524
Bsh1236I CGCG 1 cut(s) 455
BshFI GGCC 4 cut(s) 184, 402, 519, 579
BsiSI CCGG 2 cut(s) 287, 521
BslFI GGGAC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 303
BsnI GGCC 4 cut(s) 184, 402, 519, 579
Bsp143I GATC 2 cut(s) 484, 565
BspACI CCGC 2 cut(s) 453, 516
BspANI GGCC 4 cut(s) 184, 402, 519, 579
BspFNI CGCG 1 cut(s) 455
BspLI GGNNCC 1 cut(s) 424
BspMAI CTGCAG 2 cut(s) 130, 542
BsrI ACTGG 1 cut(s) 323
BssMI GATC 2 cut(s) 484, 565
Bst6I CTCTTC 1 cut(s) 213
BstC8I GCNNGC 4 cut(s) 107, 130, 134, 514
BstDEI CTNAG 2 cut(s) 179, 332
BstF5I GGATG 1 cut(s) 439
BstFNI CGCG 1 cut(s) 455
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 2 cut(s) 487, 568
BstMBI GATC 2 cut(s) 484, 565
BstMWI GCNNNNNNNGC 3 cut(s) 102, 525, 534
BstSCI CCNGG 2 cut(s) 286, 519
BstSFI CTRYAG 3 cut(s) 126, 154, 538
BstUI CGCG 1 cut(s) 455
BstV1I GCAGC 2 cut(s) 112, 524
BsuRI GGCC 4 cut(s) 184, 402, 519, 579
BtgZI GCGATG 1 cut(s) 359
BtsCI GGATG 1 cut(s) 439
BtsI GCAGTG 1 cut(s) 547
BtsIMutI CAGTG 2 cut(s) 72, 547
Cac8I GCNNGC 4 cut(s) 107, 130, 134, 514
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 1 cut(s) 111
Cfr13I GGNCC 2 cut(s) 223, 518
CviAII CATG 3 cut(s) 45, 242, 444
DdeI CTNAG 2 cut(s) 179, 332
DpnI GATC 2 cut(s) 486, 567
DpnII GATC 2 cut(s) 484, 565
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco147I AGGCCT 1 cut(s) 579
Eco47I GGWCC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 223
EcoRI GAATTC 1 cut(s) 457
FaeI CATG 3 cut(s) 48, 245, 447
FaiI YATR 4 cut(s) 46, 243, 380, 445
FaqI GGGAC 1 cut(s) 303
FatI CATG 3 cut(s) 44, 241, 443
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 4 cut(s) 126, 453, 517, 538
FokI GGATG 1 cut(s) 426
Fsp4HI GCNGC 4 cut(s) 126, 453, 517, 538
GlaI GCGC 1 cut(s) 110
GluI GCNGC 4 cut(s) 126, 453, 517, 538
GsuI CTGGAG 1 cut(s) 409
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 4 cut(s) 184, 402, 519, 579
HapII CCGG 2 cut(s) 287, 521
HhaI GCGC 1 cut(s) 111
Hin1II CATG 3 cut(s) 48, 245, 447
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 2 cut(s) 476, 506
HpaII CCGG 2 cut(s) 287, 521
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 2 cut(s) 481, 492
Hpy188III TCNNGA 2 cut(s) 426, 563
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 3 cut(s) 92, 125, 590
HpyCH4IV ACGT 1 cut(s) 196
HpyCH4V TGCA 5 cut(s) 128, 245, 447, 512, 540
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 525, 534
HpyF3I CTNAG 2 cut(s) 179, 332
HpySE526I ACGT 1 cut(s) 196
Hsp92II CATG 3 cut(s) 48, 245, 447
HspAI GCGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 2 cut(s) 484, 565
LmnI GCTCC 5 cut(s) 41, 116, 141, 243, 428
LpnPI CCDG 9 cut(s) 91, 114, 244, 261, 300, 304, 439, 534, 564
Lsp1109I GCAGC 2 cut(s) 112, 524
MaeII ACGT 1 cut(s) 196
MalI GATC 2 cut(s) 486, 567
MboI GATC 2 cut(s) 484, 565
MboII GAAGA 6 cut(s) 34, 71, 224, 230, 291, 322
MluCI AATT 5 cut(s) 77, 172, 270, 313, 457
MlyI GAGTC 1 cut(s) 470
MnlI CCTC 1 cut(s) 214
MseI TTAA 3 cut(s) 161, 558, 589
MspI CCGG 2 cut(s) 287, 521
MspR9I CCNGG 2 cut(s) 288, 521
MvnI CGCG 1 cut(s) 455
MwoI GCNNNNNNNGC 3 cut(s) 102, 525, 534
NciI CCSGG 2 cut(s) 288, 521
NdeII GATC 2 cut(s) 484, 565
NlaIII CATG 3 cut(s) 48, 245, 447
NlaIV GGNNCC 1 cut(s) 424
PceI AGGCCT 1 cut(s) 579
PfeI GAWTC 1 cut(s) 506
PkrI GCNGC 4 cut(s) 127, 454, 518, 539
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 424
PspPI GGNCC 2 cut(s) 223, 518
PspPPI RGGWCCY 1 cut(s) 223
PstI CTGCAG 2 cut(s) 130, 542
PstNI CAGNNNCTG 1 cut(s) 509
SaqAI TTAA 3 cut(s) 161, 558, 589
SatI GCNGC 4 cut(s) 126, 453, 517, 538
Sau3AI GATC 2 cut(s) 484, 565
Sau96I GGNCC 2 cut(s) 223, 518
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 288, 521
SfcI CTRYAG 3 cut(s) 126, 154, 538
SinI GGWCC 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
Sse9I AATT 5 cut(s) 77, 172, 270, 313, 457
SseBI AGGCCT 1 cut(s) 579
SsiI CCGC 2 cut(s) 453, 516
SspI AATATT 1 cut(s) 397
StuI AGGCCT 1 cut(s) 579
StyD4I CCNGG 2 cut(s) 286, 519
TaiI ACGT 1 cut(s) 199
TaqI TCGA 1 cut(s) 50
TasI AATT 5 cut(s) 77, 172, 270, 313, 457
TauI GCSGC 2 cut(s) 455, 519
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 3 cut(s) 161, 558, 589
Tru9I TTAA 3 cut(s) 161, 558, 589
TscAI CASTG 2 cut(s) 79, 547
TseI GCWGC 2 cut(s) 125, 537
TspDTI ATGAA 2 cut(s) 231, 318
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 2 cut(s) 79, 547
VpaK11BI GGWCC 1 cut(s) 223
XapI RAATTY 2 cut(s) 77, 457
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.