MD14G1234500.v1.1
MYB Family

Myb-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001619
Physical Location & Seq
Forward (+)
31422058 .. 31425370
3313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1234500.v1.1.491

Sequence Viewer

Length: 561 bp
ATGAGGAAGCCTGGTTGTGATAAGATAGAGACGAACAAAGGAGCATGGTCTAAGCAAGAAGATCAGAAACTGATTGACTACATCCAGAAACACGGTGAAGGTCGTTGGAATTCTCTTCCTAAGGCTGCAGGGTTGCGTCGGTGTGGCAAAAGTTGTCGACTGAGATGGATAAACTATCTAAGGCCAGATCTTAAACGCGGAAGTATTGGAGAAGATGAAGAGGACCTCATCATCAGGCTTCATGCACTCCTGGGGAATCGGTGGTCACTGATAGCCGGAAGACTGCCGGGAAGGACAGACAATGAGGTGAAGAATTACTGGAACTCTCATATAAGGAAAAAATTATCAAAGATGGGAAGTACTCTTGATCCCAAAAAACCCCATGACCACCATGATCCTCACTTGAGAAGAGGCACTACTGCTGGTGTACCAGTTCTGCAGCCTGATGCTAGAAAACCAATATTATTTGCGTCGTCGTCGTCGGATTCCATGAGTACTGGTGCTAAAATTCACAGCAACGAAAGCGGTTTGCCTGACTTGAATCTCGACCTCTCACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

187

Amino Acids

20.99

Weight (kDa)

9.76

Isoelectric Point (pI)

55.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 13 - 60 7.2e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 16 - 73 1e-11 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 66 - 110 9.4e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 117 1.4e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 157
AccII CGCG 1 cut(s) 198
AciI CCGC 2 cut(s) 198, 525
AclWI GGATC 2 cut(s) 362, 389
AcsI RAATTY 2 cut(s) 109, 507
AfaI GTAC 3 cut(s) 361, 429, 496
AgsI TTSAA 1 cut(s) 541
AjnI CCWGG 2 cut(s) 10, 249
Alw26I GTCTC 1 cut(s) 23
AlwI GGATC 2 cut(s) 362, 389
AlwNI CAGNNNCTG 1 cut(s) 70
AoxI GGCC 1 cut(s) 182
ApeKI GCWGC 2 cut(s) 125, 439
ApoI RAATTY 2 cut(s) 109, 507
AspS9I GGNCC 1 cut(s) 223
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 2 cut(s) 107, 319
AvaII GGWCC 1 cut(s) 223
AxyI CCTNAGG 1 cut(s) 120
BarI GAAGNNNNNNTAC 2 cut(s) 400, 432
BbsI GAAGAC 1 cut(s) 286
BbvI GCAGC 2 cut(s) 112, 451
BccI CCATC 2 cut(s) 159, 346
BciT130I CCWGG 2 cut(s) 12, 251
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 1 cut(s) 450
BfmI CTRYAG 2 cut(s) 126, 437
BglII AGATCT 1 cut(s) 187
BisI GCNGC 2 cut(s) 126, 440
BlsI GCNGC 2 cut(s) 127, 441
BmcAI AGTACT 2 cut(s) 361, 496
Bme1390I CCNGG 3 cut(s) 12, 251, 288
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 223
BmrFI CCNGG 3 cut(s) 12, 251, 288
BmsI GCATC 1 cut(s) 436
BpiI GAAGAC 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 250
Bse1I ACTGG 3 cut(s) 323, 431, 502
Bse21I CCTNAGG 1 cut(s) 120
BseBI CCWGG 2 cut(s) 12, 251
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 1 cut(s) 81
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 3 cut(s) 323, 431, 502
BseXI GCAGC 2 cut(s) 112, 451
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 1 cut(s) 184
BsiSI CCGG 2 cut(s) 276, 287
BsmAI GTCTC 1 cut(s) 23
BsmBI CGTCTC 1 cut(s) 23
BsnI GGCC 1 cut(s) 184
Bsp143I GATC 4 cut(s) 61, 187, 367, 394
BspACI CCGC 2 cut(s) 198, 525
BspANI GGCC 1 cut(s) 184
BspCNI CTCAG 1 cut(s) 153
BspFNI CGCG 1 cut(s) 198
BspMAI CTGCAG 2 cut(s) 130, 441
BspPI GGATC 2 cut(s) 362, 389
BsrI ACTGG 3 cut(s) 323, 431, 502
BssECI CCNNGG 1 cut(s) 250
BssMI GATC 4 cut(s) 61, 187, 367, 394
Bst2UI CCWGG 2 cut(s) 12, 251
Bst4CI ACNGT 1 cut(s) 95
Bst6I CTCTTC 3 cut(s) 120, 213, 403
BstDEI CTNAG 4 cut(s) 51, 120, 161, 179
BstF5I GGATG 1 cut(s) 81
BstFNI CGCG 1 cut(s) 198
BstKTI GATC 4 cut(s) 64, 190, 370, 397
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 4 cut(s) 61, 187, 367, 394
BstMWI GCNNNNNNNGC 1 cut(s) 522
BstNI CCWGG 2 cut(s) 12, 251
BstSCI CCNGG 3 cut(s) 10, 249, 286
BstSFI CTRYAG 2 cut(s) 126, 437
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 112, 451
BstV2I GAAGAC 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
Bsu36I CCTNAGG 1 cut(s) 120
BsuRI GGCC 1 cut(s) 184
BtsCI GGATG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 266
CaiI CAGNNNCTG 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 223
CseI GACGC 2 cut(s) 125, 459
Csp6I GTAC 3 cut(s) 360, 428, 495
CviAII CATG 5 cut(s) 45, 242, 383, 392, 490
CviJI RGCY 6 cut(s) 10, 125, 184, 238, 275, 442
CviKI_1 RGCY 6 cut(s) 10, 125, 184, 238, 275, 442
CviQI GTAC 3 cut(s) 360, 428, 495
DdeI CTNAG 4 cut(s) 51, 120, 161, 179
DpnI GATC 4 cut(s) 63, 189, 369, 396
DpnII GATC 4 cut(s) 61, 187, 367, 394
Eam1104I CTCTTC 3 cut(s) 120, 213, 403
EarI CTCTTC 3 cut(s) 120, 213, 403
Eco47I GGWCC 1 cut(s) 223
Eco81I CCTNAGG 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 223
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 2 cut(s) 10, 249
Esp3I CGTCTC 1 cut(s) 23
FaeI CATG 5 cut(s) 48, 245, 386, 395, 493
FaiI YATR 7 cut(s) 46, 243, 330, 332, 384, 393, 491
FatI CATG 5 cut(s) 44, 241, 382, 391, 489
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 2 cut(s) 126, 440
FokI GGATG 1 cut(s) 68
Fsp4HI GCNGC 2 cut(s) 126, 440
FspBI CTAG 1 cut(s) 450
GluI GCNGC 2 cut(s) 126, 440
HaeIII GGCC 1 cut(s) 184
HapII CCGG 2 cut(s) 276, 287
HgaI GACGC 2 cut(s) 125, 459
Hin1II CATG 5 cut(s) 48, 245, 386, 395, 493
HincII GTYRAC 1 cut(s) 158
HindII GTYRAC 1 cut(s) 158
HinfI GANTC 3 cut(s) 256, 485, 541
HpaII CCGG 2 cut(s) 276, 287
HphI GGTGA 2 cut(s) 107, 319
Hpy166II GTNNAC 2 cut(s) 158, 428
Hpy188I TCNGA 2 cut(s) 66, 484
Hpy188III TCNNGA 3 cut(s) 85, 365, 545
Hpy8I GTNNAC 2 cut(s) 158, 428
Hpy99I CGWCG 5 cut(s) 141, 475, 478, 481, 484
HpyAV CCTTC 2 cut(s) 92, 285
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4V TGCA 3 cut(s) 128, 245, 439
HpyF10VI GCNNNNNNNGC 1 cut(s) 522
HpyF3I CTNAG 4 cut(s) 51, 120, 161, 179
Hsp92II CATG 5 cut(s) 48, 245, 386, 395, 493
Kzo9I GATC 4 cut(s) 61, 187, 367, 394
LmnI GCTCC 1 cut(s) 41
Lsp1109I GCAGC 2 cut(s) 112, 451
LweI GCATC 1 cut(s) 436
MaeI CTAG 1 cut(s) 450
MaeIII GTNAC 1 cut(s) 264
MalI GATC 4 cut(s) 63, 189, 369, 396
MboI GATC 4 cut(s) 61, 187, 367, 394
MboII GAAGA 7 cut(s) 71, 107, 224, 230, 291, 322, 420
MflI RGATCY 1 cut(s) 187
MluCI AATT 4 cut(s) 109, 313, 341, 507
MmeI TCCRAC 2 cut(s) 86, 462
MnlI CCTC 6 cut(s) 214, 236, 298, 404, 408, 560
MseI TTAA 1 cut(s) 192
MspI CCGG 2 cut(s) 276, 287
MspR9I CCNGG 3 cut(s) 12, 251, 288
MvaI CCWGG 2 cut(s) 12, 251
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 1 cut(s) 522
NciI CCSGG 1 cut(s) 288
NdeII GATC 4 cut(s) 61, 187, 367, 394
NlaIII CATG 5 cut(s) 48, 245, 386, 395, 493
NmuCI GTSAC 1 cut(s) 264
PfeI GAWTC 3 cut(s) 256, 485, 541
PkrI GCNGC 2 cut(s) 127, 441
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
Psp6I CCWGG 2 cut(s) 10, 249
PspGI CCWGG 2 cut(s) 10, 249
PspPI GGNCC 1 cut(s) 223
PspPPI RGGWCCY 1 cut(s) 223
PstI CTGCAG 2 cut(s) 130, 441
PstNI CAGNNNCTG 1 cut(s) 70
PsuI RGATCY 1 cut(s) 187
RsaI GTAC 3 cut(s) 361, 429, 496
RsaNI GTAC 3 cut(s) 360, 428, 495
SalI GTCGAC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 2 cut(s) 126, 440
Sau3AI GATC 4 cut(s) 61, 187, 367, 394
Sau96I GGNCC 1 cut(s) 223
ScaI AGTACT 2 cut(s) 361, 496
ScrFI CCNGG 3 cut(s) 12, 251, 288
SetI ASST 4 cut(s) 103, 228, 309, 552
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 2 cut(s) 126, 437
SinI GGWCC 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
Sse9I AATT 4 cut(s) 109, 313, 341, 507
SsiI CCGC 2 cut(s) 198, 525
SspI AATATT 1 cut(s) 462
SspMI CTAG 1 cut(s) 450
StyD4I CCNGG 3 cut(s) 10, 249, 286
TaaI ACNGT 1 cut(s) 95
TaqI TCGA 2 cut(s) 157, 546
TasI AATT 4 cut(s) 109, 313, 341, 507
TatI WGTACW 2 cut(s) 359, 494
TfiI GAWTC 3 cut(s) 256, 485, 541
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 1 cut(s) 264
TseI GCWGC 2 cut(s) 125, 439
Tsp45I GTSAC 1 cut(s) 264
TspDTI ATGAA 2 cut(s) 230, 231
TspRI CASTG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 223
XapI RAATTY 2 cut(s) 109, 507
XmiI GTMKAC 1 cut(s) 157
XspI CTAG 1 cut(s) 450
ZrmI AGTACT 2 cut(s) 361, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.