Rroxscaffold_3G00274680
MYB Family

Myb-related protein 308-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001619
Physical Location & Seq
Forward (+)
66290269 .. 66291735
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274680.1

Sequence Viewer

Length: 573 bp
ATGAGGAAACCTTGCTGTGAGAAGAAGACGACCAATAAAGGAGCATGGTCGAAGCAAGAAGATGAGAAGCTCACTAAATACATTCAAAAACATGGCGAAGGAAGCTGGCGCTCTCTTCCGGTCGCTGCAGGGTTGCTTCGTTGTGGAAAGAGTTGCAGACTGAGGTGGGTAAATTATCTAAGGCCAGACCTGAAACGTGGCAACTTTGGTGAAGATGAAGAGGACCTCATCATCAAGCTCCATGCACTTCTTGGGAACAGGTGGTCACTAATTGCTGGAAGATTGCCCGGCCGGACCGACAATGAAGTGAAGAATTACTGGAATACTCATCTGAGAAGAAAGCTTATGCATTTGGGAATTGACCCCAACAACCATCGCATAGGACACAATATTTGCCTCGACAAGGTTAATCATCCATGTAAATCAGCGAATTCCCAAGCAAATAACGATGATGAAGACGAATCGCAGGATCCATTGTTGGATTCCACAAGTTCCGGCCCCGAAAGCAACACAAGTTGTACTTTGCCTGACTTGAATCTTGATCTCACGATAGGCCTTCCATGGTCACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

190

Amino Acids

21.57

Weight (kDa)

8.46

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 13 - 60 3.7e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 16 - 74 2.8e-09 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 66 - 111 1.2e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 115 1.6e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 464, 477
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 1 cut(s) 430
AfaI GTAC 1 cut(s) 520
AfiI CCNNNNNNNGG 1 cut(s) 403
AgsI TTSAA 2 cut(s) 86, 535
AluBI AGCT 4 cut(s) 70, 105, 238, 343
AluI AGCT 4 cut(s) 70, 105, 238, 343
AlwI GGATC 2 cut(s) 464, 477
AoxI GGCC 4 cut(s) 182, 289, 496, 553
ApeKI GCWGC 1 cut(s) 125
ApoI RAATTY 1 cut(s) 430
ArsI GACNNNNNNTTYG 2 cut(s) 375, 407
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 3 cut(s) 223, 294, 497
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 221
AvaII GGWCC 2 cut(s) 223, 294
BamHI GGATCC 1 cut(s) 469
BbsI GAAGAC 2 cut(s) 32, 462
BbvI GCAGC 1 cut(s) 112
BccI CCATC 1 cut(s) 381
BcnI CCSGG 1 cut(s) 288
BfmI CTRYAG 1 cut(s) 126
BfoI RGCGCY 1 cut(s) 112
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 1 cut(s) 288
Bme18I GGWCC 2 cut(s) 223, 294
BmgT120I GGNCC 3 cut(s) 223, 294, 497
BmiI GGNNCC 2 cut(s) 471, 499
BmrFI CCNGG 1 cut(s) 288
BpiI GAAGAC 2 cut(s) 32, 462
BpuMI CCSGG 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 560
BsaWI WCCGGW 1 cut(s) 118
Bsc4I CCNNNNNNNGG 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 560
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 1 cut(s) 403
BseMII CTCAG 2 cut(s) 152, 323
BseNI ACTGG 1 cut(s) 323
BseX3I CGGCCG 1 cut(s) 289
BseXI GCAGC 1 cut(s) 112
Bsh1285I CGRYCG 2 cut(s) 123, 292
BshFI GGCC 4 cut(s) 184, 291, 498, 555
BsiEI CGRYCG 2 cut(s) 123, 292
BsiSI CCGG 4 cut(s) 119, 288, 292, 495
BslI CCNNNNNNNGG 1 cut(s) 403
BsnI GGCC 4 cut(s) 184, 291, 498, 555
Bsp143I GATC 2 cut(s) 469, 541
Bsp19I CCATGG 1 cut(s) 560
BspANI GGCC 4 cut(s) 184, 291, 498, 555
BspCNI CTCAG 2 cut(s) 153, 324
BspLI GGNNCC 2 cut(s) 471, 499
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 2 cut(s) 464, 477
BsrI ACTGG 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 560
BssMI GATC 2 cut(s) 469, 541
BssT1I CCWWGG 1 cut(s) 560
Bst4CI ACNGT 1 cut(s) 570
Bst6I CTCTTC 2 cut(s) 120, 213
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 3 cut(s) 161, 179, 332
BstDSI CCRYGG 1 cut(s) 560
BstENI CCTNNNNNAGG 1 cut(s) 401
BstF5I GGATG 1 cut(s) 412
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 2 cut(s) 472, 544
BstMBI GATC 2 cut(s) 469, 541
BstMCI CGRYCG 2 cut(s) 123, 292
BstMWI GCNNNNNNNGC 2 cut(s) 102, 504
BstSCI CCNGG 1 cut(s) 286
BstSFI CTRYAG 1 cut(s) 126
BstV1I GCAGC 1 cut(s) 112
BstV2I GAAGAC 2 cut(s) 32, 462
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BstZI CGGCCG 1 cut(s) 289
BsuRI GGCC 4 cut(s) 184, 291, 498, 555
BtgI CCRYGG 1 cut(s) 560
BtgZI GCGATG 1 cut(s) 359
BtsCI GGATG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 566
Cac8I GCNNGC 1 cut(s) 107
CfoI GCGC 1 cut(s) 111
Cfr13I GGNCC 3 cut(s) 223, 294, 497
CpoI CGGWCCG 1 cut(s) 294
Csp6I GTAC 1 cut(s) 519
CspI CGGWCCG 1 cut(s) 294
CviAII CATG 5 cut(s) 45, 92, 242, 417, 561
CviJI RGCY 8 cut(s) 70, 105, 184, 238, 291, 343, 498, 555
CviKI_1 RGCY 8 cut(s) 70, 105, 184, 238, 291, 343, 498, 555
CviQI GTAC 1 cut(s) 519
DdeI CTNAG 3 cut(s) 161, 179, 332
DpnI GATC 2 cut(s) 471, 543
DpnII GATC 2 cut(s) 469, 541
EaeI YGGCCR 1 cut(s) 289
EagI CGGCCG 1 cut(s) 289
Eam1104I CTCTTC 2 cut(s) 120, 213
EarI CTCTTC 2 cut(s) 120, 213
EclXI CGGCCG 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 560
Eco147I AGGCCT 1 cut(s) 555
Eco47I GGWCC 2 cut(s) 223, 294
Eco52I CGGCCG 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 223
EcoRI GAATTC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 560
EcoT22I ATGCAT 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 560
FaeI CATG 5 cut(s) 48, 95, 245, 420, 564
FaiI YATR 7 cut(s) 46, 93, 243, 347, 380, 418, 562
FalI AAGNNNNNCTT 2 cut(s) 505, 537
FatI CATG 5 cut(s) 44, 91, 241, 416, 560
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 126
GlaI GCGC 1 cut(s) 110
GluI GCNGC 1 cut(s) 126
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 4 cut(s) 184, 291, 498, 555
HapII CCGG 4 cut(s) 119, 288, 292, 495
HhaI GCGC 1 cut(s) 111
Hin1II CATG 5 cut(s) 48, 95, 245, 420, 564
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 3 cut(s) 461, 482, 535
HpaII CCGG 4 cut(s) 119, 288, 292, 495
HphI GGTGA 1 cut(s) 221
Hpy188I TCNGA 1 cut(s) 333
Hpy188III TCNNGA 2 cut(s) 539, 547
HpyAV CCTTC 2 cut(s) 92, 566
HpyCH4III ACNGT 1 cut(s) 570
HpyCH4IV ACGT 1 cut(s) 196
HpyCH4V TGCA 4 cut(s) 128, 156, 245, 349
HpyF10VI GCNNNNNNNGC 2 cut(s) 102, 504
HpyF3I CTNAG 3 cut(s) 161, 179, 332
HpySE526I ACGT 1 cut(s) 196
Hsp92II CATG 5 cut(s) 48, 95, 245, 420, 564
HspAI GCGC 1 cut(s) 109
Kzo9I GATC 2 cut(s) 469, 541
LmnI GCTCC 2 cut(s) 41, 243
Lsp1109I GCAGC 1 cut(s) 112
MaeII ACGT 1 cut(s) 196
MaeIII GTNAC 2 cut(s) 264, 564
MalI GATC 2 cut(s) 471, 543
MboI GATC 2 cut(s) 469, 541
MflI RGATCY 1 cut(s) 469
MluCI AATT 5 cut(s) 172, 270, 313, 357, 430
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 4 cut(s) 156, 214, 236, 407
Mph1103I ATGCAT 1 cut(s) 351
MseI TTAA 1 cut(s) 408
MspI CCGG 4 cut(s) 119, 288, 292, 495
MspR9I CCNGG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 102, 504
NciI CCSGG 1 cut(s) 288
NcoI CCATGG 1 cut(s) 560
NdeII GATC 2 cut(s) 469, 541
NlaIII CATG 5 cut(s) 48, 95, 245, 420, 564
NlaIV GGNNCC 2 cut(s) 471, 499
NmuCI GTSAC 2 cut(s) 264, 564
NsiI ATGCAT 1 cut(s) 351
PceI AGGCCT 1 cut(s) 555
PfeI GAWTC 3 cut(s) 461, 482, 535
PkrI GCNGC 1 cut(s) 127
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
PspN4I GGNNCC 2 cut(s) 471, 499
PspPI GGNCC 3 cut(s) 223, 294, 497
PspPPI RGGWCCY 1 cut(s) 223
PstI CTGCAG 1 cut(s) 130
PsuI RGATCY 1 cut(s) 469
RsaI GTAC 1 cut(s) 520
RsaNI GTAC 1 cut(s) 519
Rsr2I CGGWCCG 1 cut(s) 294
RsrII CGGWCCG 1 cut(s) 294
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 2 cut(s) 469, 541
Sau96I GGNCC 3 cut(s) 223, 294, 497
ScrFI CCNGG 1 cut(s) 288
SfcI CTRYAG 1 cut(s) 126
SinI GGWCC 2 cut(s) 223, 294
Sse9I AATT 5 cut(s) 172, 270, 313, 357, 430
SseBI AGGCCT 1 cut(s) 555
SspI AATATT 1 cut(s) 391
StuI AGGCCT 1 cut(s) 555
StyD4I CCNGG 1 cut(s) 286
StyI CCWWGG 1 cut(s) 560
TaaI ACNGT 1 cut(s) 570
TaiI ACGT 1 cut(s) 199
TaqI TCGA 2 cut(s) 50, 399
TaqII GACCGA 1 cut(s) 311
TasI AATT 5 cut(s) 172, 270, 313, 357, 430
TatI WGTACW 1 cut(s) 518
TfiI GAWTC 3 cut(s) 461, 482, 535
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 1 cut(s) 573
TseFI GTSAC 2 cut(s) 264, 564
TseI GCWGC 1 cut(s) 125
Tsp45I GTSAC 2 cut(s) 264, 564
TspDTI ATGAA 3 cut(s) 231, 318, 468
TspRI CASTG 1 cut(s) 573
VpaK11BI GGWCC 2 cut(s) 223, 294
XagI CCTNNNNNAGG 1 cut(s) 401
XapI RAATTY 1 cut(s) 430
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.