pycom16g02530
MYB Family

SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001619
Physical Location & Seq
Reverse (-)
1656548 .. 1658021
1474 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g02530.1

Sequence Viewer

Length: 714 bp
ATGAGGAAACCCTGCTGCGATAAGCAGGACACGAACAAAGGAGCTTGGTCCAAGCAAGAAGACCTGAAGCTCATTGAATACATTCGTAAAAATGGCGAGGGTTGTTGGCGTACCCTTCCTCAGGCTGCAGGCCTACTTCGTTGTGGTAAAAGTTGTAGGCTGAGATGGATAAACTATCTACGACCAGACCTTAAAAGAGGCAACTTTGCTGAAGATGAAGAAGATCTCATCATCAAGCTTCATGCACTCCTAGGCAACCGGTGGTCATTGATTGCTGGGAGATTGCCAGGACGTACAGACAATGAAGTGAAAAACTACTGGAACTCTCATTTGAGAAGAAAGCTTATGAACATGGGTATAGATCCAAATAATCATCGACCGAACAACGTCAATCTCCCTCGTCTTCATCATCAAAATTCACAAACTGTCAGTAGTACTGCAACATTGTCTGCAGCTTTAAAAAACCCTACCAATTATCAGCTGCAAGCAAGATCCGGTGGTAATAATAATTGTGACCATGAATCGGACGGTACAAGTTGCTTAGAGGATGATTCTTGTGGCCGGCTGCCTGATTTAAACCTAGACCTCACAATAACTGCTCCTTTGTCTAATCCAGTTCCTGATAACCTCAAGGAGGAGCAAAATTTCGAGTCCAAAGTGTCAAAGAAGTCATCAGAATTTGCTTCATCTACCATCCTTCCTCTCTTCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

238

Amino Acids

26.85

Weight (kDa)

8.9

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 13 - 60 3.1e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 16 - 74 4.4e-10 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 66 - 111 6e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 115 1.1e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 356, 486
AcoI YGGCCR 1 cut(s) 559
AcsI RAATTY 3 cut(s) 415, 643, 677
AcuI CTGAAG 3 cut(s) 86, 231, 691
AfaI GTAC 4 cut(s) 112, 295, 436, 532
AfiI CCNNNNNNNGG 3 cut(s) 121, 523, 634
AgeI ACCGGT 1 cut(s) 258
AgsI TTSAA 1 cut(s) 77
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 6 cut(s) 44, 70, 238, 343, 455, 481
AluI AGCT 6 cut(s) 44, 70, 238, 343, 455, 481
AlwI GGATC 2 cut(s) 356, 486
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 2 cut(s) 130, 559
ApeKI GCWGC 5 cut(s) 15, 125, 452, 481, 565
ApoI RAATTY 3 cut(s) 415, 643, 677
AsiGI ACCGGT 1 cut(s) 258
Asp700I GAANNNNTTC 1 cut(s) 81
AspA2I CCTAGG 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 48
AvaII GGWCC 1 cut(s) 48
AvrII CCTAGG 1 cut(s) 250
AxyI CCTNAGG 1 cut(s) 120
BbsI GAAGAC 2 cut(s) 66, 395
BbvI GCAGC 5 cut(s) 2, 112, 464, 468, 552
BccI CCATC 2 cut(s) 159, 701
BciT130I CCWGG 1 cut(s) 288
BfaI CTAG 2 cut(s) 251, 581
BfmI CTRYAG 2 cut(s) 126, 450
BglII AGATCT 1 cut(s) 223
BisI GCNGC 5 cut(s) 16, 126, 453, 482, 566
BlnI CCTAGG 1 cut(s) 250
BlsI GCNGC 5 cut(s) 17, 127, 454, 483, 567
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 1 cut(s) 288
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 288
BpiI GAAGAC 2 cut(s) 66, 395
BpuEI CTTGAG 1 cut(s) 614
BsaJI CCNNGG 1 cut(s) 250
BsaWI WCCGGW 2 cut(s) 258, 494
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 523, 634
Bse118I RCCGGY 2 cut(s) 258, 561
Bse1I ACTGG 2 cut(s) 323, 614
Bse21I CCTNAGG 1 cut(s) 120
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 2 cut(s) 553, 693
BseLI CCNNNNNNNGG 3 cut(s) 121, 523, 634
BseMII CTCAG 2 cut(s) 134, 152
BseNI ACTGG 2 cut(s) 323, 614
BseRI GAGGAG 1 cut(s) 650
BseXI GCAGC 5 cut(s) 2, 112, 464, 468, 552
BseYI CCCAGC 1 cut(s) 275
Bsh1285I CGRYCG 1 cut(s) 380
BshFI GGCC 2 cut(s) 132, 561
BshTI ACCGGT 1 cut(s) 258
BsiEI CGRYCG 1 cut(s) 380
BsiSI CCGG 3 cut(s) 259, 495, 562
BslI CCNNNNNNNGG 3 cut(s) 121, 523, 634
BsnI GGCC 2 cut(s) 132, 561
Bsp143I GATC 3 cut(s) 223, 361, 491
BspANI GGCC 2 cut(s) 132, 561
BspCNI CTCAG 2 cut(s) 133, 153
BspMAI CTGCAG 2 cut(s) 130, 454
BspPI GGATC 2 cut(s) 356, 486
BsrFI RCCGGY 2 cut(s) 258, 561
BsrI ACTGG 2 cut(s) 323, 614
BssAI RCCGGY 2 cut(s) 258, 561
BssECI CCNNGG 1 cut(s) 250
BssMI GATC 3 cut(s) 223, 361, 491
BssT1I CCWWGG 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 2 cut(s) 427, 530
Bst6I CTCTTC 1 cut(s) 710
BstC8I GCNNGC 3 cut(s) 130, 486, 563
BstDEI CTNAG 3 cut(s) 120, 161, 541
BstENI CCTNNNNNAGG 2 cut(s) 119, 632
BstF5I GGATG 2 cut(s) 553, 693
BstKTI GATC 3 cut(s) 226, 364, 494
BstMBI GATC 3 cut(s) 223, 361, 491
BstMCI CGRYCG 1 cut(s) 380
BstNI CCWGG 1 cut(s) 288
BstSCI CCNGG 1 cut(s) 286
BstSFI CTRYAG 2 cut(s) 126, 450
BstV1I GCAGC 5 cut(s) 2, 112, 464, 468, 552
BstV2I GAAGAC 2 cut(s) 66, 395
BstX2I RGATCY 3 cut(s) 223, 361, 491
BstYI RGATCY 3 cut(s) 223, 361, 491
Bsu36I CCTNAGG 1 cut(s) 120
BsuRI GGCC 2 cut(s) 132, 561
BtsCI GGATG 2 cut(s) 553, 693
Cac8I GCNNGC 3 cut(s) 130, 486, 563
CaiI CAGNNNCTG 1 cut(s) 620
Cfr10I RCCGGY 2 cut(s) 258, 561
Cfr13I GGNCC 1 cut(s) 48
Csp6I GTAC 4 cut(s) 111, 294, 435, 531
CspAI ACCGGT 1 cut(s) 258
CviAII CATG 3 cut(s) 242, 352, 518
CviQI GTAC 4 cut(s) 111, 294, 435, 531
DdeI CTNAG 3 cut(s) 120, 161, 541
DpnI GATC 3 cut(s) 225, 363, 493
DpnII GATC 3 cut(s) 223, 361, 491
DraI TTTAAA 2 cut(s) 459, 576
EaeI YGGCCR 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 710
EarI CTCTTC 1 cut(s) 710
Eco130I CCWWGG 1 cut(s) 250
Eco147I AGGCCT 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 48
Eco57I CTGAAG 3 cut(s) 86, 231, 691
Eco81I CCTNAGG 1 cut(s) 120
EcoNI CCTNNNNNAGG 2 cut(s) 119, 632
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 3 cut(s) 245, 355, 521
FaiI YATR 5 cut(s) 243, 347, 353, 359, 519
FatI CATG 3 cut(s) 241, 351, 517
Fnu4HI GCNGC 5 cut(s) 16, 126, 453, 482, 566
FokI GGATG 2 cut(s) 560, 680
Fsp4HI GCNGC 5 cut(s) 16, 126, 453, 482, 566
FspBI CTAG 2 cut(s) 251, 581
GluI GCNGC 5 cut(s) 16, 126, 453, 482, 566
GsaI CCCAGC 1 cut(s) 279
HaeIII GGCC 2 cut(s) 132, 561
HapII CCGG 3 cut(s) 259, 495, 562
Hin1II CATG 3 cut(s) 245, 355, 521
HindIII AAGCTT 2 cut(s) 236, 341
HinfI GANTC 3 cut(s) 521, 551, 650
HpaII CCGG 3 cut(s) 259, 495, 562
Hpy188I TCNGA 3 cut(s) 526, 676, 710
Hpy188III TCNNGA 1 cut(s) 620
HpyAV CCTTC 2 cut(s) 125, 707
HpyCH4III ACNGT 2 cut(s) 427, 530
HpyCH4IV ACGT 2 cut(s) 292, 387
HpyCH4V TGCA 5 cut(s) 128, 245, 440, 452, 484
HpyF3I CTNAG 3 cut(s) 120, 161, 541
HpySE526I ACGT 2 cut(s) 292, 387
Hsp92II CATG 3 cut(s) 245, 355, 521
KroI GCCGGC 1 cut(s) 561
KroNI GCCGGC 1 cut(s) 563
Kzo9I GATC 3 cut(s) 223, 361, 491
LmnI GCTCC 3 cut(s) 41, 604, 637
Lsp1109I GCAGC 5 cut(s) 2, 112, 464, 468, 552
MaeI CTAG 2 cut(s) 251, 581
MaeII ACGT 2 cut(s) 292, 387
MaeIII GTNAC 1 cut(s) 512
MalI GATC 3 cut(s) 225, 363, 493
MboI GATC 3 cut(s) 223, 361, 491
MboII GAAGA 7 cut(s) 71, 224, 230, 233, 348, 395, 697
MflI RGATCY 3 cut(s) 223, 361, 491
MluCI AATT 5 cut(s) 415, 472, 508, 643, 677
MlyI GAGTC 1 cut(s) 659
MnlI CCTC 9 cut(s) 91, 129, 191, 408, 538, 596, 628, 638, 711
MroNI GCCGGC 1 cut(s) 561
MroXI GAANNNNTTC 1 cut(s) 81
MseI TTAA 3 cut(s) 192, 458, 575
MspA1I CMGCKG 1 cut(s) 481
MspI CCGG 3 cut(s) 259, 495, 562
MspR9I CCNGG 1 cut(s) 288
MvaI CCWGG 1 cut(s) 288
NaeI GCCGGC 1 cut(s) 563
NdeII GATC 3 cut(s) 223, 361, 491
NgoMIV GCCGGC 1 cut(s) 561
NlaIII CATG 3 cut(s) 245, 355, 521
NmuCI GTSAC 1 cut(s) 512
PceI AGGCCT 1 cut(s) 132
PdiI GCCGGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 521, 551
PinAI ACCGGT 1 cut(s) 258
PkrI GCNGC 5 cut(s) 17, 127, 454, 483, 567
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
Psp6I CCWGG 1 cut(s) 286
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 1 cut(s) 286
PspPI GGNCC 1 cut(s) 48
PstI CTGCAG 2 cut(s) 130, 454
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 3 cut(s) 223, 361, 491
PvuII CAGCTG 1 cut(s) 481
RsaI GTAC 4 cut(s) 112, 295, 436, 532
RsaNI GTAC 4 cut(s) 111, 294, 435, 531
SaqAI TTAA 3 cut(s) 192, 458, 575
SatI GCNGC 5 cut(s) 16, 126, 453, 482, 566
Sau3AI GATC 3 cut(s) 223, 361, 491
Sau96I GGNCC 1 cut(s) 48
ScaI AGTACT 1 cut(s) 436
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 1 cut(s) 288
SfcI CTRYAG 2 cut(s) 126, 450
SinI GGWCC 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 5 cut(s) 415, 472, 508, 643, 677
SseBI AGGCCT 1 cut(s) 132
SspMI CTAG 2 cut(s) 251, 581
StuI AGGCCT 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 286
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 2 cut(s) 427, 530
TaiI ACGT 2 cut(s) 295, 390
TaqI TCGA 2 cut(s) 376, 648
TaqII GACCGA 1 cut(s) 394
TasI AATT 5 cut(s) 415, 472, 508, 643, 677
TatI WGTACW 1 cut(s) 434
TfiI GAWTC 2 cut(s) 521, 551
Tru1I TTAA 3 cut(s) 192, 458, 575
Tru9I TTAA 3 cut(s) 192, 458, 575
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 5 cut(s) 15, 125, 452, 481, 565
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 7 cut(s) 230, 231, 318, 362, 395, 534, 675
VpaK11BI GGWCC 1 cut(s) 48
XagI CCTNNNNNAGG 2 cut(s) 119, 632
XapI RAATTY 3 cut(s) 415, 643, 677
XmaJI CCTAGG 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 81
XspI CTAG 2 cut(s) 251, 581
ZrmI AGTACT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.