Rw4G033000

Protein of unknown function (DUF3326)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004231
Physical Location & Seq
Forward (+)
60069805 .. 60074279
4475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033000.1

Sequence Viewer

Length: 1233 bp
ATGACGGTCCTTCTGAGCCGTCCATTCCCTTCCTTCCGGGCCGGCCAAGACCTCCGTATCTTCAAATCCAGACCGTCCATTAAAACAAGCGTCTCATGCTTCACTCCCAAACACTCCTTCAAGAGAGACTACACTAGCGTGATGATAGTCCCGACCGGAGTCGGAGCCGCCATAGGTGGATACGCCGGCGACGCCTTGCCGGTGGCTCGCACTCTCGCCTCCGTCGTCGATTGTCTCATCACTCATCCCAATGTGCTGAATGCAGCAATGCTCTGTTGGCCCATGCCTAATGCTTTGTATGTCGAAGGTTATGCTCTCGACCGATTCGCACAAGGCTTATGGGCTCTGAAGCCTGTTCACCAGAACAAGGTGGGGTTGGTTCTTGATTGTGGAATGGAAGAAGAGCTTCGCGTTCGTCATTTGCAAGTGGCTGATGCTGCAAGGGCTTCCCTTGGCTTGCCTGTAGTGGAATATGTTGTCACTGACACTCCTTTGCAGGTAGAGAAGTGGATTGATACAAAAAGTGGGCAATCAACGGGGAGCATTAAGCACCCTGAATCACTACTGAGGGCTGTGCAGGCTTTAATCAAACGCTCTCAAGTAAATGCCGTTGCAGTTGTTGGACGTTTCCCGGATGATGATTTTGAAGACACTGATGATTATCGACAAGGGATGGGAATTGATCTATTGGCAGGAGTTGAGGCAGTTATAAGCCATCTAGTAGTGAAAGAATTCCAAATTCCTTGTGCACATGCTCCTGCTTTATTACCTGTATCTCTGAGCCCATCTTTATGCCCAAAATCAGCTGCTGAGGAGTTGGGATACACATTCTTGCCTTGTGTGCTTGCTGGCCTTAGTAATGCACCACAATACTTGGTCAAGAACTCTGAGTCCGAGCATGGTTGCATACTGGCAAATGATGTTGATAGTGTCATCCTTCCTATAGATGCTTGTGGAGGACCTGGTGCTCTTGCTTTTGCACAAAGCAAAGCCAATAAGCCCCTTATAATTGCTGTGGAGGAAAATGAAACAGTCCTTGATGACACACCTGATAAAGTTGGCATTGAAGCAATCCATGTCTCAAACTATTGGGAAGCGATTGGTGTTGTTGCAGCTCACAAGGCTGGAGTAAATCCGCAGTCACTACGAAGAAATAGAATTAGCAGTCTTCAACGCGCGTCTCTATTGCCTGCTAATGGTTATGCCGTTCAAAGTGCCACAACAAATGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3326

Protein Analysis

410

Amino Acids

43.91

Weight (kDa)

5.94

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3326 PF11805 45 - 383 1.2e-159 Protein of unknown function (DUF3326)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 710, 1007
Acc36I ACCTGC 1 cut(s) 487
AccII CGCG 3 cut(s) 411, 1176, 1178
AciI CCGC 2 cut(s) 168, 1136
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 2 cut(s) 731, 738
AcuI CTGAAG 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 192
AfiI CCNNNNNNNGG 2 cut(s) 367, 1196
AgsI TTSAA 6 cut(s) 64, 121, 647, 1067, 1172, 1211
AjnI CCWGG 1 cut(s) 961
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AleI CACNNNNGTG 1 cut(s) 137
AloI GAACNNNNNNTCC 2 cut(s) 875, 907
AluBI AGCT 3 cut(s) 406, 806, 1115
AluI AGCT 3 cut(s) 406, 806, 1115
Alw21I GWGCWC 2 cut(s) 751, 970
Alw26I GTCTC 5 cut(s) 97, 120, 239, 1084, 1185
Alw44I GTGCAC 1 cut(s) 747
AlwNI CAGNNNCTG 1 cut(s) 809
AoxI GGCC 4 cut(s) 39, 43, 278, 850
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 4 cut(s) 263, 437, 806, 1112
ApoI RAATTY 2 cut(s) 731, 738
Asp700I GAANNNNTTC 2 cut(s) 405, 731
AspLEI GCGC 1 cut(s) 1178
AspS9I GGNCC 4 cut(s) 7, 39, 279, 959
AsuC2I CCSGG 2 cut(s) 38, 632
AsuHPI GGTGA 1 cut(s) 350
AvaII GGWCC 2 cut(s) 7, 959
BaeGI GKGCMC 1 cut(s) 751
BaeI ACNNNNGTAYC 2 cut(s) 40, 73
BanII GRGCYC 2 cut(s) 346, 785
BbsI GAAGAC 2 cut(s) 654, 1160
Bbv12I GWGCWC 2 cut(s) 751, 970
BbvCI CCTCAGC 1 cut(s) 810
BbvI GCAGC 4 cut(s) 275, 424, 793, 1124
BccI CCATC 3 cut(s) 667, 723, 793
BceAI ACGGC 3 cut(s) 3, 593, 1190
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 1 cut(s) 963
BciVI GTATCC 2 cut(s) 173, 815
BcnI CCSGG 2 cut(s) 38, 632
BcoDI GTCTC 5 cut(s) 97, 120, 239, 1084, 1185
BfaI CTAG 2 cut(s) 135, 719
BfmI CTRYAG 2 cut(s) 462, 942
BfuAI ACCTGC 1 cut(s) 487
BfuI GTATCC 2 cut(s) 173, 815
BisI GCNGC 5 cut(s) 168, 264, 438, 807, 1113
BlsI GCNGC 5 cut(s) 169, 265, 439, 808, 1114
Bme1390I CCNGG 3 cut(s) 38, 632, 963
Bme18I GGWCC 2 cut(s) 7, 959
BmgT120I GGNCC 4 cut(s) 7, 39, 279, 959
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 3 cut(s) 38, 632, 963
BmsI GCATC 2 cut(s) 424, 937
BoxI GACNNNNGTC 1 cut(s) 158
BpiI GAAGAC 2 cut(s) 654, 1160
BpmI CTGGAG 1 cut(s) 1146
Bpu10I CCTNAGC 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 582
BpuMI CCSGG 2 cut(s) 38, 632
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 472, 502
Bsc4I CCNNNNNNNGG 2 cut(s) 367, 1196
Bse118I RCCGGY 3 cut(s) 41, 185, 199
Bse1I ACTGG 1 cut(s) 915
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 1 cut(s) 963
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 4 cut(s) 244, 640, 678, 933
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 2 cut(s) 367, 1196
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 5 cut(s) 5, 557, 770, 801, 879
BseNI ACTGG 1 cut(s) 915
BseRI GAGGAG 1 cut(s) 827
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 4 cut(s) 275, 424, 793, 1124
BsgI GTGCAG 1 cut(s) 596
Bsh1236I CGCG 3 cut(s) 411, 1176, 1178
Bsh1285I CGRYCG 2 cut(s) 156, 322
BshFI GGCC 4 cut(s) 41, 45, 280, 852
BsiEI CGRYCG 2 cut(s) 156, 322
BsiHKAI GWGCWC 2 cut(s) 751, 970
BsiSI CCGG 6 cut(s) 37, 42, 156, 186, 200, 632
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 2 cut(s) 367, 1196
BsmAI GTCTC 5 cut(s) 97, 120, 239, 1084, 1185
BsmBI CGTCTC 2 cut(s) 97, 1185
BsmFI GGGAC 1 cut(s) 134
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 4 cut(s) 41, 45, 280, 852
Bsp1286I GDGCHC 4 cut(s) 346, 751, 785, 970
Bsp143I GATC 1 cut(s) 682
BspACI CCGC 2 cut(s) 168, 1136
BspANI GGCC 4 cut(s) 41, 45, 280, 852
BspCNI CTCAG 5 cut(s) 6, 558, 771, 802, 880
BspFNI CGCG 3 cut(s) 411, 1176, 1178
BspLI GGNNCC 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 487
BspQI GCTCTTC 1 cut(s) 396
BsrDI GCAATG 1 cut(s) 273
BsrFI RCCGGY 3 cut(s) 41, 185, 199
BsrI ACTGG 1 cut(s) 915
BssAI RCCGGY 3 cut(s) 41, 185, 199
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 1 cut(s) 682
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 1 cut(s) 963
Bst4CI ACNGT 3 cut(s) 7, 75, 1033
Bst6I CTCTTC 1 cut(s) 396
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 8 cut(s) 43, 187, 208, 458, 579, 846, 850, 1191
BstDEI CTNAG 6 cut(s) 14, 566, 779, 810, 854, 888
BstF5I GGATG 4 cut(s) 244, 640, 678, 933
BstFNI CGCG 3 cut(s) 411, 1176, 1178
BstHHI GCGC 1 cut(s) 1178
BstKTI GATC 1 cut(s) 685
BstMAI GTCTC 5 cut(s) 97, 120, 239, 1084, 1185
BstMBI GATC 1 cut(s) 682
BstMCI CGRYCG 2 cut(s) 156, 322
BstMWI GCNNNNNNNGC 8 cut(s) 96, 191, 277, 437, 443, 578, 841, 1121
BstNI CCWGG 1 cut(s) 963
BstNSI RCATGY 1 cut(s) 755
BstPAI GACNNNNGTC 1 cut(s) 158
BstSCI CCNGG 3 cut(s) 36, 630, 961
BstSFI CTRYAG 2 cut(s) 462, 942
BstSLI GKGCMC 1 cut(s) 751
BstUI CGCG 3 cut(s) 411, 1176, 1178
BstV1I GCAGC 4 cut(s) 275, 424, 793, 1124
BstV2I GAAGAC 2 cut(s) 654, 1160
BsuI GTATCC 2 cut(s) 173, 815
BsuRI GGCC 4 cut(s) 41, 45, 280, 852
BtsCI GGATG 4 cut(s) 244, 640, 678, 933
BtsIMutI CAGTG 2 cut(s) 480, 651
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 8 cut(s) 43, 187, 208, 458, 579, 846, 850, 1191
CaiI CAGNNNCTG 1 cut(s) 809
CfoI GCGC 1 cut(s) 1178
Cfr10I RCCGGY 3 cut(s) 41, 185, 199
Cfr13I GGNCC 4 cut(s) 7, 39, 279, 959
CseI GACGC 3 cut(s) 79, 200, 1167
CsiI ACCWGGT 1 cut(s) 961
CspCI CAANNNNNGTGG 2 cut(s) 855, 890
CviAII CATG 5 cut(s) 96, 283, 752, 899, 1076
DdeI CTNAG 6 cut(s) 14, 566, 779, 810, 854, 888
DpnI GATC 1 cut(s) 684
DpnII GATC 1 cut(s) 682
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 2 cut(s) 346, 785
Eco47I GGWCC 2 cut(s) 7, 959
Eco57I CTGAAG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 959
EcoRI GAATTC 1 cut(s) 731
EcoRII CCWGG 1 cut(s) 961
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 2 cut(s) 346, 785
ErhI CCWWGG 1 cut(s) 451
Esp3I CGTCTC 2 cut(s) 97, 1185
FaeI CATG 5 cut(s) 99, 286, 755, 902, 1079
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 1 cut(s) 134
FatI CATG 5 cut(s) 95, 282, 751, 898, 1075
Fnu4HI GCNGC 5 cut(s) 168, 264, 438, 807, 1113
FokI GGATG 4 cut(s) 231, 647, 685, 920
FriOI GRGCYC 2 cut(s) 346, 785
FseI GGCCGGCC 1 cut(s) 45
Fsp4HI GCNGC 5 cut(s) 168, 264, 438, 807, 1113
FspBI CTAG 2 cut(s) 135, 719
GlaI GCGC 1 cut(s) 1177
GluI GCNGC 5 cut(s) 168, 264, 438, 807, 1113
GsuI CTGGAG 1 cut(s) 1146
HaeIII GGCC 4 cut(s) 41, 45, 280, 852
HapII CCGG 6 cut(s) 37, 42, 156, 186, 200, 632
HgaI GACGC 3 cut(s) 79, 200, 1167
HhaI GCGC 1 cut(s) 1178
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 5 cut(s) 99, 286, 755, 902, 1079
Hin6I GCGC 1 cut(s) 1176
HinP1I GCGC 1 cut(s) 1176
HinfI GANTC 4 cut(s) 159, 324, 557, 890
HpaII CCGG 6 cut(s) 37, 42, 156, 186, 200, 632
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 2 cut(s) 358, 749
Hpy188I TCNGA 6 cut(s) 15, 164, 348, 780, 889, 895
Hpy188III TCNNGA 6 cut(s) 69, 121, 151, 317, 383, 880
Hpy8I GTNNAC 2 cut(s) 358, 749
Hpy99I CGWCG 3 cut(s) 194, 227, 230
HpyAV CCTTC 6 cut(s) 20, 39, 43, 127, 299, 947
HpyCH4III ACNGT 3 cut(s) 7, 75, 1033
HpyCH4IV ACGT 1 cut(s) 625
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 191, 277, 437, 443, 578, 841, 1121
HpyF3I CTNAG 6 cut(s) 14, 566, 779, 810, 854, 888
HpySE526I ACGT 1 cut(s) 625
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 5 cut(s) 99, 286, 755, 902, 1079
HspAI GCGC 1 cut(s) 1176
KroI GCCGGC 2 cut(s) 41, 185
KroNI GCCGGC 2 cut(s) 43, 187
Kzo9I GATC 1 cut(s) 682
LguI GCTCTTC 1 cut(s) 396
LmnI GCTCC 3 cut(s) 164, 540, 760
Lsp1109I GCAGC 4 cut(s) 275, 424, 793, 1124
LweI GCATC 2 cut(s) 424, 937
MabI ACCWGGT 1 cut(s) 961
MaeI CTAG 2 cut(s) 135, 719
MaeII ACGT 1 cut(s) 625
MaeIII GTNAC 2 cut(s) 478, 1140
MalI GATC 1 cut(s) 684
MboI GATC 1 cut(s) 682
MboII GAAGA 6 cut(s) 52, 410, 413, 659, 1160, 1161
MhlI GDGCHC 4 cut(s) 346, 751, 785, 970
MluCI AATT 5 cut(s) 678, 731, 738, 1008, 1158
MlyI GAGTC 2 cut(s) 168, 899
MmeI TCCRAC 2 cut(s) 142, 601
MnlI CCTC 7 cut(s) 62, 229, 561, 694, 805, 950, 1012
MreI CGCCGGCG 1 cut(s) 185
MroNI GCCGGC 2 cut(s) 41, 185
MroXI GAANNNNTTC 2 cut(s) 405, 731
MseI TTAA 3 cut(s) 81, 546, 584
MslI CAYNNNNRTG 3 cut(s) 137, 249, 790
MspA1I CMGCKG 1 cut(s) 806
MspI CCGG 6 cut(s) 37, 42, 156, 186, 200, 632
MspR9I CCNGG 3 cut(s) 38, 632, 963
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 1 cut(s) 963
MvnI CGCG 3 cut(s) 411, 1176, 1178
MwoI GCNNNNNNNGC 8 cut(s) 96, 191, 277, 437, 443, 578, 841, 1121
NaeI GCCGGC 2 cut(s) 43, 187
NciI CCSGG 2 cut(s) 38, 632
NdeII GATC 1 cut(s) 682
NgoMIV GCCGGC 2 cut(s) 41, 185
NlaIII CATG 5 cut(s) 99, 286, 755, 902, 1079
NlaIV GGNNCC 1 cut(s) 166
NmuCI GTSAC 2 cut(s) 478, 1140
NspI RCATGY 1 cut(s) 755
OliI CACNNNNGTG 1 cut(s) 137
PciSI GCTCTTC 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 265
PdiI GCCGGC 2 cut(s) 43, 187
PdmI GAANNNNTTC 2 cut(s) 405, 731
PfeI GAWTC 2 cut(s) 324, 557
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 5 cut(s) 169, 265, 439, 808, 1114
PleI GAGTC 2 cut(s) 167, 898
PpsI GAGTC 2 cut(s) 167, 898
PpuMI RGGWCCY 1 cut(s) 959
PshAI GACNNNNGTC 1 cut(s) 158
PsiI TTATAA 2 cut(s) 710, 1007
Psp5II RGGWCCY 1 cut(s) 959
Psp6I CCWGG 1 cut(s) 961
PspGI CCWGG 1 cut(s) 961
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 4 cut(s) 7, 39, 279, 959
PspPPI RGGWCCY 1 cut(s) 959
PstNI CAGNNNCTG 1 cut(s) 809
PvuII CAGCTG 1 cut(s) 806
RigI GGCCGGCC 1 cut(s) 45
RseI CAYNNNNRTG 3 cut(s) 137, 249, 790
SapI GCTCTTC 1 cut(s) 396
SaqAI TTAA 3 cut(s) 81, 546, 584
SatI GCNGC 5 cut(s) 168, 264, 438, 807, 1113
Sau3AI GATC 1 cut(s) 682
Sau96I GGNCC 4 cut(s) 7, 39, 279, 959
SchI GAGTC 2 cut(s) 168, 899
ScrFI CCNGG 3 cut(s) 38, 632, 963
SduI GDGCHC 4 cut(s) 346, 751, 785, 970
SexAI ACCWGGT 1 cut(s) 961
SfaNI GCATC 2 cut(s) 424, 937
SfcI CTRYAG 2 cut(s) 462, 942
SgrAI CRCCGGYG 1 cut(s) 185
SinI GGWCC 2 cut(s) 7, 959
SmiMI CAYNNNNRTG 3 cut(s) 137, 249, 790
SmlI CTYRAG 1 cut(s) 597
SmoI CTYRAG 1 cut(s) 597
Sse9I AATT 5 cut(s) 678, 731, 738, 1008, 1158
SsiI CCGC 2 cut(s) 168, 1136
SspMI CTAG 2 cut(s) 135, 719
StyD4I CCNGG 3 cut(s) 36, 630, 961
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 3 cut(s) 7, 75, 1033
TaiI ACGT 1 cut(s) 628
TaqI TCGA 4 cut(s) 228, 303, 318, 664
TaqII GACCGA 1 cut(s) 336
TasI AATT 5 cut(s) 678, 731, 738, 1008, 1158
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 2 cut(s) 324, 557
Tru1I TTAA 3 cut(s) 81, 546, 584
Tru9I TTAA 3 cut(s) 81, 546, 584
TscAI CASTG 2 cut(s) 487, 658
TseFI GTSAC 2 cut(s) 478, 1140
TseI GCWGC 4 cut(s) 263, 437, 806, 1112
Tsp45I GTSAC 2 cut(s) 478, 1140
TspDTI ATGAA 1 cut(s) 1041
TspGWI ACGGA 2 cut(s) 44, 211
TspRI CASTG 2 cut(s) 487, 658
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 2 cut(s) 7, 959
XapI RAATTY 2 cut(s) 731, 738
XceI RCATGY 1 cut(s) 755
XmnI GAANNNNTTC 2 cut(s) 405, 731
XspI CTAG 2 cut(s) 135, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.