Rh4BG395900

Protein of unknown function (DUF3326)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004231
Physical Location & Seq
Forward (+)
54738710 .. 54743928
5219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG395900.1

Sequence Viewer

Length: 1011 bp
ATGACGGTCCTTCTGAGCCGTCCATTCCCTTCCTTCCGGGCCGGCCAAGACCTCCGTATCTTCAAATCCAGACCGTCCATTAAAACAAGCGTCTCATGCTTCACTCCCAAACACTCCTTCAAGAGAGACTACACTAGCGTGATGATAGTCCCCACCGGAGTCGGAGCCGCCATAGGTGGATACGCCGGCGACGCCTTGCCGGTGGCTCGCACTCTCGCCTCCGTCGTCGATTGTCTCATCACTCATCCCAATGTGCTGAATGCAGCAATGCTCTGTTGGCCCATGCCTAATGCTTTGTATGTCGAAGGTTATGCTCTCGACCGATTCGCAGAAGGCTCATGGGCTCTGAAGCCTGTTCACCAGAACAAGGTGGGGTTGGTTCTTGATTGTGGAATGGAAGAAGAGCTTCGCGTTCGTCATTTGCAAGTGGCTGATGCTGCAAGGGCTTCCCTTGGCTTGCCTGTAGTGGAATATGTTGTCACTGACACTCCTTTGCAGGTAGAGAAGTGGATTGATACAAAAAGTGGGCAATCAACGGGGAGCATTAAGCACCCTGAATCACTACTGAGGGCTGTGCAGGCTTTAATCAAACGCTCTCAAGTAAATGCCGTTGCAGTTGTTGGACGTTTCCCGGATGGTGATTTTGAAGACACTGATGATTATCGACAAGGGATGGGAATTGATCTATTGGCAGGAGTTGAGGCAGTTATAAGCCATCTAGTAGTGAAAGAATTCCAAATTCCTTGTGCACATGCTCCTGCTTTATTACCTGTACCTCTGTGCCCATCTTTATGCCCAAAATCAGCTGCTGAGGAGTTGGGATACACATTCTTGCCTTGTGTGCTTGCTGGCCTTAGTAATGCACCACAATACTTGGTCAAGTACTCTGAGTCCGAGCATGGTTGCATACTGGCAAATGATGTTGATAGTGTCATCCTTCCTATAGATGCTTGTGGAGGATCTGGTGCTCTTGCTTTTGCACAAAGCAAAGCCAATAAGGTACACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3326

Protein Analysis

336

Amino Acids

36.05

Weight (kDa)

6.14

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3326 PF11805 45 - 331 6.3e-139 Protein of unknown function (DUF3326)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
Acc36I ACCTGC 1 cut(s) 487
AccII CGCG 1 cut(s) 411
AciI CCGC 1 cut(s) 168
AclWI GGATC 1 cut(s) 967
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 2 cut(s) 731, 738
AcuI CTGAAG 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 3 cut(s) 774, 884, 1002
AfiI CCNNNNNNNGG 1 cut(s) 367
AgsI TTSAA 3 cut(s) 64, 121, 647
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 2 cut(s) 406, 806
AluI AGCT 2 cut(s) 406, 806
Alw21I GWGCWC 2 cut(s) 751, 970
Alw26I GTCTC 3 cut(s) 97, 120, 239
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 1 cut(s) 967
AlwNI CAGNNNCTG 1 cut(s) 809
AoxI GGCC 4 cut(s) 39, 43, 278, 850
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 3 cut(s) 263, 437, 806
ApoI RAATTY 2 cut(s) 731, 738
Asp700I GAANNNNTTC 2 cut(s) 405, 731
AspS9I GGNCC 3 cut(s) 7, 39, 279
AsuC2I CCSGG 2 cut(s) 38, 632
AsuHPI GGTGA 2 cut(s) 350, 650
AvaII GGWCC 1 cut(s) 7
BaeGI GKGCMC 2 cut(s) 751, 785
BaeI ACNNNNGTAYC 2 cut(s) 40, 73
BanII GRGCYC 1 cut(s) 346
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 2 cut(s) 751, 970
BbvCI CCTCAGC 1 cut(s) 810
BbvI GCAGC 3 cut(s) 275, 424, 793
BccI CCATC 4 cut(s) 629, 667, 723, 793
BceAI ACGGC 2 cut(s) 3, 593
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciVI GTATCC 2 cut(s) 173, 815
BcnI CCSGG 2 cut(s) 38, 632
BcoDI GTCTC 3 cut(s) 97, 120, 239
BfaI CTAG 2 cut(s) 135, 719
BfmI CTRYAG 2 cut(s) 462, 942
BfuAI ACCTGC 1 cut(s) 487
BfuI GTATCC 2 cut(s) 173, 815
BisI GCNGC 4 cut(s) 168, 264, 438, 807
BlsI GCNGC 4 cut(s) 169, 265, 439, 808
BmcAI AGTACT 1 cut(s) 884
Bme1390I CCNGG 2 cut(s) 38, 632
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 3 cut(s) 7, 39, 279
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 2 cut(s) 38, 632
BmsI GCATC 2 cut(s) 424, 937
BpiI GAAGAC 1 cut(s) 654
Bpu10I CCTNAGC 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 582
BpuMI CCSGG 2 cut(s) 38, 632
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 472, 502
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse118I RCCGGY 3 cut(s) 41, 185, 199
Bse1I ACTGG 1 cut(s) 915
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 660
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 4 cut(s) 244, 640, 678, 933
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 4 cut(s) 5, 557, 801, 879
BseNI ACTGG 1 cut(s) 915
BseRI GAGGAG 1 cut(s) 827
BseSI GKGCMC 2 cut(s) 751, 785
BseXI GCAGC 3 cut(s) 275, 424, 793
BsgI GTGCAG 1 cut(s) 596
Bsh1236I CGCG 1 cut(s) 411
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 4 cut(s) 41, 45, 280, 852
BsiEI CGRYCG 1 cut(s) 322
BsiHKAI GWGCWC 2 cut(s) 751, 970
BsiSI CCGG 6 cut(s) 37, 42, 156, 186, 200, 632
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 3 cut(s) 97, 120, 239
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 134
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 4 cut(s) 41, 45, 280, 852
Bsp1286I GDGCHC 4 cut(s) 346, 751, 785, 970
Bsp143I GATC 2 cut(s) 682, 959
BspACI CCGC 1 cut(s) 168
BspANI GGCC 4 cut(s) 41, 45, 280, 852
BspCNI CTCAG 4 cut(s) 6, 558, 802, 880
BspFNI CGCG 1 cut(s) 411
BspLI GGNNCC 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 1 cut(s) 967
BspQI GCTCTTC 1 cut(s) 396
BsrDI GCAATG 1 cut(s) 273
BsrFI RCCGGY 3 cut(s) 41, 185, 199
BsrI ACTGG 1 cut(s) 915
BssAI RCCGGY 3 cut(s) 41, 185, 199
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 2 cut(s) 682, 959
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 451
Bst4CI ACNGT 2 cut(s) 7, 75
Bst6I CTCTTC 1 cut(s) 396
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 7 cut(s) 43, 187, 208, 458, 579, 846, 850
BstDEI CTNAG 5 cut(s) 14, 566, 810, 854, 888
BstF5I GGATG 4 cut(s) 244, 640, 678, 933
BstFNI CGCG 1 cut(s) 411
BstKTI GATC 2 cut(s) 685, 962
BstMAI GTCTC 3 cut(s) 97, 120, 239
BstMBI GATC 2 cut(s) 682, 959
BstMCI CGRYCG 1 cut(s) 322
BstMWI GCNNNNNNNGC 7 cut(s) 96, 191, 277, 437, 443, 578, 841
BstNSI RCATGY 1 cut(s) 755
BstSCI CCNGG 2 cut(s) 36, 630
BstSFI CTRYAG 2 cut(s) 462, 942
BstSLI GKGCMC 2 cut(s) 751, 785
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 3 cut(s) 275, 424, 793
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 1 cut(s) 959
BstYI RGATCY 1 cut(s) 959
BsuI GTATCC 2 cut(s) 173, 815
BsuRI GGCC 4 cut(s) 41, 45, 280, 852
BtsCI GGATG 4 cut(s) 244, 640, 678, 933
BtsIMutI CAGTG 2 cut(s) 480, 651
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 7 cut(s) 43, 187, 208, 458, 579, 846, 850
CaiI CAGNNNCTG 1 cut(s) 809
Cfr10I RCCGGY 3 cut(s) 41, 185, 199
Cfr13I GGNCC 3 cut(s) 7, 39, 279
CseI GACGC 2 cut(s) 79, 200
Csp6I GTAC 3 cut(s) 773, 883, 1001
CspCI CAANNNNNGTGG 2 cut(s) 855, 890
CviAII CATG 5 cut(s) 96, 283, 339, 752, 899
CviQI GTAC 3 cut(s) 773, 883, 1001
DdeI CTNAG 5 cut(s) 14, 566, 810, 854, 888
DpnI GATC 2 cut(s) 684, 961
DpnII GATC 2 cut(s) 682, 959
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 731
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 451
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 5 cut(s) 99, 286, 342, 755, 902
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 1 cut(s) 134
FatI CATG 5 cut(s) 95, 282, 338, 751, 898
Fnu4HI GCNGC 4 cut(s) 168, 264, 438, 807
FokI GGATG 4 cut(s) 231, 647, 685, 920
FriOI GRGCYC 1 cut(s) 346
FseI GGCCGGCC 1 cut(s) 45
Fsp4HI GCNGC 4 cut(s) 168, 264, 438, 807
FspBI CTAG 2 cut(s) 135, 719
GluI GCNGC 4 cut(s) 168, 264, 438, 807
HaeIII GGCC 4 cut(s) 41, 45, 280, 852
HapII CCGG 6 cut(s) 37, 42, 156, 186, 200, 632
HgaI GACGC 2 cut(s) 79, 200
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 5 cut(s) 99, 286, 342, 755, 902
HinfI GANTC 4 cut(s) 159, 324, 557, 890
HpaII CCGG 6 cut(s) 37, 42, 156, 186, 200, 632
HphI GGTGA 2 cut(s) 350, 650
Hpy166II GTNNAC 3 cut(s) 358, 749, 1003
Hpy188I TCNGA 5 cut(s) 15, 164, 348, 889, 895
Hpy188III TCNNGA 4 cut(s) 69, 121, 317, 383
Hpy8I GTNNAC 3 cut(s) 358, 749, 1003
Hpy99I CGWCG 3 cut(s) 194, 227, 230
HpyAV CCTTC 7 cut(s) 20, 39, 43, 127, 299, 326, 947
HpyCH4III ACNGT 2 cut(s) 7, 75
HpyCH4IV ACGT 1 cut(s) 625
HpyF10VI GCNNNNNNNGC 7 cut(s) 96, 191, 277, 437, 443, 578, 841
HpyF3I CTNAG 5 cut(s) 14, 566, 810, 854, 888
HpySE526I ACGT 1 cut(s) 625
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 5 cut(s) 99, 286, 342, 755, 902
KroI GCCGGC 2 cut(s) 41, 185
KroNI GCCGGC 2 cut(s) 43, 187
Kzo9I GATC 2 cut(s) 682, 959
LguI GCTCTTC 1 cut(s) 396
LmnI GCTCC 3 cut(s) 164, 540, 760
Lsp1109I GCAGC 3 cut(s) 275, 424, 793
LweI GCATC 2 cut(s) 424, 937
MaeI CTAG 2 cut(s) 135, 719
MaeII ACGT 1 cut(s) 625
MaeIII GTNAC 1 cut(s) 478
MalI GATC 2 cut(s) 684, 961
MboI GATC 2 cut(s) 682, 959
MboII GAAGA 4 cut(s) 52, 410, 413, 659
MfeI CAATTG 1 cut(s) 1006
MflI RGATCY 1 cut(s) 959
MhlI GDGCHC 4 cut(s) 346, 751, 785, 970
MluCI AATT 4 cut(s) 678, 731, 738, 1006
MlyI GAGTC 2 cut(s) 168, 899
MmeI TCCRAC 2 cut(s) 142, 601
MnlI CCTC 7 cut(s) 62, 229, 561, 694, 786, 805, 950
MreI CGCCGGCG 1 cut(s) 185
MroNI GCCGGC 2 cut(s) 41, 185
MroXI GAANNNNTTC 2 cut(s) 405, 731
MseI TTAA 3 cut(s) 81, 546, 584
MslI CAYNNNNRTG 3 cut(s) 137, 249, 790
MspA1I CMGCKG 1 cut(s) 806
MspI CCGG 6 cut(s) 37, 42, 156, 186, 200, 632
MspR9I CCNGG 2 cut(s) 38, 632
MunI CAATTG 1 cut(s) 1006
Mva1269I GAATGC 1 cut(s) 265
MvnI CGCG 1 cut(s) 411
MwoI GCNNNNNNNGC 7 cut(s) 96, 191, 277, 437, 443, 578, 841
NaeI GCCGGC 2 cut(s) 43, 187
NciI CCSGG 2 cut(s) 38, 632
NdeII GATC 2 cut(s) 682, 959
NgoMIV GCCGGC 2 cut(s) 41, 185
NlaIII CATG 5 cut(s) 99, 286, 342, 755, 902
NlaIV GGNNCC 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 478
NspI RCATGY 1 cut(s) 755
OliI CACNNNNGTG 1 cut(s) 137
PciSI GCTCTTC 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 265
PdiI GCCGGC 2 cut(s) 43, 187
PdmI GAANNNNTTC 2 cut(s) 405, 731
PfeI GAWTC 2 cut(s) 324, 557
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 4 cut(s) 169, 265, 439, 808
PleI GAGTC 2 cut(s) 167, 898
PpsI GAGTC 2 cut(s) 167, 898
PsiI TTATAA 1 cut(s) 710
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 3 cut(s) 7, 39, 279
PstNI CAGNNNCTG 1 cut(s) 809
PsuI RGATCY 1 cut(s) 959
PvuII CAGCTG 1 cut(s) 806
RigI GGCCGGCC 1 cut(s) 45
RsaI GTAC 3 cut(s) 774, 884, 1002
RsaNI GTAC 3 cut(s) 773, 883, 1001
RseI CAYNNNNRTG 3 cut(s) 137, 249, 790
SapI GCTCTTC 1 cut(s) 396
SaqAI TTAA 3 cut(s) 81, 546, 584
SatI GCNGC 4 cut(s) 168, 264, 438, 807
Sau3AI GATC 2 cut(s) 682, 959
Sau96I GGNCC 3 cut(s) 7, 39, 279
ScaI AGTACT 1 cut(s) 884
SchI GAGTC 2 cut(s) 168, 899
ScrFI CCNGG 2 cut(s) 38, 632
SduI GDGCHC 4 cut(s) 346, 751, 785, 970
SfaNI GCATC 2 cut(s) 424, 937
SfcI CTRYAG 2 cut(s) 462, 942
SgrAI CRCCGGYG 1 cut(s) 185
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 3 cut(s) 137, 249, 790
SmlI CTYRAG 1 cut(s) 597
SmoI CTYRAG 1 cut(s) 597
Sse9I AATT 4 cut(s) 678, 731, 738, 1006
SsiI CCGC 1 cut(s) 168
SspMI CTAG 2 cut(s) 135, 719
StyD4I CCNGG 2 cut(s) 36, 630
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 2 cut(s) 7, 75
TaiI ACGT 1 cut(s) 628
TaqI TCGA 4 cut(s) 228, 303, 318, 664
TaqII GACCGA 1 cut(s) 336
TasI AATT 4 cut(s) 678, 731, 738, 1006
TatI WGTACW 1 cut(s) 882
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 2 cut(s) 324, 557
Tru1I TTAA 3 cut(s) 81, 546, 584
Tru9I TTAA 3 cut(s) 81, 546, 584
TscAI CASTG 2 cut(s) 487, 658
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 3 cut(s) 263, 437, 806
Tsp45I GTSAC 1 cut(s) 478
TspGWI ACGGA 2 cut(s) 44, 211
TspRI CASTG 2 cut(s) 487, 658
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 2 cut(s) 731, 738
XceI RCATGY 1 cut(s) 755
XmnI GAANNNNTTC 2 cut(s) 405, 731
XspI CTAG 2 cut(s) 135, 719
ZrmI AGTACT 1 cut(s) 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.