RLG00000006120

Protein of unknown function (DUF3326)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004231
Physical Location & Seq
Reverse (-)
4551349 .. 4556689
5341 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006120

Sequence Viewer

Length: 1233 bp
ATGACGGTCCTTCTGAGCCGTCCATTCCCTTCCTTCCGGGCCGGCCAAGACCTCCGTATCTTCAAATCCAGACCGTCCATTAAAACAAGCGTCTCATGCTTCACTCCCAAACACTCCTTCAAGAGAGACTACACTAGCGTGATGATAGTCCCGACCGGAGTCGGAGCCGCCATAGGTGGATACGCCGGCGACGCCTTGCCGGTGGCTCGCACTCTCGCCTCCGTCGTCGATTGTCTCATCACTCATCCCAATGTGCTGAATGCAGCAATGCTCTATTGGCCCATGCCTAATGCTTTGTATGTCGAAGGTTATGCTCTCGACCGATTCGCACAAGGCTTATGGGCTCTGAAGCCTGTTCACCAGAACAAGGTGGGGTTGGTTCTTGATTGTGGAATGGAAGAAGAGCTTCGCATTCGTCATTTGCAAGTGGCTGATGCTGCAAGGGCTTCCCTTGGCCTGCCTGTAGTGGAATATGTTGTCACTGACACTCCTTTGCAGGTAGAGAAGTGGATTGATACAAAAAGTGGGCAATCAACGGGGAGCATTAAGCACCCTGAATCACTACTGAGGGCTGTGCAGGCTTTAATCAAACGCTCTCAAGTAAATGCCGTTGCAGTTGTTGGACGTTTCCTGGATGATGATTTTGAAGACACTGATGATTATCGACAAGGGATGGGAATTGATCTATTGGCAGGAGTTGAGGCAGTTATAAGCCATCTAGTAGTGAAAGAATTCCAAATTCCTTGTGCACATGCTCCTGCTTTATTACCTGTACCTCTGAGCCCATCTTTATGCCCAAAATCAGCTGCTGAGGAGTTGGGATACACATTCTTGCCTTGTGTGCTTGCTGGCCTTAGTAATGCACCACAATACTTGGTCAAGAACTCTGAGTCCGAGCATGGTTGCATACTGGCAAATGATGTTGATAGTGTCATCCTTCCTATAGATGCTTGTGGAGGATCTGGTGCTCTTGCTTTTGCACAAAGCAAAGCCAATAAGCCCCTTATAATTGCTGTGGAGGAAAATGAAACAGTCCTTGATGACACACCTGATAAAGTTGGCATCGAAGCAATCAATGTCTCAAACTATTGGGAAGCGATTGGTGTTGTTGCAGCTCACAAGGCTGGAGTAAATCCGCAGTCACTACGAAGAAATAGAATTAGCAGTCTTCAACGCGCTTCTCTATTGCCTACTAATGGTTATGCCGTTCAAAGTGCCACAACAAATGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3326

Protein Analysis

411

Amino Acids

44.01

Weight (kDa)

5.87

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3326 PF11805 45 - 383 7.8e-160 Protein of unknown function (DUF3326)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 710, 1007
Acc36I ACCTGC 1 cut(s) 487
AccII CGCG 1 cut(s) 1176
AciI CCGC 2 cut(s) 168, 1136
AclWI GGATC 1 cut(s) 967
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 2 cut(s) 731, 738
AcuI CTGAAG 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 1 cut(s) 774
AfiI CCNNNNNNNGG 2 cut(s) 367, 1196
AgsI TTSAA 5 cut(s) 64, 121, 647, 1172, 1211
AjnI CCWGG 1 cut(s) 630
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AleI CACNNNNGTG 1 cut(s) 137
AloI GAACNNNNNNTCC 2 cut(s) 875, 907
AluBI AGCT 3 cut(s) 406, 806, 1115
AluI AGCT 3 cut(s) 406, 806, 1115
Alw21I GWGCWC 2 cut(s) 751, 970
Alw26I GTCTC 4 cut(s) 97, 120, 239, 1084
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 1 cut(s) 967
AlwNI CAGNNNCTG 1 cut(s) 809
AoxI GGCC 5 cut(s) 39, 43, 278, 454, 850
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 4 cut(s) 263, 437, 806, 1112
ApoI RAATTY 2 cut(s) 731, 738
Asp700I GAANNNNTTC 2 cut(s) 405, 731
AspLEI GCGC 1 cut(s) 1178
AspS9I GGNCC 3 cut(s) 7, 39, 279
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 1 cut(s) 350
AvaII GGWCC 1 cut(s) 7
BaeGI GKGCMC 1 cut(s) 751
BaeI ACNNNNGTAYC 2 cut(s) 40, 73
BanII GRGCYC 2 cut(s) 346, 785
BbsI GAAGAC 2 cut(s) 654, 1160
Bbv12I GWGCWC 2 cut(s) 751, 970
BbvCI CCTCAGC 1 cut(s) 810
BbvI GCAGC 4 cut(s) 275, 424, 793, 1124
BccI CCATC 3 cut(s) 667, 723, 793
BceAI ACGGC 3 cut(s) 3, 593, 1190
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 1 cut(s) 632
BciVI GTATCC 2 cut(s) 173, 815
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 4 cut(s) 97, 120, 239, 1084
BfaI CTAG 2 cut(s) 135, 719
BfmI CTRYAG 2 cut(s) 462, 942
BfuAI ACCTGC 1 cut(s) 487
BfuI GTATCC 2 cut(s) 173, 815
BisI GCNGC 5 cut(s) 168, 264, 438, 807, 1113
BlsI GCNGC 5 cut(s) 169, 265, 439, 808, 1114
Bme1390I CCNGG 2 cut(s) 38, 632
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 3 cut(s) 7, 39, 279
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 2 cut(s) 38, 632
BmsI GCATC 3 cut(s) 424, 937, 1071
BoxI GACNNNNGTC 1 cut(s) 158
BpiI GAAGAC 2 cut(s) 654, 1160
BpmI CTGGAG 1 cut(s) 1146
Bpu10I CCTNAGC 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 582
BpuMI CCSGG 1 cut(s) 38
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 472, 502
Bsc4I CCNNNNNNNGG 2 cut(s) 367, 1196
Bse118I RCCGGY 3 cut(s) 41, 185, 199
Bse1I ACTGG 1 cut(s) 915
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 1 cut(s) 632
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 4 cut(s) 244, 640, 678, 933
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 2 cut(s) 367, 1196
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 5 cut(s) 5, 557, 770, 801, 879
BseNI ACTGG 1 cut(s) 915
BseRI GAGGAG 1 cut(s) 827
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 4 cut(s) 275, 424, 793, 1124
BsgI GTGCAG 1 cut(s) 596
Bsh1236I CGCG 1 cut(s) 1176
Bsh1285I CGRYCG 2 cut(s) 156, 322
BshFI GGCC 5 cut(s) 41, 45, 280, 456, 852
BsiEI CGRYCG 2 cut(s) 156, 322
BsiHKAI GWGCWC 2 cut(s) 751, 970
BsiSI CCGG 5 cut(s) 37, 42, 156, 186, 200
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 2 cut(s) 367, 1196
BsmAI GTCTC 4 cut(s) 97, 120, 239, 1084
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 134
BsmI GAATGC 2 cut(s) 265, 411
BsnI GGCC 5 cut(s) 41, 45, 280, 456, 852
Bsp1286I GDGCHC 4 cut(s) 346, 751, 785, 970
Bsp143I GATC 2 cut(s) 682, 959
BspACI CCGC 2 cut(s) 168, 1136
BspANI GGCC 5 cut(s) 41, 45, 280, 456, 852
BspCNI CTCAG 5 cut(s) 6, 558, 771, 802, 880
BspFNI CGCG 1 cut(s) 1176
BspLI GGNNCC 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 1 cut(s) 967
BspQI GCTCTTC 1 cut(s) 396
BsrDI GCAATG 1 cut(s) 273
BsrFI RCCGGY 3 cut(s) 41, 185, 199
BsrI ACTGG 1 cut(s) 915
BssAI RCCGGY 3 cut(s) 41, 185, 199
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 2 cut(s) 682, 959
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 3 cut(s) 7, 75, 1033
Bst6I CTCTTC 1 cut(s) 396
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 7 cut(s) 43, 187, 208, 458, 579, 846, 850
BstDEI CTNAG 6 cut(s) 14, 566, 779, 810, 854, 888
BstF5I GGATG 4 cut(s) 244, 640, 678, 933
BstFNI CGCG 1 cut(s) 1176
BstHHI GCGC 1 cut(s) 1178
BstKTI GATC 2 cut(s) 685, 962
BstMAI GTCTC 4 cut(s) 97, 120, 239, 1084
BstMBI GATC 2 cut(s) 682, 959
BstMCI CGRYCG 2 cut(s) 156, 322
BstMWI GCNNNNNNNGC 8 cut(s) 96, 191, 277, 437, 443, 578, 841, 1121
BstNI CCWGG 1 cut(s) 632
BstNSI RCATGY 1 cut(s) 755
BstPAI GACNNNNGTC 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 36, 630
BstSFI CTRYAG 2 cut(s) 462, 942
BstSLI GKGCMC 1 cut(s) 751
BstUI CGCG 1 cut(s) 1176
BstV1I GCAGC 4 cut(s) 275, 424, 793, 1124
BstV2I GAAGAC 2 cut(s) 654, 1160
BstX2I RGATCY 1 cut(s) 959
BstYI RGATCY 1 cut(s) 959
BsuI GTATCC 2 cut(s) 173, 815
BsuRI GGCC 5 cut(s) 41, 45, 280, 456, 852
BtsCI GGATG 4 cut(s) 244, 640, 678, 933
BtsIMutI CAGTG 2 cut(s) 480, 651
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 7 cut(s) 43, 187, 208, 458, 579, 846, 850
CaiI CAGNNNCTG 1 cut(s) 809
CfoI GCGC 1 cut(s) 1178
Cfr10I RCCGGY 3 cut(s) 41, 185, 199
Cfr13I GGNCC 3 cut(s) 7, 39, 279
CseI GACGC 2 cut(s) 79, 200
Csp6I GTAC 1 cut(s) 773
CspCI CAANNNNNGTGG 2 cut(s) 855, 890
CviAII CATG 4 cut(s) 96, 283, 752, 899
CviQI GTAC 1 cut(s) 773
DdeI CTNAG 6 cut(s) 14, 566, 779, 810, 854, 888
DpnI GATC 2 cut(s) 684, 961
DpnII GATC 2 cut(s) 682, 959
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 2 cut(s) 346, 785
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 731
EcoRII CCWGG 1 cut(s) 630
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 2 cut(s) 346, 785
ErhI CCWWGG 1 cut(s) 451
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 4 cut(s) 99, 286, 755, 902
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 1 cut(s) 134
FatI CATG 4 cut(s) 95, 282, 751, 898
Fnu4HI GCNGC 5 cut(s) 168, 264, 438, 807, 1113
FokI GGATG 4 cut(s) 231, 647, 685, 920
FriOI GRGCYC 2 cut(s) 346, 785
FseI GGCCGGCC 1 cut(s) 45
Fsp4HI GCNGC 5 cut(s) 168, 264, 438, 807, 1113
FspBI CTAG 2 cut(s) 135, 719
GlaI GCGC 1 cut(s) 1177
GluI GCNGC 5 cut(s) 168, 264, 438, 807, 1113
GsuI CTGGAG 1 cut(s) 1146
HaeIII GGCC 5 cut(s) 41, 45, 280, 456, 852
HapII CCGG 5 cut(s) 37, 42, 156, 186, 200
HgaI GACGC 2 cut(s) 79, 200
HhaI GCGC 1 cut(s) 1178
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 4 cut(s) 99, 286, 755, 902
Hin6I GCGC 1 cut(s) 1176
HinP1I GCGC 1 cut(s) 1176
HinfI GANTC 4 cut(s) 159, 324, 557, 890
HpaII CCGG 5 cut(s) 37, 42, 156, 186, 200
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 2 cut(s) 358, 749
Hpy188I TCNGA 6 cut(s) 15, 164, 348, 780, 889, 895
Hpy188III TCNNGA 6 cut(s) 69, 121, 151, 317, 383, 880
Hpy8I GTNNAC 2 cut(s) 358, 749
Hpy99I CGWCG 3 cut(s) 194, 227, 230
HpyAV CCTTC 6 cut(s) 20, 39, 43, 127, 299, 947
HpyCH4III ACNGT 3 cut(s) 7, 75, 1033
HpyCH4IV ACGT 1 cut(s) 625
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 191, 277, 437, 443, 578, 841, 1121
HpyF3I CTNAG 6 cut(s) 14, 566, 779, 810, 854, 888
HpySE526I ACGT 1 cut(s) 625
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 4 cut(s) 99, 286, 755, 902
HspAI GCGC 1 cut(s) 1176
KroI GCCGGC 2 cut(s) 41, 185
KroNI GCCGGC 2 cut(s) 43, 187
Kzo9I GATC 2 cut(s) 682, 959
LguI GCTCTTC 1 cut(s) 396
LmnI GCTCC 3 cut(s) 164, 540, 760
Lsp1109I GCAGC 4 cut(s) 275, 424, 793, 1124
LweI GCATC 3 cut(s) 424, 937, 1071
MaeI CTAG 2 cut(s) 135, 719
MaeII ACGT 1 cut(s) 625
MaeIII GTNAC 2 cut(s) 478, 1140
MalI GATC 2 cut(s) 684, 961
MboI GATC 2 cut(s) 682, 959
MboII GAAGA 6 cut(s) 52, 410, 413, 659, 1160, 1161
MflI RGATCY 1 cut(s) 959
MhlI GDGCHC 4 cut(s) 346, 751, 785, 970
MluCI AATT 5 cut(s) 678, 731, 738, 1008, 1158
MlyI GAGTC 2 cut(s) 168, 899
MmeI TCCRAC 2 cut(s) 142, 601
MnlI CCTC 8 cut(s) 62, 229, 561, 694, 786, 805, 950, 1012
MreI CGCCGGCG 1 cut(s) 185
MroNI GCCGGC 2 cut(s) 41, 185
MroXI GAANNNNTTC 2 cut(s) 405, 731
MseI TTAA 3 cut(s) 81, 546, 584
MslI CAYNNNNRTG 3 cut(s) 137, 249, 790
MspA1I CMGCKG 1 cut(s) 806
MspI CCGG 5 cut(s) 37, 42, 156, 186, 200
MspR9I CCNGG 2 cut(s) 38, 632
Mva1269I GAATGC 2 cut(s) 265, 411
MvaI CCWGG 1 cut(s) 632
MvnI CGCG 1 cut(s) 1176
MwoI GCNNNNNNNGC 8 cut(s) 96, 191, 277, 437, 443, 578, 841, 1121
NaeI GCCGGC 2 cut(s) 43, 187
NciI CCSGG 1 cut(s) 38
NdeII GATC 2 cut(s) 682, 959
NgoMIV GCCGGC 2 cut(s) 41, 185
NlaIII CATG 4 cut(s) 99, 286, 755, 902
NlaIV GGNNCC 1 cut(s) 166
NmuCI GTSAC 2 cut(s) 478, 1140
NspI RCATGY 1 cut(s) 755
OliI CACNNNNGTG 1 cut(s) 137
PciSI GCTCTTC 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 2 cut(s) 265, 411
PdiI GCCGGC 2 cut(s) 43, 187
PdmI GAANNNNTTC 2 cut(s) 405, 731
PfeI GAWTC 2 cut(s) 324, 557
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 5 cut(s) 169, 265, 439, 808, 1114
PleI GAGTC 2 cut(s) 167, 898
PpsI GAGTC 2 cut(s) 167, 898
PshAI GACNNNNGTC 1 cut(s) 158
PsiI TTATAA 2 cut(s) 710, 1007
Psp6I CCWGG 1 cut(s) 630
PspGI CCWGG 1 cut(s) 630
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 3 cut(s) 7, 39, 279
PstNI CAGNNNCTG 1 cut(s) 809
PsuI RGATCY 1 cut(s) 959
PvuII CAGCTG 1 cut(s) 806
RigI GGCCGGCC 1 cut(s) 45
RsaI GTAC 1 cut(s) 774
RsaNI GTAC 1 cut(s) 773
RseI CAYNNNNRTG 3 cut(s) 137, 249, 790
SapI GCTCTTC 1 cut(s) 396
SaqAI TTAA 3 cut(s) 81, 546, 584
SatI GCNGC 5 cut(s) 168, 264, 438, 807, 1113
Sau3AI GATC 2 cut(s) 682, 959
Sau96I GGNCC 3 cut(s) 7, 39, 279
SchI GAGTC 2 cut(s) 168, 899
ScrFI CCNGG 2 cut(s) 38, 632
SduI GDGCHC 4 cut(s) 346, 751, 785, 970
SfaNI GCATC 3 cut(s) 424, 937, 1071
SfcI CTRYAG 2 cut(s) 462, 942
SgrAI CRCCGGYG 1 cut(s) 185
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 3 cut(s) 137, 249, 790
SmlI CTYRAG 1 cut(s) 597
SmoI CTYRAG 1 cut(s) 597
Sse9I AATT 5 cut(s) 678, 731, 738, 1008, 1158
SsiI CCGC 2 cut(s) 168, 1136
SspMI CTAG 2 cut(s) 135, 719
StyD4I CCNGG 2 cut(s) 36, 630
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 3 cut(s) 7, 75, 1033
TaiI ACGT 1 cut(s) 628
TaqI TCGA 5 cut(s) 228, 303, 318, 664, 1065
TaqII GACCGA 1 cut(s) 336
TasI AATT 5 cut(s) 678, 731, 738, 1008, 1158
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 2 cut(s) 324, 557
Tru1I TTAA 3 cut(s) 81, 546, 584
Tru9I TTAA 3 cut(s) 81, 546, 584
TscAI CASTG 2 cut(s) 487, 658
TseFI GTSAC 2 cut(s) 478, 1140
TseI GCWGC 4 cut(s) 263, 437, 806, 1112
Tsp45I GTSAC 2 cut(s) 478, 1140
TspDTI ATGAA 1 cut(s) 1041
TspGWI ACGGA 2 cut(s) 44, 211
TspRI CASTG 2 cut(s) 487, 658
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 2 cut(s) 731, 738
XceI RCATGY 1 cut(s) 755
XmnI GAANNNNTTC 2 cut(s) 405, 731
XspI CTAG 2 cut(s) 135, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.