MD16G1029600.v1.1

Protein of unknown function (DUF3326)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004231
Physical Location & Seq
Reverse (-)
2124145 .. 2127679
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1029600.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGACAGTAATTCTGAGCCGTCCGTTCCTCTCCTTGCCCGTCCAAAACCGACGCTTCAACAAATCCCGACCGTCCGCTTCACCAAGCATCTCATGCTTCAATTCCCGGCACTCCTTCAAGAGAGACTACACGAGCGTAATGATAGTCCCCACCGGGATTGGAGCCGCCATCGGCGGATACGCCGGCGACGCCCTGCCCGTCGCCCGCACTCTCTCCTCCGTCGTCGATTGCCTCATCGCTCACCCCAACGTGCTGAATGCGGCGATGCTCTACTGGCCAATGCCGAATGCATTGTACGTCGAAGGCTATGCCCTTGACCGCTTTGCTGAAGGCCTATGGGGCCTCATGCCTGTTCACCAAAACAAGGTGGGTTTGGTTCTTGATTGTGGCATCGAGGAGGAGCTTCGTGTTCGCCATTTGCAAGTGGCGGATGCTGCGAGGGCTTCCCTTGGCTTGCCTGTTGTTGAATATGTTGTCACTGACACTCCATTGCAGGTAGAGAAGTGGGTTGATCCAAAAACTGGGCAATCAACAGGGAGGATTAAGCACCCTGATTCACTATTGAGGGCTGTGCAGGCTTTAGTTAAACGCTCGCAAGTAAATGCTGTTGCAGTTGTTGGACGCTTCCCTGACGATGATGATGTTCAAGACACAGACGATTATCGACAAGGGATGGGAATTGATCTATTGGCAGGGGTTGAGGCAGTTATAAGCCATCTAGTGGTGAAGGAGTTCCAAATTCCTTGTGCACATGCTCCTGCTTTATCACCCCTTCCCATGACCCCATCTCTATGCCCAAAATCAGCTGCTGAGGAGTTGGGGTACACATTCTTGCCATGTGTGCTTGCTGGGCTTAGTAATGCACCACAGTACTTGGTAAAGAACTCTGAGTCGGAGAATGGTTGCATACTTGCTAGTGATGTTGATAGCGTCATCCTTCCTATAGATGCTTTTGGAGGACATGGCGCTCTTGCTTTTGCACGAAGCAAAAGAAACAAGCCACTTATAATTGCTGTGGAGGAAAATGTAACAGTCCTTGATGATACACCAGATAAATTTGGGATTGAAGCGGTCAATGTCTCAAACTATTGGGAAGCAATTGGTGTCATTGCAGCACACAAGGCTGGAGTAAATCCGCATTCTCTACGAAGAAATAGGATTAAGAATCTTCAACAAACTTCTCTGTCGCCTGCCAATGGTTATGCCATTAAAATGCCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3326

Protein Analysis

408

Amino Acids

44.0

Weight (kDa)

6.37

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3326 PF11805 44 - 383 5.1e-161 Protein of unknown function (DUF3326)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 710, 1007
Acc36I ACCTGC 1 cut(s) 484
AccB7I CCANNNNNTGG 2 cut(s) 521, 721
AciI CCGC 9 cut(s) 75, 165, 174, 205, 260, 319, 428, 1070, 1136
AclWI GGATC 1 cut(s) 506
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 2 cut(s) 738, 1055
AcuI CTGAAG 1 cut(s) 348
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 3 cut(s) 296, 824, 872
AfiI CCNNNNNNNGG 4 cut(s) 364, 521, 721, 1196
AgsI TTSAA 7 cut(s) 58, 100, 118, 467, 647, 1067, 1172
AluBI AGCT 2 cut(s) 403, 806
AluI AGCT 2 cut(s) 403, 806
Alw21I GWGCWC 1 cut(s) 751
Alw26I GTCTC 2 cut(s) 117, 1084
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 1 cut(s) 506
AlwNI CAGNNNCTG 1 cut(s) 809
AoxI GGCC 3 cut(s) 275, 331, 340
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 3 cut(s) 434, 806, 1112
ApoI RAATTY 2 cut(s) 738, 1055
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 1 cut(s) 968
AspS9I GGNCC 1 cut(s) 340
AsuC2I CCSGG 2 cut(s) 106, 154
AsuHPI GGTGA 5 cut(s) 72, 233, 347, 736, 759
BaeGI GKGCMC 1 cut(s) 751
BalI TGGCCA 1 cut(s) 277
BauI CACGAG 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 751
BbvCI CCTCAGC 1 cut(s) 810
BbvI GCAGC 3 cut(s) 421, 793, 1124
BccI CCATC 4 cut(s) 176, 667, 723, 793
BceAI ACGGC 1 cut(s) 3
BcgI CGANNNNNNTGC 2 cut(s) 290, 324
BciVI GTATCC 1 cut(s) 170
BcnI CCSGG 2 cut(s) 106, 154
BcoDI GTCTC 2 cut(s) 117, 1084
BfaI CTAG 2 cut(s) 719, 915
BfmI CTRYAG 1 cut(s) 942
BfoI RGCGCY 1 cut(s) 969
BfuAI ACCTGC 1 cut(s) 484
BfuI GTATCC 1 cut(s) 170
BglI GCCNNNNNGGC 1 cut(s) 339
BisI GCNGC 5 cut(s) 165, 261, 435, 807, 1113
BlsI GCNGC 5 cut(s) 166, 262, 436, 808, 1114
BmcAI AGTACT 1 cut(s) 872
Bme1390I CCNGG 2 cut(s) 106, 154
BmgT120I GGNCC 1 cut(s) 340
BmiI GGNNCC 2 cut(s) 163, 341
BmrFI CCNGG 2 cut(s) 106, 154
BmrI ACTGGG 1 cut(s) 531
BmsI GCATC 5 cut(s) 96, 255, 399, 421, 937
BmuI ACTGGG 1 cut(s) 531
BpmI CTGGAG 1 cut(s) 1146
Bpu10I CCTNAGC 1 cut(s) 810
BpuMI CCSGG 2 cut(s) 106, 154
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 448
BsaXI ACNNNNNCTCC 4 cut(s) 469, 499, 806, 836
Bsc4I CCNNNNNNNGG 4 cut(s) 364, 521, 721, 1196
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 2 cut(s) 278, 526
Bse3DI GCAATG 2 cut(s) 488, 1107
BseDI CCNNGG 1 cut(s) 448
BseGI GGATG 3 cut(s) 436, 678, 933
BseLI CCNNNNNNNGG 4 cut(s) 364, 521, 721, 1196
BseMI GCAATG 2 cut(s) 488, 1107
BseMII CTCAG 3 cut(s) 5, 801, 879
BseNI ACTGG 2 cut(s) 278, 526
BseRI GAGGAG 4 cut(s) 205, 410, 413, 827
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 3 cut(s) 421, 793, 1124
BseYI CCCAGC 1 cut(s) 848
BsgI GTGCAG 1 cut(s) 593
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 3 cut(s) 277, 333, 342
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 751
BsiSI CCGG 3 cut(s) 106, 153, 183
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 4 cut(s) 364, 521, 721, 1196
BsmAI GTCTC 2 cut(s) 117, 1084
BsmFI GGGAC 1 cut(s) 131
BsmI GAATGC 3 cut(s) 262, 292, 1138
BsnI GGCC 3 cut(s) 277, 333, 342
Bsp1286I GDGCHC 1 cut(s) 751
Bsp143I GATC 2 cut(s) 511, 682
BspACI CCGC 9 cut(s) 75, 165, 174, 205, 260, 319, 428, 1070, 1136
BspANI GGCC 3 cut(s) 277, 333, 342
BspCNI CTCAG 3 cut(s) 6, 802, 880
BspLI GGNNCC 2 cut(s) 163, 341
BspMI ACCTGC 1 cut(s) 484
BspPI GGATC 1 cut(s) 506
BsrDI GCAATG 2 cut(s) 488, 1107
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 2 cut(s) 278, 526
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 448
BssMI GATC 2 cut(s) 511, 682
BssNI GRCGYC 1 cut(s) 189
BssSI CACGAG 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 448
Bst2BI CACGAG 1 cut(s) 130
Bst4CI ACNGT 5 cut(s) 7, 72, 870, 1033, 1221
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 93
BstC8I GCNNGC 7 cut(s) 184, 205, 455, 576, 593, 846, 1191
BstDEI CTNAG 4 cut(s) 14, 810, 854, 888
BstF5I GGATG 3 cut(s) 436, 678, 933
BstH2I RGCGCY 1 cut(s) 969
BstHHI GCGC 1 cut(s) 968
BstKTI GATC 2 cut(s) 514, 685
BstMAI GTCTC 2 cut(s) 117, 1084
BstMBI GATC 2 cut(s) 511, 682
BstMCI CGRYCG 1 cut(s) 71
BstNSI RCATGY 1 cut(s) 755
BstSCI CCNGG 2 cut(s) 104, 152
BstSFI CTRYAG 1 cut(s) 942
BstSLI GKGCMC 1 cut(s) 751
BstV1I GCAGC 3 cut(s) 421, 793, 1124
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 3 cut(s) 277, 333, 342
BtgZI GCGATG 2 cut(s) 220, 278
BtsCI GGATG 3 cut(s) 436, 678, 933
BtsIMutI CAGTG 1 cut(s) 477
BveI ACCTGC 1 cut(s) 484
Cac8I GCNNGC 7 cut(s) 184, 205, 455, 576, 593, 846, 1191
CaiI CAGNNNCTG 1 cut(s) 809
CfoI GCGC 1 cut(s) 968
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 340
CseI GACGC 4 cut(s) 60, 197, 630, 919
Csp6I GTAC 3 cut(s) 295, 823, 871
CspCI CAANNNNNGTGG 4 cut(s) 139, 174, 855, 890
CviAII CATG 6 cut(s) 93, 346, 752, 778, 837, 962
CviQI GTAC 3 cut(s) 295, 823, 871
DdeI CTNAG 4 cut(s) 14, 810, 854, 888
DpnI GATC 2 cut(s) 513, 684
DpnII GATC 2 cut(s) 511, 682
EaeI YGGCCR 1 cut(s) 275
EciI GGCGGA 2 cut(s) 189, 443
Eco130I CCWWGG 1 cut(s) 448
Eco147I AGGCCT 1 cut(s) 333
Eco57I CTGAAG 1 cut(s) 348
EcoO109I RGGNCCY 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 448
EcoT22I ATGCAT 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 448
FaeI CATG 6 cut(s) 96, 349, 755, 781, 840, 965
FaqI GGGAC 1 cut(s) 131
FatI CATG 6 cut(s) 92, 345, 751, 777, 836, 961
FauI CCCGC 1 cut(s) 212
Fnu4HI GCNGC 5 cut(s) 165, 261, 435, 807, 1113
FokI GGATG 3 cut(s) 443, 685, 920
Fsp4HI GCNGC 5 cut(s) 165, 261, 435, 807, 1113
FspBI CTAG 2 cut(s) 719, 915
GlaI GCGC 1 cut(s) 967
GluI GCNGC 5 cut(s) 165, 261, 435, 807, 1113
GsaI CCCAGC 1 cut(s) 852
GsuI CTGGAG 1 cut(s) 1146
HaeII RGCGCY 1 cut(s) 969
HaeIII GGCC 3 cut(s) 277, 333, 342
HapII CCGG 3 cut(s) 106, 153, 183
HgaI GACGC 4 cut(s) 60, 197, 630, 919
HhaI GCGC 1 cut(s) 968
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 6 cut(s) 96, 349, 755, 781, 840, 965
Hin6I GCGC 1 cut(s) 966
HinP1I GCGC 1 cut(s) 966
HinfI GANTC 3 cut(s) 554, 890, 1165
HpaII CCGG 3 cut(s) 106, 153, 183
HphI GGTGA 5 cut(s) 72, 233, 347, 736, 759
Hpy166II GTNNAC 3 cut(s) 355, 749, 825
Hpy188I TCNGA 3 cut(s) 15, 889, 895
Hpy188III TCNNGA 4 cut(s) 66, 118, 380, 647
Hpy8I GTNNAC 3 cut(s) 355, 749, 825
Hpy99I CGWCG 6 cut(s) 54, 191, 203, 224, 227, 302
HpyAV CCTTC 6 cut(s) 124, 296, 323, 721, 782, 947
HpyCH4III ACNGT 5 cut(s) 7, 72, 870, 1033, 1221
HpyCH4IV ACGT 2 cut(s) 249, 297
HpyF3I CTNAG 4 cut(s) 14, 810, 854, 888
HpySE526I ACGT 2 cut(s) 249, 297
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 6 cut(s) 96, 349, 755, 781, 840, 965
HspAI GCGC 1 cut(s) 966
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Kzo9I GATC 2 cut(s) 511, 682
LmnI GCTCC 3 cut(s) 161, 400, 760
Lsp1109I GCAGC 3 cut(s) 421, 793, 1124
LweI GCATC 5 cut(s) 96, 255, 399, 421, 937
MaeI CTAG 2 cut(s) 719, 915
MaeII ACGT 2 cut(s) 249, 297
MaeIII GTNAC 2 cut(s) 475, 1027
MalI GATC 2 cut(s) 513, 684
MboI GATC 2 cut(s) 511, 682
MboII GAAGA 2 cut(s) 1160, 1161
MfeI CAATTG 1 cut(s) 1098
MhlI GDGCHC 1 cut(s) 751
MlsI TGGCCA 1 cut(s) 277
MluCI AATT 7 cut(s) 9, 100, 678, 738, 1008, 1055, 1098
MluNI TGGCCA 1 cut(s) 277
MlyI GAGTC 1 cut(s) 899
MmeI TCCRAC 2 cut(s) 598, 873
Mox20I TGGCCA 1 cut(s) 277
Mph1103I ATGCAT 1 cut(s) 292
MreI CGCCGGCG 1 cut(s) 182
MroNI GCCGGC 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 731
MscI TGGCCA 1 cut(s) 277
MseI TTAA 4 cut(s) 543, 585, 1161, 1209
MslI CAYNNNNRTG 2 cut(s) 790, 1211
Msp20I TGGCCA 1 cut(s) 277
MspA1I CMGCKG 1 cut(s) 806
MspI CCGG 3 cut(s) 106, 153, 183
MspR9I CCNGG 2 cut(s) 106, 154
MunI CAATTG 1 cut(s) 1098
Mva1269I GAATGC 3 cut(s) 262, 292, 1138
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 2 cut(s) 106, 154
NdeII GATC 2 cut(s) 511, 682
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 6 cut(s) 96, 349, 755, 781, 840, 965
NlaIV GGNNCC 2 cut(s) 163, 341
NmuCI GTSAC 1 cut(s) 475
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 1 cut(s) 755
PceI AGGCCT 1 cut(s) 333
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 3 cut(s) 262, 292, 1138
PdiI GCCGGC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 2 cut(s) 554, 1165
PflMI CCANNNNNTGG 2 cut(s) 521, 721
PkrI GCNGC 5 cut(s) 166, 262, 436, 808, 1114
PleI GAGTC 1 cut(s) 898
PpsI GAGTC 1 cut(s) 898
PsiI TTATAA 2 cut(s) 710, 1007
PspFI CCCAGC 1 cut(s) 848
PspN4I GGNNCC 2 cut(s) 163, 341
PspPI GGNCC 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 809
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 3 cut(s) 296, 824, 872
RsaNI GTAC 3 cut(s) 295, 823, 871
RseI CAYNNNNRTG 2 cut(s) 790, 1211
SaqAI TTAA 4 cut(s) 543, 585, 1161, 1209
SatI GCNGC 5 cut(s) 165, 261, 435, 807, 1113
Sau3AI GATC 2 cut(s) 511, 682
Sau96I GGNCC 1 cut(s) 340
ScaI AGTACT 1 cut(s) 872
SchI GAGTC 1 cut(s) 899
ScrFI CCNGG 2 cut(s) 106, 154
SduI GDGCHC 1 cut(s) 751
SetI ASST 6 cut(s) 252, 300, 369, 405, 498, 808
SfaNI GCATC 5 cut(s) 96, 255, 399, 421, 937
SfcI CTRYAG 1 cut(s) 942
SfiI GGCCNNNNNGGCC 1 cut(s) 339
SgrAI CRCCGGYG 1 cut(s) 182
SmiMI CAYNNNNRTG 2 cut(s) 790, 1211
Sse9I AATT 7 cut(s) 9, 100, 678, 738, 1008, 1055, 1098
SseBI AGGCCT 1 cut(s) 333
SsiI CCGC 9 cut(s) 75, 165, 174, 205, 260, 319, 428, 1070, 1136
SspMI CTAG 2 cut(s) 719, 915
StuI AGGCCT 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 104, 152
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 5 cut(s) 7, 72, 870, 1033, 1221
TaiI ACGT 2 cut(s) 252, 300
TaqI TCGA 4 cut(s) 225, 300, 393, 664
TasI AATT 7 cut(s) 9, 100, 678, 738, 1008, 1055, 1098
TatI WGTACW 1 cut(s) 870
TauI GCSGC 2 cut(s) 167, 263
TfiI GAWTC 2 cut(s) 554, 1165
Tru1I TTAA 4 cut(s) 543, 585, 1161, 1209
Tru9I TTAA 4 cut(s) 543, 585, 1161, 1209
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 3 cut(s) 434, 806, 1112
Tsp45I GTSAC 1 cut(s) 475
TspGWI ACGGA 2 cut(s) 12, 208
TspRI CASTG 1 cut(s) 484
Van91I CCANNNNNTGG 2 cut(s) 521, 721
VneI GTGCAC 1 cut(s) 747
XapI RAATTY 2 cut(s) 738, 1055
XceI RCATGY 1 cut(s) 755
XmnI GAANNNNTTC 1 cut(s) 731
XspI CTAG 2 cut(s) 719, 915
ZrmI AGTACT 1 cut(s) 872
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.