FvH4_4g35120

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013490
Physical Location & Seq
Reverse (-)
32540227 .. 32545807
5581 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35120.t1

Sequence Viewer

Length: 1236 bp
ATGCCGTCGAACAACAAATCGAAATCGGAGAAGCCCGAAGCTGCGAAACAGCTCCGGCGAGACCCCTATGAGGTCCTCGGCGTCTCCAGGAACTCCACCGACCAGGAAATCAAAACCGCTTACCGGAAAATGGCCCTCAAGTACCATCCTGACAAGAATGCAAATGACCCTAAAGCAGCTGATATTTTTAAGGAGGTCACATTTTCTTATAATATCTTGTCTGATCCAGACAAACGGCGGCAGTTTGACACATCTGGCTTTGAGGCTGTTGAATCAGAAAGCCAAGAATTGGAGTTGGATCTTTCAAGCTTGGGTGCTGTAAACACCATGTTTGCTGCTCTTTTTAGTAAACTCGGGGTGCCAATCAAGACTACTGTGTCAGCTACTGTCCTTGAGGAGGCACTAAATGGCATGGTCACTGTTCGTCCACTTTTATTGGGGCAGCCTATAACAAAAAAGGTTGAGAAGCAATGTTGTCACTTCTATTCAGTCGAAATATCTGAAAGCGAAGCACGAGCTGGATTGGTTTGCCAAGTGCATTCATCTGACAGAAGCAAGTTCAAGTTGTTATATTTTGATCGGGAAGACAATGGTGGATTGGGCCTTTGCCTACAGGAGGACAGTGCAAAAGCAGGAAAGGTTACATCTGCTGGGATGTATTTTCTTGGTTTCCCTGTGTATCGGTTGGATCACACATTGAACTCGATGGCTGCTGCAAAGGATCCAGATTCAGCCTTCTTCAAGAAGCTGGATGGGTTTCAGCCATGCGAATTAACCGAGCTGAAAGCTGGCACCCATGTGTTTGCTGTTTATGGTGACAACTTTTTTAAAAGTGCAACCTACACAATAGAAGTTCTTTCTTGTGAACCCTTTCTAGAAGAGAAGGAAAGTTTAAGGGCTGTGGAAGCTCAAATACTTACAAAACGTGTGGAGTTGTCAAAATTTGAGACAGAGTACAGAGAGGTTCTGGCACAATTCACAGAGATGACCAGTAGATATACACAAGAAATGCAAGAGATTGATGAGCTTCTCAAGCAGAGGAATGAAATACATGCGTTGTATACAACTGCTCCTCCAATAAAGCGGAGTTCAAGCAGGAGTAGAAATAGGGGTCATTCTAAGGAGGAAAAAGAAGATGCTCCAGTGAGAGAGAAGAAATCTACATTCAGAGATCGAACTAAGAAGAAGAAATGGTTCAACATTCACTTAAAGGTTGACAAAAGAAAACCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

412

Amino Acids

46.72

Weight (kDa)

8.46

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 21 - 83 8.7e-26 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013490)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24120
fragaria_vesca FvH4_4g35120
malus_domestica MD13G1086100.v1.1 MD16G1085500.v1.1
prunus_persica Prupe.1G260600_v2.0.a1 Prupe.1G260600_v2.0.a1
pyrus_communis pycom16g07390
rosa_chinensis RchiOBHm_Chr4g0444721
rosa_laevigata RLG00000005809
rosa_multiflora Rmu_sc0012443.1_g000009
rosa_roxburghii Rroxscaffold_5G00385230
rosa_rugosa Rorug04G0354700 Rorug04G0354800 Rorug04G0354900
rosa_samantha Rh4BG426600 Rh4CG441400 Rh4DG422400
rosa_wichuraiana Rw4G035650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 210
AasI GACNNNNNNGTC 1 cut(s) 376
AccB1I GGYRCC 2 cut(s) 358, 791
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 1 cut(s) 1061
AciI CCGC 3 cut(s) 117, 238, 1084
AclWI GGATC 5 cut(s) 218, 306, 696, 716, 729
AcsI RAATTY 1 cut(s) 941
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 2 cut(s) 143, 956
AfiI CCNNNNNNNGG 6 cut(s) 70, 123, 130, 289, 397, 616
AflIII ACRYGT 1 cut(s) 925
AgsI TTSAA 7 cut(s) 272, 306, 562, 700, 742, 1092, 1198
AjnI CCWGG 2 cut(s) 86, 102
AleI CACNNNNGTG 1 cut(s) 797
Alw26I GTCTC 3 cut(s) 54, 88, 941
AlwI GGATC 5 cut(s) 218, 306, 696, 716, 729
AlwNI CAGNNNCTG 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 2 cut(s) 132, 601
ApeKI GCWGC 6 cut(s) 41, 176, 335, 442, 710, 713
ApoI RAATTY 1 cut(s) 941
Asp700I GAANNNNTTC 2 cut(s) 870, 1193
AspS9I GGNCC 3 cut(s) 73, 133, 601
AsuHPI GGTGA 1 cut(s) 827
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 1 cut(s) 73
BamHI GGATCC 1 cut(s) 721
BanI GGYRCC 2 cut(s) 358, 791
BarI GAAGNNNNNNTAC 2 cut(s) 897, 929
BauI CACGAG 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 591
BbvI GCAGC 6 cut(s) 28, 188, 322, 454, 697, 700
BccI CCATC 3 cut(s) 153, 700, 746
BceAI ACGGC 1 cut(s) 251
BciT130I CCWGG 2 cut(s) 88, 104
BcoDI GTCTC 3 cut(s) 54, 88, 941
BfaI CTAG 1 cut(s) 875
BfmI CTRYAG 1 cut(s) 611
BisI GCNGC 7 cut(s) 42, 177, 239, 336, 443, 711, 714
BlsI GCNGC 7 cut(s) 43, 178, 240, 337, 444, 712, 715
Bme1390I CCNGG 2 cut(s) 88, 104
Bme18I GGWCC 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 3 cut(s) 73, 133, 601
BmiI GGNNCC 3 cut(s) 360, 723, 793
BmrFI CCNGG 2 cut(s) 88, 104
BmsI GCATC 1 cut(s) 1126
BpiI GAAGAC 1 cut(s) 591
BpmI CTGGAG 2 cut(s) 70, 1125
BpuEI CTTGAG 3 cut(s) 122, 413, 1016
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 76
BsaWI WCCGGW 1 cut(s) 123
BsaXI ACNNNNNCTCC 4 cut(s) 1054, 1057, 1084, 1087
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 123, 130, 289, 397, 616
Bse1I ACTGG 2 cut(s) 990, 1142
Bse3DI GCAATG 1 cut(s) 476
BseBI CCWGG 2 cut(s) 88, 104
BseDI CCNNGG 1 cut(s) 76
BseGI GGATG 3 cut(s) 145, 660, 757
BseLI CCNNNNNNNGG 6 cut(s) 70, 123, 130, 289, 397, 616
BseMI GCAATG 1 cut(s) 476
BseNI ACTGG 2 cut(s) 990, 1142
BseRI GAGGAG 2 cut(s) 410, 1062
BseXI GCAGC 6 cut(s) 28, 188, 322, 454, 697, 700
BseYI CCCAGC 1 cut(s) 650
BshFI GGCC 2 cut(s) 134, 603
BshNI GGYRCC 2 cut(s) 358, 791
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 2 cut(s) 55, 124
BslI CCNNNNNNNGG 6 cut(s) 70, 123, 130, 289, 397, 616
BsmAI GTCTC 3 cut(s) 54, 88, 941
BsmBI CGTCTC 1 cut(s) 88
BsmI GAATGC 2 cut(s) 163, 538
BsnI GGCC 2 cut(s) 134, 603
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 353
Bsp143I GATC 6 cut(s) 223, 298, 577, 688, 721, 1171
BspACI CCGC 3 cut(s) 117, 238, 1084
BspANI GGCC 2 cut(s) 134, 603
BspLI GGNNCC 3 cut(s) 360, 723, 793
BspPI GGATC 5 cut(s) 218, 306, 696, 716, 729
BspT107I GGYRCC 2 cut(s) 358, 791
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 476
BsrI ACTGG 2 cut(s) 990, 1142
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 6 cut(s) 223, 298, 577, 688, 721, 1171
BssNAI GTATAC 1 cut(s) 1062
BssNI GRCGYC 1 cut(s) 81
BssSI CACGAG 1 cut(s) 513
Bst1107I GTATAC 1 cut(s) 1062
Bst2BI CACGAG 1 cut(s) 513
Bst2UI CCWGG 2 cut(s) 88, 104
Bst4CI ACNGT 4 cut(s) 376, 388, 421, 623
Bst6I CTCTTC 1 cut(s) 873
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 790
BstDEI CTNAG 2 cut(s) 1119, 1179
BstENI CCTNNNNNAGG 2 cut(s) 395, 614
BstF5I GGATG 3 cut(s) 145, 660, 757
BstKTI GATC 6 cut(s) 226, 301, 580, 691, 724, 1174
BstMAI GTCTC 3 cut(s) 54, 88, 941
BstMBI GATC 6 cut(s) 223, 298, 577, 688, 721, 1171
BstMWI GCNNNNNNNGC 2 cut(s) 905, 1033
BstNI CCWGG 2 cut(s) 88, 104
BstNSI RCATGY 1 cut(s) 1055
BstSCI CCNGG 2 cut(s) 86, 102
BstSFI CTRYAG 1 cut(s) 611
BstV1I GCAGC 6 cut(s) 28, 188, 322, 454, 697, 700
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 2 cut(s) 298, 721
BstYI RGATCY 2 cut(s) 298, 721
BstZ17I GTATAC 1 cut(s) 1062
BsuRI GGCC 2 cut(s) 134, 603
BtsCI GGATG 3 cut(s) 145, 660, 757
BtsIMutI CAGTG 3 cut(s) 417, 628, 1149
Cac8I GCNNGC 1 cut(s) 790
CaiI CAGNNNCTG 1 cut(s) 386
Cfr13I GGNCC 3 cut(s) 73, 133, 601
CseI GACGC 1 cut(s) 70
Csp6I GTAC 2 cut(s) 142, 955
CspCI CAANNNNNGTGG 4 cut(s) 417, 452, 909, 944
CviAII CATG 5 cut(s) 328, 412, 765, 797, 1052
CviQI GTAC 2 cut(s) 142, 955
DdeI CTNAG 2 cut(s) 1119, 1179
DpnI GATC 6 cut(s) 225, 300, 579, 690, 723, 1173
DpnII GATC 6 cut(s) 223, 298, 577, 688, 721, 1171
DraI TTTAAA 1 cut(s) 829
DrdI GACNNNNNNGTC 1 cut(s) 376
DseDI GACNNNNNNGTC 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 873
EarI CTCTTC 1 cut(s) 873
Eco31I GGTCTC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 353
EcoNI CCTNNNNNAGG 2 cut(s) 395, 614
EcoO109I RGGNCCY 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 86, 102
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 5 cut(s) 331, 415, 768, 800, 1055
FalI AAGNNNNNCTT 1 cut(s) 1213
FatI CATG 5 cut(s) 327, 411, 764, 796, 1051
FblI GTMKAC 1 cut(s) 1061
Fnu4HI GCNGC 7 cut(s) 42, 177, 239, 336, 443, 711, 714
FokI GGATG 3 cut(s) 132, 667, 764
Fsp4HI GCNGC 7 cut(s) 42, 177, 239, 336, 443, 711, 714
FspBI CTAG 1 cut(s) 875
GluI GCNGC 7 cut(s) 42, 177, 239, 336, 443, 711, 714
GsaI CCCAGC 1 cut(s) 654
GsuI CTGGAG 2 cut(s) 70, 1125
HaeIII GGCC 2 cut(s) 134, 603
HapII CCGG 2 cut(s) 55, 124
HgaI GACGC 1 cut(s) 70
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 5 cut(s) 331, 415, 768, 800, 1055
HincII GTYRAC 1 cut(s) 1216
HindII GTYRAC 1 cut(s) 1216
HindIII AAGCTT 1 cut(s) 307
HinfI GANTC 2 cut(s) 272, 728
HpaII CCGG 2 cut(s) 55, 124
HphI GGTGA 1 cut(s) 827
Hpy166II GTNNAC 6 cut(s) 322, 350, 428, 866, 1062, 1216
Hpy188I TCNGA 6 cut(s) 28, 223, 277, 502, 547, 1169
Hpy188III TCNNGA 7 cut(s) 149, 227, 367, 581, 725, 742, 875
Hpy8I GTNNAC 6 cut(s) 322, 350, 428, 866, 1062, 1216
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 745, 877
HpyCH4III ACNGT 4 cut(s) 376, 388, 421, 623
HpyCH4IV ACGT 1 cut(s) 925
HpyCH4V TGCA 6 cut(s) 161, 538, 626, 716, 836, 1012
HpyF10VI GCNNNNNNNGC 2 cut(s) 905, 1033
HpyF3I CTNAG 2 cut(s) 1119, 1179
HpySE526I ACGT 1 cut(s) 925
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 5 cut(s) 331, 415, 768, 800, 1055
Kzo9I GATC 6 cut(s) 223, 298, 577, 688, 721, 1171
LmnI GCTCC 3 cut(s) 57, 1075, 1144
Lsp1109I GCAGC 6 cut(s) 28, 188, 322, 454, 697, 700
LweI GCATC 1 cut(s) 1126
MaeI CTAG 1 cut(s) 875
MaeII ACGT 1 cut(s) 925
MaeIII GTNAC 5 cut(s) 196, 415, 476, 640, 815
MalI GATC 6 cut(s) 225, 300, 579, 690, 723, 1173
MboI GATC 6 cut(s) 223, 298, 577, 688, 721, 1171
MboII GAAGA 7 cut(s) 596, 730, 890, 1145, 1165, 1195, 1198
MflI RGATCY 2 cut(s) 298, 721
MluCI AATT 4 cut(s) 287, 770, 941, 974
MmeI TCCRAC 2 cut(s) 276, 666
MroXI GAANNNNTTC 2 cut(s) 870, 1193
MseI TTAA 5 cut(s) 189, 773, 828, 893, 1208
MslI CAYNNNNRTG 2 cut(s) 797, 983
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 2 cut(s) 55, 124
MspR9I CCNGG 2 cut(s) 88, 104
Mva1269I GAATGC 2 cut(s) 163, 538
MvaI CCWGG 2 cut(s) 88, 104
MwoI GCNNNNNNNGC 2 cut(s) 905, 1033
NdeII GATC 6 cut(s) 223, 298, 577, 688, 721, 1171
NlaIII CATG 5 cut(s) 331, 415, 768, 800, 1055
NlaIV GGNNCC 3 cut(s) 360, 723, 793
NmeAIII GCCGAG 1 cut(s) 57
NmuCI GTSAC 4 cut(s) 196, 415, 476, 815
NspI RCATGY 1 cut(s) 1055
OliI CACNNNNGTG 1 cut(s) 797
PctI GAATGC 2 cut(s) 163, 538
PdmI GAANNNNTTC 2 cut(s) 870, 1193
PfeI GAWTC 2 cut(s) 272, 728
PflMI CCANNNNNTGG 1 cut(s) 289
PfoI TCCNGGA 1 cut(s) 86
PkrI GCNGC 7 cut(s) 43, 178, 240, 337, 444, 712, 715
PpuMI RGGWCCY 1 cut(s) 73
PsiI TTATAA 1 cut(s) 210
Psp5II RGGWCCY 1 cut(s) 73
Psp6I CCWGG 2 cut(s) 86, 102
PspFI CCCAGC 1 cut(s) 650
PspGI CCWGG 2 cut(s) 86, 102
PspN4I GGNNCC 3 cut(s) 360, 723, 793
PspPI GGNCC 3 cut(s) 73, 133, 601
PspPPI RGGWCCY 1 cut(s) 73
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 2 cut(s) 298, 721
PvuII CAGCTG 1 cut(s) 179
RsaI GTAC 2 cut(s) 143, 956
RsaNI GTAC 2 cut(s) 142, 955
RseI CAYNNNNRTG 2 cut(s) 797, 983
SaqAI TTAA 5 cut(s) 189, 773, 828, 893, 1208
SatI GCNGC 7 cut(s) 42, 177, 239, 336, 443, 711, 714
Sau3AI GATC 6 cut(s) 223, 298, 577, 688, 721, 1171
Sau96I GGNCC 3 cut(s) 73, 133, 601
ScrFI CCNGG 2 cut(s) 88, 104
SfaNI GCATC 1 cut(s) 1126
SfcI CTRYAG 1 cut(s) 611
SinI GGWCC 1 cut(s) 73
SmiMI CAYNNNNRTG 2 cut(s) 797, 983
SmlI CTYRAG 3 cut(s) 137, 392, 1031
SmoI CTYRAG 3 cut(s) 137, 392, 1031
Sse9I AATT 4 cut(s) 287, 770, 941, 974
SsiI CCGC 3 cut(s) 117, 238, 1084
SspMI CTAG 1 cut(s) 875
StyD4I CCNGG 2 cut(s) 86, 102
TaaI ACNGT 4 cut(s) 376, 388, 421, 623
TaiI ACGT 1 cut(s) 928
TaqI TCGA 5 cut(s) 8, 20, 492, 704, 1174
TasI AATT 4 cut(s) 287, 770, 941, 974
TatI WGTACW 1 cut(s) 954
TauI GCSGC 1 cut(s) 241
TfiI GAWTC 2 cut(s) 272, 728
Tru1I TTAA 5 cut(s) 189, 773, 828, 893, 1208
Tru9I TTAA 5 cut(s) 189, 773, 828, 893, 1208
TscAI CASTG 3 cut(s) 424, 628, 1149
TseFI GTSAC 4 cut(s) 196, 415, 476, 815
TseI GCWGC 6 cut(s) 41, 176, 335, 442, 710, 713
Tsp45I GTSAC 4 cut(s) 196, 415, 476, 815
TspDTI ATGAA 2 cut(s) 531, 1059
TspRI CASTG 3 cut(s) 424, 628, 1149
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 73
XagI CCTNNNNNAGG 2 cut(s) 395, 614
XapI RAATTY 1 cut(s) 941
XbaI TCTAGA 1 cut(s) 874
XceI RCATGY 1 cut(s) 1055
XmiI GTMKAC 1 cut(s) 1061
XmnI GAANNNNTTC 2 cut(s) 870, 1193
XspI CTAG 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.