MD13G1086100.v1.1

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013490
Physical Location & Seq
Forward (+)
6064283 .. 6068645
4363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1086100.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGCAGGGCAGCCGATCGAAATCGGAGAAGCAGGAAGCTGAGAAGCAGCTCCGGCGAGACCCTTATGAGGTCCTCGGCCTCAATAGGAATTCCACCGACCAGGAAATCAAAAGTGCTTACCGGAAAATGGCTCTCAAGTACCATCCTGACAAAAATGCTAATGACCCTAAAGCAGCTGATATCTTCAAGGAGGTCACGTTTTCTTATAATATATTGTCTGATCCAGACAAACGGCGTCAGTATGACACTGCCGGCTTTCAGGCTGTTGAAACAGAAAGCCACGACTTGGAGCTAGATCTTTCAAGTTTGGGTGCTGTAAATACTATTTTTGCTGCCCTTTTTAGTAAACTTGGAGTGCCAATAAAGACCACCGTATCAGCTACTGTCTTGGAGGAGGCACTAAATGGCAGGGTCACTGCTCGTCCACTTCCATTGGGGCACTCTATAACGAAAAAGGTTGAAAAGCAATGTGCTCACTTCTATTCTGTTACAATAACGGAAGCGGAAGCAAGAGCTGGATTTGTGTGCCAAGTGCAATCGTCGGACAAAAGCAAGTTCAAGCTATTATATTTTGATCAGGAAGAGACGGGTGGATTGAGCCTTTGCCTACAGGAGGATAGTGCAAAAACAGGGAAGGTTACATCTGCTGGGATGTATTTTCTTGGTTTCCCTGTATATCGATTGGATCAAACATCCAACTCGATGGCCAGTGCAAAGGATCCTGATGCTGCATTCTTCAAAAAGCTGGATGGGTTTCAGCCATGTGAATTAACCGAACTAAAAGCAGGCACCCATGTGTTTGCTGTTTATGGTGACAATTTTTTTAAAAGTGCAAGCTACACGATAGAAGTTCTTTCTTGTGGACCTTTTGCAGAAGAAAAGGAAAGTTTAAGGGCGGTGGAAGCTCAAATACTTTCAAAAAGAGTGGAGTTGTCCAAATTTGAAACTGAGTACAGAGAGGTTCTAGCACAATTTACAGAGATGACCTCCAGATACACACAAGAAATGCAAGAGATTGACGAGCTTCTCAGGCAGAGGAATGAAATTCATGCCTCTTATACTATTGCTCCTCCAATGAAGCGGAGTTCAAGTAGGAGTAGAAATAAGGGTCAGCCTAAGGAGGTCAAAGAAGATGGTCAGTTGAGAGATAAGAAACCTTCGTTTAGAGATCGATCCAAGAAGAGCAAATGGTTCAACCTTCACTTAAAGGGAATCAATCAGGCAATCTCTTTCCAGTTCTTGGTTCTGGTGGTTTTGGCATCCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

423

Amino Acids

47.6

Weight (kDa)

8.54

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 20 - 82 6.2e-26 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013490)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24120
fragaria_vesca FvH4_4g35120
malus_domestica MD13G1086100.v1.1 MD16G1085500.v1.1
prunus_persica Prupe.1G260600_v2.0.a1 Prupe.1G260600_v2.0.a1
pyrus_communis pycom16g07390
rosa_chinensis RchiOBHm_Chr4g0444721
rosa_laevigata RLG00000005809
rosa_multiflora Rmu_sc0012443.1_g000009
rosa_roxburghii Rroxscaffold_5G00385230
rosa_rugosa Rorug04G0354700 Rorug04G0354800 Rorug04G0354900
rosa_samantha Rh4BG426600 Rh4CG441400 Rh4DG422400
rosa_wichuraiana Rw4G035650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 788
AccB7I CCANNNNNTGG 2 cut(s) 286, 1240
AciI CCGC 3 cut(s) 503, 896, 1081
AclWI GGATC 5 cut(s) 215, 693, 713, 726, 1167
AcoI YGGCCR 1 cut(s) 705
AcsI RAATTY 3 cut(s) 88, 938, 1044
AcyI GRCGYC 1 cut(s) 235
AfaI GTAC 2 cut(s) 140, 953
AfiI CCNNNNNNNGG 5 cut(s) 67, 127, 286, 613, 1240
AjnI CCWGG 1 cut(s) 99
AleI CACNNNNGTG 1 cut(s) 794
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 2 cut(s) 51, 578
AlwI GGATC 5 cut(s) 215, 693, 713, 726, 1167
AlwNI CAGNNNCTG 1 cut(s) 383
AoxI GGCC 2 cut(s) 76, 705
ApeKI GCWGC 5 cut(s) 9, 46, 173, 332, 728
ApoI RAATTY 3 cut(s) 88, 938, 1044
AspS9I GGNCC 2 cut(s) 70, 863
AsuHPI GGTGA 1 cut(s) 824
AvaII GGWCC 2 cut(s) 70, 863
AxyI CCTNAGG 1 cut(s) 1116
BaeGI GKGCMC 1 cut(s) 441
BaeI ACNNNNGTAYC 2 cut(s) 357, 390
BalI TGGCCA 1 cut(s) 707
BamHI GGATCC 1 cut(s) 718
BanI GGYRCC 1 cut(s) 788
BarI GAAGNNNNNNTAC 2 cut(s) 894, 926
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 5 cut(s) 21, 58, 185, 319, 715
BccI CCATC 4 cut(s) 150, 697, 743, 1127
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 1 cut(s) 101
BclI TGATCA 1 cut(s) 574
BcoDI GTCTC 2 cut(s) 51, 578
BfaI CTAG 2 cut(s) 293, 965
BfmI CTRYAG 1 cut(s) 608
BglII AGATCT 1 cut(s) 295
BisI GCNGC 5 cut(s) 10, 47, 174, 333, 729
BlsI GCNGC 5 cut(s) 11, 48, 175, 334, 730
Bme1390I CCNGG 1 cut(s) 101
Bme18I GGWCC 2 cut(s) 70, 863
BmgT120I GGNCC 2 cut(s) 70, 863
BmiI GGNNCC 2 cut(s) 720, 790
BmrFI CCNGG 1 cut(s) 101
BmsI GCATC 1 cut(s) 715
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
BpmI CTGGAG 1 cut(s) 973
BpuEI CTTGAG 1 cut(s) 119
Bsa29I ATCGAT 2 cut(s) 679, 1171
BsaBI GATNNNNATC 1 cut(s) 19
BsaHI GRCGYC 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 73
BsaWI WCCGGW 1 cut(s) 120
BsaXI ACNNNNNCTCC 6 cut(s) 182, 212, 1051, 1075, 1081, 1105
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 127, 286, 613, 1240
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 2 cut(s) 708, 1234
Bse21I CCTNAGG 1 cut(s) 1116
Bse3DI GCAATG 1 cut(s) 473
Bse8I GATNNNNATC 1 cut(s) 19
BseBI CCWGG 1 cut(s) 101
BseCI ATCGAT 2 cut(s) 679, 1171
BseDI CCNNGG 1 cut(s) 73
BseGI GGATG 5 cut(s) 142, 657, 692, 754, 1259
BseJI GATNNNNATC 1 cut(s) 19
BseLI CCNNNNNNNGG 5 cut(s) 67, 127, 286, 613, 1240
BseMI GCAATG 1 cut(s) 473
BseMII CTCAG 3 cut(s) 30, 939, 1042
BseNI ACTGG 2 cut(s) 708, 1234
BseRI GAGGAG 2 cut(s) 407, 1059
BseSI GKGCMC 1 cut(s) 441
BseXI GCAGC 5 cut(s) 21, 58, 185, 319, 715
BseYI CCCAGC 1 cut(s) 647
Bsh1285I CGRYCG 1 cut(s) 17
BshFI GGCC 2 cut(s) 78, 707
BshNI GGYRCC 1 cut(s) 788
BshVI ATCGAT 2 cut(s) 679, 1171
BsiEI CGRYCG 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 475
BsiSI CCGG 3 cut(s) 52, 121, 252
BslI CCNNNNNNNGG 5 cut(s) 67, 127, 286, 613, 1240
BsmAI GTCTC 2 cut(s) 51, 578
BsmBI CGTCTC 1 cut(s) 578
BsmI GAATGC 1 cut(s) 731
BsnI GGCC 2 cut(s) 78, 707
Bso31I GGTCTC 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 441, 475
Bsp143I GATC 8 cut(s) 14, 220, 295, 574, 685, 718, 1168, 1172
BspACI CCGC 3 cut(s) 503, 896, 1081
BspANI GGCC 2 cut(s) 78, 707
BspCNI CTCAG 3 cut(s) 31, 940, 1041
BspDI ATCGAT 2 cut(s) 679, 1171
BspLI GGNNCC 2 cut(s) 720, 790
BspPI GGATC 5 cut(s) 215, 693, 713, 726, 1167
BspQI GCTCTTC 1 cut(s) 1175
BspT107I GGYRCC 1 cut(s) 788
BspTNI GGTCTC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 473
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 2 cut(s) 708, 1234
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 8 cut(s) 14, 220, 295, 574, 685, 718, 1168, 1172
BssNI GRCGYC 1 cut(s) 235
Bst2UI CCWGG 1 cut(s) 101
Bst4CI ACNGT 2 cut(s) 373, 385
Bst6I CTCTTC 2 cut(s) 576, 1175
BstACI GRCGYC 1 cut(s) 235
BstC8I GCNNGC 3 cut(s) 253, 787, 835
BstDEI CTNAG 4 cut(s) 39, 948, 1028, 1116
BstENI CCTNNNNNAGG 1 cut(s) 611
BstF5I GGATG 5 cut(s) 142, 657, 692, 754, 1259
BstKTI GATC 8 cut(s) 17, 223, 298, 577, 688, 721, 1171, 1175
BstMAI GTCTC 2 cut(s) 51, 578
BstMBI GATC 8 cut(s) 14, 220, 295, 574, 685, 718, 1168, 1172
BstMCI CGRYCG 1 cut(s) 17
BstMWI GCNNNNNNNGC 3 cut(s) 52, 902, 1030
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 99
BstSFI CTRYAG 1 cut(s) 608
BstSLI GKGCMC 1 cut(s) 441
BstV1I GCAGC 5 cut(s) 21, 58, 185, 319, 715
BstX2I RGATCY 2 cut(s) 295, 718
BstXI CCANNNNNNTGG 1 cut(s) 703
BstYI RGATCY 2 cut(s) 295, 718
Bsu15I ATCGAT 2 cut(s) 679, 1171
Bsu36I CCTNAGG 1 cut(s) 1116
BsuRI GGCC 2 cut(s) 78, 707
BsuTUI ATCGAT 2 cut(s) 679, 1171
BtsCI GGATG 5 cut(s) 142, 657, 692, 754, 1259
BtsI GCAGTG 2 cut(s) 246, 414
BtsIMutI CAGTG 3 cut(s) 246, 414, 715
Cac8I GCNNGC 3 cut(s) 253, 787, 835
CaiI CAGNNNCTG 1 cut(s) 383
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 2 cut(s) 70, 863
ClaI ATCGAT 2 cut(s) 679, 1171
CseI GACGC 1 cut(s) 224
Csp6I GTAC 2 cut(s) 139, 952
CspCI CAANNNNNGTGG 4 cut(s) 414, 449, 906, 941
CviAII CATG 3 cut(s) 762, 794, 1049
CviQI GTAC 2 cut(s) 139, 952
DdeI CTNAG 4 cut(s) 39, 948, 1028, 1116
DpnI GATC 8 cut(s) 16, 222, 297, 576, 687, 720, 1170, 1174
DpnII GATC 8 cut(s) 14, 220, 295, 574, 685, 718, 1168, 1172
DraI TTTAAA 1 cut(s) 826
EaeI YGGCCR 1 cut(s) 705
Eam1104I CTCTTC 2 cut(s) 576, 1175
EarI CTCTTC 2 cut(s) 576, 1175
Eco31I GGTCTC 1 cut(s) 51
Eco32I GATATC 1 cut(s) 181
Eco47I GGWCC 2 cut(s) 70, 863
Eco81I CCTNAGG 1 cut(s) 1116
EcoNI CCTNNNNNAGG 1 cut(s) 611
EcoO109I RGGNCCY 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 99
EcoRV GATATC 1 cut(s) 181
Esp3I CGTCTC 1 cut(s) 578
FaeI CATG 3 cut(s) 765, 797, 1052
FatI CATG 3 cut(s) 761, 793, 1048
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 5 cut(s) 10, 47, 174, 333, 729
FokI GGATG 5 cut(s) 129, 664, 679, 761, 1246
Fsp4HI GCNGC 5 cut(s) 10, 47, 174, 333, 729
FspBI CTAG 2 cut(s) 293, 965
GluI GCNGC 5 cut(s) 10, 47, 174, 333, 729
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 1 cut(s) 973
HaeIII GGCC 2 cut(s) 78, 707
HapII CCGG 3 cut(s) 52, 121, 252
HgaI GACGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 3 cut(s) 765, 797, 1052
HinfI GANTC 1 cut(s) 1212
HpaII CCGG 3 cut(s) 52, 121, 252
HphI GGTGA 1 cut(s) 824
Hpy166II GTNNAC 3 cut(s) 347, 425, 863
Hpy188I TCNGA 3 cut(s) 25, 220, 544
Hpy188III TCNNGA 5 cut(s) 146, 224, 578, 722, 990
Hpy8I GTNNAC 3 cut(s) 347, 425, 863
Hpy99I CGWCG 1 cut(s) 544
HpyAV CCTTC 3 cut(s) 628, 1167, 1208
HpyCH4III ACNGT 2 cut(s) 373, 385
HpyCH4IV ACGT 1 cut(s) 197
HpyCH4V TGCA 8 cut(s) 4, 535, 623, 713, 731, 833, 872, 1009
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 902, 1030
HpyF3I CTNAG 4 cut(s) 39, 948, 1028, 1116
HpySE526I ACGT 1 cut(s) 197
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 3 cut(s) 765, 797, 1052
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 8 cut(s) 14, 220, 295, 574, 685, 718, 1168, 1172
LguI GCTCTTC 1 cut(s) 1175
LmnI GCTCC 3 cut(s) 54, 289, 1072
Lsp1109I GCAGC 5 cut(s) 21, 58, 185, 319, 715
LweI GCATC 1 cut(s) 715
MaeI CTAG 2 cut(s) 293, 965
MaeII ACGT 1 cut(s) 197
MaeIII GTNAC 5 cut(s) 193, 412, 487, 637, 812
MalI GATC 8 cut(s) 16, 222, 297, 576, 687, 720, 1170, 1174
MboI GATC 8 cut(s) 14, 220, 295, 574, 685, 718, 1168, 1172
MboII GAAGA 6 cut(s) 175, 593, 727, 887, 1142, 1192
MflI RGATCY 2 cut(s) 295, 718
MhlI GDGCHC 2 cut(s) 441, 475
MlsI TGGCCA 1 cut(s) 707
MluCI AATT 6 cut(s) 88, 767, 817, 938, 971, 1044
MluNI TGGCCA 1 cut(s) 707
MmeI TCCRAC 2 cut(s) 522, 720
Mox20I TGGCCA 1 cut(s) 707
MroNI GCCGGC 1 cut(s) 251
MscI TGGCCA 1 cut(s) 707
MseI TTAA 5 cut(s) 770, 825, 890, 1205, 1267
MslI CAYNNNNRTG 1 cut(s) 794
Msp20I TGGCCA 1 cut(s) 707
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 3 cut(s) 52, 121, 252
MspR9I CCNGG 1 cut(s) 101
Mva1269I GAATGC 1 cut(s) 731
MvaI CCWGG 1 cut(s) 101
MwoI GCNNNNNNNGC 3 cut(s) 52, 902, 1030
NaeI GCCGGC 1 cut(s) 253
NdeII GATC 8 cut(s) 14, 220, 295, 574, 685, 718, 1168, 1172
NgoMIV GCCGGC 1 cut(s) 251
NlaIII CATG 3 cut(s) 765, 797, 1052
NlaIV GGNNCC 2 cut(s) 720, 790
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 3 cut(s) 193, 412, 812
OliI CACNNNNGTG 1 cut(s) 794
PciSI GCTCTTC 1 cut(s) 1175
PctI GAATGC 1 cut(s) 731
PdiI GCCGGC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 1212
PflMI CCANNNNNTGG 2 cut(s) 286, 1240
PkrI GCNGC 5 cut(s) 11, 48, 175, 334, 730
Ple19I CGATCG 1 cut(s) 17
PpuMI RGGWCCY 1 cut(s) 70
PsiI TTATAA 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 99
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 2 cut(s) 720, 790
PspPI GGNCC 2 cut(s) 70, 863
PspPPI RGGWCCY 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 2 cut(s) 295, 718
PvuI CGATCG 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 2 cut(s) 140, 953
RsaNI GTAC 2 cut(s) 139, 952
RseI CAYNNNNRTG 1 cut(s) 794
SapI GCTCTTC 1 cut(s) 1175
SaqAI TTAA 5 cut(s) 770, 825, 890, 1205, 1267
SatI GCNGC 5 cut(s) 10, 47, 174, 333, 729
Sau3AI GATC 8 cut(s) 14, 220, 295, 574, 685, 718, 1168, 1172
Sau96I GGNCC 2 cut(s) 70, 863
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 2 cut(s) 441, 475
SfaNI GCATC 1 cut(s) 715
SfcI CTRYAG 1 cut(s) 608
SinI GGWCC 2 cut(s) 70, 863
SmiMI CAYNNNNRTG 1 cut(s) 794
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 6 cut(s) 88, 767, 817, 938, 971, 1044
SsiI CCGC 3 cut(s) 503, 896, 1081
SspMI CTAG 2 cut(s) 293, 965
StyD4I CCNGG 1 cut(s) 99
TaaI ACNGT 2 cut(s) 373, 385
TaiI ACGT 1 cut(s) 200
TaqI TCGA 4 cut(s) 17, 679, 701, 1171
TasI AATT 6 cut(s) 88, 767, 817, 938, 971, 1044
TatI WGTACW 1 cut(s) 951
TfiI GAWTC 1 cut(s) 1212
Tru1I TTAA 5 cut(s) 770, 825, 890, 1205, 1267
Tru9I TTAA 5 cut(s) 770, 825, 890, 1205, 1267
TscAI CASTG 3 cut(s) 253, 421, 715
TseFI GTSAC 3 cut(s) 193, 412, 812
TseI GCWGC 5 cut(s) 9, 46, 173, 332, 728
Tsp45I GTSAC 3 cut(s) 193, 412, 812
TspDTI ATGAA 3 cut(s) 1037, 1056, 1091
TspGWI ACGGA 1 cut(s) 512
TspRI CASTG 3 cut(s) 253, 421, 715
Van91I CCANNNNNTGG 2 cut(s) 286, 1240
VpaK11BI GGWCC 2 cut(s) 70, 863
XagI CCTNNNNNAGG 1 cut(s) 611
XapI RAATTY 3 cut(s) 88, 938, 1044
XspI CTAG 2 cut(s) 293, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.