Rroxscaffold_5G00385230

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013490
Physical Location & Seq
Reverse (-)
64324381 .. 64329456
5076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00385230.1

Sequence Viewer

Length: 1233 bp
ATGCCGTCGAACCGATCGAAATCGGAGAAGCCTGAAGCTGCGAAGCAGCTCCGGCGAGACCCCTATGAGGTCCTCGGTGTCTCGAGGAACTCCACTGACCAGGAAATCAAAAGCGCTTACCGGAAAATGGCCCTCAAGTACCATCCTGACAAGAATGCAAATGACCCTAAAGCAGCTGATATTTTTAAGGAGGTCACATTTTCTTATAATATCTTGTCTGATCCAGACAAACGGCGGCAGTATGACACATCTGGCTTTGAGGCTGTTGAATCAGAAAGCCAAGAATTGGAGCTGGATCTTTCAAGTCTGGGTGCTGTAAATACCATGTTTGCTGCCCTTTTTAGTAAACTCGGGGTGCCAATTAAGACAACTGTTTCAGCTACTGTCCTGGAGGAGGCACTAAATGGCATGGTCACTGTTCGTCCACTTTTATTGGGGCAGCCGATAACAAAAAAGGTTGAGAAGCAATGTTGTCACTTCTATTCAGTCGAAATATCAGAAAGCGAAGCACGAGCTGGATTGGTTTGCCAAGTGCATTCATCTGACAAAAGCAAGTTCAAGTTGTTATATTTTGATCGGGAAGACAATGGTGGATTGGGCCTTTGCCTACAGGAGGACAGTGCAAAAACAGGAAAGGTTACATCTGCTGGGATGTATTTTCTTGGTTTCCCCGTATATCGGTTGGATCACACATTGAACTCGATGGCTGCTGCAAAGGATCCAGATTCAGCCTTCTTCAAAAAGCTGGATGGGTTTCAGCCATGTGAATTAACTGAGCTGAAAGCTGGCACCCATGTGTTTGCTGTTTATGGTGACAACTTTTTTAAAAGTGCAACCTACACAATAGAAGTTCTTTCTTGTGAACCCTTTTCAGAAGAGAAGGAAAGTTTAAGGGCTGTTGAAGCTCAAATACTTACAAAACGAGTGGAGTTGTCAAAATTTGAGACAGAGTACAGAGAGGTTCTGGCACAATTCACAGAGATGACCAGTAGATATACACAAGAAATGCAAGAGATTGATGAGCTTCTCAAGCAGAGGAATGAAATACATGCCTCATATACAAATGCTCCTCCAATAAAGCGGAGTTCAAGTAGGAGTAGAAATAGGGGTCATTCTAAGGAGGACAAAGAAGATGCTCCAGTGAGAGAGAAGAAATCTTCATTCAGAGATCGAACTAAGAAAAAGAAATGGTTCAACATTCACTTAAAGGTTGACAAAAGAAAACCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

410

Amino Acids

46.57

Weight (kDa)

8.45

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 20 - 82 1.3e-26 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013490)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24120
fragaria_vesca FvH4_4g35120
malus_domestica MD13G1086100.v1.1 MD16G1085500.v1.1
prunus_persica Prupe.1G260600_v2.0.a1 Prupe.1G260600_v2.0.a1
pyrus_communis pycom16g07390
rosa_chinensis RchiOBHm_Chr4g0444721
rosa_laevigata RLG00000005809
rosa_multiflora Rmu_sc0012443.1_g000009
rosa_roxburghii Rroxscaffold_5G00385230
rosa_rugosa Rorug04G0354700 Rorug04G0354800 Rorug04G0354900
rosa_samantha Rh4BG426600 Rh4CG441400 Rh4DG422400
rosa_wichuraiana Rw4G035650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
AccB1I GGYRCC 2 cut(s) 355, 788
AccB7I CCANNNNNTGG 1 cut(s) 286
AciI CCGC 2 cut(s) 235, 1081
AclWI GGATC 5 cut(s) 215, 303, 693, 713, 726
AcsI RAATTY 1 cut(s) 938
AcuI CTGAAG 1 cut(s) 54
AfaI GTAC 2 cut(s) 140, 953
AfeI AGCGCT 1 cut(s) 115
AfiI CCNNNNNNNGG 6 cut(s) 67, 127, 286, 394, 613, 678
AgsI TTSAA 8 cut(s) 269, 303, 559, 697, 739, 902, 1089, 1195
AjnI CCWGG 2 cut(s) 99, 387
AleI CACNNNNGTG 1 cut(s) 794
Alw26I GTCTC 3 cut(s) 51, 85, 938
AlwI GGATC 5 cut(s) 215, 303, 693, 713, 726
AlwNI CAGNNNCTG 1 cut(s) 383
Ama87I CYCGRG 2 cut(s) 82, 350
Aor51HI AGCGCT 1 cut(s) 115
AoxI GGCC 2 cut(s) 129, 598
ApeKI GCWGC 7 cut(s) 38, 46, 173, 332, 439, 707, 710
ApoI RAATTY 1 cut(s) 938
Asp700I GAANNNNTTC 1 cut(s) 1190
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 3 cut(s) 70, 130, 598
AsuHPI GGTGA 1 cut(s) 824
AvaI CYCGRG 2 cut(s) 82, 350
AvaII GGWCC 1 cut(s) 70
BamHI GGATCC 1 cut(s) 718
BanI GGYRCC 2 cut(s) 355, 788
BarI GAAGNNNNNNTAC 2 cut(s) 894, 926
BauI CACGAG 1 cut(s) 510
BbsI GAAGAC 1 cut(s) 588
BbvI GCAGC 7 cut(s) 25, 58, 185, 319, 451, 694, 697
BccI CCATC 3 cut(s) 150, 697, 743
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 2 cut(s) 101, 389
BcoDI GTCTC 3 cut(s) 51, 85, 938
BfmI CTRYAG 1 cut(s) 608
BfoI RGCGCY 1 cut(s) 117
BisI GCNGC 8 cut(s) 39, 47, 174, 236, 333, 440, 708, 711
BlsI GCNGC 8 cut(s) 40, 48, 175, 237, 334, 441, 709, 712
Bme1390I CCNGG 2 cut(s) 101, 389
Bme18I GGWCC 1 cut(s) 70
BmeT110I CYCGRG 2 cut(s) 82, 350
BmgT120I GGNCC 3 cut(s) 70, 130, 598
BmiI GGNNCC 3 cut(s) 357, 720, 790
BmrFI CCNGG 2 cut(s) 101, 389
BmsI GCATC 1 cut(s) 1123
BpiI GAAGAC 1 cut(s) 588
BpmI CTGGAG 2 cut(s) 410, 1122
BpuEI CTTGAG 2 cut(s) 119, 1013
BsaBI GATNNNNATC 1 cut(s) 19
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 73
BsaWI WCCGGW 1 cut(s) 120
BsaXI ACNNNNNCTCC 4 cut(s) 1051, 1075, 1081, 1105
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 127, 286, 394, 613, 678
Bse1I ACTGG 2 cut(s) 987, 1139
Bse3DI GCAATG 1 cut(s) 473
Bse8I GATNNNNATC 1 cut(s) 19
BseBI CCWGG 2 cut(s) 101, 389
BseDI CCNNGG 1 cut(s) 73
BseGI GGATG 3 cut(s) 142, 657, 754
BseJI GATNNNNATC 1 cut(s) 19
BseLI CCNNNNNNNGG 6 cut(s) 67, 127, 286, 394, 613, 678
BseMI GCAATG 1 cut(s) 473
BseMII CTCAG 1 cut(s) 765
BseNI ACTGG 2 cut(s) 987, 1139
BseRI GAGGAG 2 cut(s) 407, 1059
BseXI GCAGC 7 cut(s) 25, 58, 185, 319, 451, 694, 697
BseYI CCCAGC 1 cut(s) 647
Bsh1285I CGRYCG 1 cut(s) 17
BshFI GGCC 2 cut(s) 131, 600
BshNI GGYRCC 2 cut(s) 355, 788
BsiEI CGRYCG 1 cut(s) 17
BsiHKCI CYCGRG 2 cut(s) 82, 350
BsiSI CCGG 2 cut(s) 52, 121
BslI CCNNNNNNNGG 6 cut(s) 67, 127, 286, 394, 613, 678
BsmAI GTCTC 3 cut(s) 51, 85, 938
BsmI GAATGC 2 cut(s) 160, 535
BsnI GGCC 2 cut(s) 131, 600
Bso31I GGTCTC 1 cut(s) 51
BsoBI CYCGRG 2 cut(s) 82, 350
Bsp143I GATC 7 cut(s) 14, 220, 295, 574, 685, 718, 1168
BspACI CCGC 2 cut(s) 235, 1081
BspANI GGCC 2 cut(s) 131, 600
BspCNI CTCAG 1 cut(s) 766
BspLI GGNNCC 3 cut(s) 357, 720, 790
BspPI GGATC 5 cut(s) 215, 303, 693, 713, 726
BspT107I GGYRCC 2 cut(s) 355, 788
BspTNI GGTCTC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 473
BsrI ACTGG 2 cut(s) 987, 1139
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 7 cut(s) 14, 220, 295, 574, 685, 718, 1168
BssSI CACGAG 1 cut(s) 510
Bst2BI CACGAG 1 cut(s) 510
Bst2UI CCWGG 2 cut(s) 101, 389
Bst4CI ACNGT 4 cut(s) 373, 385, 418, 620
Bst6I CTCTTC 1 cut(s) 870
BstC8I GCNNGC 1 cut(s) 787
BstDEI CTNAG 3 cut(s) 774, 1116, 1176
BstENI CCTNNNNNAGG 2 cut(s) 392, 611
BstF5I GGATG 3 cut(s) 142, 657, 754
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 7 cut(s) 17, 223, 298, 577, 688, 721, 1171
BstMAI GTCTC 3 cut(s) 51, 85, 938
BstMBI GATC 7 cut(s) 14, 220, 295, 574, 685, 718, 1168
BstMCI CGRYCG 1 cut(s) 17
BstMWI GCNNNNNNNGC 3 cut(s) 52, 902, 1030
BstNI CCWGG 2 cut(s) 101, 389
BstNSI RCATGY 1 cut(s) 1052
BstSCI CCNGG 2 cut(s) 99, 387
BstSFI CTRYAG 1 cut(s) 608
BstV1I GCAGC 7 cut(s) 25, 58, 185, 319, 451, 694, 697
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 2 cut(s) 295, 718
BstYI RGATCY 2 cut(s) 295, 718
BsuRI GGCC 2 cut(s) 131, 600
BtsCI GGATG 3 cut(s) 142, 657, 754
BtsIMutI CAGTG 4 cut(s) 93, 414, 625, 1146
Cac8I GCNNGC 1 cut(s) 787
CaiI CAGNNNCTG 1 cut(s) 383
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 3 cut(s) 70, 130, 598
Csp6I GTAC 2 cut(s) 139, 952
CspCI CAANNNNNGTGG 4 cut(s) 414, 449, 906, 941
CviAII CATG 5 cut(s) 325, 409, 762, 794, 1049
CviQI GTAC 2 cut(s) 139, 952
DdeI CTNAG 3 cut(s) 774, 1116, 1176
DpnI GATC 7 cut(s) 16, 222, 297, 576, 687, 720, 1170
DpnII GATC 7 cut(s) 14, 220, 295, 574, 685, 718, 1168
DraI TTTAAA 1 cut(s) 826
Eam1104I CTCTTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 870
Eco31I GGTCTC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 70
Eco47III AGCGCT 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 54
Eco88I CYCGRG 2 cut(s) 82, 350
EcoNI CCTNNNNNAGG 2 cut(s) 392, 611
EcoO109I RGGNCCY 1 cut(s) 70
EcoRII CCWGG 2 cut(s) 99, 387
FaeI CATG 5 cut(s) 328, 412, 765, 797, 1052
FalI AAGNNNNNCTT 1 cut(s) 1210
FatI CATG 5 cut(s) 324, 408, 761, 793, 1048
Fnu4HI GCNGC 8 cut(s) 39, 47, 174, 236, 333, 440, 708, 711
FokI GGATG 3 cut(s) 129, 664, 761
Fsp4HI GCNGC 8 cut(s) 39, 47, 174, 236, 333, 440, 708, 711
GlaI GCGC 1 cut(s) 115
GluI GCNGC 8 cut(s) 39, 47, 174, 236, 333, 440, 708, 711
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 2 cut(s) 410, 1122
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 2 cut(s) 131, 600
HapII CCGG 2 cut(s) 52, 121
HhaI GCGC 1 cut(s) 116
Hin1II CATG 5 cut(s) 328, 412, 765, 797, 1052
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 1 cut(s) 1213
HindII GTYRAC 1 cut(s) 1213
HinfI GANTC 2 cut(s) 269, 725
HpaII CCGG 2 cut(s) 52, 121
HphI GGTGA 1 cut(s) 824
Hpy166II GTNNAC 4 cut(s) 347, 425, 863, 1213
Hpy188I TCNGA 7 cut(s) 25, 220, 274, 499, 544, 874, 1166
Hpy188III TCNNGA 5 cut(s) 82, 146, 224, 578, 722
Hpy8I GTNNAC 4 cut(s) 347, 425, 863, 1213
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 742, 874
HpyCH4III ACNGT 4 cut(s) 373, 385, 418, 620
HpyCH4V TGCA 6 cut(s) 158, 535, 623, 713, 833, 1009
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 902, 1030
HpyF3I CTNAG 3 cut(s) 774, 1116, 1176
Hsp92II CATG 5 cut(s) 328, 412, 765, 797, 1052
HspAI GCGC 1 cut(s) 114
Kzo9I GATC 7 cut(s) 14, 220, 295, 574, 685, 718, 1168
LmnI GCTCC 4 cut(s) 54, 289, 1072, 1141
Lsp1109I GCAGC 7 cut(s) 25, 58, 185, 319, 451, 694, 697
LweI GCATC 1 cut(s) 1123
MaeIII GTNAC 5 cut(s) 193, 412, 473, 637, 812
MalI GATC 7 cut(s) 16, 222, 297, 576, 687, 720, 1170
MboI GATC 7 cut(s) 14, 220, 295, 574, 685, 718, 1168
MboII GAAGA 6 cut(s) 593, 727, 887, 1142, 1149, 1162
MflI RGATCY 2 cut(s) 295, 718
MluCI AATT 5 cut(s) 284, 360, 767, 938, 971
MmeI TCCRAC 1 cut(s) 663
MroXI GAANNNNTTC 1 cut(s) 1190
MseI TTAA 6 cut(s) 186, 363, 770, 825, 890, 1205
MslI CAYNNNNRTG 2 cut(s) 794, 980
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 2 cut(s) 52, 121
MspR9I CCNGG 2 cut(s) 101, 389
Mva1269I GAATGC 2 cut(s) 160, 535
MvaI CCWGG 2 cut(s) 101, 389
MwoI GCNNNNNNNGC 3 cut(s) 52, 902, 1030
NdeII GATC 7 cut(s) 14, 220, 295, 574, 685, 718, 1168
NlaIII CATG 5 cut(s) 328, 412, 765, 797, 1052
NlaIV GGNNCC 3 cut(s) 357, 720, 790
NmuCI GTSAC 4 cut(s) 193, 412, 473, 812
NspI RCATGY 1 cut(s) 1052
OliI CACNNNNGTG 1 cut(s) 794
PaeR7I CTCGAG 1 cut(s) 82
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 2 cut(s) 160, 535
PdmI GAANNNNTTC 1 cut(s) 1190
PfeI GAWTC 2 cut(s) 269, 725
PflMI CCANNNNNTGG 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 8 cut(s) 40, 48, 175, 237, 334, 441, 709, 712
Ple19I CGATCG 1 cut(s) 17
PpuMI RGGWCCY 1 cut(s) 70
PsiI TTATAA 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 70
Psp6I CCWGG 2 cut(s) 99, 387
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 2 cut(s) 99, 387
PspN4I GGNNCC 3 cut(s) 357, 720, 790
PspPI GGNCC 3 cut(s) 70, 130, 598
PspPPI RGGWCCY 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 2 cut(s) 295, 718
PvuI CGATCG 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 2 cut(s) 140, 953
RsaNI GTAC 2 cut(s) 139, 952
RseI CAYNNNNRTG 2 cut(s) 794, 980
SaqAI TTAA 6 cut(s) 186, 363, 770, 825, 890, 1205
SatI GCNGC 8 cut(s) 39, 47, 174, 236, 333, 440, 708, 711
Sau3AI GATC 7 cut(s) 14, 220, 295, 574, 685, 718, 1168
Sau96I GGNCC 3 cut(s) 70, 130, 598
ScrFI CCNGG 2 cut(s) 101, 389
SfaNI GCATC 1 cut(s) 1123
SfcI CTRYAG 1 cut(s) 608
Sfr274I CTCGAG 1 cut(s) 82
SinI GGWCC 1 cut(s) 70
SlaI CTCGAG 1 cut(s) 82
SmiMI CAYNNNNRTG 2 cut(s) 794, 980
SmlI CTYRAG 3 cut(s) 82, 134, 1028
SmoI CTYRAG 3 cut(s) 82, 134, 1028
Sse9I AATT 5 cut(s) 284, 360, 767, 938, 971
SsiI CCGC 2 cut(s) 235, 1081
StyD4I CCNGG 2 cut(s) 99, 387
TaaI ACNGT 4 cut(s) 373, 385, 418, 620
TaqI TCGA 6 cut(s) 8, 17, 83, 489, 701, 1171
TasI AATT 5 cut(s) 284, 360, 767, 938, 971
TatI WGTACW 1 cut(s) 951
TauI GCSGC 1 cut(s) 238
TfiI GAWTC 2 cut(s) 269, 725
Tru1I TTAA 6 cut(s) 186, 363, 770, 825, 890, 1205
Tru9I TTAA 6 cut(s) 186, 363, 770, 825, 890, 1205
TscAI CASTG 4 cut(s) 100, 421, 625, 1146
TseFI GTSAC 4 cut(s) 193, 412, 473, 812
TseI GCWGC 7 cut(s) 38, 46, 173, 332, 439, 707, 710
Tsp45I GTSAC 4 cut(s) 193, 412, 473, 812
TspDTI ATGAA 3 cut(s) 528, 1056, 1149
TspRI CASTG 4 cut(s) 100, 421, 625, 1146
Van91I CCANNNNNTGG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 70
XagI CCTNNNNNAGG 2 cut(s) 392, 611
XapI RAATTY 1 cut(s) 938
XceI RCATGY 1 cut(s) 1052
XhoI CTCGAG 1 cut(s) 82
XmnI GAANNNNTTC 1 cut(s) 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.