FvH4_4g35250

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016191
Physical Location & Seq
Forward (+)
32608958 .. 32609984
1027 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35250.t1

Sequence Viewer

Length: 666 bp
ATGAGAAAGAACTCTTCATTTTTCCATCTATTCCTCAGCTTGGCTCTTCTACTGGGCCAAGCCAAACCCGACCCGGATCCACTTCAAGACTACTGCATAGCAGATACCATAACCCCTGGCTCATTTTACTTCAATGGTGCACCCTGCATTAATCCAAAGCATGCACTCCCCTCCCATTTCACCACATCAGTTCTATCCAAGCCAGGAAACACAAAGGCCAACCCTTTCGGCTTCAACGTAACTCTAACCAACACGCTCAACTTACCAGGCCTCAACACCTTGGGCTTGACCATGGCCCGAGTTGACATAGCAGCCAATGGGCTTGTCCCTCCACACTTACACCCACGGGCCTCGGAGGTAACCACCTGCCTCAAGGGCCTGCTTCTTGTGGGCTTTGTGGACACATCTAACCGTGTTTTCACCCAACAATTAAGGCCTGGTGAGTCATTTGTCTTCCCAAAGTCTCTCATTCATTTTCTGTACAACTATGATTCAAGGGCTCCGGCAGTGGCATTGTCCGGTTTGAGTAGCCAGAACCCTGGTGCACAGATTGTGTCACTTGCCACATTCACTTCAAACCCTCCAATTGACAATGAGATACTGATGAAAGGTTTTCAAATTACGGATCGAGACGTTGCCAGAATCCGAAAGAACCTAGGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

222

Amino Acids

23.92

Weight (kDa)

9.27

Isoelectric Point (pI)

22.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 65 - 210 6.3e-43 Cupin
Cupin_2 PF07883 100 - 172 2.5e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10460
fragaria_vesca FvH4_4g35250
malus_domestica MD16G1084100.v1.1
prunus_persica Prupe.1G261900_v2.0.a1
pyrus_communis pycom16g07310
rosa_chinensis RchiOBHm_Chr4g0444861
rosa_laevigata RLG00000005794
rosa_multiflora Rmu_sc0006845.1_g000007
rosa_roxburghii Rroxscaffold_5G00385350
rosa_rugosa Rorug04G0355700
rosa_samantha Rh4AG417200 Rh4BG429000 Rh4CG443700 Rh4DG424900
rosa_wichuraiana Rw4G035760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 374
Acc36I ACCTGC 1 cut(s) 374
AclWI GGATC 3 cut(s) 71, 84, 633
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 1 cut(s) 40
AgsI TTSAA 6 cut(s) 86, 133, 235, 495, 576, 617
AjnI CCWGG 5 cut(s) 115, 202, 265, 436, 538
AluBI AGCT 1 cut(s) 39
AluI AGCT 1 cut(s) 39
Alw21I GWGCWC 2 cut(s) 142, 547
Alw26I GTCTC 2 cut(s) 468, 624
Alw44I GTGCAC 2 cut(s) 138, 543
AlwI GGATC 3 cut(s) 71, 84, 633
Ama87I CYCGRG 1 cut(s) 297
AoxI GGCC 7 cut(s) 55, 216, 268, 294, 348, 376, 434
ApaLI GTGCAC 2 cut(s) 138, 543
ApeKI GCWGC 1 cut(s) 311
AseI ATTAAT 1 cut(s) 150
AspA2I CCTAGG 1 cut(s) 655
AspS9I GGNCC 4 cut(s) 55, 295, 348, 376
AsuC2I CCSGG 1 cut(s) 74
AsuHPI GGTGA 3 cut(s) 172, 412, 452
AvaI CYCGRG 1 cut(s) 297
AvrII CCTAGG 1 cut(s) 655
BaeGI GKGCMC 2 cut(s) 142, 547
BamHI GGATCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 502
BbsI GAAGAC 1 cut(s) 445
Bbv12I GWGCWC 2 cut(s) 142, 547
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 323
BccI CCATC 1 cut(s) 33
BciT130I CCWGG 5 cut(s) 117, 204, 267, 438, 540
BcnI CCSGG 1 cut(s) 74
BcoDI GTCTC 2 cut(s) 468, 624
BfaI CTAG 1 cut(s) 656
BfuAI ACCTGC 1 cut(s) 374
BglI GCCNNNNNGGC 1 cut(s) 375
BisI GCNGC 1 cut(s) 312
BlnI CCTAGG 1 cut(s) 655
BlsI GCNGC 1 cut(s) 313
Bme1390I CCNGG 6 cut(s) 74, 117, 204, 267, 438, 540
BmeT110I CYCGRG 1 cut(s) 297
BmgT120I GGNCC 4 cut(s) 55, 295, 348, 376
BmiI GGNNCC 2 cut(s) 78, 501
BmrFI CCNGG 6 cut(s) 74, 117, 204, 267, 438, 540
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 445
Bpu10I CCTNAGC 1 cut(s) 35
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 74
BsaJI CCNNGG 7 cut(s) 115, 279, 291, 344, 351, 538, 655
BsaWI WCCGGW 1 cut(s) 518
Bsc4I CCNNNNNNNGG 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 57
BseBI CCWGG 5 cut(s) 117, 204, 267, 438, 540
BseDI CCNNGG 7 cut(s) 115, 279, 291, 344, 351, 538, 655
BseLI CCNNNNNNNGG 1 cut(s) 40
BseMII CTCAG 1 cut(s) 49
BseNI ACTGG 1 cut(s) 57
BseSI GKGCMC 2 cut(s) 142, 547
BseXI GCAGC 1 cut(s) 323
BshFI GGCC 7 cut(s) 57, 218, 270, 296, 350, 378, 436
BsiHKAI GWGCWC 2 cut(s) 142, 547
BsiHKCI CYCGRG 1 cut(s) 297
BsiSI CCGG 3 cut(s) 74, 503, 519
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 1 cut(s) 40
BsmAI GTCTC 2 cut(s) 468, 624
BsmBI CGTCTC 1 cut(s) 624
BsmFI GGGAC 1 cut(s) 311
BsnI GGCC 7 cut(s) 57, 218, 270, 296, 350, 378, 436
BsoBI CYCGRG 1 cut(s) 297
Bsp1286I GDGCHC 3 cut(s) 142, 502, 547
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 2 cut(s) 76, 625
Bsp19I CCATGG 1 cut(s) 291
BspANI GGCC 7 cut(s) 57, 218, 270, 296, 350, 378, 436
BspCNI CTCAG 1 cut(s) 48
BspLI GGNNCC 2 cut(s) 78, 501
BspMI ACCTGC 1 cut(s) 374
BspPI GGATC 3 cut(s) 71, 84, 633
BspQI GCTCTTC 1 cut(s) 51
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 1 cut(s) 57
BssECI CCNNGG 7 cut(s) 115, 279, 291, 344, 351, 538, 655
BssMI GATC 2 cut(s) 76, 625
BssT1I CCWWGG 3 cut(s) 279, 291, 655
Bst2UI CCWGG 5 cut(s) 117, 204, 267, 438, 540
Bst4CI ACNGT 1 cut(s) 413
Bst6I CTCTTC 2 cut(s) 19, 51
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 162, 380
BstDEI CTNAG 1 cut(s) 35
BstDSI CCRYGG 2 cut(s) 291, 344
BstEII GGTNACC 1 cut(s) 358
BstKTI GATC 2 cut(s) 79, 628
BstMAI GTCTC 2 cut(s) 468, 624
BstMBI GATC 2 cut(s) 76, 625
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstNI CCWGG 5 cut(s) 117, 204, 267, 438, 540
BstNSI RCATGY 1 cut(s) 164
BstPI GGTNACC 1 cut(s) 358
BstSCI CCNGG 6 cut(s) 72, 115, 202, 265, 436, 538
BstSLI GKGCMC 2 cut(s) 142, 547
BstV1I GCAGC 1 cut(s) 323
BstV2I GAAGAC 1 cut(s) 445
BstX2I RGATCY 1 cut(s) 76
BstXI CCANNNNNNTGG 1 cut(s) 539
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 7 cut(s) 57, 218, 270, 296, 350, 378, 436
BtgI CCRYGG 2 cut(s) 291, 344
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 1 cut(s) 513
BveI ACCTGC 1 cut(s) 374
Cac8I GCNNGC 2 cut(s) 162, 380
Cfr13I GGNCC 4 cut(s) 55, 295, 348, 376
Csp6I GTAC 1 cut(s) 481
CviAII CATG 2 cut(s) 161, 292
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 1 cut(s) 35
DpnI GATC 2 cut(s) 78, 627
DpnII GATC 2 cut(s) 76, 625
Eam1104I CTCTTC 2 cut(s) 19, 51
EarI CTCTTC 2 cut(s) 19, 51
Eco130I CCWWGG 3 cut(s) 279, 291, 655
Eco147I AGGCCT 2 cut(s) 270, 436
Eco24I GRGCYC 1 cut(s) 502
Eco88I CYCGRG 1 cut(s) 297
Eco91I GGTNACC 1 cut(s) 358
EcoO109I RGGNCCY 1 cut(s) 376
EcoO65I GGTNACC 1 cut(s) 358
EcoRII CCWGG 5 cut(s) 115, 202, 265, 436, 538
EcoT14I CCWWGG 3 cut(s) 279, 291, 655
EcoT38I GRGCYC 1 cut(s) 502
ErhI CCWWGG 3 cut(s) 279, 291, 655
Esp3I CGTCTC 1 cut(s) 624
FaeI CATG 2 cut(s) 164, 295
FaiI YATR 6 cut(s) 98, 110, 162, 293, 308, 489
FaqI GGGAC 1 cut(s) 311
FatI CATG 2 cut(s) 160, 291
Fnu4HI GCNGC 1 cut(s) 312
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 1 cut(s) 656
GluI GCNGC 1 cut(s) 312
HaeIII GGCC 7 cut(s) 57, 218, 270, 296, 350, 378, 436
HapII CCGG 3 cut(s) 74, 503, 519
Hin1II CATG 2 cut(s) 164, 295
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 3 cut(s) 443, 491, 642
HpaII CCGG 3 cut(s) 74, 503, 519
HphI GGTGA 3 cut(s) 172, 412, 452
Hpy166II GTNNAC 4 cut(s) 140, 304, 400, 545
Hpy188I TCNGA 2 cut(s) 355, 647
Hpy188III TCNNGA 2 cut(s) 86, 629
Hpy8I GTNNAC 4 cut(s) 140, 304, 400, 545
HpyCH4III ACNGT 1 cut(s) 413
HpyCH4IV ACGT 2 cut(s) 237, 633
HpyCH4V TGCA 5 cut(s) 96, 140, 147, 164, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 2 cut(s) 237, 633
Hsp92II CATG 2 cut(s) 164, 295
Kzo9I GATC 2 cut(s) 76, 625
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 505
Lsp1109I GCAGC 1 cut(s) 323
MaeI CTAG 1 cut(s) 656
MaeII ACGT 2 cut(s) 237, 633
MaeIII GTNAC 3 cut(s) 238, 358, 555
MalI GATC 2 cut(s) 78, 627
MboI GATC 2 cut(s) 76, 625
MboII GAAGA 3 cut(s) 6, 38, 445
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 3 cut(s) 142, 502, 547
MluCI AATT 3 cut(s) 428, 585, 618
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 9 cut(s) 44, 181, 281, 339, 349, 361, 380, 591, 653
MseI TTAA 3 cut(s) 150, 431, 664
MspI CCGG 3 cut(s) 74, 503, 519
MspR9I CCNGG 6 cut(s) 74, 117, 204, 267, 438, 540
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 5 cut(s) 117, 204, 267, 438, 540
MwoI GCNNNNNNNGC 1 cut(s) 375
NciI CCSGG 1 cut(s) 74
NcoI CCATGG 1 cut(s) 291
NdeII GATC 2 cut(s) 76, 625
NlaIII CATG 2 cut(s) 164, 295
NlaIV GGNNCC 2 cut(s) 78, 501
NmuCI GTSAC 1 cut(s) 555
NspI RCATGY 1 cut(s) 164
PaeI GCATGC 1 cut(s) 164
PaqCI CACCTGC 1 cut(s) 374
PceI AGGCCT 2 cut(s) 270, 436
PciSI GCTCTTC 1 cut(s) 51
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PfeI GAWTC 2 cut(s) 491, 642
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PshBI ATTAAT 1 cut(s) 150
Psp6I CCWGG 5 cut(s) 115, 202, 265, 436, 538
PspEI GGTNACC 1 cut(s) 358
PspGI CCWGG 5 cut(s) 115, 202, 265, 436, 538
PspN4I GGNNCC 2 cut(s) 78, 501
PspPI GGNCC 4 cut(s) 55, 295, 348, 376
PsuI RGATCY 1 cut(s) 76
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 3 cut(s) 150, 431, 664
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 2 cut(s) 76, 625
Sau96I GGNCC 4 cut(s) 55, 295, 348, 376
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 6 cut(s) 74, 117, 204, 267, 438, 540
SduI GDGCHC 3 cut(s) 142, 502, 547
SetI ASST 9 cut(s) 41, 240, 281, 360, 368, 613, 636, 657, 664
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SphI GCATGC 1 cut(s) 164
Sse9I AATT 3 cut(s) 428, 585, 618
SseBI AGGCCT 2 cut(s) 270, 436
SspMI CTAG 1 cut(s) 656
StuI AGGCCT 2 cut(s) 270, 436
StyD4I CCNGG 6 cut(s) 72, 115, 202, 265, 436, 538
StyI CCWWGG 3 cut(s) 279, 291, 655
TaaI ACNGT 1 cut(s) 413
TaiI ACGT 2 cut(s) 240, 636
TaqI TCGA 1 cut(s) 628
TasI AATT 3 cut(s) 428, 585, 618
TatI WGTACW 1 cut(s) 480
TfiI GAWTC 2 cut(s) 491, 642
Tru1I TTAA 3 cut(s) 150, 431, 664
Tru9I TTAA 3 cut(s) 150, 431, 664
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 1 cut(s) 555
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 1 cut(s) 555
TspDTI ATGAA 3 cut(s) 6, 461, 620
TspGWI ACGGA 1 cut(s) 638
TspRI CASTG 1 cut(s) 513
VneI GTGCAC 2 cut(s) 138, 543
VspI ATTAAT 1 cut(s) 150
XceI RCATGY 1 cut(s) 164
XmaJI CCTAGG 1 cut(s) 655
XspI CTAG 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.