RLG00000005794

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0016191
Physical Location & Seq
Reverse (-)
1807565 .. 1808857
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005794

Sequence Viewer

Length: 831 bp
ATGTCCCACATTTGCTATATAACTCCAGTTTTGCTCACTGCAAATCTCAACCCAACCTTCTCTGTTGATGTAAAGAAAGGCAGAATGAGAAAGAACTCTTCCTTTCTCCATTTATTCCTCAGCTTTGCTCTTCTACTGGGCCAAGCCAAACCCGACCCAGATCCACTTCAAGACTACTGCATAGCAGATACTAAAACCCCTCAGTATGTTTACTTCAACGGTGCACCCTGCATTAATCCAAAGCTAGCAGCCTCCTCCCATTTCTCTACATCAGTCCTATCCAAACCCGGAAACACTAAAGCCAACCCTTTCGGCTTCAATGTAACTCTAACCAACACGGACAATTTACCGGGCATGAACACCTTGGGCTTGAGCATGGCCCGAGTTGACATAGATGCTAATGGGATTGTCCCTCCGCACACACACCCACGGGCCTCAGAGGTAACCATCTGCCTCAAGGGCCGGCTTCTTGTGGGCTTTGTGGACACATCTAACCGTGGTTTCACCCAACAATTAAACCCTGGTGAGTCATTTGTTTTTCCAAAGGGTCTCATTCATTTTCTGTACAACTATGATTCAAAGGCTCCGGCAGTGGCATTGTCCGGCTTGAGTAGCCAAAACCCTGGTGCACAGATTGTGTCTCTAGCCTCATTCACTTCAAAGCCTCCAATTGAGATTCAGATATTGGAAAAAAGTTTTCAAATTACCGATCAAGACGTTGCCAGGATCCGAAAGAACCTCGGAGGGAAAGAACTTTCTGGTACTGCAATTAGCTTCCTCCAGCAAAGCCATTCCTTCAAGCAACTTCGTTATAAAAGTCCAATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

277

Amino Acids

30.12

Weight (kDa)

9.43

Isoelectric Point (pI)

25.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 93 - 239 4e-44 Cupin
Cupin_2 PF07883 128 - 200 4.5e-10 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10460
fragaria_vesca FvH4_4g35250
malus_domestica MD16G1084100.v1.1
prunus_persica Prupe.1G261900_v2.0.a1
pyrus_communis pycom16g07310
rosa_chinensis RchiOBHm_Chr4g0444861
rosa_laevigata RLG00000005794
rosa_multiflora Rmu_sc0006845.1_g000007
rosa_roxburghii Rroxscaffold_5G00385350
rosa_rugosa Rorug04G0355700
rosa_samantha Rh4AG417200 Rh4BG429000 Rh4CG443700 Rh4DG424900
rosa_wichuraiana Rw4G035760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 813
AciI CCGC 1 cut(s) 416
AclWI GGATC 3 cut(s) 155, 721, 734
AfaI GTAC 2 cut(s) 566, 763
AgsI TTSAA 7 cut(s) 170, 217, 319, 579, 660, 701, 799
AjnI CCWGG 3 cut(s) 520, 622, 722
AluBI AGCT 3 cut(s) 123, 244, 774
AluI AGCT 3 cut(s) 123, 244, 774
Alw21I GWGCWC 2 cut(s) 226, 631
Alw26I GTCTC 2 cut(s) 554, 645
Alw44I GTGCAC 2 cut(s) 222, 627
AlwI GGATC 3 cut(s) 155, 721, 734
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 4 cut(s) 139, 378, 432, 460
ApaLI GTGCAC 2 cut(s) 222, 627
ApeKI GCWGC 1 cut(s) 248
AseI ATTAAT 1 cut(s) 234
AspS9I GGNCC 4 cut(s) 139, 379, 432, 460
AsuC2I CCSGG 2 cut(s) 288, 351
AsuHPI GGTGA 2 cut(s) 496, 536
AsuNHI GCTAGC 1 cut(s) 244
AvaI CYCGRG 1 cut(s) 381
BaeGI GKGCMC 2 cut(s) 226, 631
BamHI GGATCC 1 cut(s) 726
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
Bbv12I GWGCWC 2 cut(s) 226, 631
BbvCI CCTCAGC 1 cut(s) 119
BbvI GCAGC 1 cut(s) 260
BccI CCATC 1 cut(s) 455
BciT130I CCWGG 3 cut(s) 522, 624, 724
BcnI CCSGG 2 cut(s) 288, 351
BcoDI GTCTC 2 cut(s) 554, 645
BfaI CTAG 2 cut(s) 245, 644
BglI GCCNNNNNGGC 1 cut(s) 459
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
Bme1390I CCNGG 5 cut(s) 288, 351, 522, 624, 724
BmeT110I CYCGRG 1 cut(s) 381
BmgT120I GGNCC 4 cut(s) 139, 379, 432, 460
BmiI GGNNCC 2 cut(s) 585, 728
BmrFI CCNGG 5 cut(s) 288, 351, 522, 624, 724
BmrI ACTGGG 1 cut(s) 146
BmsI GCATC 1 cut(s) 385
BmtI GCTAGC 1 cut(s) 248
BmuI ACTGGG 1 cut(s) 146
BpmI CTGGAG 2 cut(s) 9, 764
Bpu10I CCTNAGC 1 cut(s) 119
BpuEI CTTGAG 3 cut(s) 391, 440, 628
BpuMI CCSGG 2 cut(s) 288, 351
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 6 cut(s) 363, 428, 496, 520, 622, 739
Bse118I RCCGGY 1 cut(s) 462
Bse1I ACTGG 2 cut(s) 26, 141
BseBI CCWGG 3 cut(s) 522, 624, 724
BseDI CCNNGG 6 cut(s) 363, 428, 496, 520, 622, 739
BseMII CTCAG 3 cut(s) 133, 215, 450
BseNI ACTGG 2 cut(s) 26, 141
BseRI GAGGAG 1 cut(s) 244
BseSI GKGCMC 2 cut(s) 226, 631
BseXI GCAGC 1 cut(s) 260
BshFI GGCC 4 cut(s) 141, 380, 434, 462
BsiHKAI GWGCWC 2 cut(s) 226, 631
BsiHKCI CYCGRG 1 cut(s) 381
BsiSI CCGG 5 cut(s) 288, 350, 463, 587, 603
BslFI GGGAC 1 cut(s) 395
BsmAI GTCTC 2 cut(s) 554, 645
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 4 cut(s) 141, 380, 434, 462
Bso31I GGTCTC 1 cut(s) 554
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 226, 631
Bsp1407I TGTACA 1 cut(s) 564
Bsp143I GATC 3 cut(s) 160, 709, 726
BspACI CCGC 1 cut(s) 416
BspANI GGCC 4 cut(s) 141, 380, 434, 462
BspCNI CTCAG 3 cut(s) 132, 214, 449
BspLI GGNNCC 2 cut(s) 585, 728
BspOI GCTAGC 1 cut(s) 248
BspPI GGATC 3 cut(s) 155, 721, 734
BspQI GCTCTTC 1 cut(s) 135
BspTNI GGTCTC 1 cut(s) 554
BsrFI RCCGGY 1 cut(s) 462
BsrGI TGTACA 1 cut(s) 564
BsrI ACTGG 2 cut(s) 26, 141
BssAI RCCGGY 1 cut(s) 462
BssECI CCNNGG 6 cut(s) 363, 428, 496, 520, 622, 739
BssMI GATC 3 cut(s) 160, 709, 726
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 3 cut(s) 522, 624, 724
Bst4CI ACNGT 2 cut(s) 221, 497
Bst6I CTCTTC 2 cut(s) 103, 135
BstAUI TGTACA 1 cut(s) 564
BstC8I GCNNGC 2 cut(s) 246, 464
BstDEI CTNAG 3 cut(s) 119, 201, 436
BstDSI CCRYGG 2 cut(s) 428, 496
BstEII GGTNACC 1 cut(s) 442
BstKTI GATC 3 cut(s) 163, 712, 729
BstMAI GTCTC 2 cut(s) 554, 645
BstMBI GATC 3 cut(s) 160, 709, 726
BstMWI GCNNNNNNNGC 2 cut(s) 459, 612
BstNI CCWGG 3 cut(s) 522, 624, 724
BstPI GGTNACC 1 cut(s) 442
BstSCI CCNGG 5 cut(s) 286, 349, 520, 622, 722
BstSLI GKGCMC 2 cut(s) 226, 631
BstV1I GCAGC 1 cut(s) 260
BstX2I RGATCY 2 cut(s) 160, 726
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 2 cut(s) 160, 726
BsuRI GGCC 4 cut(s) 141, 380, 434, 462
BtgI CCRYGG 2 cut(s) 428, 496
BtsI GCAGTG 2 cut(s) 36, 597
BtsIMutI CAGTG 2 cut(s) 36, 597
Cac8I GCNNGC 2 cut(s) 246, 464
Cfr10I RCCGGY 1 cut(s) 462
Cfr13I GGNCC 4 cut(s) 139, 379, 432, 460
Csp6I GTAC 2 cut(s) 565, 762
CviAII CATG 3 cut(s) 355, 376, 826
CviQI GTAC 2 cut(s) 565, 762
DdeI CTNAG 3 cut(s) 119, 201, 436
DpnI GATC 3 cut(s) 162, 711, 728
DpnII GATC 3 cut(s) 160, 709, 726
Eam1104I CTCTTC 2 cut(s) 103, 135
EarI CTCTTC 2 cut(s) 103, 135
Eco130I CCWWGG 1 cut(s) 363
Eco31I GGTCTC 1 cut(s) 554
Eco88I CYCGRG 1 cut(s) 381
Eco91I GGTNACC 1 cut(s) 442
EcoO65I GGTNACC 1 cut(s) 442
EcoRII CCWGG 3 cut(s) 520, 622, 722
EcoT14I CCWWGG 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 3 cut(s) 358, 379, 829
FaqI GGGAC 1 cut(s) 395
FatI CATG 3 cut(s) 354, 375, 825
Fnu4HI GCNGC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 249
FspBI CTAG 2 cut(s) 245, 644
GluI GCNGC 1 cut(s) 249
GsuI CTGGAG 2 cut(s) 9, 764
HaeIII GGCC 4 cut(s) 141, 380, 434, 462
HapII CCGG 5 cut(s) 288, 350, 463, 587, 603
Hin1II CATG 3 cut(s) 358, 379, 829
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 3 cut(s) 527, 575, 676
HpaII CCGG 5 cut(s) 288, 350, 463, 587, 603
HphI GGTGA 2 cut(s) 496, 536
Hpy166II GTNNAC 5 cut(s) 211, 224, 388, 484, 629
Hpy188I TCNGA 4 cut(s) 439, 681, 731, 743
Hpy188III TCNNGA 2 cut(s) 170, 713
Hpy8I GTNNAC 5 cut(s) 211, 224, 388, 484, 629
HpyAV CCTTC 2 cut(s) 67, 805
HpyCH4III ACNGT 2 cut(s) 221, 497
HpyCH4IV ACGT 1 cut(s) 717
HpyCH4V TGCA 6 cut(s) 41, 180, 224, 231, 629, 767
HpyF10VI GCNNNNNNNGC 2 cut(s) 459, 612
HpyF3I CTNAG 3 cut(s) 119, 201, 436
HpySE526I ACGT 1 cut(s) 717
Hsp92II CATG 3 cut(s) 358, 379, 829
KroI GCCGGC 1 cut(s) 462
KroNI GCCGGC 1 cut(s) 464
Kzo9I GATC 3 cut(s) 160, 709, 726
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 589
Lsp1109I GCAGC 1 cut(s) 260
LweI GCATC 1 cut(s) 385
MaeI CTAG 2 cut(s) 245, 644
MaeII ACGT 1 cut(s) 717
MaeIII GTNAC 2 cut(s) 322, 442
MalI GATC 3 cut(s) 162, 711, 728
MboI GATC 3 cut(s) 160, 709, 726
MboII GAAGA 2 cut(s) 90, 122
MfeI CAATTG 1 cut(s) 669
MflI RGATCY 2 cut(s) 160, 726
MhlI GDGCHC 2 cut(s) 226, 631
MluCI AATT 5 cut(s) 343, 512, 669, 702, 768
MlyI GAGTC 1 cut(s) 536
MroNI GCCGGC 1 cut(s) 462
MseI TTAA 2 cut(s) 234, 515
MspI CCGG 5 cut(s) 288, 350, 463, 587, 603
MspR9I CCNGG 5 cut(s) 288, 351, 522, 624, 724
MunI CAATTG 1 cut(s) 669
MvaI CCWGG 3 cut(s) 522, 624, 724
MwoI GCNNNNNNNGC 2 cut(s) 459, 612
NaeI GCCGGC 1 cut(s) 464
NciI CCSGG 2 cut(s) 288, 351
NdeII GATC 3 cut(s) 160, 709, 726
NgoMIV GCCGGC 1 cut(s) 462
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 3 cut(s) 358, 379, 829
NlaIV GGNNCC 2 cut(s) 585, 728
PciSI GCTCTTC 1 cut(s) 135
PdiI GCCGGC 1 cut(s) 464
PfeI GAWTC 2 cut(s) 575, 676
PkrI GCNGC 1 cut(s) 250
PleI GAGTC 1 cut(s) 535
PpsI GAGTC 1 cut(s) 535
PshBI ATTAAT 1 cut(s) 234
PsiI TTATAA 1 cut(s) 813
Psp6I CCWGG 3 cut(s) 520, 622, 722
PspEI GGTNACC 1 cut(s) 442
PspGI CCWGG 3 cut(s) 520, 622, 722
PspN4I GGNNCC 2 cut(s) 585, 728
PspPI GGNCC 4 cut(s) 139, 379, 432, 460
PsuI RGATCY 2 cut(s) 160, 726
RsaI GTAC 2 cut(s) 566, 763
RsaNI GTAC 2 cut(s) 565, 762
SapI GCTCTTC 1 cut(s) 135
SaqAI TTAA 2 cut(s) 234, 515
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 3 cut(s) 160, 709, 726
Sau96I GGNCC 4 cut(s) 139, 379, 432, 460
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 5 cut(s) 288, 351, 522, 624, 724
SduI GDGCHC 2 cut(s) 226, 631
SetI ASST 8 cut(s) 59, 125, 246, 365, 444, 720, 741, 776
SfaNI GCATC 1 cut(s) 385
SmlI CTYRAG 3 cut(s) 370, 455, 607
SmoI CTYRAG 3 cut(s) 370, 455, 607
Sse9I AATT 5 cut(s) 343, 512, 669, 702, 768
SsiI CCGC 1 cut(s) 416
SspMI CTAG 2 cut(s) 245, 644
StyD4I CCNGG 5 cut(s) 286, 349, 520, 622, 722
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 2 cut(s) 221, 497
TaiI ACGT 1 cut(s) 720
TasI AATT 5 cut(s) 343, 512, 669, 702, 768
TatI WGTACW 1 cut(s) 564
TfiI GAWTC 2 cut(s) 575, 676
Tru1I TTAA 2 cut(s) 234, 515
Tru9I TTAA 2 cut(s) 234, 515
TscAI CASTG 2 cut(s) 43, 597
TseI GCWGC 1 cut(s) 248
TspDTI ATGAA 2 cut(s) 371, 545
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 2 cut(s) 43, 597
VneI GTGCAC 2 cut(s) 222, 627
VspI ATTAAT 1 cut(s) 234
XspI CTAG 2 cut(s) 245, 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.