pycom16g07310

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0016191
Physical Location & Seq
Reverse (-)
4774925 .. 4775584
660 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g07310.1

Sequence Viewer

Length: 660 bp
ATGAACACAATCAATTATCTCTTTCTCCAATTATTCCTCAACCTAGCTCTATTCGGCCGCACCAAATCCGACCCGGATCCGCTCCAGGACTACTGCATAGCGGATCCGGAAACCCCTCAGGAATTTTCCCTCAATGGTGCACCATGCATTGACCCAAACCTAGCAACCTCTTCCCACTTCACCACATCAGCCCTATCCAAACCAGGCGACACTAAAGCCAACGGGTTCAACGTGACCCTCACCAACCGGTTCAATCTGCCCGGGCTCAACACATTGGGCCTGACCATGGCCCGAGTTGACATAGATGCCAATGGGATTGTGCCACCTCACTTGCATCCAAGGGCCTCGGAGGTCACAATCTGCCTCACGGGCTTCCTTCTTGTGGGCTTTGTGGACACATCAAACCGGGTCTTTACCCAGCAACTGAGGCCCGGGGAGTCTTTTGTGTTCCCCAAGGGTTTGATACATTTTCTGTACAACTACGACTCGTTGGAACCAGCCGTGGCATTGGCCGGGCTCGGTAGCCAAAACCCTGGTGCACAGATTGCGTCCATGGCCTCGTTCACATCCGAGCCTTCAAACCCCGTCGAGATTTTGAAGAAAGGGTTTCAGATTAGTGACCTAAACGCTGCCTGGATTCGGAGGAACCTCGGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

220

Amino Acids

23.71

Weight (kDa)

5.48

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 65 - 207 1.3e-38 Cupin
Cupin_2 PF07883 98 - 170 2.3e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10460
fragaria_vesca FvH4_4g35250
malus_domestica MD16G1084100.v1.1
prunus_persica Prupe.1G261900_v2.0.a1
pyrus_communis pycom16g07310
rosa_chinensis RchiOBHm_Chr4g0444861
rosa_laevigata RLG00000005794
rosa_multiflora Rmu_sc0006845.1_g000007
rosa_roxburghii Rroxscaffold_5G00385350
rosa_rugosa Rorug04G0355700
rosa_samantha Rh4AG417200 Rh4BG429000 Rh4CG443700 Rh4DG424900
rosa_wichuraiana Rw4G035760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 82
AccIII TCCGGA 1 cut(s) 106
AciI CCGC 3 cut(s) 58, 80, 101
AclWI GGATC 4 cut(s) 71, 84, 98, 111
AcoI YGGCCR 2 cut(s) 55, 510
AcsI RAATTY 1 cut(s) 122
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 3 cut(s) 286, 382, 639
AgeI ACCGGT 1 cut(s) 246
AgsI TTSAA 4 cut(s) 229, 253, 579, 598
AjnI CCWGG 4 cut(s) 84, 202, 532, 632
AluBI AGCT 1 cut(s) 47
AluI AGCT 1 cut(s) 47
Alw21I GWGCWC 2 cut(s) 142, 541
Alw44I GTGCAC 2 cut(s) 138, 537
AlwI GGATC 4 cut(s) 71, 84, 98, 111
AlwNI CAGNNNCTG 1 cut(s) 424
Ama87I CYCGRG 3 cut(s) 260, 291, 431
Aor13HI TCCGGA 1 cut(s) 106
AoxI GGCC 7 cut(s) 55, 277, 288, 342, 428, 510, 555
ApaLI GTGCAC 2 cut(s) 138, 537
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 1 cut(s) 122
AsiGI ACCGGT 1 cut(s) 246
AspS9I GGNCC 4 cut(s) 277, 289, 342, 429
AsuC2I CCSGG 7 cut(s) 74, 261, 262, 407, 432, 433, 514
AsuHPI GGTGA 2 cut(s) 172, 232
AvaI CYCGRG 3 cut(s) 260, 291, 431
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 2 cut(s) 142, 541
BamHI GGATCC 2 cut(s) 76, 103
BanII GRGCYC 2 cut(s) 267, 519
Bbv12I GWGCWC 2 cut(s) 142, 541
BbvI GCAGC 1 cut(s) 616
BceAI ACGGC 1 cut(s) 485
BciT130I CCWGG 4 cut(s) 86, 204, 534, 634
BcnI CCSGG 7 cut(s) 74, 261, 262, 407, 432, 433, 514
BfaI CTAG 2 cut(s) 44, 161
BglI GCCNNNNNGGC 1 cut(s) 369
BisI GCNGC 2 cut(s) 58, 630
BlsI GCNGC 2 cut(s) 59, 631
BmeT110I CYCGRG 3 cut(s) 260, 291, 431
BmgT120I GGNCC 4 cut(s) 277, 289, 342, 429
BmiI GGNNCC 4 cut(s) 78, 105, 495, 647
BmsI GCATC 2 cut(s) 295, 343
BpmI CTGGAG 1 cut(s) 68
BpuMI CCSGG 7 cut(s) 74, 261, 262, 407, 432, 433, 514
BsaWI WCCGGW 2 cut(s) 106, 246
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 382, 639
Bse118I RCCGGY 1 cut(s) 246
Bse21I CCTNAGG 1 cut(s) 117
BseAI TCCGGA 1 cut(s) 106
BseBI CCWGG 4 cut(s) 86, 204, 534, 634
BseGI GGATG 2 cut(s) 334, 566
BseLI CCNNNNNNNGG 3 cut(s) 286, 382, 639
BseMII CTCAG 2 cut(s) 131, 416
BseSI GKGCMC 2 cut(s) 142, 541
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 1 cut(s) 616
BseYI CCCAGC 1 cut(s) 417
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 7 cut(s) 57, 279, 290, 344, 430, 512, 557
BshTI ACCGGT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 2 cut(s) 142, 541
BsiHKCI CYCGRG 3 cut(s) 260, 291, 431
BsiSI CCGG 7 cut(s) 74, 107, 247, 261, 406, 432, 513
BslI CCNNNNNNNGG 3 cut(s) 286, 382, 639
BsnI GGCC 7 cut(s) 57, 279, 290, 344, 430, 512, 557
BsoBI CYCGRG 3 cut(s) 260, 291, 431
Bsp1286I GDGCHC 4 cut(s) 142, 267, 519, 541
Bsp13I TCCGGA 1 cut(s) 106
Bsp1407I TGTACA 1 cut(s) 474
Bsp143I GATC 2 cut(s) 76, 103
Bsp19I CCATGG 2 cut(s) 285, 552
BspACI CCGC 3 cut(s) 58, 80, 101
BspANI GGCC 7 cut(s) 57, 279, 290, 344, 430, 512, 557
BspCNI CTCAG 2 cut(s) 130, 417
BspEI TCCGGA 1 cut(s) 106
BspLI GGNNCC 4 cut(s) 78, 105, 495, 647
BspPI GGATC 4 cut(s) 71, 84, 98, 111
BsrBI CCGCTC 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 246
BsrGI TGTACA 1 cut(s) 474
BssAI RCCGGY 1 cut(s) 246
BssMI GATC 2 cut(s) 76, 103
BssT1I CCWWGG 4 cut(s) 285, 338, 453, 552
Bst2UI CCWGG 4 cut(s) 86, 204, 534, 634
Bst6I CTCTTC 1 cut(s) 175
BstAPI GCANNNNNTGC 1 cut(s) 545
BstAUI TGTACA 1 cut(s) 474
BstDEI CTNAG 2 cut(s) 117, 425
BstDSI CCRYGG 3 cut(s) 285, 501, 552
BstF5I GGATG 2 cut(s) 334, 566
BstKTI GATC 2 cut(s) 79, 106
BstMBI GATC 2 cut(s) 76, 103
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 4 cut(s) 369, 427, 545, 554
BstNI CCWGG 4 cut(s) 86, 204, 534, 634
BstSLI GKGCMC 2 cut(s) 142, 541
BstV1I GCAGC 1 cut(s) 616
BstX2I RGATCY 2 cut(s) 76, 103
BstXI CCANNNNNNTGG 1 cut(s) 533
BstYI RGATCY 2 cut(s) 76, 103
BstZI CGGCCG 1 cut(s) 55
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 7 cut(s) 57, 279, 290, 344, 430, 512, 557
BtgI CCRYGG 3 cut(s) 285, 501, 552
BtsCI GGATG 2 cut(s) 334, 566
CaiI CAGNNNCTG 1 cut(s) 424
Cfr10I RCCGGY 1 cut(s) 246
Cfr13I GGNCC 4 cut(s) 277, 289, 342, 429
Cfr9I CCCGGG 2 cut(s) 260, 431
CseI GACGC 1 cut(s) 537
Csp6I GTAC 1 cut(s) 475
CspAI ACCGGT 1 cut(s) 246
CspCI CAANNNNNGTGG 2 cut(s) 312, 347
CviAII CATG 3 cut(s) 144, 286, 553
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 2 cut(s) 117, 425
DpnI GATC 2 cut(s) 78, 105
DpnII GATC 2 cut(s) 76, 103
EaeI YGGCCR 2 cut(s) 55, 510
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 175
EarI CTCTTC 1 cut(s) 175
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 4 cut(s) 285, 338, 453, 552
Eco24I GRGCYC 2 cut(s) 267, 519
Eco52I CGGCCG 1 cut(s) 55
Eco81I CCTNAGG 1 cut(s) 117
Eco88I CYCGRG 3 cut(s) 260, 291, 431
EcoO109I RGGNCCY 1 cut(s) 342
EcoRII CCWGG 4 cut(s) 84, 202, 532, 632
EcoT14I CCWWGG 4 cut(s) 285, 338, 453, 552
EcoT22I ATGCAT 1 cut(s) 149
EcoT38I GRGCYC 2 cut(s) 267, 519
ErhI CCWWGG 4 cut(s) 285, 338, 453, 552
FaeI CATG 3 cut(s) 147, 289, 556
FaiI YATR 5 cut(s) 98, 145, 287, 302, 554
FatI CATG 3 cut(s) 143, 285, 552
Fnu4HI GCNGC 2 cut(s) 58, 630
FokI GGATG 2 cut(s) 321, 553
FriOI GRGCYC 2 cut(s) 267, 519
Fsp4HI GCNGC 2 cut(s) 58, 630
FspBI CTAG 2 cut(s) 44, 161
GluI GCNGC 2 cut(s) 58, 630
GsaI CCCAGC 1 cut(s) 421
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 7 cut(s) 57, 279, 290, 344, 430, 512, 557
HapII CCGG 7 cut(s) 74, 107, 247, 261, 406, 432, 513
HgaI GACGC 1 cut(s) 537
Hin1II CATG 3 cut(s) 147, 289, 556
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HinfI GANTC 3 cut(s) 437, 485, 637
HpaII CCGG 7 cut(s) 74, 107, 247, 261, 406, 432, 513
HphI GGTGA 2 cut(s) 172, 232
Hpy166II GTNNAC 5 cut(s) 140, 298, 394, 539, 564
Hpy188I TCNGA 6 cut(s) 70, 349, 571, 612, 642, 653
Hpy188III TCNNGA 3 cut(s) 107, 119, 589
Hpy8I GTNNAC 5 cut(s) 140, 298, 394, 539, 564
Hpy99I CGWCG 1 cut(s) 590
HpyAV CCTTC 2 cut(s) 386, 585
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 5 cut(s) 96, 140, 147, 334, 539
HpyF10VI GCNNNNNNNGC 4 cut(s) 369, 427, 545, 554
HpyF3I CTNAG 2 cut(s) 117, 425
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 3 cut(s) 147, 289, 556
Kpn2I TCCGGA 1 cut(s) 106
Kzo9I GATC 2 cut(s) 76, 103
LmnI GCTCC 1 cut(s) 87
Lsp1109I GCAGC 1 cut(s) 616
LweI GCATC 2 cut(s) 295, 343
MaeI CTAG 2 cut(s) 44, 161
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 3 cut(s) 232, 352, 617
MalI GATC 2 cut(s) 78, 105
MbiI CCGCTC 1 cut(s) 82
MboI GATC 2 cut(s) 76, 103
MboII GAAGA 2 cut(s) 162, 610
MflI RGATCY 2 cut(s) 76, 103
MhlI GDGCHC 4 cut(s) 142, 267, 519, 541
MluCI AATT 3 cut(s) 13, 29, 122
MlyI GAGTC 2 cut(s) 446, 479
MmeI TCCRAC 2 cut(s) 93, 471
Mph1103I ATGCAT 1 cut(s) 149
MroI TCCGGA 1 cut(s) 106
MspI CCGG 7 cut(s) 74, 107, 247, 261, 406, 432, 513
MvaI CCWGG 4 cut(s) 86, 204, 534, 634
MwoI GCNNNNNNNGC 4 cut(s) 369, 427, 545, 554
NciI CCSGG 7 cut(s) 74, 261, 262, 407, 432, 433, 514
NcoI CCATGG 2 cut(s) 285, 552
NdeII GATC 2 cut(s) 76, 103
NlaIII CATG 3 cut(s) 147, 289, 556
NlaIV GGNNCC 4 cut(s) 78, 105, 495, 647
NmuCI GTSAC 3 cut(s) 232, 352, 617
NsiI ATGCAT 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PfeI GAWTC 1 cut(s) 637
PfoI TCCNGGA 1 cut(s) 84
PinAI ACCGGT 1 cut(s) 246
PkrI GCNGC 2 cut(s) 59, 631
PleI GAGTC 2 cut(s) 445, 479
PpsI GAGTC 2 cut(s) 445, 479
Psp6I CCWGG 4 cut(s) 84, 202, 532, 632
PspFI CCCAGC 1 cut(s) 417
PspGI CCWGG 4 cut(s) 84, 202, 532, 632
PspN4I GGNNCC 4 cut(s) 78, 105, 495, 647
PspPI GGNCC 4 cut(s) 277, 289, 342, 429
PstNI CAGNNNCTG 1 cut(s) 424
PsuI RGATCY 2 cut(s) 76, 103
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
SatI GCNGC 2 cut(s) 58, 630
Sau3AI GATC 2 cut(s) 76, 103
Sau96I GGNCC 4 cut(s) 277, 289, 342, 429
SchI GAGTC 2 cut(s) 446, 479
SduI GDGCHC 4 cut(s) 142, 267, 519, 541
SfaNI GCATC 2 cut(s) 295, 343
SmaI CCCGGG 2 cut(s) 262, 433
SrfI GCCCGGGC 1 cut(s) 262
Sse9I AATT 3 cut(s) 13, 29, 122
SsiI CCGC 3 cut(s) 58, 80, 101
SspMI CTAG 2 cut(s) 44, 161
StyI CCWWGG 4 cut(s) 285, 338, 453, 552
TaiI ACGT 1 cut(s) 234
TaqI TCGA 1 cut(s) 588
TasI AATT 3 cut(s) 13, 29, 122
TatI WGTACW 1 cut(s) 474
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 1 cut(s) 637
TseFI GTSAC 3 cut(s) 232, 352, 617
TseI GCWGC 1 cut(s) 629
Tsp45I GTSAC 3 cut(s) 232, 352, 617
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 2 cut(s) 260, 431
VneI GTGCAC 2 cut(s) 138, 537
XapI RAATTY 1 cut(s) 122
XmaI CCCGGG 2 cut(s) 260, 431
XspI CTAG 2 cut(s) 44, 161
Zsp2I ATGCAT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.