MD16G1084100.v1.1

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016191
Physical Location & Seq
Reverse (-)
5910930 .. 5911589
660 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1084100.v1.1.491

Sequence Viewer

Length: 660 bp
ATGAACACAATCAATTATCTATTTCTCCAATTATTCCTCAACCTAGCTCTATTCGGCCGGGCCAAATCCGACCCGGATCCGCTCCAGGACTACTGCGTAGCGGACCCGGAAACCCCTCAGACATTTTCCCTCAATGGTGCGCCATGCATTGACCCAAACCTAGCAATCTCTTCCCACTTCACCACATCAGTCCTATCCAAACCAGGCGACACTAAAGCCAACGGGTTCAACGTGACCCTCACCAACCCGTTCAATCTGCCCGGGCTCAACACATTGGGCCTGACCATGGCCCGAATTGACATAGATGCCAATGGGATTGTGCCACCTCACTTGCATCCAAGGGCCTCGGAGGTCACAATCTGCCTCACGGGCTTCCTTCTTGTGGGCTTTGTGGACACATCAAACCGGGTCTTTACCCAGCAATTGAGGCCCGGTGAGTCTTTTGTGTTCCCCAAGGGTTTGGTACATTTTCTGTACAACTACGACTCGGAGGAACCAGCTGTGGCATTGGCCGGGCTCGGGAGCCAAAACCCTGGTGCACAGATTGTGTCTATGGCCTCGTTCACATCCGAGCCTTCAAACCCCGTCAAGATTTTGAAGAAAGGGTTTCAGATTAGTGACCTAAACGCTGCCTGGATTCGAAGGAACCTTGGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

220

Amino Acids

23.67

Weight (kDa)

5.74

Isoelectric Point (pI)

28.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 66 - 207 2.2e-39 Cupin
Cupin_2 PF07883 100 - 170 1.7e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10460
fragaria_vesca FvH4_4g35250
malus_domestica MD16G1084100.v1.1
prunus_persica Prupe.1G261900_v2.0.a1
pyrus_communis pycom16g07310
rosa_chinensis RchiOBHm_Chr4g0444861
rosa_laevigata RLG00000005794
rosa_multiflora Rmu_sc0006845.1_g000007
rosa_roxburghii Rroxscaffold_5G00385350
rosa_rugosa Rorug04G0355700
rosa_samantha Rh4AG417200 Rh4BG429000 Rh4CG443700 Rh4DG424900
rosa_wichuraiana Rw4G035760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 82
AciI CCGC 2 cut(s) 80, 101
AclWI GGATC 2 cut(s) 71, 84
AcoI YGGCCR 2 cut(s) 55, 510
AfaI GTAC 2 cut(s) 465, 476
AfiI CCNNNNNNNGG 3 cut(s) 286, 382, 519
AgsI TTSAA 4 cut(s) 229, 253, 579, 598
AjnI CCWGG 4 cut(s) 84, 202, 532, 632
AluBI AGCT 2 cut(s) 47, 500
AluI AGCT 2 cut(s) 47, 500
Alw21I GWGCWC 1 cut(s) 541
Alw44I GTGCAC 1 cut(s) 537
AlwI GGATC 2 cut(s) 71, 84
Ama87I CYCGRG 2 cut(s) 260, 518
AoxI GGCC 8 cut(s) 55, 60, 277, 288, 342, 428, 510, 555
ApaLI GTGCAC 1 cut(s) 537
ApeKI GCWGC 1 cut(s) 629
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 6 cut(s) 60, 103, 277, 289, 342, 429
AsuC2I CCSGG 8 cut(s) 59, 74, 107, 261, 262, 407, 432, 514
AsuHPI GGTGA 3 cut(s) 172, 232, 446
AsuII TTCGAA 1 cut(s) 640
AvaI CYCGRG 2 cut(s) 260, 518
AvaII GGWCC 1 cut(s) 103
BaeGI GKGCMC 1 cut(s) 541
BamHI GGATCC 1 cut(s) 76
BanII GRGCYC 2 cut(s) 267, 519
Bbv12I GWGCWC 1 cut(s) 541
BbvI GCAGC 1 cut(s) 616
BciT130I CCWGG 4 cut(s) 86, 204, 534, 634
BcnI CCSGG 8 cut(s) 59, 74, 107, 261, 262, 407, 432, 514
BfaI CTAG 2 cut(s) 44, 161
BglI GCCNNNNNGGC 1 cut(s) 369
BisI GCNGC 1 cut(s) 630
BlsI GCNGC 1 cut(s) 631
Bme18I GGWCC 1 cut(s) 103
BmeT110I CYCGRG 2 cut(s) 260, 518
BmgT120I GGNCC 6 cut(s) 60, 103, 277, 289, 342, 429
BmiI GGNNCC 5 cut(s) 78, 105, 495, 524, 647
BmsI GCATC 2 cut(s) 295, 343
BpmI CTGGAG 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 640
BpuMI CCSGG 8 cut(s) 59, 74, 107, 261, 262, 407, 432, 514
BsaJI CCNNGG 7 cut(s) 260, 285, 338, 345, 453, 532, 649
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 382, 519
BseBI CCWGG 4 cut(s) 86, 204, 534, 634
BseDI CCNNGG 7 cut(s) 260, 285, 338, 345, 453, 532, 649
BseGI GGATG 2 cut(s) 334, 566
BseLI CCNNNNNNNGG 3 cut(s) 286, 382, 519
BseMII CTCAG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 541
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 1 cut(s) 616
BseYI CCCAGC 1 cut(s) 417
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 8 cut(s) 57, 62, 279, 290, 344, 430, 512, 557
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 541
BsiHKCI CYCGRG 2 cut(s) 260, 518
BsiSI CCGG 7 cut(s) 58, 74, 107, 261, 406, 432, 513
BslI CCNNNNNNNGG 3 cut(s) 286, 382, 519
BsnI GGCC 8 cut(s) 57, 62, 279, 290, 344, 430, 512, 557
BsoBI CYCGRG 2 cut(s) 260, 518
Bsp119I TTCGAA 1 cut(s) 640
Bsp1286I GDGCHC 3 cut(s) 267, 519, 541
Bsp1407I TGTACA 1 cut(s) 474
Bsp143I GATC 1 cut(s) 76
Bsp19I CCATGG 1 cut(s) 285
BspACI CCGC 2 cut(s) 80, 101
BspANI GGCC 8 cut(s) 57, 62, 279, 290, 344, 430, 512, 557
BspCNI CTCAG 1 cut(s) 130
BspLI GGNNCC 5 cut(s) 78, 105, 495, 524, 647
BspPI GGATC 2 cut(s) 71, 84
BspT104I TTCGAA 1 cut(s) 640
BsrBI CCGCTC 1 cut(s) 82
BsrGI TGTACA 1 cut(s) 474
BssECI CCNNGG 7 cut(s) 260, 285, 338, 345, 453, 532, 649
BssMI GATC 1 cut(s) 76
BssT1I CCWWGG 4 cut(s) 285, 338, 453, 649
Bst2UI CCWGG 4 cut(s) 86, 204, 534, 634
Bst6I CTCTTC 1 cut(s) 175
BstAUI TGTACA 1 cut(s) 474
BstBI TTCGAA 1 cut(s) 640
BstDEI CTNAG 1 cut(s) 117
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 334, 566
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 1 cut(s) 79
BstMBI GATC 1 cut(s) 76
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 2 cut(s) 369, 427
BstNI CCWGG 4 cut(s) 86, 204, 534, 634
BstSLI GKGCMC 1 cut(s) 541
BstV1I GCAGC 1 cut(s) 616
BstX2I RGATCY 1 cut(s) 76
BstXI CCANNNNNNTGG 2 cut(s) 460, 533
BstYI RGATCY 1 cut(s) 76
BstZI CGGCCG 1 cut(s) 55
BsuRI GGCC 8 cut(s) 57, 62, 279, 290, 344, 430, 512, 557
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 2 cut(s) 334, 566
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 6 cut(s) 60, 103, 277, 289, 342, 429
Cfr9I CCCGGG 1 cut(s) 260
Csp6I GTAC 2 cut(s) 464, 475
CspCI CAANNNNNGTGG 2 cut(s) 312, 347
CviAII CATG 2 cut(s) 144, 286
CviQI GTAC 2 cut(s) 464, 475
DdeI CTNAG 1 cut(s) 117
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
EaeI YGGCCR 2 cut(s) 55, 510
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 175
EarI CTCTTC 1 cut(s) 175
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 4 cut(s) 285, 338, 453, 649
Eco24I GRGCYC 2 cut(s) 267, 519
Eco47I GGWCC 1 cut(s) 103
Eco52I CGGCCG 1 cut(s) 55
Eco88I CYCGRG 2 cut(s) 260, 518
EcoO109I RGGNCCY 1 cut(s) 342
EcoRII CCWGG 4 cut(s) 84, 202, 532, 632
EcoT14I CCWWGG 4 cut(s) 285, 338, 453, 649
EcoT22I ATGCAT 1 cut(s) 149
EcoT38I GRGCYC 2 cut(s) 267, 519
ErhI CCWWGG 4 cut(s) 285, 338, 453, 649
FaeI CATG 2 cut(s) 147, 289
FaiI YATR 4 cut(s) 145, 287, 302, 554
FatI CATG 2 cut(s) 143, 285
Fnu4HI GCNGC 1 cut(s) 630
FokI GGATG 2 cut(s) 321, 553
FriOI GRGCYC 2 cut(s) 267, 519
Fsp4HI GCNGC 1 cut(s) 630
FspBI CTAG 2 cut(s) 44, 161
GlaI GCGC 1 cut(s) 141
GluI GCNGC 1 cut(s) 630
GsaI CCCAGC 1 cut(s) 421
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 8 cut(s) 57, 62, 279, 290, 344, 430, 512, 557
HapII CCGG 7 cut(s) 58, 74, 107, 261, 406, 432, 513
HhaI GCGC 1 cut(s) 142
Hin1II CATG 2 cut(s) 147, 289
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HinfI GANTC 3 cut(s) 437, 485, 637
HpaII CCGG 7 cut(s) 58, 74, 107, 261, 406, 432, 513
HphI GGTGA 3 cut(s) 172, 232, 446
Hpy166II GTNNAC 3 cut(s) 394, 539, 564
Hpy188I TCNGA 6 cut(s) 70, 120, 349, 490, 571, 612
Hpy188III TCNNGA 2 cut(s) 520, 589
Hpy8I GTNNAC 3 cut(s) 394, 539, 564
HpyAV CCTTC 3 cut(s) 386, 585, 636
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 147, 334, 539
HpyF10VI GCNNNNNNNGC 2 cut(s) 369, 427
HpyF3I CTNAG 1 cut(s) 117
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 2 cut(s) 147, 289
HspAI GCGC 1 cut(s) 140
Kzo9I GATC 1 cut(s) 76
LmnI GCTCC 2 cut(s) 87, 522
Lsp1109I GCAGC 1 cut(s) 616
LweI GCATC 2 cut(s) 295, 343
MaeI CTAG 2 cut(s) 44, 161
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 3 cut(s) 232, 352, 617
MalI GATC 1 cut(s) 78
MbiI CCGCTC 1 cut(s) 82
MboI GATC 1 cut(s) 76
MboII GAAGA 2 cut(s) 162, 610
MfeI CAATTG 1 cut(s) 422
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 3 cut(s) 267, 519, 541
MluCI AATT 4 cut(s) 13, 29, 294, 422
MlyI GAGTC 2 cut(s) 446, 479
MmeI TCCRAC 1 cut(s) 93
Mph1103I ATGCAT 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 500
MspI CCGG 7 cut(s) 58, 74, 107, 261, 406, 432, 513
MunI CAATTG 1 cut(s) 422
MvaI CCWGG 4 cut(s) 86, 204, 534, 634
MwoI GCNNNNNNNGC 2 cut(s) 369, 427
NciI CCSGG 8 cut(s) 59, 74, 107, 261, 262, 407, 432, 514
NcoI CCATGG 1 cut(s) 285
NdeII GATC 1 cut(s) 76
NlaIII CATG 2 cut(s) 147, 289
NlaIV GGNNCC 5 cut(s) 78, 105, 495, 524, 647
NmuCI GTSAC 3 cut(s) 232, 352, 617
NsiI ATGCAT 1 cut(s) 149
NspV TTCGAA 1 cut(s) 640
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PfeI GAWTC 1 cut(s) 637
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 1 cut(s) 631
PleI GAGTC 2 cut(s) 445, 479
PpsI GAGTC 2 cut(s) 445, 479
Psp6I CCWGG 4 cut(s) 84, 202, 532, 632
PspFI CCCAGC 1 cut(s) 417
PspGI CCWGG 4 cut(s) 84, 202, 532, 632
PspN4I GGNNCC 5 cut(s) 78, 105, 495, 524, 647
PspPI GGNCC 6 cut(s) 60, 103, 277, 289, 342, 429
PsuI RGATCY 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 2 cut(s) 465, 476
RsaNI GTAC 2 cut(s) 464, 475
SatI GCNGC 1 cut(s) 630
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 6 cut(s) 60, 103, 277, 289, 342, 429
SchI GAGTC 2 cut(s) 446, 479
SduI GDGCHC 3 cut(s) 267, 519, 541
SfaNI GCATC 2 cut(s) 295, 343
SfuI TTCGAA 1 cut(s) 640
SinI GGWCC 1 cut(s) 103
SmaI CCCGGG 1 cut(s) 262
SrfI GCCCGGGC 1 cut(s) 262
Sse9I AATT 4 cut(s) 13, 29, 294, 422
SsiI CCGC 2 cut(s) 80, 101
SspMI CTAG 2 cut(s) 44, 161
StyI CCWWGG 4 cut(s) 285, 338, 453, 649
TaiI ACGT 1 cut(s) 234
TaqI TCGA 1 cut(s) 640
TasI AATT 4 cut(s) 13, 29, 294, 422
TatI WGTACW 1 cut(s) 474
TfiI GAWTC 1 cut(s) 637
TseFI GTSAC 3 cut(s) 232, 352, 617
TseI GCWGC 1 cut(s) 629
Tsp45I GTSAC 3 cut(s) 232, 352, 617
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 260
VneI GTGCAC 1 cut(s) 537
VpaK11BI GGWCC 1 cut(s) 103
XmaI CCCGGG 1 cut(s) 260
XspI CTAG 2 cut(s) 44, 161
Zsp2I ATGCAT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.