FvH4_4g35530

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012438
Physical Location & Seq
Reverse (-)
32808420 .. 32810345
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35530.t1

Sequence Viewer

Length: 909 bp
ATGAAGCTTAGTCATCCTTCAACTACTGTGATCACCTCCTTCATCTTCACCTTCCTTACCCTACCCTCCTCTATCACATCCCAAATCTGCCAAAGATCCTGCGACAAAATCCCTCTCAGATTCCCGTTCGGCAGTGACGCCGGCTGCGGCGACCCGCGCTTCCACCAATCCGTGACATGCAACCAAGACAAGCTCACCTTCACCACCCACACCGGATCCTACCCCATCACCAACATAGACTACACAAAACAAGTCCTCTACATCTCCGACCCTTCCATGTCCACCTGCTCTTGCACTCAACCCAGCAAAGGCTTCGGCCTCAACTACGACGCGCCGTTCACCTTCCAAGACGACAGCATCTTCGCCTTGCTCGACTGCTCCACGTCGTCATCATCCAACGGCAGCCTATACAGCAACGACAACTCCAAGCTCTCTCTTTGTGACAACCAAGGTTCACCCATCTGCAGCTTCTTGTATTCATGCAGGGCAATAAGTTACATCAATCTCCCAATCTCCACGTGTTGCGTTTACACGCCGGTGGATCTCGGGCCGGCTTTCGAGATGGACTTGCAGAAGCTACAATGCAGCTCTTACTCTGGTTTTTACAGCTTCAATGGCCGTGAATCCGACCCTAATAGTTGGAAGTATGGGATAGCGCTCAAGTATAAGTTCAACGTGAACAATGAGTATCCGAGTTCGTGTTCTAGTTGTGAGAGGAGTAATGGGGTTTGTGGGTACTATGGAGTTTATAACTCGTTCATCTGTAACTGTCCGAATGGTTTGAACACTACAAACGATTGCTACTTTGGATCGTCCTATAGTTATGGCTCGAGGCTTATTCCATCTTGGCACTCTGCAGGGATCTGGCTCTATTGTTTGGCATGGCTATTGGTTTCAGTACTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

303

Amino Acids

33.41

Weight (kDa)

5.74

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 30 - 90 8.9e-11 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 208 - 259 4.2e-08 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10380 AT1G10380
fragaria_vesca FvH4_4g35530
malus_domestica MD13G1082400.v1.1 MD16G1081500.v1.1
prunus_persica Prupe.3G121800_v2.0.a1 Prupe.3G121800_v2.0.a1
pyrus_communis pycom13g07100 pycom16g07030
rosa_chinensis RchiOBHm_Chr4g0445181
rosa_laevigata RLG00000005760
rosa_multiflora Rmu_sc0000050.1_g000037
rosa_roxburghii Rroxscaffold_5G00385690
rosa_rugosa Rorug04G0359700
rosa_samantha Rh4AG420500 Rh4BG432400 Rh4CG447000 Rh4DG428500
rosa_wichuraiana Rw4G036060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AarI CACCTGC 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 293
AccII CGCG 2 cut(s) 157, 332
AciI CCGC 2 cut(s) 147, 155
AclWI GGATC 6 cut(s) 90, 210, 223, 549, 817, 869
AcoI YGGCCR 1 cut(s) 616
AcvI CACGTG 1 cut(s) 519
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 2 cut(s) 737, 900
AfeI AGCGCT 1 cut(s) 657
AfiI CCNNNNNNNGG 1 cut(s) 308
AflIII ACRYGT 1 cut(s) 518
AgsI TTSAA 4 cut(s) 21, 613, 673, 784
AjiI CACGTC 1 cut(s) 384
AleI CACNNNNGTG 1 cut(s) 536
AluBI AGCT 7 cut(s) 7, 193, 430, 468, 577, 588, 609
AluI AGCT 7 cut(s) 7, 193, 430, 468, 577, 588, 609
AlwI GGATC 6 cut(s) 90, 210, 223, 549, 817, 869
Ama87I CYCGRG 2 cut(s) 545, 829
Aor51HI AGCGCT 1 cut(s) 657
AoxI GGCC 3 cut(s) 316, 548, 616
ApeKI GCWGC 4 cut(s) 144, 402, 465, 585
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AspLEI GCGC 3 cut(s) 159, 334, 658
AspS9I GGNCC 1 cut(s) 548
AsuHPI GGTGA 7 cut(s) 25, 40, 187, 193, 220, 331, 447
AvaI CYCGRG 2 cut(s) 545, 829
BamHI GGATCC 1 cut(s) 215
BbrPI CACGTG 1 cut(s) 519
BbvI GCAGC 4 cut(s) 131, 414, 477, 597
BccI CCATC 4 cut(s) 233, 467, 556, 850
BceAI ACGGC 3 cut(s) 319, 415, 603
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciVI GTATCC 1 cut(s) 699
BclI TGATCA 1 cut(s) 30
BfaI CTAG 1 cut(s) 705
BfmI CTRYAG 3 cut(s) 463, 817, 855
BfoI RGCGCY 1 cut(s) 659
BfuAI ACCTGC 1 cut(s) 293
BfuI GTATCC 1 cut(s) 699
BisI GCNGC 5 cut(s) 145, 148, 403, 466, 586
BlsI GCNGC 5 cut(s) 146, 149, 404, 467, 587
BmcAI AGTACT 1 cut(s) 900
BmeT110I CYCGRG 2 cut(s) 545, 829
BmgBI CACGTC 1 cut(s) 384
BmgT120I GGNCC 1 cut(s) 548
BmiI GGNNCC 1 cut(s) 217
BmsI GCATC 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 644
BsaAI YACGTR 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 448
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 407, 437
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse118I RCCGGY 3 cut(s) 140, 535, 550
BseDI CCNNGG 1 cut(s) 448
BseGI GGATG 3 cut(s) 13, 77, 392
BseLI CCNNNNNNNGG 1 cut(s) 308
BseMII CTCAG 1 cut(s) 130
BseRI GAGGAG 2 cut(s) 58, 730
BseXI GCAGC 4 cut(s) 131, 414, 477, 597
BseYI CCCAGC 1 cut(s) 302
Bsh1236I CGCG 2 cut(s) 157, 332
BshFI GGCC 3 cut(s) 318, 550, 618
BsiHKCI CYCGRG 2 cut(s) 545, 829
BsiSI CCGG 4 cut(s) 141, 213, 536, 551
BslI CCNNNNNNNGG 1 cut(s) 308
BsnI GGCC 3 cut(s) 318, 550, 618
BsoBI CYCGRG 2 cut(s) 545, 829
Bsp143I GATC 6 cut(s) 30, 95, 215, 541, 809, 861
BspACI CCGC 2 cut(s) 147, 155
BspANI GGCC 3 cut(s) 318, 550, 618
BspCNI CTCAG 1 cut(s) 129
BspFNI CGCG 2 cut(s) 157, 332
BspLI GGNNCC 1 cut(s) 217
BspMAI CTGCAG 2 cut(s) 467, 859
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 6 cut(s) 90, 210, 223, 549, 817, 869
BsrFI RCCGGY 3 cut(s) 140, 535, 550
BssAI RCCGGY 3 cut(s) 140, 535, 550
BssECI CCNNGG 1 cut(s) 448
BssMI GATC 6 cut(s) 30, 95, 215, 541, 809, 861
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 448
Bst4CI ACNGT 2 cut(s) 28, 770
BstACI GRCGYC 1 cut(s) 138
BstBAI YACGTR 1 cut(s) 519
BstC8I GCNNGC 2 cut(s) 142, 552
BstDEI CTNAG 2 cut(s) 8, 116
BstF5I GGATG 3 cut(s) 13, 77, 392
BstFNI CGCG 2 cut(s) 157, 332
BstH2I RGCGCY 1 cut(s) 659
BstHHI GCGC 3 cut(s) 159, 334, 658
BstKTI GATC 6 cut(s) 33, 98, 218, 544, 812, 864
BstMBI GATC 6 cut(s) 30, 95, 215, 541, 809, 861
BstMWI GCNNNNNNNGC 3 cut(s) 156, 411, 615
BstNSI RCATGY 1 cut(s) 180
BstSFI CTRYAG 3 cut(s) 463, 817, 855
BstUI CGCG 2 cut(s) 157, 332
BstV1I GCAGC 4 cut(s) 131, 414, 477, 597
BstX2I RGATCY 4 cut(s) 95, 215, 541, 861
BstYI RGATCY 4 cut(s) 95, 215, 541, 861
BsuI GTATCC 1 cut(s) 699
BsuRI GGCC 3 cut(s) 318, 550, 618
BtrI CACGTC 1 cut(s) 384
BtsCI GGATG 3 cut(s) 13, 77, 392
BtsI GCAGTG 1 cut(s) 139
BtsIMutI CAGTG 1 cut(s) 139
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 2 cut(s) 142, 552
CfoI GCGC 3 cut(s) 159, 334, 658
Cfr10I RCCGGY 3 cut(s) 140, 535, 550
Cfr13I GGNCC 1 cut(s) 548
CseI GACGC 2 cut(s) 146, 338
Csp6I GTAC 2 cut(s) 736, 899
CviAII CATG 4 cut(s) 177, 277, 480, 882
CviQI GTAC 2 cut(s) 736, 899
DdeI CTNAG 2 cut(s) 8, 116
DpnI GATC 6 cut(s) 32, 97, 217, 543, 811, 863
DpnII GATC 6 cut(s) 30, 95, 215, 541, 809, 861
EaeI YGGCCR 1 cut(s) 616
Eco130I CCWWGG 1 cut(s) 448
Eco47III AGCGCT 1 cut(s) 657
Eco72I CACGTG 1 cut(s) 519
Eco88I CYCGRG 2 cut(s) 545, 829
EcoT14I CCWWGG 1 cut(s) 448
ErhI CCWWGG 1 cut(s) 448
FaeI CATG 4 cut(s) 180, 280, 483, 885
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FatI CATG 4 cut(s) 176, 276, 479, 881
FauI CCCGC 1 cut(s) 162
FbaI TGATCA 1 cut(s) 30
Fnu4HI GCNGC 5 cut(s) 145, 148, 403, 466, 586
FokI GGATG 2 cut(s) 64, 379
Fsp4HI GCNGC 5 cut(s) 145, 148, 403, 466, 586
FspBI CTAG 1 cut(s) 705
GlaI GCGC 3 cut(s) 158, 333, 657
GluI GCNGC 5 cut(s) 145, 148, 403, 466, 586
GsaI CCCAGC 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 659
HaeIII GGCC 3 cut(s) 318, 550, 618
HapII CCGG 4 cut(s) 141, 213, 536, 551
HgaI GACGC 2 cut(s) 146, 338
HhaI GCGC 3 cut(s) 159, 334, 658
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 4 cut(s) 180, 280, 483, 885
Hin6I GCGC 3 cut(s) 157, 332, 656
HinP1I GCGC 3 cut(s) 157, 332, 656
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 2 cut(s) 120, 623
HpaII CCGG 4 cut(s) 141, 213, 536, 551
HphI GGTGA 7 cut(s) 25, 40, 187, 193, 220, 331, 447
Hpy166II GTNNAC 5 cut(s) 282, 339, 455, 529, 679
Hpy188I TCNGA 5 cut(s) 119, 268, 628, 693, 774
Hpy188III TCNNGA 1 cut(s) 559
Hpy8I GTNNAC 5 cut(s) 282, 339, 455, 529, 679
Hpy99I CGWCG 2 cut(s) 332, 388
HpyAV CCTTC 6 cut(s) 27, 49, 61, 208, 282, 352
HpyCH4III ACNGT 2 cut(s) 28, 770
HpyCH4IV ACGT 3 cut(s) 383, 518, 675
HpyCH4V TGCA 7 cut(s) 180, 294, 465, 483, 571, 585, 857
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 411, 615
HpyF3I CTNAG 2 cut(s) 8, 116
HpySE526I ACGT 3 cut(s) 383, 518, 675
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 4 cut(s) 180, 280, 483, 885
HspAI GCGC 3 cut(s) 157, 332, 656
KroI GCCGGC 2 cut(s) 140, 550
KroNI GCCGGC 2 cut(s) 142, 552
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 6 cut(s) 30, 95, 215, 541, 809, 861
LmnI GCTCC 1 cut(s) 383
Lsp1109I GCAGC 4 cut(s) 131, 414, 477, 597
LweI GCATC 1 cut(s) 366
MaeI CTAG 1 cut(s) 705
MaeII ACGT 3 cut(s) 383, 518, 675
MaeIII GTNAC 5 cut(s) 134, 172, 440, 494, 764
MalI GATC 6 cut(s) 32, 97, 217, 543, 811, 863
MboI GATC 6 cut(s) 30, 95, 215, 541, 809, 861
MboII GAAGA 2 cut(s) 37, 352
MflI RGATCY 4 cut(s) 95, 215, 541, 861
MmeI TCCRAC 4 cut(s) 291, 420, 620, 651
MnlI CCTC 8 cut(s) 46, 76, 79, 123, 266, 329, 708, 825
MroNI GCCGGC 2 cut(s) 140, 550
MslI CAYNNNNRTG 1 cut(s) 536
MspI CCGG 4 cut(s) 141, 213, 536, 551
MvnI CGCG 2 cut(s) 157, 332
MwoI GCNNNNNNNGC 3 cut(s) 156, 411, 615
NaeI GCCGGC 2 cut(s) 142, 552
NdeII GATC 6 cut(s) 30, 95, 215, 541, 809, 861
NgoMIV GCCGGC 2 cut(s) 140, 550
NlaIII CATG 4 cut(s) 180, 280, 483, 885
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 3 cut(s) 134, 172, 440
NspI RCATGY 1 cut(s) 180
OliI CACNNNNGTG 1 cut(s) 536
PaeR7I CTCGAG 1 cut(s) 829
PaqCI CACCTGC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PdiI GCCGGC 2 cut(s) 142, 552
PfeI GAWTC 2 cut(s) 120, 623
PkrI GCNGC 5 cut(s) 146, 149, 404, 467, 587
PmaCI CACGTG 1 cut(s) 519
PmlI CACGTG 1 cut(s) 519
Ppu21I YACGTR 1 cut(s) 519
PsiI TTATAA 1 cut(s) 750
PspCI CACGTG 1 cut(s) 519
PspFI CCCAGC 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 548
PspXI VCTCGAGB 1 cut(s) 829
PstI CTGCAG 2 cut(s) 467, 859
PsuI RGATCY 4 cut(s) 95, 215, 541, 861
RsaI GTAC 2 cut(s) 737, 900
RsaNI GTAC 2 cut(s) 736, 899
RseI CAYNNNNRTG 1 cut(s) 536
SatI GCNGC 5 cut(s) 145, 148, 403, 466, 586
Sau3AI GATC 6 cut(s) 30, 95, 215, 541, 809, 861
Sau96I GGNCC 1 cut(s) 548
ScaI AGTACT 1 cut(s) 900
SfaNI GCATC 1 cut(s) 366
SfcI CTRYAG 3 cut(s) 463, 817, 855
Sfr274I CTCGAG 1 cut(s) 829
SgrAI CRCCGGYG 1 cut(s) 535
SlaI CTCGAG 1 cut(s) 829
SmiMI CAYNNNNRTG 1 cut(s) 536
SmlI CTYRAG 2 cut(s) 659, 829
SmoI CTYRAG 2 cut(s) 659, 829
SsiI CCGC 2 cut(s) 147, 155
SspMI CTAG 1 cut(s) 705
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 2 cut(s) 28, 770
TaiI ACGT 3 cut(s) 386, 521, 678
TaqI TCGA 3 cut(s) 372, 558, 830
TatI WGTACW 1 cut(s) 898
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 2 cut(s) 120, 623
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 3 cut(s) 134, 172, 440
TseI GCWGC 4 cut(s) 144, 402, 465, 585
Tsp45I GTSAC 3 cut(s) 134, 172, 440
TspDTI ATGAA 4 cut(s) 17, 31, 468, 748
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 1 cut(s) 139
XceI RCATGY 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 829
XspI CTAG 1 cut(s) 705
ZrmI AGTACT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.