Rw4G036060

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012438
Physical Location & Seq
Reverse (-)
62870012 .. 62872133
2122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G036060.1

Sequence Viewer

Length: 906 bp
ATGAAGCTTAGTCATCCTTCAACTACTATAACCTCCTTCATCTTCTTCATCTTCCTTACTCTACCCTCCTCAATCACATCCCAAATTTGCCAAAGATCCTGCAACAGAATCCCTCTTAACTTCCCATTCGGCAGTGACGCCGGCTGCGGCGACCCGCGCTTCCACCAATCCGTAACATGCAACCAAGACAGGCTCACCTTCACCACCCACACCGGATCCTACCCTATCACCAACATAGACTACACAAAACAAGTCCTCTACATCTCAGACCCCTCCATGTCCACCTGCTCCTGCACTCAACCCAGCAAAGGCTTCAGCCTCAACTACGACGCTCCGTTCACCTTCCAAGACGACACCATCTTCGCCTTGCTCGACTGCTCCACCTCATCATCCAACGGCAGCCTTTACAGCAGTGACAACTCCAAGCTCTCTCTTTGTGATAACCAAGGTGCACCCATCTGCAGCTACTTGTATTCATGCAGGGCTATAAGCTTCATCAATCTTCCCATCGCCACGTGTTGCGTTTACACGCCGGTGGATCTCGGGCCCGCTTTCGAGATGGACTTGCAGAAGCTACAATGCAGCTCTTATTCTGGTTTTTACAGCTTCAATGGCCGTGAATCCGACCCTAATAGTTGGAAGTATGGGATAGCGCTCAAGTACAAGTTCAATGTGAATAATGAGTTTCCCATTTCATGTTTTAATTGTGAGAGGAGTAATGGGGTTTGTGGGTACTATGGAGCTTATAACTCGTTTATATGTAATTGTCCGAATGGTTTGAACACTACAAACGATTGCTACTTTGGATCGCCCTATAGTTATGGCTCGAGGCTTATTGCATCGTGGCACTCTGGGACCTGGCTATTCTATTGTTTGGTGTGGCTATTGGTTTCAGTACACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

301

Amino Acids

33.55

Weight (kDa)

5.9

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 30 - 90 7.3e-10 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 207 - 258 1.2e-06 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10380 AT1G10380
fragaria_vesca FvH4_4g35530
malus_domestica MD13G1082400.v1.1 MD16G1081500.v1.1
prunus_persica Prupe.3G121800_v2.0.a1 Prupe.3G121800_v2.0.a1
pyrus_communis pycom13g07100 pycom16g07030
rosa_chinensis RchiOBHm_Chr4g0445181
rosa_laevigata RLG00000005760
rosa_multiflora Rmu_sc0000050.1_g000037
rosa_roxburghii Rroxscaffold_5G00385690
rosa_rugosa Rorug04G0359700
rosa_samantha Rh4AG420500 Rh4BG432400 Rh4CG447000 Rh4DG428500
rosa_wichuraiana Rw4G036060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AarI CACCTGC 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 293
AccII CGCG 1 cut(s) 157
AciI CCGC 3 cut(s) 147, 155, 549
AclWI GGATC 5 cut(s) 90, 210, 223, 546, 814
AcoI YGGCCR 1 cut(s) 613
AcsI RAATTY 1 cut(s) 84
AcuI CTGAAG 1 cut(s) 298
AcvI CACGTG 1 cut(s) 516
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 3 cut(s) 662, 734, 897
AfeI AGCGCT 1 cut(s) 654
AfiI CCNNNNNNNGG 1 cut(s) 308
AflIII ACRYGT 1 cut(s) 515
AgsI TTSAA 4 cut(s) 21, 610, 670, 781
AjnI CCWGG 1 cut(s) 857
AleI CACNNNNGTG 1 cut(s) 533
AluBI AGCT 8 cut(s) 7, 427, 465, 492, 574, 585, 606, 743
AluI AGCT 8 cut(s) 7, 427, 465, 492, 574, 585, 606, 743
Alw21I GWGCWC 1 cut(s) 454
Alw44I GTGCAC 1 cut(s) 450
AlwI GGATC 5 cut(s) 90, 210, 223, 546, 814
Ama87I CYCGRG 2 cut(s) 542, 826
Aor51HI AGCGCT 1 cut(s) 654
AoxI GGCC 2 cut(s) 545, 613
ApaI GGGCCC 1 cut(s) 549
ApaLI GTGCAC 1 cut(s) 450
ApeKI GCWGC 4 cut(s) 144, 399, 462, 582
ApoI RAATTY 1 cut(s) 84
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AspLEI GCGC 2 cut(s) 159, 655
AspS9I GGNCC 3 cut(s) 545, 546, 855
AsuHPI GGTGA 4 cut(s) 187, 193, 220, 331
AvaI CYCGRG 2 cut(s) 542, 826
AvaII GGWCC 1 cut(s) 855
BaeGI GKGCMC 2 cut(s) 454, 549
BamHI GGATCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 549
BbrPI CACGTG 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 4 cut(s) 131, 411, 474, 594
BccI CCATC 4 cut(s) 365, 464, 515, 553
BceAI ACGGC 2 cut(s) 412, 600
BciT130I CCWGG 1 cut(s) 859
BfmI CTRYAG 2 cut(s) 460, 814
BfoI RGCGCY 1 cut(s) 656
BfuAI ACCTGC 1 cut(s) 293
BisI GCNGC 5 cut(s) 145, 148, 400, 463, 583
BlsI GCNGC 5 cut(s) 146, 149, 401, 464, 584
Bme1390I CCNGG 1 cut(s) 859
Bme18I GGWCC 1 cut(s) 855
BmeT110I CYCGRG 2 cut(s) 542, 826
BmgT120I GGNCC 3 cut(s) 545, 546, 855
BmiI GGNNCC 3 cut(s) 217, 547, 856
BmrFI CCNGG 1 cut(s) 859
BmsI GCATC 1 cut(s) 848
BpuEI CTTGAG 1 cut(s) 641
BsaAI YACGTR 1 cut(s) 516
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 212
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse118I RCCGGY 2 cut(s) 140, 532
BseBI CCWGG 1 cut(s) 859
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 3 cut(s) 13, 77, 389
BseLI CCNNNNNNNGG 1 cut(s) 308
BseMII CTCAG 1 cut(s) 279
BseRI GAGGAG 2 cut(s) 58, 727
BseSI GKGCMC 2 cut(s) 454, 549
BseXI GCAGC 4 cut(s) 131, 411, 474, 594
BseYI CCCAGC 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 277
Bsh1236I CGCG 1 cut(s) 157
BshFI GGCC 2 cut(s) 547, 615
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 2 cut(s) 542, 826
BsiSI CCGG 3 cut(s) 141, 213, 533
BslFI GGGAC 1 cut(s) 868
BslI CCNNNNNNNGG 1 cut(s) 308
BsmFI GGGAC 1 cut(s) 868
BsnI GGCC 2 cut(s) 547, 615
BsoBI CYCGRG 2 cut(s) 542, 826
Bsp120I GGGCCC 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 454, 549
Bsp143I GATC 4 cut(s) 95, 215, 538, 806
BspACI CCGC 3 cut(s) 147, 155, 549
BspANI GGCC 2 cut(s) 547, 615
BspCNI CTCAG 1 cut(s) 278
BspFNI CGCG 1 cut(s) 157
BspLI GGNNCC 3 cut(s) 217, 547, 856
BspMAI CTGCAG 1 cut(s) 464
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 5 cut(s) 90, 210, 223, 546, 814
BsrFI RCCGGY 2 cut(s) 140, 532
BssAI RCCGGY 2 cut(s) 140, 532
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 4 cut(s) 95, 215, 538, 806
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 1 cut(s) 859
BstACI GRCGYC 1 cut(s) 138
BstBAI YACGTR 1 cut(s) 516
BstC8I GCNNGC 2 cut(s) 142, 549
BstDEI CTNAG 2 cut(s) 8, 265
BstF5I GGATG 3 cut(s) 13, 77, 389
BstFNI CGCG 1 cut(s) 157
BstH2I RGCGCY 1 cut(s) 656
BstHHI GCGC 2 cut(s) 159, 655
BstKTI GATC 4 cut(s) 98, 218, 541, 809
BstMBI GATC 4 cut(s) 95, 215, 538, 806
BstMWI GCNNNNNNNGC 3 cut(s) 156, 408, 612
BstNI CCWGG 1 cut(s) 859
BstNSI RCATGY 1 cut(s) 180
BstSCI CCNGG 1 cut(s) 857
BstSFI CTRYAG 2 cut(s) 460, 814
BstSLI GKGCMC 2 cut(s) 454, 549
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 4 cut(s) 131, 411, 474, 594
BstX2I RGATCY 3 cut(s) 95, 215, 538
BstYI RGATCY 3 cut(s) 95, 215, 538
BsuRI GGCC 2 cut(s) 547, 615
BtgZI GCGATG 1 cut(s) 493
BtsCI GGATG 3 cut(s) 13, 77, 389
BtsI GCAGTG 2 cut(s) 139, 418
BtsIMutI CAGTG 2 cut(s) 139, 418
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 2 cut(s) 142, 549
CfoI GCGC 2 cut(s) 159, 655
Cfr10I RCCGGY 2 cut(s) 140, 532
Cfr13I GGNCC 3 cut(s) 545, 546, 855
CseI GACGC 2 cut(s) 146, 338
Csp6I GTAC 3 cut(s) 661, 733, 896
CviAII CATG 4 cut(s) 177, 277, 477, 696
CviQI GTAC 3 cut(s) 661, 733, 896
DdeI CTNAG 2 cut(s) 8, 265
DpnI GATC 4 cut(s) 97, 217, 540, 808
DpnII GATC 4 cut(s) 95, 215, 538, 806
EaeI YGGCCR 1 cut(s) 613
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 855
Eco47III AGCGCT 1 cut(s) 654
Eco57I CTGAAG 1 cut(s) 298
Eco72I CACGTG 1 cut(s) 516
Eco88I CYCGRG 2 cut(s) 542, 826
EcoO109I RGGNCCY 1 cut(s) 855
EcoRII CCWGG 1 cut(s) 857
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 4 cut(s) 180, 280, 480, 699
FaqI GGGAC 1 cut(s) 868
FatI CATG 4 cut(s) 176, 276, 476, 695
FauI CCCGC 2 cut(s) 162, 556
Fnu4HI GCNGC 5 cut(s) 145, 148, 400, 463, 583
FokI GGATG 2 cut(s) 64, 376
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 5 cut(s) 145, 148, 400, 463, 583
GlaI GCGC 2 cut(s) 158, 654
GluI GCNGC 5 cut(s) 145, 148, 400, 463, 583
GsaI CCCAGC 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 656
HaeIII GGCC 2 cut(s) 547, 615
HapII CCGG 3 cut(s) 141, 213, 533
HgaI GACGC 2 cut(s) 146, 338
HhaI GCGC 2 cut(s) 159, 655
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 4 cut(s) 180, 280, 480, 699
Hin6I GCGC 2 cut(s) 157, 653
HinP1I GCGC 2 cut(s) 157, 653
HindIII AAGCTT 2 cut(s) 5, 490
HinfI GANTC 2 cut(s) 108, 620
HpaII CCGG 3 cut(s) 141, 213, 533
HphI GGTGA 4 cut(s) 187, 193, 220, 331
Hpy166II GTNNAC 5 cut(s) 282, 339, 452, 526, 898
Hpy188I TCNGA 3 cut(s) 268, 625, 771
Hpy188III TCNNGA 1 cut(s) 556
Hpy8I GTNNAC 5 cut(s) 282, 339, 452, 526, 898
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 4 cut(s) 27, 46, 208, 352
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 9 cut(s) 102, 180, 294, 452, 462, 480, 568, 582, 839
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 408, 612
HpyF3I CTNAG 2 cut(s) 8, 265
HpySE526I ACGT 1 cut(s) 515
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 4 cut(s) 180, 280, 480, 699
HspAI GCGC 2 cut(s) 157, 653
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Kzo9I GATC 4 cut(s) 95, 215, 538, 806
LmnI GCTCC 4 cut(s) 293, 337, 383, 740
Lsp1109I GCAGC 4 cut(s) 131, 411, 474, 594
LweI GCATC 1 cut(s) 848
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 3 cut(s) 134, 172, 413
MalI GATC 4 cut(s) 97, 217, 540, 808
MboI GATC 4 cut(s) 95, 215, 538, 806
MboII GAAGA 5 cut(s) 34, 37, 43, 352, 494
MflI RGATCY 3 cut(s) 95, 215, 538
MhlI GDGCHC 2 cut(s) 454, 549
MluCI AATT 3 cut(s) 84, 703, 763
MmeI TCCRAC 3 cut(s) 417, 617, 648
MroNI GCCGGC 1 cut(s) 140
MseI TTAA 2 cut(s) 117, 702
MslI CAYNNNNRTG 1 cut(s) 533
MspI CCGG 3 cut(s) 141, 213, 533
MspR9I CCNGG 1 cut(s) 859
MvaI CCWGG 1 cut(s) 859
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 3 cut(s) 156, 408, 612
NaeI GCCGGC 1 cut(s) 142
NdeII GATC 4 cut(s) 95, 215, 538, 806
NgoMIV GCCGGC 1 cut(s) 140
NlaIII CATG 4 cut(s) 180, 280, 480, 699
NlaIV GGNNCC 3 cut(s) 217, 547, 856
NmuCI GTSAC 2 cut(s) 134, 413
NspI RCATGY 1 cut(s) 180
OliI CACNNNNGTG 1 cut(s) 533
PaeR7I CTCGAG 1 cut(s) 826
PaqCI CACCTGC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PdiI GCCGGC 1 cut(s) 142
PfeI GAWTC 2 cut(s) 108, 620
PkrI GCNGC 5 cut(s) 146, 149, 401, 464, 584
PmaCI CACGTG 1 cut(s) 516
PmlI CACGTG 1 cut(s) 516
Ppu21I YACGTR 1 cut(s) 516
PpuMI RGGWCCY 1 cut(s) 855
PsiI TTATAA 1 cut(s) 747
Psp5II RGGWCCY 1 cut(s) 855
Psp6I CCWGG 1 cut(s) 857
PspCI CACGTG 1 cut(s) 516
PspFI CCCAGC 1 cut(s) 302
PspGI CCWGG 1 cut(s) 857
PspN4I GGNNCC 3 cut(s) 217, 547, 856
PspOMI GGGCCC 1 cut(s) 545
PspPI GGNCC 3 cut(s) 545, 546, 855
PspPPI RGGWCCY 1 cut(s) 855
PspXI VCTCGAGB 1 cut(s) 826
PstI CTGCAG 1 cut(s) 464
PsuI RGATCY 3 cut(s) 95, 215, 538
RsaI GTAC 3 cut(s) 662, 734, 897
RsaNI GTAC 3 cut(s) 661, 733, 896
RseI CAYNNNNRTG 1 cut(s) 533
SaqAI TTAA 2 cut(s) 117, 702
SatI GCNGC 5 cut(s) 145, 148, 400, 463, 583
Sau3AI GATC 4 cut(s) 95, 215, 538, 806
Sau96I GGNCC 3 cut(s) 545, 546, 855
ScrFI CCNGG 1 cut(s) 859
SduI GDGCHC 2 cut(s) 454, 549
SfaNI GCATC 1 cut(s) 848
SfcI CTRYAG 2 cut(s) 460, 814
Sfr274I CTCGAG 1 cut(s) 826
SgrAI CRCCGGYG 1 cut(s) 532
SinI GGWCC 1 cut(s) 855
SlaI CTCGAG 1 cut(s) 826
SmiMI CAYNNNNRTG 1 cut(s) 533
SmlI CTYRAG 2 cut(s) 656, 826
SmoI CTYRAG 2 cut(s) 656, 826
Sse9I AATT 3 cut(s) 84, 703, 763
SsiI CCGC 3 cut(s) 147, 155, 549
StyD4I CCNGG 1 cut(s) 857
StyI CCWWGG 1 cut(s) 445
TaiI ACGT 1 cut(s) 518
TaqI TCGA 3 cut(s) 372, 555, 827
TasI AATT 3 cut(s) 84, 703, 763
TatI WGTACW 2 cut(s) 660, 895
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 2 cut(s) 108, 620
Tru1I TTAA 2 cut(s) 117, 702
Tru9I TTAA 2 cut(s) 117, 702
TscAI CASTG 2 cut(s) 139, 418
TseFI GTSAC 2 cut(s) 134, 413
TseI GCWGC 4 cut(s) 144, 399, 462, 582
Tsp45I GTSAC 2 cut(s) 134, 413
TspDTI ATGAA 6 cut(s) 17, 28, 37, 465, 484, 684
TspGWI ACGGA 2 cut(s) 160, 324
TspRI CASTG 2 cut(s) 139, 418
VneI GTGCAC 1 cut(s) 450
VpaK11BI GGWCC 1 cut(s) 855
XapI RAATTY 1 cut(s) 84
XceI RCATGY 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.