pycom16g07030

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012438
Physical Location & Seq
Forward (+)
4560959 .. 4561612
654 bp
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UTR
Exon/CDS
Intron
pycom16g07030.1

Sequence Viewer

Length: 654 bp
ATGAAGTTTAGCTCTCCTTCAACACCAACTCTCTCCATCACCTTCCTCTCCATCTTCCTCCTCCAACTCACCTTCATCTCCTCCCAAACCTGCCAAAGATCCTGCGGCGAACTCCCCCTCAAGTTCCCATTCGGCAGCGACCCCGGCTGTGGCGACCCGCGCTTCAACCCTCACGTGTCGTGCAGCCAGGGAAACCCCATCTTCACAACCCACAGCGGATCCTACCCCATCACCCACATAGACTACACAAACAAGGTCATGTACATCTCAGACCCCTCCATGTCAACATGTTCATGCAAACAACAATCCAAAGGGTTTGCCCTAGACTGGGACGCACCCTTCGATTTCCAAGACGACAACGTTTTTGCCCTACTCGACTGCTCACTTTCCACCTCTCCAATCTTCCAACAAAACTCCCTATACACTGACAAAAAAAACAGCTCAAAAATCACCCTATGTGCCAACCAAGGTGCCCCGATTTGTAGCTACTTGTACTCATGTCAGGCGATCAGCACAATCAACCTCCCGACATCCACCTGTTGTGTTTACACGCCGGTGGATCTCGGGCCGGCTTTCGAGATGGATTTGGAGAAGCTGCAGTGCAGCTCTTACTCAGGGTTTTACAGCTTCAACGAACGTGAGTCTCGATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

218

Amino Acids

23.98

Weight (kDa)

5.03

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 91 8.3e-12 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10380 AT1G10380
fragaria_vesca FvH4_4g35530
malus_domestica MD13G1082400.v1.1 MD16G1081500.v1.1
prunus_persica Prupe.3G121800_v2.0.a1 Prupe.3G121800_v2.0.a1
pyrus_communis pycom13g07100 pycom16g07030
rosa_chinensis RchiOBHm_Chr4g0445181
rosa_laevigata RLG00000005760
rosa_multiflora Rmu_sc0000050.1_g000037
rosa_roxburghii Rroxscaffold_5G00385690
rosa_rugosa Rorug04G0359700
rosa_samantha Rh4AG420500 Rh4BG432400 Rh4CG447000 Rh4DG428500
rosa_wichuraiana Rw4G036060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 470
AccII CGCG 1 cut(s) 160
AciI CCGC 3 cut(s) 105, 158, 216
AclI AACGTT 1 cut(s) 360
AclWI GGATC 5 cut(s) 93, 213, 226, 567, 642
AcvI CACGTG 1 cut(s) 175
AfaI GTAC 2 cut(s) 263, 494
AfiI CCNNNNNNNGG 3 cut(s) 149, 327, 328
AflIII ACRYGT 2 cut(s) 174, 287
AgsI TTSAA 3 cut(s) 21, 166, 631
AjnI CCWGG 1 cut(s) 186
AleI CACNNNNGTG 1 cut(s) 554
AluBI AGCT 6 cut(s) 12, 441, 486, 595, 606, 627
AluI AGCT 6 cut(s) 12, 441, 486, 595, 606, 627
Alw26I GTCTC 1 cut(s) 648
AlwI GGATC 5 cut(s) 93, 213, 226, 567, 642
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 1 cut(s) 566
ApeKI GCWGC 4 cut(s) 135, 183, 595, 603
ArsI GACNNNNNNTTYG 4 cut(s) 323, 347, 355, 379
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 1 cut(s) 566
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 4 cut(s) 31, 61, 223, 442
AvaI CYCGRG 1 cut(s) 563
BaeGI GKGCMC 1 cut(s) 475
BamHI GGATCC 1 cut(s) 218
BanI GGYRCC 1 cut(s) 470
BbrPI CACGTG 1 cut(s) 175
BbvI GCAGC 4 cut(s) 147, 195, 582, 615
BccI CCATC 5 cut(s) 44, 59, 206, 236, 574
BciT130I CCWGG 1 cut(s) 188
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 648
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 596
BfuAI ACCTGC 1 cut(s) 98
BisI GCNGC 5 cut(s) 106, 136, 184, 596, 604
BlsI GCNGC 5 cut(s) 107, 137, 185, 597, 605
Bme1390I CCNGG 2 cut(s) 144, 188
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 566
BmiI GGNNCC 2 cut(s) 220, 472
BmrFI CCNGG 2 cut(s) 144, 188
BmrI ACTGGG 1 cut(s) 337
BmuI ACTGGG 1 cut(s) 337
BpuEI CTTGAG 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 175
BsaJI CCNNGG 3 cut(s) 142, 187, 466
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 327, 328
Bse118I RCCGGY 2 cut(s) 553, 568
Bse1I ACTGG 1 cut(s) 332
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 3 cut(s) 142, 187, 466
BseGI GGATG 1 cut(s) 530
BseLI CCNNNNNNNGG 3 cut(s) 149, 327, 328
BseMII CTCAG 2 cut(s) 282, 627
BseNI ACTGG 1 cut(s) 332
BseRI GAGGAG 2 cut(s) 50, 70
BseSI GKGCMC 1 cut(s) 475
BseXI GCAGC 4 cut(s) 147, 195, 582, 615
BsgI GTGCAG 2 cut(s) 202, 622
Bsh1236I CGCG 1 cut(s) 160
BshFI GGCC 1 cut(s) 568
BshNI GGYRCC 1 cut(s) 470
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 3 cut(s) 144, 554, 569
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 3 cut(s) 149, 327, 328
BsmAI GTCTC 1 cut(s) 648
BsmFI GGGAC 1 cut(s) 344
BsnI GGCC 1 cut(s) 568
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 475
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 5 cut(s) 98, 218, 507, 559, 647
BspACI CCGC 3 cut(s) 105, 158, 216
BspANI GGCC 1 cut(s) 568
BspCNI CTCAG 2 cut(s) 281, 626
BspFNI CGCG 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 220, 472
BspMAI CTGCAG 1 cut(s) 600
BspMI ACCTGC 1 cut(s) 98
BspPI GGATC 5 cut(s) 93, 213, 226, 567, 642
BspT107I GGYRCC 1 cut(s) 470
BsrFI RCCGGY 2 cut(s) 553, 568
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 1 cut(s) 332
BssAI RCCGGY 2 cut(s) 553, 568
BssECI CCNNGG 3 cut(s) 142, 187, 466
BssMI GATC 5 cut(s) 98, 218, 507, 559, 647
BssT1I CCWWGG 1 cut(s) 466
Bst2UI CCWGG 1 cut(s) 188
BstAUI TGTACA 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 570
BstDEI CTNAG 2 cut(s) 268, 613
BstF5I GGATG 1 cut(s) 530
BstFNI CGCG 1 cut(s) 160
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 5 cut(s) 101, 221, 510, 562, 650
BstMAI GTCTC 1 cut(s) 648
BstMBI GATC 5 cut(s) 98, 218, 507, 559, 647
BstMWI GCNNNNNNNGC 2 cut(s) 144, 159
BstNI CCWGG 1 cut(s) 188
BstNSI RCATGY 1 cut(s) 291
BstSCI CCNGG 2 cut(s) 142, 186
BstSFI CTRYAG 1 cut(s) 596
BstSLI GKGCMC 1 cut(s) 475
BstUI CGCG 1 cut(s) 160
BstV1I GCAGC 4 cut(s) 147, 195, 582, 615
BstX2I RGATCY 3 cut(s) 98, 218, 559
BstYI RGATCY 3 cut(s) 98, 218, 559
BsuRI GGCC 1 cut(s) 568
BtsCI GGATG 1 cut(s) 530
BtsI GCAGTG 1 cut(s) 605
BtsIMutI CAGTG 2 cut(s) 423, 605
BveI ACCTGC 1 cut(s) 98
Cac8I GCNNGC 1 cut(s) 570
CfoI GCGC 1 cut(s) 162
Cfr10I RCCGGY 2 cut(s) 553, 568
Cfr13I GGNCC 1 cut(s) 566
CseI GACGC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 262, 493
CviAII CATG 5 cut(s) 259, 280, 288, 294, 498
CviQI GTAC 2 cut(s) 262, 493
DdeI CTNAG 2 cut(s) 268, 613
DpnI GATC 5 cut(s) 100, 220, 509, 561, 649
DpnII GATC 5 cut(s) 98, 218, 507, 559, 647
Eco130I CCWWGG 1 cut(s) 466
Eco72I CACGTG 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 563
EcoRII CCWGG 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 5 cut(s) 262, 283, 291, 297, 501
FaiI YATR 8 cut(s) 239, 260, 281, 289, 295, 421, 457, 499
FaqI GGGAC 1 cut(s) 344
FatI CATG 5 cut(s) 258, 279, 287, 293, 497
FauI CCCGC 1 cut(s) 165
Fnu4HI GCNGC 5 cut(s) 106, 136, 184, 596, 604
FokI GGATG 1 cut(s) 517
Fsp4HI GCNGC 5 cut(s) 106, 136, 184, 596, 604
FspBI CTAG 1 cut(s) 323
GlaI GCGC 1 cut(s) 161
GluI GCNGC 5 cut(s) 106, 136, 184, 596, 604
HaeIII GGCC 1 cut(s) 568
HapII CCGG 3 cut(s) 144, 554, 569
HgaI GACGC 1 cut(s) 341
HhaI GCGC 1 cut(s) 162
Hin1II CATG 5 cut(s) 262, 283, 291, 297, 501
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 1 cut(s) 641
HpaII CCGG 3 cut(s) 144, 554, 569
HphI GGTGA 4 cut(s) 31, 61, 223, 442
Hpy166II GTNNAC 2 cut(s) 285, 547
Hpy188I TCNGA 1 cut(s) 271
Hpy188III TCNNGA 3 cut(s) 526, 577, 645
Hpy8I GTNNAC 2 cut(s) 285, 547
HpyAV CCTTC 4 cut(s) 27, 52, 82, 349
HpyCH4IV ACGT 3 cut(s) 174, 360, 637
HpyCH4V TGCA 4 cut(s) 183, 297, 598, 603
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 159
HpyF3I CTNAG 2 cut(s) 268, 613
HpySE526I ACGT 3 cut(s) 174, 360, 637
Hsp92II CATG 5 cut(s) 262, 283, 291, 297, 501
HspAI GCGC 1 cut(s) 160
KroI GCCGGC 1 cut(s) 568
KroNI GCCGGC 1 cut(s) 570
Kzo9I GATC 5 cut(s) 98, 218, 507, 559, 647
Lsp1109I GCAGC 4 cut(s) 147, 195, 582, 615
MaeI CTAG 1 cut(s) 323
MaeII ACGT 3 cut(s) 174, 360, 637
MalI GATC 5 cut(s) 100, 220, 509, 561, 649
MboI GATC 5 cut(s) 98, 218, 507, 559, 647
MboII GAAGA 3 cut(s) 46, 193, 394
MflI RGATCY 3 cut(s) 98, 218, 559
MhlI GDGCHC 1 cut(s) 475
MlyI GAGTC 1 cut(s) 650
MmeI TCCRAC 2 cut(s) 88, 430
MnlI CCTC 9 cut(s) 56, 68, 71, 91, 128, 180, 286, 403, 533
MroNI GCCGGC 1 cut(s) 568
MslI CAYNNNNRTG 2 cut(s) 292, 554
MspA1I CMGCKG 1 cut(s) 216
MspI CCGG 3 cut(s) 144, 554, 569
MspR9I CCNGG 2 cut(s) 144, 188
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 2 cut(s) 144, 159
NaeI GCCGGC 1 cut(s) 570
NciI CCSGG 1 cut(s) 144
NdeII GATC 5 cut(s) 98, 218, 507, 559, 647
NgoMIV GCCGGC 1 cut(s) 568
NlaIII CATG 5 cut(s) 262, 283, 291, 297, 501
NlaIV GGNNCC 2 cut(s) 220, 472
NspI RCATGY 1 cut(s) 291
OliI CACNNNNGTG 1 cut(s) 554
PciI ACATGT 1 cut(s) 287
PcsI WCGNNNNNNNCGW 2 cut(s) 339, 643
PdiI GCCGGC 1 cut(s) 570
PkrI GCNGC 5 cut(s) 107, 137, 185, 597, 605
PleI GAGTC 1 cut(s) 649
PmaCI CACGTG 1 cut(s) 175
PmlI CACGTG 1 cut(s) 175
PpsI GAGTC 1 cut(s) 649
Ppu21I YACGTR 1 cut(s) 175
PscI ACATGT 1 cut(s) 287
Psp1406I AACGTT 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 186
PspCI CACGTG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 2 cut(s) 220, 472
PspPI GGNCC 1 cut(s) 566
PstI CTGCAG 1 cut(s) 600
PsuI RGATCY 3 cut(s) 98, 218, 559
RsaI GTAC 2 cut(s) 263, 494
RsaNI GTAC 2 cut(s) 262, 493
RseI CAYNNNNRTG 2 cut(s) 292, 554
SatI GCNGC 5 cut(s) 106, 136, 184, 596, 604
Sau3AI GATC 5 cut(s) 98, 218, 507, 559, 647
Sau96I GGNCC 1 cut(s) 566
SchI GAGTC 1 cut(s) 650
ScrFI CCNGG 2 cut(s) 144, 188
SduI GDGCHC 1 cut(s) 475
SfcI CTRYAG 1 cut(s) 596
SgrAI CRCCGGYG 1 cut(s) 553
SmiMI CAYNNNNRTG 2 cut(s) 292, 554
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
SsiI CCGC 3 cut(s) 105, 158, 216
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 2 cut(s) 142, 186
StyI CCWWGG 1 cut(s) 466
TaiI ACGT 3 cut(s) 177, 363, 640
TaqI TCGA 4 cut(s) 342, 375, 576, 646
TatI WGTACW 2 cut(s) 261, 492
TauI GCSGC 1 cut(s) 108
TscAI CASTG 2 cut(s) 430, 605
TseI GCWGC 4 cut(s) 135, 183, 595, 603
TspDTI ATGAA 3 cut(s) 17, 64, 282
TspRI CASTG 2 cut(s) 430, 605
XceI RCATGY 1 cut(s) 291
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.