pycom13g07100

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012438
Physical Location & Seq
Forward (+)
4703955 .. 4705026
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g07100.1

Sequence Viewer

Length: 921 bp
ATGAAGTTTAGCTCTCCTTCAACATCAACTCTCTCCATCATCTTCCTCTCCACCTTCCTCCTCCAACTCACCTTCATCTCCTCCCAAATCTGCCAAAGATCCTGCGGCAAACTCCCCCTCAAGTTCCCATTCGGCACCGACCCCGGCTGCGGCGACCCGGGCTTCAACCCCCACGTGTCATGCAGCCAGGGAAACCCTATCTTCACAACCCACACCGGATCCTACCCCATCACCAACATAGACTACACAAAGAAAGTTATGTACATCTCAGACCCCTCCATGTCAACATGTTCATGCAACCAACAATCCAAAGGTTTTTCCCTAGACTGGGACGCACCCTTCAGTTTCCAAGACGACAACGTTTTTGCCCTACTCGACTGCTCGCTTTCCAACTCTCCAGTCTTCCAGCAAAACTCCCTATATACAGACCAAAAAAACAACTCTAAAGTGTCCCTATGTGACAACCAAGGTGCTCCGATTTGCAGCTTCTTGTACTCGTGCCAGGCGATCAGCACGATCAACCTCCCGATATCCACGTGTTGCGTTTACACGCCGGTGGATCTCGGGCCGGCTTTCGAGATGGATTTGGAGAAGCTGCAGTGCAGTTCTTACTCAGGGTTTTACAGCTTCAACGAACATGAGTCTGACCCAAATAGTTGGAACTACGGGATTGCCCTCAAGTACAAGTTCAGTGTGAACAACGAGTATCCGGATTCGTGTGTTAATTGTGAGAGGAGTAATGGGGTTTGTGGGTATTATGGTAATTACAACTCGTTTATATGTAATTGTCCGAATGGGATCAACACTACCAATGATTGCTTTTTTGGAGCTTCTTATAACTATGGTTCAAGGCTTCGATCGTCGCAGATTGGGACTTGGTTAATCTATTGCTTGGCTTGGCTATTGGTTTTGGTATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

307

Amino Acids

33.94

Weight (kDa)

4.82

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 91 5.8e-12 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 211 - 265 4.2e-08 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10380 AT1G10380
fragaria_vesca FvH4_4g35530
malus_domestica MD13G1082400.v1.1 MD16G1081500.v1.1
prunus_persica Prupe.3G121800_v2.0.a1 Prupe.3G121800_v2.0.a1
pyrus_communis pycom13g07100 pycom16g07030
rosa_chinensis RchiOBHm_Chr4g0445181
rosa_laevigata RLG00000005760
rosa_multiflora Rmu_sc0000050.1_g000037
rosa_roxburghii Rroxscaffold_5G00385690
rosa_rugosa Rorug04G0359700
rosa_samantha Rh4AG420500 Rh4BG432400 Rh4CG447000 Rh4DG428500
rosa_wichuraiana Rw4G036060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 837
AccB1I GGYRCC 1 cut(s) 134
AccIII TCCGGA 1 cut(s) 709
AciI CCGC 2 cut(s) 105, 150
AclI AACGTT 1 cut(s) 360
AclWI GGATC 5 cut(s) 93, 213, 226, 567, 806
AcuI CTGAAG 1 cut(s) 325
AcvI CACGTG 2 cut(s) 175, 537
AfaI GTAC 3 cut(s) 263, 494, 683
AfiI CCNNNNNNNGG 3 cut(s) 149, 327, 328
AflIII ACRYGT 3 cut(s) 174, 287, 536
AgsI TTSAA 4 cut(s) 21, 166, 631, 849
AjnI CCWGG 2 cut(s) 186, 501
AleI CACNNNNGTG 1 cut(s) 554
AluBI AGCT 5 cut(s) 12, 486, 595, 627, 830
AluI AGCT 5 cut(s) 12, 486, 595, 627, 830
Alw21I GWGCWC 1 cut(s) 475
AlwI GGATC 5 cut(s) 93, 213, 226, 567, 806
Ama87I CYCGRG 2 cut(s) 157, 563
Aor13HI TCCGGA 1 cut(s) 709
AoxI GGCC 1 cut(s) 566
ApeKI GCWGC 4 cut(s) 147, 183, 483, 595
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AspS9I GGNCC 1 cut(s) 566
AsuC2I CCSGG 3 cut(s) 144, 158, 159
AsuHPI GGTGA 2 cut(s) 61, 223
AvaI CYCGRG 2 cut(s) 157, 563
BamHI GGATCC 1 cut(s) 218
BanI GGYRCC 1 cut(s) 134
BauI CACGAG 1 cut(s) 496
BbrPI CACGTG 2 cut(s) 175, 537
BbsI GAAGAC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 4 cut(s) 134, 195, 495, 582
BccI CCATC 3 cut(s) 44, 236, 574
BciT130I CCWGG 2 cut(s) 188, 503
BciVI GTATCC 1 cut(s) 717
BcnI CCSGG 3 cut(s) 144, 158, 159
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 596
BfuI GTATCC 1 cut(s) 717
BisI GCNGC 6 cut(s) 106, 148, 151, 184, 484, 596
BlsI GCNGC 6 cut(s) 107, 149, 152, 185, 485, 597
Bme1390I CCNGG 5 cut(s) 144, 158, 159, 188, 503
BmeT110I CYCGRG 2 cut(s) 157, 563
BmgT120I GGNCC 1 cut(s) 566
BmiI GGNNCC 2 cut(s) 136, 220
BmrFI CCNGG 5 cut(s) 144, 158, 159, 188, 503
BmrI ACTGGG 1 cut(s) 337
BmuI ACTGGG 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 394
BpmI CTGGAG 1 cut(s) 381
BpuEI CTTGAG 2 cut(s) 104, 662
BpuMI CCSGG 3 cut(s) 144, 158, 159
BsaAI YACGTR 2 cut(s) 175, 537
BsaJI CCNNGG 4 cut(s) 142, 157, 187, 466
BsaWI WCCGGW 2 cut(s) 215, 709
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 327, 328
Bse118I RCCGGY 2 cut(s) 553, 568
Bse1I ACTGG 2 cut(s) 332, 398
BseAI TCCGGA 1 cut(s) 709
BseBI CCWGG 2 cut(s) 188, 503
BseDI CCNNGG 4 cut(s) 142, 157, 187, 466
BseLI CCNNNNNNNGG 3 cut(s) 149, 327, 328
BseMII CTCAG 2 cut(s) 282, 627
BseNI ACTGG 2 cut(s) 332, 398
BseRI GAGGAG 3 cut(s) 50, 70, 748
BseXI GCAGC 4 cut(s) 134, 195, 495, 582
BsgI GTGCAG 1 cut(s) 622
Bsh1285I CGRYCG 1 cut(s) 860
BshFI GGCC 1 cut(s) 568
BshNI GGYRCC 1 cut(s) 134
BsiEI CGRYCG 1 cut(s) 860
BsiHKAI GWGCWC 1 cut(s) 475
BsiHKCI CYCGRG 2 cut(s) 157, 563
BsiSI CCGG 6 cut(s) 144, 158, 216, 554, 569, 710
BslFI GGGAC 3 cut(s) 344, 436, 886
BslI CCNNNNNNNGG 3 cut(s) 149, 327, 328
BsmFI GGGAC 3 cut(s) 344, 436, 886
BsnI GGCC 1 cut(s) 568
BsoBI CYCGRG 2 cut(s) 157, 563
Bsp1286I GDGCHC 1 cut(s) 475
Bsp13I TCCGGA 1 cut(s) 709
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 7 cut(s) 98, 218, 507, 516, 559, 798, 857
BspACI CCGC 2 cut(s) 105, 150
BspANI GGCC 1 cut(s) 568
BspCNI CTCAG 2 cut(s) 281, 626
BspEI TCCGGA 1 cut(s) 709
BspLI GGNNCC 2 cut(s) 136, 220
BspMAI CTGCAG 1 cut(s) 600
BspPI GGATC 5 cut(s) 93, 213, 226, 567, 806
BspT107I GGYRCC 1 cut(s) 134
BsrFI RCCGGY 2 cut(s) 553, 568
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 2 cut(s) 332, 398
BssAI RCCGGY 2 cut(s) 553, 568
BssECI CCNNGG 4 cut(s) 142, 157, 187, 466
BssMI GATC 7 cut(s) 98, 218, 507, 516, 559, 798, 857
BssSI CACGAG 1 cut(s) 496
BssT1I CCWWGG 1 cut(s) 466
Bst2BI CACGAG 1 cut(s) 496
Bst2UI CCWGG 2 cut(s) 188, 503
BstAUI TGTACA 1 cut(s) 261
BstBAI YACGTR 2 cut(s) 175, 537
BstC8I GCNNGC 2 cut(s) 383, 570
BstDEI CTNAG 2 cut(s) 268, 613
BstKTI GATC 7 cut(s) 101, 221, 510, 519, 562, 801, 860
BstMBI GATC 7 cut(s) 98, 218, 507, 516, 559, 798, 857
BstMCI CGRYCG 1 cut(s) 860
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 2 cut(s) 188, 503
BstNSI RCATGY 1 cut(s) 291
BstSCI CCNGG 5 cut(s) 142, 156, 157, 186, 501
BstSFI CTRYAG 1 cut(s) 596
BstV1I GCAGC 4 cut(s) 134, 195, 495, 582
BstV2I GAAGAC 1 cut(s) 394
BstX2I RGATCY 3 cut(s) 98, 218, 559
BstXI CCANNNNNNTGG 1 cut(s) 657
BstYI RGATCY 3 cut(s) 98, 218, 559
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 1 cut(s) 568
BtsI GCAGTG 1 cut(s) 605
BtsIMutI CAGTG 2 cut(s) 605, 697
Cac8I GCNNGC 2 cut(s) 383, 570
Cfr10I RCCGGY 2 cut(s) 553, 568
Cfr13I GGNCC 1 cut(s) 566
Cfr9I CCCGGG 1 cut(s) 157
CseI GACGC 1 cut(s) 341
Csp6I GTAC 3 cut(s) 262, 493, 682
CviAII CATG 5 cut(s) 180, 280, 288, 294, 638
CviQI GTAC 3 cut(s) 262, 493, 682
DdeI CTNAG 2 cut(s) 268, 613
DpnI GATC 7 cut(s) 100, 220, 509, 518, 561, 800, 859
DpnII GATC 7 cut(s) 98, 218, 507, 516, 559, 798, 857
Eco130I CCWWGG 1 cut(s) 466
Eco32I GATATC 1 cut(s) 531
Eco57I CTGAAG 1 cut(s) 325
Eco72I CACGTG 2 cut(s) 175, 537
Eco88I CYCGRG 2 cut(s) 157, 563
EcoRII CCWGG 2 cut(s) 186, 501
EcoRV GATATC 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 5 cut(s) 183, 283, 291, 297, 641
FaqI GGGAC 3 cut(s) 344, 436, 886
FatI CATG 5 cut(s) 179, 279, 287, 293, 637
Fnu4HI GCNGC 6 cut(s) 106, 148, 151, 184, 484, 596
Fsp4HI GCNGC 6 cut(s) 106, 148, 151, 184, 484, 596
FspBI CTAG 1 cut(s) 323
GluI GCNGC 6 cut(s) 106, 148, 151, 184, 484, 596
GsuI CTGGAG 1 cut(s) 381
HaeIII GGCC 1 cut(s) 568
HapII CCGG 6 cut(s) 144, 158, 216, 554, 569, 710
HgaI GACGC 1 cut(s) 341
Hin1II CATG 5 cut(s) 183, 283, 291, 297, 641
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 2 cut(s) 641, 713
HpaII CCGG 6 cut(s) 144, 158, 216, 554, 569, 710
HphI GGTGA 2 cut(s) 61, 223
Hpy166II GTNNAC 3 cut(s) 285, 547, 697
Hpy188I TCNGA 4 cut(s) 271, 477, 646, 792
Hpy188III TCNNGA 3 cut(s) 526, 577, 710
Hpy8I GTNNAC 3 cut(s) 285, 547, 697
Hpy99I CGWCG 1 cut(s) 865
HpyAV CCTTC 4 cut(s) 27, 64, 82, 349
HpyCH4IV ACGT 3 cut(s) 174, 360, 536
HpyCH4V TGCA 5 cut(s) 183, 297, 483, 598, 603
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 2 cut(s) 268, 613
HpySE526I ACGT 3 cut(s) 174, 360, 536
Hsp92II CATG 5 cut(s) 183, 283, 291, 297, 641
Kpn2I TCCGGA 1 cut(s) 709
KroI GCCGGC 1 cut(s) 568
KroNI GCCGGC 1 cut(s) 570
Kzo9I GATC 7 cut(s) 98, 218, 507, 516, 559, 798, 857
LmnI GCTCC 2 cut(s) 478, 827
Lsp1109I GCAGC 4 cut(s) 134, 195, 495, 582
MaeI CTAG 1 cut(s) 323
MaeII ACGT 3 cut(s) 174, 360, 536
MaeIII GTNAC 1 cut(s) 458
MalI GATC 7 cut(s) 100, 220, 509, 518, 561, 800, 859
MboI GATC 7 cut(s) 98, 218, 507, 516, 559, 798, 857
MboII GAAGA 3 cut(s) 34, 193, 394
MflI RGATCY 3 cut(s) 98, 218, 559
MhlI GDGCHC 1 cut(s) 475
MluCI AATT 3 cut(s) 724, 763, 784
MlyI GAGTC 1 cut(s) 650
MmeI TCCRAC 3 cut(s) 88, 414, 638
MnlI CCTC 9 cut(s) 56, 68, 71, 91, 128, 286, 533, 686, 726
MroI TCCGGA 1 cut(s) 709
MroNI GCCGGC 1 cut(s) 568
MseI TTAA 2 cut(s) 723, 881
MslI CAYNNNNRTG 2 cut(s) 292, 554
MspI CCGG 6 cut(s) 144, 158, 216, 554, 569, 710
MspR9I CCNGG 5 cut(s) 144, 158, 159, 188, 503
MvaI CCWGG 2 cut(s) 188, 503
MwoI GCNNNNNNNGC 1 cut(s) 159
NaeI GCCGGC 1 cut(s) 570
NciI CCSGG 3 cut(s) 144, 158, 159
NdeII GATC 7 cut(s) 98, 218, 507, 516, 559, 798, 857
NgoMIV GCCGGC 1 cut(s) 568
NlaIII CATG 5 cut(s) 183, 283, 291, 297, 641
NlaIV GGNNCC 2 cut(s) 136, 220
NmuCI GTSAC 1 cut(s) 458
NspI RCATGY 1 cut(s) 291
OliI CACNNNNGTG 1 cut(s) 554
PciI ACATGT 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 503
PdiI GCCGGC 1 cut(s) 570
PfeI GAWTC 1 cut(s) 713
PkrI GCNGC 6 cut(s) 107, 149, 152, 185, 485, 597
Ple19I CGATCG 1 cut(s) 860
PleI GAGTC 1 cut(s) 649
PmaCI CACGTG 2 cut(s) 175, 537
PmlI CACGTG 2 cut(s) 175, 537
PpsI GAGTC 1 cut(s) 649
Ppu21I YACGTR 2 cut(s) 175, 537
PscI ACATGT 1 cut(s) 287
PsiI TTATAA 1 cut(s) 837
Psp1406I AACGTT 1 cut(s) 360
Psp6I CCWGG 2 cut(s) 186, 501
PspCI CACGTG 2 cut(s) 175, 537
PspGI CCWGG 2 cut(s) 186, 501
PspN4I GGNNCC 2 cut(s) 136, 220
PspPI GGNCC 1 cut(s) 566
PstI CTGCAG 1 cut(s) 600
PsuI RGATCY 3 cut(s) 98, 218, 559
PvuI CGATCG 1 cut(s) 860
RsaI GTAC 3 cut(s) 263, 494, 683
RsaNI GTAC 3 cut(s) 262, 493, 682
RseI CAYNNNNRTG 2 cut(s) 292, 554
SaqAI TTAA 2 cut(s) 723, 881
SatI GCNGC 6 cut(s) 106, 148, 151, 184, 484, 596
Sau3AI GATC 7 cut(s) 98, 218, 507, 516, 559, 798, 857
Sau96I GGNCC 1 cut(s) 566
SchI GAGTC 1 cut(s) 650
ScrFI CCNGG 5 cut(s) 144, 158, 159, 188, 503
SduI GDGCHC 1 cut(s) 475
SfcI CTRYAG 1 cut(s) 596
SgrAI CRCCGGYG 1 cut(s) 553
SmaI CCCGGG 1 cut(s) 159
SmiMI CAYNNNNRTG 2 cut(s) 292, 554
SmlI CTYRAG 2 cut(s) 119, 677
SmoI CTYRAG 2 cut(s) 119, 677
Sse9I AATT 3 cut(s) 724, 763, 784
SsiI CCGC 2 cut(s) 105, 150
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 5 cut(s) 142, 156, 157, 186, 501
StyI CCWWGG 1 cut(s) 466
TaiI ACGT 3 cut(s) 177, 363, 539
TaqI TCGA 3 cut(s) 375, 576, 856
TasI AATT 3 cut(s) 724, 763, 784
TatI WGTACW 3 cut(s) 261, 492, 681
TauI GCSGC 2 cut(s) 108, 153
TfiI GAWTC 1 cut(s) 713
Tru1I TTAA 2 cut(s) 723, 881
Tru9I TTAA 2 cut(s) 723, 881
TscAI CASTG 2 cut(s) 605, 697
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 4 cut(s) 147, 183, 483, 595
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 3 cut(s) 17, 64, 282
TspMI CCCGGG 1 cut(s) 157
TspRI CASTG 2 cut(s) 605, 697
XceI RCATGY 1 cut(s) 291
XmaI CCCGGG 1 cut(s) 157
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.