MD16G1081500.v1.1

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012438
Physical Location & Seq
Forward (+)
5714201 .. 5715520
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1081500.v1.1.491

Sequence Viewer

Length: 921 bp
ATGAAGTTTAGCTCTCCTTCAACACCAACTCTCTCCATCACCTTCCTCTCCATCTTCCTCCTCCAACTCACCTTCATCTCCTCCCAAACCTGCCAAAGATCCTGCGGCGAACTCCCCCTCAAGTTCCCATTCGGCAGCGACCCCGGCTGTGGCGACGCGCGCTTCAACCCTCACGTGTCGTGCAGCCAGGGAAACCCCATCTTCACAACCCACACCGGATCCTACCCCATCACGAACATAGACTACACAAAGAAGGTCATGTACATCTCAGACCCCTCCATGTCAACATGTTCATGCAAACAACAATCCAAAGGGTTTGCCCTAGACTGGGACGCACCCTTCGATTTCCAGGACGACAACGTTTTTGCCCTACTCGACTGCTCACTTTCCACCTCTCCAATCTTCCAACAAAACTCCCTATACACTGACAAAAAAAACAGCTCAAAAATCTCCCTATGTGACAACCAAGGTGCCCCGATTTGTAGCTACTTGTACTCATGTCAGGCGATCAGCACAATCAACCTCCCGACGTCCACGTGTTGCGTTTACACGCCAGTGGATCTTGGGCCGGCTTTCGAGATGGATTTGGAGAAGCTGCAGTGCAGCTCTTACTCAGGGTTTTACAGCTTCAACGAACGTGAGTCTGATCCTAATAGTTGGAAGTACGGGATTGCACTCAAGTACAAGTTTAGTGTGAACAACGAGTATCCGAGTTCGTGTGCTAATTGTGAGAGGAGTAATGGGGTTTGCGGGTATTATGGGAATTACAACTCGTTTATATGTAATTGCCCCAGTGGGATCAACACCACTAATGATTGTTTCTTTGGAGCTTCATATAATTATGGTTCAAGGCCTCGATCGTGGCAGATTGGGACTTGGTTAATCTATTGCTTGGCTTGGTTATTGCTTTTGGTATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

307

Amino Acids

34.07

Weight (kDa)

5.17

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 91 3.2e-10 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 212 - 265 3.5e-08 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10380 AT1G10380
fragaria_vesca FvH4_4g35530
malus_domestica MD13G1082400.v1.1 MD16G1081500.v1.1
prunus_persica Prupe.3G121800_v2.0.a1 Prupe.3G121800_v2.0.a1
pyrus_communis pycom13g07100 pycom16g07030
rosa_chinensis RchiOBHm_Chr4g0445181
rosa_laevigata RLG00000005760
rosa_multiflora Rmu_sc0000050.1_g000037
rosa_roxburghii Rroxscaffold_5G00385690
rosa_rugosa Rorug04G0359700
rosa_samantha Rh4AG420500 Rh4BG432400 Rh4CG447000 Rh4DG428500
rosa_wichuraiana Rw4G036060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 470
AccII CGCG 2 cut(s) 158, 160
AciI CCGC 2 cut(s) 105, 750
AclI AACGTT 1 cut(s) 360
AclWI GGATC 6 cut(s) 93, 213, 226, 567, 641, 806
AcvI CACGTG 2 cut(s) 175, 537
AcyI GRCGYC 1 cut(s) 530
AfaI GTAC 4 cut(s) 263, 494, 665, 683
AfiI CCNNNNNNNGG 3 cut(s) 149, 327, 328
AflIII ACRYGT 3 cut(s) 174, 287, 536
AgsI TTSAA 4 cut(s) 21, 166, 631, 849
AjnI CCWGG 2 cut(s) 186, 348
AleI CACNNNNGTG 1 cut(s) 554
AluBI AGCT 7 cut(s) 12, 441, 486, 595, 606, 627, 830
AluI AGCT 7 cut(s) 12, 441, 486, 595, 606, 627, 830
AlwI GGATC 6 cut(s) 93, 213, 226, 567, 641, 806
AoxI GGCC 2 cut(s) 566, 851
ApeKI GCWGC 4 cut(s) 135, 183, 595, 603
ArsI GACNNNNNNTTYG 4 cut(s) 323, 347, 355, 379
AspLEI GCGC 2 cut(s) 160, 162
AspS9I GGNCC 1 cut(s) 566
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 2 cut(s) 31, 61
BaeGI GKGCMC 1 cut(s) 475
BamHI GGATCC 1 cut(s) 218
BanI GGYRCC 1 cut(s) 470
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
BbrPI CACGTG 2 cut(s) 175, 537
BbvI GCAGC 4 cut(s) 147, 195, 582, 615
BccI CCATC 5 cut(s) 44, 59, 206, 236, 574
BciT130I CCWGG 2 cut(s) 188, 350
BciVI GTATCC 1 cut(s) 717
BcnI CCSGG 1 cut(s) 144
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 596
BfuAI ACCTGC 1 cut(s) 98
BfuI GTATCC 1 cut(s) 717
BisI GCNGC 5 cut(s) 106, 136, 184, 596, 604
BlsI GCNGC 5 cut(s) 107, 137, 185, 597, 605
Bme1390I CCNGG 3 cut(s) 144, 188, 350
BmgT120I GGNCC 1 cut(s) 566
BmiI GGNNCC 2 cut(s) 220, 472
BmrFI CCNGG 3 cut(s) 144, 188, 350
BmrI ACTGGG 2 cut(s) 337, 786
BmuI ACTGGG 2 cut(s) 337, 786
BpuEI CTTGAG 2 cut(s) 104, 662
BpuMI CCSGG 1 cut(s) 144
BsaAI YACGTR 2 cut(s) 175, 537
BsaHI GRCGYC 1 cut(s) 530
BsaJI CCNNGG 3 cut(s) 142, 187, 466
BsaWI WCCGGW 1 cut(s) 215
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 327, 328
Bse118I RCCGGY 1 cut(s) 568
Bse1I ACTGG 3 cut(s) 332, 554, 792
BseBI CCWGG 2 cut(s) 188, 350
BseDI CCNNGG 3 cut(s) 142, 187, 466
BseLI CCNNNNNNNGG 3 cut(s) 149, 327, 328
BseMII CTCAG 2 cut(s) 282, 627
BseNI ACTGG 3 cut(s) 332, 554, 792
BsePI GCGCGC 1 cut(s) 158
BseRI GAGGAG 3 cut(s) 50, 70, 748
BseSI GKGCMC 1 cut(s) 475
BseXI GCAGC 4 cut(s) 147, 195, 582, 615
BsgI GTGCAG 2 cut(s) 202, 622
Bsh1236I CGCG 2 cut(s) 158, 160
Bsh1285I CGRYCG 1 cut(s) 860
BshFI GGCC 2 cut(s) 568, 853
BshNI GGYRCC 1 cut(s) 470
BsiEI CGRYCG 1 cut(s) 860
BsiSI CCGG 3 cut(s) 144, 216, 569
BslFI GGGAC 2 cut(s) 344, 886
BslI CCNNNNNNNGG 3 cut(s) 149, 327, 328
BsmFI GGGAC 2 cut(s) 344, 886
BsnI GGCC 2 cut(s) 568, 853
Bsp1286I GDGCHC 1 cut(s) 475
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 7 cut(s) 98, 218, 507, 559, 646, 798, 857
BspACI CCGC 2 cut(s) 105, 750
BspANI GGCC 2 cut(s) 568, 853
BspCNI CTCAG 2 cut(s) 281, 626
BspFNI CGCG 2 cut(s) 158, 160
BspLI GGNNCC 2 cut(s) 220, 472
BspMAI CTGCAG 1 cut(s) 600
BspMI ACCTGC 1 cut(s) 98
BspPI GGATC 6 cut(s) 93, 213, 226, 567, 641, 806
BspT107I GGYRCC 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 568
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 3 cut(s) 332, 554, 792
BssAI RCCGGY 1 cut(s) 568
BssECI CCNNGG 3 cut(s) 142, 187, 466
BssHII GCGCGC 1 cut(s) 158
BssMI GATC 7 cut(s) 98, 218, 507, 559, 646, 798, 857
BssNI GRCGYC 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 466
Bst2UI CCWGG 2 cut(s) 188, 350
BstACI GRCGYC 1 cut(s) 530
BstAUI TGTACA 1 cut(s) 261
BstBAI YACGTR 2 cut(s) 175, 537
BstC8I GCNNGC 2 cut(s) 160, 570
BstDEI CTNAG 2 cut(s) 268, 613
BstFNI CGCG 2 cut(s) 158, 160
BstHHI GCGC 2 cut(s) 160, 162
BstKTI GATC 7 cut(s) 101, 221, 510, 562, 649, 801, 860
BstMBI GATC 7 cut(s) 98, 218, 507, 559, 646, 798, 857
BstMCI CGRYCG 1 cut(s) 860
BstMWI GCNNNNNNNGC 2 cut(s) 144, 159
BstNI CCWGG 2 cut(s) 188, 350
BstNSI RCATGY 1 cut(s) 291
BstSCI CCNGG 3 cut(s) 142, 186, 348
BstSFI CTRYAG 1 cut(s) 596
BstSLI GKGCMC 1 cut(s) 475
BstUI CGCG 2 cut(s) 158, 160
BstV1I GCAGC 4 cut(s) 147, 195, 582, 615
BstX2I RGATCY 3 cut(s) 98, 218, 559
BstYI RGATCY 3 cut(s) 98, 218, 559
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 2 cut(s) 568, 853
BtsI GCAGTG 1 cut(s) 605
BtsIMutI CAGTG 4 cut(s) 423, 561, 605, 799
BveI ACCTGC 1 cut(s) 98
Cac8I GCNNGC 2 cut(s) 160, 570
CfoI GCGC 2 cut(s) 160, 162
Cfr10I RCCGGY 1 cut(s) 568
Cfr13I GGNCC 1 cut(s) 566
CseI GACGC 2 cut(s) 164, 341
Csp6I GTAC 4 cut(s) 262, 493, 664, 682
CviAII CATG 5 cut(s) 259, 280, 288, 294, 498
CviQI GTAC 4 cut(s) 262, 493, 664, 682
DdeI CTNAG 2 cut(s) 268, 613
DpnI GATC 7 cut(s) 100, 220, 509, 561, 648, 800, 859
DpnII GATC 7 cut(s) 98, 218, 507, 559, 646, 798, 857
Eco130I CCWWGG 1 cut(s) 466
Eco147I AGGCCT 1 cut(s) 853
Eco72I CACGTG 2 cut(s) 175, 537
EcoRII CCWGG 2 cut(s) 186, 348
EcoT14I CCWWGG 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 5 cut(s) 262, 283, 291, 297, 501
FaqI GGGAC 2 cut(s) 344, 886
FatI CATG 5 cut(s) 258, 279, 287, 293, 497
FauI CCCGC 1 cut(s) 743
Fnu4HI GCNGC 5 cut(s) 106, 136, 184, 596, 604
Fsp4HI GCNGC 5 cut(s) 106, 136, 184, 596, 604
FspBI CTAG 1 cut(s) 323
GlaI GCGC 2 cut(s) 159, 161
GluI GCNGC 5 cut(s) 106, 136, 184, 596, 604
HaeIII GGCC 2 cut(s) 568, 853
HapII CCGG 3 cut(s) 144, 216, 569
HgaI GACGC 2 cut(s) 164, 341
HhaI GCGC 2 cut(s) 160, 162
Hin1I GRCGYC 1 cut(s) 530
Hin1II CATG 5 cut(s) 262, 283, 291, 297, 501
Hin6I GCGC 2 cut(s) 158, 160
HinP1I GCGC 2 cut(s) 158, 160
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 1 cut(s) 641
HpaII CCGG 3 cut(s) 144, 216, 569
HphI GGTGA 2 cut(s) 31, 61
Hpy166II GTNNAC 4 cut(s) 285, 534, 547, 697
Hpy188I TCNGA 3 cut(s) 271, 646, 711
Hpy188III TCNNGA 3 cut(s) 232, 526, 577
Hpy8I GTNNAC 4 cut(s) 285, 534, 547, 697
Hpy99I CGWCG 2 cut(s) 158, 532
HpyAV CCTTC 5 cut(s) 27, 52, 82, 247, 349
HpyCH4IV ACGT 5 cut(s) 174, 360, 530, 536, 637
HpyCH4V TGCA 5 cut(s) 183, 297, 598, 603, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 159
HpyF3I CTNAG 2 cut(s) 268, 613
HpySE526I ACGT 5 cut(s) 174, 360, 530, 536, 637
Hsp92I GRCGYC 1 cut(s) 530
Hsp92II CATG 5 cut(s) 262, 283, 291, 297, 501
HspAI GCGC 2 cut(s) 158, 160
KroI GCCGGC 1 cut(s) 568
KroNI GCCGGC 1 cut(s) 570
Kzo9I GATC 7 cut(s) 98, 218, 507, 559, 646, 798, 857
LmnI GCTCC 1 cut(s) 827
Lsp1109I GCAGC 4 cut(s) 147, 195, 582, 615
MaeI CTAG 1 cut(s) 323
MaeII ACGT 5 cut(s) 174, 360, 530, 536, 637
MaeIII GTNAC 1 cut(s) 458
MalI GATC 7 cut(s) 100, 220, 509, 561, 648, 800, 859
MboI GATC 7 cut(s) 98, 218, 507, 559, 646, 798, 857
MboII GAAGA 3 cut(s) 46, 193, 394
MflI RGATCY 3 cut(s) 98, 218, 559
MhlI GDGCHC 1 cut(s) 475
MluCI AATT 4 cut(s) 724, 763, 784, 838
MlyI GAGTC 1 cut(s) 650
MmeI TCCRAC 3 cut(s) 88, 430, 638
MroNI GCCGGC 1 cut(s) 568
MseI TTAA 1 cut(s) 881
MslI CAYNNNNRTG 2 cut(s) 292, 554
MspI CCGG 3 cut(s) 144, 216, 569
MspR9I CCNGG 3 cut(s) 144, 188, 350
MvaI CCWGG 2 cut(s) 188, 350
MvnI CGCG 2 cut(s) 158, 160
MwoI GCNNNNNNNGC 2 cut(s) 144, 159
NaeI GCCGGC 1 cut(s) 570
NciI CCSGG 1 cut(s) 144
NdeII GATC 7 cut(s) 98, 218, 507, 559, 646, 798, 857
NgoMIV GCCGGC 1 cut(s) 568
NlaIII CATG 5 cut(s) 262, 283, 291, 297, 501
NlaIV GGNNCC 2 cut(s) 220, 472
NmuCI GTSAC 1 cut(s) 458
NspI RCATGY 1 cut(s) 291
OliI CACNNNNGTG 1 cut(s) 554
PauI GCGCGC 1 cut(s) 158
PceI AGGCCT 1 cut(s) 853
PciI ACATGT 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PdiI GCCGGC 1 cut(s) 570
PfoI TCCNGGA 1 cut(s) 348
PkrI GCNGC 5 cut(s) 107, 137, 185, 597, 605
Ple19I CGATCG 1 cut(s) 860
PleI GAGTC 1 cut(s) 649
PmaCI CACGTG 2 cut(s) 175, 537
PmlI CACGTG 2 cut(s) 175, 537
PpsI GAGTC 1 cut(s) 649
Ppu21I YACGTR 2 cut(s) 175, 537
PscI ACATGT 1 cut(s) 287
Psp1406I AACGTT 1 cut(s) 360
Psp6I CCWGG 2 cut(s) 186, 348
PspCI CACGTG 2 cut(s) 175, 537
PspGI CCWGG 2 cut(s) 186, 348
PspN4I GGNNCC 2 cut(s) 220, 472
PspPI GGNCC 1 cut(s) 566
PsrI GAACNNNNNNTAC 2 cut(s) 227, 259
PstI CTGCAG 1 cut(s) 600
PsuI RGATCY 3 cut(s) 98, 218, 559
PteI GCGCGC 1 cut(s) 158
PvuI CGATCG 1 cut(s) 860
RsaI GTAC 4 cut(s) 263, 494, 665, 683
RsaNI GTAC 4 cut(s) 262, 493, 664, 682
RseI CAYNNNNRTG 2 cut(s) 292, 554
SaqAI TTAA 1 cut(s) 881
SatI GCNGC 5 cut(s) 106, 136, 184, 596, 604
Sau3AI GATC 7 cut(s) 98, 218, 507, 559, 646, 798, 857
Sau96I GGNCC 1 cut(s) 566
SchI GAGTC 1 cut(s) 650
ScrFI CCNGG 3 cut(s) 144, 188, 350
SduI GDGCHC 1 cut(s) 475
SfcI CTRYAG 1 cut(s) 596
SmiMI CAYNNNNRTG 2 cut(s) 292, 554
SmlI CTYRAG 2 cut(s) 119, 677
SmoI CTYRAG 2 cut(s) 119, 677
Sse9I AATT 4 cut(s) 724, 763, 784, 838
SseBI AGGCCT 1 cut(s) 853
SsiI CCGC 2 cut(s) 105, 750
SspMI CTAG 1 cut(s) 323
StuI AGGCCT 1 cut(s) 853
StyD4I CCNGG 3 cut(s) 142, 186, 348
StyI CCWWGG 1 cut(s) 466
TaiI ACGT 5 cut(s) 177, 363, 533, 539, 640
TaqI TCGA 4 cut(s) 342, 375, 576, 856
TasI AATT 4 cut(s) 724, 763, 784, 838
TatI WGTACW 3 cut(s) 261, 492, 681
TauI GCSGC 1 cut(s) 108
Tru1I TTAA 1 cut(s) 881
Tru9I TTAA 1 cut(s) 881
TscAI CASTG 4 cut(s) 430, 561, 605, 799
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 4 cut(s) 135, 183, 595, 603
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 4 cut(s) 17, 64, 282, 822
TspRI CASTG 4 cut(s) 430, 561, 605, 799
XceI RCATGY 1 cut(s) 291
XspI CTAG 1 cut(s) 323
ZraI GACGTC 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.