Rroxscaffold_5G00385690

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012438
Physical Location & Seq
Reverse (-)
64734591 .. 64736378
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00385690.1

Sequence Viewer

Length: 1008 bp
ATGAAGCTTAGTCATCCTTCAACTACTATCACCTCCTTCATCTTCTTCATCTTCCTTACTCTACCCTCCTCAATCACATCCCAAATTTGCCAAAGATCCTGCAACAGAATCCCTCTTAACTTCCCATTCGGCAGTGACGCCGGCTGCGGCGACCCGCGCTTCCACCAATCCGTAACATGCAACCAAGACAAGCTCACCTTCACCACCCACACCGGATCCTACCCCATCACCAACATAGACTACACAAAACAAGTCCTCTACATCTCAGACCCCTCCATGTCCACCTGCTCCTGCACTCAACCCAGCAAAGGCTTCAGCCTCAACTACGACGCGCCGTTCACCTTCCAAGACGACAACATCTTCGCCTTGCTCGACTGCTCCACCGCATCATCCAACGGCAGCCTTTACAGCAGTGACAACTCCAAGCTCTCTCTTTGTGATAACCAAGGTGCACCCATCTGCAGCTACTTGTATTCATGCAGGGCTATAAGCTTCATCAATCTCCCCATCGCCACGTGTTGCGTTTACACTCCGGTGGATCTCGGGCCGGCTTTCGAGATGGACTTGCAGAAGCTACAATGCAGCTCTTATTCTGGTTTTTACAGCTTCAATGGCCGTGAATCCGACCCTAATAGTTGGAAGTATGGGATAGCGCTCAAGTATAAGTTCAACGTGAATAATGAGTTTCCCAGTTCATGTTTTAATTGTGAGAGGAGTAATGGGGTTTGTGGGTACTATGGAGCTTATAACTCGTTTATATGTAATTGTCCGAATGGTTTGAACACTACAAACGATTGCTTCTTTGGATCGCCCTATAGTTATGGCTCGAGGCTTATTGCATCGTGGCACTCTGTCGCTAAGATACAAGTACTGAATCCTAAACCAGAAAACGAGAAGGACCAAAACCTCTTATGTTTAGGATCAGCTGATCATAGGAGAGAAGATGGGCAAAATGAAGGGATTGGAAAAGGGCTCAGCAGCTTCGTAAATAACTTAAACCCCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

335

Amino Acids

37.02

Weight (kDa)

5.71

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 30 - 90 6.8e-10 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 207 - 258 3.4e-07 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10380 AT1G10380
fragaria_vesca FvH4_4g35530
malus_domestica MD13G1082400.v1.1 MD16G1081500.v1.1
prunus_persica Prupe.3G121800_v2.0.a1 Prupe.3G121800_v2.0.a1
pyrus_communis pycom13g07100 pycom16g07030
rosa_chinensis RchiOBHm_Chr4g0445181
rosa_laevigata RLG00000005760
rosa_multiflora Rmu_sc0000050.1_g000037
rosa_roxburghii Rroxscaffold_5G00385690
rosa_rugosa Rorug04G0359700
rosa_samantha Rh4AG420500 Rh4BG432400 Rh4CG447000 Rh4DG428500
rosa_wichuraiana Rw4G036060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AarI CACCTGC 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 293
AccII CGCG 2 cut(s) 157, 332
AciI CCGC 3 cut(s) 147, 155, 384
AclWI GGATC 6 cut(s) 90, 210, 223, 546, 814, 928
AcoI YGGCCR 1 cut(s) 613
AcsI RAATTY 1 cut(s) 84
AcuI CTGAAG 1 cut(s) 298
AcvI CACGTG 1 cut(s) 516
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 2 cut(s) 734, 870
AfeI AGCGCT 1 cut(s) 654
AfiI CCNNNNNNNGG 1 cut(s) 308
AflIII ACRYGT 1 cut(s) 515
AgsI TTSAA 4 cut(s) 21, 610, 670, 781
AleI CACNNNNGTG 1 cut(s) 533
Alw21I GWGCWC 1 cut(s) 454
Alw44I GTGCAC 1 cut(s) 450
AlwI GGATC 6 cut(s) 90, 210, 223, 546, 814, 928
Ama87I CYCGRG 2 cut(s) 542, 826
Aor51HI AGCGCT 1 cut(s) 654
AoxI GGCC 2 cut(s) 545, 613
ApaLI GTGCAC 1 cut(s) 450
ApeKI GCWGC 5 cut(s) 144, 399, 462, 582, 978
ApoI RAATTY 1 cut(s) 84
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AspLEI GCGC 3 cut(s) 159, 334, 655
AspS9I GGNCC 2 cut(s) 545, 898
AsuHPI GGTGA 5 cut(s) 22, 187, 193, 220, 331
AvaI CYCGRG 2 cut(s) 542, 826
AvaII GGWCC 1 cut(s) 898
BaeGI GKGCMC 1 cut(s) 454
BamHI GGATCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 975
BbrPI CACGTG 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 5 cut(s) 131, 411, 474, 594, 990
BccI CCATC 5 cut(s) 233, 464, 515, 553, 938
BceAI ACGGC 3 cut(s) 319, 412, 600
BclI TGATCA 1 cut(s) 928
BfmI CTRYAG 2 cut(s) 460, 814
BfoI RGCGCY 1 cut(s) 656
BfuAI ACCTGC 1 cut(s) 293
BisI GCNGC 6 cut(s) 145, 148, 400, 463, 583, 979
BlpI GCTNAGC 1 cut(s) 974
BlsI GCNGC 6 cut(s) 146, 149, 401, 464, 584, 980
BmcAI AGTACT 1 cut(s) 870
Bme18I GGWCC 1 cut(s) 898
BmeT110I CYCGRG 2 cut(s) 542, 826
BmgT120I GGNCC 2 cut(s) 545, 898
BmiI GGNNCC 1 cut(s) 217
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 2 cut(s) 395, 848
BmuI ACTGGG 1 cut(s) 684
Bpu1102I GCTNAGC 1 cut(s) 974
BpuEI CTTGAG 1 cut(s) 641
BsaAI YACGTR 1 cut(s) 516
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 445
BsaWI WCCGGW 2 cut(s) 212, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse118I RCCGGY 2 cut(s) 140, 547
Bse1I ACTGG 1 cut(s) 690
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 3 cut(s) 13, 77, 389
BseLI CCNNNNNNNGG 1 cut(s) 308
BseMII CTCAG 2 cut(s) 279, 988
BseNI ACTGG 1 cut(s) 690
BseRI GAGGAG 2 cut(s) 58, 727
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 5 cut(s) 131, 411, 474, 594, 990
BseYI CCCAGC 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 277
Bsh1236I CGCG 2 cut(s) 157, 332
BshFI GGCC 2 cut(s) 547, 615
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 2 cut(s) 542, 826
BsiSI CCGG 4 cut(s) 141, 213, 533, 548
BslI CCNNNNNNNGG 1 cut(s) 308
BsnI GGCC 2 cut(s) 547, 615
BsoBI CYCGRG 2 cut(s) 542, 826
Bsp1286I GDGCHC 2 cut(s) 454, 975
Bsp143I GATC 6 cut(s) 95, 215, 538, 806, 920, 928
Bsp1720I GCTNAGC 1 cut(s) 974
BspACI CCGC 3 cut(s) 147, 155, 384
BspANI GGCC 2 cut(s) 547, 615
BspCNI CTCAG 2 cut(s) 278, 987
BspFNI CGCG 2 cut(s) 157, 332
BspLI GGNNCC 1 cut(s) 217
BspMAI CTGCAG 1 cut(s) 464
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 6 cut(s) 90, 210, 223, 546, 814, 928
BsrFI RCCGGY 2 cut(s) 140, 547
BsrI ACTGG 1 cut(s) 690
BssAI RCCGGY 2 cut(s) 140, 547
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 6 cut(s) 95, 215, 538, 806, 920, 928
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 445
BstACI GRCGYC 1 cut(s) 138
BstBAI YACGTR 1 cut(s) 516
BstC8I GCNNGC 2 cut(s) 142, 549
BstDEI CTNAG 4 cut(s) 8, 265, 858, 974
BstF5I GGATG 3 cut(s) 13, 77, 389
BstFNI CGCG 2 cut(s) 157, 332
BstH2I RGCGCY 1 cut(s) 656
BstHHI GCGC 3 cut(s) 159, 334, 655
BstKTI GATC 6 cut(s) 98, 218, 541, 809, 923, 931
BstMBI GATC 6 cut(s) 95, 215, 538, 806, 920, 928
BstMWI GCNNNNNNNGC 3 cut(s) 156, 408, 612
BstNSI RCATGY 1 cut(s) 180
BstSFI CTRYAG 2 cut(s) 460, 814
BstSLI GKGCMC 1 cut(s) 454
BstUI CGCG 2 cut(s) 157, 332
BstV1I GCAGC 5 cut(s) 131, 411, 474, 594, 990
BstX2I RGATCY 3 cut(s) 95, 215, 538
BstYI RGATCY 3 cut(s) 95, 215, 538
BsuRI GGCC 2 cut(s) 547, 615
BtgZI GCGATG 1 cut(s) 493
BtsCI GGATG 3 cut(s) 13, 77, 389
BtsI GCAGTG 2 cut(s) 139, 418
BtsIMutI CAGTG 2 cut(s) 139, 418
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 2 cut(s) 142, 549
CfoI GCGC 3 cut(s) 159, 334, 655
Cfr10I RCCGGY 2 cut(s) 140, 547
Cfr13I GGNCC 2 cut(s) 545, 898
CseI GACGC 2 cut(s) 146, 338
Csp6I GTAC 2 cut(s) 733, 869
CviAII CATG 4 cut(s) 177, 277, 477, 696
CviQI GTAC 2 cut(s) 733, 869
DdeI CTNAG 4 cut(s) 8, 265, 858, 974
DpnI GATC 6 cut(s) 97, 217, 540, 808, 922, 930
DpnII GATC 6 cut(s) 95, 215, 538, 806, 920, 928
EaeI YGGCCR 1 cut(s) 613
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 975
Eco47I GGWCC 1 cut(s) 898
Eco47III AGCGCT 1 cut(s) 654
Eco57I CTGAAG 1 cut(s) 298
Eco72I CACGTG 1 cut(s) 516
Eco88I CYCGRG 2 cut(s) 542, 826
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 975
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 4 cut(s) 180, 280, 480, 699
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FatI CATG 4 cut(s) 176, 276, 476, 695
FauI CCCGC 1 cut(s) 162
FbaI TGATCA 1 cut(s) 928
Fnu4HI GCNGC 6 cut(s) 145, 148, 400, 463, 583, 979
FokI GGATG 2 cut(s) 64, 376
FriOI GRGCYC 1 cut(s) 975
Fsp4HI GCNGC 6 cut(s) 145, 148, 400, 463, 583, 979
GlaI GCGC 3 cut(s) 158, 333, 654
GluI GCNGC 6 cut(s) 145, 148, 400, 463, 583, 979
GsaI CCCAGC 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 656
HaeIII GGCC 2 cut(s) 547, 615
HapII CCGG 4 cut(s) 141, 213, 533, 548
HgaI GACGC 2 cut(s) 146, 338
HhaI GCGC 3 cut(s) 159, 334, 655
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 4 cut(s) 180, 280, 480, 699
Hin6I GCGC 3 cut(s) 157, 332, 653
HinP1I GCGC 3 cut(s) 157, 332, 653
HindIII AAGCTT 2 cut(s) 5, 490
HinfI GANTC 3 cut(s) 108, 620, 874
HpaII CCGG 4 cut(s) 141, 213, 533, 548
HphI GGTGA 5 cut(s) 22, 187, 193, 220, 331
Hpy166II GTNNAC 4 cut(s) 282, 339, 452, 526
Hpy188I TCNGA 3 cut(s) 268, 625, 771
Hpy188III TCNNGA 1 cut(s) 556
Hpy8I GTNNAC 4 cut(s) 282, 339, 452, 526
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 6 cut(s) 27, 46, 208, 352, 889, 950
HpyCH4IV ACGT 2 cut(s) 515, 672
HpyCH4V TGCA 9 cut(s) 102, 180, 294, 452, 462, 480, 568, 582, 839
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 408, 612
HpyF3I CTNAG 4 cut(s) 8, 265, 858, 974
HpySE526I ACGT 2 cut(s) 515, 672
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 4 cut(s) 180, 280, 480, 699
HspAI GCGC 3 cut(s) 157, 332, 653
KroI GCCGGC 2 cut(s) 140, 547
KroNI GCCGGC 2 cut(s) 142, 549
Ksp22I TGATCA 1 cut(s) 928
Kzo9I GATC 6 cut(s) 95, 215, 538, 806, 920, 928
LmnI GCTCC 3 cut(s) 293, 383, 740
Lsp1109I GCAGC 5 cut(s) 131, 411, 474, 594, 990
LweI GCATC 2 cut(s) 395, 848
MaeII ACGT 2 cut(s) 515, 672
MaeIII GTNAC 3 cut(s) 134, 172, 413
MalI GATC 6 cut(s) 97, 217, 540, 808, 922, 930
MboI GATC 6 cut(s) 95, 215, 538, 806, 920, 928
MboII GAAGA 5 cut(s) 34, 37, 43, 352, 953
MflI RGATCY 3 cut(s) 95, 215, 538
MhlI GDGCHC 2 cut(s) 454, 975
MluCI AATT 3 cut(s) 84, 703, 763
MmeI TCCRAC 3 cut(s) 417, 617, 648
MroNI GCCGGC 2 cut(s) 140, 547
MseI TTAA 3 cut(s) 117, 702, 995
MslI CAYNNNNRTG 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 4 cut(s) 141, 213, 533, 548
MvnI CGCG 2 cut(s) 157, 332
MwoI GCNNNNNNNGC 3 cut(s) 156, 408, 612
NaeI GCCGGC 2 cut(s) 142, 549
NdeII GATC 6 cut(s) 95, 215, 538, 806, 920, 928
NgoMIV GCCGGC 2 cut(s) 140, 547
NlaIII CATG 4 cut(s) 180, 280, 480, 699
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 2 cut(s) 134, 413
NspI RCATGY 1 cut(s) 180
OliI CACNNNNGTG 1 cut(s) 533
PaeR7I CTCGAG 1 cut(s) 826
PaqCI CACCTGC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PdiI GCCGGC 2 cut(s) 142, 549
PfeI GAWTC 3 cut(s) 108, 620, 874
PkrI GCNGC 6 cut(s) 146, 149, 401, 464, 584, 980
PmaCI CACGTG 1 cut(s) 516
PmlI CACGTG 1 cut(s) 516
Ppu21I YACGTR 1 cut(s) 516
PsiI TTATAA 1 cut(s) 747
PspCI CACGTG 1 cut(s) 516
PspFI CCCAGC 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 2 cut(s) 545, 898
PspXI VCTCGAGB 1 cut(s) 826
PstI CTGCAG 1 cut(s) 464
PsuI RGATCY 3 cut(s) 95, 215, 538
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 2 cut(s) 734, 870
RsaNI GTAC 2 cut(s) 733, 869
RseI CAYNNNNRTG 1 cut(s) 533
SaqAI TTAA 3 cut(s) 117, 702, 995
SatI GCNGC 6 cut(s) 145, 148, 400, 463, 583, 979
Sau3AI GATC 6 cut(s) 95, 215, 538, 806, 920, 928
Sau96I GGNCC 2 cut(s) 545, 898
ScaI AGTACT 1 cut(s) 870
SduI GDGCHC 2 cut(s) 454, 975
SfaNI GCATC 2 cut(s) 395, 848
SfcI CTRYAG 2 cut(s) 460, 814
Sfr274I CTCGAG 1 cut(s) 826
SinI GGWCC 1 cut(s) 898
SlaI CTCGAG 1 cut(s) 826
SmiMI CAYNNNNRTG 1 cut(s) 533
SmlI CTYRAG 2 cut(s) 656, 826
SmoI CTYRAG 2 cut(s) 656, 826
Sse9I AATT 3 cut(s) 84, 703, 763
SsiI CCGC 3 cut(s) 147, 155, 384
StyI CCWWGG 1 cut(s) 445
TaiI ACGT 2 cut(s) 518, 675
TaqI TCGA 3 cut(s) 372, 555, 827
TasI AATT 3 cut(s) 84, 703, 763
TatI WGTACW 1 cut(s) 868
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 3 cut(s) 108, 620, 874
Tru1I TTAA 3 cut(s) 117, 702, 995
Tru9I TTAA 3 cut(s) 117, 702, 995
TscAI CASTG 2 cut(s) 139, 418
TseFI GTSAC 2 cut(s) 134, 413
TseI GCWGC 5 cut(s) 144, 399, 462, 582, 978
Tsp45I GTSAC 2 cut(s) 134, 413
TspDTI ATGAA 7 cut(s) 17, 28, 37, 465, 484, 684, 969
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 2 cut(s) 139, 418
VneI GTGCAC 1 cut(s) 450
VpaK11BI GGWCC 1 cut(s) 898
XapI RAATTY 1 cut(s) 84
XceI RCATGY 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 826
ZrmI AGTACT 1 cut(s) 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.