FvH4_4g36680

UPF0496 protein 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014732
Physical Location & Seq
Reverse (-)
33495727 .. 33496677
951 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36680.t1

Sequence Viewer

Length: 951 bp
ATGAACAAACACGCCGTTCCTTCCTTCAAACACTCTCTTCCTAATAAACTCGACCTCATCACGGCCGCCTTCGACGACGCTCTCCTCCGTAGTCTCAAGACGCTTTCTGCGCCGTCGATCAGCTTGTGCTGGCTGTCTAAGGCCGTCGAATTTCTCGCCGTCGCACACGCCGAGGCCAGGAGGCTAATCTTCTCCAGTCTCGAGGTAACCGTTTTGGATGACGCTCTTGCATCTTATTTGGATGACAGCGTGAAGCTCCTGGACATATGCAACTCCGTCTCCGCCGAGATCGAGCGGCTCCGCCAGCGCCGGATTTTTCTCGCTCTCGCGGTTCACCTCCTCGGCGACGGGAAGGTCCGGCGAGCGCGGGAGGCTTTAACGGAAGGGGAGGAGGCCCGCCGCGCTGCTTCGGGATTTGGGAAAGCGAATAATGCGGAGGCGCTGGTGAAGGATCTGGCGCTGGCGCTCCTCCGCAGCGCGCCGCCACGTGGAAAGATCTCAAAGGTCGCGAGAGTCGTGCGTCGGACGGTGTACGCCGTCGGATTGGTGACCGTGTTCGTCGCCGGAGCATTAGTCTCGGCGATGGAGGGAACGCCGGAGACGGTGACGGTCCGGGCGCCGGCGGAGTATTCTTGGGCCGAGTCGTTGAATGAGCTGGGAACGGAGGTCTCCGGCGAGCTGAAACGGCGATTCGGAAAGGAGGAGAGAACGAAAGGTCTGCTGTTGGAGGAGGTGGATGACGTGGCTACACGCGTCGGGGAAGCGTGTGAGATGGCGGAAGAGGACACGGAGAGGAAAGAGCGGTTGAACGACGTCGTAAAAGAGGTGGAGAGGGCGATGGGGTCGTTTTCTGATGGGTTGGAAGGGTTGAGTAAAGGAATGAATGTAATGTTTCATGGTGTTTTGAGTAACAGGAACTTCATGTTGAACCATGTTAGGGTCAAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

317

Amino Acids

34.6

Weight (kDa)

7.04

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 30 - 128 5.5e-12 Protein ROH1-like
ROH1-like PF05633 160 - 310 1.7e-08 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36680
malus_domestica MD13G1008300.v1.1
prunus_persica Prupe.1G344800_v2.0.a1
pyrus_communis pycom13g00680
rosa_chinensis RchiOBHm_Chr4g0446391
rosa_laevigata RLG00000005666
rosa_multiflora Rmu_sc0008360.1_g000003 Rmu_sc0010778.1_g000005 Rmu_sc0025512.1_g000002 Rmu_sc0025513.1_g000001
rosa_roxburghii Rroxscaffold_5G00386830
rosa_rugosa Rorug04G0371000
rosa_samantha Rh4AG432500 Rh4BG441700 Rh4CG459000 Rh4DG440400
rosa_wichuraiana Rw4G036940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
AatII GACGTC 1 cut(s) 816
AccB1I GGYRCC 1 cut(s) 616
AccBSI CCGCTC 2 cut(s) 295, 802
AccII CGCG 6 cut(s) 329, 367, 402, 479, 509, 753
AclWI GGATC 1 cut(s) 459
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 149
AcvI CACGTG 1 cut(s) 488
AcyI GRCGYC 2 cut(s) 617, 813
AfaI GTAC 1 cut(s) 533
AfiI CCNNNNNNNGG 5 cut(s) 61, 177, 488, 619, 937
AflIII ACRYGT 1 cut(s) 751
AgsI TTSAA 4 cut(s) 28, 649, 808, 928
AjiI CACGTC 1 cut(s) 742
AjnI CCWGG 2 cut(s) 176, 258
AluBI AGCT 5 cut(s) 123, 256, 655, 679, 947
AluI AGCT 5 cut(s) 123, 256, 655, 679, 947
Alw26I GTCTC 6 cut(s) 98, 203, 283, 580, 593, 673
AlwI GGATC 1 cut(s) 459
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 5 cut(s) 63, 141, 174, 393, 636
ApeKI GCWGC 2 cut(s) 404, 474
ApoI RAATTY 1 cut(s) 149
AspS9I GGNCC 4 cut(s) 355, 394, 610, 636
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 4 cut(s) 326, 457, 559, 616
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 2 cut(s) 355, 610
BanI GGYRCC 1 cut(s) 616
BbrPI CACGTG 1 cut(s) 488
BbvI GCAGC 2 cut(s) 391, 486
BccI CCATC 4 cut(s) 577, 766, 832, 848
BceAI ACGGC 6 cut(s) 78, 97, 128, 143, 521, 701
BcgI CGANNNNNNTGC 6 cut(s) 499, 533, 558, 592, 700, 734
BciT130I CCWGG 2 cut(s) 178, 260
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 6 cut(s) 98, 203, 283, 580, 593, 673
BfoI RGCGCY 5 cut(s) 310, 443, 461, 467, 620
BglII AGATCT 1 cut(s) 495
BisI GCNGC 6 cut(s) 66, 296, 400, 405, 475, 482
BlsI GCNGC 6 cut(s) 67, 297, 401, 406, 476, 483
Bme1390I CCNGG 3 cut(s) 178, 260, 614
Bme18I GGWCC 2 cut(s) 355, 610
BmeT110I CYCGRG 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 742
BmgT120I GGNCC 4 cut(s) 355, 394, 610, 636
BmiI GGNNCC 2 cut(s) 299, 618
BmrFI CCNGG 3 cut(s) 178, 260, 614
BmsI GCATC 1 cut(s) 239
BpmI CTGGAG 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 614
BsaAI YACGTR 1 cut(s) 488
BsaHI GRCGYC 2 cut(s) 617, 813
BsaI GGTCTC 1 cut(s) 673
BsaJI CCNNGG 2 cut(s) 171, 340
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 558, 588
Bsc4I CCNNNNNNNGG 5 cut(s) 61, 177, 488, 619, 937
Bse118I RCCGGY 1 cut(s) 619
Bse1I ACTGG 1 cut(s) 195
BseBI CCWGG 2 cut(s) 178, 260
BseDI CCNNGG 2 cut(s) 171, 340
BseGI GGATG 3 cut(s) 223, 247, 742
BseLI CCNNNNNNNGG 5 cut(s) 61, 177, 488, 619, 937
BseNI ACTGG 1 cut(s) 195
BsePI GCGCGC 1 cut(s) 477
BseRI GAGGAG 6 cut(s) 74, 329, 404, 458, 716, 743
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 2 cut(s) 391, 486
BseYI CCCAGC 1 cut(s) 655
Bsh1236I CGCG 6 cut(s) 329, 367, 402, 479, 509, 753
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 5 cut(s) 65, 143, 176, 395, 638
BshNI GGYRCC 1 cut(s) 616
BsiEI CGRYCG 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 7 cut(s) 310, 358, 564, 596, 613, 620, 672
BslI CCNNNNNNNGG 5 cut(s) 61, 177, 488, 619, 937
BsmAI GTCTC 6 cut(s) 98, 203, 283, 580, 593, 673
BsmBI CGTCTC 2 cut(s) 283, 593
BsnI GGCC 5 cut(s) 65, 143, 176, 395, 638
Bso31I GGTCTC 1 cut(s) 673
BsoBI CYCGRG 1 cut(s) 200
Bsp143I GATC 4 cut(s) 117, 288, 451, 495
Bsp68I TCGCGA 1 cut(s) 509
BspANI GGCC 5 cut(s) 65, 143, 176, 395, 638
BspFNI CGCG 6 cut(s) 329, 367, 402, 479, 509, 753
BspLI GGNNCC 2 cut(s) 299, 618
BspPI GGATC 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 616
BspTNI GGTCTC 1 cut(s) 673
BsrBI CCGCTC 2 cut(s) 295, 802
BsrFI RCCGGY 1 cut(s) 619
BsrI ACTGG 1 cut(s) 195
BssAI RCCGGY 1 cut(s) 619
BssECI CCNNGG 2 cut(s) 171, 340
BssHII GCGCGC 1 cut(s) 477
BssMI GATC 4 cut(s) 117, 288, 451, 495
BssNI GRCGYC 2 cut(s) 617, 813
Bst2UI CCWGG 2 cut(s) 178, 260
Bst4CI ACNGT 5 cut(s) 211, 529, 553, 604, 610
Bst6I CTCTTC 2 cut(s) 42, 774
BstACI GRCGYC 2 cut(s) 617, 813
BstBAI YACGTR 1 cut(s) 488
BstC8I GCNNGC 8 cut(s) 131, 305, 363, 397, 462, 479, 621, 677
BstDEI CTNAG 2 cut(s) 138, 948
BstEII GGTNACC 2 cut(s) 205, 547
BstF5I GGATG 3 cut(s) 223, 247, 742
BstFNI CGCG 6 cut(s) 329, 367, 402, 479, 509, 753
BstH2I RGCGCY 5 cut(s) 310, 443, 461, 467, 620
BstKTI GATC 4 cut(s) 120, 291, 454, 498
BstMAI GTCTC 6 cut(s) 98, 203, 283, 580, 593, 673
BstMBI GATC 4 cut(s) 117, 288, 451, 495
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 6 cut(s) 109, 304, 371, 401, 431, 685
BstNI CCWGG 2 cut(s) 178, 260
BstPI GGTNACC 2 cut(s) 205, 547
BstSCI CCNGG 3 cut(s) 176, 258, 612
BstUI CGCG 6 cut(s) 329, 367, 402, 479, 509, 753
BstV1I GCAGC 2 cut(s) 391, 486
BstX2I RGATCY 2 cut(s) 451, 495
BstYI RGATCY 2 cut(s) 451, 495
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 5 cut(s) 65, 143, 176, 395, 638
BtgZI GCGATG 2 cut(s) 596, 851
BtrI CACGTC 1 cut(s) 742
BtsCI GGATG 3 cut(s) 223, 247, 742
BtuMI TCGCGA 1 cut(s) 509
Cac8I GCNNGC 8 cut(s) 131, 305, 363, 397, 462, 479, 621, 677
Cfr10I RCCGGY 1 cut(s) 619
Cfr13I GGNCC 4 cut(s) 355, 394, 610, 636
CpoI CGGWCCG 1 cut(s) 610
CseI GACGC 5 cut(s) 86, 109, 230, 509, 742
Csp6I GTAC 1 cut(s) 532
CspI CGGWCCG 1 cut(s) 610
CviAII CATG 3 cut(s) 896, 922, 932
CviQI GTAC 1 cut(s) 532
DdeI CTNAG 2 cut(s) 138, 948
DinI GGCGCC 1 cut(s) 618
DpnI GATC 4 cut(s) 119, 290, 453, 497
DpnII GATC 4 cut(s) 117, 288, 451, 495
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 63
EagI CGGCCG 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 42, 774
EarI CTCTTC 2 cut(s) 42, 774
EciI GGCGGA 4 cut(s) 271, 290, 638, 791
EclXI CGGCCG 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 673
Eco47I GGWCC 2 cut(s) 355, 610
Eco52I CGGCCG 1 cut(s) 63
Eco72I CACGTG 1 cut(s) 488
Eco88I CYCGRG 1 cut(s) 200
Eco91I GGTNACC 2 cut(s) 205, 547
EcoO65I GGTNACC 2 cut(s) 205, 547
EcoRII CCWGG 2 cut(s) 176, 258
EgeI GGCGCC 1 cut(s) 618
EheI GGCGCC 1 cut(s) 618
Esp3I CGTCTC 2 cut(s) 283, 593
FaeI CATG 3 cut(s) 899, 925, 935
FaiI YATR 5 cut(s) 266, 268, 897, 923, 933
FatI CATG 3 cut(s) 895, 921, 931
FauI CCCGC 2 cut(s) 360, 404
FauNDI CATATG 1 cut(s) 266
Fnu4HI GCNGC 6 cut(s) 66, 296, 400, 405, 475, 482
FokI GGATG 3 cut(s) 230, 254, 749
Fsp4HI GCNGC 6 cut(s) 66, 296, 400, 405, 475, 482
GluI GCNGC 6 cut(s) 66, 296, 400, 405, 475, 482
GsaI CCCAGC 1 cut(s) 659
GsuI CTGGAG 1 cut(s) 178
HaeII RGCGCY 5 cut(s) 310, 443, 461, 467, 620
HaeIII GGCC 5 cut(s) 65, 143, 176, 395, 638
HapII CCGG 7 cut(s) 310, 358, 564, 596, 613, 620, 672
HgaI GACGC 5 cut(s) 86, 109, 230, 509, 742
Hin1I GRCGYC 2 cut(s) 617, 813
Hin1II CATG 3 cut(s) 899, 925, 935
HindIII AAGCTT 1 cut(s) 945
HinfI GANTC 3 cut(s) 513, 641, 690
HpaII CCGG 7 cut(s) 310, 358, 564, 596, 613, 620, 672
HphI GGTGA 4 cut(s) 326, 457, 559, 616
Hpy166II GTNNAC 2 cut(s) 334, 532
Hpy188I TCNGA 4 cut(s) 525, 542, 695, 853
Hpy188III TCNNGA 4 cut(s) 97, 200, 411, 508
Hpy8I GTNNAC 2 cut(s) 334, 532
HpyAV CCTTC 7 cut(s) 30, 34, 79, 346, 377, 442, 857
HpyCH4III ACNGT 5 cut(s) 211, 529, 553, 604, 610
HpyCH4IV ACGT 3 cut(s) 487, 741, 813
HpyCH4V TGCA 2 cut(s) 230, 270
HpyF10VI GCNNNNNNNGC 6 cut(s) 109, 304, 371, 401, 431, 685
HpyF3I CTNAG 2 cut(s) 138, 948
HpySE526I ACGT 3 cut(s) 487, 741, 813
Hsp92I GRCGYC 2 cut(s) 617, 813
Hsp92II CATG 3 cut(s) 899, 925, 935
KasI GGCGCC 1 cut(s) 616
KroI GCCGGC 1 cut(s) 619
KroNI GCCGGC 1 cut(s) 621
Kzo9I GATC 4 cut(s) 117, 288, 451, 495
LmnI GCTCC 4 cut(s) 261, 303, 471, 566
Lsp1109I GCAGC 2 cut(s) 391, 486
LweI GCATC 1 cut(s) 239
MaeII ACGT 3 cut(s) 487, 741, 813
MaeIII GTNAC 4 cut(s) 205, 547, 604, 908
MalI GATC 4 cut(s) 119, 290, 453, 497
MbiI CCGCTC 2 cut(s) 295, 802
MboI GATC 4 cut(s) 117, 288, 451, 495
MboII GAAGA 3 cut(s) 29, 181, 791
MflI RGATCY 2 cut(s) 451, 495
MluCI AATT 1 cut(s) 149
MluI ACGCGT 1 cut(s) 751
Mly113I GGCGCC 1 cut(s) 617
MlyI GAGTC 2 cut(s) 522, 650
MmeI TCCRAC 4 cut(s) 503, 520, 705, 840
MreI CGCCGGCG 1 cut(s) 619
MroNI GCCGGC 1 cut(s) 619
MseI TTAA 1 cut(s) 377
MspI CCGG 7 cut(s) 310, 358, 564, 596, 613, 620, 672
MspR9I CCNGG 3 cut(s) 178, 260, 614
MvaI CCWGG 2 cut(s) 178, 260
MvnI CGCG 6 cut(s) 329, 367, 402, 479, 509, 753
MwoI GCNNNNNNNGC 6 cut(s) 109, 304, 371, 401, 431, 685
NaeI GCCGGC 1 cut(s) 621
NarI GGCGCC 1 cut(s) 617
NciI CCSGG 1 cut(s) 614
NdeI CATATG 1 cut(s) 266
NdeII GATC 4 cut(s) 117, 288, 451, 495
NgoMIV GCCGGC 1 cut(s) 619
NlaIII CATG 3 cut(s) 899, 925, 935
NlaIV GGNNCC 2 cut(s) 299, 618
NmeAIII GCCGAG 5 cut(s) 196, 310, 321, 557, 664
NmuCI GTSAC 2 cut(s) 547, 604
NruI TCGCGA 1 cut(s) 509
PaeR7I CTCGAG 1 cut(s) 200
PauI GCGCGC 1 cut(s) 477
PcsI WCGNNNNNNNCGW 3 cut(s) 168, 207, 513
PdiI GCCGGC 1 cut(s) 621
PfeI GAWTC 1 cut(s) 690
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 6 cut(s) 67, 297, 401, 406, 476, 483
PleI GAGTC 2 cut(s) 521, 649
PluTI GGCGCC 1 cut(s) 620
PmaCI CACGTG 1 cut(s) 488
PmlI CACGTG 1 cut(s) 488
PpsI GAGTC 2 cut(s) 521, 649
Ppu21I YACGTR 1 cut(s) 488
Psp6I CCWGG 2 cut(s) 176, 258
PspCI CACGTG 1 cut(s) 488
PspEI GGTNACC 2 cut(s) 205, 547
PspFI CCCAGC 1 cut(s) 655
PspGI CCWGG 2 cut(s) 176, 258
PspN4I GGNNCC 2 cut(s) 299, 618
PspPI GGNCC 4 cut(s) 355, 394, 610, 636
PsuI RGATCY 2 cut(s) 451, 495
PteI GCGCGC 1 cut(s) 477
RruI TCGCGA 1 cut(s) 509
RsaI GTAC 1 cut(s) 533
RsaNI GTAC 1 cut(s) 532
Rsr2I CGGWCCG 1 cut(s) 610
RsrII CGGWCCG 1 cut(s) 610
SaqAI TTAA 1 cut(s) 377
SatI GCNGC 6 cut(s) 66, 296, 400, 405, 475, 482
Sau3AI GATC 4 cut(s) 117, 288, 451, 495
Sau96I GGNCC 4 cut(s) 355, 394, 610, 636
SchI GAGTC 2 cut(s) 522, 650
ScrFI CCNGG 3 cut(s) 178, 260, 614
SfaNI GCATC 1 cut(s) 239
SfoI GGCGCC 1 cut(s) 618
Sfr274I CTCGAG 1 cut(s) 200
SgrAI CRCCGGYG 1 cut(s) 619
SinI GGWCC 2 cut(s) 355, 610
SlaI CTCGAG 1 cut(s) 200
SmlI CTYRAG 2 cut(s) 95, 200
SmoI CTYRAG 2 cut(s) 95, 200
Sse9I AATT 1 cut(s) 149
SspDI GGCGCC 1 cut(s) 616
StyD4I CCNGG 3 cut(s) 176, 258, 612
TaaI ACNGT 5 cut(s) 211, 529, 553, 604, 610
TaiI ACGT 3 cut(s) 490, 744, 816
TaqI TCGA 6 cut(s) 51, 72, 116, 147, 201, 291
TasI AATT 1 cut(s) 149
TauI GCSGC 4 cut(s) 68, 298, 402, 484
TfiI GAWTC 1 cut(s) 690
Tru1I TTAA 1 cut(s) 377
Tru9I TTAA 1 cut(s) 377
TseFI GTSAC 2 cut(s) 547, 604
TseI GCWGC 2 cut(s) 404, 474
Tsp45I GTSAC 2 cut(s) 547, 604
TspDTI ATGAA 4 cut(s) 17, 884, 896, 910
TspGWI ACGGA 5 cut(s) 77, 265, 395, 677, 803
VpaK11BI GGWCC 2 cut(s) 355, 610
XapI RAATTY 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 200
ZraI GACGTC 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.