Rh4AG432500

UPF0496 protein 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014732
Physical Location & Seq
Reverse (-)
72707408 .. 72708893
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG432500.1

Sequence Viewer

Length: 954 bp
ATGTTCCTAACGGAGATCAAGAAACACGCCGTTCCGTCATTCACGCCTTTTAAGAAACATTCCGTTCCGAACAAATTTGGCCTCATCACTAACGCCTTCGACGACGCTCTGCTCCGCCGTTTGAAGACGCTTTCTCCGCCGTCGATCAGCTTGTCCTGGCTATCCAAGGCCGTCGATTTTCTCGCCTTTTCGCACTCCGAGGCCAGAAGCTTAATCTCCAATCTCGAGGTAACGGCTTTGGATGATTCTCTTGCGACTTATTTGGATGACAGTGTGAAGCTTTTGGATATATGCAACTCCGTCTCCGCCGAGATCGAGCGGCTCCGGCAGCGCCGGATTGTTCTCGCTTTCGCCGTTCACCTCCTCCGCGACGGGAAGCTCCTGCGAGCTCGGGAGACTTTAACTGAATGGGAGGGCCGCCGCGCCTCGGGATTCGGCAAAGCGGACAATGCGGAGGTGCTGGTCAGGGATCTGGCGCTGGGGCTCGGCAGCGCTCCACGTGGAAAGATCTCGAGCGTTGGGAGAGTCGTGAGGCGGACGATGTACGCCGTCCGATTGGTGACGGTGTTCGTCGCCGGAGCAGTGGTGTCGGCGATGAACGGATCGGCGGAGACTGTGACTGTCCGGGCGCCGGCGGAGTTTTCTTGGGCCGAGTCGTTGAACGAGCTGGAATCGGCGGTCTCCGGCGAGTTGAAACGGCTATTCGGGAAGGAGGCAAAAAGGAAAGGGACCCTATTGGAGGAGGTAGATGACGTGGCTACACGCGTCGCAGAAGCGTGTGAGATAGCGGAAGAAGACACGGAGAGGAGAAACGACGTCGTAAAAGAGGTGGAGGGGGCAATGGGGTCGTTTTCGGATGGGTTGGAAGGGTTGAGTAAAAGGGTGAATGAAATGTTCCATGGTGTTTTGAGTAACAGGAACTACATGTTGGACCATGTCAGGGTCAGAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

317

Amino Acids

34.96

Weight (kDa)

7.8

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 45 - 136 5e-10 Protein ROH1-like
ROH1-like PF05633 165 - 312 1e-09 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36680
malus_domestica MD13G1008300.v1.1
prunus_persica Prupe.1G344800_v2.0.a1
pyrus_communis pycom13g00680
rosa_chinensis RchiOBHm_Chr4g0446391
rosa_laevigata RLG00000005666
rosa_multiflora Rmu_sc0008360.1_g000003 Rmu_sc0010778.1_g000005 Rmu_sc0025512.1_g000002 Rmu_sc0025513.1_g000001
rosa_roxburghii Rroxscaffold_5G00386830
rosa_rugosa Rorug04G0371000
rosa_samantha Rh4AG432500 Rh4BG441700 Rh4CG459000 Rh4DG440400
rosa_wichuraiana Rw4G036940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 819
AccB1I GGYRCC 1 cut(s) 628
AccBSI CCGCTC 1 cut(s) 319
AccII CGCG 3 cut(s) 369, 423, 765
AclWI GGATC 2 cut(s) 477, 610
AcsI RAATTY 1 cut(s) 74
AcvI CACGTG 1 cut(s) 500
AcyI GRCGYC 2 cut(s) 629, 816
AfaI GTAC 1 cut(s) 545
AfeI AGCGCT 1 cut(s) 493
AfiI CCNNNNNNNGG 4 cut(s) 427, 631, 739, 940
AflIII ACRYGT 2 cut(s) 763, 924
AgsI TTSAA 3 cut(s) 124, 661, 694
AjiI CACGTC 1 cut(s) 754
AjnI CCWGG 1 cut(s) 155
AjuI GAANNNNNNNTTGG 2 cut(s) 911, 943
AluBI AGCT 7 cut(s) 150, 210, 280, 379, 389, 667, 950
AluI AGCT 7 cut(s) 150, 210, 280, 379, 389, 667, 950
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 4 cut(s) 307, 389, 605, 685
AlwI GGATC 2 cut(s) 477, 610
Ama87I CYCGRG 4 cut(s) 224, 390, 427, 511
Aor51HI AGCGCT 1 cut(s) 493
AoxI GGCC 5 cut(s) 79, 168, 201, 415, 648
ApeKI GCWGC 2 cut(s) 328, 489
ApoI RAATTY 1 cut(s) 74
AspLEI GCGC 5 cut(s) 333, 425, 478, 494, 631
AspS9I GGNCC 4 cut(s) 415, 648, 729, 931
AsuC2I CCSGG 1 cut(s) 626
AsuHPI GGTGA 3 cut(s) 350, 571, 895
AvaI CYCGRG 4 cut(s) 224, 390, 427, 511
AvaII GGWCC 2 cut(s) 729, 931
BanI GGYRCC 1 cut(s) 628
BanII GRGCYC 2 cut(s) 391, 486
BbrPI CACGTG 1 cut(s) 500
BbsI GAAGAC 2 cut(s) 131, 801
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 2 cut(s) 340, 501
BccI CCATC 1 cut(s) 851
BceAI ACGGC 8 cut(s) 14, 102, 124, 155, 249, 338, 533, 713
BcgI CGANNNNNNTGC 4 cut(s) 570, 604, 828, 862
BciT130I CCWGG 1 cut(s) 157
BcnI CCSGG 1 cut(s) 626
BcoDI GTCTC 4 cut(s) 307, 389, 605, 685
BfoI RGCGCY 4 cut(s) 334, 479, 495, 632
BglII AGATCT 1 cut(s) 507
BisI GCNGC 5 cut(s) 320, 329, 418, 421, 490
BlsI GCNGC 5 cut(s) 321, 330, 419, 422, 491
Bme1390I CCNGG 2 cut(s) 157, 626
Bme18I GGWCC 2 cut(s) 729, 931
BmeT110I CYCGRG 4 cut(s) 224, 390, 427, 511
BmgBI CACGTC 1 cut(s) 754
BmgT120I GGNCC 4 cut(s) 415, 648, 729, 931
BmiI GGNNCC 4 cut(s) 323, 630, 730, 731
BmrFI CCNGG 2 cut(s) 157, 626
BpiI GAAGAC 2 cut(s) 131, 801
BpuMI CCSGG 1 cut(s) 626
BsaAI YACGTR 1 cut(s) 500
BsaHI GRCGYC 2 cut(s) 629, 816
BsaI GGTCTC 1 cut(s) 685
BsaJI CCNNGG 4 cut(s) 165, 198, 426, 898
BsaXI ACNNNNNCTCC 6 cut(s) 118, 148, 287, 317, 570, 600
Bsc4I CCNNNNNNNGG 4 cut(s) 427, 631, 739, 940
Bse118I RCCGGY 1 cut(s) 631
Bse3DI GCAATG 1 cut(s) 846
BseBI CCWGG 1 cut(s) 157
BseDI CCNNGG 4 cut(s) 165, 198, 426, 898
BseGI GGATG 3 cut(s) 247, 271, 862
BseLI CCNNNNNNNGG 4 cut(s) 427, 631, 739, 940
BseMI GCAATG 1 cut(s) 846
BseRI GAGGAG 3 cut(s) 353, 755, 820
BseXI GCAGC 2 cut(s) 340, 501
BseYI CCCAGC 1 cut(s) 478
Bsh1236I CGCG 3 cut(s) 369, 423, 765
BshFI GGCC 5 cut(s) 81, 170, 203, 417, 650
BshNI GGYRCC 1 cut(s) 628
BsiHKAI GWGCWC 1 cut(s) 391
BsiHKCI CYCGRG 4 cut(s) 224, 390, 427, 511
BsiSI CCGG 6 cut(s) 325, 334, 576, 625, 632, 684
BslFI GGGAC 1 cut(s) 742
BslI CCNNNNNNNGG 4 cut(s) 427, 631, 739, 940
BsmAI GTCTC 4 cut(s) 307, 389, 605, 685
BsmBI CGTCTC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 742
BsnI GGCC 5 cut(s) 81, 170, 203, 417, 650
Bso31I GGTCTC 1 cut(s) 685
BsoBI CYCGRG 4 cut(s) 224, 390, 427, 511
Bsp1286I GDGCHC 2 cut(s) 391, 486
Bsp143I GATC 6 cut(s) 15, 144, 312, 469, 507, 602
Bsp19I CCATGG 1 cut(s) 898
BspANI GGCC 5 cut(s) 81, 170, 203, 417, 650
BspFNI CGCG 3 cut(s) 369, 423, 765
BspLI GGNNCC 4 cut(s) 323, 630, 730, 731
BspPI GGATC 2 cut(s) 477, 610
BspT107I GGYRCC 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 685
BsrBI CCGCTC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 846
BsrFI RCCGGY 1 cut(s) 631
BssAI RCCGGY 1 cut(s) 631
BssECI CCNNGG 4 cut(s) 165, 198, 426, 898
BssMI GATC 6 cut(s) 15, 144, 312, 469, 507, 602
BssNI GRCGYC 2 cut(s) 629, 816
BssT1I CCWWGG 2 cut(s) 165, 898
Bst2UI CCWGG 1 cut(s) 157
Bst4CI ACNGT 4 cut(s) 272, 565, 616, 622
BstACI GRCGYC 2 cut(s) 629, 816
BstBAI YACGTR 1 cut(s) 500
BstC8I GCNNGC 2 cut(s) 387, 633
BstDEI CTNAG 1 cut(s) 951
BstDSI CCRYGG 1 cut(s) 898
BstENI CCTNNNNNAGG 1 cut(s) 737
BstF5I GGATG 3 cut(s) 247, 271, 862
BstFNI CGCG 3 cut(s) 369, 423, 765
BstH2I RGCGCY 4 cut(s) 334, 479, 495, 632
BstHHI GCGC 5 cut(s) 333, 425, 478, 494, 631
BstKTI GATC 6 cut(s) 18, 147, 315, 472, 510, 605
BstMAI GTCTC 4 cut(s) 307, 389, 605, 685
BstMBI GATC 6 cut(s) 15, 144, 312, 469, 507, 602
BstMWI GCNNNNNNNGC 4 cut(s) 136, 325, 328, 449
BstNI CCWGG 1 cut(s) 157
BstNSI RCATGY 1 cut(s) 928
BstSCI CCNGG 2 cut(s) 155, 624
BstUI CGCG 3 cut(s) 369, 423, 765
BstV1I GCAGC 2 cut(s) 340, 501
BstV2I GAAGAC 2 cut(s) 131, 801
BstX2I RGATCY 2 cut(s) 469, 507
BstYI RGATCY 2 cut(s) 469, 507
BsuRI GGCC 5 cut(s) 81, 170, 203, 417, 650
BtgI CCRYGG 1 cut(s) 898
BtgZI GCGATG 1 cut(s) 608
BtrI CACGTC 1 cut(s) 754
BtsCI GGATG 3 cut(s) 247, 271, 862
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 2 cut(s) 277, 588
Cac8I GCNNGC 2 cut(s) 387, 633
CfoI GCGC 5 cut(s) 333, 425, 478, 494, 631
Cfr10I RCCGGY 1 cut(s) 631
Cfr13I GGNCC 4 cut(s) 415, 648, 729, 931
CseI GACGC 3 cut(s) 113, 136, 754
Csp6I GTAC 1 cut(s) 544
CviAII CATG 3 cut(s) 899, 925, 935
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 1 cut(s) 951
DinI GGCGCC 1 cut(s) 630
DpnI GATC 6 cut(s) 17, 146, 314, 471, 509, 604
DpnII GATC 6 cut(s) 15, 144, 312, 469, 507, 602
EciI GGCGGA 6 cut(s) 104, 126, 295, 550, 623, 650
Ecl136II GAGCTC 1 cut(s) 389
Eco130I CCWWGG 2 cut(s) 165, 898
Eco24I GRGCYC 2 cut(s) 391, 486
Eco31I GGTCTC 1 cut(s) 685
Eco47I GGWCC 2 cut(s) 729, 931
Eco47III AGCGCT 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 389
Eco72I CACGTG 1 cut(s) 500
Eco88I CYCGRG 4 cut(s) 224, 390, 427, 511
EcoICRI GAGCTC 1 cut(s) 389
EcoNI CCTNNNNNAGG 1 cut(s) 737
EcoO109I RGGNCCY 1 cut(s) 729
EcoRII CCWGG 1 cut(s) 155
EcoT14I CCWWGG 2 cut(s) 165, 898
EcoT38I GRGCYC 2 cut(s) 391, 486
EgeI GGCGCC 1 cut(s) 630
EheI GGCGCC 1 cut(s) 630
ErhI CCWWGG 2 cut(s) 165, 898
Esp3I CGTCTC 1 cut(s) 307
FaeI CATG 3 cut(s) 902, 928, 938
FaiI YATR 5 cut(s) 290, 292, 900, 926, 936
FaqI GGGAC 1 cut(s) 742
FatI CATG 3 cut(s) 898, 924, 934
Fnu4HI GCNGC 5 cut(s) 320, 329, 418, 421, 490
FokI GGATG 3 cut(s) 254, 278, 869
FriOI GRGCYC 2 cut(s) 391, 486
Fsp4HI GCNGC 5 cut(s) 320, 329, 418, 421, 490
GlaI GCGC 5 cut(s) 332, 424, 477, 493, 630
GluI GCNGC 5 cut(s) 320, 329, 418, 421, 490
GsaI CCCAGC 1 cut(s) 482
HaeII RGCGCY 4 cut(s) 334, 479, 495, 632
HaeIII GGCC 5 cut(s) 81, 170, 203, 417, 650
HapII CCGG 6 cut(s) 325, 334, 576, 625, 632, 684
HgaI GACGC 3 cut(s) 113, 136, 754
HhaI GCGC 5 cut(s) 333, 425, 478, 494, 631
Hin1I GRCGYC 2 cut(s) 629, 816
Hin1II CATG 3 cut(s) 902, 928, 938
Hin6I GCGC 5 cut(s) 331, 423, 476, 492, 629
HinP1I GCGC 5 cut(s) 331, 423, 476, 492, 629
HindIII AAGCTT 2 cut(s) 208, 278
HinfI GANTC 5 cut(s) 245, 432, 525, 653, 671
HpaII CCGG 6 cut(s) 325, 334, 576, 625, 632, 684
HphI GGTGA 3 cut(s) 350, 571, 895
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 5 cut(s) 69, 199, 554, 856, 947
Hpy188III TCNNGA 7 cut(s) 19, 224, 392, 429, 511, 529, 706
Hpy8I GTNNAC 1 cut(s) 358
Hpy99I CGWCG 9 cut(s) 104, 107, 145, 176, 374, 575, 770, 818, 821
HpyAV CCTTC 3 cut(s) 106, 703, 860
HpyCH4III ACNGT 4 cut(s) 272, 565, 616, 622
HpyCH4IV ACGT 3 cut(s) 499, 753, 816
HpyCH4V TGCA 1 cut(s) 294
HpyF10VI GCNNNNNNNGC 4 cut(s) 136, 325, 328, 449
HpyF3I CTNAG 1 cut(s) 951
HpySE526I ACGT 3 cut(s) 499, 753, 816
Hsp92I GRCGYC 2 cut(s) 629, 816
Hsp92II CATG 3 cut(s) 902, 928, 938
HspAI GCGC 5 cut(s) 331, 423, 476, 492, 629
KasI GGCGCC 1 cut(s) 628
KflI GGGWCCC 1 cut(s) 729
KroI GCCGGC 1 cut(s) 631
KroNI GCCGGC 1 cut(s) 633
Kzo9I GATC 6 cut(s) 15, 144, 312, 469, 507, 602
LmnI GCTCC 5 cut(s) 117, 327, 384, 499, 578
Lsp1109I GCAGC 2 cut(s) 340, 501
MaeII ACGT 3 cut(s) 499, 753, 816
MaeIII GTNAC 4 cut(s) 229, 559, 616, 911
MalI GATC 6 cut(s) 17, 146, 314, 471, 509, 604
MbiI CCGCTC 1 cut(s) 319
MboI GATC 6 cut(s) 15, 144, 312, 469, 507, 602
MboII GAAGA 3 cut(s) 136, 803, 806
MflI RGATCY 2 cut(s) 469, 507
MhlI GDGCHC 2 cut(s) 391, 486
MluCI AATT 1 cut(s) 74
MluI ACGCGT 1 cut(s) 763
Mly113I GGCGCC 1 cut(s) 629
MlyI GAGTC 2 cut(s) 534, 662
MmeI TCCRAC 2 cut(s) 843, 909
MreI CGCCGGCG 1 cut(s) 631
MroNI GCCGGC 1 cut(s) 631
MseI TTAA 3 cut(s) 51, 212, 401
MspI CCGG 6 cut(s) 325, 334, 576, 625, 632, 684
MspR9I CCNGG 2 cut(s) 157, 626
MvaI CCWGG 1 cut(s) 157
MvnI CGCG 3 cut(s) 369, 423, 765
MwoI GCNNNNNNNGC 4 cut(s) 136, 325, 328, 449
NaeI GCCGGC 1 cut(s) 633
NarI GGCGCC 1 cut(s) 629
NciI CCSGG 1 cut(s) 626
NcoI CCATGG 1 cut(s) 898
NdeII GATC 6 cut(s) 15, 144, 312, 469, 507, 602
NgoMIV GCCGGC 1 cut(s) 631
NlaIII CATG 3 cut(s) 902, 928, 938
NlaIV GGNNCC 4 cut(s) 323, 630, 730, 731
NmeAIII GCCGAG 3 cut(s) 334, 465, 676
NmuCI GTSAC 2 cut(s) 559, 616
NspI RCATGY 1 cut(s) 928
PaeR7I CTCGAG 2 cut(s) 224, 511
PciI ACATGT 1 cut(s) 924
PcsI WCGNNNNNNNCGW 2 cut(s) 99, 351
PdiI GCCGGC 1 cut(s) 633
PfeI GAWTC 3 cut(s) 245, 432, 671
PflFI GACNNNGTC 1 cut(s) 935
PkrI GCNGC 5 cut(s) 321, 330, 419, 422, 491
PleI GAGTC 2 cut(s) 533, 661
PluTI GGCGCC 1 cut(s) 632
PmaCI CACGTG 1 cut(s) 500
PmlI CACGTG 1 cut(s) 500
PpsI GAGTC 2 cut(s) 533, 661
Ppu21I YACGTR 1 cut(s) 500
PpuMI RGGWCCY 1 cut(s) 729
PscI ACATGT 1 cut(s) 924
Psp124BI GAGCTC 1 cut(s) 391
Psp5II RGGWCCY 1 cut(s) 729
Psp6I CCWGG 1 cut(s) 155
PspCI CACGTG 1 cut(s) 500
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 1 cut(s) 155
PspN4I GGNNCC 4 cut(s) 323, 630, 730, 731
PspPI GGNCC 4 cut(s) 415, 648, 729, 931
PspPPI RGGWCCY 1 cut(s) 729
PsuI RGATCY 2 cut(s) 469, 507
PsyI GACNNNGTC 1 cut(s) 935
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
SacI GAGCTC 1 cut(s) 391
SaqAI TTAA 3 cut(s) 51, 212, 401
SatI GCNGC 5 cut(s) 320, 329, 418, 421, 490
Sau3AI GATC 6 cut(s) 15, 144, 312, 469, 507, 602
Sau96I GGNCC 4 cut(s) 415, 648, 729, 931
SchI GAGTC 2 cut(s) 534, 662
ScrFI CCNGG 2 cut(s) 157, 626
SduI GDGCHC 2 cut(s) 391, 486
SfoI GGCGCC 1 cut(s) 630
Sfr274I CTCGAG 2 cut(s) 224, 511
SgrAI CRCCGGYG 1 cut(s) 631
SinI GGWCC 2 cut(s) 729, 931
SlaI CTCGAG 2 cut(s) 224, 511
SmlI CTYRAG 2 cut(s) 224, 511
SmoI CTYRAG 2 cut(s) 224, 511
Sse9I AATT 1 cut(s) 74
SspDI GGCGCC 1 cut(s) 628
SstI GAGCTC 1 cut(s) 391
StyD4I CCNGG 2 cut(s) 155, 624
StyI CCWWGG 2 cut(s) 165, 898
TaaI ACNGT 4 cut(s) 272, 565, 616, 622
TaiI ACGT 3 cut(s) 502, 756, 819
TaqI TCGA 6 cut(s) 99, 143, 174, 225, 315, 512
TasI AATT 1 cut(s) 74
TauI GCSGC 3 cut(s) 322, 420, 423
TfiI GAWTC 3 cut(s) 245, 432, 671
Tru1I TTAA 3 cut(s) 51, 212, 401
Tru9I TTAA 3 cut(s) 51, 212, 401
TscAI CASTG 2 cut(s) 277, 588
TseFI GTSAC 2 cut(s) 559, 616
TseI GCWGC 2 cut(s) 328, 489
Tsp45I GTSAC 2 cut(s) 559, 616
TspDTI ATGAA 2 cut(s) 611, 903
TspGWI ACGGA 6 cut(s) 24, 26, 52, 289, 615, 815
TspRI CASTG 2 cut(s) 277, 588
Tth111I GACNNNGTC 1 cut(s) 935
VpaK11BI GGWCC 2 cut(s) 729, 931
XagI CCTNNNNNAGG 1 cut(s) 737
XapI RAATTY 1 cut(s) 74
XceI RCATGY 1 cut(s) 928
XhoI CTCGAG 2 cut(s) 224, 511
ZraI GACGTC 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.