Rmu_sc0025512.1_g000002

UPF0496 protein 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0025512.1
OG0014732
Physical Location & Seq
Reverse (-)
1823 .. 2507
685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0025512.1_g000002.1.cds

Sequence Viewer

Length: 685 bp
cagtgtgaagcttttggatatatgcaactccgtctccgccgagatcgagcggctccggcagcgccggattgttctcgctttagccgttcacctcctcggcgacgggaagctccggcgagctcgggagactttaactgaatgggagggccgccgcgcctcgggattcgggaaagcgaacaatgcggaggtgctggtcagggatctggcgctggggctccgcagcgcgccacgtggaaagatctcgagcgttgggagagtcgtgcggcggacgatgtacgccgtccgattgttgacggtgttcgtcgccggagcagttttgtcggcgatgaaaggatcggcggagactgtgactgtccgggcgccggcggagttttcttgggccgagtcgttgaacgagctggaatcgggggtctccggcgagttgaaacggcgattcgggaaggaggcaaaaaggaaagggaccctattggaggaggtagatgaggtggctacacgcgtcgcagaagcgtgtgagatagcggaagaagacacggagaagagaaacgacgtcgtaaaagaggtggagagggcaatggggtcgttttcggatgggttggaagcgttgagtaaaagggtgaatgaaatgttccatggtgttttgagtaacaggaactacatgttggaccatgtcagggtcagagcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

227

Amino Acids

25.12

Weight (kDa)

9.51

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36680
malus_domestica MD13G1008300.v1.1
prunus_persica Prupe.1G344800_v2.0.a1
pyrus_communis pycom13g00680
rosa_chinensis RchiOBHm_Chr4g0446391
rosa_laevigata RLG00000005666
rosa_multiflora Rmu_sc0008360.1_g000003 Rmu_sc0010778.1_g000005 Rmu_sc0025512.1_g000002 Rmu_sc0025513.1_g000001
rosa_roxburghii Rroxscaffold_5G00386830
rosa_rugosa Rorug04G0371000
rosa_samantha Rh4AG432500 Rh4BG441700 Rh4CG459000 Rh4DG440400
rosa_wichuraiana Rw4G036940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 550
AccB1I GGYRCC 1 cut(s) 359
AccBSI CCGCTC 1 cut(s) 50
AccII CGCG 3 cut(s) 154, 225, 496
AclWI GGATC 2 cut(s) 208, 341
AcvI CACGTG 1 cut(s) 231
AcyI GRCGYC 2 cut(s) 360, 547
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 4 cut(s) 158, 362, 470, 671
AflIII ACRYGT 2 cut(s) 494, 655
AgsI TTSAA 2 cut(s) 392, 425
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AluBI AGCT 5 cut(s) 11, 110, 120, 398, 681
AluI AGCT 5 cut(s) 11, 110, 120, 398, 681
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 4 cut(s) 38, 120, 336, 416
AlwI GGATC 2 cut(s) 208, 341
Ama87I CYCGRG 3 cut(s) 121, 158, 242
AoxI GGCC 2 cut(s) 146, 379
ApeKI GCWGC 2 cut(s) 59, 220
AspLEI GCGC 6 cut(s) 64, 156, 209, 225, 227, 362
AspS9I GGNCC 4 cut(s) 146, 379, 460, 662
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 2 cut(s) 81, 626
AvaI CYCGRG 3 cut(s) 121, 158, 242
AvaII GGWCC 2 cut(s) 460, 662
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 2 cut(s) 122, 217
BbrPI CACGTG 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 532
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 2 cut(s) 71, 232
BccI CCATC 1 cut(s) 582
BceAI ACGGC 3 cut(s) 69, 264, 444
BcgI CGANNNNNNTGC 4 cut(s) 301, 335, 559, 593
BcnI CCSGG 1 cut(s) 357
BcoDI GTCTC 4 cut(s) 38, 120, 336, 416
BfoI RGCGCY 3 cut(s) 65, 210, 363
BglII AGATCT 1 cut(s) 238
BisI GCNGC 6 cut(s) 51, 60, 149, 152, 221, 264
BlsI GCNGC 6 cut(s) 52, 61, 150, 153, 222, 265
Bme1390I CCNGG 1 cut(s) 357
Bme18I GGWCC 2 cut(s) 460, 662
BmeT110I CYCGRG 3 cut(s) 121, 158, 242
BmgT120I GGNCC 4 cut(s) 146, 379, 460, 662
BmiI GGNNCC 5 cut(s) 54, 216, 361, 461, 462
BmrFI CCNGG 1 cut(s) 357
BpiI GAAGAC 1 cut(s) 532
BpuMI CCSGG 1 cut(s) 357
BsaAI YACGTR 1 cut(s) 231
BsaHI GRCGYC 2 cut(s) 360, 547
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 3 cut(s) 95, 157, 629
BsaXI ACNNNNNCTCC 2 cut(s) 18, 48
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 362, 470, 671
Bse118I RCCGGY 1 cut(s) 362
Bse3DI GCAATG 1 cut(s) 577
BseDI CCNNGG 3 cut(s) 95, 157, 629
BseGI GGATG 1 cut(s) 593
BseLI CCNNNNNNNGG 4 cut(s) 158, 362, 470, 671
BseMI GCAATG 1 cut(s) 577
BsePI GCGCGC 1 cut(s) 223
BseRI GAGGAG 2 cut(s) 84, 486
BseXI GCAGC 2 cut(s) 71, 232
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 3 cut(s) 154, 225, 496
BshFI GGCC 2 cut(s) 148, 381
BshNI GGYRCC 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 122
BsiHKCI CYCGRG 3 cut(s) 121, 158, 242
BsiSI CCGG 7 cut(s) 56, 65, 113, 307, 356, 363, 415
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 4 cut(s) 158, 362, 470, 671
BsmAI GTCTC 4 cut(s) 38, 120, 336, 416
BsmBI CGTCTC 1 cut(s) 38
BsmFI GGGAC 1 cut(s) 473
BsnI GGCC 2 cut(s) 148, 381
Bso31I GGTCTC 1 cut(s) 416
BsoBI CYCGRG 3 cut(s) 121, 158, 242
Bsp1286I GDGCHC 2 cut(s) 122, 217
Bsp143I GATC 4 cut(s) 43, 200, 238, 333
Bsp19I CCATGG 1 cut(s) 629
BspANI GGCC 2 cut(s) 148, 381
BspFNI CGCG 3 cut(s) 154, 225, 496
BspLI GGNNCC 5 cut(s) 54, 216, 361, 461, 462
BspPI GGATC 2 cut(s) 208, 341
BspT107I GGYRCC 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 416
BsrBI CCGCTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 577
BsrFI RCCGGY 1 cut(s) 362
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 95, 157, 629
BssHII GCGCGC 1 cut(s) 223
BssMI GATC 4 cut(s) 43, 200, 238, 333
BssNI GRCGYC 2 cut(s) 360, 547
BssT1I CCWWGG 1 cut(s) 629
Bst4CI ACNGT 3 cut(s) 296, 347, 353
Bst6I CTCTTC 1 cut(s) 531
BstACI GRCGYC 2 cut(s) 360, 547
BstBAI YACGTR 1 cut(s) 231
BstC8I GCNNGC 3 cut(s) 118, 225, 364
BstDEI CTNAG 1 cut(s) 682
BstDSI CCRYGG 1 cut(s) 629
BstENI CCTNNNNNAGG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 593
BstFNI CGCG 3 cut(s) 154, 225, 496
BstH2I RGCGCY 3 cut(s) 65, 210, 363
BstHHI GCGC 6 cut(s) 64, 156, 209, 225, 227, 362
BstKTI GATC 4 cut(s) 46, 203, 241, 336
BstMAI GTCTC 4 cut(s) 38, 120, 336, 416
BstMBI GATC 4 cut(s) 43, 200, 238, 333
BstMWI GCNNNNNNNGC 3 cut(s) 56, 59, 180
BstNSI RCATGY 1 cut(s) 659
BstSCI CCNGG 1 cut(s) 355
BstUI CGCG 3 cut(s) 154, 225, 496
BstV1I GCAGC 2 cut(s) 71, 232
BstV2I GAAGAC 1 cut(s) 532
BstX2I RGATCY 2 cut(s) 200, 238
BstYI RGATCY 2 cut(s) 200, 238
BsuRI GGCC 2 cut(s) 148, 381
BtgI CCRYGG 1 cut(s) 629
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 1 cut(s) 593
BtsIMutI CAGTG 1 cut(s) 8
Cac8I GCNNGC 3 cut(s) 118, 225, 364
CfoI GCGC 6 cut(s) 64, 156, 209, 225, 227, 362
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 4 cut(s) 146, 379, 460, 662
CseI GACGC 1 cut(s) 485
Csp6I GTAC 1 cut(s) 275
CviAII CATG 3 cut(s) 630, 656, 666
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 1 cut(s) 682
DinI GGCGCC 1 cut(s) 361
DpnI GATC 4 cut(s) 45, 202, 240, 335
DpnII GATC 4 cut(s) 43, 200, 238, 333
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
EciI GGCGGA 4 cut(s) 26, 281, 354, 381
Ecl136II GAGCTC 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 629
Eco24I GRGCYC 2 cut(s) 122, 217
Eco31I GGTCTC 1 cut(s) 416
Eco47I GGWCC 2 cut(s) 460, 662
Eco53kI GAGCTC 1 cut(s) 120
Eco72I CACGTG 1 cut(s) 231
Eco88I CYCGRG 3 cut(s) 121, 158, 242
EcoICRI GAGCTC 1 cut(s) 120
EcoNI CCTNNNNNAGG 1 cut(s) 468
EcoO109I RGGNCCY 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 629
EcoT38I GRGCYC 2 cut(s) 122, 217
EgeI GGCGCC 1 cut(s) 361
EheI GGCGCC 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 629
Esp3I CGTCTC 1 cut(s) 38
FaeI CATG 3 cut(s) 633, 659, 669
FaiI YATR 5 cut(s) 21, 23, 631, 657, 667
FaqI GGGAC 1 cut(s) 473
FatI CATG 3 cut(s) 629, 655, 665
Fnu4HI GCNGC 6 cut(s) 51, 60, 149, 152, 221, 264
FokI GGATG 1 cut(s) 600
FriOI GRGCYC 2 cut(s) 122, 217
Fsp4HI GCNGC 6 cut(s) 51, 60, 149, 152, 221, 264
GlaI GCGC 6 cut(s) 63, 155, 208, 224, 226, 361
GluI GCNGC 6 cut(s) 51, 60, 149, 152, 221, 264
GsaI CCCAGC 1 cut(s) 213
HaeII RGCGCY 3 cut(s) 65, 210, 363
HaeIII GGCC 2 cut(s) 148, 381
HapII CCGG 7 cut(s) 56, 65, 113, 307, 356, 363, 415
HgaI GACGC 1 cut(s) 485
HhaI GCGC 6 cut(s) 64, 156, 209, 225, 227, 362
Hin1I GRCGYC 2 cut(s) 360, 547
Hin1II CATG 3 cut(s) 633, 659, 669
Hin6I GCGC 6 cut(s) 62, 154, 207, 223, 225, 360
HinP1I GCGC 6 cut(s) 62, 154, 207, 223, 225, 360
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HindIII AAGCTT 1 cut(s) 9
HinfI GANTC 5 cut(s) 163, 256, 384, 402, 433
HpaII CCGG 7 cut(s) 56, 65, 113, 307, 356, 363, 415
HphI GGTGA 2 cut(s) 81, 626
Hpy166II GTNNAC 2 cut(s) 89, 292
Hpy188I TCNGA 3 cut(s) 285, 587, 678
Hpy188III TCNNGA 5 cut(s) 123, 160, 167, 242, 437
Hpy8I GTNNAC 2 cut(s) 89, 292
Hpy99I CGWCG 5 cut(s) 105, 306, 501, 549, 552
HpyAV CCTTC 1 cut(s) 434
HpyCH4III ACNGT 3 cut(s) 296, 347, 353
HpyCH4IV ACGT 2 cut(s) 230, 547
HpyCH4V TGCA 1 cut(s) 25
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 59, 180
HpyF3I CTNAG 1 cut(s) 682
HpySE526I ACGT 2 cut(s) 230, 547
Hsp92I GRCGYC 2 cut(s) 360, 547
Hsp92II CATG 3 cut(s) 633, 659, 669
HspAI GCGC 6 cut(s) 62, 154, 207, 223, 225, 360
KasI GGCGCC 1 cut(s) 359
KflI GGGWCCC 1 cut(s) 460
KroI GCCGGC 1 cut(s) 362
KroNI GCCGGC 1 cut(s) 364
Kzo9I GATC 4 cut(s) 43, 200, 238, 333
LmnI GCTCC 4 cut(s) 58, 115, 220, 309
Lsp1109I GCAGC 2 cut(s) 71, 232
MaeII ACGT 2 cut(s) 230, 547
MaeIII GTNAC 2 cut(s) 347, 642
MalI GATC 4 cut(s) 45, 202, 240, 335
MbiI CCGCTC 1 cut(s) 50
MboI GATC 4 cut(s) 43, 200, 238, 333
MboII GAAGA 3 cut(s) 534, 537, 548
MflI RGATCY 2 cut(s) 200, 238
MhlI GDGCHC 2 cut(s) 122, 217
MluI ACGCGT 1 cut(s) 494
Mly113I GGCGCC 1 cut(s) 360
MlyI GAGTC 2 cut(s) 265, 393
MmeI TCCRAC 2 cut(s) 574, 640
MreI CGCCGGCG 1 cut(s) 362
MroNI GCCGGC 1 cut(s) 362
MseI TTAA 1 cut(s) 132
MspI CCGG 7 cut(s) 56, 65, 113, 307, 356, 363, 415
MspR9I CCNGG 1 cut(s) 357
MvnI CGCG 3 cut(s) 154, 225, 496
MwoI GCNNNNNNNGC 3 cut(s) 56, 59, 180
NaeI GCCGGC 1 cut(s) 364
NarI GGCGCC 1 cut(s) 360
NciI CCSGG 1 cut(s) 357
NcoI CCATGG 1 cut(s) 629
NdeII GATC 4 cut(s) 43, 200, 238, 333
NgoMIV GCCGGC 1 cut(s) 362
NlaIII CATG 3 cut(s) 633, 659, 669
NlaIV GGNNCC 5 cut(s) 54, 216, 361, 461, 462
NmeAIII GCCGAG 3 cut(s) 65, 76, 407
NmuCI GTSAC 1 cut(s) 347
NspI RCATGY 1 cut(s) 659
PaeR7I CTCGAG 1 cut(s) 242
PauI GCGCGC 1 cut(s) 223
PciI ACATGT 1 cut(s) 655
PcsI WCGNNNNNNNCGW 1 cut(s) 82
PdiI GCCGGC 1 cut(s) 364
PfeI GAWTC 3 cut(s) 163, 402, 433
PflFI GACNNNGTC 1 cut(s) 666
PkrI GCNGC 6 cut(s) 52, 61, 150, 153, 222, 265
PleI GAGTC 2 cut(s) 264, 392
PluTI GGCGCC 1 cut(s) 363
PmaCI CACGTG 1 cut(s) 231
PmlI CACGTG 1 cut(s) 231
PpsI GAGTC 2 cut(s) 264, 392
Ppu21I YACGTR 1 cut(s) 231
PpuMI RGGWCCY 1 cut(s) 460
PscI ACATGT 1 cut(s) 655
Psp124BI GAGCTC 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 460
PspCI CACGTG 1 cut(s) 231
PspFI CCCAGC 1 cut(s) 209
PspN4I GGNNCC 5 cut(s) 54, 216, 361, 461, 462
PspPI GGNCC 4 cut(s) 146, 379, 460, 662
PspPPI RGGWCCY 1 cut(s) 460
PsuI RGATCY 2 cut(s) 200, 238
PsyI GACNNNGTC 1 cut(s) 666
PteI GCGCGC 1 cut(s) 223
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
SacI GAGCTC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 6 cut(s) 51, 60, 149, 152, 221, 264
Sau3AI GATC 4 cut(s) 43, 200, 238, 333
Sau96I GGNCC 4 cut(s) 146, 379, 460, 662
SchI GAGTC 2 cut(s) 265, 393
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 2 cut(s) 122, 217
SfoI GGCGCC 1 cut(s) 361
Sfr274I CTCGAG 1 cut(s) 242
SgrAI CRCCGGYG 1 cut(s) 362
SinI GGWCC 2 cut(s) 460, 662
SlaI CTCGAG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
SspDI GGCGCC 1 cut(s) 359
SstI GAGCTC 1 cut(s) 122
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 1 cut(s) 629
TaaI ACNGT 3 cut(s) 296, 347, 353
TaiI ACGT 2 cut(s) 233, 550
TaqI TCGA 2 cut(s) 46, 243
TauI GCSGC 4 cut(s) 53, 151, 154, 266
TfiI GAWTC 3 cut(s) 163, 402, 433
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 2 cut(s) 59, 220
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 2 cut(s) 342, 634
TspGWI ACGGA 2 cut(s) 20, 546
Tth111I GACNNNGTC 1 cut(s) 666
VpaK11BI GGWCC 2 cut(s) 460, 662
XagI CCTNNNNNAGG 1 cut(s) 468
XceI RCATGY 1 cut(s) 659
XhoI CTCGAG 1 cut(s) 242
ZraI GACGTC 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.