MD13G1008300.v1.1

UPF0496 protein 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014732
Physical Location & Seq
Forward (+)
509522 .. 510571
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1008300.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGTACCTAACGGAAATCTCCAACTTCCCGTCACTCCTTCCCTTCCGGGGTGCTAAGAAACATTCCGTTCCGTCCAATTTGGATCTCATCTCTAGCTCCTTCGATGACGCTGTGCTCCGCCACTTGAAATCTCTCGGTCCGCCGTCCATCAGCTTGTCCTGGCTCTCCAAGGCCGTTGATTTTCTCGCCTTGACTCACTCCGAAGCGAGAAACCTCATCTCCGATCTCAAGGTCGCGCCGCCTCTAGATGATTCGCTCGCTTGGTATTTGGACCATAGCGTCAAGCTCTTGGACCTCTGCAACTCCATCTCTGCCGAGATCGAACGCCTTCGCCAGCTCCGCCTTCATCTCACCTTCGTTCTTCACCTTCTCGGCGACCGAAAGGGTGAAGAGCTCCCGTCGCCGGAGAATCTCCGCCGATCTCGCATGTCTCTATCCGATTTCGATCGCGGTGCTTCTTCTGGCTTTGGAAAGCGGAGCAAGGCCGAAGTCTTGGTCAGAGATCTGAACTTGGGCCTGACTCGCCTGAGCAACGCGCCACGTGGCAAGATCTCCAGCGTAGCAAAGCTCGTGCGTCGAACAGTGCGCGCGGTGGGACTGGTGACTGTGTTCATCGCCGGAATCGCGGTGTCGACAATCCACGGATCTCCGGATATGGTTAGGGTCCGAGCCTCGGCGGAGTTTACATGGGCCGAGTCGTTCAACGAGCTTGAATCGGCAGTCTGGGCCGAGTTGAAACGGCGATTCGGGCGCGGCAATGACGAGCAACTGGAAGGGATGTTGGAAGAGTTGGATGACGTGGGAACACGTGTTAAGGAGGCGTGTGGGGTCGTGGATGACCTGGCAGGAGTTGCGGAAGTGAACAAGGAGGAGAAGGAGAGGTTGAACGAGGTCGTAAAAGAGCTGGAGAAGGCAACGGCGTCGTTTTCGGATGGGTTGGATAGGTTGAGTAACGGAGTGAACGAGATGTTTGATGGTGTTTTGCGCAACAGAAACCATATGACGGAGAATGTTAGAGTTAGCGGAGTGGGGAAAAAGCCAAGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

350

Amino Acids

38.65

Weight (kDa)

7.12

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 48 - 148 1.8e-08 Protein ROH1-like
ROH1-like PF05633 180 - 336 6.4e-07 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36680
malus_domestica MD13G1008300.v1.1
prunus_persica Prupe.1G344800_v2.0.a1
pyrus_communis pycom13g00680
rosa_chinensis RchiOBHm_Chr4g0446391
rosa_laevigata RLG00000005666
rosa_multiflora Rmu_sc0008360.1_g000003 Rmu_sc0010778.1_g000005 Rmu_sc0025512.1_g000002 Rmu_sc0025513.1_g000001
rosa_roxburghii Rroxscaffold_5G00386830
rosa_rugosa Rorug04G0371000
rosa_samantha Rh4AG432500 Rh4BG441700 Rh4CG459000 Rh4DG440400
rosa_wichuraiana Rw4G036940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
Acc16I TGCGCA 1 cut(s) 986
AccI GTMKAC 1 cut(s) 632
AccII CGCG 7 cut(s) 236, 450, 536, 588, 590, 626, 753
AccIII TCCGGA 1 cut(s) 649
AclWI GGATC 2 cut(s) 90, 652
AcvI CACGTG 2 cut(s) 542, 809
AcyI GRCGYC 1 cut(s) 920
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 4 cut(s) 47, 403, 673, 1003
AflIII ACRYGT 2 cut(s) 806, 808
AgsI TTSAA 5 cut(s) 127, 703, 713, 736, 886
AjiI CACGTC 1 cut(s) 799
AjnI CCWGG 2 cut(s) 158, 840
AluBI AGCT 8 cut(s) 96, 153, 286, 337, 394, 568, 709, 904
AluI AGCT 8 cut(s) 96, 153, 286, 337, 394, 568, 709, 904
Alw21I GWGCWC 2 cut(s) 117, 396
Alw26I GTCTC 1 cut(s) 435
AlwI GGATC 2 cut(s) 90, 652
Aor13HI TCCGGA 1 cut(s) 649
AoxI GGCC 5 cut(s) 171, 483, 514, 690, 726
ArsI GACNNNNNNTTYG 2 cut(s) 480, 512
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 6 cut(s) 238, 538, 588, 590, 753, 987
AspS9I GGNCC 7 cut(s) 137, 271, 292, 514, 664, 690, 726
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 4 cut(s) 343, 356, 398, 613
AvaII GGWCC 4 cut(s) 137, 271, 292, 664
BanII GRGCYC 1 cut(s) 396
BauI CACGAG 1 cut(s) 569
BbrPI CACGTG 2 cut(s) 542, 809
Bbv12I GWGCWC 2 cut(s) 117, 396
BccI CCATC 4 cut(s) 155, 314, 926, 968
BceAI ACGGC 4 cut(s) 127, 158, 755, 933
BcgI CGANNNNNNTGC 4 cut(s) 434, 468, 903, 937
BciT130I CCWGG 2 cut(s) 160, 842
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 2 cut(s) 93, 245
BglII AGATCT 2 cut(s) 502, 549
BisI GCNGC 2 cut(s) 239, 754
BlsI GCNGC 2 cut(s) 240, 755
Bme1390I CCNGG 3 cut(s) 47, 160, 842
Bme18I GGWCC 4 cut(s) 137, 271, 292, 664
BmgBI CACGTC 1 cut(s) 799
BmgT120I GGNCC 7 cut(s) 137, 271, 292, 514, 664, 690, 726
BmiI GGNNCC 1 cut(s) 665
BmrFI CCNGG 3 cut(s) 47, 160, 842
BpmI CTGGAG 2 cut(s) 538, 926
Bpu10I CCTNAGC 1 cut(s) 527
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 47
BsaAI YACGTR 2 cut(s) 542, 809
BsaBI GATNNNNATC 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 920
BsaJI CCNNGG 4 cut(s) 46, 168, 640, 672
BsaWI WCCGGW 1 cut(s) 649
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 403, 673, 1003
Bse1I ACTGG 2 cut(s) 603, 774
Bse3DI GCAATG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 444
BseAI TCCGGA 1 cut(s) 649
BseBI CCWGG 2 cut(s) 160, 842
BseDI CCNNGG 4 cut(s) 46, 168, 640, 672
BseGI GGATG 4 cut(s) 783, 799, 841, 937
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 4 cut(s) 47, 403, 673, 1003
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 1 cut(s) 518
BseNI ACTGG 2 cut(s) 603, 774
BsePI GCGCGC 1 cut(s) 586
BseRI GAGGAG 1 cut(s) 884
Bsh1236I CGCG 7 cut(s) 236, 450, 536, 588, 590, 626, 753
Bsh1285I CGRYCG 2 cut(s) 379, 448
BshFI GGCC 5 cut(s) 173, 485, 516, 692, 728
BsiEI CGRYCG 2 cut(s) 379, 448
BsiHKAI GWGCWC 2 cut(s) 117, 396
BsiSI CCGG 4 cut(s) 46, 404, 618, 650
BslFI GGGAC 1 cut(s) 609
BslI CCNNNNNNNGG 4 cut(s) 47, 403, 673, 1003
BsmAI GTCTC 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 609
BsnI GGCC 5 cut(s) 173, 485, 516, 692, 728
Bsp1286I GDGCHC 2 cut(s) 117, 396
Bsp13I TCCGGA 1 cut(s) 649
Bsp143I GATC 8 cut(s) 82, 223, 318, 419, 445, 502, 549, 644
BspANI GGCC 5 cut(s) 173, 485, 516, 692, 728
BspCNI CTCAG 1 cut(s) 519
BspEI TCCGGA 1 cut(s) 649
BspFNI CGCG 7 cut(s) 236, 450, 536, 588, 590, 626, 753
BspLI GGNNCC 1 cut(s) 665
BspPI GGATC 2 cut(s) 90, 652
BspQI GCTCTTC 1 cut(s) 384
BsrDI GCAATG 1 cut(s) 763
BsrI ACTGG 2 cut(s) 603, 774
BssECI CCNNGG 4 cut(s) 46, 168, 640, 672
BssHII GCGCGC 1 cut(s) 586
BssMI GATC 8 cut(s) 82, 223, 318, 419, 445, 502, 549, 644
BssNI GRCGYC 1 cut(s) 920
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 1 cut(s) 168
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 2 cut(s) 160, 842
Bst4CI ACNGT 2 cut(s) 583, 607
Bst6I CTCTTC 2 cut(s) 384, 780
BstACI GRCGYC 1 cut(s) 920
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBAI YACGTR 2 cut(s) 542, 809
BstC8I GCNNGC 3 cut(s) 258, 335, 588
BstDEI CTNAG 2 cut(s) 54, 527
BstDSI CCRYGG 1 cut(s) 640
BstF5I GGATG 4 cut(s) 783, 799, 841, 937
BstFNI CGCG 7 cut(s) 236, 450, 536, 588, 590, 626, 753
BstHHI GCGC 6 cut(s) 238, 538, 588, 590, 753, 987
BstKTI GATC 8 cut(s) 85, 226, 321, 422, 448, 505, 552, 647
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 8 cut(s) 82, 223, 318, 419, 445, 502, 549, 644
BstMCI CGRYCG 2 cut(s) 379, 448
BstMWI GCNNNNNNNGC 8 cut(s) 339, 400, 423, 522, 623, 725, 748, 851
BstNI CCWGG 2 cut(s) 160, 842
BstNSI RCATGY 1 cut(s) 430
BstSCI CCNGG 3 cut(s) 45, 158, 840
BstUI CGCG 7 cut(s) 236, 450, 536, 588, 590, 626, 753
BstX2I RGATCY 4 cut(s) 82, 502, 549, 644
BstYI RGATCY 4 cut(s) 82, 502, 549, 644
BsuRI GGCC 5 cut(s) 173, 485, 516, 692, 728
BtgI CCRYGG 1 cut(s) 640
BtgZI GCGATG 1 cut(s) 598
BtrI CACGTC 1 cut(s) 799
BtsCI GGATG 4 cut(s) 783, 799, 841, 937
BtsIMutI CAGTG 1 cut(s) 588
Cac8I GCNNGC 3 cut(s) 258, 335, 588
CfoI GCGC 6 cut(s) 238, 538, 588, 590, 753, 987
Cfr13I GGNCC 7 cut(s) 137, 271, 292, 514, 664, 690, 726
CpoI CGGWCCG 1 cut(s) 137
CseI GACGC 4 cut(s) 116, 268, 563, 909
Csp6I GTAC 1 cut(s) 4
CspI CGGWCCG 1 cut(s) 137
CviAII CATG 2 cut(s) 427, 687
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 2 cut(s) 54, 527
DpnI GATC 8 cut(s) 84, 225, 320, 421, 447, 504, 551, 646
DpnII GATC 8 cut(s) 82, 223, 318, 419, 445, 502, 549, 644
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
Eam1104I CTCTTC 2 cut(s) 384, 780
EarI CTCTTC 2 cut(s) 384, 780
EciI GGCGGA 5 cut(s) 107, 129, 329, 404, 692
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 396
Eco47I GGWCC 4 cut(s) 137, 271, 292, 664
Eco53kI GAGCTC 1 cut(s) 394
Eco72I CACGTG 2 cut(s) 542, 809
EcoICRI GAGCTC 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 158, 840
EcoT14I CCWWGG 1 cut(s) 168
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 2 cut(s) 430, 690
FaiI YATR 6 cut(s) 276, 428, 656, 688, 999, 1001
FaqI GGGAC 1 cut(s) 609
FatI CATG 2 cut(s) 426, 686
FauNDI CATATG 1 cut(s) 999
FblI GTMKAC 1 cut(s) 632
Fnu4HI GCNGC 2 cut(s) 239, 754
FokI GGATG 4 cut(s) 790, 806, 848, 944
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 2 cut(s) 239, 754
FspBI CTAG 2 cut(s) 93, 245
FspI TGCGCA 1 cut(s) 986
GlaI GCGC 6 cut(s) 237, 537, 587, 589, 752, 986
GluI GCNGC 2 cut(s) 239, 754
GsuI CTGGAG 2 cut(s) 538, 926
HaeIII GGCC 5 cut(s) 173, 485, 516, 692, 728
HapII CCGG 4 cut(s) 46, 404, 618, 650
HgaI GACGC 4 cut(s) 116, 268, 563, 909
HhaI GCGC 6 cut(s) 238, 538, 588, 590, 753, 987
Hin1I GRCGYC 1 cut(s) 920
Hin1II CATG 2 cut(s) 430, 690
Hin6I GCGC 6 cut(s) 236, 536, 586, 588, 751, 985
HinP1I GCGC 6 cut(s) 236, 536, 586, 588, 751, 985
HincII GTYRAC 1 cut(s) 633
HindII GTYRAC 1 cut(s) 633
HinfI GANTC 8 cut(s) 193, 251, 409, 520, 621, 695, 713, 744
HpaII CCGG 4 cut(s) 46, 404, 618, 650
HphI GGTGA 4 cut(s) 343, 356, 398, 613
Hpy166II GTNNAC 4 cut(s) 633, 684, 862, 961
Hpy188I TCNGA 7 cut(s) 202, 223, 439, 500, 507, 668, 931
Hpy188III TCNNGA 2 cut(s) 245, 650
Hpy8I GTNNAC 4 cut(s) 633, 684, 862, 961
Hpy99I CGWCG 3 cut(s) 403, 579, 925
HpyCH4III ACNGT 2 cut(s) 583, 607
HpyCH4IV ACGT 3 cut(s) 541, 798, 808
HpyCH4V TGCA 1 cut(s) 300
HpyF10VI GCNNNNNNNGC 8 cut(s) 339, 400, 423, 522, 623, 725, 748, 851
HpyF3I CTNAG 2 cut(s) 54, 527
HpySE526I ACGT 3 cut(s) 541, 798, 808
Hsp92I GRCGYC 1 cut(s) 920
Hsp92II CATG 2 cut(s) 430, 690
HspAI GCGC 6 cut(s) 236, 536, 586, 588, 751, 985
Kpn2I TCCGGA 1 cut(s) 649
Kzo9I GATC 8 cut(s) 82, 223, 318, 419, 445, 502, 549, 644
LguI GCTCTTC 1 cut(s) 384
LmnI GCTCC 5 cut(s) 101, 120, 342, 399, 477
MaeI CTAG 2 cut(s) 93, 245
MaeII ACGT 3 cut(s) 541, 798, 808
MaeIII GTNAC 3 cut(s) 30, 601, 950
MalI GATC 8 cut(s) 84, 225, 320, 421, 447, 504, 551, 646
MboI GATC 8 cut(s) 82, 223, 318, 419, 445, 502, 549, 644
MboII GAAGA 4 cut(s) 353, 401, 450, 797
MflI RGATCY 4 cut(s) 82, 502, 549, 644
MhlI GDGCHC 2 cut(s) 117, 396
MluCI AATT 2 cut(s) 76, 1045
MlyI GAGTC 3 cut(s) 187, 514, 704
MmeI TCCRAC 4 cut(s) 45, 762, 771, 918
MnlI CCTC 8 cut(s) 224, 252, 305, 682, 811, 862, 873, 883
MroI TCCGGA 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 1 cut(s) 813
MspI CCGG 4 cut(s) 46, 404, 618, 650
MspR9I CCNGG 3 cut(s) 47, 160, 842
MvaI CCWGG 2 cut(s) 160, 842
MvnI CGCG 7 cut(s) 236, 450, 536, 588, 590, 626, 753
MwoI GCNNNNNNNGC 8 cut(s) 339, 400, 423, 522, 623, 725, 748, 851
NciI CCSGG 1 cut(s) 47
NdeI CATATG 1 cut(s) 999
NdeII GATC 8 cut(s) 82, 223, 318, 419, 445, 502, 549, 644
NlaIII CATG 2 cut(s) 430, 690
NlaIV GGNNCC 1 cut(s) 665
NmeAIII GCCGAG 5 cut(s) 340, 351, 653, 718, 754
NmuCI GTSAC 2 cut(s) 30, 601
NsbI TGCGCA 1 cut(s) 986
NspI RCATGY 1 cut(s) 430
PauI GCGCGC 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 384
PcsI WCGNNNNNNNCGW 1 cut(s) 960
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 5 cut(s) 251, 409, 621, 713, 744
PkrI GCNGC 2 cut(s) 240, 755
Ple19I CGATCG 1 cut(s) 448
PleI GAGTC 3 cut(s) 187, 514, 703
PmaCI CACGTG 2 cut(s) 542, 809
PmlI CACGTG 2 cut(s) 542, 809
PpsI GAGTC 3 cut(s) 187, 514, 703
Ppu21I YACGTR 2 cut(s) 542, 809
Psp124BI GAGCTC 1 cut(s) 396
Psp6I CCWGG 2 cut(s) 158, 840
PspCI CACGTG 2 cut(s) 542, 809
PspGI CCWGG 2 cut(s) 158, 840
PspN4I GGNNCC 1 cut(s) 665
PspPI GGNCC 7 cut(s) 137, 271, 292, 514, 664, 690, 726
PsuI RGATCY 4 cut(s) 82, 502, 549, 644
PteI GCGCGC 1 cut(s) 586
PvuI CGATCG 1 cut(s) 448
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
Rsr2I CGGWCCG 1 cut(s) 137
RsrII CGGWCCG 1 cut(s) 137
SacI GAGCTC 1 cut(s) 396
SalI GTCGAC 1 cut(s) 631
SapI GCTCTTC 1 cut(s) 384
SaqAI TTAA 1 cut(s) 813
SatI GCNGC 2 cut(s) 239, 754
Sau3AI GATC 8 cut(s) 82, 223, 318, 419, 445, 502, 549, 644
Sau96I GGNCC 7 cut(s) 137, 271, 292, 514, 664, 690, 726
SchI GAGTC 3 cut(s) 187, 514, 704
ScrFI CCNGG 3 cut(s) 47, 160, 842
SduI GDGCHC 2 cut(s) 117, 396
SinI GGWCC 4 cut(s) 137, 271, 292, 664
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 76, 1045
SspMI CTAG 2 cut(s) 93, 245
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 3 cut(s) 45, 158, 840
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 583, 607
TaiI ACGT 3 cut(s) 544, 801, 811
TaqI TCGA 5 cut(s) 102, 321, 444, 577, 632
TaqII GACCGA 2 cut(s) 125, 393
TasI AATT 2 cut(s) 76, 1045
TauI GCSGC 2 cut(s) 241, 756
TfiI GAWTC 5 cut(s) 251, 409, 621, 713, 744
Tru1I TTAA 1 cut(s) 813
Tru9I TTAA 1 cut(s) 813
TscAI CASTG 1 cut(s) 588
TseFI GTSAC 2 cut(s) 30, 601
Tsp45I GTSAC 2 cut(s) 30, 601
TspDTI ATGAA 2 cut(s) 335, 601
TspGWI ACGGA 6 cut(s) 26, 55, 60, 657, 969, 1019
TspRI CASTG 1 cut(s) 588
VpaK11BI GGWCC 4 cut(s) 137, 271, 292, 664
XbaI TCTAGA 1 cut(s) 244
XceI RCATGY 1 cut(s) 430
XmiI GTMKAC 1 cut(s) 632
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 2 cut(s) 93, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.