FvH4_4g37200

Mur ligase family, glutamate ligase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018433
Physical Location & Seq
Reverse (-)
33774404 .. 33775206
803 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g37200.t1

Sequence Viewer

Length: 675 bp
ATGGGAATCGGAACAACCGAATTGATGGAGATGGAGATGAGTGGCAGGGGAGAGCTATTGGAGCTTGCGAGGATGGCTGAGCCGAATATAAGGGGAGGTTCACACTTCGACAACTTCTTCAGTTTAGAGCAATTTGCTATGGCCAAAGGGGAGAGATTCTGGAGGAGGCAAAGCCCCGAGATGCGTGTGTTTCTCAATGCTGATGATCTCCCTCTACCATTTGGGGTTCAAAAGGGTGGAATTTTTTATTCAGGTCTGCATTTGGCTCTCAATGCATGTGCAGCAGCGGCAGTTGCAACTCGTTTAGGAGTTTCTCTTTCTCAAGTTGGAATTTTTCTATCAGCATTTTCTCCCCTCAAAATGAGGTCAGAGCTTGAATTTGCTAGAAATGATGTCAAGATAGTCAATGATGCTCACAATGCCAATCCTGTGAGCACCAGAGCTGCCATTGACTCATTGCAGAGTATTTATTGCAAGGGCAAGAAAATTTCATTACTGGGCGACATGTTAGAACTGGGTGCGATTGAGTTGGAGTCTCACAAGATGATTCTTGAGCGTTGTCGTAGTGCTCATGATATTGATTTAATTGGCCTAGTTGGGAAAAGGTTTTGCACAGCAGCCAAGGATGTGGATTTGGTTGAAGAGTGCAATATTGTGTCCGCTCTTGAGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase_C Mur_ligase_M

Protein Analysis

225

Amino Acids

24.55

Weight (kDa)

5.6

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mur_ligase_C PF02875 128 - 205 2.4e-07 Mur ligase, glutamate ligase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018433)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37200
malus_domestica MD13G1002800.v1.1
prunus_persica Prupe.1G349600_v2.0.a1
rosa_laevigata RLG00000005615
rosa_rugosa Rorug04G0376600
rosa_samantha Rh4AG438200 Rh4BG447200 Rh4CG464400 Rh4DG445900
rosa_wichuraiana Rw4G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 662
AciI CCGC 2 cut(s) 287, 660
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 4 cut(s) 240, 330, 377, 486
AcuI CTGAAG 1 cut(s) 103
AflIII ACRYGT 1 cut(s) 504
AgsI TTSAA 3 cut(s) 230, 377, 641
AluBI AGCT 4 cut(s) 55, 64, 373, 443
AluI AGCT 4 cut(s) 55, 64, 373, 443
Alw21I GWGCWC 2 cut(s) 437, 571
Alw26I GTCTC 1 cut(s) 540
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 2 cut(s) 141, 589
ApeKI GCWGC 4 cut(s) 281, 284, 443, 617
ApoI RAATTY 4 cut(s) 240, 330, 377, 486
AvaI CYCGRG 1 cut(s) 176
BalI TGGCCA 1 cut(s) 143
Bbv12I GWGCWC 2 cut(s) 437, 571
BbvI GCAGC 4 cut(s) 293, 296, 430, 629
BccI CCATC 3 cut(s) 19, 25, 67
BcoDI GTCTC 1 cut(s) 540
BfaI CTAG 2 cut(s) 384, 593
BisI GCNGC 5 cut(s) 282, 285, 288, 444, 618
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 5 cut(s) 283, 286, 289, 445, 619
BmeT110I CYCGRG 1 cut(s) 176
BmrI ACTGGG 2 cut(s) 506, 524
BmsI GCATC 2 cut(s) 171, 400
BmuI ACTGGG 2 cut(s) 506, 524
BpmI CTGGAG 1 cut(s) 181
Bpu1102I GCTNAGC 1 cut(s) 78
BpuEI CTTGAG 2 cut(s) 306, 572
BsaJI CCNNGG 1 cut(s) 621
Bse1I ACTGG 2 cut(s) 501, 519
Bse3DI GCAATG 1 cut(s) 455
BseDI CCNNGG 1 cut(s) 621
BseGI GGATG 2 cut(s) 78, 631
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 2 cut(s) 501, 519
BseRI GAGGAG 1 cut(s) 178
BseXI GCAGC 4 cut(s) 293, 296, 430, 629
BsgI GTGCAG 1 cut(s) 300
BshFI GGCC 2 cut(s) 143, 591
BsiHKAI GWGCWC 2 cut(s) 437, 571
BsiHKCI CYCGRG 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 540
BsnI GGCC 2 cut(s) 143, 591
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 2 cut(s) 437, 571
Bsp143I GATC 1 cut(s) 205
Bsp1720I GCTNAGC 1 cut(s) 78
BspACI CCGC 2 cut(s) 287, 660
BspANI GGCC 2 cut(s) 143, 591
BspCNI CTCAG 1 cut(s) 70
BspHI TCATGA 1 cut(s) 571
BsrBI CCGCTC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 455
BsrI ACTGG 2 cut(s) 501, 519
BssECI CCNNGG 1 cut(s) 621
BssMI GATC 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 621
Bst6I CTCTTC 1 cut(s) 636
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 78
BstF5I GGATG 2 cut(s) 78, 631
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 540
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 8 cut(s) 61, 74, 272, 281, 287, 293, 419, 668
BstNSI RCATGY 2 cut(s) 279, 508
BstV1I GCAGC 4 cut(s) 293, 296, 430, 629
BstXI CCANNNNNNTGG 1 cut(s) 628
BsuRI GGCC 2 cut(s) 143, 591
BtsCI GGATG 2 cut(s) 78, 631
Cac8I GCNNGC 1 cut(s) 66
CciI TCATGA 1 cut(s) 571
CviAII CATG 3 cut(s) 276, 505, 572
DdeI CTNAG 1 cut(s) 78
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
Eco130I CCWWGG 1 cut(s) 621
Eco57I CTGAAG 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 621
EcoT22I ATGCAT 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 3 cut(s) 279, 508, 575
FaiI YATR 6 cut(s) 89, 140, 277, 506, 573, 673
FatI CATG 3 cut(s) 275, 504, 571
Fnu4HI GCNGC 5 cut(s) 282, 285, 288, 444, 618
FokI GGATG 2 cut(s) 85, 638
Fsp4HI GCNGC 5 cut(s) 282, 285, 288, 444, 618
FspBI CTAG 2 cut(s) 384, 593
GluI GCNGC 5 cut(s) 282, 285, 288, 444, 618
GsuI CTGGAG 1 cut(s) 181
HaeIII GGCC 2 cut(s) 143, 591
Hin1II CATG 3 cut(s) 279, 508, 575
HinfI GANTC 5 cut(s) 6, 156, 452, 533, 547
Hpy166II GTNNAC 1 cut(s) 101
Hpy188I TCNGA 2 cut(s) 11, 370
Hpy188III TCNNGA 5 cut(s) 160, 397, 551, 572, 665
Hpy8I GTNNAC 1 cut(s) 101
HpyCH4V TGCA 8 cut(s) 259, 275, 281, 296, 460, 474, 612, 648
HpyF10VI GCNNNNNNNGC 8 cut(s) 61, 74, 272, 281, 287, 293, 419, 668
HpyF3I CTNAG 1 cut(s) 78
Hsp92II CATG 3 cut(s) 279, 508, 575
Kzo9I GATC 1 cut(s) 205
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 7 cut(s) 31, 145, 237, 441, 451, 482, 500
Lsp1109I GCAGC 4 cut(s) 293, 296, 430, 629
LweI GCATC 2 cut(s) 171, 400
MaeI CTAG 2 cut(s) 384, 593
MalI GATC 1 cut(s) 207
MbiI CCGCTC 1 cut(s) 662
MboI GATC 1 cut(s) 205
MboII GAAGA 2 cut(s) 109, 653
MhlI GDGCHC 2 cut(s) 437, 571
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 7 cut(s) 20, 131, 240, 330, 377, 486, 585
MluNI TGGCCA 1 cut(s) 143
MlyI GAGTC 2 cut(s) 446, 542
MmeI TCCRAC 2 cut(s) 307, 510
MnlI CCTC 8 cut(s) 63, 89, 156, 159, 222, 357, 365, 661
Mox20I TGGCCA 1 cut(s) 143
Mph1103I ATGCAT 1 cut(s) 277
MscI TGGCCA 1 cut(s) 143
MseI TTAA 1 cut(s) 584
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 287
MwoI GCNNNNNNNGC 8 cut(s) 61, 74, 272, 281, 287, 293, 419, 668
NdeII GATC 1 cut(s) 205
NlaIII CATG 3 cut(s) 279, 508, 575
NsiI ATGCAT 1 cut(s) 277
NspI RCATGY 2 cut(s) 279, 508
PagI TCATGA 1 cut(s) 571
PciI ACATGT 1 cut(s) 504
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PfeI GAWTC 3 cut(s) 6, 156, 547
PkrI GCNGC 5 cut(s) 283, 286, 289, 445, 619
PleI GAGTC 2 cut(s) 446, 541
PpsI GAGTC 2 cut(s) 446, 541
PscI ACATGT 1 cut(s) 504
SaqAI TTAA 1 cut(s) 584
SatI GCNGC 5 cut(s) 282, 285, 288, 444, 618
Sau3AI GATC 1 cut(s) 205
SchI GAGTC 2 cut(s) 446, 542
SduI GDGCHC 2 cut(s) 437, 571
SetI ASST 8 cut(s) 57, 66, 100, 256, 368, 375, 445, 608
SfaNI GCATC 2 cut(s) 171, 400
SmlI CTYRAG 3 cut(s) 321, 551, 665
SmoI CTYRAG 3 cut(s) 321, 551, 665
Sse9I AATT 7 cut(s) 20, 131, 240, 330, 377, 486, 585
SsiI CCGC 2 cut(s) 287, 660
SspI AATATT 1 cut(s) 652
SspMI CTAG 2 cut(s) 384, 593
StyI CCWWGG 1 cut(s) 621
TaqI TCGA 1 cut(s) 108
TasI AATT 7 cut(s) 20, 131, 240, 330, 377, 486, 585
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 3 cut(s) 6, 156, 547
Tru1I TTAA 1 cut(s) 584
Tru9I TTAA 1 cut(s) 584
TseI GCWGC 4 cut(s) 281, 284, 443, 617
TspDTI ATGAA 1 cut(s) 480
XapI RAATTY 4 cut(s) 240, 330, 377, 486
XceI RCATGY 2 cut(s) 279, 508
XspI CTAG 2 cut(s) 384, 593
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.