Rh4AG438200

Mur ligase family, glutamate ligase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0018433
Physical Location & Seq
Reverse (-)
73032417 .. 73033459
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG438200.1

Sequence Viewer

Length: 444 bp
ATGGCCAAAGGGAAGATTCTCCAGGAGGCAAAGCCCGGAGACGTGTGTGTTCTCCATTCTGATGATCCATTAGTTAGGAGCCTCCTCCCTCTACCATTTGGGGTTCAAAAGGTGCGCCCTTTTCAGATGCCCTTCCCTAGCTTGGTAGAATTTGTGATACCCAGTCCAGGTCTGCATTTGGCTCTCAATGCTTGTGCAGCAGCGGCAGTTGCAACTCTTTTAGGAGTTTCTCTTTCTCAAGTTGGAAGTTGTCTATCAGCATTTACTCCCGTCAAAATGAGGTCAGAGCTTGAAGTTGCTAGAAATGGAGTCGAGATAGTCAATGATGTTTACAATGCCAATCCTGTGAGCTCCAGAGCTGCCATTGACTTATTGAAGAGTACTATTGATTGCAAGGGCCAAAAAATTGCCATACTTGGTGACATGTTAGAACTCGGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase_C Mur_ligase_M

Protein Analysis

147

Amino Acids

15.49

Weight (kDa)

6.71

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mur_ligase_C PF02875 94 - 146 2.8e-06 Mur ligase, glutamate ligase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018433)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37200
malus_domestica MD13G1002800.v1.1
prunus_persica Prupe.1G349600_v2.0.a1
rosa_laevigata RLG00000005615
rosa_rugosa Rorug04G0376600
rosa_samantha Rh4AG438200 Rh4BG447200 Rh4CG464400 Rh4DG445900
rosa_wichuraiana Rw4G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 203
AclWI GGATC 1 cut(s) 59
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 149
AfaI GTAC 1 cut(s) 382
AfiI CCNNNNNNNGG 2 cut(s) 142, 167
AflIII ACRYGT 2 cut(s) 42, 423
AgsI TTSAA 3 cut(s) 107, 293, 376
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 2 cut(s) 21, 166
AluBI AGCT 4 cut(s) 141, 289, 351, 359
AluI AGCT 4 cut(s) 141, 289, 351, 359
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 33
AlwI GGATC 1 cut(s) 59
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 3 cut(s) 3, 397, 438
ApeKI GCWGC 3 cut(s) 197, 200, 359
ApoI RAATTY 1 cut(s) 149
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 2 cut(s) 397, 438
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 434
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 3 cut(s) 209, 212, 346
BciT130I CCWGG 2 cut(s) 23, 168
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 33
BfaI CTAG 3 cut(s) 138, 300, 442
BisI GCNGC 4 cut(s) 198, 201, 204, 360
BlsI GCNGC 4 cut(s) 199, 202, 205, 361
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 3 cut(s) 23, 36, 168
BmeT110I CYCGRG 1 cut(s) 434
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 397, 438
BmiI GGNNCC 2 cut(s) 80, 439
BmrFI CCNGG 3 cut(s) 23, 36, 168
BmrI ACTGGG 1 cut(s) 156
BmsI GCATC 1 cut(s) 117
BmuI ACTGGG 1 cut(s) 156
BpmI CTGGAG 2 cut(s) 5, 337
BpuEI CTTGAG 1 cut(s) 222
BpuMI CCSGG 1 cut(s) 36
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 167
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 2 cut(s) 23, 168
BseLI CCNNNNNNNGG 2 cut(s) 142, 167
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 1 cut(s) 74
BseXI GCAGC 3 cut(s) 209, 212, 346
BsgI GTGCAG 1 cut(s) 216
BshFI GGCC 3 cut(s) 5, 399, 440
BsiHKAI GWGCWC 1 cut(s) 353
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 142, 167
BsmAI GTCTC 1 cut(s) 33
BsmBI CGTCTC 1 cut(s) 33
BsnI GGCC 3 cut(s) 5, 399, 440
BsoBI CYCGRG 1 cut(s) 434
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 1 cut(s) 64
BspACI CCGC 1 cut(s) 203
BspANI GGCC 3 cut(s) 5, 399, 440
BspLI GGNNCC 2 cut(s) 80, 439
BspPI GGATC 1 cut(s) 59
BsrI ACTGG 1 cut(s) 162
BssMI GATC 1 cut(s) 64
Bst2UI CCWGG 2 cut(s) 23, 168
Bst6I CTCTTC 1 cut(s) 371
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 1 cut(s) 67
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 1 cut(s) 64
BstMWI GCNNNNNNNGC 4 cut(s) 188, 197, 203, 209
BstNI CCWGG 2 cut(s) 23, 168
BstNSI RCATGY 1 cut(s) 427
BstSCI CCNGG 3 cut(s) 21, 34, 166
BstV1I GCAGC 3 cut(s) 209, 212, 346
BsuRI GGCC 3 cut(s) 5, 399, 440
BtrI CACGTC 1 cut(s) 43
CfoI GCGC 1 cut(s) 117
Cfr13I GGNCC 2 cut(s) 397, 438
Csp6I GTAC 1 cut(s) 381
CviAII CATG 1 cut(s) 424
CviQI GTAC 1 cut(s) 381
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 371
EarI CTCTTC 1 cut(s) 371
Ecl136II GAGCTC 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 434
EcoICRI GAGCTC 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 438
EcoRII CCWGG 2 cut(s) 21, 166
EcoT38I GRGCYC 1 cut(s) 353
Esp3I CGTCTC 1 cut(s) 33
FaeI CATG 1 cut(s) 427
FaiI YATR 2 cut(s) 413, 425
FatI CATG 1 cut(s) 423
Fnu4HI GCNGC 4 cut(s) 198, 201, 204, 360
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 4 cut(s) 198, 201, 204, 360
FspBI CTAG 3 cut(s) 138, 300, 442
GlaI GCGC 1 cut(s) 116
GluI GCNGC 4 cut(s) 198, 201, 204, 360
GsuI CTGGAG 2 cut(s) 5, 337
HaeIII GGCC 3 cut(s) 5, 399, 440
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 117
Hin1II CATG 1 cut(s) 427
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 2 cut(s) 16, 309
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 61, 126, 286
Hpy188III TCNNGA 2 cut(s) 313, 354
Hpy8I GTNNAC 1 cut(s) 331
HpyAV CCTTC 1 cut(s) 142
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 4 cut(s) 175, 197, 212, 393
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 197, 203, 209
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 1 cut(s) 427
HspAI GCGC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 64
LmnI GCTCC 2 cut(s) 78, 356
LpnPI CCDG 8 cut(s) 8, 35, 49, 153, 175, 180, 357, 367
Lsp1109I GCAGC 3 cut(s) 209, 212, 346
LweI GCATC 1 cut(s) 117
MaeI CTAG 3 cut(s) 138, 300, 442
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 419
MalI GATC 1 cut(s) 66
MboI GATC 1 cut(s) 64
MboII GAAGA 2 cut(s) 25, 388
MhlI GDGCHC 1 cut(s) 353
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 149, 405
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 318
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 5 cut(s) 19, 92, 95, 99, 273
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 60
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 3 cut(s) 23, 36, 168
MvaI CCWGG 2 cut(s) 23, 168
MwoI GCNNNNNNNGC 4 cut(s) 188, 197, 203, 209
NciI CCSGG 1 cut(s) 36
NdeII GATC 1 cut(s) 64
NlaIII CATG 1 cut(s) 427
NlaIV GGNNCC 2 cut(s) 80, 439
NmuCI GTSAC 1 cut(s) 419
NspI RCATGY 1 cut(s) 427
PciI ACATGT 1 cut(s) 423
PfeI GAWTC 1 cut(s) 16
PfoI TCCNGGA 1 cut(s) 21
PkrI GCNGC 4 cut(s) 199, 202, 205, 361
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
PscI ACATGT 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 353
Psp6I CCWGG 2 cut(s) 21, 166
PspGI CCWGG 2 cut(s) 21, 166
PspN4I GGNNCC 2 cut(s) 80, 439
PspPI GGNCC 2 cut(s) 397, 438
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
RseI CAYNNNNRTG 1 cut(s) 60
SacI GAGCTC 1 cut(s) 353
SatI GCNGC 4 cut(s) 198, 201, 204, 360
Sau3AI GATC 1 cut(s) 64
Sau96I GGNCC 2 cut(s) 397, 438
ScaI AGTACT 1 cut(s) 382
SchI GAGTC 1 cut(s) 318
ScrFI CCNGG 3 cut(s) 23, 36, 168
SduI GDGCHC 1 cut(s) 353
SetI ASST 8 cut(s) 45, 114, 143, 172, 284, 291, 353, 361
SfaNI GCATC 1 cut(s) 117
SmiMI CAYNNNNRTG 1 cut(s) 60
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
Sse9I AATT 2 cut(s) 149, 405
SsiI CCGC 1 cut(s) 203
SspMI CTAG 3 cut(s) 138, 300, 442
SstI GAGCTC 1 cut(s) 353
StyD4I CCNGG 3 cut(s) 21, 34, 166
TaiI ACGT 1 cut(s) 45
TaqI TCGA 1 cut(s) 312
TasI AATT 2 cut(s) 149, 405
TatI WGTACW 1 cut(s) 380
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 1 cut(s) 16
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 3 cut(s) 197, 200, 359
Tsp45I GTSAC 1 cut(s) 419
XapI RAATTY 1 cut(s) 149
XceI RCATGY 1 cut(s) 427
XspI CTAG 3 cut(s) 138, 300, 442
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.