Rw4G037480

Mur ligase family, glutamate ligase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018433
Physical Location & Seq
Reverse (-)
64060280 .. 64061929
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037480.1

Sequence Viewer

Length: 909 bp
ATGCTTCTTCGCCATTTCCGGCCAAAACTTGGACGCCCACGATTGCAATTATCCCTAGATCAAGTCTGCGAGAACTGGGACGTTGAAACCGAGGCTGATACTCTGGTTTCACTGACCAACATGGCCAGCTATGGAGAGATACTGGAGCTTGCGAGGACCGGCGCTTCACACTTTGACAACTTCTCCAGTGCTGCTATGGCCAAAGGGGAGATTCTCCAGGTCCAGGAGGCAAAGCCCGGAGACGTGTGTGTTCTCCATCATGATGATCCATTAGTTATGAGCCTCCTCCCTCTACCATTTGGGGTTCAAAAGGTGCTCTTTGGTCAGACGGCGGGTGTGATGTTCGTTTGGTTGAGTGCGAAAGCACACACGGAGGTCTTGGAGTATCATACAGAGATGGTAGAATTTGTGATACCCAGTCCAGGTCTGCATTTGGCTCTCAATGCTTGTGCAGCAGCGGCAGTTGCAACTCTTTTAGGAGTTTCTCTTTCTCAAGTTGGAATTTGTCTATCAGCATTTACTCCCGTCAAAATGAGGTCAGAGCTTGAAGTTGCTAGAAATGGAGTCAAGATAGTCAGTGATGTTTACAATGCCAATCCTGTGAGATCCAGAGCTGCCATTGACTTATTGAAGAGTACTATTGATTGCAAGGGCCAAAAATTGCCATACTTGGTGACATGTTCTGCACGTACAGCAGCCAAGGATATGAATTTGCTTGAAAAGTTTAATATTGTGTGCTCTCTTGAGCCAGAAACTTTTGCAGAGGAAATTGTGAAGAGGATAAATTGTAATGATGTTATTTTTGTAAAGGGTAGTCGAGGGATGCGAATGGAGAAAGTTGTAAATGCAATCAAGCAATGGAAATTTGAGATACACATTTCTCAGCTGTTGATGATTTTCCTGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase_C Mur_ligase_M

Protein Analysis

302

Amino Acids

33.39

Weight (kDa)

6.46

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018433)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37200
malus_domestica MD13G1002800.v1.1
prunus_persica Prupe.1G349600_v2.0.a1
rosa_laevigata RLG00000005615
rosa_rugosa Rorug04G0376600
rosa_samantha Rh4AG438200 Rh4BG447200 Rh4CG464400 Rh4DG445900
rosa_wichuraiana Rw4G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 29
AciI CCGC 2 cut(s) 332, 458
AclWI GGATC 2 cut(s) 260, 600
AcoI YGGCCR 3 cut(s) 20, 123, 198
AcsI RAATTY 4 cut(s) 404, 501, 709, 863
AcyI GRCGYC 1 cut(s) 34
AfaI GTAC 2 cut(s) 637, 691
AfiI CCNNNNNNNGG 3 cut(s) 29, 223, 422
AflIII ACRYGT 2 cut(s) 243, 677
AgsI TTSAA 5 cut(s) 86, 308, 548, 631, 719
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 3 cut(s) 216, 222, 421
AluBI AGCT 5 cut(s) 129, 148, 544, 614, 886
AluI AGCT 5 cut(s) 129, 148, 544, 614, 886
Alw21I GWGCWC 2 cut(s) 318, 740
Alw26I GTCTC 1 cut(s) 234
AlwI GGATC 2 cut(s) 260, 600
AoxI GGCC 4 cut(s) 20, 123, 198, 652
ApeKI GCWGC 5 cut(s) 191, 452, 455, 614, 695
ApoI RAATTY 4 cut(s) 404, 501, 709, 863
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 3 cut(s) 156, 220, 652
AsuC2I CCSGG 1 cut(s) 237
AsuHPI GGTGA 1 cut(s) 685
AvaII GGWCC 2 cut(s) 156, 220
BaeI ACNNNNGTAYC 2 cut(s) 90, 123
BalI TGGCCA 2 cut(s) 125, 200
Bbv12I GWGCWC 2 cut(s) 318, 740
BbvI GCAGC 5 cut(s) 178, 464, 467, 601, 707
BccI CCATC 2 cut(s) 264, 391
BceAI ACGGC 1 cut(s) 345
BciT130I CCWGG 3 cut(s) 218, 224, 423
BcnI CCSGG 1 cut(s) 237
BcoDI GTCTC 1 cut(s) 234
BfaI CTAG 2 cut(s) 56, 555
BfoI RGCGCY 1 cut(s) 165
BisI GCNGC 6 cut(s) 192, 453, 456, 459, 615, 696
BlsI GCNGC 6 cut(s) 193, 454, 457, 460, 616, 697
BmcAI AGTACT 1 cut(s) 637
Bme1390I CCNGG 4 cut(s) 218, 224, 237, 423
Bme18I GGWCC 2 cut(s) 156, 220
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 3 cut(s) 156, 220, 652
BmrFI CCNGG 4 cut(s) 218, 224, 237, 423
BmrI ACTGGG 2 cut(s) 85, 411
BmsI GCATC 1 cut(s) 813
BmuI ACTGGG 2 cut(s) 85, 411
BpmI CTGGAG 3 cut(s) 164, 169, 200
BpuEI CTTGAG 2 cut(s) 477, 764
BpuMI CCSGG 1 cut(s) 237
BsaAI YACGTR 1 cut(s) 689
BsaHI GRCGYC 1 cut(s) 34
BsaJI CCNNGG 2 cut(s) 90, 699
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 223, 422
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 4 cut(s) 80, 147, 186, 417
Bse3DI GCAATG 1 cut(s) 863
BseBI CCWGG 3 cut(s) 218, 224, 423
BseDI CCNNGG 2 cut(s) 90, 699
BseGI GGATG 1 cut(s) 828
BseLI CCNNNNNNNGG 3 cut(s) 29, 223, 422
BseMI GCAATG 1 cut(s) 863
BseMII CTCAG 1 cut(s) 896
BseNI ACTGG 4 cut(s) 80, 147, 186, 417
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 5 cut(s) 178, 464, 467, 601, 707
BsgI GTGCAG 2 cut(s) 471, 669
BshFI GGCC 4 cut(s) 22, 125, 200, 654
BsiHKAI GWGCWC 2 cut(s) 318, 740
BsiSI CCGG 3 cut(s) 19, 159, 237
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 3 cut(s) 29, 223, 422
BsmAI GTCTC 1 cut(s) 234
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 4 cut(s) 22, 125, 200, 654
Bsp1286I GDGCHC 2 cut(s) 318, 740
Bsp143I GATC 3 cut(s) 58, 265, 605
BspACI CCGC 2 cut(s) 332, 458
BspANI GGCC 4 cut(s) 22, 125, 200, 654
BspCNI CTCAG 1 cut(s) 895
BspHI TCATGA 1 cut(s) 259
BspPI GGATC 2 cut(s) 260, 600
BsrDI GCAATG 1 cut(s) 863
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 4 cut(s) 80, 147, 186, 417
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 2 cut(s) 90, 699
BssMI GATC 3 cut(s) 58, 265, 605
BssNI GRCGYC 1 cut(s) 34
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 3 cut(s) 218, 224, 423
Bst6I CTCTTC 2 cut(s) 626, 770
BstACI GRCGYC 1 cut(s) 34
BstBAI YACGTR 1 cut(s) 689
BstC8I GCNNGC 2 cut(s) 127, 150
BstDEI CTNAG 1 cut(s) 882
BstF5I GGATG 1 cut(s) 828
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 3 cut(s) 61, 268, 608
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 3 cut(s) 58, 265, 605
BstMWI GCNNNNNNNGC 6 cut(s) 197, 443, 452, 458, 464, 692
BstNI CCWGG 3 cut(s) 218, 224, 423
BstNSI RCATGY 1 cut(s) 681
BstSCI CCNGG 4 cut(s) 216, 222, 235, 421
BstV1I GCAGC 5 cut(s) 178, 464, 467, 601, 707
BstX2I RGATCY 1 cut(s) 605
BstYI RGATCY 1 cut(s) 605
BsuRI GGCC 4 cut(s) 22, 125, 200, 654
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 1 cut(s) 828
BtsIMutI CAGTG 3 cut(s) 110, 193, 583
Cac8I GCNNGC 2 cut(s) 127, 150
CciI TCATGA 1 cut(s) 259
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 3 cut(s) 156, 220, 652
CseI GACGC 1 cut(s) 42
Csp6I GTAC 2 cut(s) 636, 690
CviAII CATG 3 cut(s) 121, 260, 678
CviQI GTAC 2 cut(s) 636, 690
DdeI CTNAG 1 cut(s) 882
DpnI GATC 3 cut(s) 60, 267, 607
DpnII GATC 3 cut(s) 58, 265, 605
EaeI YGGCCR 3 cut(s) 20, 123, 198
Eam1104I CTCTTC 2 cut(s) 626, 770
EarI CTCTTC 2 cut(s) 626, 770
Eco130I CCWWGG 1 cut(s) 699
Eco47I GGWCC 2 cut(s) 156, 220
EcoRII CCWGG 3 cut(s) 216, 222, 421
EcoT14I CCWWGG 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 1 cut(s) 234
FaeI CATG 3 cut(s) 124, 263, 681
FaiI YATR 9 cut(s) 122, 132, 197, 261, 278, 390, 667, 679, 707
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FaqI GGGAC 1 cut(s) 92
FatI CATG 3 cut(s) 120, 259, 677
FauI CCCGC 1 cut(s) 325
Fnu4HI GCNGC 6 cut(s) 192, 453, 456, 459, 615, 696
FokI GGATG 1 cut(s) 835
Fsp4HI GCNGC 6 cut(s) 192, 453, 456, 459, 615, 696
FspBI CTAG 2 cut(s) 56, 555
GlaI GCGC 1 cut(s) 163
GluI GCNGC 6 cut(s) 192, 453, 456, 459, 615, 696
GsuI CTGGAG 3 cut(s) 164, 169, 200
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 4 cut(s) 22, 125, 200, 654
HapII CCGG 3 cut(s) 19, 159, 237
HgaI GACGC 1 cut(s) 42
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 34
Hin1II CATG 3 cut(s) 124, 263, 681
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HinfI GANTC 2 cut(s) 211, 564
HpaII CCGG 3 cut(s) 19, 159, 237
HphI GGTGA 1 cut(s) 685
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 2 cut(s) 327, 541
Hpy188III TCNNGA 5 cut(s) 260, 568, 609, 743, 901
Hpy8I GTNNAC 1 cut(s) 586
HpyCH4IV ACGT 3 cut(s) 81, 243, 688
HpyCH4V TGCA 8 cut(s) 46, 430, 452, 467, 648, 686, 761, 848
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 443, 452, 458, 464, 692
HpyF3I CTNAG 1 cut(s) 882
HpySE526I ACGT 3 cut(s) 81, 243, 688
Hsp92I GRCGYC 1 cut(s) 34
Hsp92II CATG 3 cut(s) 124, 263, 681
HspAI GCGC 1 cut(s) 162
Kzo9I GATC 3 cut(s) 58, 265, 605
LmnI GCTCC 1 cut(s) 145
Lsp1109I GCAGC 5 cut(s) 178, 464, 467, 601, 707
LweI GCATC 1 cut(s) 813
MaeI CTAG 2 cut(s) 56, 555
MaeII ACGT 3 cut(s) 81, 243, 688
MaeIII GTNAC 1 cut(s) 673
MalI GATC 3 cut(s) 60, 267, 607
MboI GATC 3 cut(s) 58, 265, 605
MboII GAAGA 2 cut(s) 643, 787
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 2 cut(s) 318, 740
MlsI TGGCCA 2 cut(s) 125, 200
MluCI AATT 8 cut(s) 47, 404, 501, 659, 709, 768, 784, 863
MluNI TGGCCA 2 cut(s) 125, 200
MlyI GAGTC 1 cut(s) 573
MmeI TCCRAC 1 cut(s) 478
Mox20I TGGCCA 2 cut(s) 125, 200
MscI TGGCCA 2 cut(s) 125, 200
MseI TTAA 1 cut(s) 726
MslI CAYNNNNRTG 1 cut(s) 261
Msp20I TGGCCA 2 cut(s) 125, 200
MspA1I CMGCKG 2 cut(s) 458, 886
MspI CCGG 3 cut(s) 19, 159, 237
MspR9I CCNGG 4 cut(s) 218, 224, 237, 423
MvaI CCWGG 3 cut(s) 218, 224, 423
MwoI GCNNNNNNNGC 6 cut(s) 197, 443, 452, 458, 464, 692
NciI CCSGG 1 cut(s) 237
NdeII GATC 3 cut(s) 58, 265, 605
NlaIII CATG 3 cut(s) 124, 263, 681
NmuCI GTSAC 1 cut(s) 673
NspI RCATGY 1 cut(s) 681
PagI TCATGA 1 cut(s) 259
PciI ACATGT 1 cut(s) 677
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 823
PfeI GAWTC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 29
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 6 cut(s) 193, 454, 457, 460, 616, 697
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
Ppu21I YACGTR 1 cut(s) 689
PscI ACATGT 1 cut(s) 677
Psp6I CCWGG 3 cut(s) 216, 222, 421
PspGI CCWGG 3 cut(s) 216, 222, 421
PspPI GGNCC 3 cut(s) 156, 220, 652
PsuI RGATCY 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 886
RsaI GTAC 2 cut(s) 637, 691
RsaNI GTAC 2 cut(s) 636, 690
RseI CAYNNNNRTG 1 cut(s) 261
SaqAI TTAA 1 cut(s) 726
SatI GCNGC 6 cut(s) 192, 453, 456, 459, 615, 696
Sau3AI GATC 3 cut(s) 58, 265, 605
Sau96I GGNCC 3 cut(s) 156, 220, 652
ScaI AGTACT 1 cut(s) 637
SchI GAGTC 1 cut(s) 573
ScrFI CCNGG 4 cut(s) 218, 224, 237, 423
SduI GDGCHC 2 cut(s) 318, 740
SfaNI GCATC 1 cut(s) 813
SinI GGWCC 2 cut(s) 156, 220
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 492, 743
SmoI CTYRAG 2 cut(s) 492, 743
Sse9I AATT 8 cut(s) 47, 404, 501, 659, 709, 768, 784, 863
SsiI CCGC 2 cut(s) 332, 458
SspI AATATT 1 cut(s) 730
SspMI CTAG 2 cut(s) 56, 555
StyD4I CCNGG 4 cut(s) 216, 222, 235, 421
StyI CCWWGG 1 cut(s) 699
TaiI ACGT 3 cut(s) 84, 246, 691
TaqI TCGA 1 cut(s) 817
TasI AATT 8 cut(s) 47, 404, 501, 659, 709, 768, 784, 863
TatI WGTACW 1 cut(s) 635
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 1 cut(s) 726
Tru9I TTAA 1 cut(s) 726
TscAI CASTG 3 cut(s) 117, 193, 583
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 5 cut(s) 191, 452, 455, 614, 695
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 1 cut(s) 722
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 3 cut(s) 117, 193, 583
Van91I CCANNNNNTGG 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 156, 220
XapI RAATTY 4 cut(s) 404, 501, 709, 863
XceI RCATGY 1 cut(s) 681
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XspI CTAG 2 cut(s) 56, 555
ZrmI AGTACT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.