Rh4DG445900

Mur ligase family, glutamate ligase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018433
Physical Location & Seq
Reverse (-)
64556127 .. 64556744
618 bp
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UTR
Exon/CDS
Intron
Rh4DG445900.1

Sequence Viewer

Length: 399 bp
ATGTTGGTTTGGTTGCGTGCGAAAGCACACACGGAGGTCTTGGAGTATCATACAGAGATGGTAGAATTTGTGATACCCAGTCCAGGTCTGCATTTGGCTCTCAATGCTTGTGCAGCAGCGGCAGTTGCAACTCTTTTAGGAGTTTCTCTTTCTCAAGTTGGAAGTTGTCTATCAGCATTTACTCCCGTCAAAATGAGGTCAGAGCTTGAAGTTGCTAGAAATGGAGTCAAGATAGTCAATGATGTTTACAATGCCAATCCTGTGAGCTCCAGAGCTGCCATTGACTTATTGAAGAGTACTATTGATTGCAAGGGCCAAAAAATTGCCATACTTGGTGACATGTTAGAACTCGGGCCTAGTTGGGAAGAGGTTCTGCACGTACAGCAGCCAAGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase_C Mur_ligase_M

Protein Analysis

132

Amino Acids

14.28

Weight (kDa)

6.05

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mur_ligase_C PF02875 66 - 120 2e-07 Mur ligase, glutamate ligase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018433)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37200
malus_domestica MD13G1002800.v1.1
prunus_persica Prupe.1G349600_v2.0.a1
rosa_laevigata RLG00000005615
rosa_rugosa Rorug04G0376600
rosa_samantha Rh4AG438200 Rh4BG447200 Rh4CG464400 Rh4DG445900
rosa_wichuraiana Rw4G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 119
AcsI RAATTY 1 cut(s) 65
AfaI GTAC 2 cut(s) 298, 381
AfiI CCNNNNNNNGG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 339
AgsI TTSAA 2 cut(s) 209, 292
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 3 cut(s) 205, 267, 275
AluI AGCT 3 cut(s) 205, 267, 275
Alw21I GWGCWC 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 2 cut(s) 313, 353
ApeKI GCWGC 4 cut(s) 113, 116, 275, 385
ApoI RAATTY 1 cut(s) 65
Asp700I GAANNNNTTC 1 cut(s) 369
AspS9I GGNCC 2 cut(s) 313, 353
AsuHPI GGTGA 1 cut(s) 347
AvaI CYCGRG 1 cut(s) 350
BanII GRGCYC 1 cut(s) 269
Bbv12I GWGCWC 1 cut(s) 269
BbvI GCAGC 3 cut(s) 125, 128, 262
BccI CCATC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 84
BfaI CTAG 2 cut(s) 216, 357
BisI GCNGC 5 cut(s) 114, 117, 120, 276, 386
BlsI GCNGC 5 cut(s) 115, 118, 121, 277, 387
BmcAI AGTACT 1 cut(s) 298
Bme1390I CCNGG 1 cut(s) 84
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 2 cut(s) 313, 353
BmrFI CCNGG 1 cut(s) 84
BmrI ACTGGG 1 cut(s) 72
BmuI ACTGGG 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 253
BpuEI CTTGAG 1 cut(s) 138
BsaAI YACGTR 1 cut(s) 379
BsaJI CCNNGG 1 cut(s) 389
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 389
BseLI CCNNNNNNNGG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 78
BseXI GCAGC 3 cut(s) 125, 128, 262
BsgI GTGCAG 2 cut(s) 132, 359
BshFI GGCC 2 cut(s) 315, 355
BsiHKAI GWGCWC 1 cut(s) 269
BsiHKCI CYCGRG 1 cut(s) 350
BslI CCNNNNNNNGG 1 cut(s) 83
BsnI GGCC 2 cut(s) 315, 355
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 269
BspACI CCGC 1 cut(s) 119
BspANI GGCC 2 cut(s) 315, 355
BsrI ACTGG 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 389
BssT1I CCWWGG 1 cut(s) 389
Bst2UI CCWGG 1 cut(s) 84
Bst6I CTCTTC 2 cut(s) 287, 360
BstBAI YACGTR 1 cut(s) 379
BstC8I GCNNGC 1 cut(s) 18
BstMWI GCNNNNNNNGC 5 cut(s) 104, 113, 119, 125, 382
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 1 cut(s) 343
BstSCI CCNGG 1 cut(s) 82
BstV1I GCAGC 3 cut(s) 125, 128, 262
BsuRI GGCC 2 cut(s) 315, 355
Cac8I GCNNGC 1 cut(s) 18
Cfr13I GGNCC 2 cut(s) 313, 353
Csp6I GTAC 2 cut(s) 297, 380
CviAII CATG 1 cut(s) 340
CviJI RGCY 7 cut(s) 98, 205, 267, 275, 315, 355, 388
CviKI_1 RGCY 7 cut(s) 98, 205, 267, 275, 315, 355, 388
CviQI GTAC 2 cut(s) 297, 380
Eam1104I CTCTTC 2 cut(s) 287, 360
EarI CTCTTC 2 cut(s) 287, 360
Ecl136II GAGCTC 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 389
Eco24I GRGCYC 1 cut(s) 269
Eco53kI GAGCTC 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 350
EcoICRI GAGCTC 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 389
EcoT38I GRGCYC 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 389
FaeI CATG 1 cut(s) 343
FaiI YATR 4 cut(s) 51, 329, 341, 397
FatI CATG 1 cut(s) 339
Fnu4HI GCNGC 5 cut(s) 114, 117, 120, 276, 386
FriOI GRGCYC 1 cut(s) 269
Fsp4HI GCNGC 5 cut(s) 114, 117, 120, 276, 386
FspBI CTAG 2 cut(s) 216, 357
GluI GCNGC 5 cut(s) 114, 117, 120, 276, 386
GsuI CTGGAG 1 cut(s) 253
HaeIII GGCC 2 cut(s) 315, 355
Hin1II CATG 1 cut(s) 343
HinfI GANTC 1 cut(s) 225
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 2 cut(s) 229, 270
Hpy8I GTNNAC 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 5 cut(s) 91, 113, 128, 309, 376
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 113, 119, 125, 382
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 1 cut(s) 343
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 5 cut(s) 69, 91, 96, 273, 283
Lsp1109I GCAGC 3 cut(s) 125, 128, 262
MaeI CTAG 2 cut(s) 216, 357
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 335
MboII GAAGA 2 cut(s) 304, 377
MhlI GDGCHC 1 cut(s) 269
MluCI AATT 2 cut(s) 65, 321
MlyI GAGTC 1 cut(s) 234
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 3 cut(s) 28, 189, 361
MroXI GAANNNNTTC 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 84
MwoI GCNNNNNNNGC 5 cut(s) 104, 113, 119, 125, 382
NlaIII CATG 1 cut(s) 343
NmuCI GTSAC 1 cut(s) 335
NspI RCATGY 1 cut(s) 343
PciI ACATGT 1 cut(s) 339
PdmI GAANNNNTTC 1 cut(s) 369
PkrI GCNGC 5 cut(s) 115, 118, 121, 277, 387
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
Ppu21I YACGTR 1 cut(s) 379
PscI ACATGT 1 cut(s) 339
Psp124BI GAGCTC 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 82
PspPI GGNCC 2 cut(s) 313, 353
RsaI GTAC 2 cut(s) 298, 381
RsaNI GTAC 2 cut(s) 297, 380
SacI GAGCTC 1 cut(s) 269
SatI GCNGC 5 cut(s) 114, 117, 120, 276, 386
Sau96I GGNCC 2 cut(s) 313, 353
ScaI AGTACT 1 cut(s) 298
SchI GAGTC 1 cut(s) 234
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 1 cut(s) 269
SetI ASST 8 cut(s) 39, 88, 200, 207, 269, 277, 372, 381
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 2 cut(s) 65, 321
SsiI CCGC 1 cut(s) 119
SspMI CTAG 2 cut(s) 216, 357
SstI GAGCTC 1 cut(s) 269
StyD4I CCNGG 1 cut(s) 82
StyI CCWWGG 1 cut(s) 389
TaiI ACGT 1 cut(s) 381
TasI AATT 2 cut(s) 65, 321
TatI WGTACW 1 cut(s) 296
TauI GCSGC 1 cut(s) 122
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 4 cut(s) 113, 116, 275, 385
Tsp45I GTSAC 1 cut(s) 335
TspGWI ACGGA 1 cut(s) 47
XapI RAATTY 1 cut(s) 65
XceI RCATGY 1 cut(s) 343
XmnI GAANNNNTTC 1 cut(s) 369
XspI CTAG 2 cut(s) 216, 357
ZrmI AGTACT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.