RLG00000005615

Mur ligase family, glutamate ligase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018433
Physical Location & Seq
Forward (+)
302559 .. 304300
1742 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005615

Sequence Viewer

Length: 687 bp
ATGTGTGTGGTTCTCAATGCTGATGATCCATTAGTTATGAGCCTCCTCCCTCTACCATTTGGGGTTCAAAAGGTGCTCTTTGGTCAGACGGGGGGGGGGGGTGTGATGTTCGGTTGGTTGAGTGCCAAAGCACACACGGAGGTCTTGGAGTATCATACAGAGATGGTAGAATTTGTGATACCCAGTCCAGGTCTTCATTTGGCTCTCAATGCTCGTGCAGCAGCGGCAGTTGCAACTCTTTTAGGAGTTTCTCTTTCTCAAGTTGGAATTTGTCTATCAGCATTTACCCCCGTCAAAATGAGGTCAGAGCTTGAAGTTGCTAGAAATGGAGTCAAGATAGTCAATGATGTTTACAATGCCAATCCTGTGAACACCAGAGCTGCCATTGACTTATTGAAAAGCATTGATTGCAAGGGCCAAAAAATTGCCATACTTGGTGACATGTTAGAACTCGGTGCAATTGAGTTGGAGTCTCACAAGATGATTCTTAAGCATTGTCATAGTGCTCATGATATTGATTTAATTGGCCTAGTTGGGAAGAGGTTCTGCACAGCAGCCAAGGATATGAATTTGCTTGAAGAGTTTAATATTGTGTGCTCTCTTGAGCCAGAAACTTTTGCAGAGGAAATTGTGAAGAGGATCAATCGTAATGATGTTATTTTGGTAAAGGGTAGTCGAGGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase_C Mur_ligase_M

Protein Analysis

229

Amino Acids

24.65

Weight (kDa)

5.97

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mur_ligase_C PF02875 101 - 226 2.1e-12 Mur ligase, glutamate ligase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018433)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37200
malus_domestica MD13G1002800.v1.1
prunus_persica Prupe.1G349600_v2.0.a1
rosa_laevigata RLG00000005615
rosa_rugosa Rorug04G0376600
rosa_samantha Rh4AG438200 Rh4BG447200 Rh4CG464400 Rh4DG445900
rosa_wichuraiana Rw4G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 224
AclWI GGATC 2 cut(s) 20, 647
AcsI RAATTY 3 cut(s) 170, 267, 568
AfiI CCNNNNNNNGG 1 cut(s) 188
AflII CTTAAG 1 cut(s) 488
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 4 cut(s) 68, 314, 397, 578
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 2 cut(s) 310, 380
AluI AGCT 2 cut(s) 310, 380
Alw21I GWGCWC 3 cut(s) 78, 508, 599
Alw26I GTCTC 1 cut(s) 477
AlwI GGATC 2 cut(s) 20, 647
AoxI GGCC 2 cut(s) 415, 526
ApeKI GCWGC 4 cut(s) 218, 221, 380, 554
ApoI RAATTY 3 cut(s) 170, 267, 568
Asp700I GAANNNNTTC 1 cut(s) 542
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 1 cut(s) 449
BauI CACGAG 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 3 cut(s) 78, 508, 599
BbvI GCAGC 4 cut(s) 230, 233, 367, 566
BccI CCATC 1 cut(s) 157
BciT130I CCWGG 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 477
BfaI CTAG 2 cut(s) 321, 530
BfrI CTTAAG 1 cut(s) 488
BisI GCNGC 5 cut(s) 219, 222, 225, 381, 555
BlsI GCNGC 5 cut(s) 220, 223, 226, 382, 556
Bme1390I CCNGG 1 cut(s) 189
BmgT120I GGNCC 1 cut(s) 415
BmrFI CCNGG 1 cut(s) 189
BmrI ACTGGG 1 cut(s) 177
BmuI ACTGGG 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 185
BpuEI CTTGAG 2 cut(s) 243, 623
BsaJI CCNNGG 1 cut(s) 558
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 183
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 558
BseGI GGATG 1 cut(s) 687
BseLI CCNNNNNNNGG 1 cut(s) 188
BseNI ACTGG 1 cut(s) 183
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 4 cut(s) 230, 233, 367, 566
BsgI GTGCAG 2 cut(s) 237, 532
BshFI GGCC 2 cut(s) 417, 528
BsiHKAI GWGCWC 3 cut(s) 78, 508, 599
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 477
BsnI GGCC 2 cut(s) 417, 528
Bsp1286I GDGCHC 3 cut(s) 78, 508, 599
Bsp143I GATC 2 cut(s) 25, 639
BspACI CCGC 1 cut(s) 224
BspANI GGCC 2 cut(s) 417, 528
BspHI TCATGA 1 cut(s) 508
BspPI GGATC 2 cut(s) 20, 647
BspTI CTTAAG 1 cut(s) 488
BsrI ACTGG 1 cut(s) 183
BssECI CCNNGG 1 cut(s) 558
BssMI GATC 2 cut(s) 25, 639
BssSI CACGAG 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 558
Bst2BI CACGAG 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 189
Bst6I CTCTTC 3 cut(s) 533, 573, 629
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 1 cut(s) 408
BstF5I GGATG 1 cut(s) 687
BstKTI GATC 2 cut(s) 28, 642
BstMAI GTCTC 1 cut(s) 477
BstMBI GATC 2 cut(s) 25, 639
BstMWI GCNNNNNNNGC 5 cut(s) 209, 218, 224, 230, 408
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 187
BstV1I GCAGC 4 cut(s) 230, 233, 367, 566
BstV2I GAAGAC 1 cut(s) 185
BsuRI GGCC 2 cut(s) 417, 528
BtsCI GGATG 1 cut(s) 687
CciI TCATGA 1 cut(s) 508
Cfr13I GGNCC 1 cut(s) 415
CviAII CATG 2 cut(s) 442, 509
CviJI RGCY 8 cut(s) 42, 203, 310, 380, 417, 528, 557, 607
CviKI_1 RGCY 8 cut(s) 42, 203, 310, 380, 417, 528, 557, 607
DpnI GATC 2 cut(s) 27, 641
DpnII GATC 2 cut(s) 25, 639
Eam1104I CTCTTC 3 cut(s) 533, 573, 629
EarI CTCTTC 3 cut(s) 533, 573, 629
Eco130I CCWWGG 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 558
FaeI CATG 2 cut(s) 445, 512
FaiI YATR 7 cut(s) 38, 156, 431, 443, 501, 510, 566
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FatI CATG 2 cut(s) 441, 508
Fnu4HI GCNGC 5 cut(s) 219, 222, 225, 381, 555
Fsp4HI GCNGC 5 cut(s) 219, 222, 225, 381, 555
FspBI CTAG 2 cut(s) 321, 530
GluI GCNGC 5 cut(s) 219, 222, 225, 381, 555
HaeIII GGCC 2 cut(s) 417, 528
Hin1II CATG 2 cut(s) 445, 512
HinfI GANTC 3 cut(s) 330, 470, 484
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 2 cut(s) 352, 370
Hpy188I TCNGA 2 cut(s) 87, 307
Hpy188III TCNNGA 3 cut(s) 334, 509, 602
Hpy8I GTNNAC 2 cut(s) 352, 370
HpyCH4V TGCA 6 cut(s) 218, 233, 411, 458, 549, 620
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 218, 224, 230, 408
Hsp92II CATG 2 cut(s) 445, 512
Kzo9I GATC 2 cut(s) 25, 639
LpnPI CCDG 6 cut(s) 174, 196, 201, 378, 388, 621
Lsp1109I GCAGC 4 cut(s) 230, 233, 367, 566
MaeI CTAG 2 cut(s) 321, 530
MaeIII GTNAC 1 cut(s) 437
MalI GATC 2 cut(s) 27, 641
MboI GATC 2 cut(s) 25, 639
MboII GAAGA 4 cut(s) 185, 550, 590, 646
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 3 cut(s) 78, 508, 599
MluCI AATT 7 cut(s) 170, 267, 423, 459, 522, 568, 627
MlyI GAGTC 2 cut(s) 339, 479
MmeI TCCRAC 2 cut(s) 244, 447
MnlI CCTC 9 cut(s) 53, 56, 60, 133, 294, 534, 616, 630, 671
MroXI GAANNNNTTC 1 cut(s) 542
MseI TTAA 3 cut(s) 489, 521, 585
MspA1I CMGCKG 1 cut(s) 224
MspCI CTTAAG 1 cut(s) 488
MspR9I CCNGG 1 cut(s) 189
MunI CAATTG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 5 cut(s) 209, 218, 224, 230, 408
NdeII GATC 2 cut(s) 25, 639
NlaIII CATG 2 cut(s) 445, 512
NmuCI GTSAC 1 cut(s) 437
NspI RCATGY 1 cut(s) 445
PagI TCATGA 1 cut(s) 508
PciI ACATGT 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 1 cut(s) 484
PkrI GCNGC 5 cut(s) 220, 223, 226, 382, 556
PleI GAGTC 2 cut(s) 338, 478
PpsI GAGTC 2 cut(s) 338, 478
PscI ACATGT 1 cut(s) 441
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspPI GGNCC 1 cut(s) 415
SaqAI TTAA 3 cut(s) 489, 521, 585
SatI GCNGC 5 cut(s) 219, 222, 225, 381, 555
Sau3AI GATC 2 cut(s) 25, 639
Sau96I GGNCC 1 cut(s) 415
SchI GAGTC 2 cut(s) 339, 479
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 3 cut(s) 78, 508, 599
SetI ASST 7 cut(s) 75, 144, 193, 305, 312, 382, 545
SmlI CTYRAG 3 cut(s) 258, 488, 602
SmoI CTYRAG 3 cut(s) 258, 488, 602
Sse9I AATT 7 cut(s) 170, 267, 423, 459, 522, 568, 627
SsiI CCGC 1 cut(s) 224
SspI AATATT 1 cut(s) 589
SspMI CTAG 2 cut(s) 321, 530
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 1 cut(s) 558
TaqI TCGA 1 cut(s) 676
TasI AATT 7 cut(s) 170, 267, 423, 459, 522, 568, 627
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 484
Tru1I TTAA 3 cut(s) 489, 521, 585
Tru9I TTAA 3 cut(s) 489, 521, 585
TseFI GTSAC 1 cut(s) 437
TseI GCWGC 4 cut(s) 218, 221, 380, 554
Tsp45I GTSAC 1 cut(s) 437
TspDTI ATGAA 2 cut(s) 185, 581
TspGWI ACGGA 1 cut(s) 152
Vha464I CTTAAG 1 cut(s) 488
XapI RAATTY 3 cut(s) 170, 267, 568
XceI RCATGY 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 2 cut(s) 321, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.