Rh4CG464400

Mur ligase family, glutamate ligase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018433
Physical Location & Seq
Reverse (-)
69454887 .. 69462269
7383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG464400.1

Sequence Viewer

Length: 819 bp
ATGGCCAAAGGGGAGATTCTCCAGGAGGCAAAGCTTGGAGATGTGTGTGTTCTCCATGCTGCTGATCCATTAGTTATGAGCCTCCTCCCTCTACCATTTGGGGTTCAAAAGGTGCTCTTTGGTCAGACGGCGGGGTGTGATGTTCGTTTGGTTGAGTGCGAAAGCACACACGGAGGTCTTGGAGTATCATACAGAGATTTGACAATGAACATTCTAGCTAATTCCCTCACTATTGTTGAACAAATCCAAAAGCTTTCGTCAATCACCAGAGCTGCCTGCCAGGTAGAATTTGTGATACCCAGTCGAGGTCTGCATTTGGCTCTCAATGCTTGTGCAGCAGCGGCAGTTGCAACTCTTTTAGGAGTTTCTCTTTCTCAAGTTGGAATTTGTCTATCAGCATTTACTCCCGTCAAAATGAGGTCAGAGCTTGAAGTTGCTAGAAATGGAGTCAAGATAGTCAATGATGTTTACAATGCCAATCCTGCGAGCACCAGAGCTGCCATTGACATATTGAAGAGTATTGATTGCAAGGGCCAAAAAATTGCCATACTTGGTGACATGTTAGAACTCGGTGCCATTGAGTTGGAGTCTCACAAGATGATTCTTAAGCATTGTCATAGTGCTCATGATATTGTTTTAATTGGCCTAGTTGGGAAGAGGTTCTGCACAGCAGCCAAGGATATGAATTTGCTTGAAGAGTTTAATATTGTGTGCTCTCTTGAGCCAGAAACTTTTGCAGAGGAAAATGTGAAGAGGATCAATTGTAATGATGTTATTTTGGTAAAGGGTAGTCGAGATAGAGTTAATATACCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mur_ligase_C Mur_ligase_M

Protein Analysis

272

Amino Acids

29.24

Weight (kDa)

5.99

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mur_ligase_C PF02875 140 - 265 6.3e-13 Mur ligase, glutamate ligase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018433)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g37200
malus_domestica MD13G1002800.v1.1
prunus_persica Prupe.1G349600_v2.0.a1
rosa_laevigata RLG00000005615
rosa_rugosa Rorug04G0376600
rosa_samantha Rh4AG438200 Rh4BG447200 Rh4CG464400 Rh4DG445900
rosa_wichuraiana Rw4G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 572
AciI CCGC 2 cut(s) 131, 341
AclWI GGATC 2 cut(s) 59, 764
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 287, 384, 685
AfiI CCNNNNNNNGG 1 cut(s) 305
AflII CTTAAG 1 cut(s) 605
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 5 cut(s) 107, 239, 431, 514, 695
AjnI CCWGG 2 cut(s) 21, 279
AluBI AGCT 6 cut(s) 34, 218, 253, 272, 427, 497
AluI AGCT 6 cut(s) 34, 218, 253, 272, 427, 497
Alw21I GWGCWC 4 cut(s) 117, 491, 625, 716
Alw26I GTCTC 1 cut(s) 594
AlwI GGATC 2 cut(s) 59, 764
AoxI GGCC 3 cut(s) 3, 532, 643
ApeKI GCWGC 6 cut(s) 59, 272, 335, 338, 497, 671
ApoI RAATTY 3 cut(s) 287, 384, 685
Asp700I GAANNNNTTC 1 cut(s) 659
AspS9I GGNCC 1 cut(s) 532
AsuHPI GGTGA 2 cut(s) 256, 566
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 572
Bbv12I GWGCWC 4 cut(s) 117, 491, 625, 716
BbvI GCAGC 6 cut(s) 46, 259, 347, 350, 484, 683
BceAI ACGGC 1 cut(s) 144
BciT130I CCWGG 2 cut(s) 23, 281
BcoDI GTCTC 1 cut(s) 594
BfaI CTAG 4 cut(s) 215, 438, 647, 817
BfrI CTTAAG 1 cut(s) 605
BisI GCNGC 7 cut(s) 60, 273, 336, 339, 342, 498, 672
BlsI GCNGC 7 cut(s) 61, 274, 337, 340, 343, 499, 673
Bme1390I CCNGG 2 cut(s) 23, 281
BmgT120I GGNCC 1 cut(s) 532
BmiI GGNNCC 1 cut(s) 574
BmrFI CCNGG 2 cut(s) 23, 281
BmrI ACTGGG 1 cut(s) 294
BmuI ACTGGG 1 cut(s) 294
BpmI CTGGAG 1 cut(s) 5
BpuEI CTTGAG 2 cut(s) 360, 740
BsaJI CCNNGG 1 cut(s) 675
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse1I ACTGG 1 cut(s) 300
BseBI CCWGG 2 cut(s) 23, 281
BseDI CCNNGG 1 cut(s) 675
BseLI CCNNNNNNNGG 1 cut(s) 305
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 74
BseXI GCAGC 6 cut(s) 46, 259, 347, 350, 484, 683
BsgI GTGCAG 2 cut(s) 354, 649
BshFI GGCC 3 cut(s) 5, 534, 645
BshNI GGYRCC 1 cut(s) 572
BsiHKAI GWGCWC 4 cut(s) 117, 491, 625, 716
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 594
BsnI GGCC 3 cut(s) 5, 534, 645
Bsp1286I GDGCHC 4 cut(s) 117, 491, 625, 716
Bsp143I GATC 2 cut(s) 64, 756
BspACI CCGC 2 cut(s) 131, 341
BspANI GGCC 3 cut(s) 5, 534, 645
BspHI TCATGA 1 cut(s) 625
BspLI GGNNCC 1 cut(s) 574
BspPI GGATC 2 cut(s) 59, 764
BspT107I GGYRCC 1 cut(s) 572
BspTI CTTAAG 1 cut(s) 605
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 675
BssMI GATC 2 cut(s) 64, 756
BssT1I CCWWGG 1 cut(s) 675
Bst2UI CCWGG 2 cut(s) 23, 281
Bst6I CTCTTC 4 cut(s) 509, 650, 690, 746
BstAFI CTTAAG 1 cut(s) 605
BstC8I GCNNGC 2 cut(s) 277, 487
BstKTI GATC 2 cut(s) 67, 759
BstMAI GTCTC 1 cut(s) 594
BstMBI GATC 2 cut(s) 64, 756
BstMWI GCNNNNNNNGC 5 cut(s) 326, 335, 341, 347, 482
BstNI CCWGG 2 cut(s) 23, 281
BstNSI RCATGY 1 cut(s) 562
BstSCI CCNGG 2 cut(s) 21, 279
BstV1I GCAGC 6 cut(s) 46, 259, 347, 350, 484, 683
BstXI CCANNNNNNTGG 1 cut(s) 583
BsuRI GGCC 3 cut(s) 5, 534, 645
Cac8I GCNNGC 2 cut(s) 277, 487
CciI TCATGA 1 cut(s) 625
Cfr13I GGNCC 1 cut(s) 532
CviAII CATG 3 cut(s) 56, 559, 626
DpnI GATC 2 cut(s) 66, 758
DpnII GATC 2 cut(s) 64, 756
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 509, 650, 690, 746
EarI CTCTTC 4 cut(s) 509, 650, 690, 746
Eco130I CCWWGG 1 cut(s) 675
EcoRII CCWGG 2 cut(s) 21, 279
EcoT14I CCWWGG 1 cut(s) 675
ErhI CCWWGG 1 cut(s) 675
FaeI CATG 3 cut(s) 59, 562, 629
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FatI CATG 3 cut(s) 55, 558, 625
FauI CCCGC 1 cut(s) 124
Fnu4HI GCNGC 7 cut(s) 60, 273, 336, 339, 342, 498, 672
Fsp4HI GCNGC 7 cut(s) 60, 273, 336, 339, 342, 498, 672
FspBI CTAG 4 cut(s) 215, 438, 647, 817
GluI GCNGC 7 cut(s) 60, 273, 336, 339, 342, 498, 672
GsuI CTGGAG 1 cut(s) 5
HaeIII GGCC 3 cut(s) 5, 534, 645
Hin1II CATG 3 cut(s) 59, 562, 629
HindIII AAGCTT 2 cut(s) 32, 251
HinfI GANTC 4 cut(s) 16, 447, 587, 601
HphI GGTGA 2 cut(s) 256, 566
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 2 cut(s) 126, 424
Hpy188III TCNNGA 4 cut(s) 451, 626, 719, 794
Hpy8I GTNNAC 1 cut(s) 469
HpyCH4V TGCA 6 cut(s) 313, 335, 350, 528, 666, 737
HpyF10VI GCNNNNNNNGC 5 cut(s) 326, 335, 341, 347, 482
Hsp92II CATG 3 cut(s) 59, 562, 629
Kzo9I GATC 2 cut(s) 64, 756
Lsp1109I GCAGC 6 cut(s) 46, 259, 347, 350, 484, 683
MaeI CTAG 4 cut(s) 215, 438, 647, 817
MaeIII GTNAC 1 cut(s) 554
MalI GATC 2 cut(s) 66, 758
MboI GATC 2 cut(s) 64, 756
MboII GAAGA 4 cut(s) 526, 667, 707, 763
MfeI CAATTG 1 cut(s) 760
MhlI GDGCHC 4 cut(s) 117, 491, 625, 716
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 220, 287, 384, 540, 639, 685, 760
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 456, 596
MmeI TCCRAC 2 cut(s) 361, 564
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 659
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 606, 638, 702, 804
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 341
MspCI CTTAAG 1 cut(s) 605
MspR9I CCNGG 2 cut(s) 23, 281
MunI CAATTG 1 cut(s) 760
MvaI CCWGG 2 cut(s) 23, 281
MwoI GCNNNNNNNGC 5 cut(s) 326, 335, 341, 347, 482
NdeII GATC 2 cut(s) 64, 756
NlaIII CATG 3 cut(s) 59, 562, 629
NlaIV GGNNCC 1 cut(s) 574
NmuCI GTSAC 1 cut(s) 554
NspI RCATGY 1 cut(s) 562
PagI TCATGA 1 cut(s) 625
PciI ACATGT 1 cut(s) 558
PdmI GAANNNNTTC 1 cut(s) 659
PfeI GAWTC 2 cut(s) 16, 601
PfoI TCCNGGA 1 cut(s) 21
PkrI GCNGC 7 cut(s) 61, 274, 337, 340, 343, 499, 673
PleI GAGTC 2 cut(s) 455, 595
PpsI GAGTC 2 cut(s) 455, 595
PscI ACATGT 1 cut(s) 558
Psp6I CCWGG 2 cut(s) 21, 279
PspGI CCWGG 2 cut(s) 21, 279
PspN4I GGNNCC 1 cut(s) 574
PspPI GGNCC 1 cut(s) 532
SaqAI TTAA 4 cut(s) 606, 638, 702, 804
SatI GCNGC 7 cut(s) 60, 273, 336, 339, 342, 498, 672
Sau3AI GATC 2 cut(s) 64, 756
Sau96I GGNCC 1 cut(s) 532
SchI GAGTC 2 cut(s) 456, 596
ScrFI CCNGG 2 cut(s) 23, 281
SduI GDGCHC 4 cut(s) 117, 491, 625, 716
SmlI CTYRAG 3 cut(s) 375, 605, 719
SmoI CTYRAG 3 cut(s) 375, 605, 719
Sse9I AATT 7 cut(s) 220, 287, 384, 540, 639, 685, 760
SsiI CCGC 2 cut(s) 131, 341
SspI AATATT 1 cut(s) 706
SspMI CTAG 4 cut(s) 215, 438, 647, 817
StyD4I CCNGG 2 cut(s) 21, 279
StyI CCWWGG 1 cut(s) 675
TaqI TCGA 2 cut(s) 304, 793
TasI AATT 7 cut(s) 220, 287, 384, 540, 639, 685, 760
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 16, 601
Tru1I TTAA 4 cut(s) 606, 638, 702, 804
Tru9I TTAA 4 cut(s) 606, 638, 702, 804
TseFI GTSAC 1 cut(s) 554
TseI GCWGC 6 cut(s) 59, 272, 335, 338, 497, 671
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 2 cut(s) 221, 698
TspGWI ACGGA 1 cut(s) 186
Vha464I CTTAAG 1 cut(s) 605
XapI RAATTY 3 cut(s) 287, 384, 685
XceI RCATGY 1 cut(s) 562
XmnI GAANNNNTTC 1 cut(s) 659
XspI CTAG 4 cut(s) 215, 438, 647, 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.