Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0007854

Physical Location & Seq

Genomic Coordinates Reverse (-)

1926027 .. 1933062

Gene Structure 7036 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g03250.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2655 bp

ATGACCGGGACGACGCTCACACCGCAGTCGGCGAGTAACTTAGTCAGTCTCAACATTGGCGGCGGCGCCGGGCCACAGCTCTCGGCTTCGTTCGTGAACTCCTTCCGAGTCACCGCCGTCGCCGAGCGGCTCCTCAACCACGTTCAGTCTGGTTTTCGCGGTGACGTCATGGAGTTCTTCAACCTCTGCCTTTCTCTCGCCAGAGGGATTGATTATGCTGTTGCGAACAATGAGGTTCCGGCGAAGGCGAGCGATTTGCCTAGTCTGTTGAGACAGATATGCCAAAGGAAAAACAACGACGTTTTGGTAGCTGCTGTTATGGTGCTGATGATATCTGTGAAGAATGCTTGTAGGACTGGATGGTTTTCGGAGAAGGAGAGTGAAGAGCTGTTTTCTCTAGCGAATGAGATTGGGAGTAGGTTCTGTGGCTCGGGGGATATCACAACTGGATCTGACTGTTCTCTATCTATTGTTGACAAAGTTATAGAAAGATATTATCCAACGTTGAAGATGGGTCAGACTCTTGCCTCTCTCGAAGTTAAGCCTGGATATGGAACATACGTGCTTGATTTCCATATTTCAAAGAGTACAGGTTATGCTTCCCATGAAAAAATTCGGCTATTTGTAGCCCAAACTGATAATATCGAGACATCTTCTTGTATTATAAGTCCCCAACAAGTGAACTTTCTTCTGAATGGAAAGGGAGTAGATAAGAGGATCACTTCTACAATGGATACTGGACCACAGTTGCCTACAAATGTGACTGGAATGCTTAAATATGGAACAAATCTTCTTCAAGCTGTGGGCCAGTTCAATGGTCACTATATCATAGTGATTGCCTTCATGAGCATCGCATCATCAATTGACCCTCCAGTACTGAAAGATTATGTTCAGCCCATTGAACCTTCCTCAAAGTCAGATACTGATATAGTTGAAGGGCCATCAAGAATATCACTTAATTGTCCCATAAGCTTTACGCGAATCCAAACTCCTGTTAAAGGACATTTGTGCAAACATCTTCAGTGCTTCGACTTCAGGAACTACATCACTATGAACATAAGACGACCAAGGTGGCGCTGCCCCCATTGTGATCAGTATGTTTGCTATTTGGACATTCGTGTCGATCAAAATATAGTTAAGGTCCTGAGAGAGGTAGGAGAGAATGTTTCTAAAGTAAATATCTCTGTAGATGGATCATGGAAGGTGTCGGAAAATGATGATGATGATCTATATCTGGTAGACAAACTAAACGAAACATCTGAGATGGATGAAGCTACTCCTGGTCCTAGTGTTATGGATCTTACCAATGATGATGATACTGAGATGGCTAGTGCTTGCGAACCTGAAGATGTGAAACCTTTGAGCAATACAAACACTGCTCAAGTAGAAAATGACTTTTGGTCTGGAGTTTACTTGGCTAATTGTACGTCTTACTCTGGTCCCAGTTCTTTACAACCTTCCTTGTTGTCTGATGCTGTTTCACTTGCTCTTAATCGAGAAGCCGGGAGTCATGGGAACACTGATTTTTTGGTGTCTGCAATGCATAATCAACTCTCTACTCCCAATTACGTGAACTCACAGTTGTTGCAATCTGCAAATTCGACTGCTAGCAATGAGTATGGGACATCACAGACATTACCTAGAGAACTGGCTTCCGGGCTGCCAATAGGCAGGACTCCGACAGCAGTTCAGGCCCTACCAGCTTTGTCCCAGACACTTGGTGTGCAACAAAGACCCAGAACCAGTTTCAATAATCCAGCTCCCAATAGTGCCTCCCTCACTTCTCATGCTGGCCAGTCTATCACTTCCGAGGCAAATGTTCTCAGTGGAATCTGTAGCGACTTGGAAAGGCAACAATACTTTGCTAGGCCCCAAATGAATCCAGTTCAAGTGTCAGGCATTGCTTCATCCTCATTGCAGCATGGCTCAAGAACAACTCAGAATTGTGCCCCCCAAGATCGGTCCTTTACTCATGGCCAATCCGTTGTTGGACATCAGGTTCCGAGCCAACTGCAAAGCGCTAGCAGAGTATCTTCTGGGCTCCAGGGGTTCACAAATGCACACCTAGATAAAACATTTAATTCGAGGACTCCACCAGCTATGAGCCAATCTCCAAGGGTACATACTTTACGGTCCCATGTAAGGCAGGGGAGTGCACAAGTTGGAATTAATCAAGCACCAAGTTCTTTAAACAACCAACAGAGCTTCACAGTGGCTGCTCAGCGAGCTGCTATGGCCAGGCAGTCTTCACCAATGCCAGCTCAAAATCAAACTCCCAGAACCAGGCCATCTTTGTCTGTAAATGCTGGAGGGTTTAGAGGGTCGGCAGGGGATCGACGTGGAAACATTGGAGGTCCTGTCCAAGCAGTTTCAGGGGCTGATGAGTTGGTGGATTCACCATCTGAACAGAATTGGCGACCAACAGGTCGAATGCGTGGAAGTCTCTCTGGTCAGGCTTATACTGCTGCTTTGAACAAGTTCATAATTCGGCCAACCCCACCAACTGAAAAAGCGAGACCTGCGTCACACCTGACCCCTCCTTCACACCTGACCCGTCCTCCACACCTGACCTCTCCACCAGCTGTTGTTCCATCCCAATTGCCAACTGATATGAGCTCTCAGCTTCCTCAAATGCCTACTAGTCAGCCTCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0006464 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009893 GO:0009987 GO:0010033 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0031323 GO:0031325 GO:0032446 GO:0033993 GO:0036211 GO:0042221 GO:0042762 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051176 GO:0065007 GO:0070647 GO:0071704 GO:0097305 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04120 ko04630 ko05160 map04120 map04630 map05160

Pfam Domains

Protein Families

zf-MIZ

Protein Analysis

885

Amino Acids

95.47

Weight (kDa)

6.83

Isoelectric Point (pI)

49.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-Nse	PF11789	123 - 171	3.4e-06	Zinc-finger of the MIZ type in Nse subunit
zf-MIZ	PF02891	125 - 173	3.9e-20	MIZ/SP-RING zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007854)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41580
fragaria_vesca	FvH4_5g03250 FvH4_5g03250 FvH4_5g03250 FvH4_5g03250 FvH4_5g03250
malus_domestica	MD06G1091800.v1.1 MD14G1112600.v1.1
prunus_persica	Prupe.5G107000_v2.0.a1 Prupe.5G107000_v2.0.a1
pyrus_communis	pycom06g08790 pycom14g10780
rosa_chinensis	RchiOBHm_Chr7g0196521
rosa_multiflora	Rmu_co8354373.1_g000001 Rmu_co8377057.1_g000001 Rmu_sc0013350.1_g000009 Rmu_sc0019310.1_g000002
rosa_roxburghii	Rroxscaffold_3G00259230
rosa_rugosa	Rorug07G0034400
rosa_samantha	Rh7AG158500 Rh7BG160500 Rh7CG166900 Rh7DG159700
rosa_wichuraiana	Rw7G013850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	665
AasI	GACNNNNNNGTC	2 cut(s)	1118, 2424
AatII	GACGTC	1 cut(s)	168
Acc36I	ACCTGC	1 cut(s)	2527
AccB1I	GGYRCC	1 cut(s)	65
AccBSI	CCGCTC	1 cut(s)	127
AccI	GTMKAC	1 cut(s)	1239
AccII	CGCG	2 cut(s)	159, 979
AciI	CCGC	6 cut(s)	23, 60, 63, 114, 127, 159
AclI	AACGTT	1 cut(s)	503
AclWI	GGATC	5 cut(s)	457, 725, 1201, 1305, 2340
AcoI	YGGCCR	4 cut(s)	1792, 1975, 2235, 2489
AcsI	RAATTY	2 cut(s)	612, 1597
AcuI	CTGAAG	3 cut(s)	1004, 1018, 1365
AcyI	GRCGYC	2 cut(s)	66, 165
AdeI	CACNNNGTG	1 cut(s)	1721
AfaI	GTAC	4 cut(s)	589, 876, 1426, 2121
AfeI	AGCGCT	1 cut(s)	2020
AfiI	CCNNNNNNNGG	6 cut(s)	551, 998, 1150, 1959, 2142, 2283
AhdI	GACNNNNNGTC	1 cut(s)	1399
AhlI	ACTAGT	1 cut(s)	2639
AjiI	CACGTC	1 cut(s)	2339
AjnI	CCWGG	5 cut(s)	544, 1279, 2043, 2237, 2282
AloI	GAACNNNNNNTCC	2 cut(s)	1983, 2015
Alw21I	GWGCWC	2 cut(s)	2158, 2618
Alw26I	GTCTC	5 cut(s)	53, 265, 641, 2447, 2509
Alw44I	GTGCAC	1 cut(s)	2154
AlwI	GGATC	5 cut(s)	457, 725, 1201, 1305, 2340
AlwNI	CAGNNNCTG	3 cut(s)	923, 2216, 2378
Ama87I	CYCGRG	1 cut(s)	430
Aor51HI	AGCGCT	1 cut(s)	2020
ApaLI	GTGCAC	1 cut(s)	2154
ApeKI	GCWGC	7 cut(s)	311, 1077, 1660, 1918, 2216, 2228, 2465
ApoI	RAATTY	2 cut(s)	612, 1597
AseI	ATTAAT	1 cut(s)	2169
Asp700I	GAANNNNTTC	3 cut(s)	101, 612, 2477
AspLEI	GCGC	3 cut(s)	68, 1077, 2021
AsuC2I	CCSGG	4 cut(s)	7, 70, 1504, 1657
AsuHPI	GGTGA	4 cut(s)	103, 173, 2241, 2388
AsuNHI	GCTAGC	2 cut(s)	1607, 2021
AvaI	CYCGRG	1 cut(s)	430
AvaII	GGWCC	7 cut(s)	740, 1141, 1283, 1439, 1962, 2133, 2354
BaeGI	GKGCMC	2 cut(s)	1951, 2158
BalI	TGGCCA	3 cut(s)	1794, 1977, 2237
BanI	GGYRCC	1 cut(s)	65
BanII	GRGCYC	2 cut(s)	2043, 2618
BbsI	GAAGAC	1 cut(s)	2238
Bbv12I	GWGCWC	2 cut(s)	2158, 2618
BbvI	GCAGC	7 cut(s)	298, 1064, 1647, 1930, 2203, 2215, 2452
BccI	CCATC	9 cut(s)	354, 505, 949, 1184, 1258, 1318, 2296, 2407, 2599
BceAI	ACGGC	1 cut(s)	101
BcgI	CGANNNNNNTGC	4 cut(s)	238, 272, 1993, 2027
BciT130I	CCWGG	5 cut(s)	546, 1281, 2045, 2239, 2284
BciVI	GTATCC	1 cut(s)	727
BclI	TGATCA	1 cut(s)	1090
BcnI	CCSGG	4 cut(s)	7, 70, 1504, 1657
BcoDI	GTCTC	5 cut(s)	53, 265, 641, 2447, 2509
BcuI	ACTAGT	1 cut(s)	2639
BfmI	CTRYAG	2 cut(s)	1185, 1834
BfoI	RGCGCY	3 cut(s)	69, 1078, 2022
BfuAI	ACCTGC	1 cut(s)	2527
BfuI	GTATCC	1 cut(s)	727
BlpI	GCTNAGC	1 cut(s)	2220
BmcAI	AGTACT	1 cut(s)	876
Bme1390I	CCNGG	9 cut(s)	7, 70, 546, 1281, 1504, 1657, 2045, 2239, 2284
Bme18I	GGWCC	7 cut(s)	740, 1141, 1283, 1439, 1962, 2133, 2354
BmeRI	GACNNNNNGTC	1 cut(s)	1399
BmeT110I	CYCGRG	1 cut(s)	430
BmgBI	CACGTC	1 cut(s)	2339
BmiI	GGNNCC	8 cut(s)	67, 131, 237, 1441, 1871, 2001, 2042, 2135
BmrFI	CCNGG	9 cut(s)	7, 70, 546, 1281, 1504, 1657, 2045, 2239, 2284
BmrI	ACTGGG	1 cut(s)	1437
BmsI	GCATC	3 cut(s)	858, 863, 1462
BmtI	GCTAGC	2 cut(s)	1611, 2025
BmuI	ACTGGG	1 cut(s)	1437
BoxI	GACNNNNGTC	1 cut(s)	2521
BpiI	GAAGAC	1 cut(s)	2238
BplI	GAGNNNNNCTC	2 cut(s)	2095, 2127
BpmI	CTGGAG	4 cut(s)	855, 1425, 2027, 2328
Bpu1102I	GCTNAGC	1 cut(s)	2220
BpuEI	CTTGAG	2 cut(s)	1365, 1912
BpuMI	CCSGG	4 cut(s)	7, 70, 1504, 1657
BsaAI	YACGTR	2 cut(s)	562, 1570
BsaBI	GATNNNNATC	2 cut(s)	1224, 1230
BsaHI	GRCGYC	2 cut(s)	66, 165
BsaI	GGTCTC	1 cut(s)	2509
BsaJI	CCNNGG	4 cut(s)	1067, 1809, 2044, 2114
BsaXI	ACNNNNNCTCC	6 cut(s)	1149, 1179, 2343, 2373, 2542, 2572
Bsc4I	CCNNNNNNNGG	6 cut(s)	551, 998, 1150, 1959, 2142, 2283
Bse3DI	GCAATG	4 cut(s)	1545, 1618, 1899, 1913
Bse8I	GATNNNNATC	2 cut(s)	1224, 1230
BseBI	CCWGG	5 cut(s)	546, 1281, 2045, 2239, 2284
BseDI	CCNNGG	4 cut(s)	1067, 1809, 2044, 2114
BseGI	GGATG	4 cut(s)	365, 1273, 1907, 2591
BseJI	GATNNNNATC	2 cut(s)	1224, 1230
BseLI	CCNNNNNNNGG	6 cut(s)	551, 998, 1150, 1959, 2142, 2283
BseMI	GCAATG	4 cut(s)	1545, 1618, 1899, 1913
BseMII	CTCAG	7 cut(s)	1136, 1251, 1311, 1837, 1952, 2234, 2633
BseRI	GAGGAG	1 cut(s)	122
BseSI	GKGCMC	2 cut(s)	1951, 2158
BseXI	GCAGC	7 cut(s)	298, 1064, 1647, 1930, 2203, 2215, 2452
Bsh1236I	CGCG	2 cut(s)	159, 979
BshNI	GGYRCC	1 cut(s)	65
BsiHKAI	GWGCWC	2 cut(s)	2158, 2618
BsiHKCI	CYCGRG	1 cut(s)	430
BsiSI	CCGG	5 cut(s)	6, 69, 239, 1503, 1656
BslFI	GGGAC	7 cut(s)	22, 654, 948, 1425, 1636, 1693, 2119
BslI	CCNNNNNNNGG	6 cut(s)	551, 998, 1150, 1959, 2142, 2283
BsmAI	GTCTC	5 cut(s)	53, 265, 641, 2447, 2509
BsmFI	GGGAC	7 cut(s)	22, 654, 948, 1425, 1636, 1693, 2119
BsmI	GAATGC	3 cut(s)	349, 774, 2436
Bso31I	GGTCTC	1 cut(s)	2509
BsoBI	CYCGRG	1 cut(s)	430
Bsp1286I	GDGCHC	4 cut(s)	1951, 2043, 2158, 2618
Bsp143I	GATC	9 cut(s)	449, 717, 1090, 1123, 1193, 1225, 1297, 1957, 2332
Bsp1720I	GCTNAGC	1 cut(s)	2220
BspACI	CCGC	6 cut(s)	23, 60, 63, 114, 127, 159
BspCNI	CTCAG	7 cut(s)	1137, 1252, 1312, 1836, 1951, 2233, 2632
BspFNI	CGCG	2 cut(s)	159, 979
BspHI	TCATGA	1 cut(s)	843
BspLI	GGNNCC	8 cut(s)	67, 131, 237, 1441, 1871, 2001, 2042, 2135
BspMI	ACCTGC	1 cut(s)	2527
BspOI	GCTAGC	2 cut(s)	1611, 2025
BspPI	GGATC	5 cut(s)	457, 725, 1201, 1305, 2340
BspQI	GCTCTTC	1 cut(s)	378
BspT107I	GGYRCC	1 cut(s)	65
BspTNI	GGTCTC	1 cut(s)	2509
BsrBI	CCGCTC	1 cut(s)	127
BsrDI	GCAATG	4 cut(s)	1545, 1618, 1899, 1913
BssECI	CCNNGG	4 cut(s)	1067, 1809, 2044, 2114
BssMI	GATC	9 cut(s)	449, 717, 1090, 1123, 1193, 1225, 1297, 1957, 2332
BssNI	GRCGYC	2 cut(s)	66, 165
BssT1I	CCWWGG	2 cut(s)	1067, 2114
Bst2UI	CCWGG	5 cut(s)	546, 1281, 2045, 2239, 2284
Bst4CI	ACNGT	5 cut(s)	458, 747, 1581, 2133, 2212
Bst6I	CTCTTC	1 cut(s)	378
BstACI	GRCGYC	2 cut(s)	66, 165
BstBAI	YACGTR	2 cut(s)	562, 1570
BstC8I	GCNNGC	7 cut(s)	250, 1336, 1609, 1792, 2023, 2226, 2259
BstDEI	CTNAG	8 cut(s)	40, 1145, 1260, 1320, 1823, 1938, 2220, 2619
BstENI	CCTNNNNNAGG	2 cut(s)	996, 1148
BstF5I	GGATG	4 cut(s)	365, 1273, 1907, 2591
BstFNI	CGCG	2 cut(s)	159, 979
BstH2I	RGCGCY	3 cut(s)	69, 1078, 2022
BstHHI	GCGC	3 cut(s)	68, 1077, 2021
BstKTI	GATC	9 cut(s)	452, 720, 1093, 1126, 1196, 1228, 1300, 1960, 2335
BstMAI	GTCTC	5 cut(s)	53, 265, 641, 2447, 2509
BstMBI	GATC	9 cut(s)	449, 717, 1090, 1123, 1193, 1225, 1297, 1957, 2332
BstMWI	GCNNNNNNNGC	7 cut(s)	22, 1691, 1700, 2225, 2234, 2462, 2519
BstNI	CCWGG	5 cut(s)	546, 1281, 2045, 2239, 2284
BstPAI	GACNNNNGTC	1 cut(s)	2521
BstSCI	CCNGG	9 cut(s)	5, 68, 544, 1279, 1502, 1655, 2043, 2237, 2282
BstSFI	CTRYAG	2 cut(s)	1185, 1834
BstSLI	GKGCMC	2 cut(s)	1951, 2158
BstUI	CGCG	2 cut(s)	159, 979
BstV1I	GCAGC	7 cut(s)	298, 1064, 1647, 1930, 2203, 2215, 2452
BstV2I	GAAGAC	1 cut(s)	2238
BstX2I	RGATCY	2 cut(s)	449, 1297
BstXI	CCANNNNNNTGG	2 cut(s)	815, 1718
BstYI	RGATCY	2 cut(s)	449, 1297
BsuI	GTATCC	1 cut(s)	727
BtgZI	GCGATG	1 cut(s)	835
BtrI	CACGTC	1 cut(s)	2339
BtsCI	GGATG	4 cut(s)	365, 1273, 1907, 2591
BtsI	GCAGTG	1 cut(s)	1374
BtsIMutI	CAGTG	5 cut(s)	1028, 1374, 1518, 1831, 2217
BveI	ACCTGC	1 cut(s)	2527
Cac8I	GCNNGC	7 cut(s)	250, 1336, 1609, 1792, 2023, 2226, 2259
CaiI	CAGNNNCTG	3 cut(s)	923, 2216, 2378
CciI	TCATGA	1 cut(s)	843
CfoI	GCGC	3 cut(s)	68, 1077, 2021
CseI	GACGC	2 cut(s)	22, 2511
Csp6I	GTAC	4 cut(s)	588, 875, 1425, 2120
CviAII	CATG	9 cut(s)	169, 605, 844, 1197, 1511, 1787, 1922, 1973, 2138
CviQI	GTAC	4 cut(s)	588, 875, 1425, 2120
DdeI	CTNAG	8 cut(s)	40, 1145, 1260, 1320, 1823, 1938, 2220, 2619
DinI	GGCGCC	1 cut(s)	67
DpnI	GATC	9 cut(s)	451, 719, 1092, 1125, 1195, 1227, 1299, 1959, 2334
DpnII	GATC	9 cut(s)	449, 717, 1090, 1123, 1193, 1225, 1297, 1957, 2332
DraI	TTTAAA	1 cut(s)	2190
DraIII	CACNNNGTG	1 cut(s)	1721
DrdI	GACNNNNNNGTC	2 cut(s)	1118, 2424
DriI	GACNNNNNGTC	1 cut(s)	1399
DseDI	GACNNNNNNGTC	2 cut(s)	1118, 2424
EaeI	YGGCCR	4 cut(s)	1792, 1975, 2235, 2489
Eam1104I	CTCTTC	1 cut(s)	378
Eam1105I	GACNNNNNGTC	1 cut(s)	1399
EarI	CTCTTC	1 cut(s)	378
Ecl136II	GAGCTC	1 cut(s)	2616
Eco130I	CCWWGG	2 cut(s)	1067, 2114
Eco24I	GRGCYC	2 cut(s)	2043, 2618
Eco31I	GGTCTC	1 cut(s)	2509
Eco32I	GATATC	2 cut(s)	333, 439
Eco47I	GGWCC	7 cut(s)	740, 1141, 1283, 1439, 1962, 2133, 2354
Eco47III	AGCGCT	1 cut(s)	2020
Eco53kI	GAGCTC	1 cut(s)	2616
Eco57I	CTGAAG	3 cut(s)	1004, 1018, 1365
Eco88I	CYCGRG	1 cut(s)	430
EcoICRI	GAGCTC	1 cut(s)	2616
EcoNI	CCTNNNNNAGG	2 cut(s)	996, 1148
EcoO109I	RGGNCCY	4 cut(s)	1141, 1693, 1869, 2354
EcoRII	CCWGG	5 cut(s)	544, 1279, 2043, 2237, 2282
EcoRV	GATATC	2 cut(s)	333, 439
EcoT14I	CCWWGG	2 cut(s)	1067, 2114
EcoT22I	ATGCAT	1 cut(s)	1545
EcoT38I	GRGCYC	2 cut(s)	2043, 2618
EgeI	GGCGCC	1 cut(s)	67
EheI	GGCGCC	1 cut(s)	67
ErhI	CCWWGG	2 cut(s)	1067, 2114
FaeI	CATG	9 cut(s)	172, 608, 847, 1200, 1514, 1790, 1925, 1976, 2141
FaqI	GGGAC	7 cut(s)	22, 654, 948, 1425, 1636, 1693, 2119
FatI	CATG	9 cut(s)	168, 604, 843, 1196, 1510, 1786, 1921, 1972, 2137
FbaI	TGATCA	1 cut(s)	1090
FblI	GTMKAC	1 cut(s)	1239
FokI	GGATG	4 cut(s)	372, 1280, 1894, 2578
FriOI	GRGCYC	2 cut(s)	2043, 2618
GlaI	GCGC	3 cut(s)	67, 1076, 2020
GsuI	CTGGAG	4 cut(s)	855, 1425, 2027, 2328
HaeII	RGCGCY	3 cut(s)	69, 1078, 2022
HapII	CCGG	5 cut(s)	6, 69, 239, 1503, 1656
HgaI	GACGC	2 cut(s)	22, 2511
HhaI	GCGC	3 cut(s)	68, 1077, 2021
Hin1I	GRCGYC	2 cut(s)	66, 165
Hin1II	CATG	9 cut(s)	172, 608, 847, 1200, 1514, 1790, 1925, 1976, 2141
Hin6I	GCGC	3 cut(s)	66, 1075, 2019
HinP1I	GCGC	3 cut(s)	66, 1075, 2019
HincII	GTYRAC	1 cut(s)	475
HindII	GTYRAC	1 cut(s)	475
HindIII	AAGCTT	1 cut(s)	970
HinfI	GANTC	9 cut(s)	108, 520, 981, 1507, 1675, 1830, 1879, 2089, 2393
HpaII	CCGG	5 cut(s)	6, 69, 239, 1503, 1656
HphI	GGTGA	4 cut(s)	103, 173, 2241, 2388
Hpy166II	GTNNAC	8 cut(s)	97, 475, 682, 1240, 1411, 1573, 2052, 2156
Hpy8I	GTNNAC	8 cut(s)	97, 475, 682, 1240, 1411, 1573, 2052, 2156
Hpy99I	CGWCG	4 cut(s)	16, 122, 302, 2340
HpyAV	CCTTC	9 cut(s)	112, 238, 367, 850, 915, 929, 1195, 1467, 2550
HpyCH4III	ACNGT	5 cut(s)	458, 747, 1581, 2133, 2212
HpyCH4IV	ACGT	8 cut(s)	141, 165, 300, 503, 561, 1427, 1569, 2338
HpyF10VI	GCNNNNNNNGC	7 cut(s)	22, 1691, 1700, 2225, 2234, 2462, 2519
HpyF3I	CTNAG	8 cut(s)	40, 1145, 1260, 1320, 1823, 1938, 2220, 2619
HpySE526I	ACGT	8 cut(s)	141, 165, 300, 503, 561, 1427, 1569, 2338
Hsp92I	GRCGYC	2 cut(s)	66, 165
Hsp92II	CATG	9 cut(s)	172, 608, 847, 1200, 1514, 1790, 1925, 1976, 2141
HspAI	GCGC	3 cut(s)	66, 1075, 2019
KasI	GGCGCC	1 cut(s)	65
Ksp22I	TGATCA	1 cut(s)	1090
Kzo9I	GATC	9 cut(s)	449, 717, 1090, 1123, 1193, 1225, 1297, 1957, 2332
LguI	GCTCTTC	1 cut(s)	378
LmnI	GCTCC	3 cut(s)	135, 1765, 2046
Lsp1109I	GCAGC	7 cut(s)	298, 1064, 1647, 1930, 2203, 2215, 2452
LweI	GCATC	3 cut(s)	858, 863, 1462
MaeII	ACGT	8 cut(s)	141, 165, 300, 503, 561, 1427, 1569, 2338
MaeIII	GTNAC	6 cut(s)	35, 109, 161, 760, 818, 2523
MalI	GATC	9 cut(s)	451, 719, 1092, 1125, 1195, 1227, 1299, 1959, 2334
MbiI	CCGCTC	1 cut(s)	127
MboI	GATC	9 cut(s)	449, 717, 1090, 1123, 1193, 1225, 1297, 1957, 2332
MfeI	CAATTG	2 cut(s)	861, 2597
MflI	RGATCY	2 cut(s)	449, 1297
MhlI	GDGCHC	4 cut(s)	1951, 2043, 2158, 2618
MlsI	TGGCCA	3 cut(s)	1794, 1977, 2237
MluNI	TGGCCA	3 cut(s)	1794, 1977, 2237
Mly113I	GGCGCC	1 cut(s)	66
MlyI	GAGTC	5 cut(s)	117, 514, 1516, 1669, 2083
MmeI	TCCRAC	5 cut(s)	524, 1188, 1703, 1969, 2143
Mox20I	TGGCCA	3 cut(s)	1794, 1977, 2237
Mph1103I	ATGCAT	1 cut(s)	1545
MroXI	GAANNNNTTC	3 cut(s)	101, 612, 2477
MscI	TGGCCA	3 cut(s)	1794, 1977, 2237
MseI	TTAA	9 cut(s)	540, 774, 957, 996, 1137, 1491, 2079, 2169, 2189
MslI	CAYNNNNRTG	1 cut(s)	1049
Msp20I	TGGCCA	3 cut(s)	1794, 1977, 2237
MspA1I	CMGCKG	1 cut(s)	2582
MspI	CCGG	5 cut(s)	6, 69, 239, 1503, 1656
MspR9I	CCNGG	9 cut(s)	7, 70, 546, 1281, 1504, 1657, 2045, 2239, 2284
MunI	CAATTG	2 cut(s)	861, 2597
Mva1269I	GAATGC	3 cut(s)	349, 774, 2436
MvaI	CCWGG	5 cut(s)	546, 1281, 2045, 2239, 2284
MvnI	CGCG	2 cut(s)	159, 979
MwoI	GCNNNNNNNGC	7 cut(s)	22, 1691, 1700, 2225, 2234, 2462, 2519
NarI	GGCGCC	1 cut(s)	66
NciI	CCSGG	4 cut(s)	7, 70, 1504, 1657
NdeII	GATC	9 cut(s)	449, 717, 1090, 1123, 1193, 1225, 1297, 1957, 2332
NheI	GCTAGC	2 cut(s)	1607, 2021
NlaIII	CATG	9 cut(s)	172, 608, 847, 1200, 1514, 1790, 1925, 1976, 2141
NlaIV	GGNNCC	8 cut(s)	67, 131, 237, 1441, 1871, 2001, 2042, 2135
NmeAIII	GCCGAG	2 cut(s)	62, 148
NmuCI	GTSAC	5 cut(s)	109, 161, 760, 818, 2523
NsiI	ATGCAT	1 cut(s)	1545
PagI	TCATGA	1 cut(s)	843
PciSI	GCTCTTC	1 cut(s)	378
PctI	GAATGC	3 cut(s)	349, 774, 2436
PdmI	GAANNNNTTC	3 cut(s)	101, 612, 2477
PfeI	GAWTC	4 cut(s)	981, 1830, 1879, 2393
PleI	GAGTC	5 cut(s)	116, 514, 1515, 1669, 2083
PluTI	GGCGCC	1 cut(s)	69
PpsI	GAGTC	5 cut(s)	116, 514, 1515, 1669, 2083
Ppu21I	YACGTR	2 cut(s)	562, 1570
PpuMI	RGGWCCY	2 cut(s)	1141, 2354
PshAI	GACNNNNGTC	1 cut(s)	2521
PshBI	ATTAAT	1 cut(s)	2169
PsiI	TTATAA	1 cut(s)	665
Psp124BI	GAGCTC	1 cut(s)	2618
Psp1406I	AACGTT	1 cut(s)	503
Psp5II	RGGWCCY	2 cut(s)	1141, 2354
Psp6I	CCWGG	5 cut(s)	544, 1279, 2043, 2237, 2282
PspGI	CCWGG	5 cut(s)	544, 1279, 2043, 2237, 2282
PspN4I	GGNNCC	8 cut(s)	67, 131, 237, 1441, 1871, 2001, 2042, 2135
PspPPI	RGGWCCY	2 cut(s)	1141, 2354
PstNI	CAGNNNCTG	3 cut(s)	923, 2216, 2378
PsuI	RGATCY	2 cut(s)	449, 1297
PvuII	CAGCTG	1 cut(s)	2582
RsaI	GTAC	4 cut(s)	589, 876, 1426, 2121
RsaNI	GTAC	4 cut(s)	588, 875, 1425, 2120
RseI	CAYNNNNRTG	1 cut(s)	1049
SacI	GAGCTC	1 cut(s)	2618
SapI	GCTCTTC	1 cut(s)	378
SaqAI	TTAA	9 cut(s)	540, 774, 957, 996, 1137, 1491, 2079, 2169, 2189
Sau3AI	GATC	9 cut(s)	449, 717, 1090, 1123, 1193, 1225, 1297, 1957, 2332
ScaI	AGTACT	1 cut(s)	876
SchI	GAGTC	5 cut(s)	117, 514, 1516, 1669, 2083
ScrFI	CCNGG	9 cut(s)	7, 70, 546, 1281, 1504, 1657, 2045, 2239, 2284
SduI	GDGCHC	4 cut(s)	1951, 2043, 2158, 2618
SfaNI	GCATC	3 cut(s)	858, 863, 1462
SfcI	CTRYAG	2 cut(s)	1185, 1834
SfoI	GGCGCC	1 cut(s)	67
SinI	GGWCC	7 cut(s)	740, 1141, 1283, 1439, 1962, 2133, 2354
SmiMI	CAYNNNNRTG	1 cut(s)	1049
SmlI	CTYRAG	2 cut(s)	1380, 1927
SmoI	CTYRAG	2 cut(s)	1380, 1927
SpeI	ACTAGT	1 cut(s)	2639
SsiI	CCGC	6 cut(s)	23, 60, 63, 114, 127, 159
SspDI	GGCGCC	1 cut(s)	65
SstI	GAGCTC	1 cut(s)	2618
StyD4I	CCNGG	9 cut(s)	5, 68, 544, 1279, 1502, 1655, 2043, 2237, 2282
StyI	CCWWGG	2 cut(s)	1067, 2114
TaaI	ACNGT	5 cut(s)	458, 747, 1581, 2133, 2212
TaiI	ACGT	8 cut(s)	144, 168, 303, 506, 564, 1430, 1572, 2341
TaqI	TCGA	9 cut(s)	534, 645, 1029, 1122, 1495, 1601, 2084, 2335, 2428
TaqII	GACCGA	1 cut(s)	1950
TatI	WGTACW	2 cut(s)	587, 874
TauI	GCSGC	3 cut(s)	63, 66, 130
TfiI	GAWTC	4 cut(s)	981, 1830, 1879, 2393
Tru1I	TTAA	9 cut(s)	540, 774, 957, 996, 1137, 1491, 2079, 2169, 2189
Tru9I	TTAA	9 cut(s)	540, 774, 957, 996, 1137, 1491, 2079, 2169, 2189
TscAI	CASTG	5 cut(s)	1028, 1381, 1525, 1831, 2217
TseFI	GTSAC	5 cut(s)	109, 161, 760, 818, 2523
TseI	GCWGC	7 cut(s)	311, 1077, 1660, 1918, 2216, 2228, 2465
Tsp45I	GTSAC	5 cut(s)	109, 161, 760, 818, 2523
TspDTI	ATGAA	7 cut(s)	621, 832, 1067, 1284, 1892, 1896, 2470
TspGWI	ACGGA	1 cut(s)	1972
TspRI	CASTG	5 cut(s)	1028, 1381, 1525, 1831, 2217
VneI	GTGCAC	1 cut(s)	2154
VpaK11BI	GGWCC	7 cut(s)	740, 1141, 1283, 1439, 1962, 2133, 2354
VspI	ATTAAT	1 cut(s)	2169
XagI	CCTNNNNNAGG	2 cut(s)	996, 1148
XapI	RAATTY	2 cut(s)	612, 1597
XcmI	CCANNNNNNNNNTGG	2 cut(s)	146, 1985
XmiI	GTMKAC	1 cut(s)	1239
XmnI	GAANNNNTTC	3 cut(s)	101, 612, 2477
ZraI	GACGTC	1 cut(s)	166
ZrmI	AGTACT	1 cut(s)	876
Zsp2I	ATGCAT	1 cut(s)	1545

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.