FvH4_5g04360

Protein of unknown function (DUF3593)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011911
Physical Location & Seq
Forward (+)
2573242 .. 2574789
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04360.t2

Sequence Viewer

Length: 564 bp
ATGACTATTACTTGTGTTCAGTTTCATTGCAAAGTTCCCAACTTTGTTATCCCTCCACATTTTCTGGGTTCCTCTTCTTTCTTGCAAAGTCCCCAACCGAACCCACCACTAAGAAACAGAAACCCCAAGAGTTACACACAGAGAAGTGGAGACATAAGAGGTGGGATTGTCTGTGGGGTGATGTTTCCAGTTGACCCATGGGCACCCAACATTGATTCACAGAGCATAGCTTCACAGCTGTTTGCTGCTTCTTTGTTTCCGTATCTTGGTTTCTTGTACTTCCTCACCAAGTCCAAGACTGCCCCAAAGCTCACCCTTTTTGGGTTTTACTTCTTGCTAGCCTTTGTTGGGGCAACCATCCCTGCAGGAATTTATGCAAAGGTGAACTACAGCACTTCCTTGGCGAATGTTGATTGGTTACATGGTGGGGCTGAGTCGCTTCTCACTCTAACCAACATATTCATTGTGTTGGGGTTGAGAGAAGCTCTGAGGGAAGCTAAAGCAAAAGAAGGAACGTCAGATCCTATTCCAGGGTTGAAAGAGGAAAAGAAAACTTCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3593

Protein Analysis

188

Amino Acids

20.47

Weight (kDa)

9.32

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3593 PF12159 78 - 119 2.8e-13 Protein of unknown function (DUF3593)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 202
AclWI GGATC 1 cut(s) 515
AcsI RAATTY 1 cut(s) 369
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 4 cut(s) 266, 321, 348, 530
AgsI TTSAA 2 cut(s) 538, 558
AjnI CCWGG 1 cut(s) 529
AloI GAACNNNNNNTCC 2 cut(s) 505, 537
AluBI AGCT 5 cut(s) 230, 238, 310, 485, 497
AluI AGCT 5 cut(s) 230, 238, 310, 485, 497
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 1 cut(s) 515
ApeKI GCWGC 1 cut(s) 245
ApoI RAATTY 1 cut(s) 369
AsuHPI GGTGA 4 cut(s) 190, 277, 304, 394
AsuNHI GCTAGC 1 cut(s) 337
BaeGI GKGCMC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 365
BciT130I CCWGG 1 cut(s) 531
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 2 cut(s) 363, 388
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
Bme1390I CCNGG 1 cut(s) 531
BmiI GGNNCC 2 cut(s) 70, 204
BmrFI CCNGG 1 cut(s) 531
BmtI GCTAGC 1 cut(s) 341
BplI GAGNNNNNCTC 2 cut(s) 469, 501
BsaJI CCNNGG 3 cut(s) 197, 399, 530
Bsc4I CCNNNNNNNGG 4 cut(s) 266, 321, 348, 530
Bse1I ACTGG 1 cut(s) 188
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 3 cut(s) 197, 399, 530
BseGI GGATG 1 cut(s) 357
BseLI CCNNNNNNNGG 4 cut(s) 266, 321, 348, 530
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 2 cut(s) 423, 479
BseNI ACTGG 1 cut(s) 188
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 1 cut(s) 232
BshNI GGYRCC 1 cut(s) 202
BslFI GGGAC 1 cut(s) 75
BslI CCNNNNNNNGG 4 cut(s) 266, 321, 348, 530
BsmAI GTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 205
Bsp143I GATC 1 cut(s) 520
Bsp19I CCATGG 1 cut(s) 197
BspCNI CTCAG 2 cut(s) 424, 480
BspLI GGNNCC 2 cut(s) 70, 204
BspMAI CTGCAG 1 cut(s) 367
BspOI GCTAGC 1 cut(s) 341
BspPI GGATC 1 cut(s) 515
BspT107I GGYRCC 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 1 cut(s) 188
BssECI CCNNGG 3 cut(s) 197, 399, 530
BssMI GATC 1 cut(s) 520
BssT1I CCWWGG 2 cut(s) 197, 399
Bst2UI CCWGG 1 cut(s) 531
Bst6I CTCTTC 1 cut(s) 79
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 3 cut(s) 110, 432, 488
BstDSI CCRYGG 1 cut(s) 197
BstENI CCTNNNNNAGG 1 cut(s) 528
BstF5I GGATG 1 cut(s) 357
BstKTI GATC 1 cut(s) 523
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 1 cut(s) 520
BstNI CCWGG 1 cut(s) 531
BstSCI CCNGG 1 cut(s) 529
BstSFI CTRYAG 2 cut(s) 363, 388
BstSLI GKGCMC 1 cut(s) 205
BstV1I GCAGC 1 cut(s) 232
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BtgI CCRYGG 1 cut(s) 197
BtsCI GGATG 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 339
Csp6I GTAC 1 cut(s) 277
CviAII CATG 2 cut(s) 198, 422
CviJI RGCY 7 cut(s) 230, 238, 310, 341, 431, 485, 497
CviKI_1 RGCY 7 cut(s) 230, 238, 310, 341, 431, 485, 497
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 3 cut(s) 110, 432, 488
DpnI GATC 1 cut(s) 522
DpnII GATC 1 cut(s) 520
Eam1104I CTCTTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 79
Eco130I CCWWGG 2 cut(s) 197, 399
EcoNI CCTNNNNNAGG 1 cut(s) 528
EcoRII CCWGG 1 cut(s) 529
EcoT14I CCWWGG 2 cut(s) 197, 399
ErhI CCWWGG 2 cut(s) 197, 399
FaeI CATG 2 cut(s) 201, 425
FaiI YATR 6 cut(s) 155, 199, 227, 375, 423, 458
FaqI GGGAC 1 cut(s) 75
FatI CATG 2 cut(s) 197, 421
Fnu4HI GCNGC 1 cut(s) 246
FokI GGATG 1 cut(s) 344
Fsp4HI GCNGC 1 cut(s) 246
FspBI CTAG 1 cut(s) 338
GluI GCNGC 1 cut(s) 246
Hin1II CATG 2 cut(s) 201, 425
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 2 cut(s) 215, 434
HphI GGTGA 4 cut(s) 190, 277, 304, 394
Hpy166II GTNNAC 2 cut(s) 193, 385
Hpy188I TCNGA 2 cut(s) 489, 520
Hpy8I GTNNAC 2 cut(s) 193, 385
HpyAV CCTTC 1 cut(s) 503
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 4 cut(s) 30, 85, 365, 377
HpyF3I CTNAG 3 cut(s) 110, 432, 488
HpySE526I ACGT 1 cut(s) 515
Hsp92II CATG 2 cut(s) 201, 425
Kzo9I GATC 1 cut(s) 520
LpnPI CCDG 6 cut(s) 50, 201, 351, 375, 516, 543
Lsp1109I GCAGC 1 cut(s) 232
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 2 cut(s) 131, 417
MalI GATC 1 cut(s) 522
MboI GATC 1 cut(s) 520
MboII GAAGA 1 cut(s) 66
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 1 cut(s) 205
MluCI AATT 2 cut(s) 369, 558
MlyI GAGTC 1 cut(s) 443
MnlI CCTC 6 cut(s) 63, 82, 152, 293, 483, 535
MspA1I CMGCKG 1 cut(s) 238
MspR9I CCNGG 1 cut(s) 531
MvaI CCWGG 1 cut(s) 531
NcoI CCATGG 1 cut(s) 197
NdeII GATC 1 cut(s) 520
NheI GCTAGC 1 cut(s) 337
NlaIII CATG 2 cut(s) 201, 425
NlaIV GGNNCC 2 cut(s) 70, 204
PfeI GAWTC 1 cut(s) 215
PkrI GCNGC 1 cut(s) 247
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 2 cut(s) 70, 204
PstI CTGCAG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 520
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 1 cut(s) 520
SbfI CCTGCAGG 1 cut(s) 367
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 1 cut(s) 531
SdaI CCTGCAGG 1 cut(s) 367
SduI GDGCHC 1 cut(s) 205
SetI ASST 8 cut(s) 163, 232, 240, 312, 384, 487, 499, 518
SfcI CTRYAG 2 cut(s) 363, 388
Sse8387I CCTGCAGG 1 cut(s) 367
Sse9I AATT 2 cut(s) 369, 558
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 1 cut(s) 529
StyI CCWWGG 2 cut(s) 197, 399
TaiI ACGT 1 cut(s) 518
TasI AATT 2 cut(s) 369, 558
TatI WGTACW 1 cut(s) 276
TfiI GAWTC 1 cut(s) 215
TseI GCWGC 1 cut(s) 245
TspDTI ATGAA 2 cut(s) 14, 451
TspGWI ACGGA 1 cut(s) 249
XagI CCTNNNNNAGG 1 cut(s) 528
XapI RAATTY 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 195
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.