MD14G1122900.v1.1

Protein of unknown function (DUF3593)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011911
Physical Location & Seq
Forward (+)
19735202 .. 19736600
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1122900.v1.1.491

Sequence Viewer

Length: 585 bp
ATGACTATCACCTGCATTCAATCTCTCTGTAAATCCCCCGCCTTTCTTCTCCCTCCTACTCTTCTGGGTTCCTTTTGTTTCTCTAGGAGCCCCGAACCGAACCCATTTTCCAGAAACAGAGAATCCAAGTTTTACACAGAGAGAAGGATCGGCAGTAAAAACAGAAGAGGTGGGGGTGGGATTGTCTGTGGGGTTGTGGTATTCCCAATTGATCCATGGGCGCCCAGCATTGATTCACAGAGCATAGCTTCCCAGCTGTTTGCAGCTTCTCTGTTTCCCTACCTGGGTTTCTTGTACTTCATCACCAAATCCAAGTCTGCCCCGAAGCTCACCCTCTTCGGGTTCTACTTCTTGCTTGCCTTTGTGGGGGCCACCATTCCTGCTGGAATTTATGCAAAGGTGAACTATGGCACTTCATTGGCGAATGTCGATTGGTTACATGGTGGGGCTGAGGCACTTCTCACTCTGACCAACATTTTTATTGTGTTGGGGTTGAGAGGAGCTCTTAGAAAAGCTGAGGATGCAGAAGAAAGAACATCAACTCCTATTCCCGGGTTGAAGGACGAGACCAAAACTTCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3593

Protein Analysis

195

Amino Acids

21.05

Weight (kDa)

9.39

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3593 PF12159 84 - 169 7.6e-39 Protein of unknown function (DUF3593)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 20
Acc36I ACCTGC 1 cut(s) 20
AccB1I GGYRCC 1 cut(s) 220
AciI CCGC 1 cut(s) 39
AclWI GGATC 2 cut(s) 155, 206
AcsI RAATTY 1 cut(s) 387
AcyI GRCGYC 1 cut(s) 221
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 4 cut(s) 284, 339, 366, 551
AgsI TTSAA 2 cut(s) 20, 559
AjnI CCWGG 1 cut(s) 282
AloI GAACNNNNNNTCC 4 cut(s) 92, 124, 526, 558
AluBI AGCT 6 cut(s) 248, 256, 266, 328, 503, 515
AluI AGCT 6 cut(s) 248, 256, 266, 328, 503, 515
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 2 cut(s) 155, 206
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 1 cut(s) 369
ApeKI GCWGC 1 cut(s) 263
ApoI RAATTY 1 cut(s) 387
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 1 cut(s) 369
AsuC2I CCSGG 2 cut(s) 552, 553
AsuHPI GGTGA 3 cut(s) 295, 322, 412
AvaI CYCGRG 1 cut(s) 551
BanI GGYRCC 1 cut(s) 220
BanII GRGCYC 2 cut(s) 92, 505
Bbv12I GWGCWC 1 cut(s) 505
BbvCI CCTCAGC 2 cut(s) 450, 516
BbvI GCAGC 1 cut(s) 275
BciT130I CCWGG 1 cut(s) 284
BcnI CCSGG 2 cut(s) 552, 553
BcoDI GTCTC 1 cut(s) 560
BfaI CTAG 1 cut(s) 84
BfoI RGCGCY 1 cut(s) 224
BfuAI ACCTGC 1 cut(s) 20
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
Bme1390I CCNGG 3 cut(s) 284, 552, 553
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 4 cut(s) 70, 89, 222, 370
BmrFI CCNGG 3 cut(s) 284, 552, 553
BmsI GCATC 1 cut(s) 511
BplI GAGNNNNNCTC 2 cut(s) 487, 519
Bpu10I CCTNAGC 2 cut(s) 450, 516
BpuMI CCSGG 2 cut(s) 552, 553
BsaHI GRCGYC 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 3 cut(s) 215, 283, 551
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 4 cut(s) 284, 339, 366, 551
BseBI CCWGG 1 cut(s) 284
BseDI CCNNGG 3 cut(s) 215, 283, 551
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 4 cut(s) 284, 339, 366, 551
BseMII CTCAG 2 cut(s) 441, 507
BseRI GAGGAG 1 cut(s) 513
BseXI GCAGC 1 cut(s) 275
BseYI CCCAGC 2 cut(s) 224, 252
BshFI GGCC 1 cut(s) 371
BshNI GGYRCC 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 505
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 1 cut(s) 552
BslI CCNNNNNNNGG 4 cut(s) 284, 339, 366, 551
BsmAI GTCTC 1 cut(s) 560
BsmI GAATGC 1 cut(s) 15
BsnI GGCC 1 cut(s) 371
Bso31I GGTCTC 1 cut(s) 560
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 2 cut(s) 92, 505
Bsp143I GATC 2 cut(s) 147, 211
Bsp19I CCATGG 1 cut(s) 215
BspACI CCGC 1 cut(s) 39
BspANI GGCC 1 cut(s) 371
BspCNI CTCAG 2 cut(s) 442, 508
BspLI GGNNCC 4 cut(s) 70, 89, 222, 370
BspMI ACCTGC 1 cut(s) 20
BspPI GGATC 2 cut(s) 155, 206
BspT107I GGYRCC 1 cut(s) 220
BspTNI GGTCTC 1 cut(s) 560
BssECI CCNNGG 3 cut(s) 215, 283, 551
BssMI GATC 2 cut(s) 147, 211
BssNI GRCGYC 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 284
Bst6I CTCTTC 3 cut(s) 66, 160, 341
BstACI GRCGYC 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 357
BstDEI CTNAG 4 cut(s) 450, 506, 516, 582
BstDSI CCRYGG 1 cut(s) 215
BstF5I GGATG 1 cut(s) 526
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 2 cut(s) 150, 214
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 2 cut(s) 147, 211
BstMWI GCNNNNNNNGC 1 cut(s) 521
BstNI CCWGG 1 cut(s) 284
BstSCI CCNGG 3 cut(s) 282, 550, 551
BstV1I GCAGC 1 cut(s) 275
BsuRI GGCC 1 cut(s) 371
BtgI CCRYGG 1 cut(s) 215
BtsCI GGATG 1 cut(s) 526
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 357
CfoI GCGC 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 369
Cfr9I CCCGGG 1 cut(s) 551
Csp6I GTAC 1 cut(s) 295
CviAII CATG 2 cut(s) 216, 440
CviJI RGCY 9 cut(s) 90, 248, 256, 266, 328, 371, 449, 503, 515
CviKI_1 RGCY 9 cut(s) 90, 248, 256, 266, 328, 371, 449, 503, 515
CviQI GTAC 1 cut(s) 295
DdeI CTNAG 4 cut(s) 450, 506, 516, 582
DinI GGCGCC 1 cut(s) 222
DpnI GATC 2 cut(s) 149, 213
DpnII GATC 2 cut(s) 147, 211
Eam1104I CTCTTC 3 cut(s) 66, 160, 341
EarI CTCTTC 3 cut(s) 66, 160, 341
Ecl136II GAGCTC 1 cut(s) 503
Eco130I CCWWGG 1 cut(s) 215
Eco24I GRGCYC 2 cut(s) 92, 505
Eco31I GGTCTC 1 cut(s) 560
Eco53kI GAGCTC 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 551
EcoICRI GAGCTC 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 215
EcoT38I GRGCYC 2 cut(s) 92, 505
EgeI GGCGCC 1 cut(s) 222
EheI GGCGCC 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 215
FaeI CATG 2 cut(s) 219, 443
FaiI YATR 5 cut(s) 217, 245, 393, 408, 441
FatI CATG 2 cut(s) 215, 439
FauI CCCGC 1 cut(s) 46
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 2 cut(s) 92, 505
Fsp4HI GCNGC 1 cut(s) 264
FspBI CTAG 1 cut(s) 84
GlaI GCGC 1 cut(s) 222
GluI GCNGC 1 cut(s) 264
GsaI CCCAGC 2 cut(s) 228, 256
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 1 cut(s) 371
HapII CCGG 1 cut(s) 552
HhaI GCGC 1 cut(s) 223
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 2 cut(s) 219, 443
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HinfI GANTC 2 cut(s) 122, 233
HpaII CCGG 1 cut(s) 552
HphI GGTGA 3 cut(s) 295, 322, 412
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 1 cut(s) 468
Hpy188III TCNNGA 1 cut(s) 111
Hpy8I GTNNAC 1 cut(s) 403
HpyAV CCTTC 2 cut(s) 138, 553
HpyCH4V TGCA 4 cut(s) 15, 263, 395, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 521
HpyF3I CTNAG 4 cut(s) 450, 506, 516, 582
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 2 cut(s) 219, 443
HspAI GCGC 1 cut(s) 221
KasI GGCGCC 1 cut(s) 220
Kzo9I GATC 2 cut(s) 147, 211
LmnI GCTCC 2 cut(s) 87, 500
Lsp1109I GCAGC 1 cut(s) 275
LweI GCATC 1 cut(s) 511
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 435
MalI GATC 2 cut(s) 149, 213
MboI GATC 2 cut(s) 147, 211
MboII GAAGA 5 cut(s) 38, 53, 177, 328, 539
MfeI CAATTG 1 cut(s) 207
MhlI GDGCHC 2 cut(s) 92, 505
MluCI AATT 2 cut(s) 207, 387
Mly113I GGCGCC 1 cut(s) 221
MnlI CCTC 6 cut(s) 63, 161, 344, 445, 491, 511
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 1 cut(s) 552
MspR9I CCNGG 3 cut(s) 284, 552, 553
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 521
NarI GGCGCC 1 cut(s) 221
NciI CCSGG 2 cut(s) 552, 553
NcoI CCATGG 1 cut(s) 215
NdeII GATC 2 cut(s) 147, 211
NlaIII CATG 2 cut(s) 219, 443
NlaIV GGNNCC 4 cut(s) 70, 89, 222, 370
PaqCI CACCTGC 1 cut(s) 20
PctI GAATGC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 122, 233
PkrI GCNGC 1 cut(s) 265
PluTI GGCGCC 1 cut(s) 224
Psp124BI GAGCTC 1 cut(s) 505
Psp6I CCWGG 1 cut(s) 282
PspFI CCCAGC 2 cut(s) 224, 252
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 4 cut(s) 70, 89, 222, 370
PspPI GGNCC 1 cut(s) 369
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
SacI GAGCTC 1 cut(s) 505
SatI GCNGC 1 cut(s) 264
Sau3AI GATC 2 cut(s) 147, 211
Sau96I GGNCC 1 cut(s) 369
ScrFI CCNGG 3 cut(s) 284, 552, 553
SduI GDGCHC 2 cut(s) 92, 505
SfaNI GCATC 1 cut(s) 511
SfoI GGCGCC 1 cut(s) 222
SmaI CCCGGG 1 cut(s) 553
Sse9I AATT 2 cut(s) 207, 387
SsiI CCGC 1 cut(s) 39
SspDI GGCGCC 1 cut(s) 220
SspMI CTAG 1 cut(s) 84
SstI GAGCTC 1 cut(s) 505
StyD4I CCNGG 3 cut(s) 282, 550, 551
StyI CCWWGG 1 cut(s) 215
TaqI TCGA 1 cut(s) 429
TasI AATT 2 cut(s) 207, 387
TatI WGTACW 1 cut(s) 294
TfiI GAWTC 2 cut(s) 122, 233
TseI GCWGC 1 cut(s) 263
TspDTI ATGAA 2 cut(s) 289, 405
TspMI CCCGGG 1 cut(s) 551
XapI RAATTY 1 cut(s) 387
XcmI CCANNNNNNNNNTGG 1 cut(s) 213
XmaI CCCGGG 1 cut(s) 551
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.