MD06G1104000.v1.1

Protein of unknown function (DUF3593)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011911
Physical Location & Seq
Forward (+)
24216908 .. 24218314
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1104000.v1.1.491

Sequence Viewer

Length: 582 bp
ATGACCATCACCTGCATTCAATCTCTCTGTAAATCCCCCACCTTTCTCCCCCCTCCAACTTCTCTGGGTTCCCCATGTTTCTCGCGGATCCCCGACCCGAACCCACTTTCCAGAAACAGAGAATCCAAGTTTTACACAGAGAGGAGAATCGGCAGTAAAAGCAGAAGAGGTTGGGGTGGGATTGTGTGTGGTGTGGCGTTCCCGATTGATCCATGGGCGCCCAGCATTGACTCACAGAGCATAGCTTCACAGCTGTTTGCAGCTTCTCTGTTTCCCTACCTGGGTTTCTTGTACTTCATCACCAAGTCAAAGTCTGCCCCAAAGCTCACTCTTTTTGGGTTCTACTTCTTGCTTGCTTTTGTGGGGGCCACCATTCCTGCTGGAATTTATGCAAAGGTGAATTACGGCACTTCATTGGCGAATGTCGATTGGTTACATGGCGGGGCTGAGGCATTTCTCACTCTAACCAACATATGCATCGTGTTGGGGTTGGGGGGGGCTCTCAGAAAAGCTGGAGATGCAGAAGAAAGAACATCAGCTCCTATTTCCGGGTTGAAGGAGGAGAAGAAAACTTCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3593

Protein Analysis

194

Amino Acids

20.79

Weight (kDa)

9.4

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3593 PF12159 83 - 167 2.2e-38 Protein of unknown function (DUF3593)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 20
Acc36I ACCTGC 1 cut(s) 20
AccB1I GGYRCC 1 cut(s) 217
AccII CGCG 1 cut(s) 85
AciI CCGC 2 cut(s) 85, 441
AclWI GGATC 3 cut(s) 82, 95, 203
AcsI RAATTY 1 cut(s) 384
AcyI GRCGYC 1 cut(s) 218
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 2 cut(s) 281, 548
AgsI TTSAA 2 cut(s) 20, 556
AjnI CCWGG 1 cut(s) 279
AloI GAACNNNNNNTCC 4 cut(s) 92, 124, 523, 555
AluBI AGCT 6 cut(s) 245, 253, 263, 325, 512, 539
AluI AGCT 6 cut(s) 245, 253, 263, 325, 512, 539
AlwI GGATC 3 cut(s) 82, 95, 203
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 1 cut(s) 384
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 1 cut(s) 366
AsuC2I CCSGG 1 cut(s) 550
AsuHPI GGTGA 2 cut(s) 292, 409
BamHI GGATCC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 502
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 272
BccI CCATC 1 cut(s) 14
BceAI ACGGC 1 cut(s) 421
BciT130I CCWGG 1 cut(s) 281
BcnI CCSGG 1 cut(s) 550
BfoI RGCGCY 1 cut(s) 221
BfuAI ACCTGC 1 cut(s) 20
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 281, 550
BmgT120I GGNCC 1 cut(s) 366
BmiI GGNNCC 4 cut(s) 70, 89, 219, 367
BmrFI CCNGG 2 cut(s) 281, 550
BmsI GCATC 2 cut(s) 486, 508
BpmI CTGGAG 1 cut(s) 534
Bpu10I CCTNAGC 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 550
BsaHI GRCGYC 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 212, 280
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 548
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 212, 280
BseLI CCNNNNNNNGG 2 cut(s) 281, 548
BseMII CTCAG 2 cut(s) 438, 517
BseRI GAGGAG 2 cut(s) 157, 575
BseXI GCAGC 1 cut(s) 272
BseYI CCCAGC 1 cut(s) 221
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 1 cut(s) 368
BshNI GGYRCC 1 cut(s) 217
BsiSI CCGG 1 cut(s) 549
BslI CCNNNNNNNGG 2 cut(s) 281, 548
BsmI GAATGC 1 cut(s) 15
BsnI GGCC 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 502
Bsp143I GATC 2 cut(s) 87, 208
Bsp19I CCATGG 1 cut(s) 212
BspACI CCGC 2 cut(s) 85, 441
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 2 cut(s) 439, 516
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 4 cut(s) 70, 89, 219, 367
BspMI ACCTGC 1 cut(s) 20
BspPI GGATC 3 cut(s) 82, 95, 203
BspT107I GGYRCC 1 cut(s) 217
BssECI CCNNGG 2 cut(s) 212, 280
BssMI GATC 2 cut(s) 87, 208
BssNI GRCGYC 1 cut(s) 218
BssT1I CCWWGG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 281
Bst6I CTCTTC 1 cut(s) 160
BstACI GRCGYC 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 2 cut(s) 447, 503
BstDSI CCRYGG 1 cut(s) 212
BstFNI CGCG 1 cut(s) 85
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 2 cut(s) 90, 211
BstMBI GATC 2 cut(s) 87, 208
BstMWI GCNNNNNNNGC 2 cut(s) 159, 518
BstNI CCWGG 1 cut(s) 281
BstSCI CCNGG 2 cut(s) 279, 548
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 272
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BsuRI GGCC 1 cut(s) 368
BtgI CCRYGG 1 cut(s) 212
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 354
CfoI GCGC 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 366
Csp6I GTAC 1 cut(s) 292
CviAII CATG 3 cut(s) 75, 213, 437
CviJI RGCY 9 cut(s) 245, 253, 263, 325, 368, 446, 500, 512, 539
CviKI_1 RGCY 9 cut(s) 245, 253, 263, 325, 368, 446, 500, 512, 539
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 2 cut(s) 447, 503
DinI GGCGCC 1 cut(s) 219
DpnI GATC 2 cut(s) 89, 210
DpnII GATC 2 cut(s) 87, 208
Eam1104I CTCTTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 212
Eco24I GRGCYC 1 cut(s) 502
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 212
EcoT22I ATGCAT 1 cut(s) 479
EcoT38I GRGCYC 1 cut(s) 502
EgeI GGCGCC 1 cut(s) 219
EheI GGCGCC 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 212
FaeI CATG 3 cut(s) 78, 216, 440
FaiI YATR 7 cut(s) 76, 214, 242, 390, 438, 473, 475
FatI CATG 3 cut(s) 74, 212, 436
FauI CCCGC 1 cut(s) 434
FauNDI CATATG 1 cut(s) 473
Fnu4HI GCNGC 1 cut(s) 261
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 1 cut(s) 261
GlaI GCGC 1 cut(s) 219
GluI GCNGC 1 cut(s) 261
GsaI CCCAGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 534
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 1 cut(s) 368
HapII CCGG 1 cut(s) 549
HhaI GCGC 1 cut(s) 220
Hin1I GRCGYC 1 cut(s) 218
Hin1II CATG 3 cut(s) 78, 216, 440
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HinfI GANTC 3 cut(s) 122, 147, 230
HpaII CCGG 1 cut(s) 549
HphI GGTGA 2 cut(s) 292, 409
Hpy188I TCNGA 1 cut(s) 506
Hpy188III TCNNGA 2 cut(s) 111, 202
HpyAV CCTTC 1 cut(s) 550
HpyCH4V TGCA 5 cut(s) 15, 260, 392, 477, 521
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 518
HpyF3I CTNAG 2 cut(s) 447, 503
Hsp92I GRCGYC 1 cut(s) 218
Hsp92II CATG 3 cut(s) 78, 216, 440
HspAI GCGC 1 cut(s) 218
KasI GGCGCC 1 cut(s) 217
Kzo9I GATC 2 cut(s) 87, 208
LmnI GCTCC 1 cut(s) 544
Lsp1109I GCAGC 1 cut(s) 272
LweI GCATC 2 cut(s) 486, 508
MaeIII GTNAC 1 cut(s) 432
MalI GATC 2 cut(s) 89, 210
MboI GATC 2 cut(s) 87, 208
MboII GAAGA 3 cut(s) 177, 536, 577
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 2 cut(s) 384, 400
Mly113I GGCGCC 1 cut(s) 218
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 5 cut(s) 63, 135, 161, 442, 553
Mph1103I ATGCAT 1 cut(s) 479
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 1 cut(s) 549
MspR9I CCNGG 2 cut(s) 281, 550
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 2 cut(s) 159, 518
NarI GGCGCC 1 cut(s) 218
NciI CCSGG 1 cut(s) 550
NcoI CCATGG 1 cut(s) 212
NdeI CATATG 1 cut(s) 473
NdeII GATC 2 cut(s) 87, 208
NlaIII CATG 3 cut(s) 78, 216, 440
NlaIV GGNNCC 4 cut(s) 70, 89, 219, 367
NsiI ATGCAT 1 cut(s) 479
PaqCI CACCTGC 1 cut(s) 20
PctI GAATGC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 122, 147
PkrI GCNGC 1 cut(s) 262
PleI GAGTC 1 cut(s) 224
PluTI GGCGCC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 279
PspFI CCCAGC 1 cut(s) 221
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 4 cut(s) 70, 89, 219, 367
PspPI GGNCC 1 cut(s) 366
PsuI RGATCY 1 cut(s) 87
PvuII CAGCTG 1 cut(s) 253
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 2 cut(s) 87, 208
Sau96I GGNCC 1 cut(s) 366
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 281, 550
SduI GDGCHC 1 cut(s) 502
SfaNI GCATC 2 cut(s) 486, 508
SfoI GGCGCC 1 cut(s) 219
Sse9I AATT 2 cut(s) 384, 400
SsiI CCGC 2 cut(s) 85, 441
SspDI GGCGCC 1 cut(s) 217
StyD4I CCNGG 2 cut(s) 279, 548
StyI CCWWGG 1 cut(s) 212
TaqI TCGA 1 cut(s) 426
TasI AATT 2 cut(s) 384, 400
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 2 cut(s) 122, 147
TseI GCWGC 1 cut(s) 260
TspDTI ATGAA 2 cut(s) 286, 402
XapI RAATTY 1 cut(s) 384
Zsp2I ATGCAT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.