FvH4_5g10160

methyltransferase PMT18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004896
Physical Location & Seq
Reverse (-)
5795975 .. 5801092
5118 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10160.t3

Sequence Viewer

Length: 1461 bp
ATGGCTAGGGAGTATGGAGGTGGATCACCGAAGCACCACCAGCTTGAATCCAAGAAGAAGAGGCTTACTATGATCTTTGGGGTTAGTGCACTTTGCGTAGTATGCTACTGCTTAGGAGCTTGGCAAACCACTTCTGCTCCCGTCAGTCGGTCTGAGCTCTACAACAGGGTTGGTTGCGACGAAGCTTCTAGCCAAGCAAAGATCTCCAATGCCTCATCGGGGCCTGCTGGACTTCACTTGGATTTCGAAAGCCATCACCAAGTGGAGATCAACAAATCTGAGGCAGTCCAGACGTTCCCACCGTGTTCCATGGTATACAGTGAGTACACTCCTTGCCAAGATACAAATAGGGGGAGAAAATTCGACAGGCAAATGTTGAAGTACAGAGAGCGACATTGTCCCACCAAAGAAGAACAACTTCTTTGCTTGATACCTGCTCCACCAAAGTACAAGACTCCTTTTAAGTGGCCTCAGAGCAGGGATTTTGCCTGGTATGACAACATTCCGCATAGGGAGCTTAGCATTGAGAAAGCTGTTCAGAATTGGATTCAAGTTGAGGGTGACCGCTTTAGATTCCCTGGTGGAGGCACCATGTTTCCGAGAGGAGCTGATGCTTACATAGATGATATTAATAAGCTCATTCCTCTTACTAATGGTCACATCAGAACTGCAATTGATACAGGCTGTGGTGTTGCAAGTTGGGGTGCTTACTTGTTGAAGAGGGATATCTTGACAATGTCGTTTGCTCCGAGAGATACGCATGCAGCACAGGTCCAGTTCGCATTGGAGAGGGGAGTTCCTGCTATGATTGGTGTCTTGGGTTCGAAAAGGCTTCCCTACCCGGCCAGGTCTTTTGATATGGCTCACTGTTCCCGCTGCTTGATACCATGGCAGAATTATGATGGTTTGTATCTGATTGAAGTGGACAGAGTTCTAAGGCCGGGTGCTTACTGGATTCTTTCTGGGCCCCCTATTAATTGGAAAAAACACTGGAGAGGATGGGAGAGAACCCAAGAAGACTTGAAACAAGAGCAAGATGCTATCGAGGCTGTTGCAAATAGCTTGTGCTGGAAGAAAGTGATTCAGAAAAATGATCTTGCTATCTGGCAAAAGCCTCTCAATCACGTTGAATGCATCCGAAGCAGGAAGGTCATTAAGACGCCACATATATGCAAATCAGACAATCCAGATATGGCTTGGTACTACTACTCCTCCCAAGGGACAGAAATATGGAAACTTGCATTACACCTCTGCCAGAGCCGGGTAATCCAAATGACATTGCTGGTGGGGCATTGGAGAAGTGGCCTGAGCGTGCATTTGCCGTACCTCCAAGAATCAGCAGTGGTTCAATACCAGGAATCACCCCTGAGAAACTCAAAGAGGATAATGCTCTGTGGAAGGAGAGGGTGGAACATTACAAGAATAAAATACCAATTAGCAAAGGAAGGTATAGGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

487

Amino Acids

55.67

Weight (kDa)

9.21

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 108 - 402 2.2e-134 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 442
AccB1I GGYRCC 1 cut(s) 587
AccI GTMKAC 1 cut(s) 315
AciI CCGC 3 cut(s) 506, 565, 874
AclWI GGATC 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 843
AcsI RAATTY 1 cut(s) 359
AcyI GRCGYC 1 cut(s) 1160
AdeI CACNNNGTG 1 cut(s) 262
AfaI GTAC 5 cut(s) 326, 383, 449, 1202, 1325
AfiI CCNNNNNNNGG 5 cut(s) 147, 219, 584, 1218, 1261
AgsI TTSAA 8 cut(s) 47, 379, 551, 718, 920, 1024, 1130, 1349
AjnI CCWGG 4 cut(s) 488, 577, 845, 1353
AluBI AGCT 9 cut(s) 43, 119, 157, 185, 517, 533, 608, 637, 1062
AluI AGCT 9 cut(s) 43, 119, 157, 185, 517, 533, 608, 637, 1062
Alw21I GWGCWC 2 cut(s) 91, 159
Alw44I GTGCAC 1 cut(s) 87
AlwI GGATC 1 cut(s) 31
AoxI GGCC 6 cut(s) 221, 467, 843, 938, 965, 1303
ApaI GGGCCC 1 cut(s) 969
ApaLI GTGCAC 1 cut(s) 87
ApeKI GCWGC 2 cut(s) 764, 876
ApoI RAATTY 1 cut(s) 359
AseI ATTAAT 2 cut(s) 630, 975
Asp700I GAANNNNTTC 1 cut(s) 417
AspS9I GGNCC 4 cut(s) 221, 772, 965, 966
AsuC2I CCSGG 3 cut(s) 842, 942, 1262
AsuHPI GGTGA 4 cut(s) 18, 248, 572, 1353
AsuII TTCGAA 2 cut(s) 246, 824
AvaII GGWCC 1 cut(s) 772
BaeGI GKGCMC 2 cut(s) 91, 969
BanI GGYRCC 1 cut(s) 587
BanII GRGCYC 2 cut(s) 159, 969
BbsI GAAGAC 1 cut(s) 1023
Bbv12I GWGCWC 2 cut(s) 91, 159
BbvI GCAGC 2 cut(s) 776, 863
BccI CCATC 3 cut(s) 261, 896, 993
BceAI ACGGC 1 cut(s) 1306
BcgI CGANNNNNNTGC 2 cut(s) 1034, 1068
BciT130I CCWGG 4 cut(s) 490, 579, 847, 1355
BcnI CCSGG 3 cut(s) 842, 942, 1262
BfaI CTAG 2 cut(s) 6, 189
BfuAI ACCTGC 1 cut(s) 442
BglII AGATCT 1 cut(s) 201
BisI GCNGC 2 cut(s) 765, 877
BlpI GCTNAGC 1 cut(s) 518
BlsI GCNGC 2 cut(s) 766, 878
Bme1390I CCNGG 7 cut(s) 490, 579, 842, 847, 942, 1262, 1355
Bme18I GGWCC 1 cut(s) 772
BmgT120I GGNCC 4 cut(s) 221, 772, 965, 966
BmiI GGNNCC 4 cut(s) 222, 589, 967, 968
BmrFI CCNGG 7 cut(s) 490, 579, 842, 847, 942, 1262, 1355
BmsI GCATC 3 cut(s) 601, 1027, 1143
BpiI GAAGAC 1 cut(s) 1023
BpmI CTGGAG 1 cut(s) 1012
Bpu10I CCTNAGC 2 cut(s) 112, 1307
Bpu1102I GCTNAGC 1 cut(s) 518
Bpu14I TTCGAA 2 cut(s) 246, 824
BpuMI CCSGG 3 cut(s) 842, 942, 1262
BsaHI GRCGYC 1 cut(s) 1160
BsaJI CCNNGG 4 cut(s) 309, 577, 887, 1216
BsaXI ACNNNNNCTCC 6 cut(s) 121, 151, 1193, 1196, 1223, 1226
Bsc4I CCNNNNNNNGG 5 cut(s) 147, 219, 584, 1218, 1261
Bse1I ACTGG 3 cut(s) 775, 956, 995
Bse3DI GCAATG 1 cut(s) 1277
BseBI CCWGG 4 cut(s) 490, 579, 847, 1355
BseDI CCNNGG 4 cut(s) 309, 577, 887, 1216
BseGI GGATG 2 cut(s) 1004, 1134
BseLI CCNNNNNNNGG 5 cut(s) 147, 219, 584, 1218, 1261
BseMI GCAATG 1 cut(s) 1277
BseMII CTCAG 5 cut(s) 144, 270, 485, 1298, 1358
BseNI ACTGG 3 cut(s) 775, 956, 995
BseRI GAGGAG 2 cut(s) 618, 1201
BseSI GKGCMC 2 cut(s) 91, 969
BseXI GCAGC 2 cut(s) 776, 863
BshFI GGCC 6 cut(s) 223, 469, 845, 940, 967, 1305
BshNI GGYRCC 1 cut(s) 587
BsiHKAI GWGCWC 2 cut(s) 91, 159
BsiSI CCGG 3 cut(s) 842, 941, 1261
BslFI GGGAC 2 cut(s) 384, 1234
BslI CCNNNNNNNGG 5 cut(s) 147, 219, 584, 1218, 1261
BsmFI GGGAC 2 cut(s) 384, 1234
BsmI GAATGC 1 cut(s) 1136
BsnI GGCC 6 cut(s) 223, 469, 845, 940, 967, 1305
Bsp119I TTCGAA 2 cut(s) 246, 824
Bsp120I GGGCCC 1 cut(s) 965
Bsp1286I GDGCHC 3 cut(s) 91, 159, 969
Bsp143I GATC 5 cut(s) 23, 72, 201, 267, 1093
Bsp1720I GCTNAGC 1 cut(s) 518
Bsp19I CCATGG 2 cut(s) 309, 887
BspACI CCGC 3 cut(s) 506, 565, 874
BspANI GGCC 6 cut(s) 223, 469, 845, 940, 967, 1305
BspCNI CTCAG 5 cut(s) 145, 271, 484, 1299, 1359
BspLI GGNNCC 4 cut(s) 222, 589, 967, 968
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 1 cut(s) 31
BspT104I TTCGAA 2 cut(s) 246, 824
BspT107I GGYRCC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 1277
BsrI ACTGG 3 cut(s) 775, 956, 995
BssECI CCNNGG 4 cut(s) 309, 577, 887, 1216
BssMI GATC 5 cut(s) 23, 72, 201, 267, 1093
BssNAI GTATAC 1 cut(s) 316
BssNI GRCGYC 1 cut(s) 1160
BssT1I CCWWGG 3 cut(s) 309, 887, 1216
Bst1107I GTATAC 1 cut(s) 316
Bst2UI CCWGG 4 cut(s) 490, 579, 847, 1355
Bst4CI ACNGT 3 cut(s) 303, 320, 869
Bst6I CTCTTC 2 cut(s) 53, 713
BstACI GRCGYC 1 cut(s) 1160
BstBI TTCGAA 2 cut(s) 246, 824
BstC8I GCNNGC 3 cut(s) 225, 762, 1313
BstDEI CTNAG 8 cut(s) 112, 153, 279, 471, 518, 935, 1307, 1367
BstDSI CCRYGG 2 cut(s) 309, 887
BstEII GGTNACC 1 cut(s) 560
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 2 cut(s) 1004, 1134
BstKTI GATC 5 cut(s) 26, 75, 204, 270, 1096
BstMBI GATC 5 cut(s) 23, 72, 201, 267, 1093
BstMWI GCNNNNNNNGC 6 cut(s) 40, 102, 514, 1046, 1140, 1288
BstNI CCWGG 4 cut(s) 490, 579, 847, 1355
BstNSI RCATGY 1 cut(s) 764
BstPI GGTNACC 1 cut(s) 560
BstSCI CCNGG 7 cut(s) 488, 577, 840, 845, 940, 1260, 1353
BstSLI GKGCMC 2 cut(s) 91, 969
BstV1I GCAGC 2 cut(s) 776, 863
BstV2I GAAGAC 1 cut(s) 1023
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
BstZ17I GTATAC 1 cut(s) 316
BsuRI GGCC 6 cut(s) 223, 469, 845, 940, 967, 1305
BtgI CCRYGG 2 cut(s) 309, 887
BtsCI GGATG 2 cut(s) 1004, 1134
BtsI GCAGTG 1 cut(s) 1347
BtsIMutI CAGTG 4 cut(s) 325, 865, 988, 1347
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 3 cut(s) 225, 762, 1313
Cfr13I GGNCC 4 cut(s) 221, 772, 965, 966
CseI GACGC 1 cut(s) 1168
Csp6I GTAC 5 cut(s) 325, 382, 448, 1201, 1324
CviAII CATG 4 cut(s) 310, 592, 761, 888
CviQI GTAC 5 cut(s) 325, 382, 448, 1201, 1324
DdeI CTNAG 8 cut(s) 112, 153, 279, 471, 518, 935, 1307, 1367
DpnI GATC 5 cut(s) 25, 74, 203, 269, 1095
DpnII GATC 5 cut(s) 23, 72, 201, 267, 1093
DraIII CACNNNGTG 1 cut(s) 262
EaeI YGGCCR 1 cut(s) 843
Eam1104I CTCTTC 2 cut(s) 53, 713
EarI CTCTTC 2 cut(s) 53, 713
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 3 cut(s) 309, 887, 1216
Eco24I GRGCYC 2 cut(s) 159, 969
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 772
Eco53kI GAGCTC 1 cut(s) 157
Eco91I GGTNACC 1 cut(s) 560
EcoICRI GAGCTC 1 cut(s) 157
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoO109I RGGNCCY 2 cut(s) 221, 966
EcoO65I GGTNACC 1 cut(s) 560
EcoRII CCWGG 4 cut(s) 488, 577, 845, 1353
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 3 cut(s) 309, 887, 1216
EcoT22I ATGCAT 1 cut(s) 1136
EcoT38I GRGCYC 2 cut(s) 159, 969
ErhI CCWWGG 3 cut(s) 309, 887, 1216
FaeI CATG 4 cut(s) 313, 595, 764, 891
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 2 cut(s) 384, 1234
FatI CATG 4 cut(s) 309, 591, 760, 887
FauI CCCGC 1 cut(s) 881
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 2 cut(s) 765, 877
FokI GGATG 2 cut(s) 1011, 1121
FriOI GRGCYC 2 cut(s) 159, 969
Fsp4HI GCNGC 2 cut(s) 765, 877
FspBI CTAG 2 cut(s) 6, 189
GluI GCNGC 2 cut(s) 765, 877
GsuI CTGGAG 1 cut(s) 1012
HaeIII GGCC 6 cut(s) 223, 469, 845, 940, 967, 1305
HapII CCGG 3 cut(s) 842, 941, 1261
HgaI GACGC 1 cut(s) 1168
Hin1I GRCGYC 1 cut(s) 1160
Hin1II CATG 4 cut(s) 313, 595, 764, 891
HindIII AAGCTT 1 cut(s) 183
HinfI GANTC 8 cut(s) 47, 454, 547, 573, 955, 1081, 1334, 1358
HpaII CCGG 3 cut(s) 842, 941, 1261
HphI GGTGA 4 cut(s) 18, 248, 572, 1353
Hpy166II GTNNAC 4 cut(s) 89, 316, 327, 925
Hpy188III TCNNGA 3 cut(s) 289, 730, 1187
Hpy8I GTNNAC 4 cut(s) 89, 316, 327, 925
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 3 cut(s) 1141, 1392, 1439
HpyCH4III ACNGT 3 cut(s) 303, 320, 869
HpyCH4IV ACGT 2 cut(s) 293, 1125
HpyCH4V TGCA 9 cut(s) 89, 671, 695, 764, 1055, 1134, 1173, 1241, 1315
HpyF10VI GCNNNNNNNGC 6 cut(s) 40, 102, 514, 1046, 1140, 1288
HpyF3I CTNAG 8 cut(s) 112, 153, 279, 471, 518, 935, 1307, 1367
HpySE526I ACGT 2 cut(s) 293, 1125
Hsp92I GRCGYC 1 cut(s) 1160
Hsp92II CATG 4 cut(s) 313, 595, 764, 891
Kzo9I GATC 5 cut(s) 23, 72, 201, 267, 1093
LmnI GCTCC 6 cut(s) 116, 142, 442, 514, 605, 751
Lsp1109I GCAGC 2 cut(s) 776, 863
LweI GCATC 3 cut(s) 601, 1027, 1143
MaeI CTAG 2 cut(s) 6, 189
MaeII ACGT 2 cut(s) 293, 1125
MaeIII GTNAC 2 cut(s) 560, 656
MalI GATC 5 cut(s) 25, 74, 203, 269, 1095
MboI GATC 5 cut(s) 23, 72, 201, 267, 1093
MboII GAAGA 6 cut(s) 67, 70, 422, 730, 1028, 1084
MfeI CAATTG 1 cut(s) 672
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 3 cut(s) 91, 159, 969
MluCI AATT 6 cut(s) 359, 541, 672, 895, 976, 1433
MlyI GAGTC 1 cut(s) 448
Mph1103I ATGCAT 1 cut(s) 1136
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 4 cut(s) 462, 630, 975, 1155
MslI CAYNNNNRTG 1 cut(s) 1168
MspA1I CMGCKG 1 cut(s) 876
MspI CCGG 3 cut(s) 842, 941, 1261
MspR9I CCNGG 7 cut(s) 490, 579, 842, 847, 942, 1262, 1355
MunI CAATTG 1 cut(s) 672
Mva1269I GAATGC 1 cut(s) 1136
MvaI CCWGG 4 cut(s) 490, 579, 847, 1355
MwoI GCNNNNNNNGC 6 cut(s) 40, 102, 514, 1046, 1140, 1288
NciI CCSGG 3 cut(s) 842, 942, 1262
NcoI CCATGG 2 cut(s) 309, 887
NdeII GATC 5 cut(s) 23, 72, 201, 267, 1093
NlaIII CATG 4 cut(s) 313, 595, 764, 891
NlaIV GGNNCC 4 cut(s) 222, 589, 967, 968
NmuCI GTSAC 2 cut(s) 560, 656
NsiI ATGCAT 1 cut(s) 1136
NspI RCATGY 1 cut(s) 764
NspV TTCGAA 2 cut(s) 246, 824
PaeI GCATGC 1 cut(s) 764
PcsI WCGNNNNNNNCGW 2 cut(s) 299, 746
PctI GAATGC 1 cut(s) 1136
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 7 cut(s) 47, 547, 573, 955, 1081, 1334, 1358
PflFI GACNNNGTC 2 cut(s) 396, 736
PkrI GCNGC 2 cut(s) 766, 878
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PshBI ATTAAT 2 cut(s) 630, 975
Psp124BI GAGCTC 1 cut(s) 159
Psp6I CCWGG 4 cut(s) 488, 577, 845, 1353
PspEI GGTNACC 1 cut(s) 560
PspGI CCWGG 4 cut(s) 488, 577, 845, 1353
PspN4I GGNNCC 4 cut(s) 222, 589, 967, 968
PspOMI GGGCCC 1 cut(s) 965
PspPI GGNCC 4 cut(s) 221, 772, 965, 966
PsuI RGATCY 1 cut(s) 201
PsyI GACNNNGTC 2 cut(s) 396, 736
RsaI GTAC 5 cut(s) 326, 383, 449, 1202, 1325
RsaNI GTAC 5 cut(s) 325, 382, 448, 1201, 1324
RseI CAYNNNNRTG 1 cut(s) 1168
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 4 cut(s) 462, 630, 975, 1155
SatI GCNGC 2 cut(s) 765, 877
Sau3AI GATC 5 cut(s) 23, 72, 201, 267, 1093
Sau96I GGNCC 4 cut(s) 221, 772, 965, 966
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 7 cut(s) 490, 579, 842, 847, 942, 1262, 1355
SduI GDGCHC 3 cut(s) 91, 159, 969
SfaNI GCATC 3 cut(s) 601, 1027, 1143
SfuI TTCGAA 2 cut(s) 246, 824
SinI GGWCC 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 1168
SphI GCATGC 1 cut(s) 764
Sse9I AATT 6 cut(s) 359, 541, 672, 895, 976, 1433
SsiI CCGC 3 cut(s) 506, 565, 874
SspMI CTAG 2 cut(s) 6, 189
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 7 cut(s) 488, 577, 840, 845, 940, 1260, 1353
StyI CCWWGG 3 cut(s) 309, 887, 1216
TaaI ACNGT 3 cut(s) 303, 320, 869
TaiI ACGT 2 cut(s) 296, 1128
TaqI TCGA 4 cut(s) 246, 363, 824, 1044
TaqII GACCGA 1 cut(s) 138
TasI AATT 6 cut(s) 359, 541, 672, 895, 976, 1433
TatI WGTACW 3 cut(s) 324, 381, 447
TfiI GAWTC 7 cut(s) 47, 547, 573, 955, 1081, 1334, 1358
Tru1I TTAA 4 cut(s) 462, 630, 975, 1155
Tru9I TTAA 4 cut(s) 462, 630, 975, 1155
TscAI CASTG 4 cut(s) 325, 872, 995, 1347
TseFI GTSAC 2 cut(s) 560, 656
TseI GCWGC 2 cut(s) 764, 876
Tsp45I GTSAC 2 cut(s) 560, 656
TspRI CASTG 4 cut(s) 325, 872, 995, 1347
Tth111I GACNNNGTC 2 cut(s) 396, 736
VneI GTGCAC 1 cut(s) 87
VpaK11BI GGWCC 1 cut(s) 772
VspI ATTAAT 2 cut(s) 630, 975
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 1 cut(s) 359
XceI RCATGY 1 cut(s) 764
XcmI CCANNNNNNNNNTGG 1 cut(s) 1194
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 2 cut(s) 6, 189
Zsp2I ATGCAT 1 cut(s) 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.