pycom06g11770

methyltransferase PMT18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004896
Physical Location & Seq
Forward (+)
16970760 .. 16973173
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g11770.3

Sequence Viewer

Length: 1899 bp
ATGGCCAGGGATTATAGTGGTGGATCGCCAAAGCATCACCACATAGAATCCAAGAAGAAGAGGCTCACTTGGATATTCGGGGTTAGTGCGCTCTGCATAGTATGTTACATGTTAGGAGCTTGGCAAACCACTTCTGCCCCTGTCAATCAATCTGAGCTCTACCAAAGGGTTGGTTGTGATGAAACGCCATCTCAGTCCGGGAACAAAACTTCCTCATCCGTGTCATCATCATCATCATCGCTTCACTTGGACTTCGAAAGCCATCATCAAGTGGACATCAACAAAACCGAGGCTGTCCAGAAGTTCCCACCGTGTGACATGTCCTACAGCGAGTACACTCCATGCCAGGATGCAACAAGGGGGAGGAAATTCGACAGGAGCATGTTGAAATACAGAGAGAGGCATTGCCCTACCAAAGAAGAACAACTTCTTTGCCTTATTCCTGCTCCACCAAAGTACAAGACCCCTTTCAAGTGGCCTCAGAGTCGTGATTTTGCTTGGTATGACAACATTCCCCATAGAGAACTCAGCATTGAGAAAGCTGTCCAAAATTGGATTCAAGTCGAGGGTGAAAGGTTTAGATTCCCCGGGGGAGGTACCGGGTTTCCAAGGGGAGCTGATGCATACATAGATGAAATGAACGAGCTCATTCCTCTCACTAAGGGCAACATCAGAACTGCAATTGATACCGGCTGTGGTGTAGCCAGTTGGGGTGCTTACCTGTTGAAGAGGGACATCTTGACAATGTCTTTTGCGCCGAGAGATACGCATGCAGCGCAGGTCCAGTTTGCATTGGAGCGTGGAGTTCCTGCTATGATTGGTGTCATGGCTTCGAAGAGGCTTCCCTACCCTGCCAGGGCTTTCGATATGGCTCACTGTTCCCGCTGCTTGATCCCTTGGACGAACTATGATGGTATGTATCTAATTGAAGTGGACAGAGTTCTAAGGCCTGGTGGTTATTGGATTCTTTCCGGGCCGCCTATTCACTGGAAAAAACACTGGAGAGGATGGGAGAGAACTCAACAGGATTTGAAACAAGAGCAAGATTCTATTGAGTCTCTTGCAAAGCGCCTGTGCTGGAAGAAGGTGATCGAGAAGATCGATCTTGCAATCTGGCAAAAACCTATCAATCATGTCGAATGCATTAAGAGCAGGAAGGTGTATAAAACACCACATATATGCAAATCAGACAATCCAGATATGGCTTGGGAAAGAGAAATGGAGAGTTGCATTACTCCTCTACCGGAGACAAGCAATCCCAATGATGTTGCTGGTGGGGCGTTGGAGAAGTGGCCTCAGCGTGCATTTGCTATCCCGCCTAGAATCAGCAGTGGTTCAATACCAGGAATCACTGCTGATAAACTCCGCGAGGATAATCAACTGTGGAAGGAAAGGGTGGACCATTACAAGCGGATCGTACCAATTTCCAAAGGAAGGTATAGGAATGTGATGGACATGAATGCTTACCTCGGTGGATTTGCTGCAGCGTTGTCAAAATATCCGGTGTGGGTTATGAACACAGTCCCTGCCAACTCAAACCAGGACACTCTTGGAGTGATTTACGAACGGGGTTTCATTGGACATTACCAGGATTGGTGCGAGGCATTGTCCACATATCCCAGAACATACGATTTCATCCATGCTGGGGGAGTCTTCAGCATATACCAGGACAGGTGTGACATAACACTCATTTTACTGGAGATGGATAGGATTCTGAGGCCGGAAGGAACGGTTGTGTTTAGGGATACTGTAGAGATCTTGGTGAAAATAAAGGCGATTACCGACGGGATGAGATGGAAGAGCCAGATTCTGGATCATGAAAGTGGACCTTTTAATCCAGAGAAAATTCTTCTTGCAGTGAAAACTTACTGGACTGGTGAAGAAAAAAAAGGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

633

Amino Acids

72.27

Weight (kDa)

8.67

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 769
Acc65I GGTACC 1 cut(s) 596
AccB1I GGYRCC 1 cut(s) 596
AccB7I CCANNNNNTGG 1 cut(s) 1810
AccII CGCG 1 cut(s) 1368
AciI CCGC 5 cut(s) 883, 977, 1316, 1366, 1411
AclWI GGATC 4 cut(s) 31, 886, 1421, 1821
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 368, 1845
AcuI CTGAAG 1 cut(s) 1639
AdeI CACNNNGTG 1 cut(s) 314
AfaI GTAC 4 cut(s) 335, 458, 598, 1419
AfiI CCNNNNNNNGG 5 cut(s) 593, 856, 1434, 1645, 1810
AflIII ACRYGT 2 cut(s) 108, 318
AgsI TTSAA 7 cut(s) 388, 472, 560, 727, 929, 1033, 1338
AjnI CCWGG 8 cut(s) 5, 345, 854, 949, 1342, 1539, 1587, 1665
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AloI GAACNNNNNNTCC 2 cut(s) 194, 226
AluBI AGCT 6 cut(s) 119, 157, 542, 617, 646, 1896
AluI AGCT 6 cut(s) 119, 157, 542, 617, 646, 1896
Alw21I GWGCWC 2 cut(s) 159, 648
Alw26I GTCTC 2 cut(s) 1062, 1241
AlwI GGATC 4 cut(s) 31, 886, 1421, 1821
AlwNI CAGNNNCTG 2 cut(s) 1526, 1810
Ama87I CYCGRG 1 cut(s) 587
AoxI GGCC 6 cut(s) 3, 476, 947, 974, 1292, 1718
ApeKI GCWGC 4 cut(s) 773, 885, 1481, 1484
ApoI RAATTY 2 cut(s) 368, 1845
Asp700I GAANNNNTTC 1 cut(s) 426
Asp718I GGTACC 1 cut(s) 596
AspLEI GCGC 4 cut(s) 91, 757, 778, 1071
AspS9I GGNCC 4 cut(s) 781, 974, 1399, 1826
AsuC2I CCSGG 5 cut(s) 199, 588, 589, 601, 973
AsuHPI GGTGA 5 cut(s) 29, 581, 1099, 1774, 1889
AsuII TTCGAA 2 cut(s) 255, 833
AvaI CYCGRG 1 cut(s) 587
AvaII GGWCC 3 cut(s) 781, 1399, 1826
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 596
BanII GRGCYC 2 cut(s) 159, 648
BbsI GAAGAC 1 cut(s) 1645
Bbv12I GWGCWC 2 cut(s) 159, 648
BbvCI CCTCAGC 1 cut(s) 1296
BbvI GCAGC 4 cut(s) 785, 872, 1468, 1496
BccI CCATC 7 cut(s) 196, 270, 905, 1002, 1444, 1696, 1788
BciT130I CCWGG 8 cut(s) 7, 347, 856, 951, 1344, 1541, 1589, 1667
BciVI GTATCC 1 cut(s) 1738
BcnI CCSGG 5 cut(s) 199, 588, 589, 601, 973
BcoDI GTCTC 2 cut(s) 1062, 1241
BfaI CTAG 2 cut(s) 1320, 1897
BfmI CTRYAG 3 cut(s) 325, 1482, 1749
BfoI RGCGCY 1 cut(s) 1072
BfuAI ACCTGC 1 cut(s) 769
BfuI GTATCC 1 cut(s) 1738
BglII AGATCT 1 cut(s) 1755
BisI GCNGC 5 cut(s) 774, 886, 977, 1482, 1485
BlsI GCNGC 5 cut(s) 775, 887, 978, 1483, 1486
Bme18I GGWCC 3 cut(s) 781, 1399, 1826
BmeT110I CYCGRG 1 cut(s) 587
BmgT120I GGNCC 4 cut(s) 781, 974, 1399, 1826
BmiI GGNNCC 1 cut(s) 598
BmsI GCATC 3 cut(s) 43, 340, 610
BpiI GAAGAC 1 cut(s) 1645
BpmI CTGGAG 2 cut(s) 1021, 1718
Bpu10I CCTNAGC 1 cut(s) 1296
Bpu14I TTCGAA 2 cut(s) 255, 833
BpuMI CCSGG 5 cut(s) 199, 588, 589, 601, 973
Bsa29I ATCGAT 1 cut(s) 1101
BsaJI CCNNGG 9 cut(s) 6, 288, 586, 587, 588, 608, 855, 896, 1468
BsaWI WCCGGW 2 cut(s) 1243, 1501
Bsc4I CCNNNNNNNGG 5 cut(s) 593, 856, 1434, 1645, 1810
Bse118I RCCGGY 1 cut(s) 689
Bse1I ACTGG 7 cut(s) 705, 784, 992, 1004, 1701, 1874, 1879
Bse3DI GCAATG 1 cut(s) 403
BseBI CCWGG 8 cut(s) 7, 347, 856, 951, 1344, 1541, 1589, 1667
BseCI ATCGAT 1 cut(s) 1101
BseDI CCNNGG 9 cut(s) 6, 288, 586, 587, 588, 608, 855, 896, 1468
BseGI GGATG 5 cut(s) 215, 355, 1013, 1635, 1794
BseLI CCNNNNNNNGG 5 cut(s) 593, 856, 1434, 1645, 1810
BseMI GCAATG 1 cut(s) 403
BseMII CTCAG 6 cut(s) 144, 206, 494, 541, 1310, 1706
BseNI ACTGG 7 cut(s) 705, 784, 992, 1004, 1701, 1874, 1879
BseRI GAGGAG 1 cut(s) 1227
BseXI GCAGC 4 cut(s) 785, 872, 1468, 1496
BseYI CCCAGC 1 cut(s) 1643
Bsh1236I CGCG 1 cut(s) 1368
BshFI GGCC 6 cut(s) 5, 478, 949, 976, 1294, 1720
BshNI GGYRCC 1 cut(s) 596
BshVI ATCGAT 1 cut(s) 1101
BsiHKAI GWGCWC 2 cut(s) 159, 648
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 8 cut(s) 198, 588, 600, 690, 972, 1244, 1502, 1721
BslFI GGGAC 2 cut(s) 746, 1508
BslI CCNNNNNNNGG 5 cut(s) 593, 856, 1434, 1645, 1810
BsmAI GTCTC 2 cut(s) 1062, 1241
BsmFI GGGAC 2 cut(s) 746, 1508
BsmI GAATGC 2 cut(s) 1145, 1465
BsnI GGCC 6 cut(s) 5, 478, 949, 976, 1294, 1720
BsoBI CYCGRG 1 cut(s) 587
Bsp119I TTCGAA 2 cut(s) 255, 833
Bsp1286I GDGCHC 2 cut(s) 159, 648
Bsp143I GATC 8 cut(s) 23, 891, 1089, 1098, 1102, 1413, 1755, 1813
BspACI CCGC 5 cut(s) 883, 977, 1316, 1366, 1411
BspANI GGCC 6 cut(s) 5, 478, 949, 976, 1294, 1720
BspCNI CTCAG 6 cut(s) 145, 205, 493, 540, 1309, 1707
BspDI ATCGAT 1 cut(s) 1101
BspFNI CGCG 1 cut(s) 1368
BspHI TCATGA 1 cut(s) 1816
BspLI GGNNCC 1 cut(s) 598
BspMAI CTGCAG 1 cut(s) 1486
BspMI ACCTGC 1 cut(s) 769
BspPI GGATC 4 cut(s) 31, 886, 1421, 1821
BspQI GCTCTTC 1 cut(s) 1793
BspT104I TTCGAA 2 cut(s) 255, 833
BspT107I GGYRCC 1 cut(s) 596
BsrDI GCAATG 1 cut(s) 403
BsrFI RCCGGY 1 cut(s) 689
BsrI ACTGG 7 cut(s) 705, 784, 992, 1004, 1701, 1874, 1879
BssAI RCCGGY 1 cut(s) 689
BssECI CCNNGG 9 cut(s) 6, 288, 586, 587, 588, 608, 855, 896, 1468
BssMI GATC 8 cut(s) 23, 891, 1089, 1098, 1102, 1413, 1755, 1813
BssT1I CCWWGG 2 cut(s) 608, 896
Bst2UI CCWGG 8 cut(s) 7, 347, 856, 951, 1344, 1541, 1589, 1667
Bst4CI ACNGT 6 cut(s) 312, 878, 1383, 1522, 1732, 1750
Bst6I CTCTTC 4 cut(s) 53, 722, 830, 1793
BstBI TTCGAA 2 cut(s) 255, 833
BstC8I GCNNGC 2 cut(s) 771, 1302
BstDEI CTNAG 8 cut(s) 153, 192, 480, 527, 660, 944, 1296, 1715
BstF5I GGATG 5 cut(s) 215, 355, 1013, 1635, 1794
BstFNI CGCG 1 cut(s) 1368
BstH2I RGCGCY 1 cut(s) 1072
BstHHI GCGC 4 cut(s) 91, 757, 778, 1071
BstKTI GATC 8 cut(s) 26, 894, 1092, 1101, 1105, 1416, 1758, 1816
BstMAI GTCTC 2 cut(s) 1062, 1241
BstMBI GATC 8 cut(s) 23, 891, 1089, 1098, 1102, 1413, 1755, 1813
BstMWI GCNNNNNNNGC 3 cut(s) 775, 1149, 1277
BstNI CCWGG 8 cut(s) 7, 347, 856, 951, 1344, 1541, 1589, 1667
BstNSI RCATGY 4 cut(s) 112, 322, 385, 773
BstSFI CTRYAG 3 cut(s) 325, 1482, 1749
BstUI CGCG 1 cut(s) 1368
BstV1I GCAGC 4 cut(s) 785, 872, 1468, 1496
BstV2I GAAGAC 1 cut(s) 1645
BstX2I RGATCY 1 cut(s) 1755
BstXI CCANNNNNNTGG 1 cut(s) 170
BstYI RGATCY 1 cut(s) 1755
Bsu15I ATCGAT 1 cut(s) 1101
BsuI GTATCC 1 cut(s) 1738
BsuRI GGCC 6 cut(s) 5, 478, 949, 976, 1294, 1720
BsuTUI ATCGAT 1 cut(s) 1101
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 5 cut(s) 215, 355, 1013, 1635, 1794
BtsI GCAGTG 3 cut(s) 1336, 1350, 1863
BtsIMutI CAGTG 6 cut(s) 874, 985, 997, 1336, 1350, 1863
BveI ACCTGC 1 cut(s) 769
Cac8I GCNNGC 2 cut(s) 771, 1302
CaiI CAGNNNCTG 2 cut(s) 1526, 1810
CciI TCATGA 1 cut(s) 1816
CfoI GCGC 4 cut(s) 91, 757, 778, 1071
Cfr10I RCCGGY 1 cut(s) 689
Cfr13I GGNCC 4 cut(s) 781, 974, 1399, 1826
Cfr9I CCCGGG 1 cut(s) 587
ClaI ATCGAT 1 cut(s) 1101
Csp6I GTAC 4 cut(s) 334, 457, 597, 1418
CviQI GTAC 4 cut(s) 334, 457, 597, 1418
DdeI CTNAG 8 cut(s) 153, 192, 480, 527, 660, 944, 1296, 1715
DpnI GATC 8 cut(s) 25, 893, 1091, 1100, 1104, 1415, 1757, 1815
DpnII GATC 8 cut(s) 23, 891, 1089, 1098, 1102, 1413, 1755, 1813
DraIII CACNNNGTG 1 cut(s) 314
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 53, 722, 830, 1793
EarI CTCTTC 4 cut(s) 53, 722, 830, 1793
Ecl136II GAGCTC 2 cut(s) 157, 646
Eco130I CCWWGG 2 cut(s) 608, 896
Eco147I AGGCCT 1 cut(s) 949
Eco24I GRGCYC 2 cut(s) 159, 648
Eco47I GGWCC 3 cut(s) 781, 1399, 1826
Eco53kI GAGCTC 2 cut(s) 157, 646
Eco57I CTGAAG 1 cut(s) 1639
Eco88I CYCGRG 1 cut(s) 587
EcoICRI GAGCTC 2 cut(s) 157, 646
EcoRII CCWGG 8 cut(s) 5, 345, 854, 949, 1342, 1539, 1587, 1665
EcoT14I CCWWGG 2 cut(s) 608, 896
EcoT22I ATGCAT 2 cut(s) 625, 1145
EcoT38I GRGCYC 2 cut(s) 159, 648
ErhI CCWWGG 2 cut(s) 608, 896
FalI AAGNNNNNCTT 4 cut(s) 411, 443, 1813, 1845
FaqI GGGAC 2 cut(s) 746, 1508
FauI CCCGC 2 cut(s) 890, 1323
Fnu4HI GCNGC 5 cut(s) 774, 886, 977, 1482, 1485
FokI GGATG 5 cut(s) 202, 362, 1020, 1622, 1801
FriOI GRGCYC 2 cut(s) 159, 648
Fsp4HI GCNGC 5 cut(s) 774, 886, 977, 1482, 1485
FspBI CTAG 2 cut(s) 1320, 1897
GlaI GCGC 4 cut(s) 90, 756, 777, 1070
GluI GCNGC 5 cut(s) 774, 886, 977, 1482, 1485
GsaI CCCAGC 1 cut(s) 1647
GsuI CTGGAG 2 cut(s) 1021, 1718
HaeII RGCGCY 1 cut(s) 1072
HaeIII GGCC 6 cut(s) 5, 478, 949, 976, 1294, 1720
HapII CCGG 8 cut(s) 198, 588, 600, 690, 972, 1244, 1502, 1721
HhaI GCGC 4 cut(s) 91, 757, 778, 1071
Hin6I GCGC 4 cut(s) 89, 755, 776, 1069
HinP1I GCGC 4 cut(s) 89, 755, 776, 1069
HpaII CCGG 8 cut(s) 198, 588, 600, 690, 972, 1244, 1502, 1721
HphI GGTGA 5 cut(s) 29, 581, 1099, 1774, 1889
Hpy166II GTNNAC 6 cut(s) 274, 336, 934, 1399, 1611, 1826
Hpy188I TCNGA 5 cut(s) 154, 483, 674, 1189, 1716
Hpy188III TCNNGA 8 cut(s) 298, 488, 739, 1093, 1196, 1811, 1817, 1838
Hpy8I GTNNAC 6 cut(s) 274, 336, 934, 1399, 1611, 1826
Hpy99I CGWCG 1 cut(s) 1787
HpyAV CCTTC 5 cut(s) 1078, 1150, 1381, 1428, 1718
HpyCH4III ACNGT 6 cut(s) 312, 878, 1383, 1522, 1732, 1750
HpyF10VI GCNNNNNNNGC 3 cut(s) 775, 1149, 1277
HpyF3I CTNAG 8 cut(s) 153, 192, 480, 527, 660, 944, 1296, 1715
HspAI GCGC 4 cut(s) 89, 755, 776, 1069
KpnI GGTACC 1 cut(s) 600
Kzo9I GATC 8 cut(s) 23, 891, 1089, 1098, 1102, 1413, 1755, 1813
LguI GCTCTTC 1 cut(s) 1793
LmnI GCTCC 5 cut(s) 116, 378, 451, 614, 796
Lsp1109I GCAGC 4 cut(s) 785, 872, 1468, 1496
LweI GCATC 3 cut(s) 43, 340, 610
MaeI CTAG 2 cut(s) 1320, 1897
MaeIII GTNAC 3 cut(s) 104, 314, 1676
MalI GATC 8 cut(s) 25, 893, 1091, 1100, 1104, 1415, 1757, 1815
MboI GATC 8 cut(s) 23, 891, 1089, 1098, 1102, 1413, 1755, 1813
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 1 cut(s) 1755
MhlI GDGCHC 2 cut(s) 159, 648
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 368, 550, 681, 924, 1422, 1845
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 493, 1064, 1659
MmeI TCCRAC 1 cut(s) 1263
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 625, 1145
MroXI GAANNNNTTC 1 cut(s) 426
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 1146, 1833
MslI CAYNNNNRTG 2 cut(s) 1177, 1821
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 885
MspI CCGG 8 cut(s) 198, 588, 600, 690, 972, 1244, 1502, 1721
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 2 cut(s) 1145, 1465
MvaI CCWGG 8 cut(s) 7, 347, 856, 951, 1344, 1541, 1589, 1667
MvnI CGCG 1 cut(s) 1368
MwoI GCNNNNNNNGC 3 cut(s) 775, 1149, 1277
NciI CCSGG 5 cut(s) 199, 588, 589, 601, 973
NdeII GATC 8 cut(s) 23, 891, 1089, 1098, 1102, 1413, 1755, 1813
NlaIV GGNNCC 1 cut(s) 598
NmeAIII GCCGAG 1 cut(s) 783
NmuCI GTSAC 2 cut(s) 314, 1676
NsiI ATGCAT 2 cut(s) 625, 1145
NspI RCATGY 4 cut(s) 112, 322, 385, 773
NspV TTCGAA 2 cut(s) 255, 833
PaeI GCATGC 1 cut(s) 773
PagI TCATGA 1 cut(s) 1816
PceI AGGCCT 1 cut(s) 949
PciI ACATGT 2 cut(s) 108, 318
PciSI GCTCTTC 1 cut(s) 1793
PcsI WCGNNNNNNNCGW 1 cut(s) 1098
PctI GAATGC 2 cut(s) 1145, 1465
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 9 cut(s) 47, 556, 582, 964, 1046, 1323, 1347, 1711, 1807
PflFI GACNNNGTC 1 cut(s) 745
PflMI CCANNNNNTGG 1 cut(s) 1810
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 5 cut(s) 775, 887, 978, 1483, 1486
PleI GAGTC 3 cut(s) 492, 1063, 1658
PpsI GAGTC 3 cut(s) 492, 1063, 1658
PscI ACATGT 2 cut(s) 108, 318
Psp124BI GAGCTC 2 cut(s) 159, 648
Psp6I CCWGG 8 cut(s) 5, 345, 854, 949, 1342, 1539, 1587, 1665
PspFI CCCAGC 1 cut(s) 1643
PspGI CCWGG 8 cut(s) 5, 345, 854, 949, 1342, 1539, 1587, 1665
PspN4I GGNNCC 1 cut(s) 598
PspPI GGNCC 4 cut(s) 781, 974, 1399, 1826
PstI CTGCAG 1 cut(s) 1486
PstNI CAGNNNCTG 2 cut(s) 1526, 1810
PsuI RGATCY 1 cut(s) 1755
PsyI GACNNNGTC 1 cut(s) 745
RsaI GTAC 4 cut(s) 335, 458, 598, 1419
RsaNI GTAC 4 cut(s) 334, 457, 597, 1418
RseI CAYNNNNRTG 2 cut(s) 1177, 1821
SacI GAGCTC 2 cut(s) 159, 648
SapI GCTCTTC 1 cut(s) 1793
SaqAI TTAA 2 cut(s) 1146, 1833
SatI GCNGC 5 cut(s) 774, 886, 977, 1482, 1485
Sau3AI GATC 8 cut(s) 23, 891, 1089, 1098, 1102, 1413, 1755, 1813
Sau96I GGNCC 4 cut(s) 781, 974, 1399, 1826
SchI GAGTC 3 cut(s) 493, 1064, 1659
SduI GDGCHC 2 cut(s) 159, 648
SfaNI GCATC 3 cut(s) 43, 340, 610
SfcI CTRYAG 3 cut(s) 325, 1482, 1749
SfuI TTCGAA 2 cut(s) 255, 833
SinI GGWCC 3 cut(s) 781, 1399, 1826
SmaI CCCGGG 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 1177, 1821
SphI GCATGC 1 cut(s) 773
Sse9I AATT 6 cut(s) 368, 550, 681, 924, 1422, 1845
SseBI AGGCCT 1 cut(s) 949
SsiI CCGC 5 cut(s) 883, 977, 1316, 1366, 1411
SspMI CTAG 2 cut(s) 1320, 1897
SstI GAGCTC 2 cut(s) 159, 648
StuI AGGCCT 1 cut(s) 949
StyI CCWWGG 2 cut(s) 608, 896
TaaI ACNGT 6 cut(s) 312, 878, 1383, 1522, 1732, 1750
TaqI TCGA 8 cut(s) 255, 372, 564, 833, 864, 1092, 1101, 1137
TasI AATT 6 cut(s) 368, 550, 681, 924, 1422, 1845
TatI WGTACW 2 cut(s) 333, 456
TauI GCSGC 1 cut(s) 979
TfiI GAWTC 9 cut(s) 47, 556, 582, 964, 1046, 1323, 1347, 1711, 1807
Tru1I TTAA 2 cut(s) 1146, 1833
Tru9I TTAA 2 cut(s) 1146, 1833
TscAI CASTG 6 cut(s) 881, 992, 1004, 1336, 1357, 1863
TseFI GTSAC 2 cut(s) 314, 1676
TseI GCWGC 4 cut(s) 773, 885, 1481, 1484
Tsp45I GTSAC 2 cut(s) 314, 1676
TspDTI ATGAA 8 cut(s) 195, 648, 653, 1472, 1529, 1564, 1624, 1833
TspGWI ACGGA 1 cut(s) 208
TspMI CCCGGG 1 cut(s) 587
TspRI CASTG 6 cut(s) 881, 992, 1004, 1336, 1357, 1863
Tth111I GACNNNGTC 1 cut(s) 745
Van91I CCANNNNNTGG 1 cut(s) 1810
VpaK11BI GGWCC 3 cut(s) 781, 1399, 1826
XapI RAATTY 2 cut(s) 368, 1845
XceI RCATGY 4 cut(s) 112, 322, 385, 773
XcmI CCANNNNNNNNNTGG 2 cut(s) 1203, 1547
XmaI CCCGGG 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 426
XspI CTAG 2 cut(s) 1320, 1897
Zsp2I ATGCAT 2 cut(s) 625, 1145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.