MD06G1127600.v1.1

methyltransferase PMT18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004896
Physical Location & Seq
Forward (+)
26963973 .. 26967663
3691 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1127600.v1.1.491

Sequence Viewer

Length: 1791 bp
ATGTTAGGAGCTTGGCAAACCACTTCTGCCCCTGTCAATCAATCTGAGCTCTACCAAAGGGTTGGTTGTGATGAAACGCCATCTCAATCCGGGAACAAAACTTCCTCATCCGTGTCATCATCATCATCATCGCTTCACTTGGACTTCGAAAGCCATCATCAAGTGGACATCAACAAAACTGAGGCTGTCCAGAAGTTCCCACCGTGTGACATGTCCTACAGCGAGTACACTCCATGCCAGGATGCAACAAGGGGGAGGAAATTCGACAGGAGCATGTTAAAATACAGAGAGAGGCATTGCCCTACCAAAGAAGAACAACTTCTTTGCCTTATTCCTGCTCCACCAAAGTACAAGACCCCTTTCAAGTGGCCTCAGAGTCGTGATTTTGCTTGGTATGACAACATTCCCCACAGAGAACTCAGCATTGAGAAAGCTGTCCAAAATTGGATTCAAGTCGAGGGTGACAGGTTTAGATTCCCCGGGGGAGGTACCGGGTTTCCAAGGGGAGCTGATGCATACATAGATGAAATGAACGCGCTCATTCCTCTCACTAAGGGCAACATCAGAACTGCAATTGATACCGGCTGTGGTGTAGCCAGTTGGGGTGCTTACCTGTTGAAGAGGGACATCTTGACAATGTCTTTTGCGCCAAGAGATACGCATGCAGCGCAGGTCCAGTTTGCATTGGAGCGTGGAGTTCCTGCTATGATTGGTGTCATGGCTTCGAAGAGGCTTCCCTACCCTGCAAGGGCCTTCGATATGGCTCACTGTTCCCGCTGCTTGATCCCTTGGACGAACTATGATGGTATGTATCTAATTGAAGTGGACAGAGTTCTAAGGCCTGGTGGTTATTGGATTCTTTCCGGGCCGCCTATTCACTGGAAAAAACACTGGAGAGGATGGGAGAGAACTCAACAGGATTTGAAACAAGAGCAAGATTCTATTGAGTCTCTTGCAAAGCGCCTGTGCTGGAAGAAGGTGATCGAGAAGAACGATCTAGCAATCTGGCAAAAACCTATCAATCACGTCGAATGCATTAAGAGCAGGAAGGTGTATAAAACACCACATATATGCAAATCAGACAATCCAGATATGGCTTGGGAAAGAGAAATGGAGAGTTGCATTTCTCCTCTACCGGAGACGAGCAATCCCAATGATGTTGCTGGTGGGGCGTTGGAGAAGTGGCCTCAGCGTGCATTTGCTATCCCGCCTAGAATCAGCAGTGGTTCAATACCAGGAATCACTGCTGATAAACTCCGTGAGGATAATCAACTGTGGAAGGAAAGGGTGGACCATTACAAGCGGATCGTACCAATTTCCAAGGGAAGGTATAGGAATGTGATGGACATGAATGCTTACCTCGGTGGATTTGCTGCAGCATTGTCAAAATATCCGGTGTGGGTTATGAACACAGTCCCTGCCAACTCAAACCAGGACACTCTTGGAGTGATTTACGAACGGGGTTTCATTGGACATTACCAGGATTGGTGCGAGGCATTGTCCACATATCCCAGAACATACGATTTCATCCACGCTGGGGGAGTCTTCAGCATATACCAGGACAGGTGTGACATAACACTCATTTTACTGGAGATGGATAGGATTCTGAGGCCGGAAGGAACTGTTGTGTTTAGGGATACTGTAGAGATCCTGGTGAAAGTAAAGGCGATTACCGACGGAATGAGATGGAAGAGCCAGATTCTGGATCATGAAAGTGGACCTTTTAATCCAGAGAAAATTCTTCTTGCAGTGAAAACTTACTGGACTGGTGAAGAAAAAAAAGGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

597

Amino Acids

68.07

Weight (kDa)

8.48

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 75 - 585 1.5e-226 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 661
Acc65I GGTACC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 488
AccB7I CCANNNNNTGG 1 cut(s) 1702
AccII CGCG 1 cut(s) 536
AciI CCGC 4 cut(s) 775, 869, 1208, 1303
AclWI GGATC 4 cut(s) 778, 1313, 1642, 1713
AcsI RAATTY 2 cut(s) 260, 1737
AcuI CTGAAG 1 cut(s) 1531
AdeI CACNNNGTG 1 cut(s) 206
AfaI GTAC 4 cut(s) 227, 350, 490, 1311
AfiI CCNNNNNNNGG 5 cut(s) 485, 748, 1326, 1537, 1702
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 6 cut(s) 364, 452, 619, 821, 925, 1230
AjiI CACGTC 1 cut(s) 1027
AjnI CCWGG 7 cut(s) 237, 841, 1234, 1431, 1479, 1557, 1650
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AloI GAACNNNNNNTCC 2 cut(s) 86, 118
AluBI AGCT 5 cut(s) 11, 49, 434, 509, 1788
AluI AGCT 5 cut(s) 11, 49, 434, 509, 1788
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 2 cut(s) 954, 1133
AlwI GGATC 4 cut(s) 778, 1313, 1642, 1713
AlwNI CAGNNNCTG 2 cut(s) 1418, 1702
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 6 cut(s) 368, 750, 839, 866, 1184, 1610
ApeKI GCWGC 4 cut(s) 665, 777, 1373, 1376
ApoI RAATTY 2 cut(s) 260, 1737
Asp700I GAANNNNTTC 1 cut(s) 318
Asp718I GGTACC 1 cut(s) 488
AspLEI GCGC 4 cut(s) 538, 649, 670, 963
AspS9I GGNCC 5 cut(s) 673, 750, 866, 1291, 1718
AsuC2I CCSGG 5 cut(s) 91, 480, 481, 493, 865
AsuHPI GGTGA 4 cut(s) 473, 991, 1666, 1781
AsuII TTCGAA 2 cut(s) 147, 725
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 3 cut(s) 673, 1291, 1718
BanI GGYRCC 1 cut(s) 488
BanII GRGCYC 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 1537
Bbv12I GWGCWC 1 cut(s) 51
BbvCI CCTCAGC 1 cut(s) 1188
BbvI GCAGC 4 cut(s) 677, 764, 1360, 1388
BccI CCATC 7 cut(s) 88, 162, 797, 894, 1336, 1588, 1680
BciT130I CCWGG 7 cut(s) 239, 843, 1236, 1433, 1481, 1559, 1652
BciVI GTATCC 1 cut(s) 1630
BcnI CCSGG 5 cut(s) 91, 480, 481, 493, 865
BcoDI GTCTC 2 cut(s) 954, 1133
BfaI CTAG 3 cut(s) 998, 1212, 1789
BfmI CTRYAG 3 cut(s) 217, 1374, 1641
BfoI RGCGCY 1 cut(s) 964
BfuAI ACCTGC 1 cut(s) 661
BfuI GTATCC 1 cut(s) 1630
BisI GCNGC 5 cut(s) 666, 778, 869, 1374, 1377
BlsI GCNGC 5 cut(s) 667, 779, 870, 1375, 1378
Bme18I GGWCC 3 cut(s) 673, 1291, 1718
BmeT110I CYCGRG 1 cut(s) 479
BmgBI CACGTC 1 cut(s) 1027
BmgT120I GGNCC 5 cut(s) 673, 750, 866, 1291, 1718
BmiI GGNNCC 1 cut(s) 490
BmsI GCATC 2 cut(s) 232, 502
BpiI GAAGAC 1 cut(s) 1537
BpmI CTGGAG 2 cut(s) 913, 1610
Bpu10I CCTNAGC 1 cut(s) 1188
Bpu14I TTCGAA 2 cut(s) 147, 725
BpuMI CCSGG 5 cut(s) 91, 480, 481, 493, 865
BsaJI CCNNGG 7 cut(s) 478, 479, 480, 500, 788, 1320, 1360
BsaWI WCCGGW 2 cut(s) 1135, 1393
Bsc4I CCNNNNNNNGG 5 cut(s) 485, 748, 1326, 1537, 1702
Bse118I RCCGGY 1 cut(s) 581
Bse1I ACTGG 7 cut(s) 597, 676, 884, 896, 1593, 1766, 1771
Bse3DI GCAATG 1 cut(s) 295
BseBI CCWGG 7 cut(s) 239, 843, 1236, 1433, 1481, 1559, 1652
BseDI CCNNGG 7 cut(s) 478, 479, 480, 500, 788, 1320, 1360
BseGI GGATG 4 cut(s) 107, 247, 905, 1527
BseLI CCNNNNNNNGG 5 cut(s) 485, 748, 1326, 1537, 1702
BseMI GCAATG 1 cut(s) 295
BseMII CTCAG 6 cut(s) 36, 171, 386, 433, 1202, 1598
BseNI ACTGG 7 cut(s) 597, 676, 884, 896, 1593, 1766, 1771
BseRI GAGGAG 1 cut(s) 1119
BseXI GCAGC 4 cut(s) 677, 764, 1360, 1388
BseYI CCCAGC 1 cut(s) 1535
Bsh1236I CGCG 1 cut(s) 536
BshFI GGCC 6 cut(s) 370, 752, 841, 868, 1186, 1612
BshNI GGYRCC 1 cut(s) 488
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 8 cut(s) 90, 480, 492, 582, 864, 1136, 1394, 1613
BslFI GGGAC 2 cut(s) 638, 1400
BslI CCNNNNNNNGG 5 cut(s) 485, 748, 1326, 1537, 1702
BsmAI GTCTC 2 cut(s) 954, 1133
BsmBI CGTCTC 1 cut(s) 1133
BsmFI GGGAC 2 cut(s) 638, 1400
BsmI GAATGC 2 cut(s) 1037, 1357
BsnI GGCC 6 cut(s) 370, 752, 841, 868, 1186, 1612
BsoBI CYCGRG 1 cut(s) 479
Bsp119I TTCGAA 2 cut(s) 147, 725
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 6 cut(s) 783, 981, 994, 1305, 1647, 1705
BspACI CCGC 4 cut(s) 775, 869, 1208, 1303
BspANI GGCC 6 cut(s) 370, 752, 841, 868, 1186, 1612
BspCNI CTCAG 6 cut(s) 37, 172, 385, 432, 1201, 1599
BspFNI CGCG 1 cut(s) 536
BspHI TCATGA 1 cut(s) 1708
BspLI GGNNCC 1 cut(s) 490
BspMAI CTGCAG 1 cut(s) 1378
BspMI ACCTGC 1 cut(s) 661
BspPI GGATC 4 cut(s) 778, 1313, 1642, 1713
BspQI GCTCTTC 1 cut(s) 1685
BspT104I TTCGAA 2 cut(s) 147, 725
BspT107I GGYRCC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 295
BsrFI RCCGGY 1 cut(s) 581
BsrI ACTGG 7 cut(s) 597, 676, 884, 896, 1593, 1766, 1771
BssAI RCCGGY 1 cut(s) 581
BssECI CCNNGG 7 cut(s) 478, 479, 480, 500, 788, 1320, 1360
BssMI GATC 6 cut(s) 783, 981, 994, 1305, 1647, 1705
BssT1I CCWWGG 3 cut(s) 500, 788, 1320
Bst2UI CCWGG 7 cut(s) 239, 843, 1236, 1433, 1481, 1559, 1652
Bst4CI ACNGT 6 cut(s) 204, 770, 1275, 1414, 1624, 1642
Bst6I CTCTTC 3 cut(s) 614, 722, 1685
BstBI TTCGAA 2 cut(s) 147, 725
BstC8I GCNNGC 2 cut(s) 663, 1194
BstDEI CTNAG 8 cut(s) 45, 180, 372, 419, 552, 836, 1188, 1607
BstF5I GGATG 4 cut(s) 107, 247, 905, 1527
BstFNI CGCG 1 cut(s) 536
BstH2I RGCGCY 1 cut(s) 964
BstHHI GCGC 4 cut(s) 538, 649, 670, 963
BstKTI GATC 6 cut(s) 786, 984, 997, 1308, 1650, 1708
BstMAI GTCTC 2 cut(s) 954, 1133
BstMBI GATC 6 cut(s) 783, 981, 994, 1305, 1647, 1705
BstMWI GCNNNNNNNGC 3 cut(s) 667, 1041, 1169
BstNI CCWGG 7 cut(s) 239, 843, 1236, 1433, 1481, 1559, 1652
BstNSI RCATGY 3 cut(s) 214, 277, 665
BstSFI CTRYAG 3 cut(s) 217, 1374, 1641
BstUI CGCG 1 cut(s) 536
BstV1I GCAGC 4 cut(s) 677, 764, 1360, 1388
BstV2I GAAGAC 1 cut(s) 1537
BstX2I RGATCY 1 cut(s) 1647
BstXI CCANNNNNNTGG 1 cut(s) 62
BstYI RGATCY 1 cut(s) 1647
BsuI GTATCC 1 cut(s) 1630
BsuRI GGCC 6 cut(s) 370, 752, 841, 868, 1186, 1612
BtgZI GCGATG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 1027
BtsCI GGATG 4 cut(s) 107, 247, 905, 1527
BtsI GCAGTG 3 cut(s) 1228, 1242, 1755
BtsIMutI CAGTG 6 cut(s) 766, 877, 889, 1228, 1242, 1755
BveI ACCTGC 1 cut(s) 661
Cac8I GCNNGC 2 cut(s) 663, 1194
CaiI CAGNNNCTG 2 cut(s) 1418, 1702
CciI TCATGA 1 cut(s) 1708
CfoI GCGC 4 cut(s) 538, 649, 670, 963
Cfr10I RCCGGY 1 cut(s) 581
Cfr13I GGNCC 5 cut(s) 673, 750, 866, 1291, 1718
Cfr9I CCCGGG 1 cut(s) 479
Csp6I GTAC 4 cut(s) 226, 349, 489, 1310
CviAII CATG 7 cut(s) 211, 234, 274, 662, 718, 1348, 1709
CviQI GTAC 4 cut(s) 226, 349, 489, 1310
DdeI CTNAG 8 cut(s) 45, 180, 372, 419, 552, 836, 1188, 1607
DpnI GATC 6 cut(s) 785, 983, 996, 1307, 1649, 1707
DpnII GATC 6 cut(s) 783, 981, 994, 1305, 1647, 1705
DraIII CACNNNGTG 1 cut(s) 206
Eam1104I CTCTTC 3 cut(s) 614, 722, 1685
EarI CTCTTC 3 cut(s) 614, 722, 1685
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 3 cut(s) 500, 788, 1320
Eco147I AGGCCT 1 cut(s) 841
Eco24I GRGCYC 1 cut(s) 51
Eco47I GGWCC 3 cut(s) 673, 1291, 1718
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 1531
Eco88I CYCGRG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 750
EcoRII CCWGG 7 cut(s) 237, 841, 1234, 1431, 1479, 1557, 1650
EcoT14I CCWWGG 3 cut(s) 500, 788, 1320
EcoT22I ATGCAT 2 cut(s) 517, 1037
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 3 cut(s) 500, 788, 1320
Esp3I CGTCTC 1 cut(s) 1133
FaeI CATG 7 cut(s) 214, 237, 277, 665, 721, 1351, 1712
FalI AAGNNNNNCTT 4 cut(s) 303, 335, 1705, 1737
FaqI GGGAC 2 cut(s) 638, 1400
FatI CATG 7 cut(s) 210, 233, 273, 661, 717, 1347, 1708
FauI CCCGC 2 cut(s) 782, 1215
Fnu4HI GCNGC 5 cut(s) 666, 778, 869, 1374, 1377
FokI GGATG 4 cut(s) 94, 254, 912, 1514
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 5 cut(s) 666, 778, 869, 1374, 1377
FspBI CTAG 3 cut(s) 998, 1212, 1789
GlaI GCGC 4 cut(s) 537, 648, 669, 962
GluI GCNGC 5 cut(s) 666, 778, 869, 1374, 1377
GsaI CCCAGC 1 cut(s) 1539
GsuI CTGGAG 2 cut(s) 913, 1610
HaeII RGCGCY 1 cut(s) 964
HaeIII GGCC 6 cut(s) 370, 752, 841, 868, 1186, 1612
HapII CCGG 8 cut(s) 90, 480, 492, 582, 864, 1136, 1394, 1613
HhaI GCGC 4 cut(s) 538, 649, 670, 963
Hin1II CATG 7 cut(s) 214, 237, 277, 665, 721, 1351, 1712
Hin6I GCGC 4 cut(s) 536, 647, 668, 961
HinP1I GCGC 4 cut(s) 536, 647, 668, 961
HpaII CCGG 8 cut(s) 90, 480, 492, 582, 864, 1136, 1394, 1613
HphI GGTGA 4 cut(s) 473, 991, 1666, 1781
Hpy166II GTNNAC 6 cut(s) 166, 228, 826, 1291, 1503, 1718
Hpy188I TCNGA 5 cut(s) 46, 375, 566, 1081, 1608
Hpy188III TCNNGA 8 cut(s) 190, 380, 631, 985, 1088, 1703, 1709, 1730
Hpy8I GTNNAC 6 cut(s) 166, 228, 826, 1291, 1503, 1718
Hpy99I CGWCG 2 cut(s) 1031, 1679
HpyAV CCTTC 6 cut(s) 763, 970, 1042, 1273, 1320, 1610
HpyCH4III ACNGT 6 cut(s) 204, 770, 1275, 1414, 1624, 1642
HpyCH4IV ACGT 1 cut(s) 1026
HpyF10VI GCNNNNNNNGC 3 cut(s) 667, 1041, 1169
HpyF3I CTNAG 8 cut(s) 45, 180, 372, 419, 552, 836, 1188, 1607
HpySE526I ACGT 1 cut(s) 1026
Hsp92II CATG 7 cut(s) 214, 237, 277, 665, 721, 1351, 1712
HspAI GCGC 4 cut(s) 536, 647, 668, 961
KpnI GGTACC 1 cut(s) 492
Kzo9I GATC 6 cut(s) 783, 981, 994, 1305, 1647, 1705
LguI GCTCTTC 1 cut(s) 1685
LmnI GCTCC 5 cut(s) 8, 270, 343, 506, 688
Lsp1109I GCAGC 4 cut(s) 677, 764, 1360, 1388
LweI GCATC 2 cut(s) 232, 502
MaeI CTAG 3 cut(s) 998, 1212, 1789
MaeII ACGT 1 cut(s) 1026
MaeIII GTNAC 3 cut(s) 206, 461, 1568
MalI GATC 6 cut(s) 785, 983, 996, 1307, 1649, 1707
MboI GATC 6 cut(s) 783, 981, 994, 1305, 1647, 1705
MboII GAAGA 9 cut(s) 323, 631, 739, 985, 1000, 1537, 1702, 1733, 1784
MfeI CAATTG 1 cut(s) 573
MflI RGATCY 1 cut(s) 1647
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 6 cut(s) 260, 442, 573, 816, 1314, 1737
MlyI GAGTC 3 cut(s) 385, 956, 1551
MmeI TCCRAC 1 cut(s) 1155
Mph1103I ATGCAT 2 cut(s) 517, 1037
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 3 cut(s) 278, 1038, 1725
MslI CAYNNNNRTG 2 cut(s) 1069, 1713
MspA1I CMGCKG 1 cut(s) 777
MspI CCGG 8 cut(s) 90, 480, 492, 582, 864, 1136, 1394, 1613
MunI CAATTG 1 cut(s) 573
Mva1269I GAATGC 2 cut(s) 1037, 1357
MvaI CCWGG 7 cut(s) 239, 843, 1236, 1433, 1481, 1559, 1652
MvnI CGCG 1 cut(s) 536
MwoI GCNNNNNNNGC 3 cut(s) 667, 1041, 1169
NciI CCSGG 5 cut(s) 91, 480, 481, 493, 865
NdeII GATC 6 cut(s) 783, 981, 994, 1305, 1647, 1705
NlaIII CATG 7 cut(s) 214, 237, 277, 665, 721, 1351, 1712
NlaIV GGNNCC 1 cut(s) 490
NmuCI GTSAC 3 cut(s) 206, 461, 1568
NsiI ATGCAT 2 cut(s) 517, 1037
NspI RCATGY 3 cut(s) 214, 277, 665
NspV TTCGAA 2 cut(s) 147, 725
PaeI GCATGC 1 cut(s) 665
PagI TCATGA 1 cut(s) 1708
PceI AGGCCT 1 cut(s) 841
PciI ACATGT 1 cut(s) 210
PciSI GCTCTTC 1 cut(s) 1685
PcsI WCGNNNNNNNCGW 1 cut(s) 990
PctI GAATGC 2 cut(s) 1037, 1357
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 8 cut(s) 448, 474, 856, 938, 1215, 1239, 1603, 1699
PflFI GACNNNGTC 1 cut(s) 637
PflMI CCANNNNNTGG 1 cut(s) 1702
PfoI TCCNGGA 1 cut(s) 89
PkrI GCNGC 5 cut(s) 667, 779, 870, 1375, 1378
PleI GAGTC 3 cut(s) 384, 955, 1550
PpsI GAGTC 3 cut(s) 384, 955, 1550
PscI ACATGT 1 cut(s) 210
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 7 cut(s) 237, 841, 1234, 1431, 1479, 1557, 1650
PspFI CCCAGC 1 cut(s) 1535
PspGI CCWGG 7 cut(s) 237, 841, 1234, 1431, 1479, 1557, 1650
PspN4I GGNNCC 1 cut(s) 490
PspPI GGNCC 5 cut(s) 673, 750, 866, 1291, 1718
PstI CTGCAG 1 cut(s) 1378
PstNI CAGNNNCTG 2 cut(s) 1418, 1702
PsuI RGATCY 1 cut(s) 1647
PsyI GACNNNGTC 1 cut(s) 637
RsaI GTAC 4 cut(s) 227, 350, 490, 1311
RsaNI GTAC 4 cut(s) 226, 349, 489, 1310
RseI CAYNNNNRTG 2 cut(s) 1069, 1713
SacI GAGCTC 1 cut(s) 51
SapI GCTCTTC 1 cut(s) 1685
SaqAI TTAA 3 cut(s) 278, 1038, 1725
SatI GCNGC 5 cut(s) 666, 778, 869, 1374, 1377
Sau3AI GATC 6 cut(s) 783, 981, 994, 1305, 1647, 1705
Sau96I GGNCC 5 cut(s) 673, 750, 866, 1291, 1718
SchI GAGTC 3 cut(s) 385, 956, 1551
SduI GDGCHC 1 cut(s) 51
SfaNI GCATC 2 cut(s) 232, 502
SfcI CTRYAG 3 cut(s) 217, 1374, 1641
SfuI TTCGAA 2 cut(s) 147, 725
SinI GGWCC 3 cut(s) 673, 1291, 1718
SmaI CCCGGG 1 cut(s) 481
SmiMI CAYNNNNRTG 2 cut(s) 1069, 1713
SphI GCATGC 1 cut(s) 665
Sse9I AATT 6 cut(s) 260, 442, 573, 816, 1314, 1737
SseBI AGGCCT 1 cut(s) 841
SsiI CCGC 4 cut(s) 775, 869, 1208, 1303
SspMI CTAG 3 cut(s) 998, 1212, 1789
SstI GAGCTC 1 cut(s) 51
StuI AGGCCT 1 cut(s) 841
StyI CCWWGG 3 cut(s) 500, 788, 1320
TaaI ACNGT 6 cut(s) 204, 770, 1275, 1414, 1624, 1642
TaiI ACGT 1 cut(s) 1029
TaqI TCGA 7 cut(s) 147, 264, 456, 725, 756, 984, 1029
TasI AATT 6 cut(s) 260, 442, 573, 816, 1314, 1737
TatI WGTACW 2 cut(s) 225, 348
TauI GCSGC 1 cut(s) 871
TfiI GAWTC 8 cut(s) 448, 474, 856, 938, 1215, 1239, 1603, 1699
Tru1I TTAA 3 cut(s) 278, 1038, 1725
Tru9I TTAA 3 cut(s) 278, 1038, 1725
TscAI CASTG 6 cut(s) 773, 884, 896, 1228, 1249, 1755
TseFI GTSAC 3 cut(s) 206, 461, 1568
TseI GCWGC 4 cut(s) 665, 777, 1373, 1376
Tsp45I GTSAC 3 cut(s) 206, 461, 1568
TspDTI ATGAA 8 cut(s) 87, 540, 545, 1364, 1421, 1456, 1516, 1725
TspGWI ACGGA 3 cut(s) 100, 1247, 1692
TspMI CCCGGG 1 cut(s) 479
TspRI CASTG 6 cut(s) 773, 884, 896, 1228, 1249, 1755
Tth111I GACNNNGTC 1 cut(s) 637
Van91I CCANNNNNTGG 1 cut(s) 1702
VpaK11BI GGWCC 3 cut(s) 673, 1291, 1718
XapI RAATTY 2 cut(s) 260, 1737
XceI RCATGY 3 cut(s) 214, 277, 665
XcmI CCANNNNNNNNNTGG 2 cut(s) 1095, 1439
XmaI CCCGGG 1 cut(s) 479
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 3 cut(s) 998, 1212, 1789
Zsp2I ATGCAT 2 cut(s) 517, 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.