MD14G1143200.v1.1

methyltransferase PMT18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004896
Physical Location & Seq
Forward (+)
23596225 .. 23600334
4110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1143200.v1.1.491

Sequence Viewer

Length: 1926 bp
ATGCTTTTATCACATCAGTTACAAAAGAATAAAATGGCCAGGGATATTAGTGGTGGATCGCAAAAGCACCACCACGTAGAATCCAAGAAGAAGAGGCTCACTTGGATCTTCGGGATTTTTGCGCTCTGCATAGCATGTTACATGTTAGGAGCTTGGCAAACCACTTCTGCCCCTGTCAATCGATCTGAGCTCTACCAAAGGGTTGGTTGTGATGAAACGTCATCTCAATCCGGGAACAAAACCTCCTCACCCGTGTCATCATCTCCGCTTCGCTTGGACTTCGAAAGCCATCATCAAGTGGACGTCAACAAAACCGAGGCCGTTCAAAAGTTCCCACCGTGTGACATGTCCTACAGCGAGTACACTCCATGCCAGGATCCAAGAAGGGGGAGGAAATTTGACAGGAAAATGTTGAAACACAGAGAGAGGCACTGCCCTACCAAAGAAGAACAACTTCTTTGCCTTATTCCTGCTCCGCCAAAGTATAAGACTCCTTTCAAGTGGCCTCAGAGTCGTGATTTTGCTTGGTATGACAACATTCCCCATAGAGAACTCAGCATTGAGAAAGCTGTCCAGAATTGGATTCAAGTCGAGGGTGACAGGTTTAGATTCCCCGGGGGAGGTACCATGTTTCCAAGGGGAGCTGATGCGTACATAGATGACATTAACGGACTCATTCCTCTCACTAAGGGCAACATCAGAACTGCAATTGATACCGGCTGTGGTGTAGCCAGTTGGGGTGCTTACCTGTTGAAGAGGAACATCGTGACAATGTCTTTTGCACCGAGAGATTCGCATGCAGCGCAAGTTCAGTTTGCATTGGAGCGTGGAGTTCCCGCTATGATTGGTGTCATGGCTTCTAAGAGGCTTCCCTACCCTGCAAGGGCTTTCGATATGGCTCACTGTTCCCGCTGCTTGATCCCTTGGATGAACTATGATGGTATGTATCTCATTGAAGTGGACAGAGTTCTAAGGCCTGGTGGTTATTGGGTTCTCTCCGGGCCGCCTATTAACTGGAAAAAACACTGGAGGGGATGGGAGAGAACTGAAGAGGATTTGAAGCAAGAGCAAGATTCTATTGAGTCTGTTGCAAAGCGCCTGTGCTGGAAGAAGGTGATCGAGAAGAATGATCTTGCAATCTGGCAAAAACCTATCAATCACGTCGAATGCATTAAGAGCAGGAAGGTGTATAAAACACCACATATATGCAAATCAGACAATCCAGATATGGCTTGGGAAAGAGACATGGAGAGTTGCATTACTCCTCTACCGGAGACAAGCGATCCTAATGACGTTGCTGGTGGGGCGTTAGAGAAGTGGCCTGAACGTGCATTTGCTGTCCCTCCTAGAATCAGCAGTGGTTCAATACCAGGAATCACAGCTGAGAAACTCCGTGAGGATAATCAACTGTGGAAGGAAAGGGTGGAACATTACAAGAGGATTGTACCAATTTCCAAAGGAAGATATCGGAATGTGATGGACATGAATGCTTACCTCGGTGGATTTGCTGCAGCATTGTCAAAATATCCAGTGTGGGTTATGAACACCGTCCCTGCCAACTCAAACCAGGACACCCTTGGTGTAATTTACGAACGTGGTTTTATTGGAACGTACCAGGACTGGTGTGAGGCTTTCTCCACGTACCCTAGAACATACGATCTCATCCACGCTGGTGGGGTCTTCAGCATATATCAGGACAGGTGTGACATAACACTCATTTTGCTGGAGATGGATAGGATTCTGAGGCCGGAAGGAACAGTTGTGTTTAGGGATACAGTAGAGATCCTGGTGAAGATAAAGGCGATTACCGACGGAATGAGGTGGAAGAGCCAGATTATGGATCACGAGAGTGGACCTTTTAACCCAGAGAAAATTCTTCTTGCAGTGAAAACTTACTGGACTGGTGAAGAAAAAAAGGAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

642

Amino Acids

73.53

Weight (kDa)

8.84

Isoelectric Point (pI)

50.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 120 - 630 8.3e-229 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 306
Acc65I GGTACC 1 cut(s) 623
AccB1I GGYRCC 1 cut(s) 623
AccB7I CCANNNNNTGG 1 cut(s) 1837
AciI CCGC 5 cut(s) 266, 476, 837, 910, 1004
AclWI GGATC 8 cut(s) 64, 113, 371, 384, 913, 1277, 1777, 1848
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 2 cut(s) 395, 1872
AcuI CTGAAG 2 cut(s) 1068, 1666
AcyI GRCGYC 1 cut(s) 303
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 6 cut(s) 362, 625, 653, 1446, 1613, 1643
AfiI CCNNNNNNNGG 4 cut(s) 386, 620, 883, 1837
AflIII ACRYGT 2 cut(s) 141, 345
AgsI TTSAA 8 cut(s) 326, 415, 499, 587, 754, 956, 1060, 1365
AjiI CACGTC 1 cut(s) 1162
AjnI CCWGG 7 cut(s) 38, 372, 976, 1369, 1566, 1614, 1785
AleI CACNNNNGTG 2 cut(s) 1671, 1848
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 6 cut(s) 152, 190, 569, 644, 1382, 1923
AluI AGCT 6 cut(s) 152, 190, 569, 644, 1382, 1923
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 2 cut(s) 1236, 1268
AlwI GGATC 8 cut(s) 64, 113, 371, 384, 913, 1277, 1777, 1848
Ama87I CYCGRG 1 cut(s) 614
AoxI GGCC 7 cut(s) 36, 318, 503, 974, 1001, 1319, 1745
ApeKI GCWGC 4 cut(s) 800, 912, 1508, 1511
ApoI RAATTY 2 cut(s) 395, 1872
Asp700I GAANNNNTTC 1 cut(s) 453
Asp718I GGTACC 1 cut(s) 623
AspLEI GCGC 3 cut(s) 124, 805, 1098
AspS9I GGNCC 2 cut(s) 1001, 1853
AsuC2I CCSGG 4 cut(s) 232, 615, 616, 1000
AsuHPI GGTGA 5 cut(s) 240, 608, 1126, 1801, 1916
AsuII TTCGAA 1 cut(s) 282
AvaI CYCGRG 1 cut(s) 614
AvaII GGWCC 1 cut(s) 1853
BalI TGGCCA 1 cut(s) 38
BamHI GGATCC 1 cut(s) 376
BanI GGYRCC 1 cut(s) 623
BanII GRGCYC 1 cut(s) 192
BauI CACGAG 1 cut(s) 1844
BbsI GAAGAC 1 cut(s) 1672
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 4 cut(s) 812, 899, 1495, 1523
BccI CCATC 5 cut(s) 297, 932, 1029, 1471, 1723
BceAI ACGGC 1 cut(s) 305
BciT130I CCWGG 7 cut(s) 40, 374, 978, 1371, 1568, 1616, 1787
BciVI GTATCC 1 cut(s) 1765
BcnI CCSGG 4 cut(s) 232, 615, 616, 1000
BcoDI GTCTC 2 cut(s) 1236, 1268
BfaI CTAG 3 cut(s) 1347, 1647, 1924
BfmI CTRYAG 2 cut(s) 352, 1509
BfoI RGCGCY 1 cut(s) 1099
BfuI GTATCC 1 cut(s) 1765
BisI GCNGC 5 cut(s) 801, 913, 1004, 1509, 1512
BlsI GCNGC 5 cut(s) 802, 914, 1005, 1510, 1513
Bme18I GGWCC 1 cut(s) 1853
BmeT110I CYCGRG 1 cut(s) 614
BmgBI CACGTC 1 cut(s) 1162
BmgT120I GGNCC 2 cut(s) 1001, 1853
BmiI GGNNCC 2 cut(s) 378, 625
BmsI GCATC 1 cut(s) 637
BpiI GAAGAC 1 cut(s) 1672
BplI GAGNNNNNCTC 2 cut(s) 1619, 1651
BpmI CTGGAG 2 cut(s) 1048, 1745
Bpu14I TTCGAA 1 cut(s) 282
BpuMI CCSGG 4 cut(s) 232, 615, 616, 1000
Bsa29I ATCGAT 1 cut(s) 181
BsaAI YACGTR 2 cut(s) 76, 1641
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 9 cut(s) 39, 315, 613, 614, 615, 635, 923, 1495, 1576
BsaWI WCCGGW 1 cut(s) 1270
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 4 cut(s) 386, 620, 883, 1837
Bse118I RCCGGY 1 cut(s) 716
Bse1I ACTGG 7 cut(s) 732, 1019, 1031, 1529, 1625, 1901, 1906
BseBI CCWGG 7 cut(s) 40, 374, 978, 1371, 1568, 1616, 1787
BseCI ATCGAT 1 cut(s) 181
BseDI CCNNGG 9 cut(s) 39, 315, 613, 614, 615, 635, 923, 1495, 1576
BseGI GGATG 3 cut(s) 933, 1040, 1662
BseLI CCNNNNNNNGG 4 cut(s) 386, 620, 883, 1837
BseMII CTCAG 5 cut(s) 177, 521, 568, 1374, 1733
BseNI ACTGG 7 cut(s) 732, 1019, 1031, 1529, 1625, 1901, 1906
BseRI GAGGAG 2 cut(s) 235, 1254
BseXI GCAGC 4 cut(s) 812, 899, 1495, 1523
BshFI GGCC 7 cut(s) 38, 320, 505, 976, 1003, 1321, 1747
BshNI GGYRCC 1 cut(s) 623
BshVI ATCGAT 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 614
BsiSI CCGG 6 cut(s) 231, 615, 717, 999, 1271, 1748
BslFI GGGAC 2 cut(s) 1325, 1535
BslI CCNNNNNNNGG 4 cut(s) 386, 620, 883, 1837
BsmAI GTCTC 2 cut(s) 1236, 1268
BsmFI GGGAC 2 cut(s) 1325, 1535
BsmI GAATGC 2 cut(s) 1172, 1492
BsnI GGCC 7 cut(s) 38, 320, 505, 976, 1003, 1321, 1747
BsoBI CYCGRG 1 cut(s) 614
Bsp119I TTCGAA 1 cut(s) 282
Bsp1286I GDGCHC 1 cut(s) 192
BspACI CCGC 5 cut(s) 266, 476, 837, 910, 1004
BspANI GGCC 7 cut(s) 38, 320, 505, 976, 1003, 1321, 1747
BspCNI CTCAG 5 cut(s) 178, 520, 567, 1375, 1734
BspDI ATCGAT 1 cut(s) 181
BspLI GGNNCC 2 cut(s) 378, 625
BspMAI CTGCAG 1 cut(s) 1513
BspPI GGATC 8 cut(s) 64, 113, 371, 384, 913, 1277, 1777, 1848
BspQI GCTCTTC 1 cut(s) 1820
BspT104I TTCGAA 1 cut(s) 282
BspT107I GGYRCC 1 cut(s) 623
BsrFI RCCGGY 1 cut(s) 716
BsrI ACTGG 7 cut(s) 732, 1019, 1031, 1529, 1625, 1901, 1906
BssAI RCCGGY 1 cut(s) 716
BssECI CCNNGG 9 cut(s) 39, 315, 613, 614, 615, 635, 923, 1495, 1576
BssNI GRCGYC 1 cut(s) 303
BssSI CACGAG 1 cut(s) 1844
BssT1I CCWWGG 3 cut(s) 635, 923, 1576
Bst2BI CACGAG 1 cut(s) 1844
Bst2UI CCWGG 7 cut(s) 40, 374, 978, 1371, 1568, 1616, 1787
Bst4CI ACNGT 6 cut(s) 339, 905, 1410, 1549, 1759, 1777
Bst6I CTCTTC 4 cut(s) 86, 749, 1044, 1820
BstACI GRCGYC 1 cut(s) 303
BstBAI YACGTR 2 cut(s) 76, 1641
BstBI TTCGAA 1 cut(s) 282
BstC8I GCNNGC 1 cut(s) 798
BstDEI CTNAG 8 cut(s) 186, 507, 554, 687, 861, 971, 1383, 1742
BstF5I GGATG 3 cut(s) 933, 1040, 1662
BstH2I RGCGCY 1 cut(s) 1099
BstHHI GCGC 3 cut(s) 124, 805, 1098
BstMAI GTCTC 2 cut(s) 1236, 1268
BstMWI GCNNNNNNNGC 3 cut(s) 802, 1176, 1304
BstNI CCWGG 7 cut(s) 40, 374, 978, 1371, 1568, 1616, 1787
BstNSI RCATGY 4 cut(s) 138, 145, 349, 800
BstSFI CTRYAG 2 cut(s) 352, 1509
BstV1I GCAGC 4 cut(s) 812, 899, 1495, 1523
BstV2I GAAGAC 1 cut(s) 1672
BstX2I RGATCY 3 cut(s) 105, 376, 1782
BstXI CCANNNNNNTGG 2 cut(s) 203, 1673
BstYI RGATCY 3 cut(s) 105, 376, 1782
Bsu15I ATCGAT 1 cut(s) 181
BsuI GTATCC 1 cut(s) 1765
BsuRI GGCC 7 cut(s) 38, 320, 505, 976, 1003, 1321, 1747
BsuTUI ATCGAT 1 cut(s) 181
BtrI CACGTC 1 cut(s) 1162
BtsCI GGATG 3 cut(s) 933, 1040, 1662
BtsI GCAGTG 3 cut(s) 430, 1363, 1890
BtsIMutI CAGTG 6 cut(s) 430, 901, 1024, 1363, 1536, 1890
Cac8I GCNNGC 1 cut(s) 798
CfoI GCGC 3 cut(s) 124, 805, 1098
Cfr10I RCCGGY 1 cut(s) 716
Cfr13I GGNCC 2 cut(s) 1001, 1853
Cfr9I CCCGGG 1 cut(s) 614
ClaI ATCGAT 1 cut(s) 181
Csp6I GTAC 6 cut(s) 361, 624, 652, 1445, 1612, 1642
CviAII CATG 9 cut(s) 135, 142, 346, 369, 628, 797, 853, 1246, 1483
CviQI GTAC 6 cut(s) 361, 624, 652, 1445, 1612, 1642
DdeI CTNAG 8 cut(s) 186, 507, 554, 687, 861, 971, 1383, 1742
DraIII CACNNNGTG 1 cut(s) 341
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 4 cut(s) 86, 749, 1044, 1820
EarI CTCTTC 4 cut(s) 86, 749, 1044, 1820
EciI GGCGGA 1 cut(s) 465
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 3 cut(s) 635, 923, 1576
Eco147I AGGCCT 1 cut(s) 976
Eco24I GRGCYC 1 cut(s) 192
Eco32I GATATC 1 cut(s) 1466
Eco47I GGWCC 1 cut(s) 1853
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 2 cut(s) 1068, 1666
Eco88I CYCGRG 1 cut(s) 614
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 7 cut(s) 38, 372, 976, 1369, 1566, 1614, 1785
EcoRV GATATC 1 cut(s) 1466
EcoT14I CCWWGG 3 cut(s) 635, 923, 1576
EcoT22I ATGCAT 1 cut(s) 1172
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 3 cut(s) 635, 923, 1576
FaeI CATG 9 cut(s) 138, 145, 349, 372, 631, 800, 856, 1249, 1486
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FaqI GGGAC 2 cut(s) 1325, 1535
FatI CATG 9 cut(s) 134, 141, 345, 368, 627, 796, 852, 1245, 1482
FauI CCCGC 2 cut(s) 844, 917
Fnu4HI GCNGC 5 cut(s) 801, 913, 1004, 1509, 1512
FokI GGATG 3 cut(s) 940, 1047, 1649
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 5 cut(s) 801, 913, 1004, 1509, 1512
FspBI CTAG 3 cut(s) 1347, 1647, 1924
GlaI GCGC 3 cut(s) 123, 804, 1097
GluI GCNGC 5 cut(s) 801, 913, 1004, 1509, 1512
GsuI CTGGAG 2 cut(s) 1048, 1745
HaeII RGCGCY 1 cut(s) 1099
HaeIII GGCC 7 cut(s) 38, 320, 505, 976, 1003, 1321, 1747
HapII CCGG 6 cut(s) 231, 615, 717, 999, 1271, 1748
HhaI GCGC 3 cut(s) 124, 805, 1098
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 9 cut(s) 138, 145, 349, 372, 631, 800, 856, 1249, 1486
Hin6I GCGC 3 cut(s) 122, 803, 1096
HinP1I GCGC 3 cut(s) 122, 803, 1096
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HpaII CCGG 6 cut(s) 231, 615, 717, 999, 1271, 1748
HphI GGTGA 5 cut(s) 240, 608, 1126, 1801, 1916
Hpy166II GTNNAC 5 cut(s) 301, 307, 363, 961, 1853
Hpy188I TCNGA 6 cut(s) 187, 510, 701, 1216, 1470, 1743
Hpy188III TCNNGA 8 cut(s) 112, 515, 574, 766, 1120, 1223, 1694, 1844
Hpy8I GTNNAC 5 cut(s) 301, 307, 363, 961, 1853
Hpy99I CGWCG 2 cut(s) 1166, 1814
HpyAV CCTTC 5 cut(s) 378, 1105, 1177, 1408, 1745
HpyCH4III ACNGT 6 cut(s) 339, 905, 1410, 1549, 1759, 1777
HpyCH4IV ACGT 9 cut(s) 75, 218, 303, 1161, 1293, 1327, 1594, 1610, 1640
HpyF10VI GCNNNNNNNGC 3 cut(s) 802, 1176, 1304
HpyF3I CTNAG 8 cut(s) 186, 507, 554, 687, 861, 971, 1383, 1742
HpySE526I ACGT 9 cut(s) 75, 218, 303, 1161, 1293, 1327, 1594, 1610, 1640
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 9 cut(s) 138, 145, 349, 372, 631, 800, 856, 1249, 1486
HspAI GCGC 3 cut(s) 122, 803, 1096
KpnI GGTACC 1 cut(s) 627
LguI GCTCTTC 1 cut(s) 1820
LmnI GCTCC 4 cut(s) 149, 478, 641, 823
Lsp1109I GCAGC 4 cut(s) 812, 899, 1495, 1523
LweI GCATC 1 cut(s) 637
MaeI CTAG 3 cut(s) 1347, 1647, 1924
MaeII ACGT 9 cut(s) 75, 218, 303, 1161, 1293, 1327, 1594, 1610, 1640
MaeIII GTNAC 6 cut(s) 18, 137, 341, 596, 766, 1703
MfeI CAATTG 1 cut(s) 708
MflI RGATCY 3 cut(s) 105, 376, 1782
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 6 cut(s) 395, 577, 708, 1449, 1584, 1872
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 4 cut(s) 484, 520, 666, 1091
Mox20I TGGCCA 1 cut(s) 38
Mph1103I ATGCAT 1 cut(s) 1172
MroXI GAANNNNTTC 1 cut(s) 453
MscI TGGCCA 1 cut(s) 38
MseI TTAA 4 cut(s) 666, 1011, 1173, 1860
MslI CAYNNNNRTG 4 cut(s) 956, 1204, 1671, 1848
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 2 cut(s) 912, 1382
MspI CCGG 6 cut(s) 231, 615, 717, 999, 1271, 1748
MunI CAATTG 1 cut(s) 708
Mva1269I GAATGC 2 cut(s) 1172, 1492
MvaI CCWGG 7 cut(s) 40, 374, 978, 1371, 1568, 1616, 1787
MwoI GCNNNNNNNGC 3 cut(s) 802, 1176, 1304
NciI CCSGG 4 cut(s) 232, 615, 616, 1000
NlaIII CATG 9 cut(s) 138, 145, 349, 372, 631, 800, 856, 1249, 1486
NlaIV GGNNCC 2 cut(s) 378, 625
NmuCI GTSAC 4 cut(s) 341, 596, 766, 1703
NsiI ATGCAT 1 cut(s) 1172
NspI RCATGY 4 cut(s) 138, 145, 349, 800
NspV TTCGAA 1 cut(s) 282
OliI CACNNNNGTG 2 cut(s) 1671, 1848
PaeI GCATGC 1 cut(s) 800
PceI AGGCCT 1 cut(s) 976
PciI ACATGT 2 cut(s) 141, 345
PciSI GCTCTTC 1 cut(s) 1820
PctI GAATGC 2 cut(s) 1172, 1492
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 8 cut(s) 80, 583, 609, 791, 1073, 1350, 1374, 1738
PflFI GACNNNGTC 1 cut(s) 772
PflMI CCANNNNNTGG 1 cut(s) 1837
PfoI TCCNGGA 1 cut(s) 230
PkrI GCNGC 5 cut(s) 802, 914, 1005, 1510, 1513
PleI GAGTC 4 cut(s) 484, 519, 666, 1090
PpsI GAGTC 4 cut(s) 484, 519, 666, 1090
Ppu21I YACGTR 2 cut(s) 76, 1641
PscI ACATGT 2 cut(s) 141, 345
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 7 cut(s) 38, 372, 976, 1369, 1566, 1614, 1785
PspGI CCWGG 7 cut(s) 38, 372, 976, 1369, 1566, 1614, 1785
PspN4I GGNNCC 2 cut(s) 378, 625
PspPI GGNCC 2 cut(s) 1001, 1853
PstI CTGCAG 1 cut(s) 1513
PsuI RGATCY 3 cut(s) 105, 376, 1782
PsyI GACNNNGTC 1 cut(s) 772
PvuII CAGCTG 1 cut(s) 1382
RsaI GTAC 6 cut(s) 362, 625, 653, 1446, 1613, 1643
RsaNI GTAC 6 cut(s) 361, 624, 652, 1445, 1612, 1642
RseI CAYNNNNRTG 4 cut(s) 956, 1204, 1671, 1848
SacI GAGCTC 1 cut(s) 192
SapI GCTCTTC 1 cut(s) 1820
SaqAI TTAA 4 cut(s) 666, 1011, 1173, 1860
SatI GCNGC 5 cut(s) 801, 913, 1004, 1509, 1512
Sau96I GGNCC 2 cut(s) 1001, 1853
SchI GAGTC 4 cut(s) 484, 520, 666, 1091
SduI GDGCHC 1 cut(s) 192
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 2 cut(s) 352, 1509
SfuI TTCGAA 1 cut(s) 282
SinI GGWCC 1 cut(s) 1853
SmaI CCCGGG 1 cut(s) 616
SmiMI CAYNNNNRTG 4 cut(s) 956, 1204, 1671, 1848
SphI GCATGC 1 cut(s) 800
Sse9I AATT 6 cut(s) 395, 577, 708, 1449, 1584, 1872
SseBI AGGCCT 1 cut(s) 976
SsiI CCGC 5 cut(s) 266, 476, 837, 910, 1004
SspMI CTAG 3 cut(s) 1347, 1647, 1924
SstI GAGCTC 1 cut(s) 192
StuI AGGCCT 1 cut(s) 976
StyI CCWWGG 3 cut(s) 635, 923, 1576
TaaI ACNGT 6 cut(s) 339, 905, 1410, 1549, 1759, 1777
TaiI ACGT 9 cut(s) 78, 221, 306, 1164, 1296, 1330, 1597, 1613, 1643
TaqI TCGA 6 cut(s) 181, 282, 591, 891, 1119, 1164
TasI AATT 6 cut(s) 395, 577, 708, 1449, 1584, 1872
TatI WGTACW 1 cut(s) 360
TauI GCSGC 1 cut(s) 1006
TfiI GAWTC 8 cut(s) 80, 583, 609, 791, 1073, 1350, 1374, 1738
Tru1I TTAA 4 cut(s) 666, 1011, 1173, 1860
Tru9I TTAA 4 cut(s) 666, 1011, 1173, 1860
TscAI CASTG 6 cut(s) 437, 908, 1031, 1363, 1536, 1890
TseFI GTSAC 4 cut(s) 341, 596, 766, 1703
TseI GCWGC 4 cut(s) 800, 912, 1508, 1511
Tsp45I GTSAC 4 cut(s) 341, 596, 766, 1703
TspDTI ATGAA 4 cut(s) 228, 944, 1499, 1556
TspGWI ACGGA 3 cut(s) 684, 1382, 1827
TspMI CCCGGG 1 cut(s) 614
TspRI CASTG 6 cut(s) 437, 908, 1031, 1363, 1536, 1890
Tth111I GACNNNGTC 1 cut(s) 772
Van91I CCANNNNNTGG 1 cut(s) 1837
VpaK11BI GGWCC 1 cut(s) 1853
XapI RAATTY 2 cut(s) 395, 1872
XceI RCATGY 4 cut(s) 138, 145, 349, 800
XcmI CCANNNNNNNNNTGG 2 cut(s) 1230, 1574
XmaI CCCGGG 1 cut(s) 614
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 3 cut(s) 1347, 1647, 1924
ZraI GACGTC 1 cut(s) 304
Zsp2I ATGCAT 1 cut(s) 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.