MD06G1125900.v1.1

methyltransferase PMT18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004896
Physical Location & Seq
Reverse (-)
26788162 .. 26791237
3076 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1125900.v1.1.491

Sequence Viewer

Length: 1944 bp
ATGACCATGGATTGTAGTGGTGGATCGCCAAAGCACCATTACCTAGAATCCAAGAAGAAGAGGCTCACTTGGATCTTCGGGGTTAGTGCCCTCTGCATAGTATGTTACATGATAGGAGCTTGGCAAACCATTTCTGCCCCTGTCAATCAATCTGAGCTCTACCAAAGGGTCAGTTGTGATGAAACGTTATCTCAGTCCCGGAACAAAACTTCCTCATCCATGTCATCATCATCGCTTCACTTGGACTTCGAAAGCCATCATCAAGCGGACATCAACAAAACCAAGGCTGTTCAGAAGTTCCCACCGTGTGACATGTCCTACAGCGAGTACACTCCATGCCAGGATCCAACAAGGGGGAATAAATTCAACAGGAACATGTGGAAAAACAGAGAGAGGCATTGCCCTACCCAAGAAGAACAACTTTTTTGCCTTATTCCTGCTCCGCCAAAGTATAAGATCCCTTTCAAGTGGCCTCGGAGTCGTGATTTTGCTTGGTATGACAACATTCCCCATAGAGAACTCAGCATTGAGAAAGCTGGCCAGAATTGGATTCAAGTCATGGGTGACAGGTTTAGATTCCCCGGGGGAGGTACCATGTTTCGGAGGGGAGCTGATGCGTACATAGATGACATTAACGCGCTCATTCCTCTCACTAAGGGCAACATCAGAACTGCAATTGATACTGGCTGTGGTGTAGCCAGTTGGGGTGCTTACCTGTTGAAGAGGGACATCGTGACAATGTCTTTTGCGCCAAGAGATACGCACGAAGCACAGGTCCAGTTTGCATTGGAGCGCGGAGTTCCTGCTATGATTGGTGCCCTGGCTTCAAAGAGGCTTCCCTACCCTGCAAGAGCTTTCGATATGGCCCATTGTTCCCGCTGCTTGATCCCTTGGATGAACTATGGTAATGGTATGTATCTAATTGAAGTGGACAGAGTTCTAAGGCCTGGTGGTTATTGGATTCTTTCTGGGCCGCCTATTCACTGGAAAAAGAACTGGAGGGGATGGAACAGAACTCGAGAGGATTTAAAACAAGAGCAAGATTCTATTGAGTCTGTTGCAAAGCGCCTGTGCTGGAAGAAGGTGATCGAGAAGAACGATCTTGCAATCTGGCAAAAACCTATAAACCACGTCAAATGCGTTAAGAGCAGGAAGGTGTATAAAACACCACATATATGCAAATCAGACAATCCAGATATGGCTTGGGAAAGAGACATGGAGAGATGCATTACTCCTCTACAGGAGACCAGCAATCCCAATGACGTTGCTGGTGGGGCGTTGGAGAAATGGCCAGAGCGTGCATTTGCTGTCCCTCCTAGAATCAGCAGTGGTTCAATACCAGGAATCACTGCTGAGAAACTCCGCGAGGATAATCAACTGTGGAAAGAAAGGGTGGAACAATACAAGCGTATCATACCAATTTCCAATGGAAGGTATAGGAATGTGATGGACATGAATGCTTACCTCGGTGGATTTGCTGCAGCGTTGTCAAAATATCCAGTGTGGGTTATGAACACAGTCCCTGCCAACTCAAACCAGGACACTCTTGGAGTGATTTACGAACGGGGTTTCATTGGAACATACCAGGATTGGTGCGAGGCATTTTCAACTTATCCCAGAACATATGATCTCATCCACGCTGGGGGAGTCTTCAGCATATATCAGGACAGGTGTGACATAACACTCATTTTACTGGAGATGGATAGGATTCTGAGGCCGGAAGGAACTGTTGTGTTCAGGGATACTGTAGAGATCCTGGTGAAAATAAAGGCGATTACCGACGGAATGAGGTGGAAGAGCCAAATTATGGATCATGAAAGTGGACCTTGTGGCCACTTGGCCACATACTCCAACAGACCTTTTAATCCAGAGAAAATTCCTCTTGCTGAGAAAACTTACTGGACTGGCGAAGAAAAAAAAAGAAAGGTAGTAGAGATGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

648

Amino Acids

74.44

Weight (kDa)

9.03

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 109 - 609 1.1e-221 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 590
AccB1I GGYRCC 2 cut(s) 590, 815
AccB7I CCANNNNNTGG 1 cut(s) 1807
AccII CGCG 3 cut(s) 638, 795, 1365
AciI CCGC 6 cut(s) 266, 443, 795, 877, 974, 1363
AclI AACGTT 1 cut(s) 185
AclWI GGATC 8 cut(s) 31, 80, 338, 351, 451, 880, 1747, 1818
AcoI YGGCCR 4 cut(s) 538, 1289, 1831, 1839
AcsI RAATTY 2 cut(s) 362, 1875
AcuI CTGAAG 1 cut(s) 1636
AdeI CACNNNGTG 1 cut(s) 308
AfaI GTAC 3 cut(s) 329, 592, 620
AfiI CCNNNNNNNGG 6 cut(s) 353, 587, 600, 1431, 1642, 1807
AflIII ACRYGT 2 cut(s) 312, 375
AgsI TTSAA 8 cut(s) 367, 466, 554, 721, 828, 926, 1335, 1608
AjiI CACGTC 1 cut(s) 1132
AjnI CCWGG 7 cut(s) 339, 819, 946, 1339, 1536, 1584, 1755
AloI GAACNNNNNNTCC 2 cut(s) 194, 226
AluBI AGCT 5 cut(s) 119, 157, 536, 611, 854
AluI AGCT 5 cut(s) 119, 157, 536, 611, 854
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 2 cut(s) 1206, 1238
AlwI GGATC 8 cut(s) 31, 80, 338, 351, 451, 880, 1747, 1818
AlwNI CAGNNNCTG 1 cut(s) 1523
Ama87I CYCGRG 2 cut(s) 581, 1017
AoxI GGCC 9 cut(s) 470, 538, 864, 944, 971, 1289, 1715, 1831, 1839
ApeKI GCWGC 3 cut(s) 879, 1478, 1481
ApoI RAATTY 2 cut(s) 362, 1875
ArsI GACNNNNNNTTYG 2 cut(s) 759, 791
Asp700I GAANNNNTTC 1 cut(s) 362
Asp718I GGTACC 1 cut(s) 590
AspLEI GCGC 4 cut(s) 640, 751, 795, 1068
AspS9I GGNCC 4 cut(s) 775, 865, 971, 1823
AsuC2I CCSGG 3 cut(s) 199, 582, 583
AsuHPI GGTGA 3 cut(s) 575, 1096, 1771
AsuII TTCGAA 1 cut(s) 249
AvaI CYCGRG 2 cut(s) 581, 1017
AvaII GGWCC 2 cut(s) 775, 1823
BaeGI GKGCMC 2 cut(s) 91, 820
BaeI ACNNNNGTAYC 2 cut(s) 1393, 1426
BalI TGGCCA 4 cut(s) 540, 1291, 1833, 1841
BamHI GGATCC 1 cut(s) 343
BanI GGYRCC 2 cut(s) 590, 815
BanII GRGCYC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 1642
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 3 cut(s) 866, 1465, 1493
BccI CCATC 4 cut(s) 264, 999, 1441, 1693
BciT130I CCWGG 7 cut(s) 341, 821, 948, 1341, 1538, 1586, 1757
BciVI GTATCC 1 cut(s) 1735
BcnI CCSGG 3 cut(s) 199, 582, 583
BcoDI GTCTC 2 cut(s) 1206, 1238
BfaI CTAG 2 cut(s) 44, 1317
BfmI CTRYAG 4 cut(s) 319, 1238, 1479, 1746
BfoI RGCGCY 1 cut(s) 1069
BfuI GTATCC 1 cut(s) 1735
BisI GCNGC 4 cut(s) 880, 974, 1479, 1482
BlsI GCNGC 4 cut(s) 881, 975, 1480, 1483
Bme18I GGWCC 2 cut(s) 775, 1823
BmeT110I CYCGRG 2 cut(s) 581, 1017
BmgBI CACGTC 1 cut(s) 1132
BmgT120I GGNCC 4 cut(s) 775, 865, 971, 1823
BmiI GGNNCC 3 cut(s) 345, 592, 817
BmsI GCATC 2 cut(s) 604, 1214
BpiI GAAGAC 1 cut(s) 1642
BpmI CTGGAG 2 cut(s) 1018, 1715
Bpu14I TTCGAA 1 cut(s) 249
BpuMI CCSGG 3 cut(s) 199, 582, 583
BsaI GGTCTC 1 cut(s) 1238
BsaJI CCNNGG 9 cut(s) 6, 282, 473, 580, 581, 582, 819, 890, 1465
Bsc4I CCNNNNNNNGG 6 cut(s) 353, 587, 600, 1431, 1642, 1807
Bse1I ACTGG 9 cut(s) 688, 699, 778, 989, 1001, 1499, 1698, 1904, 1909
Bse3DI GCAATG 1 cut(s) 397
BseBI CCWGG 7 cut(s) 341, 821, 948, 1341, 1538, 1586, 1757
BseDI CCNNGG 9 cut(s) 6, 282, 473, 580, 581, 582, 819, 890, 1465
BseGI GGATG 4 cut(s) 215, 900, 1010, 1632
BseLI CCNNNNNNNGG 6 cut(s) 353, 587, 600, 1431, 1642, 1807
BseMI GCAATG 1 cut(s) 397
BseMII CTCAG 6 cut(s) 144, 206, 535, 1344, 1703, 1878
BseNI ACTGG 9 cut(s) 688, 699, 778, 989, 1001, 1499, 1698, 1904, 1909
BseRI GAGGAG 1 cut(s) 1224
BseSI GKGCMC 2 cut(s) 91, 820
BseXI GCAGC 3 cut(s) 866, 1465, 1493
BseYI CCCAGC 1 cut(s) 1640
Bsh1236I CGCG 3 cut(s) 638, 795, 1365
BshFI GGCC 9 cut(s) 472, 540, 866, 946, 973, 1291, 1717, 1833, 1841
BshNI GGYRCC 2 cut(s) 590, 815
BsiHKAI GWGCWC 1 cut(s) 159
BsiHKCI CYCGRG 2 cut(s) 581, 1017
BsiSI CCGG 3 cut(s) 199, 582, 1718
BslFI GGGAC 4 cut(s) 181, 740, 1295, 1505
BslI CCNNNNNNNGG 6 cut(s) 353, 587, 600, 1431, 1642, 1807
BsmAI GTCTC 2 cut(s) 1206, 1238
BsmFI GGGAC 4 cut(s) 181, 740, 1295, 1505
BsmI GAATGC 1 cut(s) 1462
BsnI GGCC 9 cut(s) 472, 540, 866, 946, 973, 1291, 1717, 1833, 1841
Bso31I GGTCTC 1 cut(s) 1238
BsoBI CYCGRG 2 cut(s) 581, 1017
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 3 cut(s) 91, 159, 820
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 6 cut(s) 266, 443, 795, 877, 974, 1363
BspANI GGCC 9 cut(s) 472, 540, 866, 946, 973, 1291, 1717, 1833, 1841
BspCNI CTCAG 6 cut(s) 145, 205, 534, 1345, 1704, 1879
BspFNI CGCG 3 cut(s) 638, 795, 1365
BspHI TCATGA 1 cut(s) 1813
BspLI GGNNCC 3 cut(s) 345, 592, 817
BspMAI CTGCAG 1 cut(s) 1483
BspPI GGATC 8 cut(s) 31, 80, 338, 351, 451, 880, 1747, 1818
BspQI GCTCTTC 1 cut(s) 1790
BspT104I TTCGAA 1 cut(s) 249
BspT107I GGYRCC 2 cut(s) 590, 815
BspTNI GGTCTC 1 cut(s) 1238
BsrDI GCAATG 1 cut(s) 397
BsrI ACTGG 9 cut(s) 688, 699, 778, 989, 1001, 1499, 1698, 1904, 1909
BssECI CCNNGG 9 cut(s) 6, 282, 473, 580, 581, 582, 819, 890, 1465
BssT1I CCWWGG 3 cut(s) 6, 282, 890
Bst2UI CCWGG 7 cut(s) 341, 821, 948, 1341, 1538, 1586, 1757
Bst4CI ACNGT 5 cut(s) 306, 1380, 1519, 1729, 1747
Bst6I CTCTTC 3 cut(s) 53, 716, 1790
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 2 cut(s) 538, 1299
BstDEI CTNAG 8 cut(s) 153, 192, 521, 654, 941, 1353, 1712, 1887
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 4 cut(s) 215, 900, 1010, 1632
BstFNI CGCG 3 cut(s) 638, 795, 1365
BstH2I RGCGCY 1 cut(s) 1069
BstHHI GCGC 4 cut(s) 640, 751, 795, 1068
BstMAI GTCTC 2 cut(s) 1206, 1238
BstMWI GCNNNNNNNGC 2 cut(s) 1146, 1274
BstNI CCWGG 7 cut(s) 341, 821, 948, 1341, 1538, 1586, 1757
BstNSI RCATGY 2 cut(s) 316, 379
BstSFI CTRYAG 4 cut(s) 319, 1238, 1479, 1746
BstSLI GKGCMC 2 cut(s) 91, 820
BstUI CGCG 3 cut(s) 638, 795, 1365
BstV1I GCAGC 3 cut(s) 866, 1465, 1493
BstV2I GAAGAC 1 cut(s) 1642
BstX2I RGATCY 4 cut(s) 72, 343, 456, 1752
BstYI RGATCY 4 cut(s) 72, 343, 456, 1752
BsuI GTATCC 1 cut(s) 1735
BsuRI GGCC 9 cut(s) 472, 540, 866, 946, 973, 1291, 1717, 1833, 1841
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 216
BtrI CACGTC 1 cut(s) 1132
BtsCI GGATG 4 cut(s) 215, 900, 1010, 1632
BtsI GCAGTG 2 cut(s) 1333, 1347
BtsIMutI CAGTG 4 cut(s) 982, 1333, 1347, 1506
Cac8I GCNNGC 2 cut(s) 538, 1299
CaiI CAGNNNCTG 1 cut(s) 1523
CciI TCATGA 1 cut(s) 1813
CfoI GCGC 4 cut(s) 640, 751, 795, 1068
Cfr13I GGNCC 4 cut(s) 775, 865, 971, 1823
Cfr9I CCCGGG 1 cut(s) 581
Csp6I GTAC 3 cut(s) 328, 591, 619
CviQI GTAC 3 cut(s) 328, 591, 619
DdeI CTNAG 8 cut(s) 153, 192, 521, 654, 941, 1353, 1712, 1887
DraI TTTAAA 1 cut(s) 1029
DraIII CACNNNGTG 1 cut(s) 308
EaeI YGGCCR 4 cut(s) 538, 1289, 1831, 1839
Eam1104I CTCTTC 3 cut(s) 53, 716, 1790
EarI CTCTTC 3 cut(s) 53, 716, 1790
EciI GGCGGA 1 cut(s) 432
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 3 cut(s) 6, 282, 890
Eco147I AGGCCT 1 cut(s) 946
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 1238
Eco47I GGWCC 2 cut(s) 775, 1823
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 1636
Eco88I CYCGRG 2 cut(s) 581, 1017
EcoICRI GAGCTC 1 cut(s) 157
EcoRII CCWGG 7 cut(s) 339, 819, 946, 1339, 1536, 1584, 1755
EcoT14I CCWWGG 3 cut(s) 6, 282, 890
EcoT22I ATGCAT 1 cut(s) 1229
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 3 cut(s) 6, 282, 890
FalI AAGNNNNNCTT 4 cut(s) 405, 437, 1810, 1842
FaqI GGGAC 4 cut(s) 181, 740, 1295, 1505
FauI CCCGC 1 cut(s) 884
FauNDI CATATG 1 cut(s) 1624
Fnu4HI GCNGC 4 cut(s) 880, 974, 1479, 1482
FokI GGATG 4 cut(s) 202, 907, 1017, 1619
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 4 cut(s) 880, 974, 1479, 1482
FspBI CTAG 2 cut(s) 44, 1317
GlaI GCGC 4 cut(s) 639, 750, 794, 1067
GluI GCNGC 4 cut(s) 880, 974, 1479, 1482
GsaI CCCAGC 1 cut(s) 1644
GsuI CTGGAG 2 cut(s) 1018, 1715
HaeII RGCGCY 1 cut(s) 1069
HaeIII GGCC 9 cut(s) 472, 540, 866, 946, 973, 1291, 1717, 1833, 1841
HapII CCGG 3 cut(s) 199, 582, 1718
HhaI GCGC 4 cut(s) 640, 751, 795, 1068
Hin6I GCGC 4 cut(s) 638, 749, 793, 1066
HinP1I GCGC 4 cut(s) 638, 749, 793, 1066
HpaII CCGG 3 cut(s) 199, 582, 1718
HphI GGTGA 3 cut(s) 575, 1096, 1771
Hpy166II GTNNAC 3 cut(s) 330, 931, 1823
Hpy188I TCNGA 7 cut(s) 154, 294, 477, 603, 668, 1186, 1713
Hpy188III TCNNGA 8 cut(s) 482, 733, 1019, 1090, 1193, 1664, 1814, 1868
Hpy8I GTNNAC 3 cut(s) 330, 931, 1823
Hpy99I CGWCG 1 cut(s) 1784
HpyAV CCTTC 4 cut(s) 1075, 1147, 1425, 1715
HpyCH4III ACNGT 5 cut(s) 306, 1380, 1519, 1729, 1747
HpyCH4IV ACGT 3 cut(s) 185, 1131, 1263
HpyF10VI GCNNNNNNNGC 2 cut(s) 1146, 1274
HpyF3I CTNAG 8 cut(s) 153, 192, 521, 654, 941, 1353, 1712, 1887
HpySE526I ACGT 3 cut(s) 185, 1131, 1263
HspAI GCGC 4 cut(s) 638, 749, 793, 1066
KpnI GGTACC 1 cut(s) 594
LguI GCTCTTC 1 cut(s) 1790
LmnI GCTCC 4 cut(s) 116, 445, 608, 790
Lsp1109I GCAGC 3 cut(s) 866, 1465, 1493
LweI GCATC 2 cut(s) 604, 1214
MaeI CTAG 2 cut(s) 44, 1317
MaeII ACGT 3 cut(s) 185, 1131, 1263
MaeIII GTNAC 5 cut(s) 104, 308, 563, 733, 1673
MfeI CAATTG 1 cut(s) 675
MflI RGATCY 4 cut(s) 72, 343, 456, 1752
MhlI GDGCHC 3 cut(s) 91, 159, 820
MlsI TGGCCA 4 cut(s) 540, 1291, 1833, 1841
MluCI AATT 7 cut(s) 362, 544, 675, 921, 1419, 1803, 1875
MluNI TGGCCA 4 cut(s) 540, 1291, 1833, 1841
MlyI GAGTC 3 cut(s) 487, 1061, 1656
MmeI TCCRAC 3 cut(s) 371, 1260, 1875
Mox20I TGGCCA 4 cut(s) 540, 1291, 1833, 1841
Mph1103I ATGCAT 1 cut(s) 1229
MroXI GAANNNNTTC 1 cut(s) 362
MscI TGGCCA 4 cut(s) 540, 1291, 1833, 1841
MseI TTAA 4 cut(s) 633, 1028, 1143, 1863
MslI CAYNNNNRTG 2 cut(s) 1174, 1818
Msp20I TGGCCA 4 cut(s) 540, 1291, 1833, 1841
MspA1I CMGCKG 1 cut(s) 879
MspI CCGG 3 cut(s) 199, 582, 1718
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 1462
MvaI CCWGG 7 cut(s) 341, 821, 948, 1341, 1538, 1586, 1757
MvnI CGCG 3 cut(s) 638, 795, 1365
MwoI GCNNNNNNNGC 2 cut(s) 1146, 1274
NciI CCSGG 3 cut(s) 199, 582, 583
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 1624
NlaIV GGNNCC 3 cut(s) 345, 592, 817
NmuCI GTSAC 4 cut(s) 308, 563, 733, 1673
NsiI ATGCAT 1 cut(s) 1229
NspI RCATGY 2 cut(s) 316, 379
NspV TTCGAA 1 cut(s) 249
PaeR7I CTCGAG 1 cut(s) 1017
PagI TCATGA 1 cut(s) 1813
PceI AGGCCT 1 cut(s) 946
PciI ACATGT 2 cut(s) 312, 375
PciSI GCTCTTC 1 cut(s) 1790
PcsI WCGNNNNNNNCGW 2 cut(s) 1095, 1137
PctI GAATGC 1 cut(s) 1462
PdmI GAANNNNTTC 1 cut(s) 362
PfeI GAWTC 8 cut(s) 47, 550, 576, 961, 1043, 1320, 1344, 1708
PflFI GACNNNGTC 1 cut(s) 739
PflMI CCANNNNNTGG 1 cut(s) 1807
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 4 cut(s) 881, 975, 1480, 1483
PleI GAGTC 3 cut(s) 486, 1060, 1655
PpsI GAGTC 3 cut(s) 486, 1060, 1655
PscI ACATGT 2 cut(s) 312, 375
Psp124BI GAGCTC 1 cut(s) 159
Psp1406I AACGTT 1 cut(s) 185
Psp6I CCWGG 7 cut(s) 339, 819, 946, 1339, 1536, 1584, 1755
PspFI CCCAGC 1 cut(s) 1640
PspGI CCWGG 7 cut(s) 339, 819, 946, 1339, 1536, 1584, 1755
PspN4I GGNNCC 3 cut(s) 345, 592, 817
PspPI GGNCC 4 cut(s) 775, 865, 971, 1823
PstI CTGCAG 1 cut(s) 1483
PstNI CAGNNNCTG 1 cut(s) 1523
PsuI RGATCY 4 cut(s) 72, 343, 456, 1752
PsyI GACNNNGTC 1 cut(s) 739
RsaI GTAC 3 cut(s) 329, 592, 620
RsaNI GTAC 3 cut(s) 328, 591, 619
RseI CAYNNNNRTG 2 cut(s) 1174, 1818
SacI GAGCTC 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 1790
SaqAI TTAA 4 cut(s) 633, 1028, 1143, 1863
SatI GCNGC 4 cut(s) 880, 974, 1479, 1482
Sau96I GGNCC 4 cut(s) 775, 865, 971, 1823
SchI GAGTC 3 cut(s) 487, 1061, 1656
SduI GDGCHC 3 cut(s) 91, 159, 820
SfaNI GCATC 2 cut(s) 604, 1214
SfcI CTRYAG 4 cut(s) 319, 1238, 1479, 1746
Sfr274I CTCGAG 1 cut(s) 1017
SfuI TTCGAA 1 cut(s) 249
SinI GGWCC 2 cut(s) 775, 1823
SlaI CTCGAG 1 cut(s) 1017
SmaI CCCGGG 1 cut(s) 583
SmiMI CAYNNNNRTG 2 cut(s) 1174, 1818
SmlI CTYRAG 1 cut(s) 1017
SmoI CTYRAG 1 cut(s) 1017
Sse9I AATT 7 cut(s) 362, 544, 675, 921, 1419, 1803, 1875
SseBI AGGCCT 1 cut(s) 946
SsiI CCGC 6 cut(s) 266, 443, 795, 877, 974, 1363
SspMI CTAG 2 cut(s) 44, 1317
SstI GAGCTC 1 cut(s) 159
StuI AGGCCT 1 cut(s) 946
StyI CCWWGG 3 cut(s) 6, 282, 890
TaaI ACNGT 5 cut(s) 306, 1380, 1519, 1729, 1747
TaiI ACGT 3 cut(s) 188, 1134, 1266
TaqI TCGA 4 cut(s) 249, 858, 1018, 1089
TasI AATT 7 cut(s) 362, 544, 675, 921, 1419, 1803, 1875
TatI WGTACW 1 cut(s) 327
TauI GCSGC 1 cut(s) 976
TfiI GAWTC 8 cut(s) 47, 550, 576, 961, 1043, 1320, 1344, 1708
Tru1I TTAA 4 cut(s) 633, 1028, 1143, 1863
Tru9I TTAA 4 cut(s) 633, 1028, 1143, 1863
TscAI CASTG 4 cut(s) 989, 1333, 1354, 1506
TseFI GTSAC 4 cut(s) 308, 563, 733, 1673
TseI GCWGC 3 cut(s) 879, 1478, 1481
Tsp45I GTSAC 4 cut(s) 308, 563, 733, 1673
TspDTI ATGAA 6 cut(s) 195, 911, 1469, 1526, 1561, 1830
TspGWI ACGGA 1 cut(s) 1797
TspMI CCCGGG 1 cut(s) 581
TspRI CASTG 4 cut(s) 989, 1333, 1354, 1506
Tth111I GACNNNGTC 1 cut(s) 739
Van91I CCANNNNNTGG 1 cut(s) 1807
VpaK11BI GGWCC 2 cut(s) 775, 1823
XapI RAATTY 2 cut(s) 362, 1875
XceI RCATGY 2 cut(s) 316, 379
XcmI CCANNNNNNNNNTGG 2 cut(s) 1200, 1544
XhoI CTCGAG 1 cut(s) 1017
XmaI CCCGGG 1 cut(s) 581
XmnI GAANNNNTTC 1 cut(s) 362
XspI CTAG 2 cut(s) 44, 1317
Zsp2I ATGCAT 1 cut(s) 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.