FvH4_5g11420

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003275
Physical Location & Seq
Forward (+)
6477141 .. 6481713
4573 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g11420.t5

Sequence Viewer

Length: 2241 bp
ATGGGGAGTTACAGTGAAGAGTGTGAGGATCAGTTTTATGATACCAGGGAGGACCTGTCTTCTGTGTCAGATTGGGGTGCAGATTGCTGTGAGGATTGTAGTCCTATTGCTTTGGTTTTTTCGAGGTATGAGTGTTGGAGTAAGAACCCTGAAAGTGTTCATGAGCGCCGCCATAGGTTTCTCAAATGGATGGGTTTGGATATAGATCGGAATTTGAGTATGACAGAAGAGTCAGATGATGGCTTCTTTGTTGACAGAAGTAGAACGGGTATTGATAGAATGAAAGAAGACAGTGGGGTGGTGTTGAGAAGTTCAGGTTTCGAAGAGGGGTGTTGCTCAAATCAGTGTACGTTGTCTTCTTTATCAAGTGAAGTTCGAGTATCACTAGGAAATGCTTTGGATGAGAATTTTGAATTTCCAACTAGGAAATTGAATGATCGAAGGGACTTTGTTGTGGATGAATTGCGTGGGGATGGAATACTCAGAAAATTCCGTGGGTTGAGACTAAACCAGTTGGTCAGTGCTGGGGAGATTCATTCCACCCGTGCACCTGCACCATCTCCTTCAATTCAGCAATTATTGCAGAGAGAAGTTGAAAATGCTAGGTACTTGCTTGATGCAAAAAAGACGGCTAAAAGAGGATGGTTAAAGAAGTTACGCATTGGAATGTGTGTTGCTGATAAACCTGGGGCTGCACTGAGCCCCATCAGTCTTAAAGCTGCAACACGGACAGGGATGCAAAGAGTCCGGGTTCAATCAAGTAAAAAGCGGAGCAAGGAACTGTCTTCTCTTTACGGTGGACAAAACTTTTCGGCTCATAAGGGGTCAATTTTGTCTATGAAGTTCAGTCTTGATGGGCAATACCTGGCCAGTGCTGGTCAAGATGGCATCGTCCGTGTATGGAAGGTGATTGAGGATGAGAGAATAGATAGATTTGATATTGCGAATGATCCTTCTTCTCTTTACTTCAAAATGAATCCATTTTCTAAGATAGGCTCTCAGGTTGTGGACAAAGAGAAACAAAGTAATGCAAAGAAATTGGGTGGATCATCGGACTCAGCTTGTGTGATTTTCCCTCCAAAGATTTTCCGCTTAACGGAGAAGCCTCTACATGAGTTCCGTGGACACAGTGGTGATGTCTTAGATCTCTCATGGTCAAAGAATGGGTTTCTGCTGTCATCCTCTGTTGATAAGACAGTTCGACTATGGCAAGTGGGATGTGACAGATGCCTGAGAGTCTTCTCTCATAATAATTATGTGACATGTGTTGATTTCAACCCTCTGGATGATAATTACTTTATCAGCGGTTCAATAGATGGAAAAGTTCGCATCTGGGAAATTATCCGTTGTCTGGTTGTGGATTATATTGACGTCCGCGAGATAGTCACTGCTGTATCTTATTGTCCTGATGGAAAGGGAGGAATTGTGGGCTTCGTCACCGGAAACTGCTGCTTTTTTAATATTATAGATGATCACTTGAAATTGGATGCTCAGATATGCATACAGGGTAAAAAGAAGTCGGTGGGAAAGAGGATTACTGGTTTTCAGTTTTCTCCAAGCAACCCAAGAAAAGTTATGGTCACCTCTGCCGATTCAGTAATACGTGTCATTTCTGGGACTAAGGTCATCTGCAAATTTAAGGGCCTACGAAGTGGAGGAAGCCATGTTTCTGCATCTTTTACCTCGGATGGAAACCACATTGTTTCGGCAAGTGAGGATTCAAGTGTTCACATCTGGAACTACAATGGCCAGGAGACATCCTCTTCACGATCAAAGAACATAAGGTCTTGTGAGAGTTTTGTGTCCCACAACTCGATGATCGCTATACCTTGGAATGGTGTGAATGACCTTCCAGCAATGCTTCCATCCCCTGCATTCATCGGGGATTCACAGGGAAGAAGTAGCCTTGATAGTCCGCTGAAGCTTCTCAATTTCGACGAGAGAGTACAACAGAAAATGCATCTCTCTTCACCTGATTGCTTCTCTCTGGGTCGTGGGTTTTTGTTGGAGTCATTGCCAAAGGGTACTCCAACTTGGCCCGAAGAAAAGCTTGTGAACTCAAGTCCCGTGCCCCCGGTGCCCGTTTCCCCAACAATGTCAAGATCAGAGTACAGGTTCTTGAAGAATGCATGTCAGAGCTTGTCTTCTTCTTCTCATATGTGGGGCCTCGTGATTGTCACAGCCGGCTTGGATGGACGTATTAGAACTTACCACAATTATGGGTTACCAACTCGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

747

Amino Acids

83.13

Weight (kDa)

8.63

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 268 - 446 3.2e-12 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 268 - 319 1.3e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 268 - 312 5.5e-06 WDR3 first beta-propeller domain
WD40 PF00400 269 - 301 1.2e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 269 - 323 1.8e-07 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 269 - 318 6.8e-07 WDHD1 first WD40 domain
WD40_Gbeta PF25391 271 - 580 5.6e-09 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 332 - 472 6.8e-13 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 349 - 586 2.6e-22 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 349 - 447 1e-12 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 361 - 589 5.7e-32 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 367 - 430 3.2e-07 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 367 - 535 2.4e-11 CDC20/Fizzy WD40 domain
WD40 PF00400 368 - 403 2.1e-08 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 372 - 450 1.5e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 379 - 580 4.3e-11 WDR3 second beta-propeller domain
WD40 PF00400 409 - 445 7.3e-07 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 424 - 614 1.1e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 517 - 613 2.1e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 559
AasI GACNNNNNNGTC 1 cut(s) 229
AatII GACGTC 1 cut(s) 1374
Acc36I ACCTGC 1 cut(s) 559
AccB1I GGYRCC 1 cut(s) 2077
AccII CGCG 1 cut(s) 1377
AciI CCGC 6 cut(s) 169, 769, 1090, 1305, 1375, 1916
AclWI GGATC 3 cut(s) 36, 944, 1054
AcoI YGGCCR 2 cut(s) 867, 1747
AcsI RAATTY 5 cut(s) 211, 406, 413, 488, 1634
AcuI CTGAAG 1 cut(s) 1940
AcyI GRCGYC 1 cut(s) 1371
AfaI GTAC 5 cut(s) 349, 608, 1947, 2026, 2111
AfiI CCNNNNNNNGG 3 cut(s) 1096, 1351, 1835
AflIII ACRYGT 2 cut(s) 1262, 1603
AjnI CCWGG 4 cut(s) 44, 685, 864, 1749
AleI CACNNNNGTG 1 cut(s) 1131
AluBI AGCT 5 cut(s) 719, 1061, 1924, 2050, 2139
AluI AGCT 5 cut(s) 719, 1061, 1924, 2050, 2139
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 2 cut(s) 496, 1748
Alw44I GTGCAC 1 cut(s) 546
AlwI GGATC 3 cut(s) 36, 944, 1054
AoxI GGCC 5 cut(s) 867, 1642, 1747, 2036, 2164
ApaLI GTGCAC 1 cut(s) 546
ApeKI GCWGC 3 cut(s) 692, 719, 1449
ApoI RAATTY 5 cut(s) 211, 406, 413, 488, 1634
Asp700I GAANNNNTTC 1 cut(s) 156
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 4 cut(s) 52, 1642, 2037, 2164
AsuC2I CCSGG 2 cut(s) 749, 2075
AsuHPI GGTGA 5 cut(s) 919, 1145, 1429, 1573, 1962
AsuII TTCGAA 1 cut(s) 321
AvaII GGWCC 1 cut(s) 52
BaeGI GKGCMC 3 cut(s) 550, 2073, 2082
BaeI ACNNNNGTAYC 2 cut(s) 1377, 1410
BalI TGGCCA 2 cut(s) 869, 1749
BanI GGYRCC 1 cut(s) 2077
BanII GRGCYC 1 cut(s) 704
BarI GAAGNNNNNNTAC 2 cut(s) 340, 372
BauI CACGAG 1 cut(s) 2168
BbsI GAAGAC 6 cut(s) 51, 294, 348, 777, 1231, 2136
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 3 cut(s) 679, 706, 1436
BceAI ACGGC 1 cut(s) 645
BciT130I CCWGG 4 cut(s) 46, 687, 866, 1751
BclI TGATCA 1 cut(s) 1471
BcnI CCSGG 2 cut(s) 749, 2075
BcoDI GTCTC 2 cut(s) 496, 1748
BfaI CTAG 3 cut(s) 386, 423, 603
BfoI RGCGCY 1 cut(s) 169
BfuAI ACCTGC 1 cut(s) 559
BglII AGATCT 1 cut(s) 1144
BisI GCNGC 4 cut(s) 169, 693, 720, 1450
BlsI GCNGC 4 cut(s) 170, 694, 721, 1451
Bme1390I CCNGG 6 cut(s) 46, 687, 749, 866, 1751, 2075
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 4 cut(s) 52, 1642, 2037, 2164
BmiI GGNNCC 2 cut(s) 2079, 2165
BmrFI CCNGG 6 cut(s) 46, 687, 749, 866, 1751, 2075
BmsI GCATC 8 cut(s) 607, 726, 897, 1217, 1338, 1477, 1682, 1969
BoxI GACNNNNGTC 1 cut(s) 1622
BpiI GAAGAC 6 cut(s) 51, 294, 348, 777, 1231, 2136
BplI GAGNNNNNCTC 2 cut(s) 1745, 1777
Bpu14I TTCGAA 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 2044
BpuMI CCSGG 2 cut(s) 749, 2075
BsaAI YACGTR 1 cut(s) 1604
BsaBI GATNNNNATC 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 1371
BsaJI CCNNGG 7 cut(s) 45, 493, 686, 1120, 1683, 1829, 2073
BsaWI WCCGGW 1 cut(s) 1439
BsaXI ACNNNNNCTCC 5 cut(s) 28, 41, 71, 1410, 1440
Bsc4I CCNNNNNNNGG 3 cut(s) 1096, 1351, 1835
Bse118I RCCGGY 1 cut(s) 2183
Bse1I ACTGG 3 cut(s) 511, 870, 1543
Bse3DI GCAATG 2 cut(s) 1863, 2012
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 4 cut(s) 46, 687, 866, 1751
BseDI CCNNGG 7 cut(s) 45, 493, 686, 1120, 1683, 1829, 2073
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 3 cut(s) 1096, 1351, 1835
BseMI GCAATG 2 cut(s) 1863, 2012
BseMII CTCAG 6 cut(s) 496, 689, 1013, 1071, 1223, 1505
BseNI ACTGG 3 cut(s) 511, 870, 1543
BseSI GKGCMC 3 cut(s) 550, 2073, 2082
BseXI GCAGC 3 cut(s) 679, 706, 1436
BseYI CCCAGC 1 cut(s) 524
BsgI GTGCAG 3 cut(s) 99, 537, 678
Bsh1236I CGCG 1 cut(s) 1377
BshFI GGCC 5 cut(s) 869, 1644, 1749, 2038, 2166
BshNI GGYRCC 1 cut(s) 2077
BsiHKAI GWGCWC 1 cut(s) 550
BsiSI CCGG 4 cut(s) 748, 1440, 2075, 2184
BslFI GGGAC 4 cut(s) 458, 1630, 1789, 2049
BslI CCNNNNNNNGG 3 cut(s) 1096, 1351, 1835
BsmAI GTCTC 2 cut(s) 496, 1748
BsmFI GGGAC 4 cut(s) 458, 1630, 1789, 2049
BsmI GAATGC 2 cut(s) 1874, 2131
BsnI GGCC 5 cut(s) 869, 1644, 1749, 2038, 2166
Bsp119I TTCGAA 1 cut(s) 321
Bsp1286I GDGCHC 4 cut(s) 550, 704, 2073, 2082
BspACI CCGC 6 cut(s) 169, 769, 1090, 1305, 1375, 1916
BspANI GGCC 5 cut(s) 869, 1644, 1749, 2038, 2166
BspCNI CTCAG 6 cut(s) 495, 690, 1012, 1070, 1224, 1504
BspFNI CGCG 1 cut(s) 1377
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 2079, 2165
BspMI ACCTGC 1 cut(s) 559
BspPI GGATC 3 cut(s) 36, 944, 1054
BspT104I TTCGAA 1 cut(s) 321
BspT107I GGYRCC 1 cut(s) 2077
BsrDI GCAATG 2 cut(s) 1863, 2012
BsrFI RCCGGY 1 cut(s) 2183
BsrI ACTGG 3 cut(s) 511, 870, 1543
BssAI RCCGGY 1 cut(s) 2183
BssECI CCNNGG 7 cut(s) 45, 493, 686, 1120, 1683, 1829, 2073
BssNI GRCGYC 1 cut(s) 1371
BssSI CACGAG 1 cut(s) 2168
BssT1I CCWWGG 1 cut(s) 1829
Bst2BI CACGAG 1 cut(s) 2168
Bst2UI CCWGG 4 cut(s) 46, 687, 866, 1751
Bst4CI ACNGT 6 cut(s) 14, 293, 783, 797, 1130, 1198
Bst6I CTCTTC 5 cut(s) 12, 222, 318, 1768, 1972
BstACI GRCGYC 1 cut(s) 1371
BstAPI GCANNNNNTGC 1 cut(s) 580
BstBAI YACGTR 1 cut(s) 1604
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 1 cut(s) 2185
BstDEI CTNAG 9 cut(s) 482, 698, 987, 999, 1057, 1141, 1232, 1491, 1620
BstDSI CCRYGG 2 cut(s) 493, 1120
BstEII GGTNACC 2 cut(s) 1579, 2223
BstFNI CGCG 1 cut(s) 1377
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstMAI GTCTC 2 cut(s) 496, 1748
BstMWI GCNNNNNNNGC 2 cut(s) 580, 2077
BstNI CCWGG 4 cut(s) 46, 687, 866, 1751
BstNSI RCATGY 2 cut(s) 1266, 2133
BstPAI GACNNNNGTC 1 cut(s) 1622
BstPI GGTNACC 2 cut(s) 1579, 2223
BstSCI CCNGG 6 cut(s) 44, 685, 747, 864, 1749, 2073
BstSLI GKGCMC 3 cut(s) 550, 2073, 2082
BstUI CGCG 1 cut(s) 1377
BstV1I GCAGC 3 cut(s) 679, 706, 1436
BstV2I GAAGAC 6 cut(s) 51, 294, 348, 777, 1231, 2136
BstX2I RGATCY 1 cut(s) 1144
BstXI CCANNNNNNTGG 1 cut(s) 2219
BstYI RGATCY 1 cut(s) 1144
BsuRI GGCC 5 cut(s) 869, 1644, 1749, 2038, 2166
BtgI CCRYGG 2 cut(s) 493, 1120
BtsI GCAGTG 1 cut(s) 1386
BtsIMutI CAGTG 8 cut(s) 19, 298, 350, 526, 695, 877, 1135, 1386
BveI ACCTGC 1 cut(s) 559
Cac8I GCNNGC 1 cut(s) 2185
CciI TCATGA 1 cut(s) 160
CfoI GCGC 1 cut(s) 168
Cfr10I RCCGGY 1 cut(s) 2183
Cfr13I GGNCC 4 cut(s) 52, 1642, 2037, 2164
Csp6I GTAC 5 cut(s) 348, 607, 1946, 2025, 2110
CviAII CATG 6 cut(s) 161, 1112, 1152, 1263, 1664, 2130
CviQI GTAC 5 cut(s) 348, 607, 1946, 2025, 2110
DdeI CTNAG 9 cut(s) 482, 698, 987, 999, 1057, 1141, 1232, 1491, 1620
DrdI GACNNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 2 cut(s) 867, 1747
Eam1104I CTCTTC 5 cut(s) 12, 222, 318, 1768, 1972
EarI CTCTTC 5 cut(s) 12, 222, 318, 1768, 1972
Eco130I CCWWGG 1 cut(s) 1829
Eco24I GRGCYC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 1940
Eco91I GGTNACC 2 cut(s) 1579, 2223
EcoO109I RGGNCCY 3 cut(s) 52, 1642, 2164
EcoO65I GGTNACC 2 cut(s) 1579, 2223
EcoRII CCWGG 4 cut(s) 44, 685, 864, 1749
EcoT14I CCWWGG 1 cut(s) 1829
EcoT22I ATGCAT 3 cut(s) 1502, 1962, 2131
EcoT38I GRGCYC 1 cut(s) 704
ErhI CCWWGG 1 cut(s) 1829
FaeI CATG 6 cut(s) 164, 1115, 1155, 1266, 1667, 2133
FalI AAGNNNNNCTT 2 cut(s) 2034, 2066
FaqI GGGAC 4 cut(s) 458, 1630, 1789, 2049
FatI CATG 6 cut(s) 160, 1111, 1151, 1262, 1663, 2129
FauNDI CATATG 1 cut(s) 2157
FbaI TGATCA 1 cut(s) 1471
Fnu4HI GCNGC 4 cut(s) 169, 693, 720, 1450
FriOI GRGCYC 1 cut(s) 704
Fsp4HI GCNGC 4 cut(s) 169, 693, 720, 1450
FspBI CTAG 3 cut(s) 386, 423, 603
GlaI GCGC 1 cut(s) 167
GluI GCNGC 4 cut(s) 169, 693, 720, 1450
GsaI CCCAGC 1 cut(s) 528
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 5 cut(s) 869, 1644, 1749, 2038, 2166
HapII CCGG 4 cut(s) 748, 1440, 2075, 2184
HhaI GCGC 1 cut(s) 168
Hin1I GRCGYC 1 cut(s) 1371
Hin1II CATG 6 cut(s) 164, 1115, 1155, 1266, 1667, 2133
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 2 cut(s) 1922, 2048
HpaII CCGG 4 cut(s) 748, 1440, 2075, 2184
HphI GGTGA 5 cut(s) 919, 1145, 1429, 1573, 1962
Hpy166II GTNNAC 8 cut(s) 253, 348, 548, 800, 1009, 1124, 1729, 2056
Hpy188I TCNGA 9 cut(s) 70, 210, 235, 485, 1054, 1494, 1687, 2107, 2136
Hpy8I GTNNAC 8 cut(s) 253, 348, 548, 800, 1009, 1124, 1729, 2056
Hpy99I CGWCG 1 cut(s) 1940
HpyAV CCTTC 5 cut(s) 435, 573, 898, 963, 1859
HpyCH4III ACNGT 6 cut(s) 14, 293, 783, 797, 1130, 1198
HpyCH4IV ACGT 4 cut(s) 350, 1371, 1603, 2197
HpyF10VI GCNNNNNNNGC 2 cut(s) 580, 2077
HpyF3I CTNAG 9 cut(s) 482, 698, 987, 999, 1057, 1141, 1232, 1491, 1620
HpySE526I ACGT 4 cut(s) 350, 1371, 1603, 2197
Hsp92I GRCGYC 1 cut(s) 1371
Hsp92II CATG 6 cut(s) 164, 1115, 1155, 1266, 1667, 2133
HspAI GCGC 1 cut(s) 166
KroI GCCGGC 1 cut(s) 2183
KroNI GCCGGC 1 cut(s) 2185
Ksp22I TGATCA 1 cut(s) 1471
LmnI GCTCC 1 cut(s) 771
Lsp1109I GCAGC 3 cut(s) 679, 706, 1436
LweI GCATC 8 cut(s) 607, 726, 897, 1217, 1338, 1477, 1682, 1969
MaeI CTAG 3 cut(s) 386, 423, 603
MaeII ACGT 4 cut(s) 350, 1371, 1603, 2197
MaeIII GTNAC 9 cut(s) 8, 654, 1220, 1258, 1384, 1435, 1579, 2176, 2223
MflI RGATCY 1 cut(s) 1144
MhlI GDGCHC 4 cut(s) 550, 704, 2073, 2082
MlsI TGGCCA 2 cut(s) 869, 1749
MluNI TGGCCA 2 cut(s) 869, 1749
MlyI GAGTC 5 cut(s) 239, 753, 1049, 1245, 2018
MmeI TCCRAC 4 cut(s) 116, 443, 1986, 2054
Mox20I TGGCCA 2 cut(s) 869, 1749
Mph1103I ATGCAT 3 cut(s) 1502, 1962, 2131
MroNI GCCGGC 1 cut(s) 2183
MroXI GAANNNNTTC 1 cut(s) 156
MscI TGGCCA 2 cut(s) 869, 1749
MseI TTAA 6 cut(s) 647, 714, 1094, 1458, 1638, 2239
MslI CAYNNNNRTG 3 cut(s) 665, 1131, 2217
Msp20I TGGCCA 2 cut(s) 869, 1749
MspA1I CMGCKG 2 cut(s) 1305, 1918
MspI CCGG 4 cut(s) 748, 1440, 2075, 2184
MspR9I CCNGG 6 cut(s) 46, 687, 749, 866, 1751, 2075
Mva1269I GAATGC 2 cut(s) 1874, 2131
MvaI CCWGG 4 cut(s) 46, 687, 866, 1751
MvnI CGCG 1 cut(s) 1377
MwoI GCNNNNNNNGC 2 cut(s) 580, 2077
NaeI GCCGGC 1 cut(s) 2185
NciI CCSGG 2 cut(s) 749, 2075
NdeI CATATG 1 cut(s) 2157
NgoMIV GCCGGC 1 cut(s) 2183
NlaIII CATG 6 cut(s) 164, 1115, 1155, 1266, 1667, 2133
NlaIV GGNNCC 2 cut(s) 2079, 2165
NmuCI GTSAC 6 cut(s) 1220, 1258, 1384, 1435, 1579, 2176
NsiI ATGCAT 3 cut(s) 1502, 1962, 2131
NspI RCATGY 2 cut(s) 1266, 2133
NspV TTCGAA 1 cut(s) 321
OliI CACNNNNGTG 1 cut(s) 1131
PagI TCATGA 1 cut(s) 160
PaqCI CACCTGC 1 cut(s) 559
PciI ACATGT 1 cut(s) 1262
PctI GAATGC 2 cut(s) 1874, 2131
PdiI GCCGGC 1 cut(s) 2185
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 5 cut(s) 532, 976, 1592, 1718, 1886
PkrI GCNGC 4 cut(s) 170, 694, 721, 1451
PleI GAGTC 5 cut(s) 238, 752, 1049, 1244, 2017
PpsI GAGTC 5 cut(s) 238, 752, 1049, 1244, 2017
Ppu21I YACGTR 1 cut(s) 1604
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 1 cut(s) 1262
PshAI GACNNNNGTC 1 cut(s) 1622
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 4 cut(s) 44, 685, 864, 1749
PspEI GGTNACC 2 cut(s) 1579, 2223
PspFI CCCAGC 1 cut(s) 524
PspGI CCWGG 4 cut(s) 44, 685, 864, 1749
PspN4I GGNNCC 2 cut(s) 2079, 2165
PspPI GGNCC 4 cut(s) 52, 1642, 2037, 2164
PspPPI RGGWCCY 1 cut(s) 52
PsuI RGATCY 1 cut(s) 1144
RsaI GTAC 5 cut(s) 349, 608, 1947, 2026, 2111
RsaNI GTAC 5 cut(s) 348, 607, 1946, 2025, 2110
RseI CAYNNNNRTG 3 cut(s) 665, 1131, 2217
SaqAI TTAA 6 cut(s) 647, 714, 1094, 1458, 1638, 2239
SatI GCNGC 4 cut(s) 169, 693, 720, 1450
Sau96I GGNCC 4 cut(s) 52, 1642, 2037, 2164
SchI GAGTC 5 cut(s) 239, 753, 1049, 1245, 2018
ScrFI CCNGG 6 cut(s) 46, 687, 749, 866, 1751, 2075
SduI GDGCHC 4 cut(s) 550, 704, 2073, 2082
SfaNI GCATC 8 cut(s) 607, 726, 897, 1217, 1338, 1477, 1682, 1969
SfuI TTCGAA 1 cut(s) 321
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 3 cut(s) 665, 1131, 2217
SmlI CTYRAG 1 cut(s) 2059
SmoI CTYRAG 1 cut(s) 2059
SsiI CCGC 6 cut(s) 169, 769, 1090, 1305, 1375, 1916
SspI AATATT 1 cut(s) 1462
SspMI CTAG 3 cut(s) 386, 423, 603
StyD4I CCNGG 6 cut(s) 44, 685, 747, 864, 1749, 2073
StyI CCWWGG 1 cut(s) 1829
TaaI ACNGT 6 cut(s) 14, 293, 783, 797, 1130, 1198
TaiI ACGT 4 cut(s) 353, 1374, 1606, 2200
TaqI TCGA 7 cut(s) 122, 321, 376, 439, 1201, 1814, 1935
TatI WGTACW 2 cut(s) 1945, 2109
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 5 cut(s) 532, 976, 1592, 1718, 1886
Tru1I TTAA 6 cut(s) 647, 714, 1094, 1458, 1638, 2239
Tru9I TTAA 6 cut(s) 647, 714, 1094, 1458, 1638, 2239
TscAI CASTG 8 cut(s) 19, 298, 350, 526, 702, 877, 1135, 1393
TseFI GTSAC 6 cut(s) 1220, 1258, 1384, 1435, 1579, 2176
TseI GCWGC 3 cut(s) 692, 719, 1449
Tsp45I GTSAC 6 cut(s) 1220, 1258, 1384, 1435, 1579, 2176
TspDTI ATGAA 7 cut(s) 149, 296, 474, 524, 854, 989, 1867
TspGWI ACGGA 6 cut(s) 482, 742, 884, 1109, 1112, 1334
TspRI CASTG 8 cut(s) 19, 298, 350, 526, 702, 877, 1135, 1393
VneI GTGCAC 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 52
XapI RAATTY 5 cut(s) 211, 406, 413, 488, 1634
XceI RCATGY 2 cut(s) 1266, 2133
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 3 cut(s) 386, 423, 603
ZraI GACGTC 1 cut(s) 1372
Zsp2I ATGCAT 3 cut(s) 1502, 1962, 2131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.