Rorug06G0474300

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003275
Physical Location & Seq
Reverse (-)
59713674 .. 59718063
4390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0474300.1

Sequence Viewer

Length: 1017 bp
ATGGCTCTTCCCCAACACCACTTTCAACAACACTATCCGCCCCAACAACAACAGCAGCAATCCAAGTCATTCAGAAATTTATACACAATCGACGGTCAGATATCGCCGGCGGTGGCCTATTTCGACTCCAACGCCTTACAAGATCACTCCCAGCATCCGCCTTATGTTCCACCCTTTCATGTAGTAGGGTTTGCGCCTGGTCCCGTGGCTGCAACAGACGGCAGTGACAATGGTGCTGATTTTCAATGGAACTATGGCTTAGAATCCAAGAGGAAAAGGCCCAAGGAGCAGGATTTTTTGGAGAACAACTCGCAAATGTCTTCGGTTCTGCAACAGCAGCAATCAGTCTCAACGGGCTTGGGTCTGTCCCTTGACAATAATCGCATGGCGTCTACAGGGGACTCGGCTTTGCTGTCTCTCATTGGCGAGGATGTTGATCTCGAGATACAGCGGCAAGATGTTGAGATTGATAGGTTCCTCAAAGCCCAGAGTGATCGATTGCGACAAAATATCCTGGACAAGGTCCAAGCTGCCCAACTTCAAACTCTCTCAGTTGTAGAAGAAAAAGTACTCCAGAAGCTCCGGGAAAAAGAAGCAGAAGTCGAGAGCATCAACAAGAAGAATATGGAGCTTGAAGAAAGAATGGATCAGTTGAGTCTTGAAGCAAGGATTTGGCAACATGAAGCCAGGAACAATGAACACATGATCACAGCTCTCAAGGTTAATCTTGATCAAGTAATTGCTCAAAGTAGAGACAGCAAGGAAGGATGTGGTGACAGTGAAATAGATGATACAGCTTCCTGCTGCAATGGCCATTCCATCAATTTTAATATGTTTGGCAAGGAGAGCAATGATGTGAAAGAGGTAATGACGTGCAAGGCCTGCAGAGTGAATGAAGTTTGCATGCTTCTATTACCCTGTAAGCATCTTTGCCTGTGTAAAGATTGTGAATGTAAGCTTAGCATTTGTCCTGTGTGTCAGTCCTCTAAGTTTATCGGCATGGAAGTATACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

338

Amino Acids

38.22

Weight (kDa)

5.19

Isoelectric Point (pI)

57.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 290 - 328 8.6e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 392, 1008
AciI CCGC 4 cut(s) 38, 110, 158, 451
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 1 cut(s) 76
AcyI GRCGYC 1 cut(s) 389
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 1 cut(s) 520
AgsI TTSAA 5 cut(s) 26, 245, 542, 635, 662
AjiI CACGTC 1 cut(s) 873
AjnI CCWGG 3 cut(s) 196, 513, 686
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AluBI AGCT 6 cut(s) 530, 580, 631, 713, 797, 958
AluI AGCT 6 cut(s) 530, 580, 631, 713, 797, 958
Alw26I GTCTC 3 cut(s) 352, 420, 747
AlwI GGATC 1 cut(s) 654
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 4 cut(s) 114, 278, 811, 879
ApeKI GCWGC 5 cut(s) 55, 209, 337, 530, 804
ApoI RAATTY 1 cut(s) 76
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 3 cut(s) 200, 279, 523
AsuC2I CCSGG 1 cut(s) 584
AsuHPI GGTGA 1 cut(s) 785
AvaI CYCGRG 1 cut(s) 440
AvaII GGWCC 2 cut(s) 200, 523
BalI TGGCCA 1 cut(s) 813
BarI GAAGNNNNNNTAC 2 cut(s) 552, 584
BbsI GAAGAC 1 cut(s) 312
BbvI GCAGC 5 cut(s) 67, 196, 349, 517, 791
BccI CCATC 1 cut(s) 827
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 3 cut(s) 198, 515, 688
BclI TGATCA 2 cut(s) 705, 730
BcnI CCSGG 1 cut(s) 584
BcoDI GTCTC 3 cut(s) 352, 420, 747
BfmI CTRYAG 2 cut(s) 393, 883
BisI GCNGC 6 cut(s) 56, 210, 338, 452, 531, 805
BlpI GCTNAGC 1 cut(s) 959
BlsI GCNGC 6 cut(s) 57, 211, 339, 453, 532, 806
BmcAI AGTACT 1 cut(s) 570
Bme1390I CCNGG 4 cut(s) 198, 515, 584, 688
Bme18I GGWCC 2 cut(s) 200, 523
BmeT110I CYCGRG 1 cut(s) 440
BmgBI CACGTC 1 cut(s) 873
BmgT120I GGNCC 3 cut(s) 200, 279, 523
BmiI GGNNCC 2 cut(s) 202, 476
BmrFI CCNGG 4 cut(s) 198, 515, 584, 688
BmsI GCATC 3 cut(s) 163, 618, 934
BpiI GAAGAC 1 cut(s) 312
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpmI CTGGAG 1 cut(s) 557
Bpu1102I GCTNAGC 1 cut(s) 959
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 1 cut(s) 584
Bsa29I ATCGAT 1 cut(s) 496
BsaBI GATNNNNATC 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 389
BsaJI CCNNGG 2 cut(s) 204, 282
Bsc4I CCNNNNNNNGG 1 cut(s) 520
Bse118I RCCGGY 1 cut(s) 106
Bse3DI GCAATG 2 cut(s) 814, 856
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 3 cut(s) 198, 515, 688
BseCI ATCGAT 1 cut(s) 496
BseDI CCNNGG 2 cut(s) 204, 282
BseGI GGATG 3 cut(s) 154, 436, 773
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 1 cut(s) 520
BseMI GCAATG 2 cut(s) 814, 856
BseMII CTCAG 1 cut(s) 564
BseXI GCAGC 5 cut(s) 67, 196, 349, 517, 791
BseYI CCCAGC 1 cut(s) 150
BshFI GGCC 4 cut(s) 116, 280, 813, 881
BshVI ATCGAT 1 cut(s) 496
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 2 cut(s) 107, 583
BslFI GGGAC 3 cut(s) 186, 352, 413
BslI CCNNNNNNNGG 1 cut(s) 520
BsmAI GTCTC 3 cut(s) 352, 420, 747
BsmFI GGGAC 3 cut(s) 186, 352, 413
BsnI GGCC 4 cut(s) 116, 280, 813, 881
BsoBI CYCGRG 1 cut(s) 440
Bsp143I GATC 6 cut(s) 142, 436, 493, 646, 705, 730
Bsp1720I GCTNAGC 1 cut(s) 959
BspACI CCGC 4 cut(s) 38, 110, 158, 451
BspANI GGCC 4 cut(s) 116, 280, 813, 881
BspCNI CTCAG 1 cut(s) 563
BspDI ATCGAT 1 cut(s) 496
BspLI GGNNCC 2 cut(s) 202, 476
BspMAI CTGCAG 1 cut(s) 887
BspPI GGATC 1 cut(s) 654
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 814, 856
BsrFI RCCGGY 1 cut(s) 106
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 2 cut(s) 204, 282
BssMI GATC 6 cut(s) 142, 436, 493, 646, 705, 730
BssNAI GTATAC 1 cut(s) 1009
BssNI GRCGYC 1 cut(s) 389
BssT1I CCWWGG 1 cut(s) 282
Bst1107I GTATAC 1 cut(s) 1009
Bst2UI CCWGG 3 cut(s) 198, 515, 688
Bst4CI ACNGT 2 cut(s) 95, 779
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 389
BstAPI GCANNNNNTGC 1 cut(s) 882
BstC8I GCNNGC 3 cut(s) 108, 883, 905
BstDEI CTNAG 4 cut(s) 259, 550, 959, 987
BstDSI CCRYGG 1 cut(s) 204
BstENI CCTNNNNNAGG 1 cut(s) 518
BstF5I GGATG 3 cut(s) 154, 436, 773
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 6 cut(s) 145, 439, 496, 649, 708, 733
BstMAI GTCTC 3 cut(s) 352, 420, 747
BstMBI GATC 6 cut(s) 142, 436, 493, 646, 705, 730
BstMWI GCNNNNNNNGC 5 cut(s) 286, 337, 810, 846, 882
BstNI CCWGG 3 cut(s) 198, 515, 688
BstNSI RCATGY 1 cut(s) 907
BstSCI CCNGG 4 cut(s) 196, 513, 582, 686
BstSFI CTRYAG 2 cut(s) 393, 883
BstV1I GCAGC 5 cut(s) 67, 196, 349, 517, 791
BstV2I GAAGAC 1 cut(s) 312
BstZ17I GTATAC 1 cut(s) 1009
Bsu15I ATCGAT 1 cut(s) 496
BsuRI GGCC 4 cut(s) 116, 280, 813, 881
BsuTUI ATCGAT 1 cut(s) 496
BtgI CCRYGG 1 cut(s) 204
BtrI CACGTC 1 cut(s) 873
BtsCI GGATG 3 cut(s) 154, 436, 773
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 2 cut(s) 229, 784
Cac8I GCNNGC 3 cut(s) 108, 883, 905
CfoI GCGC 1 cut(s) 196
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 3 cut(s) 200, 279, 523
ClaI ATCGAT 1 cut(s) 496
CseI GACGC 1 cut(s) 378
Csp6I GTAC 1 cut(s) 569
CviAII CATG 6 cut(s) 179, 385, 680, 703, 904, 1000
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 4 cut(s) 259, 550, 959, 987
DpnI GATC 6 cut(s) 144, 438, 495, 648, 707, 732
DpnII GATC 6 cut(s) 142, 436, 493, 646, 705, 730
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 2 cut(s) 27, 147
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 881
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 2 cut(s) 200, 523
Eco88I CYCGRG 1 cut(s) 440
EcoNI CCTNNNNNAGG 1 cut(s) 518
EcoRII CCWGG 3 cut(s) 196, 513, 686
EcoRV GATATC 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 6 cut(s) 182, 388, 683, 706, 907, 1003
FaqI GGGAC 3 cut(s) 186, 352, 413
FatI CATG 6 cut(s) 178, 384, 679, 702, 903, 999
FbaI TGATCA 2 cut(s) 705, 730
FblI GTMKAC 2 cut(s) 392, 1008
Fnu4HI GCNGC 6 cut(s) 56, 210, 338, 452, 531, 805
FokI GGATG 3 cut(s) 141, 443, 780
Fsp4HI GCNGC 6 cut(s) 56, 210, 338, 452, 531, 805
GlaI GCGC 1 cut(s) 195
GluI GCNGC 6 cut(s) 56, 210, 338, 452, 531, 805
GsaI CCCAGC 1 cut(s) 154
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 4 cut(s) 116, 280, 813, 881
HapII CCGG 2 cut(s) 107, 583
HgaI GACGC 1 cut(s) 378
HhaI GCGC 1 cut(s) 196
Hin1I GRCGYC 1 cut(s) 389
Hin1II CATG 6 cut(s) 182, 388, 683, 706, 907, 1003
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HindIII AAGCTT 1 cut(s) 956
HinfI GANTC 4 cut(s) 125, 263, 401, 655
HpaII CCGG 2 cut(s) 107, 583
HphI GGTGA 1 cut(s) 785
Hpy166II GTNNAC 2 cut(s) 393, 1009
Hpy188I TCNGA 2 cut(s) 74, 99
Hpy188III TCNNGA 6 cut(s) 440, 442, 574, 604, 659, 728
Hpy8I GTNNAC 2 cut(s) 393, 1009
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 1 cut(s) 758
HpyCH4III ACNGT 2 cut(s) 95, 779
HpyCH4IV ACGT 1 cut(s) 872
HpyCH4V TGCA 6 cut(s) 212, 331, 807, 876, 885, 903
HpyF10VI GCNNNNNNNGC 5 cut(s) 286, 337, 810, 846, 882
HpyF3I CTNAG 4 cut(s) 259, 550, 959, 987
HpySE526I ACGT 1 cut(s) 872
Hsp92I GRCGYC 1 cut(s) 389
Hsp92II CATG 6 cut(s) 182, 388, 683, 706, 907, 1003
HspAI GCGC 1 cut(s) 194
KroI GCCGGC 1 cut(s) 106
KroNI GCCGGC 1 cut(s) 108
Ksp22I TGATCA 2 cut(s) 705, 730
Kzo9I GATC 6 cut(s) 142, 436, 493, 646, 705, 730
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 286, 585, 628
Lsp1109I GCAGC 5 cut(s) 67, 196, 349, 517, 791
LweI GCATC 3 cut(s) 163, 618, 934
MaeII ACGT 1 cut(s) 872
MaeIII GTNAC 2 cut(s) 224, 773
MalI GATC 6 cut(s) 144, 438, 495, 648, 707, 732
MboI GATC 6 cut(s) 142, 436, 493, 646, 705, 730
MboII GAAGA 4 cut(s) 312, 572, 631, 647
MlsI TGGCCA 1 cut(s) 813
MluCI AATT 3 cut(s) 76, 738, 823
MluNI TGGCCA 1 cut(s) 813
MlyI GAGTC 3 cut(s) 119, 395, 664
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 5 cut(s) 264, 421, 488, 856, 994
Mox20I TGGCCA 1 cut(s) 813
MreI CGCCGGCG 1 cut(s) 106
MroNI GCCGGC 1 cut(s) 106
MscI TGGCCA 1 cut(s) 813
MseI TTAA 3 cut(s) 723, 828, 1015
Msp20I TGGCCA 1 cut(s) 813
MspA1I CMGCKG 1 cut(s) 451
MspI CCGG 2 cut(s) 107, 583
MspR9I CCNGG 4 cut(s) 198, 515, 584, 688
MvaI CCWGG 3 cut(s) 198, 515, 688
MwoI GCNNNNNNNGC 5 cut(s) 286, 337, 810, 846, 882
NaeI GCCGGC 1 cut(s) 108
NciI CCSGG 1 cut(s) 584
NdeII GATC 6 cut(s) 142, 436, 493, 646, 705, 730
NgoMIV GCCGGC 1 cut(s) 106
NlaIII CATG 6 cut(s) 182, 388, 683, 706, 907, 1003
NlaIV GGNNCC 2 cut(s) 202, 476
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 2 cut(s) 224, 773
NspI RCATGY 1 cut(s) 907
PaeI GCATGC 1 cut(s) 907
PaeR7I CTCGAG 1 cut(s) 440
PceI AGGCCT 1 cut(s) 881
PciSI GCTCTTC 1 cut(s) 12
PdiI GCCGGC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 263
PflFI GACNNNGTC 1 cut(s) 521
PfoI TCCNGGA 2 cut(s) 513, 582
PkrI GCNGC 6 cut(s) 57, 211, 339, 453, 532, 806
PleI GAGTC 3 cut(s) 119, 395, 663
PpsI GAGTC 3 cut(s) 119, 395, 663
Psp6I CCWGG 3 cut(s) 196, 513, 686
PspFI CCCAGC 1 cut(s) 150
PspGI CCWGG 3 cut(s) 196, 513, 686
PspN4I GGNNCC 2 cut(s) 202, 476
PspPI GGNCC 3 cut(s) 200, 279, 523
PstI CTGCAG 1 cut(s) 887
PsyI GACNNNGTC 1 cut(s) 521
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 723, 828, 1015
SatI GCNGC 6 cut(s) 56, 210, 338, 452, 531, 805
Sau3AI GATC 6 cut(s) 142, 436, 493, 646, 705, 730
Sau96I GGNCC 3 cut(s) 200, 279, 523
ScaI AGTACT 1 cut(s) 570
SchI GAGTC 3 cut(s) 119, 395, 664
ScrFI CCNGG 4 cut(s) 198, 515, 584, 688
SfaNI GCATC 3 cut(s) 163, 618, 934
SfcI CTRYAG 2 cut(s) 393, 883
Sfr274I CTCGAG 1 cut(s) 440
SgrAI CRCCGGYG 1 cut(s) 106
SinI GGWCC 2 cut(s) 200, 523
SlaI CTCGAG 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 440, 716
SmoI CTYRAG 2 cut(s) 440, 716
SphI GCATGC 1 cut(s) 907
Sse9I AATT 3 cut(s) 76, 738, 823
SseBI AGGCCT 1 cut(s) 881
SsiI CCGC 4 cut(s) 38, 110, 158, 451
StuI AGGCCT 1 cut(s) 881
StyD4I CCNGG 4 cut(s) 196, 513, 582, 686
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 95, 779
TaiI ACGT 1 cut(s) 875
TaqI TCGA 5 cut(s) 90, 123, 441, 496, 603
TasI AATT 3 cut(s) 76, 738, 823
TatI WGTACW 1 cut(s) 568
TauI GCSGC 1 cut(s) 454
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 3 cut(s) 723, 828, 1015
Tru9I TTAA 3 cut(s) 723, 828, 1015
TscAI CASTG 2 cut(s) 229, 784
TseFI GTSAC 2 cut(s) 224, 773
TseI GCWGC 5 cut(s) 55, 209, 337, 530, 804
Tsp45I GTSAC 2 cut(s) 224, 773
TspDTI ATGAA 4 cut(s) 167, 696, 711, 909
TspRI CASTG 2 cut(s) 229, 784
Tth111I GACNNNGTC 1 cut(s) 521
VpaK11BI GGWCC 2 cut(s) 200, 523
XagI CCTNNNNNAGG 1 cut(s) 518
XapI RAATTY 1 cut(s) 76
XceI RCATGY 1 cut(s) 907
XhoI CTCGAG 1 cut(s) 440
XmiI GTMKAC 2 cut(s) 392, 1008
ZrmI AGTACT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.