pycom06g10640

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003275
Physical Location & Seq
Reverse (-)
15918575 .. 15921684
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g10640.1

Sequence Viewer

Length: 2004 bp
ATGGGGACCTTGGGGAGTCCTAGTGAAGAGCGTGAGGATCATTTTTATGATACCCGAGAGGACCTCTCTTCGGTTTCGGATTGGGGTTCAGATTGCTGTGAGGACTTTAGTTCTGGTGCTTCAGGTTTCTTAAGGTATGAGGAGGTTTGGGCTAAGAACCTGGAAAGTGTTCATGAGCGCCGCCATAAGTTCCTGGAGTGGATGGGTGTAGATTTGGATTCGCATTCGACGATGACAGAAGACTCAGAAGATATCTTTGTTGACAGAATTAAAATTATTGATAGATATAGGGAAGATAGCGGGGTAGTCTTGAGAACTTCGGGATTGGAAGTGGCGTCTTGCTCCAGTCAGTCTACAGTGTCTTCTCTGTCAACTGAAGCTCGGGAGCCACTGGGAAATGGTGCTTTGGATGATACTCATTCCACATCTGCAATGAGGAATTTGAACAGAGGAATGAATGTCATTGTGAATGAATTAGGTCATGATGGAAGGCTTGGCAGATTAGGTCAAGCTGCGCTAAATCAATTGGTTAATGAGGAAGTTCATAGTGCAGCGGCACCATCTCCTTCGATTGAGCAATTATTACAGAGAGAAGTTAATAACGCTAGGTTCTTTCTTGATTCGAAGAAGAAAGTTAAAAGGCGATGGTTTAGGAAATTAGGATTTGGGATTTGCATTGCTGATAAACATGAATCTCCGTTGAATCCCATCAGTCTTAAATCTGCATCAGGTGTAGGGATGCGAAGAGTCCGAGTTCATCCAAGTAAAAAACGGACCAAGGAACTATCTTCACTATATGCTGGGCAAGACTTTTCGGCACATGAGGGTTCAATTTTGTCCATGAAGTTCAGTCCTGATGGGCAATACTTGGCCAGTGCCGGTGAAGATGGTATCGTGCGAGTGTGGAAGGTGATTGAGGATGGGAAATTCAACAAATTTGATATTGCTTCTGATCCTTCTTCTCTATACTTCAAGATGCATCCATTTTCCAAGTTAAACTCTCCAAATGTGAATAAAGAAAAACAGAATAAGGCGCAGCAATTGGGGCGATCATCAGAGTCAGCTTGTGTCATTTTCCCTCCAAAGGTATTTCGCTTATTGGAGAAGCCTCTTCATGAGTTCCGGGGACACACAGGGGAGGTCTTAGATCTCTCATGGTCAACGAAAGGGTTTCTGCTCTCATCCTCAGTTGATAAGACGGTCCGTCTATGGCAAGTGGGATGCAACAGATGCCTGAGAATTTTTTCTCACAGTAATTTTGTGACATGTGTTAATTTCAACCCTCTGGATGATAATTATTTCATCAGCGGTTCAATAGACGGAAAAGTTCGCATCTGGGAAATTGTTCGTTGTCTGGTTGTTGATTATATTGATGTTCGCGAGATAGTCACTGCTATATCTTATTGTCCTGACGGAAAGGGAGGAATTGTGGGTTTGATGACTGGAAATTGCTGCATATTTAACATCATAGATGACCATATGGAATTGGATGTTCCGATAAACATACAGGGCAAAAAGAAGTCACCTGGAAAAAGGATAACCGGCTTTCAGTTTTCTCCAAGCAACCCAAGCAAAGTTATGGTGACGTCTGCTGATTCAGCAGTCCGTGTAATTTCTGGGAGAGATATCGTCTGCAAATATAGGGGTCTTCGAAATGGAGGAAACCATACGTCCGCATCTTTTACCACTGACGGAAAGCACATTGTCTCAGCAAGCGATGATTCAAATGTACACATCTGGAACTTCAATAGCCAGGACATGACCTCTTCGCGATCGAGGAAAATATGGTCTTGTGAGAGTTTCGTGTCCCACAACTCCATCATTGCTATACCTTGGAATGGTGTAAACAGCTTGCCGGGAACACTTCCATCCCCCAGATTCATTGGGGATGTGCCACAAAGTTGCCTTGATTATCCACCAAAGCATCTCAACTTTGATGAGAAATTAGATCAGAAAATTCCTTGCCAAGGGGTACCCCCACTTGGCCCGAGGAGAAGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

668

Amino Acids

74.28

Weight (kDa)

7.57

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 247 - 305 1.9e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 265 - 319 1.8e-07 THOC3 beta-propeller domain
WD40 PF00400 270 - 302 1.7e-09 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 270 - 321 3.9e-08 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 272 - 310 1.1e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 368 - 448 7.3e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 368 - 496 5.7e-12 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 369 - 590 2.1e-32 WDR5 beta-propeller domain
WD40 PF00400 369 - 404 1.9e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 371 - 431 4e-07 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 372 - 492 5.2e-06 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 372 - 536 1.6e-11 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 374 - 453 1.7e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 377 - 598 2.2e-22 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 381 - 586 2.8e-09 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 393 - 495 4.8e-06 WDR90/POC16, second beta-propeller
WD40 PF00400 410 - 446 2.9e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 431 - 614 3.7e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 459 - 599 6.8e-07 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 523 - 613 4e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1589
Acc65I GGTACC 1 cut(s) 1972
AccB1I GGYRCC 2 cut(s) 556, 1972
AccI GTMKAC 1 cut(s) 353
AccII CGCG 2 cut(s) 1380, 1771
AciI CCGC 5 cut(s) 181, 300, 554, 1308, 1674
AclWI GGATC 2 cut(s) 45, 947
AcoI YGGCCR 1 cut(s) 870
AcsI RAATTY 5 cut(s) 439, 926, 935, 1239, 1956
AcuI CTGAAG 2 cut(s) 105, 396
AcyI GRCGYC 2 cut(s) 335, 1586
AfaI GTAC 2 cut(s) 1731, 1974
AfiI CCNNNNNNNGG 5 cut(s) 70, 1084, 1838, 1967, 1982
AflII CTTAAG 1 cut(s) 130
AflIII ACRYGT 1 cut(s) 1265
AgsI TTSAA 9 cut(s) 445, 703, 831, 931, 973, 1279, 1314, 1725, 1747
AhdI GACNNNNNGTC 1 cut(s) 1203
AjnI CCWGG 4 cut(s) 159, 192, 1525, 1752
AluBI AGCT 5 cut(s) 380, 512, 1064, 1851, 1998
AluI AGCT 5 cut(s) 380, 512, 1064, 1851, 1998
Alw26I GTCTC 1 cut(s) 1711
AlwI GGATC 2 cut(s) 45, 947
Ama87I CYCGRG 3 cut(s) 54, 381, 1987
AoxI GGCC 2 cut(s) 870, 1984
ApeKI GCWGC 4 cut(s) 512, 551, 1036, 1452
ApoI RAATTY 5 cut(s) 439, 926, 935, 1239, 1956
Asp700I GAANNNNTTC 3 cut(s) 168, 1243, 1344
Asp718I GGTACC 1 cut(s) 1972
AspLEI GCGC 3 cut(s) 180, 517, 1036
AspS9I GGNCC 5 cut(s) 6, 61, 774, 1201, 1985
AsuC2I CCSGG 2 cut(s) 1124, 1857
AsuHPI GGTGA 4 cut(s) 893, 922, 1515, 1594
AsuII TTCGAA 2 cut(s) 623, 1651
AvaI CYCGRG 3 cut(s) 54, 381, 1987
AvaII GGWCC 4 cut(s) 6, 61, 774, 1201
BalI TGGCCA 1 cut(s) 872
BanI GGYRCC 2 cut(s) 556, 1972
BbsI GAAGAC 3 cut(s) 246, 354, 1640
BbvI GCAGC 4 cut(s) 499, 563, 1048, 1439
BciT130I CCWGG 4 cut(s) 161, 194, 1527, 1754
BcnI CCSGG 2 cut(s) 1124, 1857
BcoDI GTCTC 1 cut(s) 1711
BfaI CTAG 2 cut(s) 21, 606
BfmI CTRYAG 1 cut(s) 354
BfoI RGCGCY 1 cut(s) 181
BfrI CTTAAG 1 cut(s) 130
BglII AGATCT 1 cut(s) 1147
BisI GCNGC 6 cut(s) 181, 513, 552, 555, 1037, 1453
BlsI GCNGC 6 cut(s) 182, 514, 553, 556, 1038, 1454
Bme1390I CCNGG 6 cut(s) 161, 194, 1124, 1527, 1754, 1857
Bme18I GGWCC 4 cut(s) 6, 61, 774, 1201
BmeRI GACNNNNNGTC 1 cut(s) 1203
BmeT110I CYCGRG 3 cut(s) 54, 381, 1987
BmgT120I GGNCC 5 cut(s) 6, 61, 774, 1201, 1985
BmiI GGNNCC 4 cut(s) 7, 387, 558, 1974
BmrFI CCNGG 6 cut(s) 161, 194, 1124, 1527, 1754, 1857
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 9 cut(s) 729, 734, 966, 988, 1211, 1220, 1341, 1685, 1933
BmuI ACTGGG 1 cut(s) 401
BpiI GAAGAC 3 cut(s) 246, 354, 1640
BplI GAGNNNNNCTC 4 cut(s) 48, 80, 50, 82
BpmI CTGGAG 2 cut(s) 215, 328
Bpu14I TTCGAA 2 cut(s) 623, 1651
BpuEI CTTGAG 1 cut(s) 331
BpuMI CCSGG 2 cut(s) 1124, 1857
BsaHI GRCGYC 2 cut(s) 335, 1586
BsaJI CCNNGG 6 cut(s) 9, 777, 1123, 1832, 1966, 1988
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 1413, 1443
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 1084, 1838, 1967, 1982
Bse118I RCCGGY 2 cut(s) 878, 1541
Bse1I ACTGG 4 cut(s) 345, 396, 873, 1447
Bse3DI GCAATG 3 cut(s) 438, 675, 1821
BseBI CCWGG 4 cut(s) 161, 194, 1527, 1754
BseDI CCNNGG 6 cut(s) 9, 777, 1123, 1832, 1966, 1988
BseLI CCNNNNNNNGG 5 cut(s) 70, 1084, 1838, 1967, 1982
BseMI GCAATG 3 cut(s) 438, 675, 1821
BseMII CTCAG 4 cut(s) 258, 1200, 1226, 1722
BseNI ACTGG 4 cut(s) 345, 396, 873, 1447
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 4 cut(s) 499, 563, 1048, 1439
BseYI CCCAGC 1 cut(s) 800
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 2 cut(s) 1380, 1771
Bsh1285I CGRYCG 1 cut(s) 1775
BshFI GGCC 2 cut(s) 872, 1986
BshNI GGYRCC 2 cut(s) 556, 1972
BsiEI CGRYCG 1 cut(s) 1775
BsiHKCI CYCGRG 3 cut(s) 54, 381, 1987
BsiSI CCGG 4 cut(s) 879, 1123, 1542, 1856
BslFI GGGAC 3 cut(s) 19, 1140, 1792
BslI CCNNNNNNNGG 5 cut(s) 70, 1084, 1838, 1967, 1982
BsmAI GTCTC 1 cut(s) 1711
BsmFI GGGAC 3 cut(s) 19, 1140, 1792
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 2 cut(s) 872, 1986
BsoBI CYCGRG 3 cut(s) 54, 381, 1987
Bsp119I TTCGAA 2 cut(s) 623, 1651
Bsp1407I TGTACA 1 cut(s) 1729
Bsp143I GATC 6 cut(s) 37, 952, 1049, 1147, 1772, 1948
Bsp68I TCGCGA 2 cut(s) 1380, 1771
BspACI CCGC 5 cut(s) 181, 300, 554, 1308, 1674
BspANI GGCC 2 cut(s) 872, 1986
BspCNI CTCAG 4 cut(s) 257, 1199, 1227, 1721
BspFNI CGCG 2 cut(s) 1380, 1771
BspHI TCATGA 3 cut(s) 172, 481, 1114
BspLI GGNNCC 4 cut(s) 7, 387, 558, 1974
BspPI GGATC 2 cut(s) 45, 947
BspQI GCTCTTC 1 cut(s) 21
BspT104I TTCGAA 2 cut(s) 623, 1651
BspT107I GGYRCC 2 cut(s) 556, 1972
BspTI CTTAAG 1 cut(s) 130
BsrDI GCAATG 3 cut(s) 438, 675, 1821
BsrFI RCCGGY 2 cut(s) 878, 1541
BsrGI TGTACA 1 cut(s) 1729
BsrI ACTGG 4 cut(s) 345, 396, 873, 1447
BssAI RCCGGY 2 cut(s) 878, 1541
BssECI CCNNGG 6 cut(s) 9, 777, 1123, 1832, 1966, 1988
BssMI GATC 6 cut(s) 37, 952, 1049, 1147, 1772, 1948
BssNI GRCGYC 2 cut(s) 335, 1586
BssT1I CCWWGG 4 cut(s) 9, 777, 1832, 1966
Bst2UI CCWGG 4 cut(s) 161, 194, 1527, 1754
Bst4CI ACNGT 3 cut(s) 358, 1201, 1253
Bst6I CTCTTC 5 cut(s) 21, 73, 739, 1116, 1771
BstACI GRCGYC 2 cut(s) 335, 1586
BstAFI CTTAAG 1 cut(s) 130
BstAPI GCANNNNNTGC 1 cut(s) 1230
BstAUI TGTACA 1 cut(s) 1729
BstBI TTCGAA 2 cut(s) 623, 1651
BstC8I GCNNGC 2 cut(s) 1714, 1853
BstDEI CTNAG 6 cut(s) 153, 244, 1144, 1186, 1235, 1708
BstENI CCTNNNNNAGG 1 cut(s) 1965
BstFNI CGCG 2 cut(s) 1380, 1771
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 3 cut(s) 180, 517, 1036
BstKTI GATC 6 cut(s) 40, 955, 1052, 1150, 1775, 1951
BstMAI GTCTC 1 cut(s) 1711
BstMBI GATC 6 cut(s) 37, 952, 1049, 1147, 1772, 1948
BstMCI CGRYCG 1 cut(s) 1775
BstMWI GCNNNNNNNGC 4 cut(s) 1045, 1230, 1569, 1598
BstNI CCWGG 4 cut(s) 161, 194, 1527, 1754
BstNSI RCATGY 1 cut(s) 1269
BstSCI CCNGG 6 cut(s) 159, 192, 1122, 1525, 1752, 1855
BstSFI CTRYAG 1 cut(s) 354
BstUI CGCG 2 cut(s) 1380, 1771
BstV1I GCAGC 4 cut(s) 499, 563, 1048, 1439
BstV2I GAAGAC 3 cut(s) 246, 354, 1640
BstX2I RGATCY 1 cut(s) 1147
BstYI RGATCY 1 cut(s) 1147
BsuRI GGCC 2 cut(s) 872, 1986
BtgZI GCGATG 2 cut(s) 658, 1731
BtsI GCAGTG 1 cut(s) 1389
BtsIMutI CAGTG 5 cut(s) 363, 389, 880, 1389, 1686
BtuMI TCGCGA 2 cut(s) 1380, 1771
Cac8I GCNNGC 2 cut(s) 1714, 1853
CciI TCATGA 3 cut(s) 172, 481, 1114
CfoI GCGC 3 cut(s) 180, 517, 1036
Cfr10I RCCGGY 2 cut(s) 878, 1541
Cfr13I GGNCC 5 cut(s) 6, 61, 774, 1201, 1985
CpoI CGGWCCG 1 cut(s) 1201
CseI GACGC 1 cut(s) 324
Csp6I GTAC 2 cut(s) 1730, 1973
CspI CGGWCCG 1 cut(s) 1201
CviAII CATG 9 cut(s) 173, 482, 689, 821, 841, 1115, 1155, 1266, 1759
CviQI GTAC 2 cut(s) 1730, 1973
DdeI CTNAG 6 cut(s) 153, 244, 1144, 1186, 1235, 1708
DpnI GATC 6 cut(s) 39, 954, 1051, 1149, 1774, 1950
DpnII GATC 6 cut(s) 37, 952, 1049, 1147, 1772, 1948
DriI GACNNNNNGTC 1 cut(s) 1203
EaeI YGGCCR 1 cut(s) 870
Eam1104I CTCTTC 5 cut(s) 21, 73, 739, 1116, 1771
Eam1105I GACNNNNNGTC 1 cut(s) 1203
EarI CTCTTC 5 cut(s) 21, 73, 739, 1116, 1771
Eco130I CCWWGG 4 cut(s) 9, 777, 1832, 1966
Eco32I GATATC 2 cut(s) 253, 1627
Eco47I GGWCC 4 cut(s) 6, 61, 774, 1201
Eco57I CTGAAG 2 cut(s) 105, 396
Eco88I CYCGRG 3 cut(s) 54, 381, 1987
EcoNI CCTNNNNNAGG 1 cut(s) 1965
EcoO109I RGGNCCY 2 cut(s) 6, 61
EcoRII CCWGG 4 cut(s) 159, 192, 1525, 1752
EcoRV GATATC 2 cut(s) 253, 1627
EcoT14I CCWWGG 4 cut(s) 9, 777, 1832, 1966
EcoT22I ATGCAT 1 cut(s) 981
ErhI CCWWGG 4 cut(s) 9, 777, 1832, 1966
FaeI CATG 9 cut(s) 176, 485, 692, 824, 844, 1118, 1158, 1269, 1762
FaqI GGGAC 3 cut(s) 19, 1140, 1792
FatI CATG 9 cut(s) 172, 481, 688, 820, 840, 1114, 1154, 1265, 1758
FauI CCCGC 1 cut(s) 293
FauNDI CATATG 1 cut(s) 1479
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 6 cut(s) 181, 513, 552, 555, 1037, 1453
Fsp4HI GCNGC 6 cut(s) 181, 513, 552, 555, 1037, 1453
FspBI CTAG 2 cut(s) 21, 606
GlaI GCGC 3 cut(s) 179, 516, 1035
GluI GCNGC 6 cut(s) 181, 513, 552, 555, 1037, 1453
GsaI CCCAGC 1 cut(s) 804
GsuI CTGGAG 2 cut(s) 215, 328
HaeII RGCGCY 1 cut(s) 181
HaeIII GGCC 2 cut(s) 872, 1986
HapII CCGG 4 cut(s) 879, 1123, 1542, 1856
HgaI GACGC 1 cut(s) 324
HhaI GCGC 3 cut(s) 180, 517, 1036
Hin1I GRCGYC 2 cut(s) 335, 1586
Hin1II CATG 9 cut(s) 176, 485, 692, 824, 844, 1118, 1158, 1269, 1762
Hin6I GCGC 3 cut(s) 178, 515, 1034
HinP1I GCGC 3 cut(s) 178, 515, 1034
HincII GTYRAC 3 cut(s) 262, 372, 1161
HindII GTYRAC 3 cut(s) 262, 372, 1161
HindIII AAGCTT 1 cut(s) 1996
HpaII CCGG 4 cut(s) 879, 1123, 1542, 1856
HphI GGTGA 4 cut(s) 893, 922, 1515, 1594
Hpy166II GTNNAC 6 cut(s) 262, 354, 372, 1161, 1732, 1846
Hpy188I TCNGA 8 cut(s) 79, 91, 247, 752, 952, 1057, 1497, 1953
Hpy8I GTNNAC 6 cut(s) 262, 354, 372, 1161, 1732, 1846
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 4 cut(s) 483, 576, 901, 966
HpyCH4III ACNGT 3 cut(s) 358, 1201, 1253
HpyCH4IV ACGT 2 cut(s) 1586, 1670
HpyCH4V TGCA 8 cut(s) 431, 551, 675, 725, 979, 1224, 1455, 1635
HpyF10VI GCNNNNNNNGC 4 cut(s) 1045, 1230, 1569, 1598
HpyF3I CTNAG 6 cut(s) 153, 244, 1144, 1186, 1235, 1708
HpySE526I ACGT 2 cut(s) 1586, 1670
Hsp92I GRCGYC 2 cut(s) 335, 1586
Hsp92II CATG 9 cut(s) 176, 485, 692, 824, 844, 1118, 1158, 1269, 1762
HspAI GCGC 3 cut(s) 178, 515, 1034
KpnI GGTACC 1 cut(s) 1976
Kzo9I GATC 6 cut(s) 37, 952, 1049, 1147, 1772, 1948
LguI GCTCTTC 1 cut(s) 21
LmnI GCTCC 2 cut(s) 347, 385
Lsp1109I GCAGC 4 cut(s) 499, 563, 1048, 1439
LweI GCATC 9 cut(s) 729, 734, 966, 988, 1211, 1220, 1341, 1685, 1933
MaeI CTAG 2 cut(s) 21, 606
MaeII ACGT 2 cut(s) 1586, 1670
MaeIII GTNAC 4 cut(s) 1261, 1387, 1521, 1582
MalI GATC 6 cut(s) 39, 954, 1051, 1149, 1774, 1950
MboI GATC 6 cut(s) 37, 952, 1049, 1147, 1772, 1948
MfeI CAATTG 2 cut(s) 524, 1040
MflI RGATCY 1 cut(s) 1147
MlsI TGGCCA 1 cut(s) 872
MluNI TGGCCA 1 cut(s) 872
MlyI GAGTC 4 cut(s) 25, 236, 756, 1067
Mox20I TGGCCA 1 cut(s) 872
Mph1103I ATGCAT 1 cut(s) 981
MroXI GAANNNNTTC 3 cut(s) 168, 1243, 1344
MscI TGGCCA 1 cut(s) 872
MseI TTAA 9 cut(s) 131, 270, 531, 597, 636, 717, 995, 1272, 1461
MslI CAYNNNNRTG 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 872
MspA1I CMGCKG 2 cut(s) 554, 1308
MspCI CTTAAG 1 cut(s) 130
MspI CCGG 4 cut(s) 879, 1123, 1542, 1856
MspR9I CCNGG 6 cut(s) 161, 194, 1124, 1527, 1754, 1857
MunI CAATTG 2 cut(s) 524, 1040
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 4 cut(s) 161, 194, 1527, 1754
MvnI CGCG 2 cut(s) 1380, 1771
MwoI GCNNNNNNNGC 4 cut(s) 1045, 1230, 1569, 1598
NciI CCSGG 2 cut(s) 1124, 1857
NdeI CATATG 1 cut(s) 1479
NdeII GATC 6 cut(s) 37, 952, 1049, 1147, 1772, 1948
NlaIII CATG 9 cut(s) 176, 485, 692, 824, 844, 1118, 1158, 1269, 1762
NlaIV GGNNCC 4 cut(s) 7, 387, 558, 1974
NmuCI GTSAC 4 cut(s) 1261, 1387, 1521, 1582
NruI TCGCGA 2 cut(s) 1380, 1771
NsiI ATGCAT 1 cut(s) 981
NspI RCATGY 1 cut(s) 1269
NspV TTCGAA 2 cut(s) 623, 1651
PagI TCATGA 3 cut(s) 172, 481, 1114
PciI ACATGT 1 cut(s) 1265
PciSI GCTCTTC 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PctI GAATGC 1 cut(s) 223
PdmI GAANNNNTTC 3 cut(s) 168, 1243, 1344
PfeI GAWTC 7 cut(s) 218, 620, 692, 703, 1595, 1721, 1878
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 6 cut(s) 182, 514, 553, 556, 1038, 1454
Ple19I CGATCG 1 cut(s) 1775
PleI GAGTC 4 cut(s) 24, 236, 755, 1066
PpsI GAGTC 4 cut(s) 24, 236, 755, 1066
PpuMI RGGWCCY 2 cut(s) 6, 61
PscI ACATGT 1 cut(s) 1265
Psp5II RGGWCCY 2 cut(s) 6, 61
Psp6I CCWGG 4 cut(s) 159, 192, 1525, 1752
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 4 cut(s) 159, 192, 1525, 1752
PspN4I GGNNCC 4 cut(s) 7, 387, 558, 1974
PspPI GGNCC 5 cut(s) 6, 61, 774, 1201, 1985
PspPPI RGGWCCY 2 cut(s) 6, 61
PsuI RGATCY 1 cut(s) 1147
PvuI CGATCG 1 cut(s) 1775
RruI TCGCGA 2 cut(s) 1380, 1771
RsaI GTAC 2 cut(s) 1731, 1974
RsaNI GTAC 2 cut(s) 1730, 1973
RseI CAYNNNNRTG 1 cut(s) 45
Rsr2I CGGWCCG 1 cut(s) 1201
RsrII CGGWCCG 1 cut(s) 1201
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 9 cut(s) 131, 270, 531, 597, 636, 717, 995, 1272, 1461
SatI GCNGC 6 cut(s) 181, 513, 552, 555, 1037, 1453
Sau3AI GATC 6 cut(s) 37, 952, 1049, 1147, 1772, 1948
Sau96I GGNCC 5 cut(s) 6, 61, 774, 1201, 1985
SchI GAGTC 4 cut(s) 25, 236, 756, 1067
ScrFI CCNGG 6 cut(s) 161, 194, 1124, 1527, 1754, 1857
SfaNI GCATC 9 cut(s) 729, 734, 966, 988, 1211, 1220, 1341, 1685, 1933
SfcI CTRYAG 1 cut(s) 354
SfuI TTCGAA 2 cut(s) 623, 1651
SinI GGWCC 4 cut(s) 6, 61, 774, 1201
SmiMI CAYNNNNRTG 1 cut(s) 45
SmlI CTYRAG 2 cut(s) 130, 310
SmoI CTYRAG 2 cut(s) 130, 310
SsiI CCGC 5 cut(s) 181, 300, 554, 1308, 1674
SspMI CTAG 2 cut(s) 21, 606
StyD4I CCNGG 6 cut(s) 159, 192, 1122, 1525, 1752, 1855
StyI CCWWGG 4 cut(s) 9, 777, 1832, 1966
TaaI ACNGT 3 cut(s) 358, 1201, 1253
TaiI ACGT 2 cut(s) 1589, 1673
TaqI TCGA 5 cut(s) 227, 569, 623, 1651, 1775
TatI WGTACW 1 cut(s) 1729
TauI GCSGC 2 cut(s) 183, 557
TfiI GAWTC 7 cut(s) 218, 620, 692, 703, 1595, 1721, 1878
Tru1I TTAA 9 cut(s) 131, 270, 531, 597, 636, 717, 995, 1272, 1461
Tru9I TTAA 9 cut(s) 131, 270, 531, 597, 636, 717, 995, 1272, 1461
TscAI CASTG 5 cut(s) 363, 396, 880, 1396, 1693
TseFI GTSAC 4 cut(s) 1261, 1387, 1521, 1582
TseI GCWGC 4 cut(s) 512, 551, 1036, 1452
Tsp45I GTSAC 4 cut(s) 1261, 1387, 1521, 1582
TspGWI ACGGA 7 cut(s) 687, 787, 1193, 1335, 1428, 1595, 1707
TspRI CASTG 5 cut(s) 363, 396, 880, 1396, 1693
Vha464I CTTAAG 1 cut(s) 130
VpaK11BI GGWCC 4 cut(s) 6, 61, 774, 1201
XagI CCTNNNNNAGG 1 cut(s) 1965
XapI RAATTY 5 cut(s) 439, 926, 935, 1239, 1956
XceI RCATGY 1 cut(s) 1269
XmiI GTMKAC 1 cut(s) 353
XmnI GAANNNNTTC 3 cut(s) 168, 1243, 1344
XspI CTAG 2 cut(s) 21, 606
ZraI GACGTC 1 cut(s) 1587
Zsp2I ATGCAT 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.