MD14G1135100.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003275
Physical Location & Seq
Reverse (-)
21382112 .. 21386520
4409 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1135100.v1.1.491

Sequence Viewer

Length: 2175 bp
ATGGGGAGTTATGGTGAAGAGCGTGAGGATCAATTTTATGATACCCGAGAGGACCTTTCTTCGGTTTCGGATTGGGGTTCCGATTGCTGTGAGGAGTTTAGTTCTGGTACTCCAGGTTTCTTAAGGTATGAGGAGGTGTGGGCTAACAACTTGGAAAGTGTTCATGAGCGCCGCCATAAGTTCCTGCAGTGGATGGGTGTAGATTTGGTTTCGAATTCGATGATGACAGAAGACTCAGAAGATGTCTTTGTTGACAGAATTAAAATTATTGATAGAATTAGGGAAGATAGTGGGGTAATGTTGAGAAGTTGGGGATTGGAAGAGGCGTCTTGCTCAAGTCAGTCAACAGTGTCTTCTTTGTCAACTGAAGCTCGGGAATCACTAGGAAATGGTGCTTTGGATGATACACGTTCCACACGTGCAATGAGGAATTTGAACGGCGGAATGACTGTCATTGTAAGTGAATTGGTTCAGGATGGAAGGCTTGGCAGATTAGGTCAAGCGGGGCTAAATCAATTGGTTAATGGGGAAATTCTTAGTGCACAGGCACCGTCTCCTTTGATTCAGCAATTATTGCAGAGAGAAGTTGATAACGCTAGGTTCTTTCTTGATGCGAAGAAGAAAGTTAAAAGGGGATGGTTAAGGAAATTAGGAAATGGGATTTGCGTTGCTGATAAACATGAGGCTGAGGCTGCGTTGAGCCCCATCAGTCTTAAATCTGCGTCAGGGGTAGGGATGCAAAGAGTCCAAGTTCATCCAAGTAAAAAGCGGACCAAGGAATTATCCTCACTGTATGCTGGGCAACACTTTTCGGCTCACGAGGGTTCAATTTTGTCCATGAAGTTCAGTCTTGATGGGCAATACTTGGCCAGTGCTGGTGAAGATGGTGTCGTGAGAGTGTGGAAGGTGATTGAGGATGAGAGATTCAACAAATTTGATATTGCTTCTGATCCTTCTTCTCTGTACTTCAAGATGCATCCATTTTCCAAGTTAAACTCTCCCAATGTGAATAAAGAAAAACAGAGTAAGGCGCAGCAATTGGGGGGATCATCAGAGTCAGCTTGTGTCGTTTTCCCTCCAAAGATATTTCGCTTATTGGAGCAGCCTGTTCATGAGTTCCGGGGACACAGAGGGGAGGTCTTGGATCTCTCATGGTCAAAGAAAGGGTTTCTGCTCTCATCCTCTGTTGATAAGACAGTCCGTTTATGGCAAGTAGGATGCGACAGATGCCTGAGAGTCTTTTCTCACAGTAATTTTGTGACATGTGTTAATTTCAACCCTCTGGATGATAATTATTTCATCAGTGGTTCAATAGACGGAAAAGTTCGCATTTGGGAAATTATTCGTTGTCTGGTTGTTGACTATATTGATGTTCGCGAGATAGTCACTGCTGTATCTTATCGTCCTGACGGAAAGGGAGGAATTGTGGGTTTGATGACTGGAAATTGCTGTGTCTATAACATCATAGATGACCATCTGGAATTGGATGCTCAGATAACCGTACACGGCAAAAAGAAGTCACCTGGAAAAAGGATAACTGGCTTTCAGTTTTCTCCAAGCAACCCAAGCAAAGTTATGGTGACGTCTGCTGATTCAGTAGTCCGTATAATTTCTGGGAAAGATATCGTCTGCAAATATAGGGGTCTTCGAAATGGAGGAAACCATATGTCCGCCTCTTTTACCTCAGACGGAAAGCACATTGTCTCAGCAAGCGATGATTCAAATGTACGCATCTGGAACTGCAATAGCCAGGACACGACATCTTCGCGATCGAGGAACATATGGTCTTGTGAGAGTTTCGTGTCCCACAACTCCATGATTGCTATACCTTGGAATGGTGTAAACAGCTTGCCGGGAACCCTTGCATCCCCCAGATTCATTGGGGATTTGCCTCGAAGCAGCCTTGATTATCCCTTAAAACATCTCAATTTGGATGAGAAAGTAGAACAGAAGTCCTTGCCAAGGGGTACCGCGACTTGGCCCGAGGAGAAACTTGTAAACTCAAGTCCAGTACCCGTTTCCCCAATGATGTGCAGATCCGAGTACAAGTTTTTGAAGAGTGCTTGCCAGAGTATGTCTGGCTCATCTCACATGTGGGGCCTTGTGATCGTCACCGCCAGCCTGGACGGACGGATTAAAACATACCACAATTACGGGTTACCTATTCGTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

725

Amino Acids

80.58

Weight (kDa)

8.05

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 234 - 309 9.7e-06 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 249 - 311 6.4e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 262 - 323 8.3e-08 THOC3 beta-propeller domain
WD40 PF00400 269 - 301 7.2e-08 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 269 - 321 3.3e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 362 - 588 2.2e-32 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 367 - 447 5.2e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 367 - 488 8.9e-12 Prp19 WD40 domain
WD40 PF00400 368 - 403 5e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 368 - 430 2.9e-07 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 369 - 536 2.6e-11 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 372 - 452 5.6e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 376 - 597 4.5e-23 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 380 - 585 3.4e-11 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 393 - 495 4.2e-06 WDR90/POC16, second beta-propeller
WD40 PF00400 409 - 445 2.7e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 430 - 592 1e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 458 - 600 1.6e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 522 - 612 6.3e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1586
Acc65I GGTACC 1 cut(s) 1967
AccB1I GGYRCC 2 cut(s) 547, 1967
AccII CGCG 3 cut(s) 1377, 1768, 1973
AciI CCGC 7 cut(s) 172, 441, 503, 769, 1671, 1971, 2115
AclWI GGATC 5 cut(s) 36, 944, 1054, 1152, 2031
AcoI YGGCCR 1 cut(s) 867
AcsI RAATTY 4 cut(s) 214, 430, 531, 932
AcuI CTGAAG 1 cut(s) 387
AcvI CACGTG 1 cut(s) 419
AcyI GRCGYC 2 cut(s) 326, 1583
AfaI GTAC 7 cut(s) 109, 965, 1503, 1728, 1969, 2013, 2045
AfiI CCNNNNNNNGG 4 cut(s) 61, 1835, 1962, 1977
AflII CTTAAG 1 cut(s) 121
AflIII ACRYGT 4 cut(s) 407, 416, 1262, 2091
AgsI TTSAA 8 cut(s) 436, 828, 928, 970, 1276, 1311, 1722, 2056
AjnI CCWGG 4 cut(s) 112, 1522, 1749, 2121
AluBI AGCT 3 cut(s) 371, 1061, 1848
AluI AGCT 3 cut(s) 371, 1061, 1848
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 2 cut(s) 558, 1708
Alw44I GTGCAC 1 cut(s) 540
AlwI GGATC 5 cut(s) 36, 944, 1054, 1152, 2031
Ama87I CYCGRG 3 cut(s) 45, 372, 1982
AoxI GGCC 3 cut(s) 867, 1979, 2098
ApaLI GTGCAC 1 cut(s) 540
ApeKI GCWGC 4 cut(s) 692, 1033, 1102, 1899
ApoI RAATTY 4 cut(s) 214, 430, 531, 932
Asp700I GAANNNNTTC 3 cut(s) 159, 468, 1341
Asp718I GGTACC 1 cut(s) 1967
AspLEI GCGC 2 cut(s) 171, 1033
AspS9I GGNCC 4 cut(s) 52, 771, 1980, 2098
AsuC2I CCSGG 2 cut(s) 1121, 1854
AsuHPI GGTGA 6 cut(s) 26, 890, 919, 1512, 1591, 2104
AsuII TTCGAA 2 cut(s) 212, 1648
AvaI CYCGRG 3 cut(s) 45, 372, 1982
AvaII GGWCC 2 cut(s) 52, 771
BaeGI GKGCMC 1 cut(s) 544
BaeI ACNNNNGTAYC 2 cut(s) 1377, 1410
BalI TGGCCA 1 cut(s) 869
BanI GGYRCC 2 cut(s) 547, 1967
BanII GRGCYC 1 cut(s) 704
BauI CACGAG 1 cut(s) 818
BbrPI CACGTG 1 cut(s) 419
BbsI GAAGAC 3 cut(s) 237, 345, 1637
Bbv12I GWGCWC 1 cut(s) 544
BbvCI CCTCAGC 1 cut(s) 687
BbvI GCAGC 4 cut(s) 679, 1045, 1114, 1911
BccI CCATC 7 cut(s) 187, 470, 630, 713, 848, 878, 1482
BceAI ACGGC 2 cut(s) 454, 1522
BciT130I CCWGG 4 cut(s) 114, 1524, 1751, 2123
BcnI CCSGG 2 cut(s) 1121, 1854
BcoDI GTCTC 2 cut(s) 558, 1708
BfaI CTAG 2 cut(s) 383, 597
BfmI CTRYAG 1 cut(s) 185
BfoI RGCGCY 1 cut(s) 172
BfrI CTTAAG 1 cut(s) 121
BisI GCNGC 5 cut(s) 172, 693, 1034, 1103, 1900
BlsI GCNGC 5 cut(s) 173, 694, 1035, 1104, 1901
Bme1390I CCNGG 6 cut(s) 114, 1121, 1524, 1751, 1854, 2123
Bme18I GGWCC 2 cut(s) 52, 771
BmeT110I CYCGRG 3 cut(s) 45, 372, 1982
BmgT120I GGNCC 4 cut(s) 52, 771, 1980, 2098
BmiI GGNNCC 5 cut(s) 79, 549, 1858, 1969, 2099
BmrFI CCNGG 6 cut(s) 114, 1121, 1524, 1751, 1854, 2123
BmsI GCATC 9 cut(s) 601, 726, 963, 985, 1208, 1217, 1477, 1740, 1874
BpiI GAAGAC 3 cut(s) 237, 345, 1637
BpmI CTGGAG 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 687
Bpu14I TTCGAA 2 cut(s) 212, 1648
BpuEI CTTGAG 2 cut(s) 319, 1987
BpuMI CCSGG 2 cut(s) 1121, 1854
BsaAI YACGTR 1 cut(s) 419
BsaBI GATNNNNATC 1 cut(s) 1473
BsaHI GRCGYC 2 cut(s) 326, 1583
BsaJI CCNNGG 5 cut(s) 774, 1120, 1829, 1961, 1983
BsaXI ACNNNNNCTCC 3 cut(s) 28, 1410, 1440
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 1835, 1962, 1977
Bse1I ACTGG 4 cut(s) 870, 1444, 1543, 2009
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 1473
BseBI CCWGG 4 cut(s) 114, 1524, 1751, 2123
BseDI CCNNGG 5 cut(s) 774, 1120, 1829, 1961, 1983
BseJI GATNNNNATC 1 cut(s) 1473
BseLI CCNNNNNNNGG 4 cut(s) 61, 1835, 1962, 1977
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 6 cut(s) 249, 678, 1223, 1505, 1698, 1719
BseNI ACTGG 4 cut(s) 870, 1444, 1543, 2009
BseRI GAGGAG 3 cut(s) 107, 146, 2000
BseSI GKGCMC 1 cut(s) 544
BseXI GCAGC 4 cut(s) 679, 1045, 1114, 1911
BseYI CCCAGC 1 cut(s) 797
BsgI GTGCAG 1 cut(s) 2053
Bsh1236I CGCG 3 cut(s) 1377, 1768, 1973
Bsh1285I CGRYCG 1 cut(s) 1772
BshFI GGCC 3 cut(s) 869, 1981, 2100
BshNI GGYRCC 2 cut(s) 547, 1967
BsiEI CGRYCG 1 cut(s) 1772
BsiHKAI GWGCWC 1 cut(s) 544
BsiHKCI CYCGRG 3 cut(s) 45, 372, 1982
BsiSI CCGG 2 cut(s) 1120, 1853
BslFI GGGAC 2 cut(s) 1137, 1789
BslI CCNNNNNNNGG 4 cut(s) 61, 1835, 1962, 1977
BsmAI GTCTC 2 cut(s) 558, 1708
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 2 cut(s) 1137, 1789
BsnI GGCC 3 cut(s) 869, 1981, 2100
BsoBI CYCGRG 3 cut(s) 45, 372, 1982
Bsp119I TTCGAA 2 cut(s) 212, 1648
Bsp1286I GDGCHC 2 cut(s) 544, 704
Bsp143I GATC 7 cut(s) 28, 949, 1046, 1144, 1769, 2036, 2106
Bsp68I TCGCGA 2 cut(s) 1377, 1768
BspACI CCGC 7 cut(s) 172, 441, 503, 769, 1671, 1971, 2115
BspANI GGCC 3 cut(s) 869, 1981, 2100
BspCNI CTCAG 6 cut(s) 248, 679, 1224, 1504, 1697, 1718
BspFNI CGCG 3 cut(s) 1377, 1768, 1973
BspHI TCATGA 2 cut(s) 163, 1111
BspLI GGNNCC 5 cut(s) 79, 549, 1858, 1969, 2099
BspMAI CTGCAG 1 cut(s) 189
BspPI GGATC 5 cut(s) 36, 944, 1054, 1152, 2031
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 2 cut(s) 212, 1648
BspT107I GGYRCC 2 cut(s) 547, 1967
BspTI CTTAAG 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 4 cut(s) 870, 1444, 1543, 2009
BssECI CCNNGG 5 cut(s) 774, 1120, 1829, 1961, 1983
BssMI GATC 7 cut(s) 28, 949, 1046, 1144, 1769, 2036, 2106
BssNI GRCGYC 2 cut(s) 326, 1583
BssSI CACGAG 1 cut(s) 818
BssT1I CCWWGG 3 cut(s) 774, 1829, 1961
Bst2BI CACGAG 1 cut(s) 818
Bst2UI CCWGG 4 cut(s) 114, 1524, 1751, 2123
Bst4CI ACNGT 7 cut(s) 349, 451, 552, 792, 1198, 1250, 1501
Bst6I CTCTTC 3 cut(s) 12, 315, 2051
BstACI GRCGYC 2 cut(s) 326, 1583
BstAFI CTTAAG 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 574
BstBAI YACGTR 1 cut(s) 419
BstBI TTCGAA 2 cut(s) 212, 1648
BstC8I GCNNGC 4 cut(s) 1711, 1850, 2065, 2119
BstDEI CTNAG 7 cut(s) 235, 536, 687, 1232, 1491, 1684, 1705
BstEII GGTNACC 1 cut(s) 2157
BstENI CCTNNNNNAGG 1 cut(s) 1960
BstFNI CGCG 3 cut(s) 1377, 1768, 1973
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 2 cut(s) 171, 1033
BstKTI GATC 7 cut(s) 31, 952, 1049, 1147, 1772, 2039, 2109
BstMAI GTCTC 2 cut(s) 558, 1708
BstMBI GATC 7 cut(s) 28, 949, 1046, 1144, 1769, 2036, 2106
BstMCI CGRYCG 1 cut(s) 1772
BstMWI GCNNNNNNNGC 4 cut(s) 574, 692, 1227, 1566
BstNI CCWGG 4 cut(s) 114, 1524, 1751, 2123
BstNSI RCATGY 2 cut(s) 1266, 2095
BstPI GGTNACC 1 cut(s) 2157
BstSCI CCNGG 6 cut(s) 112, 1119, 1522, 1749, 1852, 2121
BstSFI CTRYAG 1 cut(s) 185
BstSLI GKGCMC 1 cut(s) 544
BstUI CGCG 3 cut(s) 1377, 1768, 1973
BstV1I GCAGC 4 cut(s) 679, 1045, 1114, 1911
BstV2I GAAGAC 3 cut(s) 237, 345, 1637
BstX2I RGATCY 2 cut(s) 1144, 2036
BstYI RGATCY 2 cut(s) 1144, 2036
BsuRI GGCC 3 cut(s) 869, 1981, 2100
BtgZI GCGATG 1 cut(s) 1728
BtsI GCAGTG 2 cut(s) 194, 1386
BtsIMutI CAGTG 6 cut(s) 194, 354, 788, 877, 1309, 1386
BtuMI TCGCGA 2 cut(s) 1377, 1768
Cac8I GCNNGC 4 cut(s) 1711, 1850, 2065, 2119
CciI TCATGA 2 cut(s) 163, 1111
CfoI GCGC 2 cut(s) 171, 1033
Cfr13I GGNCC 4 cut(s) 52, 771, 1980, 2098
CseI GACGC 2 cut(s) 315, 711
Csp6I GTAC 7 cut(s) 108, 964, 1502, 1727, 1968, 2012, 2044
CviAII CATG 8 cut(s) 164, 680, 838, 1112, 1152, 1263, 1816, 2092
CviQI GTAC 7 cut(s) 108, 964, 1502, 1727, 1968, 2012, 2044
DdeI CTNAG 7 cut(s) 235, 536, 687, 1232, 1491, 1684, 1705
DpnI GATC 7 cut(s) 30, 951, 1048, 1146, 1771, 2038, 2108
DpnII GATC 7 cut(s) 28, 949, 1046, 1144, 1769, 2036, 2106
EaeI YGGCCR 1 cut(s) 867
Eam1104I CTCTTC 3 cut(s) 12, 315, 2051
EarI CTCTTC 3 cut(s) 12, 315, 2051
EciI GGCGGA 2 cut(s) 456, 1660
Eco130I CCWWGG 3 cut(s) 774, 1829, 1961
Eco24I GRGCYC 1 cut(s) 704
Eco32I GATATC 1 cut(s) 1624
Eco47I GGWCC 2 cut(s) 52, 771
Eco57I CTGAAG 1 cut(s) 387
Eco72I CACGTG 1 cut(s) 419
Eco88I CYCGRG 3 cut(s) 45, 372, 1982
Eco91I GGTNACC 1 cut(s) 2157
EcoNI CCTNNNNNAGG 1 cut(s) 1960
EcoO109I RGGNCCY 2 cut(s) 52, 2098
EcoO65I GGTNACC 1 cut(s) 2157
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 4 cut(s) 112, 1522, 1749, 2121
EcoRV GATATC 1 cut(s) 1624
EcoT14I CCWWGG 3 cut(s) 774, 1829, 1961
EcoT22I ATGCAT 1 cut(s) 978
EcoT38I GRGCYC 1 cut(s) 704
ErhI CCWWGG 3 cut(s) 774, 1829, 1961
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 8 cut(s) 167, 683, 841, 1115, 1155, 1266, 1819, 2095
FaqI GGGAC 2 cut(s) 1137, 1789
FatI CATG 8 cut(s) 163, 679, 837, 1111, 1151, 1262, 1815, 2091
FauI CCCGC 1 cut(s) 496
FauNDI CATATG 2 cut(s) 1665, 1781
Fnu4HI GCNGC 5 cut(s) 172, 693, 1034, 1103, 1900
FriOI GRGCYC 1 cut(s) 704
Fsp4HI GCNGC 5 cut(s) 172, 693, 1034, 1103, 1900
FspBI CTAG 2 cut(s) 383, 597
GlaI GCGC 2 cut(s) 170, 1032
GluI GCNGC 5 cut(s) 172, 693, 1034, 1103, 1900
GsaI CCCAGC 1 cut(s) 801
GsuI CTGGAG 1 cut(s) 96
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 3 cut(s) 869, 1981, 2100
HapII CCGG 2 cut(s) 1120, 1853
HgaI GACGC 2 cut(s) 315, 711
HhaI GCGC 2 cut(s) 171, 1033
Hin1I GRCGYC 2 cut(s) 326, 1583
Hin1II CATG 8 cut(s) 167, 683, 841, 1115, 1155, 1266, 1819, 2095
Hin6I GCGC 2 cut(s) 169, 1031
HinP1I GCGC 2 cut(s) 169, 1031
HincII GTYRAC 4 cut(s) 253, 345, 363, 1360
HindII GTYRAC 4 cut(s) 253, 345, 363, 1360
HpaII CCGG 2 cut(s) 1120, 1853
HphI GGTGA 6 cut(s) 26, 890, 919, 1512, 1591, 2104
Hpy166II GTNNAC 8 cut(s) 253, 345, 363, 542, 1360, 1504, 1843, 1999
Hpy188I TCNGA 8 cut(s) 70, 82, 238, 949, 1054, 1494, 1687, 2041
Hpy8I GTNNAC 8 cut(s) 253, 345, 363, 542, 1360, 1504, 1843, 1999
Hpy99I CGWCG 1 cut(s) 2172
HpyAV CCTTC 3 cut(s) 474, 898, 963
HpyCH4III ACNGT 7 cut(s) 349, 451, 552, 792, 1198, 1250, 1501
HpyCH4IV ACGT 3 cut(s) 409, 418, 1583
HpyF10VI GCNNNNNNNGC 4 cut(s) 574, 692, 1227, 1566
HpyF3I CTNAG 7 cut(s) 235, 536, 687, 1232, 1491, 1684, 1705
HpySE526I ACGT 3 cut(s) 409, 418, 1583
Hsp92I GRCGYC 2 cut(s) 326, 1583
Hsp92II CATG 8 cut(s) 167, 683, 841, 1115, 1155, 1266, 1819, 2095
HspAI GCGC 2 cut(s) 169, 1031
KpnI GGTACC 1 cut(s) 1971
Kzo9I GATC 7 cut(s) 28, 949, 1046, 1144, 1769, 2036, 2106
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 1099
Lsp1109I GCAGC 4 cut(s) 679, 1045, 1114, 1911
LweI GCATC 9 cut(s) 601, 726, 963, 985, 1208, 1217, 1477, 1740, 1874
MaeI CTAG 2 cut(s) 383, 597
MaeII ACGT 3 cut(s) 409, 418, 1583
MaeIII GTNAC 6 cut(s) 1258, 1384, 1518, 1579, 2110, 2157
MalI GATC 7 cut(s) 30, 951, 1048, 1146, 1771, 2038, 2108
MboI GATC 7 cut(s) 28, 949, 1046, 1144, 1769, 2036, 2106
MfeI CAATTG 2 cut(s) 515, 1037
MflI RGATCY 2 cut(s) 1144, 2036
MhlI GDGCHC 2 cut(s) 544, 704
MlsI TGGCCA 1 cut(s) 869
MluNI TGGCCA 1 cut(s) 869
MlyI GAGTC 4 cut(s) 227, 753, 1064, 1245
Mox20I TGGCCA 1 cut(s) 869
Mph1103I ATGCAT 1 cut(s) 978
MroXI GAANNNNTTC 3 cut(s) 159, 468, 1341
MscI TGGCCA 1 cut(s) 869
Msp20I TGGCCA 1 cut(s) 869
MspCI CTTAAG 1 cut(s) 121
MspI CCGG 2 cut(s) 1120, 1853
MspR9I CCNGG 6 cut(s) 114, 1121, 1524, 1751, 1854, 2123
MunI CAATTG 2 cut(s) 515, 1037
MvaI CCWGG 4 cut(s) 114, 1524, 1751, 2123
MvnI CGCG 3 cut(s) 1377, 1768, 1973
MwoI GCNNNNNNNGC 4 cut(s) 574, 692, 1227, 1566
NciI CCSGG 2 cut(s) 1121, 1854
NdeI CATATG 2 cut(s) 1665, 1781
NdeII GATC 7 cut(s) 28, 949, 1046, 1144, 1769, 2036, 2106
NlaIII CATG 8 cut(s) 167, 683, 841, 1115, 1155, 1266, 1819, 2095
NlaIV GGNNCC 5 cut(s) 79, 549, 1858, 1969, 2099
NmuCI GTSAC 5 cut(s) 1258, 1384, 1518, 1579, 2110
NruI TCGCGA 2 cut(s) 1377, 1768
NsiI ATGCAT 1 cut(s) 978
NspI RCATGY 2 cut(s) 1266, 2095
NspV TTCGAA 2 cut(s) 212, 1648
PagI TCATGA 2 cut(s) 163, 1111
PciI ACATGT 2 cut(s) 1262, 2091
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 415
PdmI GAANNNNTTC 3 cut(s) 159, 468, 1341
PfeI GAWTC 6 cut(s) 377, 562, 924, 1592, 1718, 1875
PkrI GCNGC 5 cut(s) 173, 694, 1035, 1104, 1901
Ple19I CGATCG 1 cut(s) 1772
PleI GAGTC 4 cut(s) 227, 752, 1063, 1244
PmaCI CACGTG 1 cut(s) 419
PmlI CACGTG 1 cut(s) 419
PpsI GAGTC 4 cut(s) 227, 752, 1063, 1244
Ppu21I YACGTR 1 cut(s) 419
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 2 cut(s) 1262, 2091
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 4 cut(s) 112, 1522, 1749, 2121
PspCI CACGTG 1 cut(s) 419
PspEI GGTNACC 1 cut(s) 2157
PspFI CCCAGC 1 cut(s) 797
PspGI CCWGG 4 cut(s) 112, 1522, 1749, 2121
PspN4I GGNNCC 5 cut(s) 79, 549, 1858, 1969, 2099
PspPI GGNCC 4 cut(s) 52, 771, 1980, 2098
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 189
PsuI RGATCY 2 cut(s) 1144, 2036
PvuI CGATCG 1 cut(s) 1772
RruI TCGCGA 2 cut(s) 1377, 1768
RsaI GTAC 7 cut(s) 109, 965, 1503, 1728, 1969, 2013, 2045
RsaNI GTAC 7 cut(s) 108, 964, 1502, 1727, 1968, 2012, 2044
SapI GCTCTTC 1 cut(s) 12
SatI GCNGC 5 cut(s) 172, 693, 1034, 1103, 1900
Sau3AI GATC 7 cut(s) 28, 949, 1046, 1144, 1769, 2036, 2106
Sau96I GGNCC 4 cut(s) 52, 771, 1980, 2098
SchI GAGTC 4 cut(s) 227, 753, 1064, 1245
ScrFI CCNGG 6 cut(s) 114, 1121, 1524, 1751, 1854, 2123
SduI GDGCHC 2 cut(s) 544, 704
SfaNI GCATC 9 cut(s) 601, 726, 963, 985, 1208, 1217, 1477, 1740, 1874
SfcI CTRYAG 1 cut(s) 185
SfuI TTCGAA 2 cut(s) 212, 1648
SinI GGWCC 2 cut(s) 52, 771
SmlI CTYRAG 3 cut(s) 121, 334, 2002
SmoI CTYRAG 3 cut(s) 121, 334, 2002
SsiI CCGC 7 cut(s) 172, 441, 503, 769, 1671, 1971, 2115
SspMI CTAG 2 cut(s) 383, 597
StyD4I CCNGG 6 cut(s) 112, 1119, 1522, 1749, 1852, 2121
StyI CCWWGG 3 cut(s) 774, 1829, 1961
TaaI ACNGT 7 cut(s) 349, 451, 552, 792, 1198, 1250, 1501
TaiI ACGT 3 cut(s) 412, 421, 1586
TaqI TCGA 5 cut(s) 212, 218, 1648, 1772, 1894
TatI WGTACW 2 cut(s) 963, 2043
TauI GCSGC 1 cut(s) 174
TfiI GAWTC 6 cut(s) 377, 562, 924, 1592, 1718, 1875
TscAI CASTG 6 cut(s) 194, 354, 795, 877, 1309, 1393
TseFI GTSAC 5 cut(s) 1258, 1384, 1518, 1579, 2110
TseI GCWGC 4 cut(s) 692, 1033, 1102, 1899
Tsp45I GTSAC 5 cut(s) 1258, 1384, 1518, 1579, 2110
TspDTI ATGAA 6 cut(s) 152, 743, 854, 1100, 1288, 1867
TspGWI ACGGA 7 cut(s) 1190, 1332, 1425, 1592, 1704, 2142, 2146
TspRI CASTG 6 cut(s) 194, 354, 795, 877, 1309, 1393
Vha464I CTTAAG 1 cut(s) 121
VneI GTGCAC 1 cut(s) 540
VpaK11BI GGWCC 2 cut(s) 52, 771
XagI CCTNNNNNAGG 1 cut(s) 1960
XapI RAATTY 4 cut(s) 214, 430, 531, 932
XceI RCATGY 2 cut(s) 1266, 2095
XcmI CCANNNNNNNNNTGG 1 cut(s) 2075
XmnI GAANNNNTTC 3 cut(s) 159, 468, 1341
XspI CTAG 2 cut(s) 383, 597
ZraI GACGTC 1 cut(s) 1584
Zsp2I ATGCAT 1 cut(s) 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.