MD06G1113900.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003275
Physical Location & Seq
Reverse (-)
25201291 .. 25205813
4523 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1113900.v1.1.491

Sequence Viewer

Length: 2232 bp
ATGGGGACCTTGGGGAGTCCTAGTGAAGAGCGTGAGGATCATTTTTATGATACCCGAGAGGACCTCTCTTCGGTTTCGGATTGGGGTTCAGATTGCTGTGAGGACTTTAGTTCTGGTGCTTCAGGTTTCATAAGGTATGAGGAGGTTTGGGCTAAGAACCTGGAAAGTGTTCATGAGCGCCGCCATAAGTTCCTGGAGTGGATGGGTGTAGATTTGGATACGCATTCGACGATGACAGAAGACTCAGAAGATATCTTTGTTGACCGAATTAAAGTTATTGATAGATTTAGGGAAGATAGAGGGGTAGTCTTGAGAACTTCAGGATTGGAAGTGGCTTCTTGCTCAAGTCAGTCTACAGTGTCTTCTCTGTCAACTGAAGCTCGGGAGCCACTAGGAAATGGTGCTTTGGATGATACACATTCCACATCTGCAATGAGGAATTTGAACAGAGGAATGAATGTCATTGTGAATGAATTAGGTCATGATGGAAGGCTTGGCAGATTAGGTCAAGCTGCGTTAAATCAATTGGTTAATGGGGAAATTCATAGTGCACCGGCACCATCTCCTTCGATTCAGCAATTATTACAGAGAGAAGTTGATAACGCTAGGTTCTTTCTTGATACGAAGAAGAAAGTTAAAAGGGGATGGTTAAGGAAATTAAGAATTGGGATTTGTGTTGCTGATAAACATGAGGCTCCGTTGAGCCCCATCAGTCTTAAATCTGCATCAGGGGTAGGGATGCGAAGAGTCCGAGTTCATCCAAGTAAAAAGCAGACCAAGGAACTATCTTCACTATATGCCGGGCAAGACTTTTCGGCACATGAGGGTTCAATTTTGTCCATGAAGTTCAGTCCTGATGGGCAATACTTGGCCAGTTCTGGTGAAGATGGTATTGTGCGAGTGTGGAAGGTGATTGAGGATGAGAAATTCAACAAATTTGATATTGCTTCTGATCCTTCTTCTCTATACTTCAAGATGCATCCATTTTCCAAGTTAAATTCTCCAAATGTGAATAAAGAAAAACAGACTAAGGCGCAGCAATTGGGGCGATCATCAGAGTCAGCTTGTGTCATTTTCCCTCCAAAGGTATTTCGCTTATTGGAGAAGCCTATTCATGAGTTCCGGGGACACAGAGGGGAGGTCTTAGATCTCTCATGGTCAACGAAAGGGTTTCTGCTCTCATCCTCAGTTGATAAGACGGTCCGTCTATGGCAAGTGGGATGCGACAGATGCCTGAGAATTTTTTCTCACAGTAATTTTGTGACATGTGTTAATTTCAACCCTCTGGATGATAATTATTTCATCAGCGGTTCAATAGACGGAAAAGTTCGCATCTGGGAAATTGTTCGTTGTCTGGTTGTTGATTATATTGATGTTCGCGAGATAGTCACTGCTATATCTTATCGTCCTGACGGAAAGGGAGGAATTGTGGGTTTGATGACTGGAAATTGCTGCGTATTTAACATCATAGATGACCATATGGAATTGGATGTTCAGATAAACATACAGGGCAAAAAGAAGTCACCTGGAAAAAAGATAACTGGCTTTCAGTTTTCTCCAAGCAACCCAAGCAAAGTTATGGTGACGTCTGCTGATTCAGCAGTCCGTTTAATTTCTGGGGGAGATATCGTCTGCAAATATAGGGGTCTTCGAAATGGAGGAAACCATACATCTGCGTCCTTTACCACAGACGGAAAGCACATTGTCTCAGCAAGCGATGATTCAAATGTACACATCTGGAACTTCAATAGCCAGGACATGACCTCTTCGCGATTGAGGAAAATATGGTCTTGTGAGAGTTTCGTGTCCCACAACTCCATCATTGCTATACCTTGGAATGGTGTAAACAGCTTGCCGGGAACACTTCCATCCCCCAGATTCATTGGGGATGTGCCACAAAGTAGCATTGATTATCCACTAAAGCATCTCAACTTTGATGAGAAATTAGAACAGAAAATGCACCTCTCTTCGCCCGATTGCTTCTCTTTGGGTCGGGGGTTTTTGCTCGAGTCCTTGCCAAGGGGTACCCCCACTTGGCCCGAGGAGAAGCTTGTAAACTCAAGTCCAGTCCCCGTTTCCCCAACGATGTGTAGCTCCGAGTATAAGTTTTTGAAGAAAGCTTGCCAGAGCATGTCTGGTTCTTCTCACATGTGGGGCCTTGTAATTGTCACTGCCGGCTGGGATGGACGGATTAGAACCTACCACAATTACGGGTTACCTATTCGTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

744

Amino Acids

82.87

Weight (kDa)

8.06

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 242 - 311 6.6e-06 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 264 - 312 1.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 264 - 324 9.1e-08 THOC3 beta-propeller domain
WD40 PF00400 270 - 302 2.2e-09 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 270 - 324 6.2e-06 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 270 - 322 5.1e-09 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 272 - 306 2.1e-06 WDR5 beta-propeller domain
Beta-prop_WDR5 PF25175 369 - 589 9.5e-33 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 369 - 431 3.5e-07 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 369 - 498 1e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 369 - 448 1e-11 WDR3 first beta-propeller domain
WD40 PF00400 369 - 404 6.1e-07 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 370 - 536 1.2e-10 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 373 - 453 4e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 377 - 592 1.7e-22 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 381 - 587 1.2e-09 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 393 - 498 7.4e-06 WDR90/POC16, second beta-propeller
WD40 PF00400 410 - 446 4.1e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 431 - 594 2.7e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 459 - 590 2.1e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 517 - 613 8.7e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1589
Acc65I GGTACC 1 cut(s) 2024
AccB1I GGYRCC 2 cut(s) 556, 2024
AccI GTMKAC 1 cut(s) 353
AccII CGCG 2 cut(s) 1380, 1771
AciI CCGC 2 cut(s) 181, 1308
AclWI GGATC 2 cut(s) 45, 947
AcoI YGGCCR 1 cut(s) 870
AcsI RAATTY 6 cut(s) 439, 540, 926, 935, 997, 1239
AcuI CTGAAG 3 cut(s) 105, 303, 396
AcyI GRCGYC 1 cut(s) 1586
AfaI GTAC 2 cut(s) 1731, 2026
AfiI CCNNNNNNNGG 5 cut(s) 70, 1084, 1838, 2019, 2034
AflIII ACRYGT 2 cut(s) 1265, 2148
AgsI TTSAA 9 cut(s) 445, 831, 931, 973, 1279, 1314, 1725, 1747, 2113
AhdI GACNNNNNGTC 1 cut(s) 1203
AjnI CCWGG 4 cut(s) 159, 192, 1525, 1752
AluBI AGCT 7 cut(s) 380, 512, 1064, 1851, 2050, 2094, 2120
AluI AGCT 7 cut(s) 380, 512, 1064, 1851, 2050, 2094, 2120
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 1 cut(s) 1711
Alw44I GTGCAC 1 cut(s) 549
AlwI GGATC 2 cut(s) 45, 947
Ama87I CYCGRG 4 cut(s) 54, 381, 2006, 2039
AoxI GGCC 3 cut(s) 870, 2036, 2155
ApaLI GTGCAC 1 cut(s) 549
ApeKI GCWGC 3 cut(s) 512, 1036, 1452
ApoI RAATTY 6 cut(s) 439, 540, 926, 935, 997, 1239
Asp700I GAANNNNTTC 3 cut(s) 168, 1243, 1344
Asp718I GGTACC 1 cut(s) 2024
AspLEI GCGC 2 cut(s) 180, 1036
AspS9I GGNCC 5 cut(s) 6, 61, 1201, 2037, 2155
AsuC2I CCSGG 3 cut(s) 802, 1124, 1857
AsuHPI GGTGA 4 cut(s) 893, 922, 1515, 1594
AsuII TTCGAA 1 cut(s) 1651
AvaI CYCGRG 4 cut(s) 54, 381, 2006, 2039
AvaII GGWCC 3 cut(s) 6, 61, 1201
BaeGI GKGCMC 1 cut(s) 553
BalI TGGCCA 1 cut(s) 872
BanI GGYRCC 2 cut(s) 556, 2024
BanII GRGCYC 1 cut(s) 707
BbsI GAAGAC 3 cut(s) 246, 354, 1640
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 3 cut(s) 499, 1048, 1439
BciT130I CCWGG 4 cut(s) 161, 194, 1527, 1754
BciVI GTATCC 1 cut(s) 211
BcnI CCSGG 3 cut(s) 802, 1124, 1857
BcoDI GTCTC 1 cut(s) 1711
BfaI CTAG 3 cut(s) 21, 392, 606
BfmI CTRYAG 1 cut(s) 354
BfoI RGCGCY 1 cut(s) 181
BfuI GTATCC 1 cut(s) 211
BglII AGATCT 1 cut(s) 1147
BisI GCNGC 4 cut(s) 181, 513, 1037, 1453
BlsI GCNGC 4 cut(s) 182, 514, 1038, 1454
Bme1390I CCNGG 7 cut(s) 161, 194, 802, 1124, 1527, 1754, 1857
Bme18I GGWCC 3 cut(s) 6, 61, 1201
BmeRI GACNNNNNGTC 1 cut(s) 1203
BmeT110I CYCGRG 4 cut(s) 54, 381, 2006, 2039
BmgT120I GGNCC 5 cut(s) 6, 61, 1201, 2037, 2155
BmiI GGNNCC 6 cut(s) 7, 387, 558, 696, 2026, 2156
BmrFI CCNGG 7 cut(s) 161, 194, 802, 1124, 1527, 1754, 1857
BmsI GCATC 8 cut(s) 729, 734, 966, 988, 1211, 1220, 1341, 1933
BpiI GAAGAC 3 cut(s) 246, 354, 1640
BplI GAGNNNNNCTC 4 cut(s) 48, 80, 50, 82
BpmI CTGGAG 1 cut(s) 215
Bpu14I TTCGAA 1 cut(s) 1651
BpuEI CTTGAG 3 cut(s) 328, 331, 2044
BpuMI CCSGG 3 cut(s) 802, 1124, 1857
BsaHI GRCGYC 1 cut(s) 1586
BsaJI CCNNGG 6 cut(s) 9, 777, 1123, 1832, 2018, 2040
BsaXI ACNNNNNCTCC 6 cut(s) 7, 37, 1413, 1443, 1614, 1644
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 1084, 1838, 2019, 2034
Bse118I RCCGGY 2 cut(s) 553, 2174
Bse1I ACTGG 4 cut(s) 873, 1447, 1546, 2066
Bse3DI GCAATG 2 cut(s) 438, 1821
BseBI CCWGG 4 cut(s) 161, 194, 1527, 1754
BseDI CCNNGG 6 cut(s) 9, 777, 1123, 1832, 2018, 2040
BseLI CCNNNNNNNGG 5 cut(s) 70, 1084, 1838, 2019, 2034
BseMI GCAATG 2 cut(s) 438, 1821
BseMII CTCAG 4 cut(s) 258, 1200, 1226, 1722
BseNI ACTGG 4 cut(s) 873, 1447, 1546, 2066
BseRI GAGGAG 2 cut(s) 155, 2057
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 3 cut(s) 499, 1048, 1439
BseYI CCCAGC 1 cut(s) 2178
Bsh1236I CGCG 2 cut(s) 1380, 1771
BshFI GGCC 3 cut(s) 872, 2038, 2157
BshNI GGYRCC 2 cut(s) 556, 2024
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 4 cut(s) 54, 381, 2006, 2039
BsiSI CCGG 5 cut(s) 554, 801, 1123, 1856, 2175
BslFI GGGAC 4 cut(s) 19, 1140, 1792, 2054
BslI CCNNNNNNNGG 5 cut(s) 70, 1084, 1838, 2019, 2034
BsmAI GTCTC 1 cut(s) 1711
BsmFI GGGAC 4 cut(s) 19, 1140, 1792, 2054
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 3 cut(s) 872, 2038, 2157
BsoBI CYCGRG 4 cut(s) 54, 381, 2006, 2039
Bsp119I TTCGAA 1 cut(s) 1651
Bsp1286I GDGCHC 2 cut(s) 553, 707
Bsp1407I TGTACA 1 cut(s) 1729
Bsp143I GATC 4 cut(s) 37, 952, 1049, 1147
Bsp68I TCGCGA 2 cut(s) 1380, 1771
BspACI CCGC 2 cut(s) 181, 1308
BspANI GGCC 3 cut(s) 872, 2038, 2157
BspCNI CTCAG 4 cut(s) 257, 1199, 1227, 1721
BspFNI CGCG 2 cut(s) 1380, 1771
BspHI TCATGA 3 cut(s) 172, 481, 1114
BspLI GGNNCC 6 cut(s) 7, 387, 558, 696, 2026, 2156
BspPI GGATC 2 cut(s) 45, 947
BspQI GCTCTTC 1 cut(s) 21
BspT104I TTCGAA 1 cut(s) 1651
BspT107I GGYRCC 2 cut(s) 556, 2024
BsrDI GCAATG 2 cut(s) 438, 1821
BsrFI RCCGGY 2 cut(s) 553, 2174
BsrGI TGTACA 1 cut(s) 1729
BsrI ACTGG 4 cut(s) 873, 1447, 1546, 2066
BssAI RCCGGY 2 cut(s) 553, 2174
BssECI CCNNGG 6 cut(s) 9, 777, 1123, 1832, 2018, 2040
BssMI GATC 4 cut(s) 37, 952, 1049, 1147
BssNI GRCGYC 1 cut(s) 1586
BssT1I CCWWGG 4 cut(s) 9, 777, 1832, 2018
Bst2UI CCWGG 4 cut(s) 161, 194, 1527, 1754
Bst4CI ACNGT 3 cut(s) 358, 1201, 1253
Bst6I CTCTTC 5 cut(s) 21, 73, 739, 1771, 1972
BstACI GRCGYC 1 cut(s) 1586
BstAUI TGTACA 1 cut(s) 1729
BstBI TTCGAA 1 cut(s) 1651
BstC8I GCNNGC 4 cut(s) 1714, 1853, 2122, 2176
BstDEI CTNAG 7 cut(s) 153, 244, 1029, 1144, 1186, 1235, 1708
BstEII GGTNACC 1 cut(s) 2214
BstENI CCTNNNNNAGG 1 cut(s) 2017
BstFNI CGCG 2 cut(s) 1380, 1771
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 2 cut(s) 180, 1036
BstKTI GATC 4 cut(s) 40, 955, 1052, 1150
BstMAI GTCTC 1 cut(s) 1711
BstMBI GATC 4 cut(s) 37, 952, 1049, 1147
BstMWI GCNNNNNNNGC 4 cut(s) 1045, 1230, 1569, 1598
BstNI CCWGG 4 cut(s) 161, 194, 1527, 1754
BstNSI RCATGY 3 cut(s) 1269, 2134, 2152
BstPI GGTNACC 1 cut(s) 2214
BstSCI CCNGG 7 cut(s) 159, 192, 800, 1122, 1525, 1752, 1855
BstSFI CTRYAG 1 cut(s) 354
BstSLI GKGCMC 1 cut(s) 553
BstUI CGCG 2 cut(s) 1380, 1771
BstV1I GCAGC 3 cut(s) 499, 1048, 1439
BstV2I GAAGAC 3 cut(s) 246, 354, 1640
BstX2I RGATCY 1 cut(s) 1147
BstYI RGATCY 1 cut(s) 1147
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 3 cut(s) 872, 2038, 2157
BtgZI GCGATG 1 cut(s) 1731
BtsI GCAGTG 2 cut(s) 1389, 2169
BtsIMutI CAGTG 3 cut(s) 363, 1389, 2169
BtuMI TCGCGA 2 cut(s) 1380, 1771
Cac8I GCNNGC 4 cut(s) 1714, 1853, 2122, 2176
CciI TCATGA 3 cut(s) 172, 481, 1114
CfoI GCGC 2 cut(s) 180, 1036
Cfr10I RCCGGY 2 cut(s) 553, 2174
Cfr13I GGNCC 5 cut(s) 6, 61, 1201, 2037, 2155
CpoI CGGWCCG 1 cut(s) 1201
CseI GACGC 1 cut(s) 1665
Csp6I GTAC 2 cut(s) 1730, 2025
CspI CGGWCCG 1 cut(s) 1201
CviQI GTAC 2 cut(s) 1730, 2025
DdeI CTNAG 7 cut(s) 153, 244, 1029, 1144, 1186, 1235, 1708
DpnI GATC 4 cut(s) 39, 954, 1051, 1149
DpnII GATC 4 cut(s) 37, 952, 1049, 1147
DriI GACNNNNNGTC 1 cut(s) 1203
EaeI YGGCCR 1 cut(s) 870
Eam1104I CTCTTC 5 cut(s) 21, 73, 739, 1771, 1972
Eam1105I GACNNNNNGTC 1 cut(s) 1203
EarI CTCTTC 5 cut(s) 21, 73, 739, 1771, 1972
Eco130I CCWWGG 4 cut(s) 9, 777, 1832, 2018
Eco24I GRGCYC 1 cut(s) 707
Eco32I GATATC 2 cut(s) 253, 1627
Eco47I GGWCC 3 cut(s) 6, 61, 1201
Eco57I CTGAAG 3 cut(s) 105, 303, 396
Eco88I CYCGRG 4 cut(s) 54, 381, 2006, 2039
Eco91I GGTNACC 1 cut(s) 2214
EcoNI CCTNNNNNAGG 1 cut(s) 2017
EcoO109I RGGNCCY 3 cut(s) 6, 61, 2155
EcoO65I GGTNACC 1 cut(s) 2214
EcoRII CCWGG 4 cut(s) 159, 192, 1525, 1752
EcoRV GATATC 2 cut(s) 253, 1627
EcoT14I CCWWGG 4 cut(s) 9, 777, 1832, 2018
EcoT22I ATGCAT 1 cut(s) 981
EcoT38I GRGCYC 1 cut(s) 707
ErhI CCWWGG 4 cut(s) 9, 777, 1832, 2018
FaqI GGGAC 4 cut(s) 19, 1140, 1792, 2054
FauNDI CATATG 1 cut(s) 1479
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 4 cut(s) 181, 513, 1037, 1453
FriOI GRGCYC 1 cut(s) 707
Fsp4HI GCNGC 4 cut(s) 181, 513, 1037, 1453
FspBI CTAG 3 cut(s) 21, 392, 606
GlaI GCGC 2 cut(s) 179, 1035
GluI GCNGC 4 cut(s) 181, 513, 1037, 1453
GsaI CCCAGC 1 cut(s) 2182
GsuI CTGGAG 1 cut(s) 215
HaeII RGCGCY 1 cut(s) 181
HaeIII GGCC 3 cut(s) 872, 2038, 2157
HapII CCGG 5 cut(s) 554, 801, 1123, 1856, 2175
HgaI GACGC 1 cut(s) 1665
HhaI GCGC 2 cut(s) 180, 1036
Hin1I GRCGYC 1 cut(s) 1586
Hin6I GCGC 2 cut(s) 178, 1034
HinP1I GCGC 2 cut(s) 178, 1034
HincII GTYRAC 3 cut(s) 262, 372, 1161
HindII GTYRAC 3 cut(s) 262, 372, 1161
HindIII AAGCTT 2 cut(s) 2048, 2118
HinfI GANTC 9 cut(s) 16, 242, 571, 747, 1058, 1595, 1721, 1878, 2009
HpaII CCGG 5 cut(s) 554, 801, 1123, 1856, 2175
HphI GGTGA 4 cut(s) 893, 922, 1515, 1594
Hpy166II GTNNAC 8 cut(s) 262, 354, 372, 551, 1161, 1732, 1846, 2056
Hpy188I TCNGA 8 cut(s) 79, 91, 247, 752, 952, 1057, 1497, 2098
Hpy8I GTNNAC 8 cut(s) 262, 354, 372, 551, 1161, 1732, 1846, 2056
Hpy99I CGWCG 2 cut(s) 232, 2229
HpyAV CCTTC 4 cut(s) 483, 576, 901, 966
HpyCH4III ACNGT 3 cut(s) 358, 1201, 1253
HpyCH4IV ACGT 1 cut(s) 1586
HpyCH4V TGCA 6 cut(s) 431, 551, 725, 979, 1635, 1960
HpyF10VI GCNNNNNNNGC 4 cut(s) 1045, 1230, 1569, 1598
HpyF3I CTNAG 7 cut(s) 153, 244, 1029, 1144, 1186, 1235, 1708
HpySE526I ACGT 1 cut(s) 1586
Hsp92I GRCGYC 1 cut(s) 1586
HspAI GCGC 2 cut(s) 178, 1034
KpnI GGTACC 1 cut(s) 2028
KroI GCCGGC 1 cut(s) 2174
KroNI GCCGGC 1 cut(s) 2176
Kzo9I GATC 4 cut(s) 37, 952, 1049, 1147
LguI GCTCTTC 1 cut(s) 21
LmnI GCTCC 3 cut(s) 385, 700, 2099
Lsp1109I GCAGC 3 cut(s) 499, 1048, 1439
LweI GCATC 8 cut(s) 729, 734, 966, 988, 1211, 1220, 1341, 1933
MaeI CTAG 3 cut(s) 21, 392, 606
MaeII ACGT 1 cut(s) 1586
MaeIII GTNAC 6 cut(s) 1261, 1387, 1521, 1582, 2167, 2214
MalI GATC 4 cut(s) 39, 954, 1051, 1149
MboI GATC 4 cut(s) 37, 952, 1049, 1147
MfeI CAATTG 2 cut(s) 524, 1040
MflI RGATCY 1 cut(s) 1147
MhlI GDGCHC 2 cut(s) 553, 707
MlsI TGGCCA 1 cut(s) 872
MluNI TGGCCA 1 cut(s) 872
MlyI GAGTC 5 cut(s) 25, 236, 756, 1067, 2018
Mox20I TGGCCA 1 cut(s) 872
Mph1103I ATGCAT 1 cut(s) 981
MroNI GCCGGC 1 cut(s) 2174
MroXI GAANNNNTTC 3 cut(s) 168, 1243, 1344
MscI TGGCCA 1 cut(s) 872
MslI CAYNNNNRTG 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 872
MspA1I CMGCKG 1 cut(s) 1308
MspI CCGG 5 cut(s) 554, 801, 1123, 1856, 2175
MspR9I CCNGG 7 cut(s) 161, 194, 802, 1124, 1527, 1754, 1857
MunI CAATTG 2 cut(s) 524, 1040
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 4 cut(s) 161, 194, 1527, 1754
MvnI CGCG 2 cut(s) 1380, 1771
MwoI GCNNNNNNNGC 4 cut(s) 1045, 1230, 1569, 1598
NaeI GCCGGC 1 cut(s) 2176
NciI CCSGG 3 cut(s) 802, 1124, 1857
NdeI CATATG 1 cut(s) 1479
NdeII GATC 4 cut(s) 37, 952, 1049, 1147
NgoMIV GCCGGC 1 cut(s) 2174
NlaIV GGNNCC 6 cut(s) 7, 387, 558, 696, 2026, 2156
NmuCI GTSAC 5 cut(s) 1261, 1387, 1521, 1582, 2167
NruI TCGCGA 2 cut(s) 1380, 1771
NsiI ATGCAT 1 cut(s) 981
NspI RCATGY 3 cut(s) 1269, 2134, 2152
NspV TTCGAA 1 cut(s) 1651
PaeR7I CTCGAG 1 cut(s) 2006
PagI TCATGA 3 cut(s) 172, 481, 1114
PciI ACATGT 2 cut(s) 1265, 2148
PciSI GCTCTTC 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PctI GAATGC 1 cut(s) 223
PdiI GCCGGC 1 cut(s) 2176
PdmI GAANNNNTTC 3 cut(s) 168, 1243, 1344
PfeI GAWTC 4 cut(s) 571, 1595, 1721, 1878
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 4 cut(s) 182, 514, 1038, 1454
PleI GAGTC 5 cut(s) 24, 236, 755, 1066, 2017
PpsI GAGTC 5 cut(s) 24, 236, 755, 1066, 2017
PpuMI RGGWCCY 2 cut(s) 6, 61
PscI ACATGT 2 cut(s) 1265, 2148
Psp5II RGGWCCY 2 cut(s) 6, 61
Psp6I CCWGG 4 cut(s) 159, 192, 1525, 1752
PspEI GGTNACC 1 cut(s) 2214
PspFI CCCAGC 1 cut(s) 2178
PspGI CCWGG 4 cut(s) 159, 192, 1525, 1752
PspN4I GGNNCC 6 cut(s) 7, 387, 558, 696, 2026, 2156
PspPI GGNCC 5 cut(s) 6, 61, 1201, 2037, 2155
PspPPI RGGWCCY 2 cut(s) 6, 61
PspXI VCTCGAGB 1 cut(s) 2006
PsuI RGATCY 1 cut(s) 1147
RruI TCGCGA 2 cut(s) 1380, 1771
RsaI GTAC 2 cut(s) 1731, 2026
RsaNI GTAC 2 cut(s) 1730, 2025
RseI CAYNNNNRTG 1 cut(s) 45
Rsr2I CGGWCCG 1 cut(s) 1201
RsrII CGGWCCG 1 cut(s) 1201
SapI GCTCTTC 1 cut(s) 21
SatI GCNGC 4 cut(s) 181, 513, 1037, 1453
Sau3AI GATC 4 cut(s) 37, 952, 1049, 1147
Sau96I GGNCC 5 cut(s) 6, 61, 1201, 2037, 2155
SchI GAGTC 5 cut(s) 25, 236, 756, 1067, 2018
ScrFI CCNGG 7 cut(s) 161, 194, 802, 1124, 1527, 1754, 1857
SduI GDGCHC 2 cut(s) 553, 707
SfaNI GCATC 8 cut(s) 729, 734, 966, 988, 1211, 1220, 1341, 1933
SfcI CTRYAG 1 cut(s) 354
Sfr274I CTCGAG 1 cut(s) 2006
SfuI TTCGAA 1 cut(s) 1651
SinI GGWCC 3 cut(s) 6, 61, 1201
SlaI CTCGAG 1 cut(s) 2006
SmiMI CAYNNNNRTG 1 cut(s) 45
SmlI CTYRAG 4 cut(s) 310, 343, 2006, 2059
SmoI CTYRAG 4 cut(s) 310, 343, 2006, 2059
SsiI CCGC 2 cut(s) 181, 1308
SspMI CTAG 3 cut(s) 21, 392, 606
StyD4I CCNGG 7 cut(s) 159, 192, 800, 1122, 1525, 1752, 1855
StyI CCWWGG 4 cut(s) 9, 777, 1832, 2018
TaaI ACNGT 3 cut(s) 358, 1201, 1253
TaiI ACGT 1 cut(s) 1589
TaqI TCGA 4 cut(s) 227, 569, 1651, 2007
TaqII GACCGA 1 cut(s) 279
TatI WGTACW 1 cut(s) 1729
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 4 cut(s) 571, 1595, 1721, 1878
TscAI CASTG 3 cut(s) 363, 1396, 2176
TseFI GTSAC 5 cut(s) 1261, 1387, 1521, 1582, 2167
TseI GCWGC 3 cut(s) 512, 1036, 1452
Tsp45I GTSAC 5 cut(s) 1261, 1387, 1521, 1582, 2167
TspGWI ACGGA 7 cut(s) 687, 1193, 1335, 1428, 1595, 1707, 2203
TspRI CASTG 3 cut(s) 363, 1396, 2176
VneI GTGCAC 1 cut(s) 549
VpaK11BI GGWCC 3 cut(s) 6, 61, 1201
XagI CCTNNNNNAGG 1 cut(s) 2017
XapI RAATTY 6 cut(s) 439, 540, 926, 935, 997, 1239
XceI RCATGY 3 cut(s) 1269, 2134, 2152
XcmI CCANNNNNNNNNTGG 1 cut(s) 2132
XhoI CTCGAG 1 cut(s) 2006
XmiI GTMKAC 1 cut(s) 353
XmnI GAANNNNTTC 3 cut(s) 168, 1243, 1344
XspI CTAG 3 cut(s) 21, 392, 606
ZraI GACGTC 1 cut(s) 1587
Zsp2I ATGCAT 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.