FvH4_5g11810

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016847
Physical Location & Seq
Reverse (-)
6679486 .. 6683251
3766 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g11810.t1

Sequence Viewer

Length: 1281 bp
ATGAAGAACAAGCAGCATCAAAACCCTCCACCCTGTTTTCTCAAACACTTTGTGAATGCACAAATACAAATCAACAAGATCATCACCGTTTTCCTTATCTTCGTCTCCGGCCTTGCCCTCGGAATCACCCTAAGCTTCTACATCAAAGACACCCCTCTTCACATCTACACCGCATTAGCGCAAGCCAAAACCAGTTTAGATTCCACCGTATACAAATTAGTCCCTGTAGATAATTCTCCCCCACCACCACCCTCACCTGTTAATGAAACCAAAATCGCCACCGTTCCCCTCGCTTCACCGCCGCCTCGCCATCCATCTCCTCCCCAGCCGTCTCCTCCACCGCCGTCCAAAGCTGCTTACGTAAAGCCAAGTGCCATGCATGATATGGAAGATTCTGAGTTATTGTGGAGAGCTTCGATGGTGCCGAAAAGAAGTGGGGCATATCCATTCAAAAACGACACCAAGCCAAAAGTTGCCTTCATGTTTTTGACAAGAGGAGATTTGGCTCTGGCTCCTTTATGGGAACGTTTCTTCAAAGGACATGAAGGGTTGTACTCTATCTACATTCATGCCAATCCAGACTCAGGCGAGCCAATGCCGGAAAATTCTGTCTTTCACGGCAGAAGAGTCCCTAGTAAGAATGTGACTTGGGGCGAGCCAAACATGGTGCAAGCAGAGCGGCGCCTGTTAGCCAATGCACTACTGGACTTAACCAACCAACGTTTTATTCTTCTCTCCGAGTCGTGCATACCGCTCTTCAACTTCAAAACGGTCTATAATTACGTTATGGACTCAACCCAAACATTTGTGGAGGCCTACGACTTGCCCGGTGCCGTCGGTCGAGACCGATACAGGAAAAAAATGAGCCCGACAATCAAGCTTGAGCAATGGCGGAAGGGGTCGCAGTGGTTCGAGGTCGACCGCCAGTTGGCGCTGGGGGTGGTTTCAGACACTAGCTATTTTCCGTTGTTTGAGAAGTATTGCGTCCCTTCGTGCTACTCCGACGAGCACTACTTGCCTACTTATGTGAGCATTAGGTTTTGGAAGAAAAACTCTAACCGGACTTTGCATTGGGTCGACTGGTCGAGAGGAGGGCCACATCCGTCGAGGTATCAGAGGACGGATGTGACTTATGAGTTCTTGAACAAGATGAGATTTGGAACAACATGCGAGTACAATGGGAAGAGCACCAATATGTGCCATATGTTCGCGAGGAAGTTCATGCCAAACACATTAGATAGGCTGTTGAGGTTTGCTCCAAAGCTCATGCAGTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

427

Amino Acids

48.92

Weight (kDa)

9.5

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 158 - 385 1.7e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016847)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11810
malus_domestica MD14G1228400.v1.1 MD14G1228500.v1.1
prunus_persica Prupe.5G223500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0185791
rosa_laevigata RLG00000004883
rosa_multiflora Rmu_co8078508.1_g000001 Rmu_sc0002121.1_g000003
rosa_rugosa Rorug06G0471300
rosa_samantha Rh7AG071000 Rh7BG076800 Rh7CG076800 Rh7DG076700
rosa_wichuraiana Rw7G006400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 421, 681, 830
AccBSI CCGCTC 2 cut(s) 679, 754
AccI GTMKAC 3 cut(s) 210, 918, 1077
AccII CGCG 1 cut(s) 1211
AciI CCGC 8 cut(s) 171, 299, 302, 341, 679, 752, 892, 922
AclI AACGTT 2 cut(s) 526, 721
AcsI RAATTY 1 cut(s) 604
AcyI GRCGYC 1 cut(s) 682
AdeI CACNNNGTG 1 cut(s) 52
AfaI GTAC 2 cut(s) 554, 1175
AfiI CCNNNNNNNGG 2 cut(s) 584, 928
AgsI TTSAA 6 cut(s) 451, 535, 760, 766, 1144, 1276
AjuI GAANNNNNNNTTGG 2 cut(s) 711, 743
AluBI AGCT 6 cut(s) 135, 353, 413, 880, 957, 1264
AluI AGCT 6 cut(s) 135, 353, 413, 880, 957, 1264
Alw21I GWGCWC 2 cut(s) 1011, 1190
Alw26I GTCTC 3 cut(s) 109, 336, 837
AoxI GGCC 3 cut(s) 109, 813, 1094
ApeKI GCWGC 2 cut(s) 13, 353
ApoI RAATTY 1 cut(s) 604
AspLEI GCGC 3 cut(s) 181, 684, 934
AspS9I GGNCC 1 cut(s) 1094
AsuC2I CCSGG 1 cut(s) 828
AsuHPI GGTGA 4 cut(s) 76, 118, 246, 288
BanI GGYRCC 3 cut(s) 421, 681, 830
BanII GRGCYC 1 cut(s) 869
Bbv12I GWGCWC 2 cut(s) 1011, 1190
BbvI GCAGC 2 cut(s) 25, 340
BccI CCATC 3 cut(s) 318, 322, 412
BceAI ACGGC 4 cut(s) 313, 328, 634, 818
BcnI CCSGG 1 cut(s) 828
BcoDI GTCTC 3 cut(s) 109, 336, 837
BfaI CTAG 2 cut(s) 633, 954
BfmI CTRYAG 1 cut(s) 225
BfoI RGCGCY 2 cut(s) 685, 935
BisI GCNGC 4 cut(s) 14, 302, 354, 680
BlsI GCNGC 4 cut(s) 15, 303, 355, 681
Bme1390I CCNGG 1 cut(s) 828
BmgT120I GGNCC 1 cut(s) 1094
BmiI GGNNCC 4 cut(s) 423, 513, 683, 832
BmrFI CCNGG 1 cut(s) 828
BmsI GCATC 1 cut(s) 25
BplI GAGNNNNNCTC 2 cut(s) 1240, 1272
Bpu10I CCTNAGC 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 1 cut(s) 828
BsaAI YACGTR 1 cut(s) 361
BsaHI GRCGYC 1 cut(s) 682
BsaI GGTCTC 1 cut(s) 837
BsaJI CCNNGG 1 cut(s) 118
BsaWI WCCGGW 1 cut(s) 1059
Bsc4I CCNNNNNNNGG 2 cut(s) 584, 928
Bse1I ACTGG 4 cut(s) 192, 708, 925, 1085
Bse3DI GCAATG 1 cut(s) 893
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 3 cut(s) 310, 1099, 1129
BseLI CCNNNNNNNGG 2 cut(s) 584, 928
BseMI GCAATG 1 cut(s) 893
BseMII CTCAG 2 cut(s) 387, 597
BseNI ACTGG 4 cut(s) 192, 708, 925, 1085
BseRI GAGGAG 4 cut(s) 309, 324, 510, 1104
BseXI GCAGC 2 cut(s) 25, 340
BseYI CCCAGC 2 cut(s) 324, 934
Bsh1236I CGCG 1 cut(s) 1211
Bsh1285I CGRYCG 2 cut(s) 841, 922
BshFI GGCC 3 cut(s) 111, 815, 1096
BshNI GGYRCC 3 cut(s) 421, 681, 830
BsiEI CGRYCG 2 cut(s) 841, 922
BsiHKAI GWGCWC 2 cut(s) 1011, 1190
BsiSI CCGG 4 cut(s) 108, 599, 828, 1060
BslFI GGGAC 3 cut(s) 206, 614, 971
BslI CCNNNNNNNGG 2 cut(s) 584, 928
BsmAI GTCTC 3 cut(s) 109, 336, 837
BsmBI CGTCTC 2 cut(s) 109, 336
BsmFI GGGAC 3 cut(s) 206, 614, 971
BsmI GAATGC 1 cut(s) 61
BsnI GGCC 3 cut(s) 111, 815, 1096
Bso31I GGTCTC 1 cut(s) 837
Bsp1286I GDGCHC 3 cut(s) 869, 1011, 1190
Bsp143I GATC 1 cut(s) 78
Bsp68I TCGCGA 1 cut(s) 1211
BspACI CCGC 8 cut(s) 171, 299, 302, 341, 679, 752, 892, 922
BspANI GGCC 3 cut(s) 111, 815, 1096
BspCNI CTCAG 2 cut(s) 388, 596
BspFNI CGCG 1 cut(s) 1211
BspLI GGNNCC 4 cut(s) 423, 513, 683, 832
BspQI GCTCTTC 2 cut(s) 761, 1178
BspT107I GGYRCC 3 cut(s) 421, 681, 830
BspTNI GGTCTC 1 cut(s) 837
BsrBI CCGCTC 2 cut(s) 679, 754
BsrDI GCAATG 1 cut(s) 893
BsrI ACTGG 4 cut(s) 192, 708, 925, 1085
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 1 cut(s) 78
BssNAI GTATAC 1 cut(s) 211
BssNI GRCGYC 1 cut(s) 682
Bst1107I GTATAC 1 cut(s) 211
Bst4CI ACNGT 4 cut(s) 88, 208, 283, 772
Bst6I CTCTTC 4 cut(s) 162, 619, 761, 1178
BstACI GRCGYC 1 cut(s) 682
BstAPI GCANNNNNTGC 1 cut(s) 1015
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 4 cut(s) 183, 590, 656, 672
BstDEI CTNAG 3 cut(s) 131, 396, 583
BstF5I GGATG 3 cut(s) 310, 1099, 1129
BstFNI CGCG 1 cut(s) 1211
BstH2I RGCGCY 2 cut(s) 685, 935
BstHHI GCGC 3 cut(s) 181, 684, 934
BstKTI GATC 1 cut(s) 81
BstMAI GTCTC 3 cut(s) 109, 336, 837
BstMBI GATC 1 cut(s) 78
BstMCI CGRYCG 2 cut(s) 841, 922
BstMWI GCNNNNNNNGC 2 cut(s) 676, 1015
BstNSI RCATGY 1 cut(s) 1170
BstSCI CCNGG 1 cut(s) 826
BstSFI CTRYAG 1 cut(s) 225
BstSNI TACGTA 1 cut(s) 361
BstUI CGCG 1 cut(s) 1211
BstV1I GCAGC 2 cut(s) 25, 340
BstZ17I GTATAC 1 cut(s) 211
BsuRI GGCC 3 cut(s) 111, 815, 1096
BtsCI GGATG 3 cut(s) 310, 1099, 1129
BtsI GCAGTG 1 cut(s) 911
BtsIMutI CAGTG 1 cut(s) 911
BtuMI TCGCGA 1 cut(s) 1211
Cac8I GCNNGC 4 cut(s) 183, 590, 656, 672
CfoI GCGC 3 cut(s) 181, 684, 934
Cfr13I GGNCC 1 cut(s) 1094
CseI GACGC 1 cut(s) 973
Csp6I GTAC 2 cut(s) 553, 1174
CviAII CATG 9 cut(s) 376, 380, 481, 542, 569, 664, 1167, 1222, 1267
CviQI GTAC 2 cut(s) 553, 1174
DdeI CTNAG 3 cut(s) 131, 396, 583
DinI GGCGCC 1 cut(s) 683
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
DraIII CACNNNGTG 1 cut(s) 52
Eam1104I CTCTTC 4 cut(s) 162, 619, 761, 1178
EarI CTCTTC 4 cut(s) 162, 619, 761, 1178
EciI GGCGGA 1 cut(s) 907
Eco105I TACGTA 1 cut(s) 361
Eco147I AGGCCT 1 cut(s) 815
Eco24I GRGCYC 1 cut(s) 869
Eco31I GGTCTC 1 cut(s) 837
EcoT22I ATGCAT 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 869
EgeI GGCGCC 1 cut(s) 683
EheI GGCGCC 1 cut(s) 683
Esp3I CGTCTC 2 cut(s) 109, 336
FaeI CATG 9 cut(s) 379, 383, 484, 545, 572, 667, 1170, 1225, 1270
FaqI GGGAC 3 cut(s) 206, 614, 971
FatI CATG 9 cut(s) 375, 379, 480, 541, 568, 663, 1166, 1221, 1266
FauNDI CATATG 1 cut(s) 1203
FblI GTMKAC 3 cut(s) 210, 918, 1077
Fnu4HI GCNGC 4 cut(s) 14, 302, 354, 680
FokI GGATG 3 cut(s) 297, 1086, 1136
FriOI GRGCYC 1 cut(s) 869
Fsp4HI GCNGC 4 cut(s) 14, 302, 354, 680
FspBI CTAG 2 cut(s) 633, 954
GlaI GCGC 3 cut(s) 180, 683, 933
GluI GCNGC 4 cut(s) 14, 302, 354, 680
GsaI CCCAGC 2 cut(s) 328, 938
HaeII RGCGCY 2 cut(s) 685, 935
HaeIII GGCC 3 cut(s) 111, 815, 1096
HapII CCGG 4 cut(s) 108, 599, 828, 1060
HgaI GACGC 1 cut(s) 973
HhaI GCGC 3 cut(s) 181, 684, 934
Hin1I GRCGYC 1 cut(s) 682
Hin1II CATG 9 cut(s) 379, 383, 484, 545, 572, 667, 1170, 1225, 1270
Hin6I GCGC 3 cut(s) 179, 682, 932
HinP1I GCGC 3 cut(s) 179, 682, 932
HincII GTYRAC 2 cut(s) 919, 1078
HindII GTYRAC 2 cut(s) 919, 1078
HindIII AAGCTT 2 cut(s) 133, 878
HinfI GANTC 7 cut(s) 123, 200, 392, 581, 627, 740, 791
HpaII CCGG 4 cut(s) 108, 599, 828, 1060
HphI GGTGA 4 cut(s) 76, 118, 246, 288
Hpy166II GTNNAC 3 cut(s) 211, 919, 1078
Hpy188I TCNGA 6 cut(s) 122, 397, 739, 949, 1003, 1116
Hpy188III TCNNGA 5 cut(s) 578, 842, 1086, 1141, 1210
Hpy8I GTNNAC 3 cut(s) 211, 919, 1078
Hpy99I CGWCG 3 cut(s) 839, 1007, 1108
HpyAV CCTTC 4 cut(s) 487, 539, 889, 999
HpyCH4III ACNGT 4 cut(s) 88, 208, 283, 772
HpyCH4IV ACGT 4 cut(s) 360, 526, 721, 783
HpyCH4V TGCA 7 cut(s) 59, 379, 670, 698, 747, 1069, 1270
HpyF10VI GCNNNNNNNGC 2 cut(s) 676, 1015
HpyF3I CTNAG 3 cut(s) 131, 396, 583
HpySE526I ACGT 4 cut(s) 360, 526, 721, 783
Hsp92I GRCGYC 1 cut(s) 682
Hsp92II CATG 9 cut(s) 379, 383, 484, 545, 572, 667, 1170, 1225, 1270
HspAI GCGC 3 cut(s) 179, 682, 932
KasI GGCGCC 1 cut(s) 681
Kzo9I GATC 1 cut(s) 78
LguI GCTCTTC 2 cut(s) 761, 1178
LmnI GCTCC 2 cut(s) 517, 1261
Lsp1109I GCAGC 2 cut(s) 25, 340
LweI GCATC 1 cut(s) 25
MaeI CTAG 2 cut(s) 633, 954
MaeII ACGT 4 cut(s) 360, 526, 721, 783
MaeIII GTNAC 2 cut(s) 643, 1126
MalI GATC 1 cut(s) 80
MbiI CCGCTC 2 cut(s) 679, 754
MboI GATC 1 cut(s) 78
MfeI CAATTG 1 cut(s) 1276
MhlI GDGCHC 3 cut(s) 869, 1011, 1190
MluCI AATT 5 cut(s) 215, 232, 604, 778, 1276
Mly113I GGCGCC 1 cut(s) 682
MlyI GAGTC 4 cut(s) 575, 636, 749, 785
MmeI TCCRAC 1 cut(s) 1026
Mph1103I ATGCAT 1 cut(s) 381
MseI TTAA 2 cut(s) 261, 710
MslI CAYNNNNRTG 1 cut(s) 1193
MspI CCGG 4 cut(s) 108, 599, 828, 1060
MspR9I CCNGG 1 cut(s) 828
MunI CAATTG 1 cut(s) 1276
Mva1269I GAATGC 1 cut(s) 61
MvnI CGCG 1 cut(s) 1211
MwoI GCNNNNNNNGC 2 cut(s) 676, 1015
NarI GGCGCC 1 cut(s) 682
NciI CCSGG 1 cut(s) 828
NdeI CATATG 1 cut(s) 1203
NdeII GATC 1 cut(s) 78
NlaIII CATG 9 cut(s) 379, 383, 484, 545, 572, 667, 1170, 1225, 1270
NlaIV GGNNCC 4 cut(s) 423, 513, 683, 832
NmuCI GTSAC 2 cut(s) 643, 1126
NruI TCGCGA 1 cut(s) 1211
NsiI ATGCAT 1 cut(s) 381
NspI RCATGY 1 cut(s) 1170
PceI AGGCCT 1 cut(s) 815
PciSI GCTCTTC 2 cut(s) 761, 1178
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PctI GAATGC 1 cut(s) 61
PfeI GAWTC 3 cut(s) 123, 200, 392
PkrI GCNGC 4 cut(s) 15, 303, 355, 681
PleI GAGTC 4 cut(s) 575, 635, 748, 785
PluTI GGCGCC 1 cut(s) 685
PpsI GAGTC 4 cut(s) 575, 635, 748, 785
Ppu21I YACGTR 1 cut(s) 361
Psp1406I AACGTT 2 cut(s) 526, 721
PspFI CCCAGC 2 cut(s) 324, 934
PspN4I GGNNCC 4 cut(s) 423, 513, 683, 832
PspPI GGNCC 1 cut(s) 1094
RruI TCGCGA 1 cut(s) 1211
RsaI GTAC 2 cut(s) 554, 1175
RsaNI GTAC 2 cut(s) 553, 1174
RseI CAYNNNNRTG 1 cut(s) 1193
SalI GTCGAC 2 cut(s) 917, 1076
SapI GCTCTTC 2 cut(s) 761, 1178
SaqAI TTAA 2 cut(s) 261, 710
SatI GCNGC 4 cut(s) 14, 302, 354, 680
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 1 cut(s) 1094
SchI GAGTC 4 cut(s) 575, 636, 749, 785
ScrFI CCNGG 1 cut(s) 828
SduI GDGCHC 3 cut(s) 869, 1011, 1190
SfaNI GCATC 1 cut(s) 25
SfcI CTRYAG 1 cut(s) 225
SfoI GGCGCC 1 cut(s) 683
SmiMI CAYNNNNRTG 1 cut(s) 1193
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
SnaBI TACGTA 1 cut(s) 361
Sse9I AATT 5 cut(s) 215, 232, 604, 778, 1276
SseBI AGGCCT 1 cut(s) 815
SsiI CCGC 8 cut(s) 171, 299, 302, 341, 679, 752, 892, 922
SspDI GGCGCC 1 cut(s) 681
SspMI CTAG 2 cut(s) 633, 954
StuI AGGCCT 1 cut(s) 815
StyD4I CCNGG 1 cut(s) 826
TaaI ACNGT 4 cut(s) 88, 208, 283, 772
TaiI ACGT 4 cut(s) 363, 529, 724, 786
TaqI TCGA 7 cut(s) 416, 841, 912, 918, 1077, 1085, 1106
TaqII GACCGA 2 cut(s) 827, 861
TasI AATT 5 cut(s) 215, 232, 604, 778, 1276
TatI WGTACW 2 cut(s) 552, 1173
TauI GCSGC 2 cut(s) 304, 682
TfiI GAWTC 3 cut(s) 123, 200, 392
Tru1I TTAA 2 cut(s) 261, 710
Tru9I TTAA 2 cut(s) 261, 710
TscAI CASTG 1 cut(s) 911
TseFI GTSAC 2 cut(s) 643, 1126
TseI GCWGC 2 cut(s) 13, 353
Tsp45I GTSAC 2 cut(s) 643, 1126
TspDTI ATGAA 6 cut(s) 17, 279, 469, 557, 558, 1210
TspGWI ACGGA 3 cut(s) 954, 1092, 1136
TspRI CASTG 1 cut(s) 911
XapI RAATTY 1 cut(s) 604
XceI RCATGY 1 cut(s) 1170
XcmI CCANNNNNNNNNTGG 2 cut(s) 382, 700
XmiI GTMKAC 3 cut(s) 210, 918, 1077
XspI CTAG 2 cut(s) 633, 954
Zsp2I ATGCAT 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.