Rorug06G0471300

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016847
Physical Location & Seq
Reverse (-)
59431982 .. 59432610
629 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0471300.1

Sequence Viewer

Length: 378 bp
ATGGTTAGGGATGTCGATCTTCCCTTTTCTGAACAAGAAATCGAACGATCTGCTTCTTTTTTTGTACCCGGCATGACCTACTGCCCTGAGCAGAAGGCCATTAAGAAGGTCCCAATTCACTGTATCAGCAAGGAGCAAGTCTGCGATTTTTTCAAAAGATTTGATAGGAACGGTGACGGCAAACTCTGCAAGGAAGAGATCAAGGCAGCCTTTCGGAAACTCGGATCACGTTGGAGCTCTTACAGAGCCTGGAGAGCATTAGACCATGCAGATTCCAATGGCGATGGCATCATCTCTAATGAGGAGCTCGACGACCTTATCAACTATGCCCTAGATTGTGGTTATAAGCTATTAGCGCCACATATATGTGTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

125

Amino Acids

14.32

Weight (kDa)

5.91

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 49 - 75 3.1e-07 EF-hand domain
EF-hand_1 PF00036 49 - 74 3.3e-06 EF hand domain
EF-hand_5 PF13202 49 - 69 3.6e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016847)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11810
malus_domestica MD14G1228400.v1.1 MD14G1228500.v1.1
prunus_persica Prupe.5G223500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0185791
rosa_laevigata RLG00000004883
rosa_multiflora Rmu_co8078508.1_g000001 Rmu_sc0002121.1_g000003
rosa_rugosa Rorug06G0471300
rosa_samantha Rh7AG071000 Rh7BG076800 Rh7CG076800 Rh7DG076700
rosa_wichuraiana Rw7G006400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 345, 376
AclWI GGATC 1 cut(s) 232
AfaI GTAC 1 cut(s) 66
AgsI TTSAA 1 cut(s) 154
AjnI CCWGG 1 cut(s) 248
AluBI AGCT 3 cut(s) 237, 307, 349
AluI AGCT 3 cut(s) 237, 307, 349
Alw21I GWGCWC 2 cut(s) 239, 309
AlwI GGATC 1 cut(s) 232
AlwNI CAGNNNCTG 1 cut(s) 249
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 206
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 1 cut(s) 185
AvaII GGWCC 1 cut(s) 109
BanII GRGCYC 2 cut(s) 239, 309
Bbv12I GWGCWC 2 cut(s) 239, 309
BbvI GCAGC 1 cut(s) 218
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 193
BcgI CGANNNNNNTGC 2 cut(s) 32, 66
BciT130I CCWGG 1 cut(s) 250
BcnI CCSGG 1 cut(s) 69
BfaI CTAG 1 cut(s) 332
BfoI RGCGCY 1 cut(s) 359
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
Bme1390I CCNGG 2 cut(s) 69, 250
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 2 cut(s) 69, 250
BmsI GCATC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 271
Bpu10I CCTNAGC 1 cut(s) 87
BpuMI CCSGG 1 cut(s) 69
BsaBI GATNNNNATC 1 cut(s) 15
Bse8I GATNNNNATC 1 cut(s) 15
BseBI CCWGG 1 cut(s) 250
BseGI GGATG 1 cut(s) 16
BseJI GATNNNNATC 1 cut(s) 15
BseMII CTCAG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 317
BseXI GCAGC 1 cut(s) 218
BshFI GGCC 1 cut(s) 98
BsiHKAI GWGCWC 2 cut(s) 239, 309
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 239, 309
Bsp143I GATC 4 cut(s) 16, 47, 198, 224
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 111
BspPI GGATC 1 cut(s) 232
BssMI GATC 4 cut(s) 16, 47, 198, 224
Bst2UI CCWGG 1 cut(s) 250
Bst4CI ACNGT 2 cut(s) 122, 173
Bst6I CTCTTC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 186
BstDEI CTNAG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 16
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 4 cut(s) 19, 50, 201, 227
BstMBI GATC 4 cut(s) 16, 47, 198, 224
BstMWI GCNNNNNNNGC 3 cut(s) 186, 254, 355
BstNI CCWGG 1 cut(s) 250
BstSCI CCNGG 2 cut(s) 67, 248
BstV1I GCAGC 1 cut(s) 218
BsuRI GGCC 1 cut(s) 98
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 118
CaiI CAGNNNCTG 1 cut(s) 249
CfoI GCGC 1 cut(s) 358
Cfr13I GGNCC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 65
CviAII CATG 2 cut(s) 73, 266
CviJI RGCY 6 cut(s) 98, 209, 237, 248, 307, 349
CviKI_1 RGCY 6 cut(s) 98, 209, 237, 248, 307, 349
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 1 cut(s) 87
DpnI GATC 4 cut(s) 18, 49, 200, 226
DpnII GATC 4 cut(s) 16, 47, 198, 224
Eam1104I CTCTTC 1 cut(s) 189
EarI CTCTTC 1 cut(s) 189
Ecl136II GAGCTC 2 cut(s) 237, 307
Eco24I GRGCYC 2 cut(s) 239, 309
Eco47I GGWCC 1 cut(s) 109
Eco53kI GAGCTC 2 cut(s) 237, 307
EcoICRI GAGCTC 2 cut(s) 237, 307
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 248
EcoT38I GRGCYC 2 cut(s) 239, 309
FaeI CATG 2 cut(s) 76, 269
FaiI YATR 8 cut(s) 74, 267, 327, 345, 363, 365, 367, 376
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 1 cut(s) 95
FatI CATG 2 cut(s) 72, 265
Fnu4HI GCNGC 1 cut(s) 207
FokI GGATG 1 cut(s) 23
FriOI GRGCYC 2 cut(s) 239, 309
Fsp4HI GCNGC 1 cut(s) 207
FspBI CTAG 1 cut(s) 332
GlaI GCGC 1 cut(s) 357
GluI GCNGC 1 cut(s) 207
GsuI CTGGAG 1 cut(s) 271
HaeII RGCGCY 1 cut(s) 359
HaeIII GGCC 1 cut(s) 98
HapII CCGG 1 cut(s) 69
HhaI GCGC 1 cut(s) 358
Hin1II CATG 2 cut(s) 76, 269
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HinfI GANTC 1 cut(s) 272
HpaII CCGG 1 cut(s) 69
HphI GGTGA 1 cut(s) 185
Hpy188I TCNGA 3 cut(s) 31, 216, 224
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 2 cut(s) 88, 100
HpyCH4III ACNGT 2 cut(s) 122, 173
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 2 cut(s) 189, 269
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 254, 355
HpyF3I CTNAG 1 cut(s) 87
HpySE526I ACGT 1 cut(s) 229
Hsp92II CATG 2 cut(s) 76, 269
HspAI GCGC 1 cut(s) 356
Kzo9I GATC 4 cut(s) 16, 47, 198, 224
LmnI GCTCC 3 cut(s) 133, 234, 304
LpnPI CCDG 4 cut(s) 82, 99, 235, 262
Lsp1109I GCAGC 1 cut(s) 218
LweI GCATC 1 cut(s) 297
MaeI CTAG 1 cut(s) 332
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 1 cut(s) 173
MalI GATC 4 cut(s) 18, 49, 200, 226
MboI GATC 4 cut(s) 16, 47, 198, 224
MboII GAAGA 2 cut(s) 11, 206
MhlI GDGCHC 2 cut(s) 239, 309
MluCI AATT 1 cut(s) 114
MmeI TCCRAC 1 cut(s) 212
MnlI CCTC 1 cut(s) 295
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 2 cut(s) 364, 366
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 2 cut(s) 69, 250
MvaI CCWGG 1 cut(s) 250
MwoI GCNNNNNNNGC 3 cut(s) 186, 254, 355
NciI CCSGG 1 cut(s) 69
NdeII GATC 4 cut(s) 16, 47, 198, 224
NlaIII CATG 2 cut(s) 76, 269
NlaIV GGNNCC 1 cut(s) 111
NmuCI GTSAC 1 cut(s) 173
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 1 cut(s) 208
PpuMI RGGWCCY 1 cut(s) 109
PsiI TTATAA 2 cut(s) 345, 376
Psp124BI GAGCTC 2 cut(s) 239, 309
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 248
PspGI CCWGG 1 cut(s) 248
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 1 cut(s) 109
PspPPI RGGWCCY 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 249
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
RseI CAYNNNNRTG 2 cut(s) 364, 366
SacI GAGCTC 2 cut(s) 239, 309
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 4 cut(s) 16, 47, 198, 224
Sau96I GGNCC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 69, 250
SduI GDGCHC 2 cut(s) 239, 309
SetI ASST 7 cut(s) 80, 111, 232, 239, 309, 318, 351
SfaNI GCATC 1 cut(s) 297
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 2 cut(s) 364, 366
Sse9I AATT 1 cut(s) 114
SspMI CTAG 1 cut(s) 332
SstI GAGCTC 2 cut(s) 239, 309
StyD4I CCNGG 2 cut(s) 67, 248
TaaI ACNGT 2 cut(s) 122, 173
TaiI ACGT 1 cut(s) 232
TaqI TCGA 3 cut(s) 15, 42, 309
TasI AATT 1 cut(s) 114
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 125
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 173
TspRI CASTG 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 109
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.