Rw7G006400

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016847
Physical Location & Seq
Forward (+)
5457259 .. 5461041
3783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G006400.1

Sequence Viewer

Length: 1278 bp
ATGAAGAACCAGCAGCAACAAAACCCACCACCCTGTTTTCTCAAACACTTCGTCAAGGCTCAAATGCAAATCAACAAGATCATCTCTATTTTCCTCATCTTTGTCTCTGGCCTTGCCCTTGGAATCACCCTAAGCTTCTACATCAAAGACAGCCCTCTTCAAATCTACACTGCATTAGCTCAAGCGAAAACCAATTTCGATTCCACCATATACAAATTAGTCCCTATAGATTCTCCCCCACCACCACCACCATCTTCTCCTGTTAAAAATGAAACCAATATCACCACCAAACCCCTCTCTTCACCGCCGCCTCCTCCGCCGGCTTCTCCACCTCCGCCAGTCGAGTTGAAAAATTACGTAAAGGTGCCAAGTGCCATGCATGGTATGGAGGATTCTGAGTTATTGTGGAGAGCTTCGATGGTGCCAAAAAGAAGTGGGGCATTGCCATTCAAGAACACCAAGCCAAAAGTGGCCTTCATGTTTTTGACCAGAGAGGATTTGGCTTTGGCTCCTTTATGGGAACGTTTCTTTAAAGGAAATGAAGGGCTTTACTCTATCTACGTCCATGCCAGTCCAACTTCCGGCGAGACAATGCCTGAAAATTCTGTGTTTCACGGCAGAAGAGTCCCGAGTAAGAATGTGACGTGGGGTGAGCCAAACATGGTGCAAGCTGAGCGCCGCCTACTAGCCAATGCACTGCTGGACTTGTCCAACCAACGTTTCATTCTTCTCTCGGAGTCGTGCATACCACTATTCAACTTCAAAACGGTCTATGATTACGTTATGGACTCAACCAAAACATTTGTGGAGGCCTACGACTTGCCCGGTCCAGTGGGCCGAGACAGATATAGGAAACAAATGAGCCCAACAATCAAGCTTGAGCAATGGCGGAAGGGGTCGCAGTGGTTCGAGGTGGACCGCCAGTTGGCACTGGAGGTGGTTTCGGACAGACGGTATTTTCCGCTGTTTGAGAAGTACTGCGTCCCCTCGTGCTACTCGGATGAGCACTACTTGCCTACTTACACGAACATCAAATTTTGGAAGAAAAACTCGAACCGGACTTTGCATTGGGTTGACTGGTCGAGAGGGGGGCCACATCCGTCGAGGTATCAGAGGACGGATGTGACTCATGAGTTCTTGAGCAAGATGAGATTTGGAACAACATGTGAGTACAATGGGAACAACACTAACATATGTCATTTGTTTGCAAGAAAGTTCATGCCTAACACTTTGGATAGGCTTTTGAGGTTTGCTCCCAAGCTCATGCAATTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

425

Amino Acids

48.97

Weight (kDa)

9.47

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 157 - 384 1.9e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016847)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11810
malus_domestica MD14G1228400.v1.1 MD14G1228500.v1.1
prunus_persica Prupe.5G223500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0185791
rosa_laevigata RLG00000004883
rosa_multiflora Rmu_co8078508.1_g000001 Rmu_sc0002121.1_g000003
rosa_rugosa Rorug06G0471300
rosa_samantha Rh7AG071000 Rh7BG076800 Rh7CG076800 Rh7DG076700
rosa_wichuraiana Rw7G006400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 364, 421
AciI CCGC 8 cut(s) 305, 308, 317, 335, 679, 889, 919, 962
AclI AACGTT 2 cut(s) 523, 718
AcsI RAATTY 2 cut(s) 601, 1034
AfaI GTAC 2 cut(s) 977, 1172
AfiI CCNNNNNNNGG 2 cut(s) 581, 925
AflIII ACRYGT 1 cut(s) 1163
AgsI TTSAA 5 cut(s) 161, 349, 451, 757, 763
AjiI CACGTC 1 cut(s) 645
AjuI GAANNNNNNNTTGG 4 cut(s) 704, 708, 736, 740
AluBI AGCT 6 cut(s) 135, 179, 413, 671, 877, 1261
AluI AGCT 6 cut(s) 135, 179, 413, 671, 877, 1261
Alw21I GWGCWC 1 cut(s) 1008
Alw26I GTCTC 3 cut(s) 109, 581, 834
Ama87I CYCGRG 1 cut(s) 628
AoxI GGCC 5 cut(s) 109, 471, 810, 835, 1091
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 2 cut(s) 601, 1034
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AspLEI GCGC 1 cut(s) 678
AspS9I GGNCC 4 cut(s) 827, 835, 916, 1091
AsuC2I CCSGG 1 cut(s) 825
AsuHPI GGTGA 4 cut(s) 118, 274, 294, 662
AvaI CYCGRG 1 cut(s) 628
AvaII GGWCC 2 cut(s) 827, 916
BanI GGYRCC 2 cut(s) 364, 421
BanII GRGCYC 1 cut(s) 866
BauI CACGAG 1 cut(s) 988
Bbv12I GWGCWC 1 cut(s) 1008
BbvI GCAGC 1 cut(s) 25
BccI CCATC 2 cut(s) 259, 412
BceAI ACGGC 1 cut(s) 631
BcnI CCSGG 1 cut(s) 825
BcoDI GTCTC 3 cut(s) 109, 581, 834
BfaI CTAG 1 cut(s) 686
BfmI CTRYAG 1 cut(s) 225
BfoI RGCGCY 1 cut(s) 679
BisI GCNGC 3 cut(s) 14, 308, 679
BlpI GCTNAGC 1 cut(s) 672
BlsI GCNGC 3 cut(s) 15, 309, 680
BmcAI AGTACT 1 cut(s) 977
Bme1390I CCNGG 1 cut(s) 825
Bme18I GGWCC 2 cut(s) 827, 916
BmeT110I CYCGRG 1 cut(s) 628
BmgBI CACGTC 1 cut(s) 645
BmgT120I GGNCC 4 cut(s) 827, 835, 916, 1091
BmiI GGNNCC 4 cut(s) 366, 423, 510, 1092
BmrFI CCNGG 1 cut(s) 825
BplI GAGNNNNNCTC 2 cut(s) 1237, 1269
BpmI CTGGAG 1 cut(s) 953
Bpu10I CCTNAGC 1 cut(s) 131
Bpu1102I GCTNAGC 1 cut(s) 672
BpuEI CTTGAG 3 cut(s) 165, 899, 1159
BpuMI CCSGG 1 cut(s) 825
BsaAI YACGTR 1 cut(s) 358
BsaJI CCNNGG 1 cut(s) 118
BsaWI WCCGGW 1 cut(s) 1056
Bsc4I CCNNNNNNNGG 2 cut(s) 581, 925
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 6 cut(s) 338, 570, 830, 922, 936, 1082
Bse3DI GCAATG 2 cut(s) 440, 890
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 3 cut(s) 1006, 1096, 1126
BseLI CCNNNNNNNGG 2 cut(s) 581, 925
BseMI GCAATG 2 cut(s) 440, 890
BseMII CTCAG 2 cut(s) 387, 663
BseNI ACTGG 6 cut(s) 338, 570, 830, 922, 936, 1082
BseRI GAGGAG 1 cut(s) 303
BseXI GCAGC 1 cut(s) 25
BshFI GGCC 5 cut(s) 111, 473, 812, 837, 1093
BshNI GGYRCC 2 cut(s) 364, 421
BsiHKAI GWGCWC 1 cut(s) 1008
BsiHKCI CYCGRG 1 cut(s) 628
BsiSI CCGG 4 cut(s) 320, 582, 825, 1057
BslFI GGGAC 3 cut(s) 206, 611, 968
BslI CCNNNNNNNGG 2 cut(s) 581, 925
BsmAI GTCTC 3 cut(s) 109, 581, 834
BsmFI GGGAC 3 cut(s) 206, 611, 968
BsnI GGCC 5 cut(s) 111, 473, 812, 837, 1093
BsoBI CYCGRG 1 cut(s) 628
Bsp1286I GDGCHC 2 cut(s) 866, 1008
Bsp143I GATC 1 cut(s) 78
Bsp1720I GCTNAGC 1 cut(s) 672
BspACI CCGC 8 cut(s) 305, 308, 317, 335, 679, 889, 919, 962
BspANI GGCC 5 cut(s) 111, 473, 812, 837, 1093
BspCNI CTCAG 2 cut(s) 388, 664
BspHI TCATGA 1 cut(s) 1129
BspLI GGNNCC 4 cut(s) 366, 423, 510, 1092
BspT107I GGYRCC 2 cut(s) 364, 421
BsrDI GCAATG 2 cut(s) 440, 890
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 6 cut(s) 338, 570, 830, 922, 936, 1082
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 1 cut(s) 78
BssSI CACGAG 1 cut(s) 988
BssT1I CCWWGG 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 988
Bst4CI ACNGT 2 cut(s) 769, 954
Bst6I CTCTTC 3 cut(s) 162, 304, 616
BstAPI GCANNNNNTGC 1 cut(s) 1012
BstBAI YACGTR 1 cut(s) 358
BstC8I GCNNGC 2 cut(s) 321, 669
BstDEI CTNAG 3 cut(s) 131, 396, 672
BstF5I GGATG 3 cut(s) 1006, 1096, 1126
BstH2I RGCGCY 1 cut(s) 679
BstHHI GCGC 1 cut(s) 678
BstKTI GATC 1 cut(s) 81
BstMAI GTCTC 3 cut(s) 109, 581, 834
BstMBI GATC 1 cut(s) 78
BstMWI GCNNNNNNNGC 3 cut(s) 316, 673, 1012
BstNSI RCATGY 1 cut(s) 1167
BstSCI CCNGG 1 cut(s) 823
BstSFI CTRYAG 1 cut(s) 225
BstSNI TACGTA 1 cut(s) 358
BstV1I GCAGC 1 cut(s) 25
BsuRI GGCC 5 cut(s) 111, 473, 812, 837, 1093
BtrI CACGTC 1 cut(s) 645
BtsCI GGATG 3 cut(s) 1006, 1096, 1126
BtsI GCAGTG 3 cut(s) 168, 695, 908
BtsIMutI CAGTG 5 cut(s) 168, 695, 837, 908, 929
Cac8I GCNNGC 2 cut(s) 321, 669
CciI TCATGA 1 cut(s) 1129
CfoI GCGC 1 cut(s) 678
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 4 cut(s) 827, 835, 916, 1091
CseI GACGC 1 cut(s) 970
Csp6I GTAC 2 cut(s) 976, 1171
CviAII CATG 9 cut(s) 376, 380, 478, 566, 661, 1130, 1164, 1219, 1264
CviQI GTAC 2 cut(s) 976, 1171
DdeI CTNAG 3 cut(s) 131, 396, 672
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
DraI TTTAAA 1 cut(s) 532
Eam1104I CTCTTC 3 cut(s) 162, 304, 616
EarI CTCTTC 3 cut(s) 162, 304, 616
EciI GGCGGA 3 cut(s) 306, 324, 904
Eco105I TACGTA 1 cut(s) 358
Eco130I CCWWGG 1 cut(s) 118
Eco147I AGGCCT 1 cut(s) 812
Eco24I GRGCYC 1 cut(s) 866
Eco47I GGWCC 2 cut(s) 827, 916
Eco88I CYCGRG 1 cut(s) 628
EcoT14I CCWWGG 1 cut(s) 118
EcoT22I ATGCAT 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 866
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 9 cut(s) 379, 383, 481, 569, 664, 1133, 1167, 1222, 1267
FaqI GGGAC 3 cut(s) 206, 611, 968
FatI CATG 9 cut(s) 375, 379, 477, 565, 660, 1129, 1163, 1218, 1263
FauNDI CATATG 1 cut(s) 1193
Fnu4HI GCNGC 3 cut(s) 14, 308, 679
FokI GGATG 3 cut(s) 1013, 1083, 1133
FriOI GRGCYC 1 cut(s) 866
Fsp4HI GCNGC 3 cut(s) 14, 308, 679
FspBI CTAG 1 cut(s) 686
GlaI GCGC 1 cut(s) 677
GluI GCNGC 3 cut(s) 14, 308, 679
GsuI CTGGAG 1 cut(s) 953
HaeII RGCGCY 1 cut(s) 679
HaeIII GGCC 5 cut(s) 111, 473, 812, 837, 1093
HapII CCGG 4 cut(s) 320, 582, 825, 1057
HgaI GACGC 1 cut(s) 970
HhaI GCGC 1 cut(s) 678
Hin1II CATG 9 cut(s) 379, 383, 481, 569, 664, 1133, 1167, 1222, 1267
Hin6I GCGC 1 cut(s) 676
HinP1I GCGC 1 cut(s) 676
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HindIII AAGCTT 2 cut(s) 133, 875
HinfI GANTC 8 cut(s) 123, 200, 230, 392, 624, 737, 788, 1126
HpaII CCGG 4 cut(s) 320, 582, 825, 1057
HphI GGTGA 4 cut(s) 118, 274, 294, 662
Hpy166II GTNNAC 2 cut(s) 916, 1075
Hpy188I TCNGA 5 cut(s) 397, 736, 946, 1000, 1113
Hpy188III TCNNGA 5 cut(s) 451, 628, 1083, 1130, 1138
Hpy8I GTNNAC 2 cut(s) 916, 1075
Hpy99I CGWCG 1 cut(s) 1105
HpyAV CCTTC 3 cut(s) 484, 536, 886
HpyCH4III ACNGT 2 cut(s) 769, 954
HpyCH4IV ACGT 6 cut(s) 357, 523, 561, 644, 718, 780
HpyCH4V TGCA 9 cut(s) 67, 173, 379, 667, 695, 744, 1066, 1208, 1267
HpyF10VI GCNNNNNNNGC 3 cut(s) 316, 673, 1012
HpyF3I CTNAG 3 cut(s) 131, 396, 672
HpySE526I ACGT 6 cut(s) 357, 523, 561, 644, 718, 780
Hsp92II CATG 9 cut(s) 379, 383, 481, 569, 664, 1133, 1167, 1222, 1267
HspAI GCGC 1 cut(s) 676
KroI GCCGGC 1 cut(s) 319
KroNI GCCGGC 1 cut(s) 321
Kzo9I GATC 1 cut(s) 78
LmnI GCTCC 2 cut(s) 514, 1258
Lsp1109I GCAGC 1 cut(s) 25
MaeI CTAG 1 cut(s) 686
MaeII ACGT 6 cut(s) 357, 523, 561, 644, 718, 780
MaeIII GTNAC 2 cut(s) 640, 1123
MalI GATC 1 cut(s) 80
MboI GATC 1 cut(s) 78
MboII GAAGA 7 cut(s) 16, 149, 246, 291, 633, 719, 1054
MhlI GDGCHC 2 cut(s) 866, 1008
MluCI AATT 6 cut(s) 193, 215, 352, 601, 1034, 1268
MlyI GAGTC 4 cut(s) 633, 746, 782, 1120
MmeI TCCRAC 2 cut(s) 599, 735
Mph1103I ATGCAT 1 cut(s) 381
MroNI GCCGGC 1 cut(s) 319
MseI TTAA 2 cut(s) 264, 531
MspA1I CMGCKG 1 cut(s) 964
MspI CCGG 4 cut(s) 320, 582, 825, 1057
MspR9I CCNGG 1 cut(s) 825
MwoI GCNNNNNNNGC 3 cut(s) 316, 673, 1012
NaeI GCCGGC 1 cut(s) 321
NciI CCSGG 1 cut(s) 825
NdeI CATATG 1 cut(s) 1193
NdeII GATC 1 cut(s) 78
NgoMIV GCCGGC 1 cut(s) 319
NlaIII CATG 9 cut(s) 379, 383, 481, 569, 664, 1133, 1167, 1222, 1267
NlaIV GGNNCC 4 cut(s) 366, 423, 510, 1092
NmeAIII GCCGAG 1 cut(s) 863
NmuCI GTSAC 2 cut(s) 640, 1123
NsiI ATGCAT 1 cut(s) 381
NspI RCATGY 1 cut(s) 1167
PagI TCATGA 1 cut(s) 1129
PceI AGGCCT 1 cut(s) 812
PciI ACATGT 1 cut(s) 1163
PdiI GCCGGC 1 cut(s) 321
PfeI GAWTC 4 cut(s) 123, 200, 230, 392
PkrI GCNGC 3 cut(s) 15, 309, 680
PleI GAGTC 4 cut(s) 632, 745, 782, 1120
PpsI GAGTC 4 cut(s) 632, 745, 782, 1120
Ppu21I YACGTR 1 cut(s) 358
PscI ACATGT 1 cut(s) 1163
Psp1406I AACGTT 2 cut(s) 523, 718
PspN4I GGNNCC 4 cut(s) 366, 423, 510, 1092
PspPI GGNCC 4 cut(s) 827, 835, 916, 1091
RsaI GTAC 2 cut(s) 977, 1172
RsaNI GTAC 2 cut(s) 976, 1171
SaqAI TTAA 2 cut(s) 264, 531
SatI GCNGC 3 cut(s) 14, 308, 679
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 4 cut(s) 827, 835, 916, 1091
ScaI AGTACT 1 cut(s) 977
SchI GAGTC 4 cut(s) 633, 746, 782, 1120
ScrFI CCNGG 1 cut(s) 825
SduI GDGCHC 2 cut(s) 866, 1008
SfcI CTRYAG 1 cut(s) 225
SinI GGWCC 2 cut(s) 827, 916
SmlI CTYRAG 3 cut(s) 180, 878, 1138
SmoI CTYRAG 3 cut(s) 180, 878, 1138
SnaBI TACGTA 1 cut(s) 358
Sse9I AATT 6 cut(s) 193, 215, 352, 601, 1034, 1268
SseBI AGGCCT 1 cut(s) 812
SsiI CCGC 8 cut(s) 305, 308, 317, 335, 679, 889, 919, 962
SspMI CTAG 1 cut(s) 686
StuI AGGCCT 1 cut(s) 812
StyD4I CCNGG 1 cut(s) 823
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 2 cut(s) 769, 954
TaiI ACGT 6 cut(s) 360, 526, 564, 647, 721, 783
TaqI TCGA 8 cut(s) 198, 342, 416, 909, 1052, 1082, 1103, 1272
TasI AATT 6 cut(s) 193, 215, 352, 601, 1034, 1268
TatI WGTACW 2 cut(s) 975, 1170
TauI GCSGC 2 cut(s) 310, 681
TfiI GAWTC 4 cut(s) 123, 200, 230, 392
Tru1I TTAA 2 cut(s) 264, 531
Tru9I TTAA 2 cut(s) 264, 531
TscAI CASTG 5 cut(s) 175, 702, 837, 908, 936
TseFI GTSAC 2 cut(s) 640, 1123
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 2 cut(s) 640, 1123
TspDTI ATGAA 6 cut(s) 17, 285, 466, 555, 712, 1207
TspGWI ACGGA 2 cut(s) 1089, 1133
TspRI CASTG 5 cut(s) 175, 702, 837, 908, 936
VpaK11BI GGWCC 2 cut(s) 827, 916
XapI RAATTY 2 cut(s) 601, 1034
XceI RCATGY 1 cut(s) 1167
XcmI CCANNNNNNNNNTGG 5 cut(s) 382, 466, 496, 697, 802
XspI CTAG 1 cut(s) 686
ZrmI AGTACT 1 cut(s) 977
Zsp2I ATGCAT 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.