Prupe.5G223500_v2.0.a1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0016847
Physical Location & Seq
Forward (+)
17331364 .. 17334903
3540 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G223500.1

Sequence Viewer

Length: 1260 bp
ATGAAGAACAAACCGCAGCAGCAGCAGCAGCAGCAGCAACCACCCTCATCAATTCCCAAGCATTTCCTCAATGTCCAAATACACCTCCATAAAATCATCTCCAACTTTCTCATCTTCGCCTTCGGTTTAGCCATCGGCGTCTCCATCAGTTTCTACCTCAAAGATTTTCCCTTCCAGCTCTACGCCACCCAAGCCAAATACTCTCGTTCATCAAACACTCCATTGTTATTCTCTTCTGCTCCACCACCACTACCACCGCCGCCGCTGCCAACTCTCAATATCCAAATAAATAAAACCAAAACTTCTGCTGCGCCTCCCCCACCGCCAATCGGCTTAAAAGAATACACAAAGCCACCCAACATTATGCACGACATGGACGACGCTGAGTTGCTGTGGAGAGCTTCACTGGTGCCTCGCCGGCGTGGGCTGCCGTTCAAGCGCACCCCAAAAGTGGCGTTCATGTTCTTGACAAGAGGGCCTCTGGCATTGGCTCCGTTCTGGGAAATGTTCTTCAAAGGCCATGAAGGGCTCTACTCTATCTATGTCCACGCCAATCCGGATTTCAACGACACCATGCCTCAAAACTCTGTTTTTTATGGCAGAAGGGTCCCAAGTAAGAATGTGACATGGGGTGAGCCTAACATGGTGCAAGCAGAGCGACGCCTATTAGCCAATGCCTTGCTTGACTTCTCCAACCAACGTTTTGTGCTGCTCTCAGAGTCTTGCATACCGCTGTTTAATTTCAAAGTCATCTACGATTATCTGATGGGCTCAACCCAGACATTTGTGGAGGTCTACGACTTACCCGGCCCAGTGGGCCGAGACAGGTACAGGCCCAAAATGAGCCCACAAATCACGCTCGGCCAATGGCGGAAGGGCTCGCAGTGGTTCGAGGTGGACCGTGTGATTGCGGTGGAGGTGGTTTCCGATCAAAAGTATTTTCCACTGTTTACAAAGCACTGCAAGCCCTCCTGCTATTCTGACGAGCACTACTTGCCTACGTACGTGAACATCAAGTTTAGGAAGAAAAATTCGAATCGGACGTTGACTTGGGTTGACTGGTCAAGGGGTGGGCCCCATCCGTCTAAGTTTATGAGAACAGATGTGACTGTTGAGTTCTTGGAGAAGCTGAGGCATGGAACGAAATGCGAGTACAATGGGAAGACCACCGATGTTTGCCACTTGTTTGCCAGAAAATTCTTGCCCAACACTTTGGATAGGCTGCTGAGGTTTGGCCCAAAGCTCATGAAATTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

420

Amino Acids

48.5

Weight (kDa)

9.83

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016847)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g11810
malus_domestica MD14G1228400.v1.1 MD14G1228500.v1.1
prunus_persica Prupe.5G223500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0185791
rosa_laevigata RLG00000004883
rosa_multiflora Rmu_co8078508.1_g000001 Rmu_sc0002121.1_g000003
rosa_rugosa Rorug06G0471300
rosa_samantha Rh7AG071000 Rh7BG076800 Rh7CG076800 Rh7DG076700
rosa_wichuraiana Rw7G006400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 409
AccI GTMKAC 1 cut(s) 795
AccIII TCCGGA 1 cut(s) 556
AciI CCGC 8 cut(s) 14, 257, 260, 263, 323, 731, 871, 911
AclI AACGTT 1 cut(s) 700
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 3 cut(s) 1030, 1196, 1250
AcyI GRCGYC 2 cut(s) 138, 661
AfaI GTAC 3 cut(s) 830, 1004, 1154
AfiI CCNNNNNNNGG 2 cut(s) 329, 451
AgsI TTSAA 5 cut(s) 436, 514, 565, 745, 1255
AjuI GAANNNNNNNTTGG 2 cut(s) 545, 577
AluBI AGCT 4 cut(s) 178, 401, 1129, 1243
AluI AGCT 4 cut(s) 178, 401, 1129, 1243
Alw21I GWGCWC 1 cut(s) 990
Alw26I GTCTC 2 cut(s) 145, 816
Aor13HI TCCGGA 1 cut(s) 556
AoxI GGCC 8 cut(s) 476, 517, 808, 817, 833, 862, 1073, 1234
ApaI GGGCCC 1 cut(s) 1077
ApoI RAATTY 3 cut(s) 1030, 1196, 1250
AspLEI GCGC 2 cut(s) 313, 441
AspS9I GGNCC 9 cut(s) 476, 607, 809, 817, 834, 898, 1073, 1074, 1235
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 1 cut(s) 644
AsuII TTCGAA 1 cut(s) 1034
AvaII GGWCC 2 cut(s) 607, 898
BaeGI GKGCMC 1 cut(s) 1077
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 5 cut(s) 531, 773, 848, 881, 1077
BbsI GAAGAC 1 cut(s) 1169
Bbv12I GWGCWC 1 cut(s) 990
BbvCI CCTCAGC 2 cut(s) 1130, 1226
BccI CCATC 4 cut(s) 140, 152, 760, 1086
BceAI ACGGC 1 cut(s) 415
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 2 cut(s) 145, 816
BglI GCCNNNNNGGC 2 cut(s) 418, 816
Bme1390I CCNGG 1 cut(s) 807
Bme18I GGWCC 2 cut(s) 607, 898
BmgT120I GGNCC 9 cut(s) 476, 607, 809, 817, 834, 898, 1073, 1074, 1235
BmiI GGNNCC 6 cut(s) 411, 492, 608, 609, 1075, 1076
BmrFI CCNGG 1 cut(s) 807
BmrI ACTGGG 1 cut(s) 806
BmuI ACTGGG 1 cut(s) 806
BpiI GAAGAC 1 cut(s) 1169
Bpu10I CCTNAGC 2 cut(s) 1130, 1226
Bpu14I TTCGAA 1 cut(s) 1034
BpuMI CCSGG 1 cut(s) 807
BsaAI YACGTR 2 cut(s) 1002, 1006
BsaHI GRCGYC 2 cut(s) 138, 661
BsaWI WCCGGW 1 cut(s) 556
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 451
Bse118I RCCGGY 1 cut(s) 417
Bse1I ACTGG 3 cut(s) 411, 812, 1064
BseAI TCCGGA 1 cut(s) 556
BseGI GGATG 1 cut(s) 1078
BseLI CCNNNNNNNGG 2 cut(s) 329, 451
BseMII CTCAG 4 cut(s) 375, 729, 1121, 1217
BseNI ACTGG 3 cut(s) 411, 812, 1064
BseSI GKGCMC 1 cut(s) 1077
BshFI GGCC 8 cut(s) 478, 519, 810, 819, 835, 864, 1075, 1236
BshNI GGYRCC 1 cut(s) 409
BsiHKAI GWGCWC 1 cut(s) 990
BsiSI CCGG 3 cut(s) 418, 557, 807
BsiWI CGTACG 1 cut(s) 1002
BslFI GGGAC 1 cut(s) 593
BslI CCNNNNNNNGG 2 cut(s) 329, 451
BsmAI GTCTC 2 cut(s) 145, 816
BsmBI CGTCTC 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 593
BsnI GGCC 8 cut(s) 478, 519, 810, 819, 835, 864, 1075, 1236
Bsp119I TTCGAA 1 cut(s) 1034
Bsp120I GGGCCC 1 cut(s) 1073
Bsp1286I GDGCHC 6 cut(s) 531, 773, 848, 881, 990, 1077
Bsp13I TCCGGA 1 cut(s) 556
Bsp143I GATC 1 cut(s) 928
BspACI CCGC 8 cut(s) 14, 257, 260, 263, 323, 731, 871, 911
BspANI GGCC 8 cut(s) 478, 519, 810, 819, 835, 864, 1075, 1236
BspCNI CTCAG 4 cut(s) 376, 728, 1122, 1218
BspEI TCCGGA 1 cut(s) 556
BspHI TCATGA 1 cut(s) 1245
BspLI GGNNCC 6 cut(s) 411, 492, 608, 609, 1075, 1076
BspT104I TTCGAA 1 cut(s) 1034
BspT107I GGYRCC 1 cut(s) 409
BsrFI RCCGGY 1 cut(s) 417
BsrI ACTGG 3 cut(s) 411, 812, 1064
BssAI RCCGGY 1 cut(s) 417
BssMI GATC 1 cut(s) 928
BssNI GRCGYC 2 cut(s) 138, 661
Bst4CI ACNGT 3 cut(s) 902, 948, 1111
Bst6I CTCTTC 1 cut(s) 238
BstACI GRCGYC 2 cut(s) 138, 661
BstAPI GCANNNNNTGC 1 cut(s) 994
BstBAI YACGTR 2 cut(s) 1002, 1006
BstBI TTCGAA 1 cut(s) 1034
BstC8I GCNNGC 4 cut(s) 419, 651, 881, 965
BstDEI CTNAG 5 cut(s) 384, 715, 1086, 1130, 1226
BstF5I GGATG 1 cut(s) 1078
BstHHI GCGC 2 cut(s) 313, 441
BstKTI GATC 1 cut(s) 931
BstMAI GTCTC 2 cut(s) 145, 816
BstMBI GATC 1 cut(s) 928
BstSCI CCNGG 1 cut(s) 805
BstSLI GKGCMC 1 cut(s) 1077
BstSNI TACGTA 1 cut(s) 1002
BstV2I GAAGAC 1 cut(s) 1169
BstXI CCANNNNNNTGG 1 cut(s) 1213
BsuRI GGCC 8 cut(s) 478, 519, 810, 819, 835, 864, 1075, 1236
BtsCI GGATG 1 cut(s) 1078
BtsI GCAGTG 2 cut(s) 890, 958
BtsIMutI CAGTG 5 cut(s) 404, 819, 890, 944, 958
Cac8I GCNNGC 4 cut(s) 419, 651, 881, 965
CciI TCATGA 1 cut(s) 1245
CfoI GCGC 2 cut(s) 313, 441
Cfr10I RCCGGY 1 cut(s) 417
Cfr13I GGNCC 9 cut(s) 476, 607, 809, 817, 834, 898, 1073, 1074, 1235
CseI GACGC 3 cut(s) 127, 389, 669
Csp6I GTAC 3 cut(s) 829, 1003, 1153
CviAII CATG 8 cut(s) 373, 460, 521, 574, 627, 643, 1136, 1246
CviQI GTAC 3 cut(s) 829, 1003, 1153
DdeI CTNAG 5 cut(s) 384, 715, 1086, 1130, 1226
DpnI GATC 1 cut(s) 930
DpnII GATC 1 cut(s) 928
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
EciI GGCGGA 1 cut(s) 886
Eco105I TACGTA 1 cut(s) 1002
Eco24I GRGCYC 5 cut(s) 531, 773, 848, 881, 1077
Eco47I GGWCC 2 cut(s) 607, 898
EcoO109I RGGNCCY 3 cut(s) 476, 607, 1074
EcoT38I GRGCYC 5 cut(s) 531, 773, 848, 881, 1077
Esp3I CGTCTC 1 cut(s) 145
FaeI CATG 8 cut(s) 376, 463, 524, 577, 630, 646, 1139, 1249
FaqI GGGAC 1 cut(s) 593
FatI CATG 8 cut(s) 372, 459, 520, 573, 626, 642, 1135, 1245
FblI GTMKAC 1 cut(s) 795
FokI GGATG 1 cut(s) 1065
FriOI GRGCYC 5 cut(s) 531, 773, 848, 881, 1077
GlaI GCGC 2 cut(s) 312, 440
HaeIII GGCC 8 cut(s) 478, 519, 810, 819, 835, 864, 1075, 1236
HapII CCGG 3 cut(s) 418, 557, 807
HgaI GACGC 3 cut(s) 127, 389, 669
HhaI GCGC 2 cut(s) 313, 441
Hin1I GRCGYC 2 cut(s) 138, 661
Hin1II CATG 8 cut(s) 376, 463, 524, 577, 630, 646, 1139, 1249
Hin6I GCGC 2 cut(s) 311, 439
HinP1I GCGC 2 cut(s) 311, 439
HincII GTYRAC 2 cut(s) 1047, 1057
HindII GTYRAC 2 cut(s) 1047, 1057
HinfI GANTC 2 cut(s) 719, 1036
HpaII CCGG 3 cut(s) 418, 557, 807
HphI GGTGA 1 cut(s) 644
Hpy166II GTNNAC 7 cut(s) 547, 796, 898, 951, 1009, 1047, 1057
Hpy188I TCNGA 5 cut(s) 718, 765, 928, 982, 1041
Hpy188III TCNNGA 3 cut(s) 466, 557, 1246
Hpy8I GTNNAC 7 cut(s) 547, 796, 898, 951, 1009, 1047, 1057
Hpy99I CGWCG 2 cut(s) 383, 663
HpyAV CCTTC 5 cut(s) 130, 181, 518, 597, 868
HpyCH4III ACNGT 3 cut(s) 902, 948, 1111
HpyCH4IV ACGT 4 cut(s) 700, 1001, 1005, 1043
HpyCH4V TGCA 4 cut(s) 367, 649, 726, 963
HpyF3I CTNAG 5 cut(s) 384, 715, 1086, 1130, 1226
HpySE526I ACGT 4 cut(s) 700, 1001, 1005, 1043
Hsp92I GRCGYC 2 cut(s) 138, 661
Hsp92II CATG 8 cut(s) 376, 463, 524, 577, 630, 646, 1139, 1249
HspAI GCGC 2 cut(s) 311, 439
KflI GGGWCCC 1 cut(s) 607
Kpn2I TCCGGA 1 cut(s) 556
KroI GCCGGC 1 cut(s) 417
KroNI GCCGGC 1 cut(s) 419
Kzo9I GATC 1 cut(s) 928
LmnI GCTCC 2 cut(s) 244, 496
MaeII ACGT 4 cut(s) 700, 1001, 1005, 1043
MaeIII GTNAC 2 cut(s) 622, 1105
MalI GATC 1 cut(s) 930
MboI GATC 1 cut(s) 928
MboII GAAGA 6 cut(s) 16, 106, 225, 502, 1036, 1174
MhlI GDGCHC 6 cut(s) 531, 773, 848, 881, 990, 1077
MluCI AATT 6 cut(s) 51, 739, 1030, 1196, 1250, 1255
MlyI GAGTC 1 cut(s) 728
MmeI TCCRAC 2 cut(s) 126, 717
MreI CGCCGGCG 1 cut(s) 417
MroI TCCGGA 1 cut(s) 556
MroNI GCCGGC 1 cut(s) 417
MseI TTAA 3 cut(s) 335, 738, 1258
MspA1I CMGCKG 2 cut(s) 265, 733
MspI CCGG 3 cut(s) 418, 557, 807
MspR9I CCNGG 1 cut(s) 807
NaeI GCCGGC 1 cut(s) 419
NciI CCSGG 1 cut(s) 807
NdeII GATC 1 cut(s) 928
NgoMIV GCCGGC 1 cut(s) 417
NlaIII CATG 8 cut(s) 376, 463, 524, 577, 630, 646, 1139, 1249
NlaIV GGNNCC 6 cut(s) 411, 492, 608, 609, 1075, 1076
NmeAIII GCCGAG 2 cut(s) 840, 845
NmuCI GTSAC 2 cut(s) 622, 1105
NspV TTCGAA 1 cut(s) 1034
PagI TCATGA 1 cut(s) 1245
PcsI WCGNNNNNNNCGW 2 cut(s) 375, 1040
PdiI GCCGGC 1 cut(s) 419
PfeI GAWTC 1 cut(s) 1036
Pfl23II CGTACG 1 cut(s) 1002
PleI GAGTC 1 cut(s) 727
PpsI GAGTC 1 cut(s) 727
Ppu21I YACGTR 2 cut(s) 1002, 1006
PpuMI RGGWCCY 1 cut(s) 607
Psp1406I AACGTT 1 cut(s) 700
Psp5II RGGWCCY 1 cut(s) 607
PspLI CGTACG 1 cut(s) 1002
PspN4I GGNNCC 6 cut(s) 411, 492, 608, 609, 1075, 1076
PspOMI GGGCCC 1 cut(s) 1073
PspPI GGNCC 9 cut(s) 476, 607, 809, 817, 834, 898, 1073, 1074, 1235
PspPPI RGGWCCY 1 cut(s) 607
RsaI GTAC 3 cut(s) 830, 1004, 1154
RsaNI GTAC 3 cut(s) 829, 1003, 1153
SaqAI TTAA 3 cut(s) 335, 738, 1258
Sau3AI GATC 1 cut(s) 928
Sau96I GGNCC 9 cut(s) 476, 607, 809, 817, 834, 898, 1073, 1074, 1235
SchI GAGTC 1 cut(s) 728
ScrFI CCNGG 1 cut(s) 807
SduI GDGCHC 6 cut(s) 531, 773, 848, 881, 990, 1077
SfiI GGCCNNNNNGGCC 1 cut(s) 816
SfuI TTCGAA 1 cut(s) 1034
SgrAI CRCCGGYG 1 cut(s) 417
SinI GGWCC 2 cut(s) 607, 898
SnaBI TACGTA 1 cut(s) 1002
Sse9I AATT 6 cut(s) 51, 739, 1030, 1196, 1250, 1255
SsiI CCGC 8 cut(s) 14, 257, 260, 263, 323, 731, 871, 911
StyD4I CCNGG 1 cut(s) 805
TaaI ACNGT 3 cut(s) 902, 948, 1111
TaiI ACGT 4 cut(s) 703, 1004, 1008, 1046
TaqI TCGA 2 cut(s) 891, 1034
TasI AATT 6 cut(s) 51, 739, 1030, 1196, 1250, 1255
TatI WGTACW 1 cut(s) 1152
TauI GCSGC 2 cut(s) 262, 265
TfiI GAWTC 1 cut(s) 1036
Tru1I TTAA 3 cut(s) 335, 738, 1258
Tru9I TTAA 3 cut(s) 335, 738, 1258
TscAI CASTG 5 cut(s) 411, 819, 890, 951, 965
TseFI GTSAC 2 cut(s) 622, 1105
Tsp45I GTSAC 2 cut(s) 622, 1105
TspDTI ATGAA 4 cut(s) 17, 198, 448, 537
TspGWI ACGGA 2 cut(s) 483, 1071
TspRI CASTG 5 cut(s) 411, 819, 890, 951, 965
VpaK11BI GGWCC 2 cut(s) 607, 898
XapI RAATTY 3 cut(s) 1030, 1196, 1250
XmiI GTMKAC 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.